@article{LassonczykVoigtGabrieletal.1990, author = {Lassonczyk, Beate and Voigt, B. and Gabriel, B. and Ghod, Mumin M.}, title = {Quaternary events at the Horn of Africa / Voigt, B., B. Gabriel, B. Lassonczyk and Mumin M. Ghod}, series = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, journal = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, isbn = {0172-8784}, pages = {679 -- 694}, year = {1990}, language = {en} } @article{MonakhovaSobolevaFedotovaetal.2022, author = {Monakhova, Yulia and Soboleva, Polina M. and Fedotova, Elena S. and Musina, Kristina T. and Burmistrova, Natalia A.}, title = {Quantum chemical calculations of IR spectra of heparin disaccharide subunits}, series = {Computational and Theoretical Chemistry}, volume = {1217}, journal = {Computational and Theoretical Chemistry}, number = {Article number: 113891}, publisher = {Elsevier}, address = {New York, NY}, isbn = {2210-271X}, doi = {10.1016/j.comptc.2022.113891}, year = {2022}, abstract = {Heparin is a natural polysaccharide, which plays essential role in many biological processes. Alterations in building blocks can modify biological roles of commercial heparin products, due to significant changes in the conformation of the polymer chain. The variability structure of heparin leads to difficulty in quality control using different analytical methods, including infrared (IR) spectroscopy. In this paper molecular modelling of heparin disaccharide subunits was performed using quantum chemistry. The structural and spectral parameters of these disaccharides have been calculated using RHF/6-311G. In addition, over-sulphated chondroitin sulphate disaccharide was studied as one of the most widespread contaminants of heparin. Calculated IR spectra were analyzed with respect to specific structure parameters. IR spectroscopic fingerprint was found to be sensitive to substitution pattern of disaccharide subunits. Vibrational assignments of calculated spectra were correlated with experimental IR spectral bands of native heparin. Chemometrics was used to perform multivariate analysis of simulated spectral data.}, language = {en} } @article{HasegawaKapelyukhTaharaetal.2011, author = {Hasegawa, Maki and Kapelyukh, Yury and Tahara, Harunobu and Seibler, Jost and Rode, Anja and Krueger, Sylvia and Lee, Dongtao N. and Wolf, C. Roland and Scheer, Nico}, title = {Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line}, series = {Molecular Pharmacology}, volume = {80}, journal = {Molecular Pharmacology}, number = {33}, publisher = {ASPET}, address = {Bethesda, Md.}, issn = {1521-0111}, doi = {10.1124/mol.111.071845}, pages = {518 -- 528}, year = {2011}, language = {en} } @article{TippkoetterRoth2020, author = {Tippk{\"o}tter, Nils and Roth, Jasmine}, title = {Purified Butanol from Lignocellulose - Solvent-Impregnated Resins for an Integrated Selective Removal}, series = {Chemie Ingenieur Technik}, volume = {92}, journal = {Chemie Ingenieur Technik}, number = {11}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1522-2640}, doi = {10.1002/cite.202000200}, pages = {1741 -- 1751}, year = {2020}, abstract = {In traditional microbial biobutanol production, the solvent must be recovered during fermentation process for a sufficient space-time yield. Thermal separation is not feasible due to the boiling point of n-butanol. As an integrated and selective solid-liquid separation alternative, solvent impregnated resins (SIRs) were applied. Two polymeric resins were evaluated and an extractant screening was conducted. Vacuum application with vapor collection in fixed-bed column as bioreactor bypass was successfully implemented as butanol desorption step. In course of further increasing process economics, fermentation with renewable lignocellulosic substrates was conducted using Clostridium acetobutylicum. Utilization of SIR was shown to be a potential strategy for solvent removal from fermentation broth, while application of a bypass column allows for product removal and recovery at once.}, language = {en} } @article{BiselliThoemmesBornetal.1995, author = {Biselli, Manfred and Th{\"o}mmes, J. and Born, C. and Wandrey, C.}, title = {Purification of monoclonal antibodies by fluidized bed adsorption / Th{\"o}mmes, J. ; Born, C. Biselli, M. ; Wandrey, C. ; Kula, M.-R.}, series = {Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery}, journal = {Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery}, publisher = {Kluwer Acad. Press}, address = {Dordrecht}, isbn = {0-7923-3736-0}, pages = {515 -- 519}, year = {1995}, language = {en} } @article{SchmitzHirschBongaertsetal.2002, author = {Schmitz, M. and Hirsch, E. and Bongaerts, Johannes and Takors, Ralf}, title = {Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli}, series = {Biotechnology progress}, volume = {Vol. 18}, journal = {Biotechnology progress}, number = {Iss. 5}, issn = {1520-6033 (E-Journal); 8756-7938 (Print)}, pages = {935 -- 941}, year = {2002}, language = {en} } @article{DellmannGloriusLitvinovetal.2023, author = {Dellmann, Sophia Florence and Glorius, J. and Litvinov, Yu A. and Reifarth, R. and Al-Khasawneh, Kafa and Aliotta, M. and Bott, L. and Br{\"u}ckner, Benjamin and Bruno, C. G. and Chen, Ruijiu and Davinson, T. and Dickel, T. and Dillmann, Iris and Dmytriev, D. and Erbacher, P. and Freire-Fern{\´a}ndez, D. and Forstner, Oliver and Geissel, H. and G{\"o}bel, K. and Griffin, Christopher J. and Grisenti, R. and Gumberidze, Alexandre and Haettner, Emma and Hagmann, Siegbert and Heil, M. and Heß, R. and Hillenbrand, P.-M. and Joseph, R. and Jurado, B. and Kozhuharov, Christophor and Kulikov, I. and L{\"o}her, Bastian and Langer, Christoph and Leckenby, Guy and Lederer-Woods, C. and Lestinsky, M. and Litvinov, S. A. and Lorenz, B. A. and Lorenz, E. and Marsh, J. and Menz, Esther Babette and Morgenroth, T. and Petridis, N. and Pibernat, Jerome and Popp, U. and Psaltis, Athanasios and Sanjari, Shahab and Scheidenberger, C. and Sguazzin, M. and Sidhu, Ragandeep Singh and Spillmann, Uwe and Steck, M. and St{\"o}hlker, T. and Surzhykov, A. and Swartz, J. A. and T{\"o}rnqvist, H. and Varga, L. and Vescovi, Diego and Weick, H. and Weigand, M. and Woods, P. and Xing, Y. and Yamaguchi, Taiyo}, title = {Proton capture on stored radioactive ¹¹⁸Te ions}, series = {EPJ Web of Conferences}, volume = {279}, journal = {EPJ Web of Conferences}, number = {Article Number: 11018}, publisher = {EDP Sciences}, issn = {2100-014X}, doi = {10.1051/epjconf/202327911018}, pages = {1 -- 5}, year = {2023}, abstract = {Experimental determination of the cross sections of proton capture on radioactive nuclei is extremely difficult. Therefore, it is of substantial interest for the understanding of the production of the p-nuclei. For the first time, a direct measurement of proton-capture cross sections on stored, radioactive ions became possible in an energy range of interest for nuclear astrophysics. The experiment was performed at the Experimental Storage Ring (ESR) at GSI by making use of a sensitive method to measure (p,γ) and (p,n) reactions in inverse kinematics. These reaction channels are of high relevance for the nucleosyn-thesis processes in supernovae, which are among the most violent explosions in the universe and are not yet well understood. The cross section of the ¹¹⁸Te(p,γ) reaction has been measured at energies of 6 MeV/u and 7 MeV/u. The heavy ions interacted with a hydrogen gas jet target. The radiative recombination process of the fully stripped ¹¹⁸Te ions and electrons from the hydrogen target was used as a luminosity monitor. An overview of the experimental method and preliminary results from the ongoing analysis will be presented.}, language = {en} } @article{SelmerWillanzheimerHetzel2002, author = {Selmer, Thorsten and Willanzheimer, Angela and Hetzel, Marc}, title = {Propionate CoA-transferase from Clostridium propionicum. Cloning of the gene and identification of glutamate 324 at the active site}, series = {European Journal of Biochemistry. 269 (2002), H. 1}, journal = {European Journal of Biochemistry. 269 (2002), H. 1}, isbn = {0014-2956}, pages = {372 -- 380}, year = {2002}, language = {en} } @article{SelmerAchebachUnden2005, author = {Selmer, Thorsten and Achebach, Stephanie and Unden, Gottfried}, title = {Properties and significance of apoFNR as a second form of air-inactivated [4Fe-4S]·FNR of Escherichia coli / Achebach, S. ; Selmer, T. ; Unden, G.}, series = {The FEBS Journal. 272 (2005), H. 16}, journal = {The FEBS Journal. 272 (2005), H. 16}, isbn = {1742-464X}, pages = {4260 -- 4269}, year = {2005}, language = {en} } @article{PaulssenHoehrHouetal.2015, author = {Paulßen, Elisabeth and Hoehr, Cornelia and Hou, Xinchi and Hanemaayer, Victoire and Zeisler, Stefan and Adam, Michael J. and Ruth, Thomas J. and Celler, Anna and Buckley, Ken and Benard, Francois and Schaffer, Paul}, title = {Production of Y-86 and other radiometals for research purposes using a solution target system}, series = {Nuclear medicine and biology}, volume = {42}, journal = {Nuclear medicine and biology}, number = {11}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1872-9614}, doi = {10.1016/j.nucmedbio.2015.06.005}, pages = {842 -- 849}, year = {2015}, language = {en} } @article{BiselliBornWandrey1995, author = {Biselli, Manfred and Born, C. and Wandrey, C.}, title = {Production of monoclonal antibodies in a pilot scale fluidized bed bioreactor / Born, C.; Biselli, M.; Wandrey, C.}, series = {Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery}, journal = {Animal cell technology : developments towards the 21st century / edited by E. C. Beuvery}, publisher = {Kluwer Acad. Press}, address = {Dordrecht}, isbn = {0-7923-3736-0}, pages = {515 -- 519}, year = {1995}, language = {en} } @article{GerigkBujnickiGanpoNkwenkwaetal.2002, author = {Gerigk, M. and Bujnicki, R. and Ganpo-Nkwenkwa, E. and Bongaerts, Johannes and Sprenger, G. and Takors, Ralf}, title = {Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains}, series = {Biotechnology and bioengineering}, volume = {Vol. 80}, journal = {Biotechnology and bioengineering}, number = {Iss. 7}, issn = {1097-0290 (E-Journal); 0006-3592 (Print)}, pages = {746 -- 754}, year = {2002}, language = {en} } @article{TuegBaumann1994, author = {T{\"u}g, Helmut and Baumann, Marcus}, title = {Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements}, series = {Geophysical research letters}, volume = {Vol. 21}, journal = {Geophysical research letters}, number = {Iss. 8}, issn = {1944-8007 (E-Journal); 0094-8276 (Print)}, pages = {689 -- 692}, year = {1994}, language = {en} } @article{PrielmeierLangLuedemann1984, author = {Prielmeier, Franz and Lang, E. W. and L{\"u}demann, H.-D.}, title = {Pressure dependence of the self-diffusion in liquid trifluoromethane / F. X. Prielmeier; E. W. Lang; H.-D. L{\"u}demann}, series = {Molecular Physics. 52 (1984), H. 5}, journal = {Molecular Physics. 52 (1984), H. 5}, isbn = {0026-8976}, pages = {1105 -- 1113}, year = {1984}, language = {en} } @article{PrielmeierWoznyjLuedemann1984, author = {Prielmeier, Franz and Woznyj, M. and L{\"u}demann, H.-D.}, title = {Pressure Dependence of the Melting and Self Diffusion in 2,2-Dimethylpropane, 2,2-Dimethylpropionitrile, and 2-Methylpropanol-2 / M. Woznyj, F. X. Prielmeier, H.-D. L{\"u}demann}, series = {Zeitschrift f{\"u}r Naturforschung A, Journal of Physical Sciences. 39 (1984)}, journal = {Zeitschrift f{\"u}r Naturforschung A, Journal of Physical Sciences. 39 (1984)}, isbn = {0932-0784}, pages = {800}, year = {1984}, language = {en} } @article{NaithaniKlostermeyerLangeetal.1971, author = {Naithani, V. K and Klostermeyer, Henning and Lange, H. R. and [u.a.], and Berndt, Heinz and [u.a.],}, title = {Preparation of peptide derivatives for porcine proinsulin-synthesis}, series = {Biological Chemistry}, volume = {352}, journal = {Biological Chemistry}, number = {1}, publisher = {De Gruyter}, issn = {1437-4315}, doi = {10.1515/bchm2.1971.352.1.1}, pages = {2 -- 3}, year = {1971}, language = {en} } @article{KotterHammon1986, author = {Kotter, Michael and Hammon, U.}, title = {Preparation of pellets with well-defined pore structure / U. Hammon ; A. Kotter}, series = {International chemical engineering. 26 (1986), H. 4}, journal = {International chemical engineering. 26 (1986), H. 4}, isbn = {0020-6318}, pages = {563 -- 573}, year = {1986}, language = {en} } @article{BiselliSchmidWandrey1988, author = {Biselli, Manfred and Schmid, Georg and Wandrey, Christian}, title = {Preparation of cellodextrins and isolation of oligomeric side components and their characterization / Schmid, Georg ; Biselli, Manfred ; Wandrey, Christian}, series = {Analytical Biochemistry. 175 (1988)}, journal = {Analytical Biochemistry. 175 (1988)}, isbn = {0003-2697}, pages = {573 -- 583}, year = {1988}, language = {en} } @article{HenkenOosterhuisOehlschlaegeretal.2012, author = {Henken, F. E. and Oosterhuis, K. and {\"O}hlschl{\"a}ger, Peter and Bosch, L. and Hooijberg, E. and Haanen, J. B. A. G. and Steenbergen, R. D. M.}, title = {Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7}, series = {Vaccine}, volume = {30}, journal = {Vaccine}, number = {28}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0264-410X}, doi = {10.1016/j.vaccine.2012.04.013}, pages = {4259 -- 4266}, year = {2012}, abstract = {Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of 'gene-shuffled' (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.}, language = {en} } @article{OosterhuisOehlschlaegerBergetal.2011, author = {Oosterhuis, Koen and {\"O}hlschl{\"a}ger, Peter and Berg, Joost H. van den and Toebes, Mireille and Gomez, Raquel and Schumacher, Ton N. and Haanen, John B.}, title = {Preclinical development of highly effective and safe DNA vaccines directed against HPV 16 E6 and E7}, series = {International Journal of Cancer}, volume = {129}, journal = {International Journal of Cancer}, number = {2}, publisher = {Wiley}, address = {Weinheim}, isbn = {1097-0215}, pages = {397 -- 406}, year = {2011}, language = {en} } @article{Mang2004, author = {Mang, Thomas}, title = {Polymer Magnetic Particles for Isolating Biomolecules, Especially Nucleic Acids}, series = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, journal = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, isbn = {0013-5585}, pages = {1008 -- 1009}, year = {2004}, language = {en} } @article{MangKricheldorfJonte1984, author = {Mang, Thomas and Kricheldorf, Hans R. and Jont{\´e}, J. Michael}, title = {Polylactones. 1. Copolymerizations of glycolide and \&beta-caprolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jont{\´e}, J. Michael}, series = {Macromolecules. 17 (1984), H. 10}, journal = {Macromolecules. 17 (1984), H. 10}, isbn = {0024-9297}, pages = {2173 -- 2181}, year = {1984}, language = {en} } @article{MangKricheldorfJonte1985, author = {Mang, Thomas and Kricheldorf, Hans R. and Jont{\´e}, J. Michael}, title = {Polylactones, 2. Copolymerization of glycolide with \&\#946;-propiolactone, \&\#967;-butyrolactone or \&\#948;-valerolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jont{\´e}, J. Michael}, series = {Die makromolekulare Chemie. 186 (1985), H. 5}, journal = {Die makromolekulare Chemie. 186 (1985), H. 5}, isbn = {1022-1352}, pages = {955 -- 976}, year = {1985}, language = {en} } @article{MichalakNacerddinePietersenetal.2013, author = {Michalak, Ewa Malgorzata and Nacerddine, Karim and Pietersen, Alexandra and Beuger, Vincent and Pawlitzky, Inka and Cornelissen-Steijger, Paulien and Wientjens, Ellen and Tanger, Ellen and Seibler, Jost and Lohuizen, Maarten van and Jonkers, Jos}, title = {Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool}, series = {Stem Cells}, volume = {Vol 31}, journal = {Stem Cells}, number = {9}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1549-4918}, doi = {10.1002/stem.1437}, pages = {1910 -- 1920}, year = {2013}, language = {en} } @article{BaumannHircheKattneretal.1991, author = {Baumann, Marcus and Hirche, H.-J. and Kattner, G. and Gradinger, R.}, title = {Plankton distribution and the impact of copepod grazing on primary production in Fram Strait, Greenland Sea / Hirche, H.-J. ; Baumann, M.E.M. ; Kattner, G.; Gradinger, R.}, series = {Journal of Marine Systems. 2 (1991), H. 3-4}, journal = {Journal of Marine Systems. 2 (1991), H. 3-4}, isbn = {0924-7963}, pages = {477 -- 494}, year = {1991}, language = {en} } @article{BiselliZengDinteretal.1997, author = {Biselli, Manfred and Zeng, Steffen and Dinter, Andre and Eisenkr{\"a}tzer, Detlef}, title = {Pilot Scale Expression and Purification of Soluble Protein A Tagged \&\#946;1,6N-Acetylglucosaminyltransferase in CHO Cells / Zeng, Steffen ; Dinter, Andre ; Eisenkr{\"a}tzer, Detlef ; Biselli, Manfred ; Wandrey, Christian ; Berger, Eric G.}, series = {Biochemical and Biophysical Research Communications. 237 (1997), H. 3}, journal = {Biochemical and Biophysical Research Communications. 237 (1997), H. 3}, isbn = {0006-291X}, pages = {653 -- 658}, year = {1997}, language = {en} } @article{BaumannGoeyensSemenehetal.1998, author = {Baumann, Marcus and Goeyens, L. and Semeneh, M. and Elskens, M.}, title = {Phytoplankton nutrient utilisation and nutrient signature in the Southern Ocean / Goeyens, L. ; Semeneh, M. ; Baumann, M.E.M. ; Elskens, M. ; Shopova, D. ; Dehairs, F.}, series = {Journal of Marine Systems. 17 (1998), H. 1-4}, journal = {Journal of Marine Systems. 17 (1998), H. 1-4}, isbn = {0924-7963}, pages = {143 -- 157}, year = {1998}, language = {en} } @article{SmithBaumannWilsonetal.1987, author = {Smith, Walker O. and Baumann, Marcus and Wilson, David L. and Aletsee, Ludwig}, title = {Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984}, series = {Journal of geophysical research}, volume = {Vol. 92}, journal = {Journal of geophysical research}, number = {Iss. C7}, issn = {2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print)}, pages = {6777 -- 6786}, year = {1987}, language = {en} } @article{AggarwalSinghSinghetal.2011, author = {Aggarwal, Prerna and Singh, Virendra and Singh, Arjun and Scherer, Ulrich W. and Singh, Tejvir and Singla, Madan L. and Srivastava, Alok}, title = {Physico-chemical transformations in swift heavy ion modified poly(ethyleneterephthalate)}, series = {Radiation Physics and Chemistry}, journal = {Radiation Physics and Chemistry}, publisher = {Pergamon Press}, address = {Oxford}, isbn = {0969-806X}, pages = {1 -- 28}, year = {2011}, language = {en} } @article{FalkenbergBottBongaertsetal.2022, author = {Falkenberg, Fabian and Bott, Michael and Bongaerts, Johannes and Siegert, Petra}, title = {Phylogenetic survey of the subtilase family and a data-mining-based search for new subtilisins from Bacillaceae}, series = {Frontiers in Microbiology}, volume = {2022}, journal = {Frontiers in Microbiology}, number = {13}, publisher = {Frontiers}, address = {Lausanne}, issn = {1664-302X}, doi = {10.3389/fmicb.2022.1017978}, pages = {Artikel 13:1017978}, year = {2022}, abstract = {The subtilase family (S8), a member of the clan SB of serine proteases are ubiquitous in all kingdoms of life and fulfil different physiological functions. Subtilases are divided in several groups and especially subtilisins are of interest as they are used in various industrial sectors. Therefore, we searched for new subtilisin sequences of the family Bacillaceae using a data mining approach. The obtained 1,400 sequences were phylogenetically classified in the context of the subtilase family. This required an updated comprehensive overview of the different groups within this family. To fill this gap, we conducted a phylogenetic survey of the S8 family with characterised holotypes derived from the MEROPS database. The analysis revealed the presence of eight previously uncharacterised groups and 13 subgroups within the S8 family. The sequences that emerged from the data mining with the set filter parameters were mainly assigned to the subtilisin subgroups of true subtilisins, high-alkaline subtilisins, and phylogenetically intermediate subtilisins and represent an excellent source for new subtilisin candidates.}, language = {en} } @article{LuisierLempiaeinenScherbichleretal.2014, author = {Luisier, Rapha{\"e}lle and Lempi{\"a}inen, Harri and Scherbichler, Nina and Braeuning, Albert and Geissler, Miriam and Dubost, Valerie and M{\"u}ller, Arne and Scheer, Nico and Chibout, Salah-Dine and Hara, Hisanori and Picard, Frank and Theil, Diethilde and Couttet, Philippe and Vitobello, Antonio and Grenet, Olivier and Grasl-Kraupp, Bettina and Ellinger-Ziegelbauer, Heidrung and Thomson, John P. and Meehan, Richard R. and Elcombe, Clifford R. and Henderson, Colin J. and Wolf, C. Roland and Schwarz, Michael and Moulin, Pierre and Terranova, Remi and Moggs, Jonathan G.}, title = {Phenobarbital Induces Cell Cycle Transcriptional Responses in Mouse Liver Humanized for Constitutive Androstane and Pregnane X Receptors}, series = {Toxicological Sciences}, volume = {139}, journal = {Toxicological Sciences}, number = {2}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1094-2025}, doi = {https://doi.org/10.1093/toxsci/kfu038}, pages = {501 -- 511}, year = {2014}, abstract = {The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CARᴷᴼ-PXRᴷᴼ), double humanized CAR and PXR (CARʰ-PXRʰ), and wild-type C57BL/6 mice. Wild-type and CARʰ-PXRʰ mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CARᴷᴼ-PXRᴷᴼ mouse livers and largely reversible in wild-type and CARʰ-PXRʰ mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CARʰ-PXRʰ mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB.}, language = {en} } @article{OehlschlaegerCorvinusOrthetal.2005, author = {{\"O}hlschl{\"a}ger, Peter and Corvinus, Florian M. and Orth, Carina and Moriggl, Richard}, title = {Persistent STAT3 activation in colon cancer is associated with enhanced cell proliferation and tumor growth / Corvinus, Florian, Moriggl, Richard ; Tsareva, Svetlana A. ; Wagner, Stefan ; Pfitzner, Edith B. ; Baus, Daniela ; Kaufmann, Roland : Huber, Luka}, series = {Neoplasia. 7 (2005), H. 6}, journal = {Neoplasia. 7 (2005), H. 6}, isbn = {1476-5586}, pages = {545 -- 555}, year = {2005}, language = {en} } @article{FeuerriegelStahlberg1996, author = {Feuerriegel, Uwe and Stahlberg, R.}, title = {Performance of the Thermoselect Demonstration Plant at Fondotoce, Italy: Product Quality and Distribution of Chemical Elements in the Material Flow. Stahlberg, R. ; Feuerriegel, U.}, series = {Solid waste management: thermal treatment \& waste-to-energy technologies : proceedings of an international specialty conference sponsored by the Air \& Waste Management Association, [Washington, DC, April 18 - 21, 1995] / comp. by James D. Kilgroe}, journal = {Solid waste management: thermal treatment \& waste-to-energy technologies : proceedings of an international specialty conference sponsored by the Air \& Waste Management Association, [Washington, DC, April 18 - 21, 1995] / comp. by James D. Kilgroe}, publisher = {Air \& Waste Management Assoc.}, address = {Pittsburgh, Pa.}, pages = {XVI, 965 S : graph. Darst.}, year = {1996}, language = {en} } @article{GoetzeBaerWinkelmannetal.2005, author = {Goetze, Sandra and Baer, Alexandra and Winkelmann, Silke and Nehlsen, Kristina and Seibler, Jost and Maass, Karin and Bode, J{\"u}rgen}, title = {Performance of genomic bordering elements at predefined genomic loci}, series = {Molecular and Cellular Biology}, volume = {25}, journal = {Molecular and Cellular Biology}, number = {6}, issn = {1098-5549}, doi = {10.1128/MCB.25.6.2260-2272.2005}, pages = {2260 -- 2272}, year = {2005}, language = {en} } @article{KotterLiRiekert1990, author = {Kotter, Michael and Li, D. X. and Riekert, Lothar}, title = {Partial oxidation of o-xylene to phthalic anhydride in a structured fixed bed containing a sequence of catalysts}, series = {New developments in selective oxidation : proceedings of an international symposium ; Rimini, Italy, Sept. 18 - 22, 1989 / eds.: G. Centi ... - (Studies in surface science and catalysis ; 55)}, journal = {New developments in selective oxidation : proceedings of an international symposium ; Rimini, Italy, Sept. 18 - 22, 1989 / eds.: G. Centi ... - (Studies in surface science and catalysis ; 55)}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0-444-88694-X}, pages = {267 -- 274}, year = {1990}, language = {en} } @article{TippkoetterRoikaewUlberetal.2010, author = {Tippk{\"o}tter, Nils and Roikaew, Wipa and Ulber, Roland and Hoffmann, Alexander and Denzler, Hans-J{\"o}rg and Buchholz, Heinrich}, title = {Paracoccus denitrificans for the effluent recycling during continuous denitrification of liquid food}, series = {Biotechnology Progress}, volume = {26}, journal = {Biotechnology Progress}, number = {3}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {8756-7938}, doi = {10.1002/btpr.384}, pages = {756 -- 762}, year = {2010}, abstract = {Nitrate is an undesirable component of several foods. A typical case of contamination with high nitrate contents is whey concentrate, containing nitrate in concentrations up to 25 l. The microbiological removal of nitrate by Paracoccus denitrificans under formation of harmless nitrogen in combination with a cell retention reactor is described here. Focus lies on the resource-conserving design of a microbal denitrification process. Two methods are compared. The application of polyvinyl alcohol-immobilized cells, which can be applied several times in whey feed, is compared with the implementation of a two step denitrification system. First, the whey concentrate's nitrate is removed by ion exchange and subsequently the eluent regenerated by microorganisms under their retention by crossflow filtration. Nitrite and nitrate concentrations were determined by reflectometric color measurement with a commercially available Reflectoquant® device. Correction factors for these media had to be determined. During the pilot development, bioreactors from 4 to 250 mg·L-1 and crossflow units with membrane areas from 0.02 to 0.80 m2 were examined. Based on the results of the pilot plants, a scaling for the exemplary process of denitrifying 1,000 tons per day is discussed.}, language = {en} } @article{SelmerAndrei2001, author = {Selmer, Thorsten and Andrei, Paula I.}, title = {p-Hydroxyphenylacetate decarboxylase from Clostridium difficile. A novel glycyl radical enzyme catalysing the formation of p-cresol}, series = {European Journal of Biochemistry. 268 (2001), H. 5}, journal = {European Journal of Biochemistry. 268 (2001), H. 5}, isbn = {0014-2956}, pages = {1363 -- 1372}, year = {2001}, language = {en} } @article{BiselliBornWandrey1995, author = {Biselli, Manfred and Born, C. and Wandrey, C.}, title = {Oxygen transfer from the gasphase to the immobilized cells in membrane aerated fluidized beds / Born, C. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : basic \& applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita}, journal = {Animal cell technology : basic \& applied aspects : proceedings of the Eighth Annual Meeting of the Japanese Association for Animal Cell Technology, Iizuka, Fukuoka, Japan, November 6-10, 1995 / edited by K. Funatsu, Y. Shirai, and T. Matsushita}, publisher = {Kluwer Acad. Press}, address = {Boston}, isbn = {0-7923-4486-3}, pages = {83 -- 87}, year = {1995}, language = {en} } @article{UndenBeckerBongaertsetal.1994, author = {Unden, G. and Becker, S. and Bongaerts, Johannes and Schirawski, J. and Six, S.}, title = {Oxygen regulated gene expression in facultatively anaerobic bacteria}, series = {Antonie van Leeuwenhoek}, volume = {Vol. 66}, journal = {Antonie van Leeuwenhoek}, number = {Iss. 1-3}, issn = {0003-6072 (Print) ; 1572-9699 (online)}, pages = {3 -- 22}, year = {1994}, language = {en} } @article{SelmerBuckel1999, author = {Selmer, Thorsten and Buckel, Wolfgang}, title = {Oxygen Exchange between Acetate and the Catalytic Glutamate Residue in Glutaconate CoA-transferase from Acidaminococcus fermentans. IMPLICATIONS FOR THE MECHANISM OF CoA-ESTER HYDROLYSIS}, series = {Journal of Biological Chemistry. 274 (1999), H. 30}, journal = {Journal of Biological Chemistry. 274 (1999), H. 30}, isbn = {1083-351X}, pages = {20772 -- 20778}, year = {1999}, language = {en} } @article{PennerUsherovichNiedermeieretal.2022, author = {Penner, Crystal and Usherovich, Samuel and Niedermeier, Jana and B{\´e}langer-Champagne, Camille and Trinczek, Michael and Paulßen, Elisabeth and Hoehr, Cornelia}, title = {Organic Scintillator-Fibre Sensors for Proton Therapy Dosimetry: SCSF-3HF and EJ-260}, series = {electronics}, volume = {12}, journal = {electronics}, number = {1}, publisher = {MDPI}, address = {Basel}, issn = {2079-9292}, doi = {10.3390/electronics12010011}, pages = {12 Seiten}, year = {2022}, abstract = {In proton therapy, the dose from secondary neutrons to the patient can contribute to side effects and the creation of secondary cancer. A simple and fast detection system to distinguish between dose from protons and neutrons both in pretreatment verification as well as potentially in vivo monitoring is needed to minimize dose from secondary neutrons. Two 3 mm long, 1 mm diameter organic scintillators were tested for candidacy to be used in a proton-neutron discrimination detector. The SCSF-3HF (1500) scintillating fibre (Kuraray Co. Chiyoda-ku, Tokyo, Japan) and EJ-260 plastic scintillator (Eljen Technology, Sweetwater, TX, USA) were irradiated at the TRIUMF Neutron Facility and the Proton Therapy Research Centre. In the proton beam, we compared the raw Bragg peak and spread-out Bragg peak response to the industry standard Markus chamber detector. Both scintillator sensors exhibited quenching at high LET in the Bragg peak, presenting a peak-to-entrance ratio of 2.59 for the EJ-260 and 2.63 for the SCSF-3HF fibre, compared to 3.70 for the Markus chamber. The SCSF-3HF sensor demonstrated 1.3 times the sensitivity to protons and 3 times the sensitivity to neutrons as compared to the EJ-260 sensor. Combined with our equations relating neutron and proton contributions to dose during proton irradiations, and the application of Birks' quenching correction, these fibres provide valid candidates for inexpensive and replicable proton-neutron discrimination detectors}, language = {en} } @article{HoffstadtNikolauszKrafftetal.2024, author = {Hoffstadt, Kevin and Nikolausz, Marcell and Krafft, Simone and Bonatelli, Maria and Kumar, Vivekanantha and Harms, Hauke and Kuperjans, Isabel}, title = {Optimization of the ex situ biomethanation of hydrogen and carbon dioxide in a novel meandering plug flow reactor: start-up phase and flexible operation}, series = {Bioengineering}, volume = {11}, journal = {Bioengineering}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2306-5354}, doi = {10.3390/bioengineering11020165}, pages = {18 Seiten}, year = {2024}, language = {en} } @article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @article{PasteurTippkoetterKampeisetal.2014, author = {Pasteur, Aline and Tippk{\"o}tter, Nils and Kampeis, Percy and Ulber, Roland}, title = {Optimization of high gradient magnetic separation filter units for the purification of fermentation products}, series = {IEEE TRANSACTIONS ON MAGNETICS}, volume = {50}, journal = {IEEE TRANSACTIONS ON MAGNETICS}, number = {10}, publisher = {IEEE}, address = {New York, NY}, issn = {0018-9464}, doi = {10.1109/TMAG.2014.2325535}, pages = {Artikel 5000607}, year = {2014}, abstract = {High gradient magnetic separation (HGMS) has been established since the early 1970s. A more recent application of these systems is the use in bioprocesses. To integrate the HGMS in a fermentation process, it is necessary to optimize the separation matrix with regard to the magnetic separation characteristics and permeability of the non-magnetizable components of the fermentation broth. As part of the work presented here, a combined fluidic and magnetic force finite element model simulation was created using the software COMSOL Multiphysics and compared with separation experiments. Finally, as optimal lattice orientation of the separation matrix, a transversal rhombohedral arrangement was defined. The high suitability of the new filter matrix has been verified by separation experiments.}, language = {en} } @article{BiselliRolefDunkeretal.1994, author = {Biselli, Manfred and Rolef, G. and Dunker, R. and Wandrey, Christian}, title = {Optimization of antibody production in a fluidized bed bioreactor / Rolef, G. ; Biselli, M. ; Dunker, R. ; Wandrey, C.}, series = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750618450}, pages = {481 -- 484}, year = {1994}, language = {en} } @article{PilasMarianoKeusgenetal.2015, author = {Pilas, Johanna and Mariano, K. and Keusgen, M. and Selmer, Thorsten and Sch{\"o}ning, Michael Josef}, title = {Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes}, series = {Procedia Engineering}, volume = {120}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2015.08.702}, pages = {532 -- 535}, year = {2015}, language = {en} } @article{PilasYaziciSelmeretal.2017, author = {Pilas, Johanna and Yazici, Yasemen and Selmer, Thorsten and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Optimization of an amperometric biosensor array for simultaneous measurement of ethanol, formate, d- and l-lactate}, series = {Electrochimica Acta}, volume = {251}, journal = {Electrochimica Acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2017.07.119}, pages = {256 -- 262}, year = {2017}, abstract = {The immobilization of NAD+-dependent dehydrogenases, in combination with a diaphorase, enables the facile development of multiparametric sensing devices. In this work, an amperometric biosensor array for simultaneous determination of ethanol, formate, d- and l-lactate is presented. Enzyme immobilization on platinum thin-film electrodes was realized by chemical cross-linking with glutaraldehyde. The optimization of the sensor performance was investigated with regard to enzyme loading, glutaraldehyde concentration, pH, cofactor concentration and temperature. Under optimal working conditions (potassium phosphate buffer with pH 7.5, 2.5 mmol L-1 NAD+, 2.0 mmol L-1 ferricyanide, 25 °C and 0.4\% glutaraldehyde) the linear working range and sensitivity of the four sensor elements was improved. Simultaneous and cross-talk free measurements of four different metabolic parameters were performed successfully. The reliable analytical performance of the biosensor array was demonstrated by application in a clarified sample of inoculum sludge. Thereby, a promising approach for on-site monitoring of fermentation processes is provided.}, language = {en} } @article{JerominAlbertz1992, author = {Jeromin, G{\"u}nter Erich and Albertz, Michael}, title = {Optically active \&\#945;-acetoxycarboxylic acids and \&\#945;-hydroxycarboxylic acids by enzyme-aided syntheses}, series = {Journal f{\"u}r Praktische Chemie / Chemiker-Zeitung. 334 (1992), H. 6}, journal = {Journal f{\"u}r Praktische Chemie / Chemiker-Zeitung. 334 (1992), H. 6}, isbn = {1436-9966}, pages = {526 -- 528}, year = {1992}, language = {en} } @article{AggarwalDhimanKumaretal.2012, author = {Aggarwal, P. and Dhiman, S. and Kumar, G. and Scherer, Ulrich W. and Singla, M. L. and Srivastava, A.}, title = {Optical study of poly(ethyleneterephthalate) modified by different ionizing radiation dose}, series = {Indian Journal of Pure and Applied Physics}, volume = {50}, journal = {Indian Journal of Pure and Applied Physics}, number = {2}, issn = {0019-5596}, pages = {129 -- 132}, year = {2012}, abstract = {Thin films of poly(ethyleneterephthalate) [PET]were exposed to radiation dose ranging from 10 to 30 kGy by using gamma rays in the range 12.8-177.8 MGy using swift light ions of hydrogen. There was no effect of the radiation dose on the optical behaviour of PET as a result of exposure to radiation dose up to 30 kGy brought about by gamma rays but a significant decrease in the optical band gap values was observed when PET was exposed to swift light ions of hydrogen. The data obtained are discussed in terms of optical studies carried out on PET using swift heavy ions.}, language = {en} } @article{SrivastavaSinghAggarwaletal.2010, author = {Srivastava, A. and Singh, V. and Aggarwal, P. and Schneeweiss, F. and Scherer, Ulrich W. and Friedrich, W.}, title = {Optical studies of insulating polymers for radiation dose monitoring}, series = {Indian Journal of Pure \& Applied Physics}, volume = {48}, journal = {Indian Journal of Pure \& Applied Physics}, number = {11}, isbn = {0019-5596}, pages = {782 -- 786}, year = {2010}, language = {en} } @article{NiedermeierPennerUsherovichetal.2023, author = {Niedermeier, Jana and Penner, Crystal and Usherovich, Samuel and B{\´e}langer-Champagne, Camille and Paulßen, Elisabeth and Cornelia, Hoehr}, title = {Optical Fibers as Dosimeter Detectors for Mixed Proton/Neutron Fields - A Biological Dosimeter}, series = {electronics}, volume = {12}, journal = {electronics}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2079-9292}, doi = {10.3390/electronics12020324}, pages = {11 Seiten}, year = {2023}, abstract = {In recent years, proton therapy has gained importance as a cancer treatment modality due to its conformality with the tumor and the sparing of healthy tissue. However, in the interaction of the protons with the beam line elements and patient tissues, potentially harmful secondary neutrons are always generated. To ensure that this neutron dose is as low as possible, treatment plans could be created to also account for and minimize the neutron dose. To monitor such a treatment plan, a compact, easy to use, and inexpensive dosimeter must be developed that not only measures the physical dose, but which can also distinguish between proton and neutron contributions. To that end, plastic optical fibers with scintillation materials (Gd₂O₂S:Tb, Gd₂O₂S:Eu, and YVO₄:Eu) were irradiated with protons and neutrons. It was confirmed that sensors with different scintillation materials have different sensitivities to protons and neutrons. A combination of these three scintillators can be used to build a detector array to create a biological dosimeter.}, language = {en} } @article{HoughNalwalkDingetal.2015, author = {Hough, Lindsay B. and Nalwalk, Julia W. and Ding, Xinxin and Scheer, Nico}, title = {Opioid Analgesia in P450 Gene Cluster Knockout Mice: A Search for Analgesia-Relevant Isoforms}, series = {Drug Metabolism and Disposition}, volume = {43}, journal = {Drug Metabolism and Disposition}, number = {9}, issn = {1521-009x}, doi = {10.1124/dmd.115.065490}, pages = {1326 -- 1330}, year = {2015}, language = {en} } @article{BiselliVanderPolSpohnetal.1994, author = {Biselli, Manfred and Van der Pol, Jens J. and Spohn, Uwe and Eberhardt, Rolf}, title = {On-line monitoring of an animal cell culture with multi-channel flow injection analysis / van der Pol, Jens J.; Spohn, Uwe ; Eberhardt, Rolf ; G{\"a}tgens, Jochen ; Biselli, Manfred ; Wandrey, Christian ; Tramper, Johannes}, series = {Journal of Biotechnology. 37 (1994), H. 3}, journal = {Journal of Biotechnology. 37 (1994), H. 3}, isbn = {0168-1656}, pages = {253 -- 264}, year = {1994}, language = {en} } @article{BiselliVanderPolJokschetal.1994, author = {Biselli, Manfred and Van der Pol, Jens J. and Joksch, B. and Spohn, U.}, title = {On-line monitoring and control of glucose, glutamine, lactate and ammonium during a high-cell-density cultivation of hybridoma cells / van der Pol, Jens J. ; Joksch, B. ; Spohn, U. ; Biselli, M. ; Wandrey, C.}, series = {Proceedings of the sixth international meeting of the Japanese Association for Animal Cell Technology [JAACT 93]: Nagoya, Japan, November 9 - 12, 1993 / ed. by T. Kobayashi}, journal = {Proceedings of the sixth international meeting of the Japanese Association for Animal Cell Technology [JAACT 93]: Nagoya, Japan, November 9 - 12, 1993 / ed. by T. Kobayashi}, publisher = {Springer Netherland}, address = {Berlin}, isbn = {0-7923-3156-7}, pages = {167 -- 170}, year = {1994}, language = {en} } @article{BiselliVanderPolMachniketal.1997, author = {Biselli, Manfred and Van der Pol, Jens J. and Machnik, Marc and Portela-Klein, Theresa}, title = {On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells / Jens J. van der Pol, Marc Machnik, Manfred Biselli, Theresa Portela-Klein, Cornelis D. de Gooijer, Johannes Tramper und Christian Wandrey}, series = {Cytotechnology. 24 (1997), H. 1}, journal = {Cytotechnology. 24 (1997), H. 1}, isbn = {0920-9069}, pages = {19 -- 30}, year = {1997}, language = {en} } @article{BiselliVanderPolJokschetal.1995, author = {Biselli, Manfred and Van der Pol, Jens J. and Joksch, Burkhard and Galgens, Jochem}, title = {On-line control of an immobilized hybridoma culture with multi-channel flow injektion analysis / Jens J. van der Pol, Burkhard Joksch, Jochem Galgens, Manfred Biselli, Cornelis D. de Gooijer, Johannes Tramper and Christian Wandrey}, series = {Journal of Biotechnology. 43 (1995), H. 3}, journal = {Journal of Biotechnology. 43 (1995), H. 3}, isbn = {0168-1656}, pages = {229 -- 242}, year = {1995}, language = {en} } @article{BiselliNollWandrey1997, author = {Biselli, Manfred and Noll, T. and Wandrey, C.}, title = {On-line biomass monitoring of immobilized hybridoma cells by dielectrical measurements / Noll, T. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo}, journal = {Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo}, publisher = {Kluwer}, address = {Dordrecht}, isbn = {0-7923-4321-2}, pages = {289 -- 294}, year = {1997}, language = {en} } @article{RatkeMilowLisinskietal.2014, author = {Ratke, Lorenz and Milow, Barbara and Lisinski, Susanne and Hoepfner, Sandra}, title = {On an effect of fine ceramic particles on the structure of aerogels}, series = {Microgravity science and technology}, volume = {26}, journal = {Microgravity science and technology}, publisher = {Springer Nature}, address = {Heidelberg}, issn = {0938-0108 ; 1875-0494}, doi = {10.1007/s12217-014-9380-2}, pages = {103 -- 110}, year = {2014}, language = {en} } @article{JordanKruegerWillmesetal.2011, author = {Jordan, Sabine D. and Kr{\"u}ger, Markus and Willmes, Diana M. and Redemann, Nora and Wunderlich, F. Thomas and Br{\"o}nneke, Hella S. and Merkwirth, Carsten and Kashkar, Hamid and Olkkonen, Vesa M. and B{\"o}ttger, Thomas and Braun, Thomas and Seibler, Jost and Br{\"u}ning, Jens C.}, title = {Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism}, series = {Nature Cell Biology}, volume = {13}, journal = {Nature Cell Biology}, number = {4}, publisher = {Nature}, address = {New York}, issn = {1465-7392}, doi = {10.1038/ncb2211}, pages = {434 -- 446}, year = {2011}, abstract = {The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143-145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143-ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.}, language = {en} } @article{KornfeldBaitzelKoenneretal.2013, author = {Kornfeld, Jan-Wilhelm and Baitzel, Catherina and K{\"o}nner, A. Christine and Nicholls, Hayley T. and Vogt, Merly C. and Herrmanns, Karolin and Scheja, Ludger and Haumaitre, C{\´e}cile and Wolf, Anna M. and Knippschild, Uwe and Seibler, Jost and Cereghini, Silvia and Heeren, Joerg and Stoffel, Markus and Br{\"u}ning, Jens C.}, title = {Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b}, series = {Nature}, volume = {494}, journal = {Nature}, number = {7435}, publisher = {Springer Nature}, address = {Cham}, isbn = {0028-0836}, doi = {10.1038/nature11793}, pages = {111 -- 115}, year = {2013}, language = {en} } @article{UndenBeckerBongaertsetal.1995, author = {Unden, G. and Becker, S. and Bongaerts, Johannes and Holighaus, G. and Schirawski, J. and Six, S.}, title = {O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria}, series = {Archives of microbiology}, volume = {Vol. 164}, journal = {Archives of microbiology}, number = {Iss. 2}, issn = {1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)}, pages = {81 -- 90}, year = {1995}, language = {en} } @article{WernerGroebelKrumbeetal.2012, author = {Werner, Frederik and Groebel, Simone and Krumbe, Christoph and Wagner, Torsten and Selmer, Thorsten and Yoshinobu, Tatsuo and Baumann, Marcus and Sch{\"o}ning, Michael Josef}, title = {Nutrient concentration-sensitive microorganism-based biosensor}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100801}, pages = {900 -- 904}, year = {2012}, language = {en} } @article{StanleyHorsburghRossetal.2006, author = {Stanley, Lesley A. and Horsburgh, Brian C. and Ross, Jillian and Scheer, Nico and Wolf, C. Roland}, title = {Nuclear Receptors which play a pivotal role in drug disposition and chemical toxicity}, series = {Drug Metabolism Reviews}, volume = {38}, journal = {Drug Metabolism Reviews}, number = {3}, issn = {1097-9883}, doi = {10.1080/03602530600786232}, pages = {515 -- 597}, year = {2006}, language = {en} } @article{MonakhovaDiehl2022, author = {Monakhova, Yulia and Diehl, Bernd W.K.}, title = {Nuclear magnetic resonance spectroscopy as an elegant tool for a complete quality control of crude heparin material}, series = {Journal of Pharmaceutical and Biomedical Analysis}, volume = {219}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, number = {Article number: 114915}, publisher = {Elsevier}, address = {New York, NY}, issn = {0731-7085}, doi = {10.1016/j.jpba.2022.114915}, year = {2022}, abstract = {Nuclear magnetic resonance (NMR) spectrometric methods for the quantitative analysis of pure heparin in crude heparin is proposed. For quantification, a two-step routine was developed using a USP heparin reference sample for calibration and benzoic acid as an internal standard. The method was successfully validated for its accuracy, reproducibility, and precision. The methodology was used to analyze 20 authentic porcine heparinoid samples having heparin content between 4.25 w/w \% and 64.4 w/w \%. The characterization of crude heparin products was further extended to a simultaneous analysis of these common ions: sodium, calcium, acetate and chloride. A significant, linear dependence was found between anticoagulant activity and assayed heparin content for thirteen heparinoids samples, for which reference data were available. A Diffused-ordered NMR experiment (DOSY) can be used for qualitative analysis of specific glycosaminoglycans (GAGs) in heparinoid matrices and, potentially, for quantitative prediction of molecular weight of GAGs. NMR spectrometry therefore represents a unique analytical method suitable for the simultaneous quantitative control of organic and inorganic composition of crude heparin samples (especially heparin content) as well as an estimation of other physical and quality parameters (molecular weight, animal origin and activity).}, language = {en} } @article{SelmerJennemannBaueretal.1999, author = {Selmer, Thorsten and Jennemann, Richard and Bauer, Bernhard L. and Bertalanffy, Helmut}, title = {Novel glycoinositolphosphosphingolipids, basidiolipids, from Agaricus / Jennemann, Richard ; Bauer, Bernhard, L. ; Bertalanffy, Helmut ; Geyer, Rudolf ; Gschwind, Ruth, M. ; Selmer, Thorsten ; Wiegandt, Herbert}, series = {European Journal of Biochemistry. 259 (1999), H. 1-2}, journal = {European Journal of Biochemistry. 259 (1999), H. 1-2}, isbn = {0014-2956}, pages = {331 -- 338}, year = {1999}, language = {en} } @article{BiselliJelinekSchmidtetal.2002, author = {Biselli, Manfred and Jelinek, N. and Schmidt, S. and Hilbert, U.}, title = {Novel bioreactors for the ex vivo cultivation of hematopoietic cells / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C.}, series = {Engineering in Life Sciences. 2 (2002), H. 1}, journal = {Engineering in Life Sciences. 2 (2002), H. 1}, isbn = {1618-0240}, pages = {15 -- 18}, year = {2002}, language = {en} } @article{MonakhovaDiehl2021, author = {Monakhova, Yulia and Diehl, Bernd W.K.}, title = {Novel approach of qNMR workflow by standardization using 2H integral: Application to any intrinsic calibration standard}, series = {Talanta}, volume = {222}, journal = {Talanta}, number = {Article number: 121504}, publisher = {Elsevier}, isbn = {0039-9140}, doi = {10.1016/j.talanta.2020.121504}, year = {2021}, abstract = {Quantitative nuclear magnetic resonance (qNMR) is routinely performed by the internal or external standardization. The manuscript describes a simple alternative to these common workflows by using NMR signal of another active nuclei of calibration compound. For example, for any arbitrary compound quantification by NMR can be based on the use of an indirect concentration referencing that relies on a solvent having both 1H and 2H signals. To perform high-quality quantification, the deuteration level of the utilized deuterated solvent has to be estimated. In this contribution the new method was applied to the determination of deuteration levels in different deuterated solvents (MeOD, ACN, CDCl3, acetone, benzene, DMSO-d6). Isopropanol-d6, which contains a defined number of deuterons and protons, was used for standardization. Validation characteristics (precision, accuracy, robustness) were calculated and the results showed that the method can be used in routine practice. Uncertainty budget was also evaluated. In general, this novel approach, using standardization by 2H integral, benefits from reduced sample preparation steps and uncertainties, and can be applied in different application areas (purity determination, forensics, pharmaceutical analysis, etc.).}, language = {en} } @article{HaegerProbstJaegeretal.2023, author = {Haeger, Gerrit and Probst, Johanna and Jaeger, Karl-Erich and Bongaerts, Johannes and Siegert, Petra}, title = {Novel aminoacylases from Streptomyces griseus DSM 40236 and their recombinant production in Streptomyces lividans}, series = {FEBS Open Bio}, volume = {13}, journal = {FEBS Open Bio}, number = {12}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {2211-5463}, doi = {10.1002/2211-5463.13723}, pages = {2224 -- 2238}, year = {2023}, abstract = {Amino acid-based surfactants are valuable compounds for cosmetic formulations. The chemical synthesis of acyl-amino acids is conventionally performed by the Schotten-Baumann reaction using fatty acyl chlorides, but aminoacylases have also been investigated for use in biocatalytic synthesis with free fatty acids. Aminoacylases and their properties are diverse; they belong to different peptidase families and show differences in substrate specificity and biocatalytic potential. Bacterial aminoacylases capable of synthesis have been isolated from Burkholderia, Mycolicibacterium, and Streptomyces. Although several proteases and peptidases from S. griseus have been described, no aminoacylases from this species have been identified yet. In this study, we investigated two novel enzymes produced by S. griseus DSM 40236ᵀ . We identified and cloned the respective genes and recombinantly expressed an α-aminoacylase (EC 3.5.1.14), designated SgAA, and an ε-lysine acylase (EC 3.5.1.17), designated SgELA, in S. lividans TK23. The purified aminoacylase SgAA was biochemically characterized, focusing on its hydrolytic activity to determine temperature- and pH optima and stabilities. The aminoacylase could hydrolyze various acetyl-amino acids at the Nα -position with a broad specificity regarding the sidechain. Substrates with longer acyl chains, like lauroyl-amino acids, were hydrolyzed to a lesser extent. Purified aminoacylase SgELA specific for the hydrolysis of Nε -acetyl-L-lysine was unstable and lost its enzymatic activity upon storage for a longer period but could initially be characterized. The pH optimum of SgELA was pH 8.0. While synthesis of acyl-amino acids was not observed with SgELA, SgAA catalyzed the synthesis of lauroyl-methionine.}, language = {en} } @article{LawsonScheerPhametal.2001, author = {Lawson, Nathan D. and Scheer, Nico and Pham, Van N. and Kim, Ceol-Hee and Chitnis, Ajay B. and Campos-Ortega, Jos{\´e} A. and Weinstein, Brant M.}, title = {Notch signaling is required for arterial-venous differentiation during embryonic vascular development}, series = {Development}, volume = {128}, journal = {Development}, number = {19}, issn = {1477-9129}, pages = {3675 -- 3683}, year = {2001}, language = {en} } @article{KotterGregerIhme1984, author = {Kotter, Michael and Greger, M. and Ihme, B.}, title = {Nonequilibrium phase transitions with hysteresis in solid catalysts : the system copper-oxygen-propene / M. Greger ; B. Ihme ; M. Kotter ...}, series = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 88 (1984)}, journal = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 88 (1984)}, isbn = {0005-9021}, pages = {427 -- 433}, year = {1984}, language = {en} } @article{BaumannSemenehDehairsetal.1998, author = {Baumann, Marcus and Semeneh, M. and Dehairs, F. and Elskens, M.}, title = {Nitrogen uptake regime and phytoplankton community structure in the Southern Ocean / Semeneh, M. ; Dehairs, F. ; Elskens, M. ; Baumann, M.E.M. ; Kopczynska, E.E. ; Lancelot, C. ; Goeyens, L.}, series = {Journal of Marine Systems. 17 (1998), H. 1-4}, journal = {Journal of Marine Systems. 17 (1998), H. 1-4}, isbn = {0924-7963}, pages = {159 -- 177}, year = {1998}, language = {en} } @article{BrabandPaulssenAbram2006, author = {Braband, Henrik and Paulßen, Elisabeth and Abram, Ulrich}, title = {Nitridorhenium(V) Complexes with 1,3-Dialkyl-4,5-dimethylimidazole-2-ylidenes}, series = {Zeitschrift f{\"u}r anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry}, volume = {632}, journal = {Zeitschrift f{\"u}r anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry}, number = {6}, issn = {1521-3749}, doi = {10.1002/zaac.200600002}, pages = {1051 -- 1056}, year = {2006}, language = {en} } @article{TippkoetterRoikaewUlber2008, author = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, R.}, title = {Nitrate removal from whey concentrate with biotechnological regeneration of the waste water}, series = {European dairy magazine : EDM}, journal = {European dairy magazine : EDM}, number = {1}, isbn = {0936-6318}, pages = {30 -- 32}, year = {2008}, language = {en} } @article{ElbersGaebHoffmannetal.2006, author = {Elbers, Gereon and G{\"a}b, Martina and Hoffmann, Katharina and Lobe, Melanie}, title = {NIR-spectroscopic investigation of foliage of ozone-stressed Fagus sylvatica trees / G{\"a}b, Martina ; Hoffmann, Katharina ; Lobe, Melanie ; Metzger, Rut ; Ooyen, Sven van ; Elbers, Gereon ; K{\"o}llner, Barbara}, series = {Journal of Forest Research. 11 (2006), H. 2}, journal = {Journal of Forest Research. 11 (2006), H. 2}, isbn = {1341-6979}, pages = {69 -- 75}, year = {2006}, language = {en} } @article{ThielTippkoetterSucketal.2013, author = {Thiel, Alexander and Tippk{\"o}tter, Nils and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland}, title = {New zeolite adsorbents for downstream processing of polyphenols from renewable resources}, series = {Engineering in Life Sciences}, volume = {13}, journal = {Engineering in Life Sciences}, number = {3}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/elsc.201200188}, pages = {239 -- 246}, year = {2013}, abstract = {Commercial materials with polyvinylpolypyrrolidone and polymeric amberlites (XAD7HP, XAD16) are commonly used for the adsorptive downstream processing of polyphenols from renewable resources. In this study, beta-zeolite-based adsorbent systems were examined, and their properties were compared to organic resins. Batch adsorption experiments were conducted with synthetic solutions of major polyphenols. Adsorption isotherms and desorption characteristics of individual adsorbent were determined based on these results. Maximum adsorption capacities were calculated using the Langmuir model. For example, the zeolites had capacities up to 203.2 mg/g for ferulic acid. To extend these results to a complex system, additional experiments were performed on rapeseed meal and wheat seed extracts as representative renewable resources. HPLC analysis showed that with 7.5\% w/v, which is regarded as the optimum amount of zeolites, zeolites A and B could bind 100\% of the major polyphenols as well as release polyphenols at high yields. Additionally, regeneration experiments were performed with isopropyl alcohol at 99°C to evaluate how zeolites regenerate under mild conditions. The results showed only a negligible loss of adsorption capacity and no loss of desorption capacity. In summary, it was concluded that beta-zeolites were promising adsorbents for developing new processes to isolate polyphenols from renewable resources.}, language = {en} } @article{FalkenbergVossBottetal.2023, author = {Falkenberg, Fabian and Voß, Leonie and Bott, Michael and Bongaerts, Johannes and Siegert, Petra}, title = {New robust subtilisins from halotolerant and halophilic Bacillaceae}, series = {Applied Microbiology and Biotechnology}, volume = {107}, journal = {Applied Microbiology and Biotechnology}, publisher = {Springer Nature}, address = {Berlin}, issn = {1432-0614}, doi = {10.1007/s00253-023-12553-w}, pages = {3939 -- 3954}, year = {2023}, abstract = {The aim of the present study was the characterisation of three true subtilisins and one phylogenetically intermediate subtilisin from halotolerant and halophilic microorganisms. Considering the currently growing enzyme market for efficient and novel biocatalysts, data mining is a promising source for novel, as yet uncharacterised enzymes, especially from halophilic or halotolerant Bacillaceae, which offer great potential to meet industrial needs. Both halophilic bacteria Pontibacillus marinus DSM 16465ᵀ and Alkalibacillus haloalkaliphilus DSM 5271ᵀ and both halotolerant bacteria Metabacillus indicus DSM 16189 and Litchfieldia alkalitelluris DSM 16976ᵀ served as a source for the four new subtilisins SPPM, SPAH, SPMI and SPLA. The protease genes were cloned and expressed in Bacillus subtilis DB104. Purification to apparent homogeneity was achieved by ethanol precipitation, desalting and ion-exchange chromatography. Enzyme activity could be observed between pH 5.0-12.0 with an optimum for SPPM, SPMI and SPLA around pH 9.0 and for SPAH at pH 10.0. The optimal temperature for SPMI and SPLA was 70 °C and for SPPM and SPAH 55 °C and 50 °C, respectively. All proteases showed high stability towards 5\% (w/v) SDS and were active even at NaCl concentrations of 5 M. The four proteases demonstrate potential for future biotechnological applications.}, language = {en} } @article{SchererKratzGoberetal.1992, author = {Scherer, Ulrich W. and Kratz, J. V. and Gober, M. K. and Zimmermann, H. P.}, title = {New nuclide 263 105 / J.V. Kratz, M.K. Gober, H.P. Zimmermann, M. Sch{\"a}del, W. Br{\"u}chle, E. Schimpf, K.E. Gregorich, A. T{\"u}rler, N.J. Hannink, K.R. Czerwinski, B. Kadkhodayan, D.M. Lee, M.J. Nurmia, D.C. Hoffman, H. G{\"a}ggeler, D. Jost, U.W. Scherer, A. Weber}, series = {Physical Review C . 45 (1992)}, journal = {Physical Review C . 45 (1992)}, pages = {1064 -- 1069}, year = {1992}, language = {en} } @article{SelmerPierikHeider2005, author = {Selmer, Thorsten and Pierik, Antonio J. and Heider, Johann}, title = {New glycyl radical enzymes catalysing key metabolic steps in anaerobic bacteria}, series = {Biological Chemistry. 386 (2005), H. 10}, journal = {Biological Chemistry. 386 (2005), H. 10}, isbn = {1431-6730}, pages = {981 -- 988}, year = {2005}, language = {en} } @article{DruckenmuellerGuentherElbers2018, author = {Druckenm{\"u}ller, Katharina and G{\"u}nther, Klaus and Elbers, Gereon}, title = {Near-infrared spectroscopy (NIRS) as a tool to monitor exhaust air from poultry operations}, series = {Science of the Total Environment}, volume = {630}, journal = {Science of the Total Environment}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0048-9697}, doi = {10.1016/j.scitotenv.2018.02.072}, pages = {536 -- 543}, year = {2018}, abstract = {Intensive poultry operation systems emit a considerable volume of inorganic and organic matter in the surrounding environment. Monitoring cleaning properties of exhaust air cleaning systems and to detect small but significant changes in emission characteristics during a fattening cycle is important for both emission and fattening process control. In the present study, we evaluated the potential of near-infrared spectroscopy (NIRS) combined with chemometric techniques as a monitoring tool of exhaust air from poultry operation systems. To generate a high-quality data set for evaluation, the exhaust air of two poultry houses was sampled by applying state-of-the-art filter sampling protocols. The two stables were identical except for one crucial difference, the presence or absence of an exhaust air cleaning system. In total, twenty-one exhaust air samples were collected at the two sites to monitor spectral differences caused by the cleaning device, and to follow changes in exhaust air characteristics during a fattening period. The total dust load was analyzed by gravimetric determination and included as a response variable in multivariate data analysis. The filter samples were directly measured with NIR spectroscopy. Principal component analysis (PCA), linear discriminant analysis (LDA), and factor analysis (FA) were effective in classifying the NIR exhaust air spectra according to fattening day and origin. The results indicate that the dust load and the composition of exhaust air (inorganic or organic matter) substantially influence the NIR spectral patterns. In conclusion, NIR spectroscopy as a tool is a promising and very rapid way to detect differences between exhaust air samples based on still not clearly defined circumstances triggered during a fattening period and the availability of an exhaust air cleaning system.}, language = {en} } @article{SvaneborgKarimiVarzanehHojdisetal.2016, author = {Svaneborg, Carsten and Karimi-Varzaneh, Hossein Ali and Hojdis, Nils and Fleck, Franz and Everaers, Ralf}, title = {Multiscale approach to equilibrating model polymer melts}, series = {Physical Review E}, volume = {94}, journal = {Physical Review E}, number = {032502}, publisher = {AIP Publishing}, address = {Melville, NY}, issn = {2470-0053}, doi = {10.1103/PhysRevE.94.032502}, year = {2016}, abstract = {We present an effective and simple multiscale method for equilibrating Kremer Grest model polymer melts of varying stiffness. In our approach, we progressively equilibrate the melt structure above the tube scale, inside the tube and finally at the monomeric scale. We make use of models designed to be computationally effective at each scale. Density fluctuations in the melt structure above the tube scale are minimized through a Monte Carlo simulated annealing of a lattice polymer model. Subsequently the melt structure below the tube scale is equilibrated via the Rouse dynamics of a force-capped Kremer-Grest model that allows chains to partially interpenetrate. Finally the Kremer-Grest force field is introduced to freeze the topological state and enforce correct monomer packing. We generate 15 melts of 500 chains of 10.000 beads for varying chain stiffness as well as a number of melts with 1.000 chains of 15.000 monomers. To validate the equilibration process we study the time evolution of bulk, collective, and single-chain observables at the monomeric, mesoscopic, and macroscopic length scales. Extension of the present method to longer, branched, or polydisperse chains, and/or larger system sizes is straightforward.}, language = {en} } @article{PrielmeierLang1988, author = {Prielmeier, Franz and Lang, E. W.}, title = {Multinuclear Spin-Lattice Relaxation Time Studies of Supercooled Aqueous LiCl-Solutions / E .W. Lang, F. X. Prielmeier}, series = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 92 (1988)}, journal = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 92 (1988)}, isbn = {0005-9021}, pages = {717}, year = {1988}, language = {en} } @article{MonakhovaDiehl2022, author = {Monakhova, Yulia and Diehl, Bernd W.K.}, title = {Multinuclear NMR screening of pharmaceuticals using standardization by 2H integral of a deuterated solvent}, series = {Journal of Pharmaceutical and Biomedical Analysis}, volume = {209}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, number = {Article number: 114530}, publisher = {Elsevier}, isbn = {0731-7085}, doi = {10.1016/j.jpba.2021.114530}, year = {2022}, abstract = {NMR standardization approach that uses the 2H integral of deuterated solvent for quantitative multinuclear analysis of pharmaceuticals is described. As a proof of principle, the existing NMR procedure for the analysis of heparin products according to US Pharmacopeia monograph is extended to the determination of Na+ and Cl- content in this matrix. Quantification is performed based on the ratio of a 23Na (35Cl) NMR integral and 2H NMR signal of deuterated solvent, D2O, acquired using the specific spectrometer hardware. As an alternative, the possibility of 133Cs standardization using the addition of Cs2CO3 stock solution is shown. Validation characteristics (linearity, repeatability, sensitivity) are evaluated. A holistic NMR profiling of heparin products can now also be used for the quantitative determination of inorganic compounds in a single analytical run using a single sample. In general, the new standardization methodology provides an appealing alternative for the NMR screening of inorganic and organic components in pharmaceutical products.}, language = {en} } @article{AdelsElbersDiehletal.2023, author = {Adels, Klaudia and Elbers, Gereon and Diehl, Bernd and Monakhova, Yulia}, title = {Multicomponent analysis of dietary supplements containing glucosamine and chondroitin: comparative low- and high-field NMR spectroscopic study}, series = {Analytical Sciences}, volume = {2023}, journal = {Analytical Sciences}, publisher = {Springer Verlag}, address = {Cham}, issn = {1348-2246 (Online)}, doi = {10.1007/s44211-023-00433-2}, pages = {13 Seiten}, year = {2023}, abstract = {With the prevalence of glucosamine- and chondroitin-containing dietary supplements for people with osteoarthritis in the marketplace, it is important to have an accurate and reproducible analytical method for the quantitation of these compounds in finished products. NMR spectroscopic method based both on low- (80 MHz) and high- (500-600 MHz) field NMR instrumentation was established, compared and validated for the determination of chondroitin sulfate and glucosamine in dietary supplements. The proposed method was applied for analysis of 20 different dietary supplements. In the majority of cases, quantification results obtained on the low-field NMR spectrometer are similar to those obtained with high-field 500-600 MHz NMR devices. Validation results in terms of accuracy, precision, reproducibility, limit of detection and recovery demonstrated that the developed method is fit for purpose for the marketed products. The NMR method was extended to the analysis of methylsulfonylmethane, adulterant maltodextrin, acetate and inorganic ions. Low-field NMR can be a quicker and cheaper alternative to more expensive high-field NMR measurements for quality control of the investigated dietary supplements. High-field NMR instrumentation can be more favorable for samples with complex composition due to better resolution, simultaneously giving the possibility of analysis of inorganic species such as potassium and chloride.}, language = {en} } @article{MangHaulena1992, author = {Mang, Thomas and Haulena, Friedhelm}, title = {Monodisperse latex particles with functional groups}, series = {Modification of polymers : 4th Dresden Polymer Discussion ; September 22 to 25, 1992 at Gaussig / Dresden University of Technology, Department of Macromolecular Chemistry and Textile Chemistry; Institute of Polymer Technology Dresden. [Organizers: Claudia Kummerl{\"o}we ...]}, journal = {Modification of polymers : 4th Dresden Polymer Discussion ; September 22 to 25, 1992 at Gaussig / Dresden University of Technology, Department of Macromolecular Chemistry and Textile Chemistry; Institute of Polymer Technology Dresden. [Organizers: Claudia Kummerl{\"o}we ...]}, publisher = {Univ.}, address = {Dresden}, pages = {68 -- 88}, year = {1992}, language = {en} } @article{MeyerHentschkeHageretal.2017, author = {Meyer, Jan and Hentschke, Reinhard and Hager, Jonathan and Hojdis, Nils and Karimi-Varzaneh, Hossein Ali}, title = {Molecular Simulation of Viscous Dissipation due to Cyclic Deformation of a Silica-Silica Contact in Filled Rubber}, series = {Macromolecules}, volume = {50}, journal = {Macromolecules}, number = {17}, issn = {1520-5835}, doi = {10.1021/acs.macromol.7b00947}, pages = {6679 -- 6689}, year = {2017}, language = {en} } @article{LauthHoelderichWagenblast1995, author = {Lauth, Jakob and Hoelderich, W. and Wagenblast, G.}, title = {Molecular sieves containing azo dyes}, series = {Zeolites. 15 (1995), H. 2}, journal = {Zeolites. 15 (1995), H. 2}, isbn = {0144-2449}, pages = {184}, year = {1995}, language = {en} } @article{HentschkeHagerHojdis2014, author = {Hentschke, Reinhard and Hager, Jonathan and Hojdis, Nils}, title = {Molecular Modeling Approach to the Prediction of Mechanical Properties of Silica-Reinforced Rubbers}, series = {Journal of Applied Polymer Science}, volume = {131}, journal = {Journal of Applied Polymer Science}, number = {18}, publisher = {Wiley}, address = {New York, NY}, issn = {1097-4628}, doi = {10.1002/app.40806}, pages = {1 -- 9}, year = {2014}, abstract = {Recently, we have suggested a nanomechanical model for dissipative loss in filled elastomer networks in the context of the Payne effect. The mechanism is based on a total interfiller particle force exhibiting an intermittent loop, due to the combination of short-range repulsion and dispersion forces with a long-range elastic attraction. The sum of these forces leads, under external strain, to a spontaneous instability of "bonds" between the aggregates in a filler network and attendant energy dissipation. Here, we use molecular dynamics simulations to obtain chemically realistic forces between surface modified silica particles. The latter are combined with the above model to estimate the loss modulus and the low strain storage modulus in elastomers containing the aforementioned filler-compatibilizer systems. The model is compared to experimental dynamic moduli of silica filled rubbers. We find good agreement between the model predictions and the experiments as function of the compatibilizer's molecular structure and its bulk concentration.}, language = {en} } @article{DuttaHartkopfFroederWitteetal.2013, author = {Dutta, Suryendu and Hartkopf-Fr{\"o}der, Christoph and Witte, Karin and Brocke, Rainer and Mann, Ulrich}, title = {Molecular characterization of fossil palynomorphs by transmission micro-FTIR spectroscopy: implications for hydrocarbon source evaluation}, series = {International journal of coal geology}, volume = {Vol. 115}, journal = {International journal of coal geology}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1872-7840 (E-Journal); 0166-5162 (Print)}, pages = {13 -- 23}, year = {2013}, language = {en} } @article{WerkhausenAlbrachtCroninetal.2017, author = {Werkhausen, Amelie and Albracht, Kirsten and Cronin, Neil J. and Meier, Rahel and Mojsen-Moeller, Jens and Seynnes, Olivier R.}, title = {Modulation of muscle-tendon interaction in the human triceps surae during an energy dissipation task}, series = {Journal of Experimental Biology}, volume = {220}, journal = {Journal of Experimental Biology}, number = {22}, issn = {0022-0949}, doi = {10.1242/jeb.164111}, pages = {4141 -- 4149}, year = {2017}, language = {en} } @article{OehlschlaegerSteinbergSehretal.2005, author = {{\"O}hlschl{\"a}ger, Peter and Steinberg, Thorsten and Sehr, Peter and Osen, Wolfram}, title = {Modification of HPV 16 E7 genes: correlation between the level of protein expression and CTL response after immunization of C57BL/6 mice / Steinberg, Thorsten ; {\"O}hlschl{\"a}ger, Peter ; Sehr, Peter ; Osen, Wolfram ; Gissmann, Lutz}, series = {Vaccine. 23 (2005), H. 9}, journal = {Vaccine. 23 (2005), H. 9}, isbn = {0264-410X}, pages = {1149 -- 1157}, year = {2005}, language = {en} } @article{MangKricheldorfAu1985, author = {Mang, Thomas and Kricheldorf, Hans R. and Au, M.}, title = {Models of molecular evolution. 2. Stereospecificity of dipeptide syntheses by means of cyanamides and carbodiimides / Kricheldorf, Hans R. ; Au, M. ; Mang, Thomas}, series = {International journal of peptide and protein research. 26 (1985), H. 2}, journal = {International journal of peptide and protein research. 26 (1985), H. 2}, isbn = {0300-9769}, pages = {149 -- 157}, year = {1985}, language = {en} } @article{ScheerKapelyukhMcEwanetal.2012, author = {Scheer, Nico and Kapelyukh, Yury and McEwan, Jillian and Beuger, Vincent and Stanley, Lesley A. and Rode, Anja and Wolf, C. Roland}, title = {Modeling Human Cytochrome P450 2D6 Metabolism and Drug-drug Interaction by a Novel Panel of Knockout and Humanized Mouse Lines}, series = {Molecular Pharmacology}, volume = {81}, journal = {Molecular Pharmacology}, number = {1}, publisher = {ASPET}, address = {Bethesda, Md.}, issn = {1521-0111}, doi = {10.1124/mol.111.075192}, pages = {63 -- 72}, year = {2012}, abstract = {The highly polymorphic human cytochrome P450 2D6 enzyme is involved in the metabolism of up to 25\% of all marketed drugs and accounts for significant individual differences in response to CYP2D6 substrates. Because of the differences in the multiplicity and substrate specificity of CYP2D family members among species, it is difficult to predict pathways of human CYP2D6-dependent drug metabolism on the basis of animal studies. To create animal models that reflect the human situation more closely and that allow an in vivo assessment of the consequences of differential CYP2D6 drug metabolism, we have developed a novel straightforward approach to delete the entire murine Cyp2d gene cluster and replace it with allelic variants of human CYP2D6. By using this approach, we have generated mouse lines expressing the two frequent human protein isoforms CYP2D6.1 and CYP2D6.2 and an as yet undescribed variant of this enzyme, as well as a Cyp2d cluster knockout mouse. We demonstrate that the various transgenic mouse lines cover a wide spectrum of different human CYP2D6 metabolizer phenotypes. The novel humanization strategy described here provides a robust approach for the expression of different CYP2D6 allelic variants in transgenic mice and thus can help to evaluate potential CYP2D6-dependent interindividual differences in drug response in the context of personalized medicine.}, language = {en} } @article{BaeckerRaueSchusseretal.2012, author = {B{\"a}cker, Matthias and Raue, Markus and Schusser, Sebastian and Jeitner, C. and Breuer, L. and Wagner, P. and Poghossian, Arshak and F{\"o}rster, Arnold and Mang, Thomas and Sch{\"o}ning, Michael Josef}, title = {Microfluidic chip with integrated microvalves based on temperature- and pH-responsive hydrogel thin films}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100763}, pages = {839 -- 845}, year = {2012}, abstract = {Two types of microvalves based on temperature-responsive poly(N-isopropylacrylamide) (PNIPAAm) and pH-responsive poly(sodium acrylate) (PSA) hydrogel films have been developed and tested. The PNIPAAm and PSA hydrogel films were prepared by means of in situ photopolymerization directly inside the fluidic channel of a microfluidic chip fabricated by combining Si and SU-8 technologies. The swelling/shrinking properties and height changes of the PNIPAAm and PSA films inside the fluidic channel were studied at temperatures of deionized water from 14 to 36 °C and different pH values (pH 3-12) of Titrisol buffer, respectively. Additionally, in separate experiments, the lower critical solution temperature (LCST) of the PNIPAAm hydrogel was investigated by means of a differential scanning calorimetry (DSC) and a surface plasmon resonance (SPR) method. Mass-flow measurements have shown the feasibility of the prepared hydrogel films to work as an on-chip integrated temperature- or pH-responsive microvalve capable to switch the flow channel on/off.}, language = {en} } @article{SelmerPinkenburg2008, author = {Selmer, Thorsten and Pinkenburg, Olaf}, title = {Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases / Selmer, Thorsten ; Pinkenburg, Olaf}, year = {2008}, language = {en} } @article{WilmingBegemannKuhneetal.2013, author = {Wilming, Anja and Begemann, Jens and Kuhne, Stefan and Regestein, Lars and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and B{\"u}chs, Jochen}, title = {Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations}, series = {Biochemical engineering journal}, volume = {Vol. 73}, journal = {Biochemical engineering journal}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-295X (E-Journal); 1369-703X (Print)}, pages = {29 -- 37}, year = {2013}, language = {en} } @article{HuckSchiffelsHerreraetal.2013, author = {Huck, Christina and Schiffels, Johannes and Herrera, Cony N. and Schelden, Maximilian and Selmer, Thorsten and Poghossian, Arshak and Baumann, Marcus and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Metabolic responses of Escherichia coli upon glucose pulses captured by a capacitive field-effect sensor}, series = {Physica Status Solidi (A)}, volume = {210}, journal = {Physica Status Solidi (A)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0031-8965}, doi = {10.1002/pssa.201200900}, pages = {926 -- 931}, year = {2013}, abstract = {Living cells are complex biological systems transforming metabolites taken up from the surrounding medium. Monitoring the responses of such cells to certain substrate concentrations is a challenging task and offers possibilities to gain insight into the vitality of a community influenced by the growth environment. Cell-based sensors represent a promising platform for monitoring the metabolic activity and thus, the "welfare" of relevant organisms. In the present study, metabolic responses of the model bacterium Escherichia coli in suspension, layered onto a capacitive field-effect structure, were examined to pulses of glucose in the concentration range between 0.05 and 2 mM. It was found that acidification of the surrounding medium takes place immediately after glucose addition and follows Michaelis-Menten kinetic behavior as a function of the glucose concentration. In future, the presented setup can, therefore, be used to study substrate specificities on the enzymatic level and may as well be used to perform investigations of more complex metabolic responses. Conclusions and perspectives highlighting this system are discussed.}, language = {en} } @article{MuellerBongaertsBovenbergetal.2001, author = {M{\"u}ller, Ulrike and Bongaerts, Johannes and Bovenberg, Roel and Jossek, Ralf and Kr{\"a}mer, Marco and Linnemann, J. and M{\"u}schen, S. and Ritterbecks, S. and Sprenger, G. and Wubbolts, Marcel}, title = {Metabolic engineering to produce fine chemicals in Escherichia coli}, series = {Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent}, volume = {66 (3a)}, journal = {Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent}, issn = {0035-533x}, pages = {215 -- 217}, year = {2001}, language = {en} } @article{BongaertsBovenbergKraemeretal.2002, author = {Bongaerts, Johannes and Bovenberg, Roel and Kr{\"a}mer, Marco and M{\"u}ller, Ulrike and Raeven, Leon and Wubbolts, Marcel}, title = {Metabolic engineering to produce fine chemicals in Escherichia coli}, series = {Chemie - Ingenieur - Technik (CIT)}, volume = {Vol. 74}, journal = {Chemie - Ingenieur - Technik (CIT)}, number = {Iss. 5}, issn = {1522-2640 (E-Journal); 0009-286X (Print)}, pages = {694}, year = {2002}, language = {en} } @article{KraemerBongaertsBovenbergetal.2003, author = {Kr{\"a}mer, Marco and Bongaerts, Johannes and Bovenberg, Roel and Kremer, Susanne and M{\"u}ller, Ulrike and Orf, Sonja and Wubbolts, Marcel and Raeven, Leon}, title = {Metabolic engineering for microbial production of shikimic acid}, series = {Metabolic engineering}, volume = {Vol. 5}, journal = {Metabolic engineering}, number = {Iss. 4}, issn = {1096-7184 (E-Journal); 1096-7176 (Print)}, pages = {277 -- 283}, year = {2003}, language = {en} } @article{BongaertsKraemerMuelleretal.2001, author = {Bongaerts, Johannes and Kr{\"a}mer, Marco and M{\"u}ller, Ulrike and Raeven, Leon and Wubbolts, Marcel}, title = {Metabolic engineering for microbial production of aromatic amino acids and derived compounds}, series = {Metabolic engineering}, volume = {Vol. 3}, journal = {Metabolic engineering}, number = {Iss. 4}, issn = {1096-7184 (E-Journal); 1096-7176 (Print)}, pages = {289 -- 300}, year = {2001}, language = {en} }