@article{BiselliNollMuehlsiepenetal.2000, author = {Biselli, Manfred and Noll, Thomas and M{\"u}hlsiepen, Heinz and Engels, Ralf}, title = {A cell-culture reactor for the on-line evaluation of radiopharmaceuticals : evaluation of the lumped constant of FDG in human glioma cells / Noll, Thomas ; M{\"u}hlensiepen, Heinz ; Engels, Ralf ; Hamacher, Kurt ; Papaspyrou, Manfred ; Langen, Karl-Josef ; Bi}, series = {Journal of Nuclear Medicine. 41 (2000), H. 3}, journal = {Journal of Nuclear Medicine. 41 (2000), H. 3}, isbn = {0022-3123}, pages = {556 -- 564}, year = {2000}, language = {en} } @article{BiselliHilbertJelineketal.2001, author = {Biselli, Manfred and Hilbert, U. and Jelinek, N. and Schmidt, S.}, title = {Bioprocess development for the cultivation of human T-lymphocytes / Hilbert, U. ; Jelinek, N. Schmidt, S. ; Biselli, M.}, series = {Engineering in Life Sciences. 1 (2001)}, journal = {Engineering in Life Sciences. 1 (2001)}, isbn = {1618-0240}, pages = {20 -- 23}, year = {2001}, language = {en} } @article{BiselliNollEisenkraetzeretal.2002, author = {Biselli, Manfred and Noll, Thomas and Eisenkr{\"a}tzer, Detlef and Kiesewetter, Andr{\´e}}, title = {Improved product formation in high density Chinese hamster ovary cell cultures transfected at confluency / Thomas Noll, Detlef Eisenkr{\"a}tzer, Andr{\´e} Kiesewetter, Andr{\´e} Dinter, Steffen Zeng, Christian Wandrey, Manfred Biselli und Eric G. Berger}, series = {Biotechnology Letters. 24 (2002), H. 11}, journal = {Biotechnology Letters. 24 (2002), H. 11}, isbn = {0141-5492}, pages = {861 -- 866}, year = {2002}, language = {en} } @article{BiselliJelinekSchmidtetal.2002, author = {Biselli, Manfred and Jelinek, N. and Schmidt, S. and Hilbert, U.}, title = {Novel bioreactors for the ex vivo cultivation of hematopoietic cells / Jelinek, N. ; Schmidt, S. ; Hilbert, U. ; Thoma, S. ; Biselli, M. ; Wandrey, C.}, series = {Engineering in Life Sciences. 2 (2002), H. 1}, journal = {Engineering in Life Sciences. 2 (2002), H. 1}, isbn = {1618-0240}, pages = {15 -- 18}, year = {2002}, language = {en} } @article{BiselliNollJelineketal.2002, author = {Biselli, Manfred and Noll, Thomas and Jelinek, Nanni and Schmidt, Sebastian}, title = {Cultivation of Hematopoietic Stem and Progenitor Cells: biochemical Engineering Aspects / Thomas Noll, Nanni Jelinek, Sebastian Schmidt, Manfred Biselli und Christian Wandrey}, series = {Tools and Applications of Biochemical Engineering Science}, journal = {Tools and Applications of Biochemical Engineering Science}, publisher = {Springer}, address = {Berlin}, isbn = {3-540-42250-1}, pages = {111 -- 128}, year = {2002}, language = {en} } @article{MaggakisKelemenBorkKayseretal.2003, author = {Maggakis-Kelemen, C. and Bork, M. and Kayser, Peter and Biselli, Manfred and Artmann, Gerhard}, title = {Biological and mechanical quality of red blood cells cultured from human umbilical cord blood stem cells}, series = {Medical and biological engineering and computing. 41 (2003), H. 3}, journal = {Medical and biological engineering and computing. 41 (2003), H. 3}, isbn = {0140-0118}, pages = {350 -- 356}, year = {2003}, language = {en} } @article{BiselliRechmannFriedrichetal.2007, author = {Biselli, Manfred and Rechmann, Hanrik and Friedrich, Andrea and Forouzan, Dara}, title = {Characterization of photosynthetically active duckweed ( Wolffia australiana ) in vitro culture by Respiration Activity Monitoring System (RAMOS) / Henrik Rechmann, Andrea Friedrich, Dara Forouzan, Stefan Barth, Heide Schnabl, Manfred Biselli und Robert B}, series = {Biotechnology Letters. 29 (2007), H. 6}, journal = {Biotechnology Letters. 29 (2007), H. 6}, isbn = {0141-5492}, pages = {971 -- 977}, year = {2007}, language = {en} } @article{BiselliHambachRunstadleretal.1992, author = {Biselli, Manfred and Hambach, B. and Runstadler, P. W. and Wandrey, Christian}, title = {Development of a reactor-integrated aeration system for cultivation of animal cells in fluidized beds / Hambach, B. ; Biselli, M. ; Runstadler, P.W. ; Wandrey, C.}, series = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750604212}, pages = {381 -- 385}, year = {1992}, language = {en} } @article{BiselliLuellauDreisbachetal.1992, author = {Biselli, Manfred and L{\"u}llau, E. and Dreisbach, C. and Grogg, A.}, title = {Immobilization of animal cells on chemically modified siran carrier / L{\"u}llau, E. ; Dreisbach, C. ; Grogg, A. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750604212}, pages = {469 -- 475}, year = {1992}, language = {en} } @article{BiselliVanderPolJokschetal.1992, author = {Biselli, Manfred and Van der Pol, Jens J. and Joksch, B. and Eberhardt, R.}, title = {Development of an enzymatic multichannel flow injection analysis system for monitoring mammalian cell fermentations / Van der Pol, J.J.; Joksch, B.; Eberhardt, R.; Biselli, M.; Wandrey, C., Tramper, J.}, series = {Biosensors : fundamentals, technologies and applications ; contributions to the BMFT status seminar with international participation May, 12 to 14, 1991, Internationales Bildungs-Centrum Bogensee/Brandenburg, Germany / org. by ZIM-Zentralinstitut f{\"u}r Molekularbiologie, Berlin-Buch and GBF, Braunschweig. Ed. by F. Scheller}, journal = {Biosensors : fundamentals, technologies and applications ; contributions to the BMFT status seminar with international participation May, 12 to 14, 1991, Internationales Bildungs-Centrum Bogensee/Brandenburg, Germany / org. by ZIM-Zentralinstitut f{\"u}r Molekularbiologie, Berlin-Buch and GBF, Braunschweig. Ed. by F. Scheller}, publisher = {VCH}, address = {Weinheim}, isbn = {3527284370}, pages = {349 -- 352}, year = {1992}, language = {en} } @article{BiselliThoemmesGaetgensetal.1993, author = {Biselli, Manfred and Th{\"o}mmes, J. and G{\"a}tgens, J. and Runstadler, P. W.}, title = {The influence of dissolved oxygen tension on the metabolic activity of an immobilized hybridoma population / Th{\"o}mmes, J. ; G{\"a}tgens, J. ; Biselli, M. ; Runstadler, P.W. ; Wandrey, C.}, series = {Cytotechnology . 13 (1993), H. 1}, journal = {Cytotechnology . 13 (1993), H. 1}, isbn = {0920-9069}, pages = {29 -- 39}, year = {1993}, language = {en} } @article{BiselliLuellauWandrey1994, author = {Biselli, Manfred and L{\"u}llau, E. and Wandrey, Christian}, title = {Growth and metabolism of CHO-cells in porous glass carriers / L{\"u}llau, E. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750618450}, pages = {252 -- 255}, year = {1994}, language = {en} } @article{BiselliRolefDunkeretal.1994, author = {Biselli, Manfred and Rolef, G. and Dunker, R. and Wandrey, Christian}, title = {Optimization of antibody production in a fluidized bed bioreactor / Rolef, G. ; Biselli, M. ; Dunker, R. ; Wandrey, C.}, series = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750618450}, pages = {481 -- 484}, year = {1994}, language = {en} } @article{BiselliThoemmesKulaetal.1994, author = {Biselli, Manfred and Th{\"o}mmes, J. and Kula, M.-R. and Wandrey, Christian}, title = {Batch kinetic data of hybridoma growth and productivity as a basis for the simulation of antibody production in various culture systems / Th{\"o}mmes, J. ; Biselli, M. ; Kula, M.-R. ; Wandrey, C.}, series = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750618450}, pages = {513 -- 517}, year = {1994}, language = {en} } @article{PrielmeierLangSpeedyetal.1987, author = {Prielmeier, Franz and Lang, E. W. and Speedy, R. J. and L{\"u}demann, H.-D.}, title = {Diffusion in supercooled water to 300 MPa}, series = {Physical Review Letters. 59 (1987), H. 10}, journal = {Physical Review Letters. 59 (1987), H. 10}, isbn = {0031-9007}, pages = {1128 -- 1131}, year = {1987}, language = {en} } @article{PrielmeierRadkowitschLangetal.1986, author = {Prielmeier, Franz and Radkowitsch, H. and Lang, E. W. and L{\"u}demann, H.-D.}, title = {Density dependence of the molecular dynamics of fluid CH3F and CF3H studied by NMR / H. Radkowitsch, F. X. Prielmeier, E. W. Lang and H.-D. L{\"u}demann}, series = {Physica B+C. 139-140 (1986)}, journal = {Physica B+C. 139-140 (1986)}, isbn = {0921-4526}, pages = {96 -- 99}, year = {1986}, language = {en} } @article{PrielmeierWoznyjLuedemann1984, author = {Prielmeier, Franz and Woznyj, M. and L{\"u}demann, H.-D.}, title = {Pressure Dependence of the Melting and Self Diffusion in 2,2-Dimethylpropane, 2,2-Dimethylpropionitrile, and 2-Methylpropanol-2 / M. Woznyj, F. X. Prielmeier, H.-D. L{\"u}demann}, series = {Zeitschrift f{\"u}r Naturforschung A, Journal of Physical Sciences. 39 (1984)}, journal = {Zeitschrift f{\"u}r Naturforschung A, Journal of Physical Sciences. 39 (1984)}, isbn = {0932-0784}, pages = {800}, year = {1984}, language = {en} } @article{PrielmeierSpeedyLuedemann1987, author = {Prielmeier, Franz and Speedy, R. J. and L{\"u}demann, H.-D.}, title = {High Pressure NMR Self-Diffusion Studies on Supercooled Water}, series = {High Pressure Science and Technology Proceeding XI AIRAPT, Kiew. 1}, journal = {High Pressure Science and Technology Proceeding XI AIRAPT, Kiew. 1}, pages = {75}, year = {1987}, language = {en} } @article{BiselliVanderPolMachniketal.1997, author = {Biselli, Manfred and Van der Pol, Jens J. and Machnik, Marc and Portela-Klein, Theresa}, title = {On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells / Jens J. van der Pol, Marc Machnik, Manfred Biselli, Theresa Portela-Klein, Cornelis D. de Gooijer, Johannes Tramper und Christian Wandrey}, series = {Cytotechnology. 24 (1997), H. 1}, journal = {Cytotechnology. 24 (1997), H. 1}, isbn = {0920-9069}, pages = {19 -- 30}, year = {1997}, language = {en} } @article{BiselliMeissnerSchroederetal.1999, author = {Biselli, Manfred and Meissner, Petra and Schr{\"o}der, Bernd and Herfurth, Cornelia}, title = {Development of a fixed bed bioreactor for the expansion of human hematopoietic progenitor cells / Meissner, Petra ; Schr{\"o}der, Bernd ; Herfurth, Cornelia ; Biselli, Manfred}, series = {Cytotechnology. 30 (1999), H. 1}, journal = {Cytotechnology. 30 (1999), H. 1}, isbn = {0920-9069}, pages = {227 -- 234}, year = {1999}, language = {en} } @article{BiselliSchmidtBuechsetal.1999, author = {Biselli, Manfred and Schmidt, S. and B{\"u}chs, J. and Born, C.}, title = {A new correlation for the wall-to-fluid mass transfer in liquid-sol fluidized beds / Schmidt, S. ; B{\"u}chs, J. ; Born, C. ; Biselli, M.}, series = {Chemical Engineering Science. 54 (1999), H. 6}, journal = {Chemical Engineering Science. 54 (1999), H. 6}, isbn = {0009-2509}, pages = {829 -- 839}, year = {1999}, language = {en} } @article{BiselliWandrey1988, author = {Biselli, Manfred and Wandrey, Christian}, title = {Reaction Mechanism of an Endoglucanase of Trichoderma Reesei QM 9414 using Cellodextrins as Substrate}, series = {Technology of biological processes, safety in biotechnology, applied genetic engineering : lectures held at the 5th DECHEMA Annual Meeting of Biotechnologists, May 12 - 13, 1987, [Frankfurt, Main] / [hrsg. von Dieter Behrens ...]}, journal = {Technology of biological processes, safety in biotechnology, applied genetic engineering : lectures held at the 5th DECHEMA Annual Meeting of Biotechnologists, May 12 - 13, 1987, [Frankfurt, Main] / [hrsg. von Dieter Behrens ...]}, publisher = {VCH}, address = {Weinheim}, pages = {425 -- 429}, year = {1988}, language = {en} } @article{BiselliSchmidWandrey1988, author = {Biselli, Manfred and Schmid, Georg and Wandrey, Christian}, title = {Preparation of cellodextrins and isolation of oligomeric side components and their characterization / Schmid, Georg ; Biselli, Manfred ; Wandrey, Christian}, series = {Analytical Biochemistry. 175 (1988)}, journal = {Analytical Biochemistry. 175 (1988)}, isbn = {0003-2697}, pages = {573 -- 583}, year = {1988}, language = {en} } @article{BiselliThoemmesGarskeetal.1992, author = {Biselli, Manfred and Th{\"o}mmes, J. and Garske, U. and Wandrey, Christian}, title = {Integrated detoxification: Reduction of ammonium concentration by dialysis with cation exchange membranes / Th{\"o}mmes, J. ; Garske, U. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750604212}, pages = {171 -- 175}, year = {1992}, language = {en} } @article{MangRoosenAnsorgeSchumacheretal.2007, author = {Mang, Thomas and Roosen, Christoph and Ansorge-Schumacher, Marion and Leitner, Walter}, title = {Gaining pH-control in water/carbon dioxide biphasic systems / Roosen, Christoph ; Ansorge-Schumacher, Marion ; Mang, Thomas ; Leitner, Walter ; Greiner, Lasse}, series = {Green Chemistry. 9 (2007)}, journal = {Green Chemistry. 9 (2007)}, isbn = {1463-9262}, pages = {455 -- 458}, year = {2007}, language = {en} } @article{MangKolzMartinsetal.2007, author = {Mang, Thomas and Kolz, J. and Martins, J. and Kremer, K.}, title = {Investigation of the Elastomer-Foam Production with Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Mang, Thomas ; Bl{\"u}mich, B.}, series = {Kautschuk Gummi Kunststoffe. 60 (2007), H. 4}, journal = {Kautschuk Gummi Kunststoffe. 60 (2007), H. 4}, isbn = {0948-3276}, pages = {179 -- 183}, year = {2007}, language = {en} } @article{KotterLiRiekert1990, author = {Kotter, Michael and Li, D. X. and Riekert, Lothar}, title = {Partial oxidation of o-xylene to phthalic anhydride in a structured fixed bed containing a sequence of catalysts}, series = {New developments in selective oxidation : proceedings of an international symposium ; Rimini, Italy, Sept. 18 - 22, 1989 / eds.: G. Centi ... - (Studies in surface science and catalysis ; 55)}, journal = {New developments in selective oxidation : proceedings of an international symposium ; Rimini, Italy, Sept. 18 - 22, 1989 / eds.: G. Centi ... - (Studies in surface science and catalysis ; 55)}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0-444-88694-X}, pages = {267 -- 274}, year = {1990}, language = {en} } @article{KotterHammonRiekert1988, author = {Kotter, Michael and Hammon, Ulrich and Riekert, Lothar}, title = {Formation of ethene and propene from methanol on zeolite ZSM-5 II. Preparation of finished catalysts and operation of a fixed-bed pilot plant / U. Hammon ; M. Kotter ; L. Riekert}, series = {Applied catalysis. 37 (1988)}, journal = {Applied catalysis. 37 (1988)}, isbn = {0166-9834}, pages = {155 -- 174}, year = {1988}, language = {en} } @article{KotterLintzRiekertetal.1989, author = {Kotter, Michael and Lintz, Hans-G{\"u}nther and Riekert, Lothar and Trimm, D. L.}, title = {Structure and selectivity changes in vanadia-titania catalysts used to promote the reduction of nitric oxide with ammonia}, series = {Applied catalysis. 52 (1989), H. 1}, journal = {Applied catalysis. 52 (1989), H. 1}, isbn = {0166-9834}, pages = {225 -- 235}, year = {1989}, language = {en} } @article{Mang2004, author = {Mang, Thomas}, title = {Thermosensitive Magnetic Nanoparticles for Bioanalytical and Therapeutical Applications}, series = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, journal = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, isbn = {0013-5585}, pages = {1006 -- 1007}, year = {2004}, language = {en} } @article{Mang2004, author = {Mang, Thomas}, title = {Polymer Magnetic Particles for Isolating Biomolecules, Especially Nucleic Acids}, series = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, journal = {Biomedizinische Technik = Biomedical Engineering. 49 (2004), H. 2}, isbn = {0013-5585}, pages = {1008 -- 1009}, year = {2004}, language = {en} } @article{MangKolzMartinsetal.2006, author = {Mang, Thomas and Kolz, J. and Martins, J. and Kremer, K.}, title = {Investigation of Elastomer Foams by Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Bl{\"u}mich, B. ; Mang, Thomas}, series = {Kurzfassungen : 3. bis 6. Juli 2006, N{\"u}rnberg = Summaries / Deutsche Kautschuk Tagung, 3.7.-6.7.2006}, journal = {Kurzfassungen : 3. bis 6. Juli 2006, N{\"u}rnberg = Summaries / Deutsche Kautschuk Tagung, 3.7.-6.7.2006}, publisher = {DKG, Dt. Kautschuk-Ges.}, address = {Frankfurt, Main}, pages = {33}, year = {2006}, language = {en} } @article{MangKricheldorf1983, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {Stereospecificity of peptide synthesis by means of phosphorus derivatives: a model of peptide synthesis in molecular evolution / Kricheldorf, Hans R. ; Mang, Thomas}, series = {International Journal of Biological Macromolecules. 5 (1983), H. 5}, journal = {International Journal of Biological Macromolecules. 5 (1983), H. 5}, isbn = {0141-8130}, pages = {258 -- 266}, year = {1983}, language = {en} } @article{MangKricheldorfJonte1984, author = {Mang, Thomas and Kricheldorf, Hans R. and Jont{\´e}, J. Michael}, title = {Polylactones. 1. Copolymerizations of glycolide and \&beta-caprolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jont{\´e}, J. Michael}, series = {Macromolecules. 17 (1984), H. 10}, journal = {Macromolecules. 17 (1984), H. 10}, isbn = {0024-9297}, pages = {2173 -- 2181}, year = {1984}, language = {en} } @article{MangKricheldorfJonte1985, author = {Mang, Thomas and Kricheldorf, Hans R. and Jont{\´e}, J. Michael}, title = {Polylactones, 2. Copolymerization of glycolide with \&\#946;-propiolactone, \&\#967;-butyrolactone or \&\#948;-valerolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jont{\´e}, J. Michael}, series = {Die makromolekulare Chemie. 186 (1985), H. 5}, journal = {Die makromolekulare Chemie. 186 (1985), H. 5}, isbn = {1022-1352}, pages = {955 -- 976}, year = {1985}, language = {en} } @article{MangKricheldorfAu1985, author = {Mang, Thomas and Kricheldorf, Hans R. and Au, M.}, title = {Models of molecular evolution. 2. Stereospecificity of dipeptide syntheses by means of cyanamides and carbodiimides / Kricheldorf, Hans R. ; Au, M. ; Mang, Thomas}, series = {International journal of peptide and protein research. 26 (1985), H. 2}, journal = {International journal of peptide and protein research. 26 (1985), H. 2}, isbn = {0300-9769}, pages = {149 -- 157}, year = {1985}, language = {en} } @article{MangHaulena1992, author = {Mang, Thomas and Haulena, Friedhelm}, title = {Monodisperse latex particles with functional groups}, series = {Modification of polymers : 4th Dresden Polymer Discussion ; September 22 to 25, 1992 at Gaussig / Dresden University of Technology, Department of Macromolecular Chemistry and Textile Chemistry; Institute of Polymer Technology Dresden. [Organizers: Claudia Kummerl{\"o}we ...]}, journal = {Modification of polymers : 4th Dresden Polymer Discussion ; September 22 to 25, 1992 at Gaussig / Dresden University of Technology, Department of Macromolecular Chemistry and Textile Chemistry; Institute of Polymer Technology Dresden. [Organizers: Claudia Kummerl{\"o}we ...]}, publisher = {Univ.}, address = {Dresden}, pages = {68 -- 88}, year = {1992}, language = {en} } @article{MangKricheldorf1981, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {13-C-NMR Sequence Analysis 20. Stereospecificity of the polymerization of D,L-Leu-NCA and D,L-Val-NCA / Kricheldorf, Hans R. ; Mang, Thomas}, series = {Die makromolekulare Chemie. 182 (1981), H. 11}, journal = {Die makromolekulare Chemie. 182 (1981), H. 11}, isbn = {1022-1352}, pages = {3077 -- 3098}, year = {1981}, language = {en} } @article{MangKricheldorf1982, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {13-C-NMR sequence analysis. 21. Stereoselectivity of oligopeptide syntheses / Kricheldorf, Hans R. ; Mang, Thomas}, series = {Die makromolekulare Chemie. 183 (1982), H. 9}, journal = {Die makromolekulare Chemie. 183 (1982), H. 9}, isbn = {1022-1352}, pages = {2093 -- 2111}, year = {1982}, language = {en} } @article{MangKricheldorf1982, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {13-C-NMR sequence analysis. 22. Stereoselectivity of the polymerization of D,L-leucine and D,L-valine N-thiocarboxy anhydrides / Kricheldorf, Hans R. ; Mang, Thomas}, series = {Die makromolekulare Chemie. 183 (1982), H. 9}, journal = {Die makromolekulare Chemie. 183 (1982), H. 9}, isbn = {1022-1352}, pages = {2113 -- 2129}, year = {1982}, language = {en} } @article{KotterRiekertWeyland1985, author = {Kotter, Michael and Riekert, Lothar and Weyland, Friedrich}, title = {The performance of base metal oxides on ceramic carriers as catalysts for air pollution control}, series = {International chemical engineering. 25 (1985), H. 3}, journal = {International chemical engineering. 25 (1985), H. 3}, isbn = {0020-6318}, pages = {418 -- 427}, year = {1985}, language = {en} } @article{KotterGregerIhme1984, author = {Kotter, Michael and Greger, M. and Ihme, B.}, title = {Nonequilibrium phase transitions with hysteresis in solid catalysts : the system copper-oxygen-propene / M. Greger ; B. Ihme ; M. Kotter ...}, series = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 88 (1984)}, journal = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 88 (1984)}, isbn = {0005-9021}, pages = {427 -- 433}, year = {1984}, language = {en} } @article{KotterLintzTurek1992, author = {Kotter, Michael and Lintz, Hans-G{\"u}nther and Turek, Thomas}, title = {Selective catalytic reduction of nitrogen oxide by use of the Ljungstroem air heater as reactor : a case study}, series = {Chemical engineering science . 47 (1992), H. 9-11}, journal = {Chemical engineering science . 47 (1992), H. 9-11}, isbn = {0009-2509}, pages = {2763 -- 2768}, year = {1992}, language = {en} } @article{MangHaulena1998, author = {Mang, Thomas and Haulena, Friedhelm}, title = {Recycling of Polymeric Composites}, series = {Invited and contributing lectures presented at the 38th Microsymposium on Recycling of Polymers : held in Prague, Czech Republic, July 14 - 17, 1997 / International Union of Pure and Applied Chemistry, Macromolecular Division. Symposium ed. J. Kahovec.}, journal = {Invited and contributing lectures presented at the 38th Microsymposium on Recycling of Polymers : held in Prague, Czech Republic, July 14 - 17, 1997 / International Union of Pure and Applied Chemistry, Macromolecular Division. Symposium ed. J. Kahovec.}, publisher = {Wiley-VCH}, address = {Weinheim}, pages = {147 -- 156}, year = {1998}, language = {en} } @article{BaumannGradinger1991, author = {Baumann, Marcus and Gradinger, R.}, title = {Distribution of Phytoplankton Communities in Relation to the Large Scale Hydrographical Regime in the Fram Strait 1984 / Gradinger, R. ; Baumann, M.E.M.}, series = {Marine Biology. 111 (1991), H. 2}, journal = {Marine Biology. 111 (1991), H. 2}, isbn = {0025-3162}, pages = {311 -- 321}, year = {1991}, language = {en} } @article{BaumannHircheKattneretal.1991, author = {Baumann, Marcus and Hirche, H.-J. and Kattner, G. and Gradinger, R.}, title = {Plankton distribution and the impact of copepod grazing on primary production in Fram Strait, Greenland Sea / Hirche, H.-J. ; Baumann, M.E.M. ; Kattner, G.; Gradinger, R.}, series = {Journal of Marine Systems. 2 (1991), H. 3-4}, journal = {Journal of Marine Systems. 2 (1991), H. 3-4}, isbn = {0924-7963}, pages = {477 -- 494}, year = {1991}, language = {en} } @article{BaumannThomasGleitz1992, author = {Baumann, Marcus and Thomas, D. and Gleitz, M.}, title = {Efficiency of carbon assimilation and photoacclimation in a small unicellular Chaetoceros species from the Weddel Sea (Antarctica): Influence of temperature and irridiance / Thomas, D. ; Baumann, M.E.M. ; Gleitz, M.}, series = {Journal of Experimental Marine Biology and Ecology. 157 (1992), H. 2}, journal = {Journal of Experimental Marine Biology and Ecology. 157 (1992), H. 2}, isbn = {0022-0981}, pages = {195 -- 209}, year = {1992}, language = {en} } @article{BaumannBrandiniStaubes1994, author = {Baumann, Marcus and Brandini, Frederico and Staubes, Regina}, title = {The influence of light and temperature on carbonspecific DMS release by cultures of Phaeocystis antarctica and three antarctic diatoms / Baumann, Marcus E.M. ; Brandini, Frederico ; Staubes, Regina}, series = {Marine Chemistry. 45 (1994), H. 1-2}, journal = {Marine Chemistry. 45 (1994), H. 1-2}, isbn = {0304-4203}, pages = {129 -- 136}, year = {1994}, language = {en} } @article{BaumannLancelotBrandinietal.1994, author = {Baumann, Marcus and Lancelot, C. and Brandini, F. and Sakshaug, E.}, title = {The taxonomic identity of the cosmopolitan prymnesiophyte Phaeocystis, a morphological and ecophysiological approach / Baumann, M.E.M. ; Lancelot, C. ; Brandini, F. ; Sakshaug, E. ; John M.}, series = {Journal of Marine Systems. 5 (1994), H. 1}, journal = {Journal of Marine Systems. 5 (1994), H. 1}, isbn = {0924-7963}, pages = {5 -- 22}, year = {1994}, language = {en} } @article{BaumannRousseauVaulotetal.1994, author = {Baumann, Marcus and Rousseau, V. and Vaulot, D. and Casotti, R.}, title = {The life cycle of Phaeocystis (Prymnesiophycae): evidence and hypotheses / Rousseau, V. ; Vaulot, D. ; Casotti, R. ; Cariou, V. ; Lenz, J. ; Gunkel, J. ; Baumann, M.}, series = {Journal of Marine Systems. 5 (1994), H. 1}, journal = {Journal of Marine Systems. 5 (1994), H. 1}, isbn = {0924-7963}, pages = {23 -- 39}, year = {1994}, language = {en} } @article{BaumannLaraHubbertenetal.1997, author = {Baumann, Marcus and Lara, R.J. and Hubberten, D.N. and Thomas, D.N.}, title = {Dissolved organic matter studies in enclosed systems: Application of hydophobic fractionation for the assessment of organic nitrogen dynamics / Lara, R.J. ; Hubberten, U. ; Thomas, D.N. ; Baumann, M.E.M. ; Kattner, G.}, series = {Journal of Marine Systems. 13 (1997), H. 1-4}, journal = {Journal of Marine Systems. 13 (1997), H. 1-4}, isbn = {0924-7963}, pages = {155 -- 161}, year = {1997}, language = {en} } @article{BaumannGoeyensSemenehetal.1998, author = {Baumann, Marcus and Goeyens, L. and Semeneh, M. and Elskens, M.}, title = {Phytoplankton nutrient utilisation and nutrient signature in the Southern Ocean / Goeyens, L. ; Semeneh, M. ; Baumann, M.E.M. ; Elskens, M. ; Shopova, D. ; Dehairs, F.}, series = {Journal of Marine Systems. 17 (1998), H. 1-4}, journal = {Journal of Marine Systems. 17 (1998), H. 1-4}, isbn = {0924-7963}, pages = {143 -- 157}, year = {1998}, language = {en} } @article{BaumannSemenehDehairsetal.1998, author = {Baumann, Marcus and Semeneh, M. and Dehairs, F. and Elskens, M.}, title = {Nitrogen uptake regime and phytoplankton community structure in the Southern Ocean / Semeneh, M. ; Dehairs, F. ; Elskens, M. ; Baumann, M.E.M. ; Kopczynska, E.E. ; Lancelot, C. ; Goeyens, L.}, series = {Journal of Marine Systems. 17 (1998), H. 1-4}, journal = {Journal of Marine Systems. 17 (1998), H. 1-4}, isbn = {0924-7963}, pages = {159 -- 177}, year = {1998}, language = {en} } @article{BaumannJahnke1983, author = {Baumann, Marcus and Jahnke, J.}, title = {Chemical and Physical Effects on the Shape and Growth of the Diatom Biddulphia sinensis GREVILLE in Batch Cultures: A Contribution to Bioindication in Plankton Ecology / Jahnke, J. ; Baumann, M.}, series = {Hydrobiological Bulletin . 17 (1983), H. 1}, journal = {Hydrobiological Bulletin . 17 (1983), H. 1}, isbn = {0165-1404}, pages = {5 -- 20}, year = {1983}, language = {en} } @article{WincklerKruegerSchnitzleretal.2014, author = {Winckler, Silvia and Krueger, Rolf and Schnitzler, Thomas and Zang, Werner and Fischer, Rainer and Biselli, Manfred}, title = {A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach}, series = {Bioprocess and biosystems engineering}, volume = {37}, journal = {Bioprocess and biosystems engineering}, number = {5}, publisher = {Springer}, address = {Berlin, Heidelberg}, issn = {1615-7591 (Print) 1615-7605 (Online)}, doi = {10.1007/s00449-013-1062-8}, pages = {901 -- 912}, year = {2014}, abstract = {Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.}, language = {en} } @article{Schnitzler2009, author = {Schnitzler, Thomas}, title = {Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)}, series = {Application notes / Sartorius stedim biotech}, journal = {Application notes / Sartorius stedim biotech}, pages = {1 -- 4}, year = {2009}, language = {en} } @article{HemmerlingMerschenzQuackWunderlich1988, author = {Hemmerling, H.-J. and Merschenz-Quack, Angelika and Wunderlich, H.}, title = {Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988}, series = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, volume = {185}, journal = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, number = {H. 1-4}, issn = {2196-7105 (E-Books); 2194-4946 (Print)}, pages = {256}, year = {1988}, language = {en} } @article{HeinzeMangPeteretal.2014, author = {Heinze, Daniel and Mang, Thomas and Peter, Karin and M{\"o}ller, Martin and Weichold, Oliver}, title = {Synthesis of low molecular weight poly(vinyl acetate) and its application as plasticizer}, series = {Journal of applied polymer science}, volume = {131}, journal = {Journal of applied polymer science}, number = {9}, publisher = {Wiley}, address = {New York}, issn = {1097-4628 (E-Journal); 0021-8995 (Print)}, doi = {10.1002/app.40226}, pages = {Article No. 40226}, year = {2014}, abstract = {Poly(vinyl acetate), PVAc, with a degree of polymerization Xn = 10 was prepared by chain-transfer radical polymerization using carbon tetrachloride and used as oligomeric plasticizer for commercial PVAc. However, the chlorinated chain ends cause a low thermal stability requiring mild Cl/H substitution. The product exhibits high thermal stability and excellent melt-compounding properties. Blends of oligomeric and commercial PVAc show single glass transition temperatures which decrease with higher oligomer content and exhibit small negative deviations from Fox' linear additivity rule. This indicates plasticization and miscibility being mainly due to entropic effects. Injection-moulded thick specimens show ductile behaviour at oligomer contents >10 wt \%, while sheets with a thickness of 0.2-0.5 mm appear flexible already at 7.5 wt \%. The oxygen permeability coefficients are an order of magnitude lower than those of low-density polyethylene. Due to the sum of their properties, the plasticized sheets present a promising alternative in the preparation of barrier materials.}, language = {en} } @article{DuttaHartkopfFroederWitteetal.2013, author = {Dutta, Suryendu and Hartkopf-Fr{\"o}der, Christoph and Witte, Karin and Brocke, Rainer and Mann, Ulrich}, title = {Molecular characterization of fossil palynomorphs by transmission micro-FTIR spectroscopy: implications for hydrocarbon source evaluation}, series = {International journal of coal geology}, volume = {Vol. 115}, journal = {International journal of coal geology}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1872-7840 (E-Journal); 0166-5162 (Print)}, pages = {13 -- 23}, year = {2013}, language = {en} } @article{WangDruckenmuellerElbersetal.2014, author = {Wang, Ren-Qi and Druckenm{\"u}ller, Katharina and Elbers, Gereon and Guenther, Klaus and Crou{\´e}, Jean-Philippe}, title = {Analysis of aquatic-phase natural organic matter by optimized LDI-MS method}, series = {Journal of mass spectrometry}, volume = {49}, journal = {Journal of mass spectrometry}, number = {2}, publisher = {Wiley}, address = {Bognor Regis}, issn = {1096-9888}, doi = {10.1002/jms.3321}, pages = {154 -- 160}, year = {2014}, abstract = {The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200-1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200-15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley \& Sons, Ltd.}, language = {en} } @article{HeineHerrmannSelmeretal.2014, author = {Heine, A. and Herrmann, G. and Selmer, Thorsten and Terwesten, F. and Buckel, W. and Reuter, K.}, title = {High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily}, series = {Proteins}, volume = {82}, journal = {Proteins}, number = {9}, publisher = {Wiley-Liss}, address = {New York}, issn = {1097-0134 (E-Journal); 0887-3585 (Print)}, doi = {10.1002/prot.24557}, pages = {2041 -- 2053}, year = {2014}, abstract = {Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 {\AA} as well as in complex with CoA at a resolution of 1.59 {\AA}. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called "hot dog fold" which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all "hot dog fold" proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041-2053. © 2014 Wiley Periodicals, Inc.}, language = {en} } @article{TakenagaBiselliSchnitzleretal.2014, author = {Takenaga, Shoko and Biselli, Manfred and Schnitzler, Thomas and {\"O}hlschl{\"a}ger, Peter and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing}, series = {Physica status solidi A : Applications and materials science}, volume = {211}, journal = {Physica status solidi A : Applications and materials science}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)}, doi = {10.1002/pssa.201330464}, pages = {1410 -- 1415}, year = {2014}, abstract = {The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.}, language = {en} } @article{GuoMiyamotoWagneretal.2014, author = {Guo, Yuanyuan and Miyamoto, Ko-ichiro and Wagner, Torsten and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Theoretical study and simulation of light-addressable potentiometric sensors}, series = {Physica status solidi (A) : applications and materials}, volume = {211}, journal = {Physica status solidi (A) : applications and materials}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0031-8965}, doi = {10.1002/pssa.201330354}, pages = {1467 -- 1472}, year = {2014}, abstract = {The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance.}, language = {en} } @article{SmithBaumannWilsonetal.1987, author = {Smith, Walker O. and Baumann, Marcus and Wilson, David L. and Aletsee, Ludwig}, title = {Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984}, series = {Journal of geophysical research}, volume = {Vol. 92}, journal = {Journal of geophysical research}, number = {Iss. C7}, issn = {2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print)}, pages = {6777 -- 6786}, year = {1987}, language = {en} } @article{JahnkeBaumann1987, author = {Jahnke, J. and Baumann, Marcus}, title = {Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel}, series = {Hydrobiological bulletin}, volume = {Vol. 21}, journal = {Hydrobiological bulletin}, number = {Iss. 2}, issn = {0165-1404 (Print); 1573-5125 (E-Journal)}, pages = {141 -- 147}, year = {1987}, language = {en} } @article{AletseeBaumann1991, author = {Aletsee, Ludwig and Baumann, Marcus}, title = {A laboratory incubator equipped with facilities to automatically simulate natural irradiance}, series = {Boletim do Instituto Oceanogr{\´a}fico / Universidade de S{\~a}o Paulo}, volume = {Vol. 39}, journal = {Boletim do Instituto Oceanogr{\´a}fico / Universidade de S{\~a}o Paulo}, number = {No. 2}, issn = {1982-436X; 0080-6331}, pages = {155 -- 159}, year = {1991}, language = {en} } @article{TuegBaumann1994, author = {T{\"u}g, Helmut and Baumann, Marcus}, title = {Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements}, series = {Geophysical research letters}, volume = {Vol. 21}, journal = {Geophysical research letters}, number = {Iss. 8}, issn = {1944-8007 (E-Journal); 0094-8276 (Print)}, pages = {689 -- 692}, year = {1994}, language = {en} } @article{MedlinLangeBaumann1994, author = {Medlin, L. K. and Lange, M. and Baumann, Marcus}, title = {Genetic differentiation among three colony-forming species of Phaeocystis : further evidence for the phylogeny of the Prymnesiophyta}, series = {Phycologia}, volume = {Vol. 33}, journal = {Phycologia}, number = {Iss. 3}, issn = {0031-8884}, pages = {199 -- 212}, year = {1994}, language = {en} } @article{TuegBaumann1995, author = {T{\"u}g, Helmut and Baumann, Marcus}, title = {Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper "Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements"}, series = {Geophysical research letters}, volume = {Vol. 22}, journal = {Geophysical research letters}, number = {Iss. 9}, issn = {1944-8007 (E-Journal); 0094-8276 (Print)}, pages = {1159 -- 1160}, year = {1995}, language = {en} } @article{MartinezJakobTuetal.2013, author = {Martinez, Ronny and Jakob, Felix and Tu, Ran and Siegert, Petra and Maurer, Karl-Heinz and Schwaneberg, Ulrich}, title = {Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution}, series = {Biotechnology and bioengineering}, volume = {Vol. 110}, journal = {Biotechnology and bioengineering}, number = {Iss. 3}, publisher = {Wiley}, address = {Weinheim}, issn = {1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print)}, pages = {711 -- 720}, year = {2013}, language = {en} } @article{JakobMartinezMandaweetal.2013, author = {Jakob, Felix and Martinez, Ronny and Mandawe, John and Hellmuth, Hendrik and Siegert, Petra and Maurer, Karl-Heinz and Schwaneberg, Ulrich}, title = {Surface charge engineering of a Bacillus gibsonii subtilisin protease}, series = {Applied microbiology and biotechnology}, volume = {Vol. 97}, journal = {Applied microbiology and biotechnology}, number = {Iss. 15}, publisher = {Springer}, address = {Berlin}, issn = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)}, pages = {6793 -- 6802}, year = {2013}, language = {en} } @article{NiehausGaborWielandetal.2011, author = {Niehaus, F. and Gabor, E. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Eck, J.}, title = {Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases}, series = {Microbial biotechnology}, volume = {Vol. 4}, journal = {Microbial biotechnology}, number = {Iss. 6}, publisher = {Springer}, address = {Berlin}, issn = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)}, pages = {767 -- 776}, year = {2011}, language = {en} } @article{RibitschHeumannKarletal.2012, author = {Ribitsch, D. and Heumann, S. and Karl, W. and Gerlach, J. and Leber, R. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Siegert, Petra and Lange, J. and Maurer, Karl-Heinz and Berg, G. and Guebitz, G. M. and Schwab, H.}, title = {Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli}, series = {Journal of biotechnology}, volume = {157}, journal = {Journal of biotechnology}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2011.09.025}, pages = {140 -- 147}, year = {2012}, abstract = {A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @article{SiegertMcLeishBaumannetal.2005, author = {Siegert, Petra and McLeish, Michael J. and Baumann, Martin and Iding, Hans and Kneen, Malea M. and Kenyon, George L. and Pohl, Martina}, title = {Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida}, series = {Protein engineering, design, and selection : peds}, volume = {Vol. 18}, journal = {Protein engineering, design, and selection : peds}, number = {Iss. 7}, issn = {1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print)}, pages = {345 -- 357}, year = {2005}, language = {en} } @article{DuenkelmannKolterJungNitscheetal.2002, author = {D{\"u}nkelmann, Pascal and Kolter-Jung, Doris and Nitsche, Adam and Demir, Ayhan S. and Siegert, Petra and Lingen, Bettina and Baumann, Martin and Pohl, Martina and M{\"u}ller, Michael}, title = {Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation}, series = {Journal of the American Chemical Society}, volume = {Vol. 124}, journal = {Journal of the American Chemical Society}, issn = {1520-5126 (E-Journal); 0002-7863 (Print)}, pages = {12084 -- 12085}, year = {2002}, language = {en} } @article{DuennwaldDemirSiegertetal.2001, author = {D{\"u}nnwald, Thomas and Demir, Ayhan S. and Siegert, Petra and Pohl, Martina and M{\"u}ller, Michael}, title = {ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation}, series = {Cheminform}, volume = {Vol. 32}, journal = {Cheminform}, number = {Iss. 4}, issn = {1522-2667 (E-Journal); 0931-7597 (Print)}, pages = {Publ. online}, year = {2001}, language = {en} } @article{DuennwaldDemirSiegertetal.2000, author = {D{\"u}nnwald, Thomas and Demir, Ayhan S. and Siegert, Petra and Pohl, Martina and M{\"u}ller, Michael}, title = {Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C-C Bond Formation}, series = {European journal of organic chemistry}, volume = {Vol. 2000}, journal = {European journal of organic chemistry}, number = {Iss. 11}, issn = {0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print)}, pages = {2161 -- 2170}, year = {2000}, language = {en} } @article{IdingDuennwaldGreineretal.2000, author = {Iding, Hans and D{\"u}nnwald, Thomas and Greiner, Lasse and Liese, Andreas and M{\"u}ller, Michael and Siegert, Petra and Gr{\"o}tzinger, Joachim and Demir, Ayhan S. and Pohl, Martina}, title = {Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones}, series = {Chemistry - a European journal}, volume = {Vol. 6}, journal = {Chemistry - a European journal}, number = {Iss. 8}, issn = {1521-3765 (E-Journal); 0947-6539 (Print)}, pages = {1483 -- 1495}, year = {2000}, language = {en} } @article{WhiteheadOehlschlaegerAlmajhdietal.2014, author = {Whitehead, Mark and {\"O}hlschl{\"a}ger, Peter and Almajhdi, Fahad N. and Alloza, Leonor and Marz{\´a}bal, Pablo and Meyers, Ann E. and Hitzeroth, Inga I. and Rybicki, Edward P.}, title = {Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice}, series = {BMC cancer}, journal = {BMC cancer}, number = {14:367}, publisher = {BioMed Central}, address = {London}, issn = {1471-2407}, doi = {10.1186/1471-2407-14-367}, pages = {1 -- 15}, year = {2014}, language = {en} } @article{IdingSiegertMeschetal.1998, author = {Iding, Hans and Siegert, Petra and Mesch, K. and Pohl, Martina}, title = {Application of α-keto acid decarboxylases in biotransformations}, series = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology}, volume = {Vol. 1385}, journal = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology}, number = {Iss. 2}, issn = {1879-2588 (E-Journal); 0167-4838 (Print)}, pages = {307 -- 322}, year = {1998}, language = {en} } @article{PohlSiegertMeschetal.1998, author = {Pohl, Martina and Siegert, Petra and Mesch, K. and Bruhn, H. and Gr{\"o}tzinger, Joachim}, title = {Active site mutants of pyruvate decarboxylase from Zymomonas mobilis : a site-directed mutagenesis study of L112, I472, I476, E473 and N482}, series = {European journal of biochemistry}, volume = {Vol. 257}, journal = {European journal of biochemistry}, number = {Iss. 3}, issn = {1432-1033 (E-Journal); 1742-4658 (E-Journal); 0014-2956 (Print); 1742-464X (Print)}, pages = {538 -- 546}, year = {1998}, language = {en} } @article{RaueWambachGloeggleretal.2014, author = {Raue, Markus and Wambach, M. and Gl{\"o}ggler, S. and Grefen, Dana and Kaufmann, R. and Abetz, C. and Georgopanos, P. and Handge, U. A. and Mang, Thomas and Bl{\"u}mich, B. and Abetz, V.}, title = {Investigation of historical hard rubber ornaments of Charles Goodyear}, series = {Macromolecular chemistry and physics}, volume = {Vol. 215}, journal = {Macromolecular chemistry and physics}, number = {No. 3}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1022-1352}, pages = {245 -- 254}, year = {2014}, language = {en} } @article{PilasIkenSelmeretal.2015, author = {Pilas, Johanna and Iken, Heiko and Selmer, Thorsten and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Development of a multi-parameter sensor chip for the simultaneous detection of organic compounds in biogas processes}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431894}, pages = {1306 -- 1312}, year = {2015}, abstract = {An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.}, language = {en} } @article{BreuerRaueKirschbaumetal.2015, author = {Breuer, Lars and Raue, Markus and Kirschbaum, M. and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, R. and Wagner, Torsten}, title = {Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431944}, pages = {1368 -- 1374}, year = {2015}, abstract = {Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.}, language = {en} } @article{SchiffelsSelmer2015, author = {Schiffels, Johannes and Selmer, Thorsten}, title = {A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro}, series = {Biotechnology and Bioengineering}, volume = {112}, journal = {Biotechnology and Bioengineering}, number = {11}, publisher = {Wiley}, address = {Weinheim}, issn = {1097-0290}, doi = {10.1002/bit.25658}, pages = {2360 -- 2372}, year = {2015}, language = {en} } @article{PilasMarianoKeusgenetal.2015, author = {Pilas, Johanna and Mariano, K. and Keusgen, M. and Selmer, Thorsten and Sch{\"o}ning, Michael Josef}, title = {Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes}, series = {Procedia Engineering}, volume = {120}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2015.08.702}, pages = {532 -- 535}, year = {2015}, language = {en} } @article{CehreliAkpinarTemizArtmannetal.2015, author = {Cehreli, Ruksan and Akpinar, Hale and Temiz Artmann, Ayseg{\"u}l and Sagol, Ozgul}, title = {Effects of Glutamine and Omega-3 Fatty Acids on Erythrocyte Deformability and Oxidative Damage in Rat Model of Enterocolitis}, series = {Gastroenterology Research}, volume = {8}, journal = {Gastroenterology Research}, number = {5}, issn = {1918-2813}, doi = {10.14740/gr683w}, pages = {265 -- 273}, year = {2015}, language = {en} } @article{NachtrodtTietschMostaccietal.2014, author = {Nachtrodt, Frederik and Tietsch, Wolfgang and Mostacci, Domiziano and Scherer, Ulrich W.}, title = {Set-up and first operation of a plasma oven for treatment of low level radioactive wastes}, series = {Nuclear technology and radiation protection}, volume = {29}, journal = {Nuclear technology and radiation protection}, number = {Suppl.}, publisher = {VINČA Institute of Nuclear Sciences}, address = {Belgrad}, issn = {1451-3994}, doi = {10.2298/NTRP140SS47N}, pages = {47 -- 51}, year = {2014}, language = {en} } @article{RatkeMilowLisinskietal.2014, author = {Ratke, Lorenz and Milow, Barbara and Lisinski, Susanne and Hoepfner, Sandra}, title = {On an effect of fine ceramic particles on the structure of aerogels}, series = {Microgravity science and technology}, volume = {26}, journal = {Microgravity science and technology}, publisher = {Springer Nature}, address = {Heidelberg}, issn = {0938-0108 ; 1875-0494}, doi = {10.1007/s12217-014-9380-2}, pages = {103 -- 110}, year = {2014}, language = {en} } @article{SeiblerKleinriddersKueterLuksetal.2007, author = {Seibler, Jost and Kleinridders, Andre and K{\"u}ter-Luks, Birgit and Niehaves, Sandra and Br{\"u}ning, Jens C. and Schwenk, Frieder}, title = {Reversible gene knockdown in mice using a tight, inducible shRNA expression system}, series = {Nucleic Acids Research}, volume = {35}, journal = {Nucleic Acids Research}, number = {7}, issn = {1362-4962}, doi = {10.1093/nar/gkm122}, pages = {e54}, year = {2007}, language = {en} } @article{PlumMaHampeletal.2006, author = {Plum, Leona and Ma, Xiaosong and Hampel, Brigitte and Balthasar, Nina and Coppari, Roberto and M{\"u}nzberg, Heike and Shanabrough, Marya and Burdakov, Denis and Rother, Eva and Janoschek, Ruth and Alber, Jens and Belgardt, Bengt F. and Koch, Linda and Seibler, Jost and Schenk, Frieder and Fekete, Csaba and Suzuki, Akira and Mak, Tak W. and Krone, Wilhelm and Horvath, Tamas L. and Ashcroft, Frances M. and Br{\"u}ning, Jens C.}, title = {Enhanced PIP3 signaling in POMC neurons causes KATP channel activation and leads to diet-sensitive obesity}, series = {The Journal of Clinical Investigation (JCI)}, volume = {116}, journal = {The Journal of Clinical Investigation (JCI)}, number = {7}, issn = {1558-8238}, doi = {10.1172/JCI27123}, pages = {1886 -- 1901}, year = {2006}, language = {en} } @article{SeiblerKueterLuksKernetal.2005, author = {Seibler, Jost and K{\"u}ter-Luks, Birgit and Kern, Heidrun and Streu, Sandra and Plum, Leona and Maurer, Jan and K{\"u}hn, Ralf and Br{\"u}ning, Jens C. and Schwenk, Frieder}, title = {Single copy shRNA configuration for ubiquitous gene knockdown in mice}, series = {Nucleic Acids Research}, volume = {33}, journal = {Nucleic Acids Research}, number = {7}, issn = {1362-4962}, doi = {10.1093/nar/gni065}, pages = {e67}, year = {2005}, language = {en} } @article{SeiblerZevnikKueterLuksetal.2003, author = {Seibler, Jost and Zevnik, Branko and K{\"u}ter-Luks, Birgit and Andreas, Susanne and Kern, Heidrun and Hennek, Thomas and Rode, Anja and Heimann, Cornelia and Faust, Nicole and Kauselmann, Gunther and Schoor, Michael and Jaenisch, Rudolf and Rajewsky, Klaus and K{\"u}hn, Ralf and Schwenk, Frieder}, title = {Rapid generation of inducible mouse mutants}, series = {Nucleic Acids Research}, volume = {33}, journal = {Nucleic Acids Research}, number = {4}, issn = {1362-4962}, doi = {10.1093/nar/gng012}, pages = {e12}, year = {2003}, language = {en} } @article{GoetzeBaerWinkelmannetal.2005, author = {Goetze, Sandra and Baer, Alexandra and Winkelmann, Silke and Nehlsen, Kristina and Seibler, Jost and Maass, Karin and Bode, J{\"u}rgen}, title = {Performance of genomic bordering elements at predefined genomic loci}, series = {Molecular and Cellular Biology}, volume = {25}, journal = {Molecular and Cellular Biology}, number = {6}, issn = {1098-5549}, doi = {10.1128/MCB.25.6.2260-2272.2005}, pages = {2260 -- 2272}, year = {2005}, language = {en} } @article{TakenagaSchneiderErbayetal.2015, author = {Takenaga, Shoko and Schneider, Benno and Erbay, E. and Biselli, Manfred and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef and Wagner, Torsten}, title = {Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201532053}, pages = {1347 -- 1352}, year = {2015}, abstract = {A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.}, language = {en} } @article{InagakiSleddensLinkelsWassenaaretal.2011, author = {Inagaki, Akiko and Sleddens-Linkels, Esther and Wassenaar, Evelyne and Ooms, Marja and Cappellen, Wiggert A. van and Hoeijmakers, Jan H. J. and Seibler, Jost and Vogt, Thomas F. and Shin, Myung K. and Grootegoed, J. Anton and Baarends, Willy M.}, title = {Meiotic functions of RAD18}, series = {Journal of Cell Science}, volume = {124}, journal = {Journal of Cell Science}, number = {16}, publisher = {Company of Biologists Limited}, address = {Cambridge}, issn = {1477-9137}, doi = {10.1242/jcs.081968}, pages = {2837 -- 2850}, year = {2011}, language = {en} } @article{RaabKappelKraemeretal.2011, author = {Raab, Monika and Kappel, Sven and Kr{\"a}mer, Andrea and Sanhaji, Mourad and Matthess, Yves and Kurunci-Csacsko, Elisabeth and Calzada-Wack, Julia and Rathkolb, Birgit and Rosman, Jan and Adler, Thure and Busch, Dirk H. and Esposito, Irene and Fuchs, Helmut and Gailus-Durner, Val{\´e}rie and Klingenspor, Martin and Wolf, Eckhard and S{\"a}nger, Nicole and Prinz, Florian and Hrabe de Angelis, Martin and Seibler, Jost and Yuan, Juping and Bergmann, Martin and Knecht, Rainald and Kreft, Bertolt and Strebhardt, Klaus}, title = {Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells}, series = {Nature Communications}, volume = {2}, journal = {Nature Communications}, number = {395}, publisher = {Nature}, address = {London}, issn = {2041-1723}, doi = {10.1038/ncomms1395}, pages = {1 -- 11}, year = {2011}, language = {en} } @article{HasegawaKapelyukhTaharaetal.2011, author = {Hasegawa, Maki and Kapelyukh, Yury and Tahara, Harunobu and Seibler, Jost and Rode, Anja and Krueger, Sylvia and Lee, Dongtao N. and Wolf, C. Roland and Scheer, Nico}, title = {Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line}, series = {Molecular Pharmacology}, volume = {80}, journal = {Molecular Pharmacology}, number = {33}, publisher = {ASPET}, address = {Bethesda, Md.}, issn = {1521-0111}, doi = {10.1124/mol.111.071845}, pages = {518 -- 528}, year = {2011}, language = {en} } @article{JordanKruegerWillmesetal.2011, author = {Jordan, Sabine D. and Kr{\"u}ger, Markus and Willmes, Diana M. and Redemann, Nora and Wunderlich, F. Thomas and Br{\"o}nneke, Hella S. and Merkwirth, Carsten and Kashkar, Hamid and Olkkonen, Vesa M. and B{\"o}ttger, Thomas and Braun, Thomas and Seibler, Jost and Br{\"u}ning, Jens C.}, title = {Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism}, series = {Nature Cell Biology}, volume = {13}, journal = {Nature Cell Biology}, number = {4}, publisher = {Nature}, address = {New York}, issn = {1465-7392}, doi = {10.1038/ncb2211}, pages = {434 -- 446}, year = {2011}, abstract = {The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143-145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143-ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.}, language = {en} }