@article{MangRoosenAnsorgeetal.2006, author = {Mang, Thomas and Roosen, C. and Ansorge, M. and Leitner, W.}, title = {Gaining pH-control in water/carbon dioxide biphasic systems / Abstract No. 1038 / Roosen, Ch. ; Ansorge, M. ; Mang, Thomas ; Leitner, W. ; Greiner, L.}, series = {Green solvents for processes : Lake Constance, Friedrichshafen, Germany, 8 - 11 October 2006 ; book of abstracts / DECHEMA e.V.}, journal = {Green solvents for processes : Lake Constance, Friedrichshafen, Germany, 8 - 11 October 2006 ; book of abstracts / DECHEMA e.V.}, publisher = {DECHEMA}, address = {Frankfurt am Main}, pages = {145 S.}, year = {2006}, language = {en} } @article{MangKricheldorf1983, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {Stereospecificity of peptide synthesis by means of phosphorus derivatives: a model of peptide synthesis in molecular evolution / Kricheldorf, Hans R. ; Mang, Thomas}, series = {International Journal of Biological Macromolecules. 5 (1983), H. 5}, journal = {International Journal of Biological Macromolecules. 5 (1983), H. 5}, isbn = {0141-8130}, pages = {258 -- 266}, year = {1983}, language = {en} } @article{MangKricheldorfJonte1984, author = {Mang, Thomas and Kricheldorf, Hans R. and Jont{\´e}, J. Michael}, title = {Polylactones. 1. Copolymerizations of glycolide and \&beta-caprolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jont{\´e}, J. Michael}, series = {Macromolecules. 17 (1984), H. 10}, journal = {Macromolecules. 17 (1984), H. 10}, isbn = {0024-9297}, pages = {2173 -- 2181}, year = {1984}, language = {en} } @article{MangKricheldorfJonte1985, author = {Mang, Thomas and Kricheldorf, Hans R. and Jont{\´e}, J. Michael}, title = {Polylactones, 2. Copolymerization of glycolide with \&\#946;-propiolactone, \&\#967;-butyrolactone or \&\#948;-valerolactone / Kricheldorf, Hans R. ; Mang, Thomas ; Jont{\´e}, J. Michael}, series = {Die makromolekulare Chemie. 186 (1985), H. 5}, journal = {Die makromolekulare Chemie. 186 (1985), H. 5}, isbn = {1022-1352}, pages = {955 -- 976}, year = {1985}, language = {en} } @article{MangKricheldorfAu1985, author = {Mang, Thomas and Kricheldorf, Hans R. and Au, M.}, title = {Models of molecular evolution. 2. Stereospecificity of dipeptide syntheses by means of cyanamides and carbodiimides / Kricheldorf, Hans R. ; Au, M. ; Mang, Thomas}, series = {International journal of peptide and protein research. 26 (1985), H. 2}, journal = {International journal of peptide and protein research. 26 (1985), H. 2}, isbn = {0300-9769}, pages = {149 -- 157}, year = {1985}, language = {en} } @article{MangHaulena1992, author = {Mang, Thomas and Haulena, Friedhelm}, title = {Monodisperse latex particles with functional groups}, series = {Modification of polymers : 4th Dresden Polymer Discussion ; September 22 to 25, 1992 at Gaussig / Dresden University of Technology, Department of Macromolecular Chemistry and Textile Chemistry; Institute of Polymer Technology Dresden. [Organizers: Claudia Kummerl{\"o}we ...]}, journal = {Modification of polymers : 4th Dresden Polymer Discussion ; September 22 to 25, 1992 at Gaussig / Dresden University of Technology, Department of Macromolecular Chemistry and Textile Chemistry; Institute of Polymer Technology Dresden. [Organizers: Claudia Kummerl{\"o}we ...]}, publisher = {Univ.}, address = {Dresden}, pages = {68 -- 88}, year = {1992}, language = {en} } @article{MangKricheldorf1981, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {13-C-NMR Sequence Analysis 20. Stereospecificity of the polymerization of D,L-Leu-NCA and D,L-Val-NCA / Kricheldorf, Hans R. ; Mang, Thomas}, series = {Die makromolekulare Chemie. 182 (1981), H. 11}, journal = {Die makromolekulare Chemie. 182 (1981), H. 11}, isbn = {1022-1352}, pages = {3077 -- 3098}, year = {1981}, language = {en} } @article{MangKricheldorf1982, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {13-C-NMR sequence analysis. 21. Stereoselectivity of oligopeptide syntheses / Kricheldorf, Hans R. ; Mang, Thomas}, series = {Die makromolekulare Chemie. 183 (1982), H. 9}, journal = {Die makromolekulare Chemie. 183 (1982), H. 9}, isbn = {1022-1352}, pages = {2093 -- 2111}, year = {1982}, language = {en} } @article{MangKricheldorf1982, author = {Mang, Thomas and Kricheldorf, Hans R.}, title = {13-C-NMR sequence analysis. 22. Stereoselectivity of the polymerization of D,L-leucine and D,L-valine N-thiocarboxy anhydrides / Kricheldorf, Hans R. ; Mang, Thomas}, series = {Die makromolekulare Chemie. 183 (1982), H. 9}, journal = {Die makromolekulare Chemie. 183 (1982), H. 9}, isbn = {1022-1352}, pages = {2113 -- 2129}, year = {1982}, language = {en} } @article{KotterRiekertWeyland1985, author = {Kotter, Michael and Riekert, Lothar and Weyland, Friedrich}, title = {The performance of base metal oxides on ceramic carriers as catalysts for air pollution control}, series = {International chemical engineering. 25 (1985), H. 3}, journal = {International chemical engineering. 25 (1985), H. 3}, isbn = {0020-6318}, pages = {418 -- 427}, year = {1985}, language = {en} } @article{KotterGregerIhme1984, author = {Kotter, Michael and Greger, M. and Ihme, B.}, title = {Nonequilibrium phase transitions with hysteresis in solid catalysts : the system copper-oxygen-propene / M. Greger ; B. Ihme ; M. Kotter ...}, series = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 88 (1984)}, journal = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 88 (1984)}, isbn = {0005-9021}, pages = {427 -- 433}, year = {1984}, language = {en} } @article{KotterLintzTurek1992, author = {Kotter, Michael and Lintz, Hans-G{\"u}nther and Turek, Thomas}, title = {Selective catalytic reduction of nitrogen oxide by use of the Ljungstroem air heater as reactor : a case study}, series = {Chemical engineering science . 47 (1992), H. 9-11}, journal = {Chemical engineering science . 47 (1992), H. 9-11}, isbn = {0009-2509}, pages = {2763 -- 2768}, year = {1992}, language = {en} } @article{MangHaulena1998, author = {Mang, Thomas and Haulena, Friedhelm}, title = {Recycling of Polymeric Composites}, series = {Invited and contributing lectures presented at the 38th Microsymposium on Recycling of Polymers : held in Prague, Czech Republic, July 14 - 17, 1997 / International Union of Pure and Applied Chemistry, Macromolecular Division. Symposium ed. J. Kahovec.}, journal = {Invited and contributing lectures presented at the 38th Microsymposium on Recycling of Polymers : held in Prague, Czech Republic, July 14 - 17, 1997 / International Union of Pure and Applied Chemistry, Macromolecular Division. Symposium ed. J. Kahovec.}, publisher = {Wiley-VCH}, address = {Weinheim}, pages = {147 -- 156}, year = {1998}, language = {en} } @article{BaumannGradinger1991, author = {Baumann, Marcus and Gradinger, R.}, title = {Distribution of Phytoplankton Communities in Relation to the Large Scale Hydrographical Regime in the Fram Strait 1984 / Gradinger, R. ; Baumann, M.E.M.}, series = {Marine Biology. 111 (1991), H. 2}, journal = {Marine Biology. 111 (1991), H. 2}, isbn = {0025-3162}, pages = {311 -- 321}, year = {1991}, language = {en} } @article{BaumannHircheKattneretal.1991, author = {Baumann, Marcus and Hirche, H.-J. and Kattner, G. and Gradinger, R.}, title = {Plankton distribution and the impact of copepod grazing on primary production in Fram Strait, Greenland Sea / Hirche, H.-J. ; Baumann, M.E.M. ; Kattner, G.; Gradinger, R.}, series = {Journal of Marine Systems. 2 (1991), H. 3-4}, journal = {Journal of Marine Systems. 2 (1991), H. 3-4}, isbn = {0924-7963}, pages = {477 -- 494}, year = {1991}, language = {en} } @article{BaumannThomasGleitz1992, author = {Baumann, Marcus and Thomas, D. and Gleitz, M.}, title = {Efficiency of carbon assimilation and photoacclimation in a small unicellular Chaetoceros species from the Weddel Sea (Antarctica): Influence of temperature and irridiance / Thomas, D. ; Baumann, M.E.M. ; Gleitz, M.}, series = {Journal of Experimental Marine Biology and Ecology. 157 (1992), H. 2}, journal = {Journal of Experimental Marine Biology and Ecology. 157 (1992), H. 2}, isbn = {0022-0981}, pages = {195 -- 209}, year = {1992}, language = {en} } @article{BaumannBrandiniStaubes1994, author = {Baumann, Marcus and Brandini, Frederico and Staubes, Regina}, title = {The influence of light and temperature on carbonspecific DMS release by cultures of Phaeocystis antarctica and three antarctic diatoms / Baumann, Marcus E.M. ; Brandini, Frederico ; Staubes, Regina}, series = {Marine Chemistry. 45 (1994), H. 1-2}, journal = {Marine Chemistry. 45 (1994), H. 1-2}, isbn = {0304-4203}, pages = {129 -- 136}, year = {1994}, language = {en} } @article{BaumannLancelotBrandinietal.1994, author = {Baumann, Marcus and Lancelot, C. and Brandini, F. and Sakshaug, E.}, title = {The taxonomic identity of the cosmopolitan prymnesiophyte Phaeocystis, a morphological and ecophysiological approach / Baumann, M.E.M. ; Lancelot, C. ; Brandini, F. ; Sakshaug, E. ; John M.}, series = {Journal of Marine Systems. 5 (1994), H. 1}, journal = {Journal of Marine Systems. 5 (1994), H. 1}, isbn = {0924-7963}, pages = {5 -- 22}, year = {1994}, language = {en} } @article{BaumannRousseauVaulotetal.1994, author = {Baumann, Marcus and Rousseau, V. and Vaulot, D. and Casotti, R.}, title = {The life cycle of Phaeocystis (Prymnesiophycae): evidence and hypotheses / Rousseau, V. ; Vaulot, D. ; Casotti, R. ; Cariou, V. ; Lenz, J. ; Gunkel, J. ; Baumann, M.}, series = {Journal of Marine Systems. 5 (1994), H. 1}, journal = {Journal of Marine Systems. 5 (1994), H. 1}, isbn = {0924-7963}, pages = {23 -- 39}, year = {1994}, language = {en} } @article{BaumannLaraHubbertenetal.1997, author = {Baumann, Marcus and Lara, R.J. and Hubberten, D.N. and Thomas, D.N.}, title = {Dissolved organic matter studies in enclosed systems: Application of hydophobic fractionation for the assessment of organic nitrogen dynamics / Lara, R.J. ; Hubberten, U. ; Thomas, D.N. ; Baumann, M.E.M. ; Kattner, G.}, series = {Journal of Marine Systems. 13 (1997), H. 1-4}, journal = {Journal of Marine Systems. 13 (1997), H. 1-4}, isbn = {0924-7963}, pages = {155 -- 161}, year = {1997}, language = {en} } @article{BaumannGoeyensSemenehetal.1998, author = {Baumann, Marcus and Goeyens, L. and Semeneh, M. and Elskens, M.}, title = {Phytoplankton nutrient utilisation and nutrient signature in the Southern Ocean / Goeyens, L. ; Semeneh, M. ; Baumann, M.E.M. ; Elskens, M. ; Shopova, D. ; Dehairs, F.}, series = {Journal of Marine Systems. 17 (1998), H. 1-4}, journal = {Journal of Marine Systems. 17 (1998), H. 1-4}, isbn = {0924-7963}, pages = {143 -- 157}, year = {1998}, language = {en} } @article{BaumannSemenehDehairsetal.1998, author = {Baumann, Marcus and Semeneh, M. and Dehairs, F. and Elskens, M.}, title = {Nitrogen uptake regime and phytoplankton community structure in the Southern Ocean / Semeneh, M. ; Dehairs, F. ; Elskens, M. ; Baumann, M.E.M. ; Kopczynska, E.E. ; Lancelot, C. ; Goeyens, L.}, series = {Journal of Marine Systems. 17 (1998), H. 1-4}, journal = {Journal of Marine Systems. 17 (1998), H. 1-4}, isbn = {0924-7963}, pages = {159 -- 177}, year = {1998}, language = {en} } @article{BaumannJahnke1983, author = {Baumann, Marcus and Jahnke, J.}, title = {Chemical and Physical Effects on the Shape and Growth of the Diatom Biddulphia sinensis GREVILLE in Batch Cultures: A Contribution to Bioindication in Plankton Ecology / Jahnke, J. ; Baumann, M.}, series = {Hydrobiological Bulletin . 17 (1983), H. 1}, journal = {Hydrobiological Bulletin . 17 (1983), H. 1}, isbn = {0165-1404}, pages = {5 -- 20}, year = {1983}, language = {en} } @article{BaumannTillmannAletsee1989, author = {Baumann, Marcus and Tillmann, U. and Aletsee, L.}, title = {Distribution of Carbon Among Photosynthetic End Products in the Bloom-Forming Arctic Diatom Thalassiosira antarctica COMBER / Tillmann, U. ; Baumann, M.E.M. ; Aletsee, L.}, series = {Polar Biology. 10 (1989), H. 3}, journal = {Polar Biology. 10 (1989), H. 3}, isbn = {0722-4060}, pages = {231 -- 238}, year = {1989}, language = {en} } @article{MeyerStorkHoeckerBerndt1992, author = {Meyer-Stork, L. Sebastian and H{\"o}cker, Hartwig and Berndt, Heinz}, title = {Syntheses and reactions of urethanes of cellobiose and cellulose-containing uretdione groups}, series = {Journal of applied polymer science}, volume = {44}, journal = {Journal of applied polymer science}, number = {6}, issn = {1097-4628}, pages = {1043 -- 1049}, year = {1992}, language = {en} } @article{WincklerKruegerSchnitzleretal.2014, author = {Winckler, Silvia and Krueger, Rolf and Schnitzler, Thomas and Zang, Werner and Fischer, Rainer and Biselli, Manfred}, title = {A sensitive monitoring system for mammalian cell cultivation processes: a PAT approach}, series = {Bioprocess and biosystems engineering}, volume = {37}, journal = {Bioprocess and biosystems engineering}, number = {5}, publisher = {Springer}, address = {Berlin, Heidelberg}, issn = {1615-7591 (Print) 1615-7605 (Online)}, doi = {10.1007/s00449-013-1062-8}, pages = {901 -- 912}, year = {2014}, abstract = {Biopharmaceuticals such as antibodies are produced in cultivated mammalian cells, which must be monitored to comply with good manufacturing practice. We, therefore, developed a fully automated system comprising a specific exhaust gas analyzer, inline analytics and a corresponding algorithm to precisely determine the oxygen uptake rate, carbon dioxide evolution rate, carbon dioxide transfer rate, transfer quotient and respiratory quotient without interrupting the ongoing cultivation, in order to assess its reproducibility. The system was verified using chemical simulation experiments and was able to measure the respiratory activity of hybridoma cells and DG44 cells (derived from Chinese hamster ovary cells) with satisfactory results at a minimum viable cell density of ~2.0 × 10⁵ cells ml⁻¹. The system was suitable for both batch and fed-batch cultivations in bubble-aerated and membrane-aerated reactors, with and without the control of pH and dissolved oxygen.}, language = {en} } @article{Schnitzler2009, author = {Schnitzler, Thomas}, title = {Cultivation of hybridoma cell line CF-10H5 (DSMZ ACC477)}, series = {Application notes / Sartorius stedim biotech}, journal = {Application notes / Sartorius stedim biotech}, pages = {1 -- 4}, year = {2009}, language = {en} } @article{HemmerlingMerschenzQuackWunderlich1988, author = {Hemmerling, H.-J. and Merschenz-Quack, Angelika and Wunderlich, H.}, title = {Crystal structures of indeno[1,2-d]imidazoles. XIth European Crystallographic Meeting, Vienna 1988}, series = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, volume = {185}, journal = {Zeitschrift f{\"u}r Kristallographie - Crystalline Materials}, number = {H. 1-4}, issn = {2196-7105 (E-Books); 2194-4946 (Print)}, pages = {256}, year = {1988}, language = {en} } @article{GerigkBujnickiGanpoNkwenkwaetal.2002, author = {Gerigk, M. and Bujnicki, R. and Ganpo-Nkwenkwa, E. and Bongaerts, Johannes and Sprenger, G. and Takors, Ralf}, title = {Process control for enhanced L-phenylalanine production using different recombinant Escherichia coli strains}, series = {Biotechnology and bioengineering}, volume = {Vol. 80}, journal = {Biotechnology and bioengineering}, number = {Iss. 7}, issn = {1097-0290 (E-Journal); 0006-3592 (Print)}, pages = {746 -- 754}, year = {2002}, language = {en} } @article{GerigkMaassKreutzeretal.2002, author = {Gerigk, M. and Maaß, D. and Kreutzer, A. and Sprenger, G. and Bongaerts, Johannes and Wubbolts, Marcel and Takors, Ralf}, title = {Enhanced pilot-scale fed-batch L-phenylalanine production with recombinant Escherichia coli by fully integrated reactive extraction}, series = {Bioprocess and biosystems engineering}, volume = {Vol. 25}, journal = {Bioprocess and biosystems engineering}, number = {Iss. 1}, issn = {1432-0797 (E-Journal); 1615-7605 (E-Journal); 0178-515X (Print); 1615-7591 (Print)}, pages = {43 -- 52}, year = {2002}, language = {en} } @article{KraemerBongaertsBovenbergetal.2003, author = {Kr{\"a}mer, Marco and Bongaerts, Johannes and Bovenberg, Roel and Kremer, Susanne and M{\"u}ller, Ulrike and Orf, Sonja and Wubbolts, Marcel and Raeven, Leon}, title = {Metabolic engineering for microbial production of shikimic acid}, series = {Metabolic engineering}, volume = {Vol. 5}, journal = {Metabolic engineering}, number = {Iss. 4}, issn = {1096-7184 (E-Journal); 1096-7176 (Print)}, pages = {277 -- 283}, year = {2003}, language = {en} } @article{PolenKraemerBongaertsetal.2005, author = {Polen, T. and Kr{\"a}mer, Marco and Bongaerts, Johannes and Wubbolts, Marcel and Wendisch, V. F.}, title = {The global gene expression response of Escherichia coli to L-phenylalanine}, series = {Journal of biotechnology}, volume = {Vol. 115}, journal = {Journal of biotechnology}, number = {Iss. 3}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, pages = {221 -- 237}, year = {2005}, language = {en} } @article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @article{DeppeBongaertsO'Connelletal.2011, author = {Deppe, Veronika Maria and Bongaerts, Johannes and O'Connell, Timothy and Maurer, Karl-Heinz and Meinhardt, Friedhelm}, title = {Enzymatic deglycation of Amadori products in bacteria}, series = {Applied microbiology and biotechnology}, volume = {Vol. 90}, journal = {Applied microbiology and biotechnology}, number = {Iss. 2}, publisher = {Springer}, address = {Berlin}, issn = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)}, pages = {399 -- 406}, year = {2011}, language = {en} } @article{DeppeKlatteBongaertsetal.2011, author = {Deppe, Veronika Maria and Klatte, Stephanie and Bongaerts, Johannes and Maurer, Karl-Heinz and O'Connell, Timothy and Meinhardt, Friedhelm}, title = {Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR}, series = {Applied and environmental microbiology}, volume = {Vol. 77}, journal = {Applied and environmental microbiology}, number = {No. 9}, publisher = {American Society of Mechanical Engineers (ASME)}, address = {New York}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, pages = {2839 -- 2846}, year = {2011}, language = {en} } @article{BongaertsEsserLorbachetal.2011, author = {Bongaerts, Johannes and Esser, Simon and Lorbach, Volker and Al-Momani, L{\´o}ay and M{\"u}ller, Michael A. and Franke, Dirk and Grondal, Christoph and Kurutsch, Anja and Bujnicki, Robert and Takors, Ralf and Raeven, Leon and Wubbolts, Marcel and Bovenberg, Roel and Nieger, Martin and Sch{\"u}rmann, Melanie and Trachtmann, Natalie and Kozak, Stefan and Sprenger, Georg A. and M{\"u}ller, Michael}, title = {Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis}, series = {Angewandte Chemie International Edition}, volume = {Vol. 50}, journal = {Angewandte Chemie International Edition}, number = {Iss. 34}, publisher = {Wiley}, address = {Weinheim}, issn = {1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)}, pages = {7781 -- 7786}, year = {2011}, language = {en} } @article{BorgmeierBongaertsMeinhardt2012, author = {Borgmeier, Claudia and Bongaerts, Johannes and Meinhardt, Friedhelm}, title = {Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production}, series = {Journal of biotechnology}, volume = {159}, journal = {Journal of biotechnology}, number = {1-2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2012.02.011}, pages = {12 -- 20}, year = {2012}, abstract = {Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele - known to cause hypersecretion in Bacillus subtilis - resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG - known to interfere with DegU DNA-binding in B. subtilis - did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.}, language = {en} } @article{ScheeleOertelBongaertsetal.2013, author = {Scheele, Sandra and Oertel, Dan and Bongaerts, Johannes and Evers, Stefan and Hellmuth, Hendrik and Maurer, Karl-Heinz and Bott, Michael and Freudl, Roland}, title = {Secretory production of an FAD cofactor-containing cytosolic enzyme (sorbitol-xylitol oxidase from Streptomyces coelicolor) using the twin-arginine translocation (Tat) pathway of Corynebacterium glutamicum}, series = {Microbial biotechnology}, journal = {Microbial biotechnology}, publisher = {Wiley-Blackwell}, address = {Oxford}, issn = {1751-7915}, pages = {202 -- 206}, year = {2013}, language = {en} } @article{WilmingBegemannKuhneetal.2013, author = {Wilming, Anja and Begemann, Jens and Kuhne, Stefan and Regestein, Lars and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and B{\"u}chs, Jochen}, title = {Metabolic studies of γ-polyglutamic acid production in Bacillus licheniformis by small-scale continuous cultivations}, series = {Biochemical engineering journal}, volume = {Vol. 73}, journal = {Biochemical engineering journal}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-295X (E-Journal); 1369-703X (Print)}, pages = {29 -- 37}, year = {2013}, language = {en} } @article{VoigtSchroeterJuergenetal.2013, author = {Voigt, Birgit and Schroeter, Rebecca and J{\"u}rgen, Britta and Albrecht, Dirk and Evers, Stefan and Bongaerts, Johannes and Maurer, Karl-Heinz and Schweder, Thomas and Hecker, Michael}, title = {The response of Bacillus licheniformis to heat and ethanol stress and the role of the SigB regulon}, series = {Proteomics}, volume = {Vol. 13}, journal = {Proteomics}, number = {Iss. 14}, publisher = {Wiley}, address = {Weinheim}, issn = {1615-9861 (E-Journal); 1615-9853 (Print)}, pages = {2140 -- 2146}, year = {2013}, language = {en} } @article{RachingerBauchStrittmatteretal.2013, author = {Rachinger, Michael and Bauch, Melanie and Strittmatter, Axel and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Daniel, Rolf and Liebl, Wolfgang and Liesegang, Heiko and Ehrenreich, Armin}, title = {Size unlimited markerless deletions by a transconjugative plasmid-system in Bacillus licheniformis}, series = {Journal of biotechnology}, volume = {Vol. 164}, journal = {Journal of biotechnology}, number = {Iss. 4}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, pages = {365 -- 369}, year = {2013}, language = {en} } @article{WiegandDietrichHerteletal.2013, author = {Wiegand, Sandra and Dietrich, Sascha and Hertel, Robert and Bongaerts, Johannes and Evers, Stefan and Volland, Sonja and Daniel, Rolf and Liesegang, Heiko}, title = {RNA-Seq of Bacillus licheniformis: active regulatory RNA features expressed within a productive fermentation}, series = {BMC genomics}, volume = {Vol. 14}, journal = {BMC genomics}, publisher = {BioMed Central}, address = {London}, issn = {1471-2164}, pages = {667}, year = {2013}, language = {en} } @article{KalbeHoeckerBerndt1989, author = {Kalbe, Jochen and H{\"o}cker, Hartwig and Berndt, Heinz}, title = {Design of enzyme reactors as chromatographic columns for racemic resolution of amino acid esters}, series = {Chromatographia}, volume = {28}, journal = {Chromatographia}, number = {3-4}, isbn = {0009-5893}, doi = {10.1007/BF02319646}, pages = {193 -- 196}, year = {1989}, language = {en} } @article{KuropkaMuellerHoeckeretal.1989, author = {Kuropka, Rolf and M{\"u}ller, Bettina and H{\"o}cker, Hartwig and Berndt, Heinz}, title = {Chiral stationary phases via hydrosilylation reaction of N-acryloylamino acids : I. Stationary phase with one chiral centre for high-performance liquid chromatography and development of a new derivatization pattern for amino acid enantiomers}, series = {Journal of chromatography A}, journal = {Journal of chromatography A}, number = {481}, isbn = {0021-9673}, pages = {380 -- 386}, year = {1989}, language = {en} } @article{KalbeKuropkaMeyerStorketal.1988, author = {Kalbe, Jochen and Kuropka, Rolf and Meyer-Stork, L. Sebastian and Berndt, Heinz and [u.a.],}, title = {Isolation and characterization of high-molecular mass DNA from hair shafts}, series = {Biological chemistry}, volume = {369}, journal = {Biological chemistry}, number = {1}, isbn = {0177-3593}, doi = {10.1515/bchm3.1988.369.1.413}, pages = {413 -- 416}, year = {1988}, language = {en} } @article{TranBongaertsVladetal.1997, author = {Tran, Quang Hon and Bongaerts, Johannes and Vlad, Dorina and Unden, Gottfried}, title = {Requirement for the proton-pumping NADH dehydrogenase I of Escherichia coli in respiration of NADH to fumarate and its bioenergetic implications}, series = {European journal of biochemistry}, volume = {Vol. 244}, journal = {European journal of biochemistry}, number = {Iss. 1}, issn = {0014-2956}, pages = {155 -- 160}, year = {1997}, language = {en} } @article{UndenBongaerts1997, author = {Unden, Gottfried and Bongaerts, Johannes}, title = {Alternative respiratory pathways of Escherichia coli: energetics and transcriptional regulation in response to electron acceptors}, series = {Biochimica et biophysica acta (BBA) - Bioenergetics}, volume = {Vol. 1320}, journal = {Biochimica et biophysica acta (BBA) - Bioenergetics}, number = {Iss. 3}, issn = {1879-2650 (E-Journal); 0005-2728 (Print)}, pages = {217 -- 234}, year = {1997}, language = {en} } @article{ZientzBongaertsUnden1998, author = {Zientz, Evelyn and Bongaerts, Johannes and Unden, Gottfried}, title = {Fumarate regulation of gene expression in Escherichia coli by the DcuSR (dcuSR genes) two-component regulatory system}, series = {Journal of bacteriology}, volume = {Vol. 180}, journal = {Journal of bacteriology}, number = {No. 20}, issn = {1098-5530 (E-Journal); 0021-9193 (Print)}, pages = {5421 -- 5425}, year = {1998}, language = {en} } @article{WackwitzBongaertsGoodmanetal.1999, author = {Wackwitz, B. and Bongaerts, Johannes and Goodman, S. D. and Unden, Gottfried}, title = {Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance}, series = {Molecular and general genetics : MGG}, volume = {Vol. 262}, journal = {Molecular and general genetics : MGG}, number = {Iss. 4 - 5}, issn = {1617-4623 (E-Journal); 1617-4615 (Print)}, pages = {876 -- 883}, year = {1999}, language = {en} } @article{MuellerBongaertsBovenbergetal.2001, author = {M{\"u}ller, Ulrike and Bongaerts, Johannes and Bovenberg, Roel and Jossek, Ralf and Kr{\"a}mer, Marco and Linnemann, J. and M{\"u}schen, S. and Ritterbecks, S. and Sprenger, G. and Wubbolts, Marcel}, title = {Metabolic engineering to produce fine chemicals in Escherichia coli}, series = {Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent}, volume = {66 (3a)}, journal = {Mededelingen van de Faculteit Landbouwwetenschappen, Rijksuniversiteit Gent}, issn = {0035-533x}, pages = {215 -- 217}, year = {2001}, language = {en} } @article{JossekBongaertsSprenger2001, author = {Jossek, Ralf and Bongaerts, Johannes and Sprenger, Georg A.}, title = {Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli}, series = {FEMS microbiology letters}, volume = {Vol. 202}, journal = {FEMS microbiology letters}, number = {Iss. 1}, issn = {1574-6968}, pages = {145 -- 148}, year = {2001}, language = {en} } @article{BongaertsKraemerMuelleretal.2001, author = {Bongaerts, Johannes and Kr{\"a}mer, Marco and M{\"u}ller, Ulrike and Raeven, Leon and Wubbolts, Marcel}, title = {Metabolic engineering for microbial production of aromatic amino acids and derived compounds}, series = {Metabolic engineering}, volume = {Vol. 3}, journal = {Metabolic engineering}, number = {Iss. 4}, issn = {1096-7184 (E-Journal); 1096-7176 (Print)}, pages = {289 -- 300}, year = {2001}, language = {en} } @article{SchmitzHirschBongaertsetal.2002, author = {Schmitz, M. and Hirsch, E. and Bongaerts, Johannes and Takors, Ralf}, title = {Pulse experiments as a prerequisite for the quantification of in vivo enzyme kinetics in aromatic amino acid pathway of Eschericia coli}, series = {Biotechnology progress}, volume = {Vol. 18}, journal = {Biotechnology progress}, number = {Iss. 5}, issn = {1520-6033 (E-Journal); 8756-7938 (Print)}, pages = {935 -- 941}, year = {2002}, language = {en} } @article{BongaertsBovenbergKraemeretal.2002, author = {Bongaerts, Johannes and Bovenberg, Roel and Kr{\"a}mer, Marco and M{\"u}ller, Ulrike and Raeven, Leon and Wubbolts, Marcel}, title = {Metabolic engineering to produce fine chemicals in Escherichia coli}, series = {Chemie - Ingenieur - Technik (CIT)}, volume = {Vol. 74}, journal = {Chemie - Ingenieur - Technik (CIT)}, number = {Iss. 5}, issn = {1522-2640 (E-Journal); 0009-286X (Print)}, pages = {694}, year = {2002}, language = {en} } @article{KruegerGroetzingerBerndt1987, author = {Kr{\"u}ger, G{\"o}tz and Gr{\"o}tzinger, Joachim and Berndt, Heinz}, title = {Enantiomeric resolution of amino acid derivatives on chiral stationary phases by high-performance liquid chromatography}, series = {Journal of Chromatography A}, volume = {1987}, journal = {Journal of Chromatography A}, number = {397}, issn = {0021-9673}, doi = {10.1016/S0021-9673(01)85005-6}, pages = {223 -- 232}, year = {1987}, language = {en} } @article{HeinzeMangPeteretal.2014, author = {Heinze, Daniel and Mang, Thomas and Peter, Karin and M{\"o}ller, Martin and Weichold, Oliver}, title = {Synthesis of low molecular weight poly(vinyl acetate) and its application as plasticizer}, series = {Journal of applied polymer science}, volume = {131}, journal = {Journal of applied polymer science}, number = {9}, publisher = {Wiley}, address = {New York}, issn = {1097-4628 (E-Journal); 0021-8995 (Print)}, doi = {10.1002/app.40226}, pages = {Article No. 40226}, year = {2014}, abstract = {Poly(vinyl acetate), PVAc, with a degree of polymerization Xn = 10 was prepared by chain-transfer radical polymerization using carbon tetrachloride and used as oligomeric plasticizer for commercial PVAc. However, the chlorinated chain ends cause a low thermal stability requiring mild Cl/H substitution. The product exhibits high thermal stability and excellent melt-compounding properties. Blends of oligomeric and commercial PVAc show single glass transition temperatures which decrease with higher oligomer content and exhibit small negative deviations from Fox' linear additivity rule. This indicates plasticization and miscibility being mainly due to entropic effects. Injection-moulded thick specimens show ductile behaviour at oligomer contents >10 wt \%, while sheets with a thickness of 0.2-0.5 mm appear flexible already at 7.5 wt \%. The oxygen permeability coefficients are an order of magnitude lower than those of low-density polyethylene. Due to the sum of their properties, the plasticized sheets present a promising alternative in the preparation of barrier materials.}, language = {en} } @article{UndenBeckerBongaertsetal.1994, author = {Unden, G. and Becker, S. and Bongaerts, Johannes and Schirawski, J. and Six, S.}, title = {Oxygen regulated gene expression in facultatively anaerobic bacteria}, series = {Antonie van Leeuwenhoek}, volume = {Vol. 66}, journal = {Antonie van Leeuwenhoek}, number = {Iss. 1-3}, issn = {0003-6072 (Print) ; 1572-9699 (online)}, pages = {3 -- 22}, year = {1994}, language = {en} } @article{BongaertsZoschkeWeidneretal.1995, author = {Bongaerts, Johannes and Zoschke, Sascha and Weidner, Uwe and Linden, Gottfried}, title = {Transcriptional regulation of the proton translocating NADH}, series = {Molecular microbiology}, volume = {Vol. 16}, journal = {Molecular microbiology}, number = {Iss. 3}, issn = {1365-2958 (E-Journal); 0950-382x (Print)}, pages = {521 -- 534}, year = {1995}, language = {en} } @article{UndenBeckerBongaertsetal.1995, author = {Unden, G. and Becker, S. and Bongaerts, Johannes and Holighaus, G. and Schirawski, J. and Six, S.}, title = {O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria}, series = {Archives of microbiology}, volume = {Vol. 164}, journal = {Archives of microbiology}, number = {Iss. 2}, issn = {1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)}, pages = {81 -- 90}, year = {1995}, language = {en} } @article{WissenbachSixBongaertsetal.1995, author = {Wissenbach, U. and Six, S. and Bongaerts, Johannes and Ternes, D. and Steinwachs, S. and Unden, G.}, title = {A third periplasmic transport system for l-arginine in Escherichia coli: molecular characterization of the artPIQMJ genes, arginine binding and transport}, series = {Molecular microbiology}, volume = {Vol. 17}, journal = {Molecular microbiology}, number = {Iss. 4}, issn = {1365-2958 (E-Journal); 0950-382x (Print)}, pages = {675 -- 686}, year = {1995}, language = {en} } @article{NaithaniKlostermeyerLangeetal.1971, author = {Naithani, V. K and Klostermeyer, Henning and Lange, H. R. and [u.a.], and Berndt, Heinz and [u.a.],}, title = {Preparation of peptide derivatives for porcine proinsulin-synthesis}, series = {Biological Chemistry}, volume = {352}, journal = {Biological Chemistry}, number = {1}, publisher = {De Gruyter}, issn = {1437-4315}, doi = {10.1515/bchm2.1971.352.1.1}, pages = {2 -- 3}, year = {1971}, language = {en} } @article{MatoniBerndt1980, author = {Matoni, Georg and Berndt, Heinz}, title = {Thermal synthesis of the optical pure pentapeptide derivative Z-(L)-Ala-(L)-Phe-Gly-(L)-Phe-Gly-OMe}, series = {Tetrahedron letters}, volume = {21}, journal = {Tetrahedron letters}, number = {1}, issn = {0040-4039}, doi = {10.1016/S0040-4039(00)93618-9}, pages = {37 -- 40}, year = {1980}, language = {en} } @article{NokiharaBerndt1978, author = {Nokihara, Kiyoshi and Berndt, Heinz}, title = {Studies on sulfur-containing peptides : tert-butyloxycarbonylsulfenyl and benzyloxycarbonylsulfenyl derivatives as protecting groups for cysteine}, series = {The journal of organic chemistry}, volume = {43}, journal = {The journal of organic chemistry}, number = {25}, publisher = {American Chemical Society}, address = {Washington}, issn = {0022-3263}, doi = {10.1021/jo00419a046}, pages = {4893 -- 4895}, year = {1978}, language = {en} } @article{NokiharaBerndt1978, author = {Nokihara, Kiyoshi and Berndt, Heinz}, title = {Synthesis of hapten-polypeptide conjugates as antigen models for the N-terminal region of the α-2-chain of rabbit skin collagen}, series = {Journal of the Royal Society of Chemistry: Perkin Transactions 1}, volume = {1978}, journal = {Journal of the Royal Society of Chemistry: Perkin Transactions 1}, number = {3}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {1364-5463}, doi = {10.1039/P19780000260}, pages = {260 -- 263}, year = {1978}, abstract = {Synthesis of derivatives of the peptide sequence L-pyroglutamyl-L-phenylalanyl-L-aspartyl-glycyl-L-lysyl-glycyl-glycyl-glycine as the antigenic determinant representing the N-terminal non-helical region of the α-2-chain of rabbit skin collagen, and conjugation to two different polypeptide carriers, are described.}, language = {en} } @article{DuttaHartkopfFroederWitteetal.2013, author = {Dutta, Suryendu and Hartkopf-Fr{\"o}der, Christoph and Witte, Karin and Brocke, Rainer and Mann, Ulrich}, title = {Molecular characterization of fossil palynomorphs by transmission micro-FTIR spectroscopy: implications for hydrocarbon source evaluation}, series = {International journal of coal geology}, volume = {Vol. 115}, journal = {International journal of coal geology}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1872-7840 (E-Journal); 0166-5162 (Print)}, pages = {13 -- 23}, year = {2013}, language = {en} } @article{BerndtKrueger1985, author = {Berndt, Heinz and Kr{\"u}ger, G{\"o}tz}, title = {Resolution of enantiomeric amino acid derivatives by high-performance liquid chromatography on chiral stationary phases}, series = {Journal of chromatography A}, volume = {1985}, journal = {Journal of chromatography A}, number = {348}, issn = {0021-9673}, doi = {10.1016/S0021-9673(01)92461-6}, pages = {275 -- 279}, year = {1985}, language = {en} } @article{DanhoNaithaniSasakietal.1980, author = {Danho, Waleed and Naithani, Vinod K. and Sasaki, Andr{\´e} N. and F{\"o}hles, Joseph and Berndt, Heinz and [u.a.],}, title = {Human proinsulin, VII : synthesis of two protected peptides corresponding to the sequences 1—45 and 46—86 of the prohormone}, series = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, volume = {361}, journal = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, number = {1}, issn = {1437-4315}, doi = {10.1515/bchm2.1980.361.1.857}, pages = {857 -- 863}, year = {1980}, language = {en} } @article{WangDruckenmuellerElbersetal.2014, author = {Wang, Ren-Qi and Druckenm{\"u}ller, Katharina and Elbers, Gereon and Guenther, Klaus and Crou{\´e}, Jean-Philippe}, title = {Analysis of aquatic-phase natural organic matter by optimized LDI-MS method}, series = {Journal of mass spectrometry}, volume = {49}, journal = {Journal of mass spectrometry}, number = {2}, publisher = {Wiley}, address = {Bognor Regis}, issn = {1096-9888}, doi = {10.1002/jms.3321}, pages = {154 -- 160}, year = {2014}, abstract = {The composition and physiochemical properties of aquatic-phase natural organic matter (NOM) are most important problems for both environmental studies and water industry. Laser desorption/ionization (LDI) mass spectrometry facilitated successful examinations of NOM, as humic and fulvic acids in NOM are readily ionized by the nitrogen laser. In this study, hydrophobic NOMs (HPO NOMs) from river, reservoir and waste water were characterized by this technique. The effect of analytical variables like concentration, solvent composition and laser energy was investigated. The exact masses of small molecular NOM moieties in the range of 200-1200 m/z were determined in reflectron mode. In addition, spectra of post-source-decay experiments in this range showed that some compounds from different natural NOMs had the same fragmental ions. In the large mass range of 1200-15 000 Da, macromolecules and their aggregates were found in HPO NOMs from natural waters. Highly humic HPO exhibited mass peaks larger than 8000 Da. On the other hand, the waste water and reservoir water mainly had relatively smaller molecules of about 2000 Da. The LDI-MS measurements indicated that highly humic river waters were able to form large aggregates and membrane foulants, while the HPO NOMs from waste water and reservoir water were unlikely to form large aggregates. Copyright © 2014 John Wiley \& Sons, Ltd.}, language = {en} } @article{SchroeterHoffmannVoigtetal.2014, author = {Schroeter, Rebecca and Hoffmann, Tamara and Voigt, Birgit and Meyer, Hanna and Bleisteiner, Monika and Muntel, Jan and J{\"u}rgen, Britta and Albrecht, Dirk and Becher, D{\"o}rte and Lalk, Michael and Evers, Stefan and Bongaerts, Johannes and Maurer, Karl-Heinz and Putzer, Harald and Hecker, Michael and Schweder, Thomas and Bremer, Erhard}, title = {Stress responses of the industrial workhorse Bacillus licheniformis to osmotic challenges}, series = {PLoS ONE}, volume = {8}, journal = {PLoS ONE}, number = {11}, publisher = {PLOS}, address = {San Francisco}, issn = {1932-6203}, doi = {10.1371/journal.pone.0080956}, pages = {e80956}, year = {2014}, abstract = {The Gram-positive endospore-forming bacterium Bacillus licheniformis can be found widely in nature and it is exploited in industrial processes for the manufacturing of antibiotics, specialty chemicals, and enzymes. Both in its varied natural habitats and in industrial settings, B. licheniformis cells will be exposed to increases in the external osmolarity, conditions that trigger water efflux, impair turgor, cause the cessation of growth, and negatively affect the productivity of cell factories in biotechnological processes. We have taken here both systems-wide and targeted physiological approaches to unravel the core of the osmostress responses of B. licheniformis. Cells were suddenly subjected to an osmotic upshift of considerable magnitude (with 1 M NaCl), and their transcriptional profile was then recorded in a time-resolved fashion on a genome-wide scale. A bioinformatics cluster analysis was used to group the osmotically up-regulated genes into categories that are functionally associated with the synthesis and import of osmostress-relieving compounds (compatible solutes), the SigB-controlled general stress response, and genes whose functional annotation suggests that salt stress triggers secondary oxidative stress responses in B. licheniformis. The data set focusing on the transcriptional profile of B. licheniformis was enriched by proteomics aimed at identifying those proteins that were accumulated by the cells through increased biosynthesis in response to osmotic stress. Furthermore, these global approaches were augmented by a set of experiments that addressed the synthesis of the compatible solutes proline and glycine betaine and assessed the growth-enhancing effects of various osmoprotectants. Combined, our data provide a blueprint of the cellular adjustment processes of B. licheniformis to both sudden and sustained osmotic stress.}, language = {en} } @article{HeineHerrmannSelmeretal.2014, author = {Heine, A. and Herrmann, G. and Selmer, Thorsten and Terwesten, F. and Buckel, W. and Reuter, K.}, title = {High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily}, series = {Proteins}, volume = {82}, journal = {Proteins}, number = {9}, publisher = {Wiley-Liss}, address = {New York}, issn = {1097-0134 (E-Journal); 0887-3585 (Print)}, doi = {10.1002/prot.24557}, pages = {2041 -- 2053}, year = {2014}, abstract = {Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 {\AA} as well as in complex with CoA at a resolution of 1.59 {\AA}. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called "hot dog fold" which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all "hot dog fold" proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041-2053. © 2014 Wiley Periodicals, Inc.}, language = {en} } @article{TakenagaBiselliSchnitzleretal.2014, author = {Takenaga, Shoko and Biselli, Manfred and Schnitzler, Thomas and {\"O}hlschl{\"a}ger, Peter and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis-Menten-like kinetics for cell culturing}, series = {Physica status solidi A : Applications and materials science}, volume = {211}, journal = {Physica status solidi A : Applications and materials science}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)}, doi = {10.1002/pssa.201330464}, pages = {1410 -- 1415}, year = {2014}, abstract = {The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17-200 mM) follows a Michaelis-Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.}, language = {en} } @article{KueppersSteffenHellmuthetal.2014, author = {K{\"u}ppers, Tobias and Steffen, Victoria and Hellmuth, Hendrik and O'Connell, Timothy and Bongaerts, Johannes and Maurer, Karl-Heinz and Wiechert, Wolfgang}, title = {Developing a new production host from a blueprint: Bacillus pumilus as an industrial enzyme producer}, series = {Microbial cell factories}, volume = {13}, journal = {Microbial cell factories}, publisher = {BioMed Central}, address = {London}, issn = {1475-2859 (E-Journal)}, doi = {10.1186/1475-2859-13-46}, pages = {Article No. 46}, year = {2014}, language = {en} } @article{GuoMiyamotoWagneretal.2014, author = {Guo, Yuanyuan and Miyamoto, Ko-ichiro and Wagner, Torsten and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Theoretical study and simulation of light-addressable potentiometric sensors}, series = {Physica status solidi (A) : applications and materials}, volume = {211}, journal = {Physica status solidi (A) : applications and materials}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0031-8965}, doi = {10.1002/pssa.201330354}, pages = {1467 -- 1472}, year = {2014}, abstract = {The light-addressable potentiometric sensor (LAPS) is a semiconductor-based potentiometric sensor using a light probe with an ability of detecting the concentration of biochemical species in a spatially resolved manner. As an important biomedical sensor, research has been conducted to improve its performance, for instance, to realize high-speed measurement. In this work, the idea of facilitating the device-level simulation, instead of using an equivalent-circuit model, is presented for detailed analysis and optimization of the performance of the LAPS. Both carrier distribution and photocurrent response have been simulated to provide new insight into both amplitude-mode and phase-mode operations of the LAPS. Various device parameters can be examined to effectively design and optimize the LAPS structures and setups for enhanced performance.}, language = {en} } @article{SmithBaumannWilsonetal.1987, author = {Smith, Walker O. and Baumann, Marcus and Wilson, David L. and Aletsee, Ludwig}, title = {Phytoplankton biomass and productivity in the marginal ice zone of the Fram Strait during summer 1984}, series = {Journal of geophysical research}, volume = {Vol. 92}, journal = {Journal of geophysical research}, number = {Iss. C7}, issn = {2156-2202 (E-Journal); 2169-9291 (E-Journal); 0148-0227 (Print); 2169-9275 (Print)}, pages = {6777 -- 6786}, year = {1987}, language = {en} } @article{JahnkeBaumann1987, author = {Jahnke, J. and Baumann, Marcus}, title = {Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel}, series = {Hydrobiological bulletin}, volume = {Vol. 21}, journal = {Hydrobiological bulletin}, number = {Iss. 2}, issn = {0165-1404 (Print); 1573-5125 (E-Journal)}, pages = {141 -- 147}, year = {1987}, language = {en} } @article{AletseeBaumann1991, author = {Aletsee, Ludwig and Baumann, Marcus}, title = {A laboratory incubator equipped with facilities to automatically simulate natural irradiance}, series = {Boletim do Instituto Oceanogr{\´a}fico / Universidade de S{\~a}o Paulo}, volume = {Vol. 39}, journal = {Boletim do Instituto Oceanogr{\´a}fico / Universidade de S{\~a}o Paulo}, number = {No. 2}, issn = {1982-436X; 0080-6331}, pages = {155 -- 159}, year = {1991}, language = {en} } @article{TuegBaumann1994, author = {T{\"u}g, Helmut and Baumann, Marcus}, title = {Problems of UV-B radiation measurements in biological research : critical remarks on current techniques and suggestions for improvements}, series = {Geophysical research letters}, volume = {Vol. 21}, journal = {Geophysical research letters}, number = {Iss. 8}, issn = {1944-8007 (E-Journal); 0094-8276 (Print)}, pages = {689 -- 692}, year = {1994}, language = {en} } @article{MedlinLangeBaumann1994, author = {Medlin, L. K. and Lange, M. and Baumann, Marcus}, title = {Genetic differentiation among three colony-forming species of Phaeocystis : further evidence for the phylogeny of the Prymnesiophyta}, series = {Phycologia}, volume = {Vol. 33}, journal = {Phycologia}, number = {Iss. 3}, issn = {0031-8884}, pages = {199 -- 212}, year = {1994}, language = {en} } @article{TuegBaumann1995, author = {T{\"u}g, Helmut and Baumann, Marcus}, title = {Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper "Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements"}, series = {Geophysical research letters}, volume = {Vol. 22}, journal = {Geophysical research letters}, number = {Iss. 9}, issn = {1944-8007 (E-Journal); 0094-8276 (Print)}, pages = {1159 -- 1160}, year = {1995}, language = {en} } @article{MartinezJakobTuetal.2013, author = {Martinez, Ronny and Jakob, Felix and Tu, Ran and Siegert, Petra and Maurer, Karl-Heinz and Schwaneberg, Ulrich}, title = {Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution}, series = {Biotechnology and bioengineering}, volume = {Vol. 110}, journal = {Biotechnology and bioengineering}, number = {Iss. 3}, publisher = {Wiley}, address = {Weinheim}, issn = {1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print)}, pages = {711 -- 720}, year = {2013}, language = {en} } @article{JakobMartinezMandaweetal.2013, author = {Jakob, Felix and Martinez, Ronny and Mandawe, John and Hellmuth, Hendrik and Siegert, Petra and Maurer, Karl-Heinz and Schwaneberg, Ulrich}, title = {Surface charge engineering of a Bacillus gibsonii subtilisin protease}, series = {Applied microbiology and biotechnology}, volume = {Vol. 97}, journal = {Applied microbiology and biotechnology}, number = {Iss. 15}, publisher = {Springer}, address = {Berlin}, issn = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)}, pages = {6793 -- 6802}, year = {2013}, language = {en} } @article{NiehausGaborWielandetal.2011, author = {Niehaus, F. and Gabor, E. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Eck, J.}, title = {Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases}, series = {Microbial biotechnology}, volume = {Vol. 4}, journal = {Microbial biotechnology}, number = {Iss. 6}, publisher = {Springer}, address = {Berlin}, issn = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)}, pages = {767 -- 776}, year = {2011}, language = {en} } @article{RibitschHeumannKarletal.2012, author = {Ribitsch, D. and Heumann, S. and Karl, W. and Gerlach, J. and Leber, R. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Siegert, Petra and Lange, J. and Maurer, Karl-Heinz and Berg, G. and Guebitz, G. M. and Schwab, H.}, title = {Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli}, series = {Journal of biotechnology}, volume = {157}, journal = {Journal of biotechnology}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2011.09.025}, pages = {140 -- 147}, year = {2012}, abstract = {A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @article{SiegertMcLeishBaumannetal.2005, author = {Siegert, Petra and McLeish, Michael J. and Baumann, Martin and Iding, Hans and Kneen, Malea M. and Kenyon, George L. and Pohl, Martina}, title = {Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida}, series = {Protein engineering, design, and selection : peds}, volume = {Vol. 18}, journal = {Protein engineering, design, and selection : peds}, number = {Iss. 7}, issn = {1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print)}, pages = {345 -- 357}, year = {2005}, language = {en} } @article{DuenkelmannKolterJungNitscheetal.2002, author = {D{\"u}nkelmann, Pascal and Kolter-Jung, Doris and Nitsche, Adam and Demir, Ayhan S. and Siegert, Petra and Lingen, Bettina and Baumann, Martin and Pohl, Martina and M{\"u}ller, Michael}, title = {Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation}, series = {Journal of the American Chemical Society}, volume = {Vol. 124}, journal = {Journal of the American Chemical Society}, issn = {1520-5126 (E-Journal); 0002-7863 (Print)}, pages = {12084 -- 12085}, year = {2002}, language = {en} } @article{DuennwaldDemirSiegertetal.2001, author = {D{\"u}nnwald, Thomas and Demir, Ayhan S. and Siegert, Petra and Pohl, Martina and M{\"u}ller, Michael}, title = {ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation}, series = {Cheminform}, volume = {Vol. 32}, journal = {Cheminform}, number = {Iss. 4}, issn = {1522-2667 (E-Journal); 0931-7597 (Print)}, pages = {Publ. online}, year = {2001}, language = {en} } @article{DuennwaldDemirSiegertetal.2000, author = {D{\"u}nnwald, Thomas and Demir, Ayhan S. and Siegert, Petra and Pohl, Martina and M{\"u}ller, Michael}, title = {Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C-C Bond Formation}, series = {European journal of organic chemistry}, volume = {Vol. 2000}, journal = {European journal of organic chemistry}, number = {Iss. 11}, issn = {0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print)}, pages = {2161 -- 2170}, year = {2000}, language = {en} } @article{IdingDuennwaldGreineretal.2000, author = {Iding, Hans and D{\"u}nnwald, Thomas and Greiner, Lasse and Liese, Andreas and M{\"u}ller, Michael and Siegert, Petra and Gr{\"o}tzinger, Joachim and Demir, Ayhan S. and Pohl, Martina}, title = {Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones}, series = {Chemistry - a European journal}, volume = {Vol. 6}, journal = {Chemistry - a European journal}, number = {Iss. 8}, issn = {1521-3765 (E-Journal); 0947-6539 (Print)}, pages = {1483 -- 1495}, year = {2000}, language = {en} } @article{WhiteheadOehlschlaegerAlmajhdietal.2014, author = {Whitehead, Mark and {\"O}hlschl{\"a}ger, Peter and Almajhdi, Fahad N. and Alloza, Leonor and Marz{\´a}bal, Pablo and Meyers, Ann E. and Hitzeroth, Inga I. and Rybicki, Edward P.}, title = {Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice}, series = {BMC cancer}, journal = {BMC cancer}, number = {14:367}, publisher = {BioMed Central}, address = {London}, issn = {1471-2407}, doi = {10.1186/1471-2407-14-367}, pages = {1 -- 15}, year = {2014}, language = {en} } @article{IdingSiegertMeschetal.1998, author = {Iding, Hans and Siegert, Petra and Mesch, K. and Pohl, Martina}, title = {Application of α-keto acid decarboxylases in biotransformations}, series = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology}, volume = {Vol. 1385}, journal = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology}, number = {Iss. 2}, issn = {1879-2588 (E-Journal); 0167-4838 (Print)}, pages = {307 -- 322}, year = {1998}, language = {en} } @article{PohlSiegertMeschetal.1998, author = {Pohl, Martina and Siegert, Petra and Mesch, K. and Bruhn, H. and Gr{\"o}tzinger, Joachim}, title = {Active site mutants of pyruvate decarboxylase from Zymomonas mobilis : a site-directed mutagenesis study of L112, I472, I476, E473 and N482}, series = {European journal of biochemistry}, volume = {Vol. 257}, journal = {European journal of biochemistry}, number = {Iss. 3}, issn = {1432-1033 (E-Journal); 1742-4658 (E-Journal); 0014-2956 (Print); 1742-464X (Print)}, pages = {538 -- 546}, year = {1998}, language = {en} } @article{RaueWambachGloeggleretal.2014, author = {Raue, Markus and Wambach, M. and Gl{\"o}ggler, S. and Grefen, Dana and Kaufmann, R. and Abetz, C. and Georgopanos, P. and Handge, U. A. and Mang, Thomas and Bl{\"u}mich, B. and Abetz, V.}, title = {Investigation of historical hard rubber ornaments of Charles Goodyear}, series = {Macromolecular chemistry and physics}, volume = {Vol. 215}, journal = {Macromolecular chemistry and physics}, number = {No. 3}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1022-1352}, pages = {245 -- 254}, year = {2014}, language = {en} } @article{SeifarthGossmannGrosseetal.2015, author = {Seifarth, Volker and Goßmann, Matthias and Grosse, J. O. and Becker, C. and Heschel, I. and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds}, series = {Urologia Internationalis}, volume = {2015}, journal = {Urologia Internationalis}, number = {95}, publisher = {Karger}, address = {Basel}, issn = {0042-1138}, doi = {10.1159/000368419}, pages = {106 -- 113}, year = {2015}, language = {en} } @article{PilasIkenSelmeretal.2015, author = {Pilas, Johanna and Iken, Heiko and Selmer, Thorsten and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Development of a multi-parameter sensor chip for the simultaneous detection of organic compounds in biogas processes}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431894}, pages = {1306 -- 1312}, year = {2015}, abstract = {An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.}, language = {en} } @article{BreuerRaueKirschbaumetal.2015, author = {Breuer, Lars and Raue, Markus and Kirschbaum, M. and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, R. and Wagner, Torsten}, title = {Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431944}, pages = {1368 -- 1374}, year = {2015}, abstract = {Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.}, language = {en} } @article{SchiffelsSelmer2015, author = {Schiffels, Johannes and Selmer, Thorsten}, title = {A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro}, series = {Biotechnology and Bioengineering}, volume = {112}, journal = {Biotechnology and Bioengineering}, number = {11}, publisher = {Wiley}, address = {Weinheim}, issn = {1097-0290}, doi = {10.1002/bit.25658}, pages = {2360 -- 2372}, year = {2015}, language = {en} } @article{VoigtAlbrechtSieversetal.2015, author = {Voigt, Birgit and Albrecht, Dirk and Sievers, Susanne and Becher, D{\"o}rte and Bongaerts, Johannes and Evers, Stefan and Schweder, Thomas and Maurer, Karl-Heinz and Hecker, Michael}, title = {High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium}, series = {Proteomics}, volume = {15}, journal = {Proteomics}, number = {15}, publisher = {Wiley}, address = {Weinheim}, issn = {1615-9861}, doi = {10.1002/pmic.201400504}, pages = {2629 -- 2633}, year = {2015}, language = {en} } @article{MolinnusBaeckerSiegertetal.2015, author = {Molinnus, Denise and B{\"a}cker, Matthias and Siegert, Petra and Willenberg, H. and Poghossian, Arshak and Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Detection of Adrenaline Based on Substrate Recycling Amplification}, series = {Procedia Engineering}, volume = {120}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2015.08.708}, pages = {540 -- 543}, year = {2015}, abstract = {An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.}, language = {en} } @article{PilasMarianoKeusgenetal.2015, author = {Pilas, Johanna and Mariano, K. and Keusgen, M. and Selmer, Thorsten and Sch{\"o}ning, Michael Josef}, title = {Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes}, series = {Procedia Engineering}, volume = {120}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2015.08.702}, pages = {532 -- 535}, year = {2015}, language = {en} }