@article{WuBronderPoghossianetal.2015, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Werner, Frederik and Sch{\"o}ning, Michael Josef}, title = {Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer}, series = {Nanoscale}, volume = {14}, journal = {Nanoscale}, number = {7}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, doi = {10.1039/C4NR07225A}, pages = {6143 -- 6150}, year = {2015}, abstract = {A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent-voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout.}, language = {en} } @article{BronderWuPoghossianetal.2014, author = {Bronder, Thomas and Wu, Chunsheng and Poghossian, Arshak and Werner, Frederik and Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Label-free detection of DNA hybridization with light-addressable potentiometric sensors: comparison of various DNA-immobilization strategies}, series = {Procedia Engineering}, volume = {87}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2014.11.647}, pages = {755 -- 758}, year = {2014}, abstract = {Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).}, language = {en} } @article{PoghossianIngebrandtAbouzaretal.2007, author = {Poghossian, Arshak and Ingebrandt, S. and Abouzar, Maryam H. and Sch{\"o}ning, Michael Josef}, title = {Label-free detection of charged macromolecules by using a field-effect-based sensor platform: Experiments and possible mechanisms of signal generation}, series = {Applied Physics A: Materials Science \& Processing. 87 (2007), H. 3}, journal = {Applied Physics A: Materials Science \& Processing. 87 (2007), H. 3}, isbn = {0947-8396}, pages = {517 -- 524}, year = {2007}, language = {en} } @article{MourzinaMaiPoghossianetal.2003, author = {Mourzina, Y. and Mai, T. and Poghossian, Arshak and Ermolenko, Y. and Yoshinobu, T. and Vlasov, Y. and Iwasaki, H. and Sch{\"o}ning, Michael Josef}, title = {K+-selective field-effect sensors as transducers for bioelectronic applications}, series = {Electrochimica Acta. 48 (2003), H. 20-22}, journal = {Electrochimica Acta. 48 (2003), H. 20-22}, isbn = {0013-4686}, pages = {3333 -- 3339}, year = {2003}, language = {en} } @article{SchoeningSaukeSteffenetal.1995, author = {Sch{\"o}ning, Michael Josef and Sauke, M. and Steffen, A. and Marso, M. (u.a.)}, title = {Ion-sensitive field-effect transistors with ultrathin Langmuir-Blodgett membranes}, series = {Sensors and Actuators B. 27 (1995), H. 1-3}, journal = {Sensors and Actuators B. 27 (1995), H. 1-3}, isbn = {0925-4005}, pages = {325 -- 328}, year = {1995}, language = {en} } @article{MourzinaYoshinobuSchubertetal.2001, author = {Mourzina, Y. G. and Yoshinobu, T. and Schubert, J. and L{\"u}th, H. and Iwasaki, H. and Sch{\"o}ning, Michael Josef}, title = {Ion-selective lightaddressable potentiometric sensor (LAPS) with chalcogenide thin film by pulsed laser deposition}, series = {Sensors and Actuators B. 80 (2001), H. 2}, journal = {Sensors and Actuators B. 80 (2001), H. 2}, isbn = {0925-4005}, pages = {136 -- 140}, year = {2001}, language = {en} } @article{SchoeningBrunsHoffmannetal.1993, author = {Sch{\"o}ning, Michael Josef and Bruns, M. and Hoffmann, W. and Hoffmann, B. (u.a.)}, title = {Iodide ion-sensitive field-effect structures}, series = {Sensors and Actuators B. 15 (1993), H. 1-3}, journal = {Sensors and Actuators B. 15 (1993), H. 1-3}, isbn = {0925-4005}, pages = {192 -- 194}, year = {1993}, language = {en} } @article{SchoeningCrottRonkeletal.1996, author = {Sch{\"o}ning, Michael Josef and Crott, M. and Ronkel, F. and Thust, M. and Schultze, J. W. and Kordos, P. and L{\"u}th, H.}, title = {Investigations on porous silicon layers with regard to chemical microsensor applications}, series = {Third International Conference on Intelligent Materials : Lyon, 3-4-5 June 1996, Center of Congress "L'Espace T{\^e}te d'Or" / [publ. by SPIE, the International Society for Optical Engineering]. General chair/ed. by: P. F. Gobin}, journal = {Third International Conference on Intelligent Materials : Lyon, 3-4-5 June 1996, Center of Congress "L'Espace T{\^e}te d'Or" / [publ. by SPIE, the International Society for Optical Engineering]. General chair/ed. by: P. F. Gobin}, publisher = {SPIE}, address = {Bellingham, Wash.}, isbn = {0819421650}, pages = {275 -- 280}, year = {1996}, language = {en} } @article{BreuerMangSchoeningetal.2017, author = {Breuer, Lars and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, Ronald and Wagner, Torsten}, title = {Investigation of the spatial resolution of a laser-based stimulation process for light-addressable hydrogels with incorporated graphene oxide by means of IR thermography}, series = {Sensors and Actuators A: Physical}, volume = {268}, journal = {Sensors and Actuators A: Physical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0924-4247}, doi = {10.1016/j.sna.2017.11.031}, pages = {126 -- 132}, year = {2017}, language = {en} } @article{IsmailHaradaYoshinobuetal.2000, author = {Ismail, A.B.M. and Harada, T. and Yoshinobu, T. and Iwasaki, H. and Sch{\"o}ning, Michael Josef and L{\"u}th, H.}, title = {Investigation of pulsed laser-deposited Al2O3 as a high pH-sensitive layer for LAPS-based biosensing applications}, series = {Sensors and Actuators B. 71 (2000), H. 3}, journal = {Sensors and Actuators B. 71 (2000), H. 3}, isbn = {0925-4005}, pages = {169 -- 172}, year = {2000}, language = {en} } @article{NaetherHenkelSchneideretal.2009, author = {N{\"a}ther, Niko and Henkel, Hartmut and Schneider, Andreas and Sch{\"o}ning, Michael Josef}, title = {Investigation of different catalytically active and passive materials for realising a hydrogen peroxide gas sensor}, series = {physica status solidi (a) . 206 (2009), H. 3}, journal = {physica status solidi (a) . 206 (2009), H. 3}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {449 -- 454}, year = {2009}, language = {en} } @article{PoghossianKraemerAbouzaretal.2009, author = {Poghossian, Arshak and Kr{\"a}mer, Melina and Abouzar, Maryam H. and Pita, Marcos and Katz, Evgeny and Sch{\"o}ning, Michael Josef}, title = {Interfacing of biocomputing systems with silicon chips: Enzyme logic gates based on field-effect devices}, series = {Procedia Chemistry. 1 (2009), H. 1}, journal = {Procedia Chemistry. 1 (2009), H. 1}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1876-6196}, pages = {682 -- 685}, year = {2009}, language = {en} } @article{PoghossianMalzahnAbouzaretal.2011, author = {Poghossian, Arshak and Malzahn, K. and Abouzar, Maryam H. and Mehndiratta, P. and Katz, E. and Sch{\"o}ning, Michael Josef}, title = {Integration of biomolecular logic gates with field-effect transducers}, series = {Electrochimica Acta. 56 (2011), H. 26}, journal = {Electrochimica Acta. 56 (2011), H. 26}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0013-4686}, pages = {9661 -- 9665}, year = {2011}, language = {en} } @article{RolkaPoghossianSchoening2004, author = {Rolka, David and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Integration of a capacitive EIS sensor into a FIA system for pH and penicillin determination}, series = {Sensors. 4 (2004)}, journal = {Sensors. 4 (2004)}, isbn = {1424-8220}, pages = {84 -- 94}, year = {2004}, language = {en} } @article{SchrothSchoeningKordosetal.1999, author = {Schroth, P. and Sch{\"o}ning, Michael Josef and Kordos, P. and L{\"u}th, H. and Sch{\"u}tz, S. and Weißbecker, B. and Hummel, Hans E.}, title = {Insectbased BioFETs with improved signal characteristics}, series = {Biosensors \& Bioelectronics. 14 (1999), H. 3}, journal = {Biosensors \& Bioelectronics. 14 (1999), H. 3}, issn = {0956-5663}, pages = {303 -- 308}, year = {1999}, language = {en} } @article{SchuetzCzynskiHummeletal.2000, author = {Sch{\"u}tz, S. and Czynski, J. and Hummel, Hans E. and Sch{\"o}ning, Michael Josef and Schroth, P. and Kordos, P. and L{\"u}th, H.}, title = {Insect meets microelectronics: A BioFET as a first step to bioelectronic microsystems}, series = {Transducers '99 : proceedings of the Tenth International Conference on Solid-State Sensors and Actuators ; 7 - 10 June, 1999, Sendai, Japan / ed.: S. Middelhoek}, journal = {Transducers '99 : proceedings of the Tenth International Conference on Solid-State Sensors and Actuators ; 7 - 10 June, 1999, Sendai, Japan / ed.: S. Middelhoek}, publisher = {Elsevier}, address = {Amsterdam [u.a.]}, pages = {1104 -- 1107}, year = {2000}, language = {en} } @article{SchoeningSchrothLuethetal.2001, author = {Sch{\"o}ning, Michael Josef and Schroth, Peter and L{\"u}th, Hans and Hummel, Hans E. and Sch{\"u}tz, Stefan}, title = {Insect chemoreceptors coupled to silicon transistors as innovative biosensors}, series = {Proceedings of SPIE. 4205 (2001)}, journal = {Proceedings of SPIE. 4205 (2001)}, pages = {152 -- 162}, year = {2001}, language = {en} } @article{KloockMourzinaErmolenkoetal.2004, author = {Kloock, Joachim P. and Mourzina, Y.G. and Ermolenko, Y. and Doll, T. and Schubert, J. and Sch{\"o}ning, Michael Josef}, title = {Inorganic thin-film sensor membranes with PLD-prepared chalcogenide glasses: Challenges and implementation}, series = {Sensors. 4 (2004), H. 10}, journal = {Sensors. 4 (2004), H. 10}, isbn = {1424-8220}, pages = {156 -- 162}, year = {2004}, language = {en} } @article{SchoeningSchubertKloocketal.2002, author = {Sch{\"o}ning, Michael Josef and Schubert, J and Kloock, Joachim P. and Zander, W. and Mourzina, Y. G. and Legin, A. and Vlasov, Y. G. and L{\"u}th, H.}, title = {Innovative thin film techniques for microfabricating electrochemical sensors}, series = {Lecture Notes of the ICB Seminars}, journal = {Lecture Notes of the ICB Seminars}, publisher = {MCB}, address = {Warsaw}, pages = {55 -- 66}, year = {2002}, language = {en} } @article{SchoeningSchubertKloocketal.2001, author = {Sch{\"o}ning, Michael Josef and Schubert, Joachim P. and Kloock, Joachim P. and Zander, W. and Mourzina, Y. G. and Legin, A. and Vlasov, Y. G. and L{\"u}th, H.}, title = {Innovative thin film techniques for microfabricating electrochemical sensors}, series = {Biocybernetics and Biomedical Engineering. 21 (2001), H. 4}, journal = {Biocybernetics and Biomedical Engineering. 21 (2001), H. 4}, isbn = {0208-5216}, pages = {107 -- 119}, year = {2001}, language = {en} }