@misc{StadtmuellerTippkoetterUlber2014, author = {Stadtm{\"u}ller, R. and Tippk{\"o}tter, Nils and Ulber, Roland}, title = {Produktion von einzelstr{\"a}ngigen DNA-Makronukleotiden}, series = {Chemie Ingenieur Technik}, volume = {86}, journal = {Chemie Ingenieur Technik}, number = {9}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0009-286X}, doi = {10.1002/cite.201450372}, pages = {1403}, year = {2014}, abstract = {In der Biotechnologie stellt Einzelstrang-DNA (ssDNA) eine Schl{\"u}sselrolle dar und fungiert z. B. als Baustein f{\"u}r die nanoskalige Feinmechanik oder als Affinit{\"a}tsligand, ein sog. Aptamer. Hinsichtlich der industriellen Verwendung bieten Aptamere im Vergleich zu Antik{\"o}rpern viele Vorteile, wie z. B. eine gute Renaturierung bzw. die Selektion f{\"u}r cytotoxische Molek{\"u}le. Aktuell w{\"a}chst die Nachfrage f{\"u}r chim{\"a}re Aptamere von bis zu 200 n, um die simultane Bindung bzw. die Modifikation mehrerer Molek{\"u}le zu realisieren. Bis heute wird ssDNA mittels einer sequentiellen Synthese hergestellt, die eine Effizienz von ca. 99,5 \% je Zyklus und bereits bei einer Produktl{\"a}nge von 100 n nur noc hAusbeuten von max. 60 \% zeigt. Um dem Bedarf an ssDNA im Bereich > 100 n zu entsprechen, wurden zwei enzymatische Verfahren zur Produktion dieser Makronukleotide entworfen. Die erste Technik basiert auf einerFestphasen-PCR und erm{\"o}glicht sowohlein Primer- als auch ein Templatrecycling. Das zweite Verfahren beruht auf einer Plasmidbasierten In-vivo-Amplifikation, der sog. AptaGENE®-Technologie. In einer einzigen Klonierung werden bis zu 100 Kopien des Monomers in einen Vektor kloniert. Nach einer Transformation folgt der regul{\"a}re Produktionsprozess in Form einer Kultivierung, Plasmidpr{\"a}paration und sequenziellen Aufarbeitung von bis zu 6 · 10¹⁵ Makronukleotiden pro Milliliter Fermentationsvolumen.}, language = {de} } @article{KluczkaEcksteinAlexopoulosetal.2014, author = {Kluczka, Sven and Eckstein, Julian and Alexopoulos, Spiros and Vaeßen, Christiane and Roeb, Martin}, title = {Process simulation for solar steam and dry reforming}, series = {Energy procedia : Proceedings of the SolarPACES 2013 International Conference}, volume = {49}, journal = {Energy procedia : Proceedings of the SolarPACES 2013 International Conference}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1876-6102 (E-Journal)}, doi = {10.1016/j.egypro.2014.03.092}, pages = {850 -- 859}, year = {2014}, abstract = {In co-operation with the German Aerospace Center, the Solar-Institut J{\"u}lich has been analyzing the different technologies that are available for methanol production from CO2 using solar energy. The aim of the project is to extract CO2 from industrial exhaust gases or directly from the atmosphere to recycle it by use of solar energy. Part of the study was the modeling and simulating of a methane reformer for the production of synthesis gas, which can be operated by solar or hybrid heat sources. The reformer has been simplified in such a way that the model is accurate and enables fast calculations. The developed pseudo-homogeneous one- dimensional model can be regarded as a kind of counter-current heat exchanger and is able to incorporate a steam reforming reaction as well as a dry reforming reaction.}, language = {en} } @inproceedings{NeugebauerBrutschyMeyeretal.2014, author = {Neugebauer, Georg and Brutschy, Lucas and Meyer, Ulrike and Wetzel, Susanne}, title = {Privacy-preserving multi-party reconciliation secure in the malicious model}, series = {DPM 2013, SETOP 2013: Data Privacy Management and Autonomous Spontaneous Security}, booktitle = {DPM 2013, SETOP 2013: Data Privacy Management and Autonomous Spontaneous Security}, editor = {Garcia-Alfaro, Joaquin and Lioudakis, Georgios and Cuppens-Boulahia, Nora and Foley, Simon and Fitzgerald, William M.}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-642-54567-2 (Print)}, doi = {10.1007/978-3-642-54568-9_12}, pages = {178 -- 193}, year = {2014}, abstract = {The problem of fair and privacy-preserving ordered set reconciliation arises in a variety of applications like auctions, e-voting, and appointment reconciliation. While several multi-party protocols have been proposed that solve this problem in the semi-honest model, there are no multi-party protocols that are secure in the malicious model so far. In this paper, we close this gap. Our newly proposed protocols are shown to be secure in the malicious model based on a variety of novel non-interactive zero-knowledge-proofs. We describe the implementation of our protocols and evaluate their performance in comparison to protocols solving the problem in the semi-honest case.}, language = {en} } @incollection{KnottSofroniaGerressenetal.2014, author = {Knott, Thomas C. and Sofronia, Raluca E. and Gerressen, Marcus and Law, Yuen and Davidescu, Arjana and Savii, George G. and Gatzweiler, Karl-Heinz and Staat, Manfred and Kuhlen, Torsten W.}, title = {Preliminary bone sawing model for a virtual reality-based training simulator of bilateral sagittal split osteotomy}, series = {Biomedical simulation : 6th International Symposium, ISBMS 2014, Strasbourg, France, October 16-17, 2014 : proceedings (Lecture notes in computer science : vol. 8789)}, booktitle = {Biomedical simulation : 6th International Symposium, ISBMS 2014, Strasbourg, France, October 16-17, 2014 : proceedings (Lecture notes in computer science : vol. 8789)}, publisher = {Springer}, address = {Cham}, isbn = {978-3-319-12057-7 (Online)}, doi = {10.1007/978-3-319-12057-7_1}, pages = {1 -- 10}, year = {2014}, abstract = {Successful bone sawing requires a high level of skill and experience, which could be gained by the use of Virtual Reality-based simulators. A key aspect of these medical simulators is realistic force feedback. The aim of this paper is to model the bone sawing process in order to develop a valid training simulator for the bilateral sagittal split osteotomy, the most often applied corrective surgery in case of a malposition of the mandible. Bone samples from a human cadaveric mandible were tested using a designed experimental system. Image processing and statistical analysis were used for the selection of four models for the bone sawing process. The results revealed a polynomial dependency between the material removal rate and the applied force. Differences between the three segments of the osteotomy line and between the cortical and cancellous bone were highlighted.}, language = {en} } @book{BirleKleinMueller2014, author = {Birle, J{\"u}rgen P. and Klein, Hartmut and M{\"u}ller, Thomas}, title = {Praxishandbuch der GmbH : Gesellschafts- und Steuerrecht}, edition = {3. Aufl.}, publisher = {NWB-Verl.}, address = {Herne}, isbn = {978-3-482-56963-0}, pages = {1105 S.}, year = {2014}, language = {de} } @inproceedings{Huening2014, author = {H{\"u}ning, Felix}, title = {Power semiconductors : key components for HEV/EV}, series = {FISITA 2014 World Automotive Congress : 2 - 6 June, Maastricht, the Netherlands International Federation of Automotive Engineering Societies}, booktitle = {FISITA 2014 World Automotive Congress : 2 - 6 June, Maastricht, the Netherlands International Federation of Automotive Engineering Societies}, publisher = {KIVI}, address = {[s.l.]}, pages = {1 USB-Speicherstick}, year = {2014}, language = {en} } @article{KuertenMottaghyZiegler2014, author = {K{\"u}rten, Sylvia and Mottaghy, Darius and Ziegler, Martin}, title = {Planung, Auslegung und Dimensionierung von thermo-aktiven Bauteilen am Beispiel thermo-aktiver Abdichtungselemente}, series = {Geothermie, Bohr- und Brunnentechnik}, journal = {Geothermie, Bohr- und Brunnentechnik}, publisher = {Ernst \& Sohn}, address = {Berlin}, pages = {18 -- 20}, year = {2014}, language = {de} } @article{BaeckerKramerHucketal.2014, author = {B{\"a}cker, Matthias and Kramer, F. and Huck, Christina and Poghossian, Arshak and Bratov, A. and Abramova, N. and Sch{\"o}ning, Michael Josef}, title = {Planar and 3D interdigitated electrodes for biosensing applications: The impact of a dielectric barrier on the sensor properties}, series = {Physica Status Solidi (A) - Applications and Materials Science}, volume = {211}, journal = {Physica Status Solidi (A) - Applications and Materials Science}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)}, doi = {10.1002/pssa.201330416}, pages = {1357 -- 1363}, year = {2014}, abstract = {Planar and three-dimensional (3D) interdigitated electrodes (IDE) with electrode digits separated by an insulating barrier of different heights were electrochemically characterized and compared in terms of their sensing properties. Due to the impact of the surface resistance, both types of IDE structures display a non-linear behavior in low-ionic strength solutions. The experimental data were fitted to an electrical equivalent circuit and interpreted taking into account the surface-charge-governed properties. The effect of a charged polyelectrolyte layer electrostatically assembled onto the sensor surface on the surface resistance in solutions with different KCl concentration is studied. In case of the same electrode footprint, 3D-IDEs show a larger cell constant and a higher sensitivity to molecular adsorption than that of planar IDEs. The obtained results demonstrate the potential of 3D-IDEs as a new transducer structure for a direct label-free sensing of charged molecules.}, language = {en} } @article{StadlerGarveyEmbsetal.2014, author = {Stadler, Alexander Maximilian and Garvey, Christopher J. and Embs, Jan Peter and Koza, Michael Marek and Unruh, Tobias and Artmann, Gerhard and Zaccai, Guiseppe}, title = {Picosecond dynamics in haemoglobin from different species: A quasielastic neutron scattering study}, series = {Biochimica et biophysica acta (BBA): General Subjects}, volume = {1840}, journal = {Biochimica et biophysica acta (BBA): General Subjects}, number = {10}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1872-8006 (E-Journal); 0304-4165 (Print)}, doi = {10.1016/j.bbagen.2014.06.007}, pages = {2989 -- 2999}, year = {2014}, language = {en} } @article{MuribYeapMartensetal.2014, author = {Murib, Mohammed Sharif and Yeap, Weng-Siang and Martens, Daan and Bienstman, Peter and Ceuninck, Ward de and Grinsven, Bart van and Sch{\"o}ning, Michael Josef and Michiels, Luc and Haenen, Ken and Ameloot, Marcel and Serpeng{\"u}zel, Ali and Wagner, Patrick}, title = {Photonic detection and characterization of DNA using sapphire microspheres}, series = {Journal of biomedical optics}, volume = {19}, journal = {Journal of biomedical optics}, number = {9}, publisher = {SPIE}, address = {Bellingham}, issn = {1560-2281 (E-Journal); 1083-3668 (Print)}, doi = {10.1117/1.JBO.19.9.097006}, pages = {097006}, year = {2014}, abstract = {A microcavity-based deoxyribonucleic acid (DNA) optical biosensor is demonstrated for the first time using synthetic sapphire for the optical cavity. Transmitted and elastic scattering intensity at 1510 nm are analyzed from a sapphire microsphere (radius 500  μm, refractive index 1.77) on an optical fiber half coupler. The 0.43 nm angular mode spacing of the resonances correlates well with the optical size of the sapphire sphere. Probe DNA consisting of a 36-mer fragment was covalently immobilized on a sapphire microsphere and hybridized with a 29-mer target DNA. Whispering gallery modes (WGMs) were monitored before the sapphire was functionalized with DNA and after it was functionalized with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA). The shift in WGMs from the surface modification with DNA was measured and correlated well with the estimated thickness of the add-on DNA layer. It is shown that ssDNA is more uniformly oriented on the sapphire surface than dsDNA. In addition, it is shown that functionalization of the sapphire spherical surface with DNA does not affect the quality factor (Q≈104) of the sapphire microspheres. The use of sapphire is especially interesting because this material is chemically resilient, biocompatible, and widely used for medical implants.}, language = {en} } @article{LagemaatVosMaasetal.2014, author = {Lagemaat, Miriam W. and Vos, Eline K. and Maas, Marnix C. and Bitz, Andreas and Orzada, Stephan and Uden, Mark J. van and Kobus, Thiele and Heerschap, Arend and Scheenen, Tom W. J.}, title = {Phosphorus magnetic resonance spectroscopic imaging at 7 T in patients with prostate cancer}, series = {Investigative Radiology}, volume = {49}, journal = {Investigative Radiology}, number = {5}, publisher = {Lippincott Williams \& Wilkins}, address = {Philadelphia, Pa.}, issn = {1536-0210}, doi = {10.1097/RLI.0000000000000012}, pages = {363 -- 372}, year = {2014}, abstract = {Objectives The aim of this study was to identify characteristics of phosphorus (³¹P) spectra of the human prostate and to investigate changes of individual phospholipid metabolites in prostate cancer through in vivo ³¹P magnetic resonance spectroscopic imaging (MRSI) at 7 T. Materials and Methods In this institutional review board-approved study, 15 patients with biopsy-proven prostate cancer underwent T₂-weighted magnetic resonance imaging and 3-dimensional ³¹P MRSI at 7 T. Voxels were selected at the tumor location, in normal-appearing peripheral zone tissue, normal-appearing transition zone tissue, and in the base of the prostate close to the seminal vesicles. Phosphorus metabolite ratios were determined and compared between tissue types. Results Signals of phosphoethanolamine (PE) and phosphocholine (PC) were present and well resolved in most ³¹P spectra in the prostate. Glycerophosphocholine signals were observable in 43\% of the voxels in malignant tissue, but in only 10\% of the voxels in normal-appearing tissue away from the seminal vesicles. In many spectra, independent of tissue type, 2 peaks resonated in the chemical shift range of inorganic phosphate, possibly representing 2 separate pH compartments. The PC/PE ratio in the seminal vesicles was highly elevated compared with the prostate in 5 patients. A considerable overlap of ³¹P metabolite ratios was found between prostate cancer and normal-appearing prostate tissue, preventing direct discrimination of these tissues. The only 2 patients with high Gleason scores tumors (≥4+5) presented with high PC and glycerophosphocholine levels in their cancer lesions. Conclusions Phosphorus MRSI at 7 T shows distinct features of phospholipid metabolites in the prostate gland and its surrounding structures. In this exploratory study, no differences in ³¹P metabolite ratios were observed between prostate cancer and normal-appearing prostate tissue possibly because of the partial volume effects of small tumor foci in large MRSI voxels.}, language = {en} } @article{ArinkinDigelPorstetal.2014, author = {Arinkin, Vladimir and Digel, Ilya and Porst, Dariusz and Temiz Artmann, Ayseg{\"u}l and Artmann, Gerhard}, title = {Phenotyping date palm varieties via leaflet cross-sectional imaging and artificial neural network application}, series = {BMC bioinformatics}, volume = {15}, journal = {BMC bioinformatics}, number = {55}, issn = {1471-2105}, doi = {10.1186/1471-2105-15-55}, pages = {1 -- 8}, year = {2014}, abstract = {Background True date palms (Phoenix dactylifera L.) are impressive trees and have served as an indispensable source of food for mankind in tropical and subtropical countries for centuries. The aim of this study is to differentiate date palm tree varieties by analysing leaflet cross sections with technical/optical methods and artificial neural networks (ANN). Results Fluorescence microscopy images of leaflet cross sections have been taken from a set of five date palm tree cultivars (Hewlat al Jouf, Khlas, Nabot Soltan, Shishi, Um Raheem). After features extraction from images, the obtained data have been fed in a multilayer perceptron ANN with backpropagation learning algorithm. Conclusions Overall, an accurate result in prediction and differentiation of date palm tree cultivars was achieved with average prediction in tenfold cross-validation is 89.1\% and reached 100\% in one of the best ANN.}, language = {en} } @article{LuisierLempiaeinenScherbichleretal.2014, author = {Luisier, Rapha{\"e}lle and Lempi{\"a}inen, Harri and Scherbichler, Nina and Braeuning, Albert and Geissler, Miriam and Dubost, Valerie and M{\"u}ller, Arne and Scheer, Nico and Chibout, Salah-Dine and Hara, Hisanori and Picard, Frank and Theil, Diethilde and Couttet, Philippe and Vitobello, Antonio and Grenet, Olivier and Grasl-Kraupp, Bettina and Ellinger-Ziegelbauer, Heidrung and Thomson, John P. and Meehan, Richard R. and Elcombe, Clifford R. and Henderson, Colin J. and Wolf, C. Roland and Schwarz, Michael and Moulin, Pierre and Terranova, Remi and Moggs, Jonathan G.}, title = {Phenobarbital Induces Cell Cycle Transcriptional Responses in Mouse Liver Humanized for Constitutive Androstane and Pregnane X Receptors}, series = {Toxicological Sciences}, volume = {139}, journal = {Toxicological Sciences}, number = {2}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1094-2025}, doi = {https://doi.org/10.1093/toxsci/kfu038}, pages = {501 -- 511}, year = {2014}, abstract = {The constitutive androstane receptor (CAR) and the pregnane X receptor (PXR) are closely related nuclear receptors involved in drug metabolism and play important roles in the mechanism of phenobarbital (PB)-induced rodent nongenotoxic hepatocarcinogenesis. Here, we have used a humanized CAR/PXR mouse model to examine potential species differences in receptor-dependent mechanisms underlying liver tissue molecular responses to PB. Early and late transcriptomic responses to sustained PB exposure were investigated in liver tissue from double knock-out CAR and PXR (CARᴷᴼ-PXRᴷᴼ), double humanized CAR and PXR (CARʰ-PXRʰ), and wild-type C57BL/6 mice. Wild-type and CARʰ-PXRʰ mouse livers exhibited temporally and quantitatively similar transcriptional responses during 91 days of PB exposure including the sustained induction of the xenobiotic response gene Cyp2b10, the Wnt signaling inhibitor Wisp1, and noncoding RNA biomarkers from the Dlk1-Dio3 locus. Transient induction of DNA replication (Hells, Mcm6, and Esco2) and mitotic genes (Ccnb2, Cdc20, and Cdk1) and the proliferation-related nuclear antigen Mki67 were observed with peak expression occurring between 1 and 7 days PB exposure. All these transcriptional responses were absent in CARᴷᴼ-PXRᴷᴼ mouse livers and largely reversible in wild-type and CARʰ-PXRʰ mouse livers following 91 days of PB exposure and a subsequent 4-week recovery period. Furthermore, PB-mediated upregulation of the noncoding RNA Meg3, which has recently been associated with cellular pluripotency, exhibited a similar dose response and perivenous hepatocyte-specific localization in both wild-type and CARʰ-PXRʰ mice. Thus, mouse livers coexpressing human CAR and PXR support both the xenobiotic metabolizing and the proliferative transcriptional responses following exposure to PB.}, language = {en} } @inproceedings{AnthrakidisHerrmannMirzaetal.2014, author = {Anthrakidis, Anette and Herrmann, Ulf and Mirza, M. F. and Schorn, Christian and Schwarzer, Klemens and Wedding, Philipp and Weis, Fabian}, title = {Parabolrinnenentwicklung und Qualifizierung am Solar-Institut J{\"u}lich}, series = {Thermische Solarenergie : 24. Symposium ; 7. bis 9. Mai 2014, Kloster Banz, Bad Staffelstein}, booktitle = {Thermische Solarenergie : 24. Symposium ; 7. bis 9. Mai 2014, Kloster Banz, Bad Staffelstein}, publisher = {OTTI}, address = {Regensburg}, organization = {Ostbayerisches Technologie-Transfer-Institut}, isbn = {978-3-943891-35-5}, pages = {242 -- 243}, year = {2014}, language = {de} } @book{SchulteZurhausen2014, author = {Schulte-Zurhausen, Manfred}, title = {Organisation}, edition = {6., {\"u}berarb. und aktualisierte Aufl.}, publisher = {Vahlen}, address = {M{\"u}nchen}, isbn = {978-3-8006-4689-0}, pages = {XVI, 687 S. : graph. Darst.}, year = {2014}, language = {de} } @article{PasteurTippkoetterKampeisetal.2014, author = {Pasteur, Aline and Tippk{\"o}tter, Nils and Kampeis, Percy and Ulber, Roland}, title = {Optimization of high gradient magnetic separation filter units for the purification of fermentation products}, series = {IEEE TRANSACTIONS ON MAGNETICS}, volume = {50}, journal = {IEEE TRANSACTIONS ON MAGNETICS}, number = {10}, publisher = {IEEE}, address = {New York, NY}, issn = {0018-9464}, doi = {10.1109/TMAG.2014.2325535}, pages = {Artikel 5000607}, year = {2014}, abstract = {High gradient magnetic separation (HGMS) has been established since the early 1970s. A more recent application of these systems is the use in bioprocesses. To integrate the HGMS in a fermentation process, it is necessary to optimize the separation matrix with regard to the magnetic separation characteristics and permeability of the non-magnetizable components of the fermentation broth. As part of the work presented here, a combined fluidic and magnetic force finite element model simulation was created using the software COMSOL Multiphysics and compared with separation experiments. Finally, as optimal lattice orientation of the separation matrix, a transversal rhombohedral arrangement was defined. The high suitability of the new filter matrix has been verified by separation experiments.}, language = {en} } @misc{TippkoetterDuweRaisetal.2014, author = {Tippk{\"o}tter, Nils and Duwe, Anna and Rais, Dominik and Zibek, Susanne and Zorn, H.}, title = {Optimierung und Scale-up der enzymatischen Hydrolyse inkl. Ligninabbau}, series = {Chemie Ingenieur Technik}, volume = {86}, journal = {Chemie Ingenieur Technik}, number = {9}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0009-286X}, doi = {10.1002/cite.201450287}, pages = {1515}, year = {2014}, abstract = {Prim{\"a}re Ziele der Hydrolyse pflanzlicher nachwachsender Rohstoffe sind m{\"o}glichst hohe Zuckerkonzentrationen f{\"u}r nachfolgende Fermentationen und eine Maximierung der Produktivit{\"a}t. Zur Optimierung dieser Prozesse wird Organosolv-aufgeschlossene Buchenholz-Cellulose verwendet. Die Hydrolyse des Faserstoffes erfolgt mithilfe von Novozymes CTec2-Enzymen. Die Hydrolysen konnten durch neue R{\"u}hrerelemente auf einen Maßstab von 1000 L {\"u}bertragen werden. Dabei konnten maximale Ausbeuten (g Glucose g -1 Glucose im Faserstoff) bis 81 g g - 1 und Konzentrationen von 152 g L -1 erreicht werden. Zurzeit k{\"o}nnen unter Einsatz eines Feststoffreaktors Cellulosefasern in einer Konzentration bis 400 g L -1 enzymatisch hydrolysiert werden. Die cellulolytischen Enzyme stoßen bei hohen Feststoffkonzentrationen an ihre Grenzen. Mit steigendem Feststoffgehalt nimmt die Hydrolyseausbeute ab. Ein Ansatz zur Steigerung der Effizienz ist der Einsatz ligninolytischer Enzyme, die Ligninreste an der Organosolv-Cellulose aufschließen k{\"o}nnen. Eine solche Verbesserung der Zug{\"a}nglichkeit f{\"u}r cellulolytische Enzyme an ihr Substrat wurde durch Kultur{\"u}berst{\"a}nde verschiedener ligninolytischer Pilze erreicht. Mit Kultur{\"u}berst{\"a}nden von Stereum sp. sind Steigerungen der Glucoseausbeuten um bis zu 30 \% m{\"o}glich.}, language = {de} } @article{RatkeMilowLisinskietal.2014, author = {Ratke, Lorenz and Milow, Barbara and Lisinski, Susanne and Hoepfner, Sandra}, title = {On an effect of fine ceramic particles on the structure of aerogels}, series = {Microgravity science and technology}, volume = {26}, journal = {Microgravity science and technology}, publisher = {Springer Nature}, address = {Heidelberg}, issn = {0938-0108 ; 1875-0494}, doi = {10.1007/s12217-014-9380-2}, pages = {103 -- 110}, year = {2014}, language = {en} } @misc{TippkoetterMoehring2014, author = {Tippk{\"o}tter, Nils and M{\"o}hring, S.}, title = {Nutzung von F{\"a}ulepilzen f{\"u}r die selektive Gewinnung von Cellulose und Lignin aus nicht vorbehandelter lignocellulosehaltiger Biomasse}, series = {Chemie Ingenieur Technik}, volume = {86}, journal = {Chemie Ingenieur Technik}, number = {9}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0009-286X}, doi = {10.1002/cite.201450353}, pages = {1385}, year = {2014}, abstract = {Einige Arten der Braun- und Weißf{\"a}ulepilze sind in der Lage, selektiv entweder Lignin oder Cellulose im Holz abzubauen. Diese Pilze k{\"o}nnen f{\"u}r eine energiesparende Vorbehandlung lignocellulosehaltiger Biomasse f{\"u}r Bioraffinerien genutzt werden, ohne auf technisch aufw{\"a}ndige Aufschlussapparate zur{\"u}ckgreifen zu m{\"u}ssen. Weißf{\"a}ulepilze bauen bevorzugt Lignin ab, wodurch die verbleibende Cellulose leichter f{\"u}r enzymatische Hydrolysen in das Monosaccharid Glucose zug{\"a}nglich wird. Braunf{\"a}ulepilze bauen dagegen Cellulose und Hemicellulose ab. Die Auswirkungen der Behandlung von Weizenstroh mit verschiedenen Pilzarten werden zurzeit untersucht. Dabei werden die Ver{\"a}nderung der enzymatischen Hydrolysierbarkeit des Substrats sowie die gebildeten Ligninderivate bestimmt. Detaillierte Betrachtungen der Biomassever{\"a}nderung werden mithilfe spezifischer F{\"a}rbemethoden durchgef{\"u}hrt, durch die morphologische Ver{\"a}nderungen der Pflanzengewebe in der 3D-Lichtmikroskopie dargestellt werden k{\"o}nnen.}, language = {de} } @inproceedings{OertelBung2014, author = {Oertel, Mario and Bung, Daniel Bernhard}, title = {Numerische Str{\"o}mungssimulation von Fließgew{\"a}ssern - Wo stehen wir und was werden wir im Laufe der n{\"a}chsten Jahre erreichen?}, series = {Simulationsverfahren und Modelle f{\"u}r Wasserbau und Wasserwirtschaft = Simulation techniques and models for hydraulic engineering and water management / 37. Dresdner Wasserbaukolloquium 2014, 13. - 14. M{\"a}rz 2014. (Dresdner wasserbauliche Mitteilungen ; H. 50)}, booktitle = {Simulationsverfahren und Modelle f{\"u}r Wasserbau und Wasserwirtschaft = Simulation techniques and models for hydraulic engineering and water management / 37. Dresdner Wasserbaukolloquium 2014, 13. - 14. M{\"a}rz 2014. (Dresdner wasserbauliche Mitteilungen ; H. 50)}, organization = {Dresdner Wasserbaukolloquium <37, 2014>}, isbn = {978-3-86780-349-6}, pages = {19 -- 30}, year = {2014}, language = {de} }