@article{DallasSalphatiGomezZepedaetal.2016,
  author    = {Dallas, Shannon and Salphati, Laurent and Gomez-Zepeda, David and Wanek, Thomas and Chen, Liangfu and Chu, Xiaoyan and Kunta, Jeevan and Mezler, Mario and Menet, Marie-Claude and Chasseigneaux, Stephanie and Decl{\`e}ves, Xavier and Langer, Oliver and Pierre, Esaie and DiLoreto, Karen and Hoft, Carolin and Laplanche, Loic and Pang, Jodie and Pereira, Tony and Andonian, Clara and Simic, Damir and Rode, Anja and Yabut, Jocelyn and Zhang, Xiaolin and Scheer, Nico},
  title     = {Generation and Characterization of a Breast Cancer Resistance Protein Humanized Mouse Model},
  series = {Molecular Pharmacology},
  volume    = {89},
  journal   = {Molecular Pharmacology},
  number    = {5},
  publisher = {ASPET},
  address   = {Bethesda, Md.},
  issn      = {1521-0111},
  doi       = {10.1124/mol.115.102079},
  pages     = {492 -- 504},
  year      = {2016},
  abstract  = {Breast cancer resistance protein (BCRP) is expressed in various tissues, such as the gut, liver, kidney and blood brain barrier (BBB), where it mediates the unidirectional transport of substrates to the apical/luminal side of polarized cells. Thereby BCRP acts as an efflux pump, mediating the elimination or restricting the entry of endogenous compounds or xenobiotics into tissues and it plays important roles in drug disposition, efficacy and safety. Bcrp knockout mice (Bcrp-/-) have been used widely to study the role of this transporter in limiting intestinal absorption and brain penetration of substrate compounds. Here we describe the first generation and characterization of a mouse line humanized for BCRP (hBCRP), in which the mouse coding sequence from the start to stop codon was replaced with the corresponding human genomic region, such that the human transporter is expressed under control of the murine Bcrp promoter. We demonstrate robust human and loss of mouse BCRP/Bcrp mRNA and protein expression in the hBCRP mice and the absence of major compensatory changes in the expression of other genes involved in drug metabolism and disposition. Pharmacokinetic and brain distribution studies with several BCRP probe substrates confirmed the functional activity of the human transporter in these mice. Furthermore, we provide practical examples for the use of hBCRP mice to study drug-drug interactions (DDIs). The hBCRP mouse is a promising model to study the in vivo role of human BCRP in limiting absorption and BBB penetration of substrate compounds and to investigate clinically relevant DDIs involving BCRP.},
  language  = {en}
}
@misc{DuweSchlegelTippkoetteretal.2014,
  author    = {Duwe, A. and Schlegel, C. and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Sequentielle Extraktion von Cellulose zur effizienten Nutzung der Stoffstr{\"o}me in der Holzbioraffinerie},
  series = {Chemie Ingenieur Technik},
  volume    = {86},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0009-286X},
  doi       = {10.1002/cite.201450308},
  pages     = {1400},
  year      = {2014},
  abstract  = {In der Reihe der nachwachsenden Rohstoffe besitzt Holz als erneuerbare und umweltfreundliche Ressource ein großes Potenzial. {\"U}ber 11 Mio. ha Holz, das laut der Fachagentur f{\"u}r nachwachsende Rohstoffe (FNR) auch f{\"u}r industrielle Zwecke genutzt werden kann, wuchsen im Jahr 2013 allein auf bundesdeutscher Fl{\"a}che. 56,8 Mio. m³ j{\"a}hrlicher Holzeinschlag in den letzten zehn Jahren wurde zu knapp der H{\"a}lfte stofflich und der Rest energetisch verwertet. Im Rahmen dieser Arbeit konnte auf der Basis vom Holz der Buche, die nach Fichte und Kiefer die dritth{\"a}ufigste Baumart in Deutschland ist und 15\% der deutschen Waldfl{\"a}che ausmacht, die Fraktionierung der polymeren Hauptbestandteile mit niedrigem energetischen Einsatz erreicht werden. Hierbei werden in einem nachgeschalteten Extraktionsprozess die beiden Komponenten Hemicellulose und Lignin in fl{\"u}ssiger Form von der finalen festen Cellulosefraktion abgetrennt. Die Extraktion der Hemicellulose erfolgt durch eine Liquid Hot Water (LHW)-Behandlung. Untersucht wird der katalytische Zusatz anorganischer S{\"a}uren wie H₃PO₄ und H₂SO₄. Im Hinblick auf die weitere Verwertung von Lignin zu aromatischen Synthesebausteinen kommt die Organosolv-Extraktion mit einem Ethanol/Wasser-Gemisch zum Einsatz. Von Vorteil ist die weitere Verwendung beider Stoffstr{\"o}me ohne F{\"a}llungsschritt und nachteiliger Verd{\"u}nnung der Hemicellulose.},
  language  = {en}
}
@incollection{HahnKellyMuffleretal.2011,
  author    = {Hahn, Thomas and Kelly, Svenja and Muffler, Kai and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Extraction of lignocellulose and algae for the production of bulk and fine chemicals},
  series = {Industrial scale natural products extraction},
  booktitle = {Industrial scale natural products extraction},
  editor    = {Hans-J{\"o}rg, Bart and Pilz, Stephan},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  isbn      = {978-3-527-32504-7 (Print)},
  doi       = {10.1002/9783527635122},
  pages     = {221 -- 245},
  year      = {2011},
  language  = {en}
}
@misc{AlKaidyTippkoetterKaiseretal.2014,
  author    = {Al-Kaidy, H. and Tippk{\"o}tter, Nils and Kaiser, P. and Wollny, S. and Ulber, Roland},
  title     = {Aufreinigung von Cytochrom P450BMP mittels magnetischer Partikel und die enzymatische Synthese von 9, 10-Dihydroxystearins{\"a}ure},
  series = {Chemie Ingenieur Technik},
  volume    = {86},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {0009-286X},
  doi       = {10.1002/cite.201450420},
  pages     = {1420},
  year      = {2014},
  abstract  = {Cytochrom P450 sind H{\"a}m-Proteine, die zur Enzymklasse der Oxidoreduktasen (EC 1.14.xy) geh{\"o}ren. Eine wichtige Reaktion ist die Hydroxylierung nichtaktivierter C-H-Bindungen, die in technischen Systemen von großem Interesse ist. Durch die Verwendung von M-IDA-2-Partikeln ist eine direkte Aufreinigung mit gleichzeitiger Immobilisierung und die Applikation der Enzyme aus dem Zelllysat m{\"o}glich. Damit ist das Verfahren mehr als f{\"u}nf Stunden schneller als die konventionelle Chromatographie und mehr als 80 \% der Aufreinigungszeit wird gespart. Mit dem isolierten nativen Enzym konnte die Plattformchemikalie 9,10-Dihydroxystearins{\"a}ure aus {\"O}ls{\"a}ure hergestellt werden. Unter anderem f{\"u}r die Kunststoffindustrie k{\"o}nnen aus diesem Produkt wichtige Monomere wie z. B. Azelains{\"a}ure hergestellt werden. Die Bildung des Produkts erfolgt in einem zweiphasigen Reaktionssystem an der Grenzfl{\"a}che zwischen dem {\"O}l und der w{\"a}ssrigen Phase als Feststoff. Um das immobilisierte Enzym aktiv in die obere Phase zu transportieren, wurde eine neue magnetische Mischvorrichtung entwickelt. Das Reaktionsprodukt wurde mit NMR, GC-MS und HPLC-MS analysiert und mit einem chemisch synthetisierten Standard von 9,10-Dihydroxystearins{\"a}ure verglichen. Derzeit werden Studien des immobilisierten H{\"a}ms des Enzyms durchgef{\"u}hrt.},
  language  = {de}
}
@misc{HeringUlberTippkoetter2016,
  author    = {Hering, T. and Ulber, Roland and Tippk{\"o}tter, Nils},
  title     = {Antimikrobielle Oberfl{\"a}chenmodifikation durch Mikropartikel},
  series = {Chemie Ingenieur Technik},
  volume    = {88},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  doi       = {10.1002/cite.201650084},
  pages     = {1302},
  year      = {2016},
  abstract  = {Die Ausbildung von Biofilmen in technischen Anlagen, wie z. B. K{\"u}hlkreisl{\"a}ufen, Wasseraufbereitungssystemen und Bioreaktoren, f{\"u}hren zu Materialsch{\"a}den (Biofouling) und stark erh{\"o}htem Energieaufwand. Im Rahmen der aktuellen Forschungsarbeiten erfolgen aktive sowie passive Bio-Modifikationen auf funktionalisierten magnetischen Mikropartikelober-fl{\"a}chen. Um die verschiedenen funktionalisierten magnetischen Mikropartikel zu analysieren und ihre antimikrobielle Wirkung zu testen, wird der Einsatz einer 3D-gedruckten, magnetischen Plattform f{\"u}r ein Fluoreszenz-basiertes Screening-System untersucht. F{\"u}r den Oberfl{\"a}chenschutz wurden verschiedene, antimikrobiell funktionalisierte Partikelkombinationen mit dem Mikroorganismus Escherichia coli GFPmut2 in Bezug auf aktiven Oberfl{\"a}chenschutz verglichen. Um die antimikrobielle Oberfl{\"a}cheneffekte von synergistischen Kombinationen unterschiedlich funktionalisierter Partikel zu bestimmen, werden Oberfl{\"a}chen einem Magnetfeld ausgesetzt, das die Mikropartikel als definierte Schicht auf ihnen zur{\"u}ck h{\"a}lt. Diese modifizierten Oberfl{\"a}chen k{\"o}nnen sowohl durch Fluoreszenzspektroskopie als auch -mikroskopie analysiert werden.},
  language  = {de}
}
@article{Berndt1979,
  author    = {Berndt, Heinz},
  title     = {Synthese der Sequenz 71—86 des Humanproinsulins, I : Synthese der Sequenz 71—86 als monomeres cyclisches Biscystinpeptidderivat und als Tetra-S-tritylderivat},
  series = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie},
  volume    = {360},
  journal   = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie},
  number    = {1},
  issn      = {1437-4315},
  doi       = {10.1515/bchm2.1979.360.1.747},
  pages     = {747 -- 760},
  year      = {1979},
  language  = {de}
}
@article{TeumerCapitainRossJonesetal.2018,
  author    = {Teumer, T. and Capitain, C. and Ross-Jones, J. and Tippk{\"o}tter, Nils and R{\"a}dle, M. and Methner, F.-J.},
  title     = {In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor},
  series = {BrewingScience},
  volume    = {71},
  journal   = {BrewingScience},
  number    = {5/6},
  publisher = {Fachverlag Hans Carl},
  address   = {N{\"u}rnberg},
  issn      = {1613-2041},
  pages     = {49 -- 55},
  year      = {2018},
  abstract  = {In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented.},
  language  = {en}
}
@techreport{TippkoetterWagner2019,
  author    = {Tippk{\"o}tter, Nils and Wagner, Sebastian},
  title     = {Biomimetischer Klebstoff aus ligninhaltigen Pflanzenresten (Teilvorhaben 1 und 2) : Schlussbericht zum Vorhaben : Laufzeit: 01.01.2016 bis 31.03.2019},
  publisher = {FH Aachen},
  address   = {J{\"u}lich},
  doi       = {10.2314/KXP:169732777X},
  pages     = {109 S.},
  year      = {2019},
  language  = {de}
}
@article{RoeschKratzHeringetal.2016,
  author    = {R{\"o}sch, C. and Kratz, F. and Hering, T. and Trautmann, S. and Umanskaya, N. and Tippk{\"o}tter, Nils and M{\"u}ller-Renno, C.M. and Ulber, Roland and Hannig, M. and Ziegler, C.},
  title     = {Albumin-lysozyme interactions: cooperative adsorption on titanium and enzymatic activity},
  series = {Colloids and Surfaces B: Biointerfaces},
  volume    = {149},
  journal   = {Colloids and Surfaces B: Biointerfaces},
  number    = {1},
  publisher = {Elsevier},
  address   = {Amsterdam},
  doi       = {10.1016/j.colsurfb.2016.09.048},
  pages     = {115 -- 121},
  year      = {2016},
  abstract  = {The interplay of albumin (BSA) and lysozyme (LYZ) adsorbed simultaneously on titanium was analyzed by gel electrophoresis and BCA assay. It was found that BSA and lysozyme adsorb cooperatively. Additionally, the isoelectric point of the respective protein influences the adsorption. Also, the enzymatic activity of lysozyme and amylase (AMY) in mixtures with BSA was considered with respect to a possible influence of protein-protein interaction on enzyme activity. Indeed, an increase of lysozyme activity in the presence of BSA could be observed. In contrast, BSA does not influence the activity of amylase.},
  language  = {en}
}
@article{MolinnusBaeckerSiegertetal.2015,
  author    = {Molinnus, Denise and B{\"a}cker, Matthias and Siegert, Petra and Willenberg, H. and Poghossian, Arshak and Keusgen, M. and Sch{\"o}ning, Michael Josef},
  title     = {Detection of Adrenaline Based on Substrate Recycling Amplification},
  series = {Procedia Engineering},
  volume    = {120},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2015.08.708},
  pages     = {540 -- 543},
  year      = {2015},
  abstract  = {An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.},
  language  = {en}
}
@article{TippkoetterWollnyKampeisetal.2011,
  author    = {Tippk{\"o}tter, Nils and Wollny, S. and Kampeis, P. and Oster, J. and Schneider, H. and Ulber, Roland},
  title     = {Magnetseparation von Proteinen : Separation von Zielmolek{\"u}len durch hochselektive Aptamere},
  series = {GIT Labor-Fachzeitschrift},
  volume    = {55},
  journal   = {GIT Labor-Fachzeitschrift},
  number    = {10},
  publisher = {Wiley},
  address   = {Weinheim},
  pages     = {666},
  year      = {2011},
  abstract  = {Durch die Kombination von Oligonukleotid-Liganden (Aptameren) hoher Bindungsaffinit{\"a}ten mit hochselektiv abtrennbaren magnetisierbaren Mikropartikeln wird eine einstufige Separation von Zielmolek{\"u}len aus mikrobiologischen Produktionsans{\"a}tzen m{\"o}glich. Die Aptamere werden hierf{\"u}r reversibel auf den Partikeloberfl{\"a}chen gebunden und f{\"u}r die spezifische Isolierung von Bioprodukten eingesetzt. Die Abtrennung der beladenen Partikel erfolgt durch einen neuen Rotor-Stator-Separator mit Hochgradient-Magnetfeld.},
  language  = {de}
}
@incollection{WagemannTippkoetter2018,
  author    = {Wagemann, Kurt and Tippk{\"o}tter, Nils},
  title     = {Biorefineries: a short introduction},
  series = {Biorefineries},
  booktitle = {Biorefineries},
  publisher = {Springer},
  address   = {Cham},
  isbn      = {978-3-319-97117-9},
  doi       = {10.1007/10_2017_4},
  pages     = {1 -- 11},
  year      = {2018},
  abstract  = {The terms bioeconomy and biorefineries are used for a variety of processes and developments. This short introduction is intended to provide a delimitation and clarification of the terminology as well as a classification of current biorefinery concepts. The basic process diagrams of the most important biorefinery types are shown.},
  language  = {en}
}
@article{WulfhorstDuweMerseburgetal.2016,
  author    = {Wulfhorst, Helene and Duwe, Anna-Maria and Merseburg, Johannes and Tippk{\"o}tter, Nils},
  title     = {Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis},
  series = {Tetrahedron},
  volume    = {72},
  journal   = {Tetrahedron},
  number    = {46},
  publisher = {Elsevier},
  address   = {Amsterdam},
  doi       = {10.1016/j.tet.2016.04.029},
  pages     = {7329 -- 7334},
  year      = {2016},
  abstract  = {The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation.},
  language  = {en}
}
@techreport{Tippkoetter2018,
  author    = {Tippk{\"o}tter, Nils},
  title     = {Lokale Vorbehandlung nachwachsender Rohstoffe f{\"u}r Bioraffinerien (BioSats) : Schlussbericht zum Vorhaben : Laufzeit: 01.03.2012 bis 30.04.2017},
  organization    = {Technische Universit{\"a}t Kaiserslautern},
  doi       = {10.2314/GBV:1024204243},
  pages     = {191 Seiten},
  year      = {2018},
  language  = {de}
}
@article{WackwitzBongaertsGoodmanetal.1999,
  author    = {Wackwitz, B. and Bongaerts, Johannes and Goodman, S. D. and Unden, Gottfried},
  title     = {Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance},
  series = {Molecular and general genetics : MGG},
  volume    = {Vol. 262},
  journal   = {Molecular and general genetics : MGG},
  number    = {Iss. 4 - 5},
  issn      = {1617-4623 (E-Journal); 1617-4615 (Print)},
  pages     = {876 -- 883},
  year      = {1999},
  language  = {en}
}
@article{TippkoetterRoikaewUlber2008,
  author    = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, Roland},
  title     = {Nitrate removal from whey concentrate with biotechnological regeneration of the waste water},
  series = {European dairy magazine : EDM},
  journal   = {European dairy magazine : EDM},
  number    = {1},
  isbn      = {0936-6318},
  pages     = {30 -- 32},
  year      = {2008},
  language  = {en}
}
@article{UndenBeckerBongaertsetal.1995,
  author    = {Unden, G. and Becker, S. and Bongaerts, Johannes and Holighaus, G. and Schirawski, J. and Six, S.},
  title     = {O2-sensing and O2-dependent gene regulation in facultatively anaerobic bacteria},
  series = {Archives of microbiology},
  volume    = {Vol. 164},
  journal   = {Archives of microbiology},
  number    = {Iss. 2},
  issn      = {1432-072X (E-Journal); 0003-9276 (Print); 0302-8933 (Print)},
  pages     = {81 -- 90},
  year      = {1995},
  language  = {en}
}
@inproceedings{TippkoetterStueckmannWinkelmannetal.2007,
  author    = {Tippk{\"o}tter, Nils and St{\"u}ckmann, H. and Winkelmann, G. and Noack, U. and Beutel, S. and Scheper, T. and Ulber, Roland},
  title     = {Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR},
  series = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme},
  booktitle = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme},
  publisher = {Dechema},
  address   = {Frankfurt am Main},
  pages     = {126},
  year      = {2007},
  language  = {en}
}
@misc{AlKaidyTippkoetterUlber2016,
  author    = {Al-Kaidy, Huschyar and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Vorrichtung und Verfahren zur Bestimmung des Kontaktwinkels eines Fl{\"u}ssigk{\"o}rpers mit einer Festk{\"o}rperoberfl{\"a}che},
  year      = {2016},
  abstract  = {Die vorliegende Erfindung betrifft eine Vorrichtung und ein Verfahren zur Bestimmung des Kontaktwinkels eines fl{\"u}ssigen oder mit Fl{\"u}ssigkeit gef{\"u}llten K{\"o}rpers. Dieser besteht aus einem Tr{\"a}ger (1) und einer damit verbundenen, in einem Winkelbereich von mehr als 0 ° bis maximal 90 ° neigbaren Ebene (8) mit einer darin ausgebildeten Abrollbahn (9) f{\"u}r den fl{\"u}ssigen oder mit Fl{\"u}ssigkeit gef{\"u}llten K{\"o}rper. An der Ebene (8) sind mehrere Sensoren (11,12) zur Erfassung der Rolldauer des K{\"o}rpers entlang der Rollstrecke angeordnet. Erfindungsgem{\"a}ß ist vorgesehen, dass die Einstellung des Neigungswinkels der Ebene (8) {\"u}ber ein Winkelmessger{\"a}t (10) erfolgt, wodurch ein Abrollwinkel erfassbar ist, bei dem der K{\"o}rper in Bewegung ger{\"a}t. Aus der Rolldauer, der Rollstrecke und dem Abrollwinkel wird der Kontaktwinkel des K{\"o}rpers ermittelt.},
  language  = {de}
}
@article{TippkoetterDeterdingUlber2008,
  author    = {Tippk{\"o}tter, Nils and Deterding, A. and Ulber, Roland},
  title     = {Determination of acetic acid in fermentation broth by gas-diffusion technique},
  series = {Engineering in Life Sciences},
  volume    = {8},
  journal   = {Engineering in Life Sciences},
  number    = {1, Special Issue: Technical Systems for the Use in Life Sciences},
  doi       = {10.1002/elsc.200820227},
  pages     = {62 -- 67},
  year      = {2008},
  abstract  = {Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5-5.0 g/L acetic acid with a relative standard deviation of <5 \% was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.},
  language  = {en}
}