@article{RoehlenPilasSchoeningetal.2017, author = {R{\"o}hlen, Desiree and Pilas, Johanna and Sch{\"o}ning, Michael Josef and Selmer, Thorsten}, title = {Development of an amperometric biosensor platform for the combined determination of l-Malic, Fumaric, and l-Aspartic acid}, series = {Applied Biochemistry and Biotechnology}, volume = {183}, journal = {Applied Biochemistry and Biotechnology}, publisher = {Springer}, address = {Berlin}, issn = {1559-0291}, doi = {10.1007/s12010-017-2578-1}, pages = {566 -- 581}, year = {2017}, abstract = {Three amperometric biosensors have been developed for the detection of L-malic acid, fumaric acid, and L -aspartic acid, all based on the combination of a malate-specific dehydrogenase (MDH, EC 1.1.1.37) and diaphorase (DIA, EC 1.8.1.4). The stepwise expansion of the malate platform with the enzymes fumarate hydratase (FH, EC 4.2.1.2) and aspartate ammonia-lyase (ASPA, EC 4.3.1.1) resulted in multi-enzyme reaction cascades and, thus, augmentation of the substrate spectrum of the sensors. Electrochemical measurements were carried out in presence of the cofactor β-nicotinamide adenine dinucleotide (NAD+) and the redox mediator hexacyanoferrate (III) (HCFIII). The amperometric detection is mediated by oxidation of hexacyanoferrate (II) (HCFII) at an applied potential of + 0.3 V vs. Ag/AgCl. For each biosensor, optimum working conditions were defined by adjustment of cofactor concentrations, buffer pH, and immobilization procedure. Under these improved conditions, amperometric responses were linear up to 3.0 mM for L-malate and fumarate, respectively, with a corresponding sensitivity of 0.7 μA mM-1 (L-malate biosensor) and 0.4 μA mM-1 (fumarate biosensor). The L-aspartate detection system displayed a linear range of 1.0-10.0 mM with a sensitivity of 0.09 μA mM-1. The sensor characteristics suggest that the developed platform provides a promising method for the detection and differentiation of the three substrates.}, language = {en} } @article{BiselliVanderPolJokschetal.1992, author = {Biselli, Manfred and Van der Pol, Jens J. and Joksch, B. and Eberhardt, R.}, title = {Development of an enzymatic multichannel flow injection analysis system for monitoring mammalian cell fermentations / Van der Pol, J.J.; Joksch, B.; Eberhardt, R.; Biselli, M.; Wandrey, C., Tramper, J.}, series = {Biosensors : fundamentals, technologies and applications ; contributions to the BMFT status seminar with international participation May, 12 to 14, 1991, Internationales Bildungs-Centrum Bogensee/Brandenburg, Germany / org. by ZIM-Zentralinstitut f{\"u}r Molekularbiologie, Berlin-Buch and GBF, Braunschweig. Ed. by F. Scheller}, journal = {Biosensors : fundamentals, technologies and applications ; contributions to the BMFT status seminar with international participation May, 12 to 14, 1991, Internationales Bildungs-Centrum Bogensee/Brandenburg, Germany / org. by ZIM-Zentralinstitut f{\"u}r Molekularbiologie, Berlin-Buch and GBF, Braunschweig. Ed. by F. Scheller}, publisher = {VCH}, address = {Weinheim}, isbn = {3527284370}, pages = {349 -- 352}, year = {1992}, language = {en} } @inproceedings{TippkoetterMoehringMaureretal.2013, author = {Tippk{\"o}tter, Nils and M{\"o}hring, S. and Maurer, S. and Roth, J.}, title = {Dezentrale Vorbehandlung und Verarbeitung pflanzlicher Reststoffe f{\"u}r Bioraffinerien}, series = {Kurzfassungen der Vortr{\"a}ge nach Sessions : Fr{\"u}hjahrstagung der Biotechnologen 2013, 4. - 5. M{\"a}rz 2013, Dechema-Haus, Frankfurt am Main}, booktitle = {Kurzfassungen der Vortr{\"a}ge nach Sessions : Fr{\"u}hjahrstagung der Biotechnologen 2013, 4. - 5. M{\"a}rz 2013, Dechema-Haus, Frankfurt am Main}, address = {Frankfurt am Main}, pages = {5}, year = {2013}, language = {de} } @article{BaumannJahnke1986, author = {Baumann, Marcus and Jahnke, J.}, title = {Die marine Planktonalge Phaeocystis globosa / Jahnke, J. ; Baumann, M.}, series = {Mikrokosmos. 75 (1986), H. 12}, journal = {Mikrokosmos. 75 (1986), H. 12}, isbn = {0026-3680}, pages = {357 -- 359}, year = {1986}, language = {de} } @misc{KowollikSchnitzlerBisellietal.2010, author = {Kowollik, Silvia and Schnitzler, Thomas and Biselli, Manfred and Krueger, R. and Zang, Werner and Peuscher, A. and Schillberg, S. and Fischer, R.}, title = {Die Rolle des Respirationsquotienten in der Zellkulturfermentation}, series = {Chemie Ingenieur Technik}, volume = {82}, journal = {Chemie Ingenieur Technik}, number = {9}, publisher = {Wiley-VCH}, address = {Weinheim}, isbn = {Chemie Ingenieur Tec}, doi = {10.1002/cite.201050393}, pages = {1505 -- 1506}, year = {2010}, abstract = {In der biopharmazeutischen Industrie werden rekombinante Proteine und monoklonale Antik{\"o}rper in Zellkulturfermentationen produziert, da nur humane oder tierische Zelllinien {\"u}ber die F{\"a}higkeit der Glykosylierung verf{\"u}gen. Um hohe Produktausbeuten in ausgezeichneter Qualit{\"a}t zu erzielen, ist eine funktionst{\"u}chtige Prozesskontrolle unerl{\"a}sslich. Hierzu wurde in Kooperation mit der Firma Hitec Zang GmbH die HiSense� Pr{\"a}zisionsabgasanalytik entwickelt, die auf Basis der vollautomatischen Ermittlung des Respirationsquotienten (RQ; Verh{\"a}ltnis vonKohlendioxidbildungsrate (CER) zu Sauerstoffaufnahmerate (OTR)) einen Fermentationsprozess nicht-invasiv {\"u}berwacht. Der RQ kann in Hybridoma- und CHO-Zellen (s. Abb.) in sowohl serumhaltigen als auch serumfreien Medien erfolgreich ermittelt werden. Hier spiegeln die CER und die OTR das Wachstumsverhalten der kultivierten CHO-Zellen wider. Der RQ nimmt dabei Werte zwischen 0,9 und 1,2 an. Dies l{\"a}sst auf verschiedene Stoffwechselaktivit{\"a}ten schließen. Da die momentane industrielle Prozesskontrolle auf gemessenen Sauerstoffaufnahmeraten oder entsprechende Offline-Analytiken der Metaboliten basieren, soll durch die vollautomatische RQ-Ermittlung ein neues Verfahren zur Fermentations{\"u}berwachung etabliert werden. Bisher war diese, in bakteriellen Kultivierungen standardisierte Methode, aufgrund der schwierigen CER-Berechnung bei Zellkulturen keine ad{\"a}quate Alternative.}, language = {de} } @article{GrosshauserPettrak2023, author = {Großhauser, Christian and Pettrak, J{\"u}rgen}, title = {Die Rolle des Wasserstoffs in der Abwasserbehandlung}, series = {Wasser und Abfall}, journal = {Wasser und Abfall}, number = {7/8}, publisher = {Springer Fachmedien}, address = {Wiesbaden}, issn = {1436-9095}, doi = {10.1007/s35152-023-1444-4}, year = {2023}, abstract = {Die Bereitstellung von nachhaltig erzeugtem Wasserstoff als Energietr{\"a}ger und Rohstoff ist eine wichtige Schl{\"u}sseltechnologie sowohl als Ersatz f{\"u}r fossile Energietr{\"a}ger, aber auch als Produkt im Zusammenhang mit Kreislaufprozessen. In der Abwasserbehandlung bestehen verschiedene M{\"o}glichkeiten Wasserstoff herzustellen. Mehrere Wege, m{\"o}gliche Synergien, aber auch deren Nachteile werden vorgestellt.}, language = {de} } @article{BiselliNoll1998, author = {Biselli, Manfred and Noll, T.}, title = {Dielectric spectroscopy in the cultivation of suspended and immobilized hybridoma cells / Noll, T.; Biselli, M.}, series = {Journal of Biotechnology. 63 (1998), H. 3}, journal = {Journal of Biotechnology. 63 (1998), H. 3}, isbn = {0168-1656}, pages = {187 -- 198}, year = {1998}, language = {en} } @article{CapitainRossJonesMoehringetal.2020, author = {Capitain, Charlotte and Ross-Jones, Jesse and M{\"o}hring, Sophie and Tippk{\"o}tter, Nils}, title = {Differential scanning calorimetry for quantification of polymer biodegradability in compost}, series = {International Biodeterioration \& Biodegradation}, volume = {149}, journal = {International Biodeterioration \& Biodegradation}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0964-8305}, doi = {10.1016/j.ibiod.2020.104914}, pages = {In Press, Article number 104914}, year = {2020}, abstract = {The objective of this study is the establishment of a differential scanning calorimetry (DSC) based method for online analysis of the biodegradation of polymers in complex environments. Structural changes during biodegradation, such as an increase in brittleness or crystallinity, can be detected by carefully observing characteristic changes in DSC profiles. Until now, DSC profiles have not been used to draw quantitative conclusions about biodegradation. A new method is presented for quantifying the biodegradation using DSC data, whereby the results were validated using two reference methods. The proposed method is applied to evaluate the biodegradation of three polymeric biomaterials: polyhydroxybutyrate (PHB), cellulose acetate (CA) and Organosolv lignin. The method is suitable for the precise quantification of the biodegradability of PHB. For CA and lignin, conclusions regarding their biodegradation can be drawn with lower resolutions. The proposed method is also able to quantify the biodegradation of blends or composite materials, which differentiates it from commonly used degradation detection methods.}, language = {en} } @article{JahnkeBaumann1987, author = {Jahnke, J. and Baumann, Marcus}, title = {Differentiation between Phaeocystis pouchetii (Har.) Lagerheim and Phaeocystis globosa Scherffel}, series = {Hydrobiological bulletin}, volume = {Vol. 21}, journal = {Hydrobiological bulletin}, number = {Iss. 2}, issn = {0165-1404 (Print); 1573-5125 (E-Journal)}, pages = {141 -- 147}, year = {1987}, language = {en} } @article{BiselliSchroederHerfurthetal.1997, author = {Biselli, Manfred and Schr{\"o}der, B. and Herfurth, C. and Meißner, P.}, title = {Differentiation of bone marrow cells immobilized on microcarriers in a fluidized bed reactor / Schr{\"o}der, B. ; Herfurth, C. ; Meißner, P. ; Biselli, M. ; Link, H. ; Wandrey, C.}, series = {Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo}, journal = {Animal cell technology : from vaccines to genetic medicine ; [Vilamoura, Portugal, May 1996] / ed. by Manuel J. T. Carrondo}, publisher = {Kluwer}, address = {Dordrecht}, isbn = {0-7923-4321-2}, pages = {589 -- 593}, year = {1997}, language = {en} } @article{KotterHeeringRiekert1982, author = {Kotter, Michael and Heering, J. and Riekert, L.}, title = {Diffusion and catalytic reaction in zeolite ZSM-5 / J. Heering ; M. Kotter ; L. Riekert}, series = {Chemical engineering science . 37 (1982), H. 4}, journal = {Chemical engineering science . 37 (1982), H. 4}, isbn = {0009-2509}, pages = {581 -- 584}, year = {1982}, language = {en} } @article{PrielmeierSpeedyVardagetal.1989, author = {Prielmeier, Franz and Speedy, R. J. and Vardag, T. and Lang, E. W.}, title = {Diffusion in simple fluids / R. J. Speedy; F. X. Prielmeier; T. Vardag; E. W. Lang; H.-D. L{\"u}demann}, series = {Molecular Physics. 66 (1989), H. 3}, journal = {Molecular Physics. 66 (1989), H. 3}, isbn = {0026-8976}, pages = {577 -- 590}, year = {1989}, language = {en} } @article{PrielmeierLangSpeedyetal.1987, author = {Prielmeier, Franz and Lang, E. W. and Speedy, R. J. and L{\"u}demann, H.-D.}, title = {Diffusion in supercooled water to 300 MPa}, series = {Physical Review Letters. 59 (1987), H. 10}, journal = {Physical Review Letters. 59 (1987), H. 10}, isbn = {0031-9007}, pages = {1128 -- 1131}, year = {1987}, language = {en} } @article{JerominWelsch1995, author = {Jeromin, G{\"u}nter Erich and Welsch, Volker}, title = {Diketene a New Esterification Reagent in the Enzyme-Aided Synthesis of Chiral Alcohols and Chiral Acetoacetic Acid Esters}, series = {Tetrahedron Letters . 36 (1995), H. 37}, journal = {Tetrahedron Letters . 36 (1995), H. 37}, isbn = {0040-4039}, pages = {6663 -- 6664}, year = {1995}, language = {en} } @article{BaumannLaraHubbertenetal.1997, author = {Baumann, Marcus and Lara, R.J. and Hubberten, D.N. and Thomas, D. N.}, title = {Dissolved organic matter studies in enclosed systems: Application of hydophobic fractionation for the assessment of organic nitrogen dynamics / Lara, R.J. ; Hubberten, U. ; Thomas, D.N. ; Baumann, M.E.M. ; Kattner, G.}, series = {Journal of Marine Systems. 13 (1997), H. 1-4}, journal = {Journal of Marine Systems. 13 (1997), H. 1-4}, isbn = {0924-7963}, pages = {155 -- 161}, year = {1997}, language = {en} } @article{BaumannTillmannAletsee1989, author = {Baumann, Marcus and Tillmann, Urban and Aletsee, Ludwig}, title = {Distribution of Carbon Among Photosynthetic End Products in the Bloom-Forming Arctic Diatom Thalassiosira antarctica COMBER / Tillmann, U. ; Baumann, M.E.M. ; Aletsee, L.}, series = {Polar Biology. 10 (1989), H. 3}, journal = {Polar Biology. 10 (1989), H. 3}, isbn = {0722-4060}, pages = {231 -- 238}, year = {1989}, language = {en} } @article{BaumannGradinger1991, author = {Baumann, Marcus and Gradinger, R.}, title = {Distribution of Phytoplankton Communities in Relation to the Large Scale Hydrographical Regime in the Fram Strait 1984 / Gradinger, R. ; Baumann, M.E.M.}, series = {Marine Biology. 111 (1991), H. 2}, journal = {Marine Biology. 111 (1991), H. 2}, isbn = {0025-3162}, pages = {311 -- 321}, year = {1991}, language = {en} } @article{BongaertsEsserLorbachetal.2011, author = {Bongaerts, Johannes and Esser, Simon and Lorbach, Volker and Al-Momani, L{\´o}ay and M{\"u}ller, Michael A. and Franke, Dirk and Grondal, Christoph and Kurutsch, Anja and Bujnicki, Robert and Takors, Ralf and Raeven, Leon and Wubbolts, Marcel and Bovenberg, Roel and Nieger, Martin and Sch{\"u}rmann, Melanie and Trachtmann, Natalie and Kozak, Stefan and Sprenger, Georg A. and M{\"u}ller, Michael}, title = {Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis}, series = {Angewandte Chemie International Edition}, volume = {Vol. 50}, journal = {Angewandte Chemie International Edition}, number = {Iss. 34}, publisher = {Wiley}, address = {Weinheim}, issn = {1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)}, pages = {7781 -- 7786}, year = {2011}, language = {en} } @article{SeiblerSchuebelerFieringetal.1998, author = {Seibler, Jost and Sch{\"u}beler, Dirk and Fiering, Steven and Groudine, Mark and Bode, J{\"u}rgen}, title = {DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs}, series = {Biochemistry}, volume = {37}, journal = {Biochemistry}, number = {18}, issn = {1520-4995}, doi = {10.1021/bi980288t}, pages = {6229 -- 6234}, year = {1998}, language = {en} } @article{SeiblerBode1997, author = {Seibler, Jost and Bode, J{\"u}rgen}, title = {Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay}, series = {Biochemistry}, volume = {36}, journal = {Biochemistry}, number = {7}, issn = {1520-4995}, pages = {1740 -- 1747}, year = {1997}, language = {en} }