@article{SiekerUlberDimitrovaetal.2009,
  author    = {Sieker, Tim and Ulber, Roland and Dimitrova, Darina and Bart, Hans-J{\"o}rg and Neuner, Andreas and Heinzle, Elmar and Tippk{\"o}tter, Nils},
  title     = {Silage : Fermentationsrohstoff f{\"u}r die chemische Industrie?},
  series = {labor\&more},
  journal   = {labor\&more},
  number    = {2},
  pages     = {44 -- 45},
  year      = {2009},
  abstract  = {In Anbetracht des zu erwartenden R{\"u}ckgangs der Verf{\"u}gbarkeit fossiler Rohstoffe m{\"u}ssen nicht nur f{\"u}r den Energiesektor, sondern auch f{\"u}r die Herstellung industrieller Produkte alternative Rohstoffe gefunden werden. Ein Beispiel f{\"u}r einen nicht in Nahrungsmittelkonkurrenz stehenden nachwachsenden Rohstoff ist gr{\"u}ne Biomasse wie Gras und Klee. Diese lassen sich in Deutschland auf großen Fl{\"a}chen anbauen und enthalten eine Vielzahl potenzieller Substrate f{\"u}r Fermentationen.},
  language  = {de}
}
@article{TippkoetterStueckmannKrolletal.2009,
  author    = {Tippk{\"o}tter, Nils and St{\"u}ckmann, Henning and Kroll, Stephen and Winkelmann, Gunda and Noack, Udo and Scheper, Thomas and Ulber, Roland},
  title     = {A semi-quantitative dipstick assay for microcystin},
  series = {Analytical and Bioanalytical Chemistry},
  volume    = {394},
  journal   = {Analytical and Bioanalytical Chemistry},
  number    = {3},
  publisher = {springer},
  address   = {Berlin},
  issn      = {1618-2650},
  doi       = {10.1007/s00216-009-2750-8},
  pages     = {863 -- 869},
  year      = {2009},
  abstract  = {An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.},
  language  = {en}
}
@article{UlberTippkoetter2009,
  author    = {Ulber, Roland and Tippk{\"o}tter, Nils},
  title     = {Nitratfreie Molke},
  series = {Rundschau f{\"u}r Fleischhygiene und Lebensmittel{\"u}berwachung},
  journal   = {Rundschau f{\"u}r Fleischhygiene und Lebensmittel{\"u}berwachung},
  number    = {4},
  pages     = {150 -- 152},
  year      = {2009},
  language  = {de}
}
@article{HendersonScheerWolf2009,
  author    = {Henderson, Colin J. and Scheer, Nico and Wolf, C. Roland},
  title     = {Advances in the generation of mouse models to elucidate the pathways of drug metabolism in rodents and man},
  series = {Expert Review of Clinical Pharmacology},
  volume    = {2},
  journal   = {Expert Review of Clinical Pharmacology},
  number    = {2},
  publisher = {Taylor \& Francis},
  address   = {London},
  issn      = {1751-2441},
  doi       = {10.1586/17512433.2.2.105},
  pages     = {105 -- 109},
  year      = {2009},
  language  = {en}
}
@article{StanleyHorsburghRossetal.2009,
  author    = {Stanley, Lesley A. and Horsburgh, Brian C. and Ross, Jillian and Scheer, Nico and Wolf, C. Roland},
  title     = {Drug transporters: Gatekeepers controlling access of xenobiotics to the cellular interior},
  series = {Drug Metabolism Reviews},
  volume    = {41},
  journal   = {Drug Metabolism Reviews},
  number    = {1},
  publisher = {Taylor \& Francis},
  address   = {London},
  issn      = {1097-9883},
  doi       = {10.1080/03602530802605040},
  pages     = {27 -- 65},
  year      = {2009},
  language  = {en}
}
@incollection{HendersonWolfScheer2009,
  author    = {Henderson, Colin J. and Wolf, C. Roland and Scheer, Nico},
  title     = {The use of transgenic animals to study drug metabolism},
  series = {Handbook of Drug Metabolism. 2nd Edition},
  booktitle = {Handbook of Drug Metabolism. 2nd Edition},
  editor    = {Woolf, Thomas F.},
  publisher = {Informa Healthcare},
  address   = {New York},
  isbn      = {978-1-4200-7647-9},
  pages     = {637 -- 658},
  year      = {2009},
  language  = {en}
}
@article{BalakrishnanAndreiSelmerSelmeretal.2010,
  author    = {Balakrishnan, Karthikeyan and Andrei-Selmer, Luminita-Cornelia and Selmer, Thorsten and Bacher, Michael and Dodel, Richard},
  title     = {Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β},
  series = {Journal of Alzheimer's Disease},
  volume    = {20},
  journal   = {Journal of Alzheimer's Disease},
  number    = {1},
  isbn      = {1387-2877},
  pages     = {135 -- 143},
  year      = {2010},
  language  = {en}
}
@misc{Jeromin2010,
  author    = {Jeromin, G{\"u}nter Erich},
  title     = {Immobilisierung von Alkoholdehydrogenasen und deren Coenzyme sowie Verwendung des Immobilisats : Offenlegungsschrift : DE 102008038326 A1 Offenlegungstag: 25.02.2010},
  publisher = {Deutsches Patent- und Markenamt},
  address   = {M{\"u}nchen},
  pages     = {6 S.},
  year      = {2010},
  language  = {de}
}
@article{KowollikSchnitzlerBisellietal.2010,
  author    = {Kowollik, S. and Schnitzler, Thomas and Biselli, Manfred and Krueger, R. and Zang, Werner and Peuscher, A. and Schillberg, S. and Fischer, R.},
  title     = {Die Rolle des Respirationsquotienten in der Zellkulturfermentation},
  series = {Chemie Ingenieur Technik},
  volume    = {82},
  journal   = {Chemie Ingenieur Technik},
  number    = {9},
  isbn      = {Chemie Ingenieur Tec},
  pages     = {1505 -- 1506},
  year      = {2010},
  language  = {de}
}
@article{BiselliBaeckerPoghossianetal.2010,
  author    = {Biselli, Manfred and B{\"a}cker, Matthias and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Schnitzler, Thomas and Zang, Werner and Wagner, P.},
  title     = {Entwicklung eines modularen festk{\"o}rperbasierten Sensorsystems f{\"u}r die {\"U}berwachung von Zellkulturfermenationen},
  series = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)},
  journal   = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)},
  publisher = {VDE Verlag},
  address   = {Berlin},
  isbn      = {978-3-8007-3260-9},
  pages     = {688 -- 691},
  year      = {2010},
  language  = {de}
}
@article{ClaessenGrefenMangetal.2010,
  author    = {Claessen, O. and Grefen, Dana and Mang, Thomas and Dikland, H. G. and Dikland, H. G. and Duin, M. van},
  title     = {Helle Fensterprofilmaterialien : Alterungsverhalten auf Basis von peroxidisch vernetztem EPDM},
  series = {Kautschuk, Gummi, Kunststoffe : KGK},
  volume    = {63},
  journal   = {Kautschuk, Gummi, Kunststoffe : KGK},
  number    = {9},
  isbn      = {0948-3276},
  pages     = {350 -- 360},
  year      = {2010},
  language  = {de}
}
@article{SrivastavaSinghAggarwaletal.2010,
  author    = {Srivastava, A. and Singh, V. and Aggarwal, P. and Schneeweiss, F. and Scherer, Ulrich W. and Friedrich, W.},
  title     = {Optical studies of insulating polymers for radiation dose monitoring},
  series = {Indian Journal of Pure \& Applied Physics},
  volume    = {48},
  journal   = {Indian Journal of Pure \& Applied Physics},
  number    = {11},
  isbn      = {0019-5596},
  pages     = {782 -- 786},
  year      = {2010},
  language  = {en}
}
@article{RibitschKarlBirnerGruenbergeretal.2010,
  author    = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.},
  title     = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli},
  series = {Journal of biotechnology},
  volume    = {150},
  journal   = {Journal of biotechnology},
  number    = {3},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1873-4863 (E-Journal); 0168-1656 (Print)},
  doi       = {10.1016/j.jbiotec.2010.09.947},
  pages     = {408 -- 416},
  year      = {2010},
  abstract  = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.},
  language  = {en}
}
@misc{SiegertMussmannO'Connelletal.2010,
  author    = {Siegert, Petra and Mussmann, Nina and O'Connell, Timothy and Maurer, Karl-Heinz},
  title     = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt / WIPO},
  address   = {M{\"u}nchen / Genf},
  pages     = {1 -- 30},
  year      = {2010},
  language  = {de}
}
@misc{SiegertBaumstarkKluinetal.2010,
  author    = {Siegert, Petra and Baumstark, Rebecca and Kluin, Cornelia and O'Connell, Timothy and Maurer, Karl-Heinz and Hellmuth, Hendrik},
  title     = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt},
  address   = {M{\"u}nchen},
  pages     = {1 -- 30},
  year      = {2010},
  language  = {de}
}
@misc{SiegertSpitzMaurer2010,
  author    = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz},
  title     = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]},
  publisher = {Deutsches Patentamt / WIPO},
  address   = {M{\"u}nchen / Genf},
  pages     = {1 -- 31},
  year      = {2010},
  language  = {de}
}
@misc{SiegertSpitzMaurer2010,
  author    = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz},
  title     = {Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus pumilus [Offenlegungsschrift]},
  publisher = {Deutsches Patentamt / Europ{\"a}isches Patentamt / WIPO},
  address   = {M{\"u}nchen / Den Hague / Genf},
  pages     = {1 -- 20},
  year      = {2010},
  language  = {de}
}
@misc{O'ConnellSiegertEversetal.2010,
  author    = {O'Connell, Timothy and Siegert, Petra and Evers, Stefan and Bongaerts, Johannes and Weber, Thomas and Maurer, Karl-Heinz and Bessler, Cornelius},
  title     = {Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft [Offenlegungsschrift]},
  publisher = {Deutsches Patentamt},
  address   = {M{\"u}nchen},
  pages     = {1 -- 34},
  year      = {2010},
  language  = {de}
}
@incollection{SeiblerSchwenk2010,
  author    = {Seibler, Jost and Schwenk, Frieder},
  title     = {Transgenic RNAi Applications in the Mouse},
  series = {Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition},
  booktitle = {Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition},
  publisher = {Elsevier},
  address   = {Amsterdam},
  isbn      = {978-0-12-384880-2},
  pages     = {367 -- 386},
  year      = {2010},
  language  = {en}
}
@misc{O'ConnellSiegertMaureretal.2010,
  author    = {O'Connell, Timothy and Siegert, Petra and Maurer, Karl-Heinz and Schiedel, Marc-Steffen and Vockenroth, Inga Kerstin},
  title     = {Method for improving the cleaning action of a detergent or cleaning agent [Internationale Patentanmeldung]},
  publisher = {WIPO},
  address   = {Genf},
  pages     = {1 -- 15},
  year      = {2010},
  language  = {en}
}