@inproceedings{KirchnerHenkelNaetheretal.2008, author = {Kirchner, Patrick and Henkel, H. and N{\"a}ther, Niko and Spelthahn, H. and Schneider, A. and Berger, J{\"o}rg and Kolstad, J. and Friedrich, P. and Sch{\"o}ning, Michael Josef}, title = {RFID-basiertes Sensorsystem zur Realisierung intelligenter Verpackungen f{\"u}r die Nahrungsmittelindustrie}, series = {KMU - innovativ: IKT 2008. CD-ROM : BMBF-Statustagung KMU - innovativ: IKT, Darmstadt, 17. - 18. Nov. 2008}, booktitle = {KMU - innovativ: IKT 2008. CD-ROM : BMBF-Statustagung KMU - innovativ: IKT, Darmstadt, 17. - 18. Nov. 2008}, number = {CD-ROM-Ausg.}, publisher = {BMBF}, address = {Berlin}, pages = {9 S.}, year = {2008}, language = {de} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @article{ReisertHenkelSchneideretal.2009, author = {Reisert, Steffen and Henkel, H. and Schneider, A. and Sch{\"a}fer, Daniel and Friedrich, P. and Berger, J{\"o}rg and Sch{\"o}ning, Michael Josef}, title = {Entwicklung eines Handheld-Sensorsystems f{\"u}r die „On-line"-Messung der H2O2-Konzentration in aseptischen Entkeimungsprozessen}, series = {9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]}, journal = {9. Dresdner Sensor-Symposium : Dresden, 07.-09. Dezember 2009 / Gerlach, Gerald ; Hauptmann, Peter [Hrsg.]}, publisher = {TUDpress}, address = {Dresden}, isbn = {978-3-941298-44-6}, pages = {285 -- 288}, year = {2009}, language = {de} } @article{RibitschHeumannKarletal.2012, author = {Ribitsch, D. and Heumann, S. and Karl, W. and Gerlach, J. and Leber, R. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Siegert, Petra and Lange, J. and Maurer, Karl-Heinz and Berg, G. and Guebitz, G. M. and Schwab, H.}, title = {Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli}, series = {Journal of biotechnology}, volume = {157}, journal = {Journal of biotechnology}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2011.09.025}, pages = {140 -- 147}, year = {2012}, abstract = {A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.}, language = {en} } @incollection{PoghossianSchusserBaeckeretal.2015, author = {Poghossian, Arshak and Schusser, Sebastian and B{\"a}cker, Matthias and Leinhos, Marcel and Sch{\"o}ning, Michael Josef}, title = {Real-time in-situ electrical monitoring of the degradation of biopolymers using semiconductor field-effect devices}, series = {Biodegradable biopolymers. Vol. 1}, booktitle = {Biodegradable biopolymers. Vol. 1}, publisher = {Nova Science Publ.}, address = {Hauppauge}, isbn = {978-1-63483-632-6}, pages = {135 -- 153}, year = {2015}, language = {en} } @inproceedings{BegingPoghossianMlyneketal.2010, author = {Beging, Stefan and Poghossian, Arshak and Mlynek, D. and Hataihimakul, S. and Pedraza, A. and Dhawan, S. and Laube, N. and Kleinen, Lisa and Baldsiefen, Gerhard and Busch, Heinrich von and Sch{\"o}ning, Michael Josef}, title = {Ion-selective sensors for the determination of the risk of urinary stone formation}, series = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, booktitle = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, address = {Warsaw}, pages = {74 -- 80}, year = {2010}, language = {en} } @article{BegingPoghossianSchoeningetal.2008, author = {Beging, Stefan and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Hataihimakul, Sudkanung and Busch, Heinz and Baldsiefen, Gerhard and Laube, N. and Kleinen, Lisa and Hosseiny, Reyhaneh}, title = {Feldeffektbasierender Ca2+-sensitiver Sensor f{\"u}r den Einsatz im Nativurin zur Bestimmung des Harnsteinbildungsrisikos}, series = {Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. M{\"a}rz 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, journal = {Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. M{\"a}rz 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, publisher = {VDI-Verl.}, address = {D{\"u}sseldorf}, isbn = {978-3-18-092011-5}, pages = {775 -- 782}, year = {2008}, language = {de} } @article{MiyamotoKanekoMatsuoetal.2010, author = {Miyamoto, Ko-ichiro and Kaneko, Kazumi and Matsuo, Akira and Wagner, Torsten and Kanoh, Shin`ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Miniaturized chemical imaging sensor system using an OLED display panel}, series = {Procedia Engineering}, volume = {5}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2010.09.160}, pages = {516 -- 519}, year = {2010}, abstract = {The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the two-dimensional distribution of specific ions or molecules in the solution. In this study, we developed a miniaturized chemical imaging sensor system with an OLED display panel as a light source that scans the sensor plate. In the proposed configuration, the display panel is placed directly below the sensor plate and illuminates the back surface. The measured area defined by illumination can be arbitrarily customized to fit the size and the shape of the sample to be measured. The waveform of the generated photocurrent, the currentvoltage characteristics and the pH sensitivity were investigated and pH imaging with this miniaturized system was demonstrated.}, language = {en} } @article{PoghossianWagnerSchoening2010, author = {Poghossian, Arshak and Wagner, Holger and Sch{\"o}ning, Michael Josef}, title = {Automatisiertes „wafer level"-Testsystem zur Charakterisierung von siliziumbasierten Chemo- und Biosensoren}, series = {Tagungsband: Sensoren und Messsysteme 2010}, journal = {Tagungsband: Sensoren und Messsysteme 2010}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {89 -- 92}, year = {2010}, abstract = {Es wurde ein automatisiertes, computerunterst{\"u}tztes Testsystem f{\"u}r die Funktionspr{\"u}fung und Charakterisierung von (bio-)chemischen Sensoren auf Waferebene entwickelt und in einen konventionellen Spitzenmessplatz integriert. Das System erm{\"o}glicht die Charakterisierung und Identifizierung „funktionstauglicher" Sensoren bereits auf Waferebene zwischen den einzelnen Herstellungsschritten, wodurch weitere, bisher {\"u}bliche Verarbeitungsschritte wie das Fixieren, Bonden und Verkapseln f{\"u}r die defekten oder nicht funktionstauglichen Sensorstrukturen entf{\"a}llt. Außerdem bietet eine speziell entworfene miniaturisierte Durchflussmesszelle die M{\"o}glichkeit, bereits auf Waferlevel die Sensitivit{\"a}t, Drift, Hysterese und Ansprechzeit der (bio-)chemischen Sensoren zu charakterisieren. Das System wurde exemplarisch mit kapazitiven, pH-sensitiven EIS- (Elektrolyt-Isolator-Silizium) Strukturen und ISFET- (ionensensitiver Feldeffekttransistor) Strukturen mit verschiedenen Geometrien und Gate-Layouts getestet.}, language = {de} } @article{KirchnerOberlaenderFriedrichetal.2010, author = {Kirchner, Patrick and Oberl{\"a}nder, Jan and Friedrich, Peter and Rysstad, Gunnar and Berger, J{\"o}rg and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Realization of a calorimetric gas sensor on polyimide foil for applications in aseptic food industry}, series = {Procedia Engineering}, volume = {5}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2010.09.098}, pages = {264 -- 267}, year = {2010}, abstract = {A calorimetric gas sensor is presented for the monitoring of gas-phase H2O2 at elevated temperature during sterilization processes in aseptic food industry. The sensor consists of two temperature-sensitive thin-film resistances built up on a polyimide foil with a thickness of 25 μm, which are passivated with a layer of SU-8 photo resist and catalytically activated with manganese(IV) oxide. Instead of an active heating structure, the calorimetric sensor utilizes the elevated temperature of an evaporated H2O2 aerosol. In an experimental set-up, the sensor has shown a sensitivity of 4.78 °C/(\%v/v) in a H2O2 concentration range of 0 to 10\% v/v at an evaporation temperature of 240 ∘C. Furthermore, the sensor possesses the same, unchanged sensor signal even at varied evaporation temperatures of the gas stream. The sensor characterization demonstrates the suitability of the calorimetric gas sensor for monitoring the efficiency of sterilization processes.}, language = {en} } @article{BohrnStuetzFleischeretal.2010, author = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef}, title = {Real-time detection of CO by eukaryotic cells}, series = {Procedia Engineering}, volume = {5}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, pages = {17 -- 20}, year = {2010}, abstract = {In this contribution, we focus on the detection of toxic gases with living eukaryotic cells. A cell-based gas sensor system, able to measure the effects of direct exposure of gases to cells in real-time, was set up. Impedance data as well as oxygen consumption of Chinese hamster lung fibroblast cells (V79) were analysed upon exposure to carbon monoxide (CO). The CO (diluted in wet synthetic air) affects the cell respiration as indicated by an attenuated respiration signal after the CO exposure as well as an instant increase of the capacitive part of the impedance signal during the gas exposure.}, language = {en} } @article{KirchnerSpelthahnSchoeningetal.2010, author = {Kirchner, Patrick and Spelthahn, Heiko and Sch{\"o}ning, Michael Josef and Henkel, Hartmut and Schneider, Andreas and Friedrich, Peter and Kolstad, Jens and Berger, J{\"o}rg}, title = {Realisierung eines Polyimid-basierten kalorimetrischen Gassensors zur Inline-{\"U}berwachung der H2O2-Konzentration in aseptischen Abf{\"u}llsystemen}, series = {Tagungsband: Sensoren und Messsysteme 2010}, journal = {Tagungsband: Sensoren und Messsysteme 2010}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {607 -- 612}, year = {2010}, abstract = {In aseptischen Abf{\"u}llsystemen wird Wasserstoffperoxid in der Gasphase aufgrund der stark oxidativen Wirkung zur Packstoffentkeimung eingesetzt. Dabei wird die Effizienz der Entkeimung im Wesentlichen von der vorliegenden H2O2-Konzentration im Packstoff bestimmt. Zur Inline-{\"U}berwachung der H2O2-Konzentration wurde ein kalorimetrischer Gassensor auf Basis einer flexiblen Polyimidfolie aus temperatursensitiven D{\"u}nnschicht-Widerst{\"a}nden und Mangan(IV)-oxid als katalytische Transducerschicht realisiert. Der Sensor weist ein lineares Ansprechverhalten mit einer Sensitivit{\"a}t von 7,15 °C/Vol.-\% in einem H2O2-Konzentrationsbereich von 0 bis 8 Vol.-\% auf. Weiterhin wurde zur Auslesung des Sensorsignals eine RFID-Elektronik, bestehend aus einem Sensor-Tag und einer Sende-/Empfangseinheit ausgelegt, sowie eine Abfolge des Messzyklus aufgestellt. Im weiteren Verlauf soll der kalorimetrische Gassensor mit der RFID-Elektronik gekoppelt und in eine Testverpackung zur Inline-{\"U}berwachung der H2O2-Konzentration in aseptischen Abf{\"u}llsystemen implementiert werden.}, language = {de} } @article{SpelthahnSchaffrathCoppeetal.2010, author = {Spelthahn, Heiko and Schaffrath, Sophie and Coppe, Thomas and Rufi, Frederic and Sch{\"o}ning, Michael Josef}, title = {Development of an electrolyte-insulator-semiconductor (EIS) based capacitive heavy metal sensor for the detection of Pb2+ und Cd2+ ions}, series = {Physica status solidi (a) : applications and material science}, volume = {207}, journal = {Physica status solidi (a) : applications and material science}, number = {4}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300}, doi = {10.1002/pssa.200983306}, pages = {930 -- 934}, year = {2010}, abstract = {Chalcogenide glass materials as membranes for potentiometric sensors for chemical analysis in solutions have been studied since more than 20 years. In this work, an electrolyte-insulator-semiconductor structure was combined with chalcogenide glass membranes prepared by means of the pulsed laser deposition technique. Depending on the membrane composition a selectivity to different ions (Cd2+ and Pb2+) is achieved. The different sensor membranes have been physically characterised using microscopy, ellipsometry, profilometry, atomic force microscopy (AFM), scanning electron microscopy (SEM) and Rutherford backscattering spectrometry (RBS). The electrochemical behaviour has been investigated via capacitance/voltage (C/V) and constant capacitance (ConCap) measurements and results in a Cd2+ sensitivity of 23.1 ± 0.6 mV per decade in a linear range from 7 × 10-6 to 10-2 mol/l and 24.4 ± 0.5 mV per decade in a linear range from 5 × 10-6 to 10-2 mol/l for Pb2+, respectively.}, language = {en} } @article{WernerSpelthahnSchoeningetal.2010, author = {Werner, Frederik and Spelthahn, Heiko and Sch{\"o}ning, Michael Josef and Krumbe, Christoph and Wagner, Torsten and Yoshinobu, Tatsuo and Keusgen, Michael}, title = {Neue Ansteuerungselektronik f{\"u}r LAPS-basierte Biosensoren zur gleichzeitig ortsaufgel{\"o}sten Messung der pH-Konzentration}, series = {Tagungsband: Sensoren und Messsysteme 2010}, journal = {Tagungsband: Sensoren und Messsysteme 2010}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {109 -- 114}, year = {2010}, abstract = {Ein lichtadressierbarer potentiometrischer Sensor (LAPS) kann die Konzentration eines oder mehrerer Analyten ortsaufgel{\"o}st auf der Sensoroberfl{\"a}che nachweisen. Dazu wird mit einer modulierten Lichtquelle die Halbleiterstruktur des zu untersuchenden Bereiches angeregt und ein entsprechender Photostrom ausgelesen. Durch gleichzeitige Anregung mehrere Bereiche durch Lichtquellen mit unterschiedlichen Modulationsfrequenzen k{\"o}nnen diese auch zeitgleich ausgelesen werden. Mit der neuen, hier vorgestellten Ansteuerungselektronik integriert in einem "Field Programmable Gate Array" (FPGA) ist es m{\"o}glich, mehrere Leuchtquellen gleichzeitig mit unterschiedlichen, w{\"a}hrend der Laufzeit festlegbaren Frequenzen, Phasen und Lichtintensit{\"a}ten zu betreiben. Somit kann das Frequenzverhalten des Sensors untersucht und die Konzentration des Analyten {\"u}ber das Oberfl{\"a}chenpotential mit Hilfe von Strom/Spannungs-Kurven und Phase/Spannungs-Kurven bestimmt werden.}, language = {de} } @article{WagnerMiyamotoSchoeningetal.2010, author = {Wagner, Torsten and Miyamoto, Ko-ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Novel combination of digital light processing (DLP) and light-addressable potentiometric sensors (LAPS) for flexible chemical imaging}, series = {Procedia Engineering}, volume = {5}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2010.09.161}, pages = {520 -- 523}, year = {2010}, abstract = {Chemical imaging systems allow the visualisation of the distribution of chemical species on the sensor surface. This work represents a new flexible approach of read out in a light-addressable potentiometric sensor (LAPS) with the help of a digital light processing (DLP) set-up. The DLP, known well for video projectors, consists of a mirror-array MEMS device which allows fast and flexible generation of light patterns. With the help of these light patterns the sensor surface of the LAPS device can be read out sequentially in a raster like scheme (scanning LAPS). The DLP approach has several advantages compared to conventional scanning LAPS set-ups, e.g., the spot size, the shape and the intensity of the light pointer can be changed easily and no mechanical movement is necessary, which reduces the size of the set-up and increases the stability and speed of measurement.}, language = {en} } @article{AbouzarPedrazaSchoeningetal.2010, author = {Abouzar, Maryam H. and Pedraza, A. M. and Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {Label-free DNA hybridization and denaturation detection by means of field-effect nanoplate SOI capacitors functionalized with gold nanoparticles}, series = {Procedia Engineering}, volume = {5}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2010.09.259}, pages = {918 -- 921}, year = {2010}, abstract = {A new approach for a label-free electrical detection of DNA hybridization and denaturation using an array of individually addressable field-effect nanoplate SOI (silicon-on-insulator) capacitors functionalized with gold nanoparticles is presented. By using a constant-capacitance measuring setup in a differential mode, signal changes of ∼110 mV and ∼70 mV have been registered after the DNA hybridization and denaturation events, respectively.}, language = {en} }