@article{NeumaierWeissVeldemanetal.2021,
  author    = {Neumaier, Felix and Weiss, Miriam and Veldeman, Michael and Kotliar, Konstantin and Wiesmann, Martin and Schulze-Steinen, Henna and H{\"o}llig, Anke and Clusmann, Hans and Schubert, Gerrit Alexander and Albanna, Walid},
  title     = {Changes in endogenous daytime melatonin levels after aneurysmal subarachnoid hemorrhage - preliminary findings from an observational cohort study},
  series = {Clinical Neurology and Neurosurgery},
  volume    = {208},
  journal   = {Clinical Neurology and Neurosurgery},
  number    = {Article No.: 106870},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0303-8467},
  doi       = {10.1016/j.clineuro.2021.106870},
  year      = {2021},
  abstract  = {Aneurysmal subarachnoid hemorrhage (aSAH) is associated with early and delayed brain injury due to several underlying and interrelated processes, which include inflammation, oxidative stress, endothelial, and neuronal apoptosis. Treatment with melatonin, a cytoprotective neurohormone with anti-inflammatory, anti-oxidant and anti-apoptotic effects, has been shown to attenuate early brain injury (EBI) and to prevent delayed cerebral vasospasm in experimental aSAH models. Less is known about the role of endogenous melatonin for aSAH outcome and how its production is altered by the pathophysiological cascades initiated during EBI. In the present observational study, we analyzed changes in melatonin levels during the first three weeks after aSAH.},
  language  = {en}
}
@article{BrockhausBehbahaniMurisetal.2021,
  author    = {Brockhaus, Moritz K. and Behbahani, Mehdi and Muris, Farina and Jansen, Sebastian V. and Schmitz- Rode, Thomas and Steinseifer, Ulrich and Clauser, Johanna C.},
  title     = {In vitro thrombogenicity testing of pulsatile mechanical circulatory support systems: Design and proof-of-concept},
  series = {Artificial Organs},
  volume    = {45},
  journal   = {Artificial Organs},
  number    = {12},
  publisher = {Wiley},
  address   = {Weinheim},
  issn      = {1525-1594},
  doi       = {10.1111/aor.14046},
  pages     = {1513 -- 1521},
  year      = {2021},
  abstract  = {Thrombogenic complications are a main issue in mechanical circulatory support (MCS). There is no validated in vitro method available to quantitatively assess the thrombogenic performance of pulsatile MCS devices under realistic hemodynamic conditions. The aim of this study is to propose a method to evaluate the thrombogenic potential of new designs without the use of complex in-vivo trials. This study presents a novel in vitro method for reproducible thrombogenicity testing of pulsatile MCS systems using low molecular weight heparinized porcine blood. Blood parameters are continuously measured with full blood thromboelastometry (ROTEM; EXTEM, FIBTEM and a custom-made analysis HEPNATEM). Thrombus formation is optically observed after four hours of testing. The results of three experiments are presented each with two parallel loops. The area of thrombus formation inside the MCS device was reproducible. The implantation of a filter inside the loop catches embolizing thrombi without a measurable increase of platelet activation, allowing conclusions of the place of origin of thrombi inside the device. EXTEM and FIBTEM parameters such as clotting velocity (α) and maximum clot firmness (MCF) show a total decrease by around 6\% with a characteristic kink after 180 minutes. HEPNATEM α and MCF rise within the first 180 minutes indicate a continuously increasing activation level of coagulation. After 180 minutes, the consumption of clotting factors prevails, resulting in a decrease of α and MCF. With the designed mock loop and the presented protocol we are able to identify thrombogenic hot spots inside a pulsatile pump and characterize their thrombogenic potential.},
  language  = {en}
}
@article{HacklBuessKammerlohretal.2021,
  author    = {Hackl, Michael and Buess, Eduard and Kammerlohr, Sandra and Nacov, Julia and Staat, Manfred and Leschinger, Tim and M{\"u}ller, Lars P. and Wegmann, Kilian},
  title     = {A "comma sign"-directed subscapularis repair in anterosuperior rotator cuff tears yields biomechanical advantages in a cadaveric model},
  series = {The american journal of sports medicine},
  volume    = {49},
  journal   = {The american journal of sports medicine},
  number    = {12},
  publisher = {Sage},
  address   = {London},
  issn      = {1552-3365},
  doi       = {10.1177/03635465211031506},
  pages     = {3212 -- 3217},
  year      = {2021},
  abstract  = {Background: Additional stabilization of the "comma sign" in anterosuperior rotator cuff repair has been proposed to provide biomechanical benefits regarding stability of the repair. Purpose: This in vitro investigation aimed to investigate the influence of a comma sign-directed reconstruction technique for anterosuperior rotator cuff tears on the primary stability of the subscapularis tendon repair. Study Design: Controlled laboratory study. Methods: A total of 18 fresh-frozen cadaveric shoulders were used in this study. Anterosuperior rotator cuff tears (complete full-thickness tear of the supraspinatus and subscapularis tendons) were created, and supraspinatus repair was performed with a standard suture bridge technique. The subscapularis was repaired with either a (1) single-row or (2) comma sign technique. A high-resolution 3D camera system was used to analyze 3-mm and 5-mm gap formation at the subscapularis tendon-bone interface upon incremental cyclic loading. Moreover, the ultimate failure load of the repair was recorded. A Mann-Whitney test was used to assess significant differences between the 2 groups. Results: The comma sign repair withstood significantly more loading cycles than the single-row repair until 3-mm and 5-mm gap formation occurred (P≤ .047). The ultimate failure load did not reveal any significant differences when the 2 techniques were compared (P = .596). Conclusion: The results of this study show that additional stabilization of the comma sign enhanced the primary stability of subscapularis tendon repair in anterosuperior rotator cuff tears. Although this stabilization did not seem to influence the ultimate failure load, it effectively decreased the micromotion at the tendon-bone interface during cyclic loading. Clinical Relevance: The proposed technique for stabilization of the comma sign has shown superior biomechanical properties in comparison with a single-row repair and might thus improve tendon healing. Further clinical research will be necessary to determine its influence on the functional outcome.},
  language  = {en}
}
@article{Staat2021,
  author    = {Staat, Manfred},
  title     = {An extension strain type Mohr-Coulomb criterion},
  series = {Rock mechanics and rock engineering},
  volume    = {54},
  journal   = {Rock mechanics and rock engineering},
  number    = {12},
  publisher = {Springer Nature},
  address   = {Cham},
  issn      = {1434-453X},
  doi       = {10.1007/s00603-021-02608-7},
  pages     = {6207 -- 6233},
  year      = {2021},
  abstract  = {Extension fractures are typical for the deformation under low or no confining pressure. They can be explained by a phenomenological extension strain failure criterion. In the past, a simple empirical criterion for fracture initiation in brittle rock has been developed. In this article, it is shown that the simple extension strain criterion makes unrealistic strength predictions in biaxial compression and tension. To overcome this major limitation, a new extension strain criterion is proposed by adding a weighted principal shear component to the simple criterion. The shear weight is chosen, such that the enriched extension strain criterion represents the same failure surface as the Mohr-Coulomb (MC) criterion. Thus, the MC criterion has been derived as an extension strain criterion predicting extension failure modes, which are unexpected in the classical understanding of the failure of cohesive-frictional materials. In progressive damage of rock, the most likely fracture direction is orthogonal to the maximum extension strain leading to dilatancy. The enriched extension strain criterion is proposed as a threshold surface for crack initiation CI and crack damage CD and as a failure surface at peak stress CP. Different from compressive loading, tensile loading requires only a limited number of critical cracks to cause failure. Therefore, for tensile stresses, the failure criteria must be modified somehow, possibly by a cut-off corresponding to the CI stress. Examples show that the enriched extension strain criterion predicts much lower volumes of damaged rock mass compared to the simple extension strain criterion.},
  language  = {en}
}
@article{TopcuMadabhushiStaat2022,
  author    = {Top{\c{c}}u, Murat and Madabhushi, Gopal S.P. and Staat, Manfred},
  title     = {A generalized shear-lag theory for elastic stress transfer between matrix and fibres having a variable radius},
  series = {International Journal of Solids and Structures},
  volume    = {239-240},
  journal   = {International Journal of Solids and Structures},
  number    = {Art. No. 111464},
  publisher = {Elsevier},
  address   = {New York, NY},
  issn      = {0020-7683},
  doi       = {10.1016/j.ijsolstr.2022.111464},
  year      = {2022},
  abstract  = {A generalized shear-lag theory for fibres with variable radius is developed to analyse elastic fibre/matrix stress transfer. The theory accounts for the reinforcement of biological composites, such as soft tissue and bone tissue, as well as for the reinforcement of technical composite materials, such as fibre-reinforced polymers (FRP). The original shear-lag theory proposed by Cox in 1952 is generalized for fibres with variable radius and with symmetric and asymmetric ends. Analytical solutions are derived for the distribution of axial and interfacial shear stress in cylindrical and elliptical fibres, as well as conical and paraboloidal fibres with asymmetric ends. Additionally, the distribution of axial and interfacial shear stress for conical and paraboloidal fibres with symmetric ends are numerically predicted. The results are compared with solutions from axisymmetric finite element models. A parameter study is performed, to investigate the suitability of alternative fibre geometries for use in FRP.},
  language  = {en}
}
@article{MandekarHollandThielenetal.2022,
  author    = {Mandekar, Swati and Holland, Abigail and Thielen, Moritz and Behbahani, Mehdi and Melnykowycz, Mark},
  title     = {Advancing towards Ubiquitous EEG, Correlation of In-Ear EEG with Forehead EEG},
  series = {Sensors},
  volume    = {22},
  journal   = {Sensors},
  number    = {4},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {1424-8220},
  doi       = {10.3390/s22041568},
  pages     = {1 -- 19},
  year      = {2022},
  abstract  = {Wearable EEG has gained popularity in recent years driven by promising uses outside of clinics and research. The ubiquitous application of continuous EEG requires unobtrusive form-factors that are easily acceptable by the end-users. In this progression, wearable EEG systems have been moving from full scalp to forehead and recently to the ear. The aim of this study is to demonstrate that emerging ear-EEG provides similar impedance and signal properties as established forehead EEG. EEG data using eyes-open and closed alpha paradigm were acquired from ten healthy subjects using generic earpieces fitted with three custom-made electrodes and a forehead electrode (at Fpx) after impedance analysis. Inter-subject variability in in-ear electrode impedance ranged from 20 kΩ to 25 kΩ at 10 Hz. Signal quality was comparable with an SNR of 6 for in-ear and 8 for forehead electrodes. Alpha attenuation was significant during the eyes-open condition in all in-ear electrodes, and it followed the structure of power spectral density plots of forehead electrodes, with the Pearson correlation coefficient of 0.92 between in-ear locations ELE (Left Ear Superior) and ERE (Right Ear Superior) and forehead locations, Fp1 and Fp2, respectively. The results indicate that in-ear EEG is an unobtrusive alternative in terms of impedance, signal properties and information content to established forehead EEG.},
  language  = {en}
}
@article{DefosseKleinschmidtSchmutzetal.2022,
  author    = {Defosse, Jerome and Kleinschmidt, Joris and Schmutz, Axel and Loop, Torsten and Staat, Manfred and Gatzweiler, Karl-Heinz and Wappler, Frank and Schieren, Mark},
  title     = {Dental strain on maxillary incisors during tracheal intubation with double-lumen tubes and different laryngoscopy techniques - a blinded manikin study},
  series = {Journal of Cardiothoracic and Vascular Anesthesia},
  volume    = {36},
  journal   = {Journal of Cardiothoracic and Vascular Anesthesia},
  number    = {8, Part B},
  publisher = {Elsevier},
  address   = {New York, NY},
  issn      = {1053-0770},
  doi       = {10.1053/j.jvca.2022.02.017},
  pages     = {3021 -- 3027},
  year      = {2022},
  language  = {en}
}
@article{BhattaraiMayStaatetal.2022,
  author    = {Bhattarai, Aroj and May, Charlotte Anabell and Staat, Manfred and Kowalczyk, Wojciech and Tran, Thanh Ngoc},
  title     = {Layer-specific damage modeling of porcine large intestine under biaxial tension},
  series = {Bioengineering},
  volume    = {9},
  journal   = {Bioengineering},
  number    = {10, Early Access},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2306-5354},
  doi       = {10.3390/bioengineering9100528},
  pages     = {1 -- 17},
  year      = {2022},
  abstract  = {The mechanical behavior of the large intestine beyond the ultimate stress has never been investigated. Stretching beyond the ultimate stress may drastically impair the tissue microstructure, which consequently weakens its healthy state functions of absorption, temporary storage, and transportation for defecation. Due to closely similar microstructure and function with humans, biaxial tensile experiments on the porcine large intestine have been performed in this study. In this paper, we report hyperelastic characterization of the large intestine based on experiments in 102 specimens. We also report the theoretical analysis of the experimental results, including an exponential damage evolution function. The fracture energies and the threshold stresses are set as damage material parameters for the longitudinal muscular, the circumferential muscular and the submucosal collagenous layers. A biaxial tensile simulation of a linear brick element has been performed to validate the applicability of the estimated material parameters. The model successfully simulates the biomechanical response of the large intestine under physiological and non-physiological loads.},
  language  = {en}
}
@article{ColomboDriraFrotscheretal.2022,
  author    = {Colombo, Daniele and Drira, Slah and Frotscher, Ralf and Staat, Manfred},
  title     = {An element-based formulation for ES-FEM and FS-FEM models for implementation in standard solid mechanics finite element codes for 2D and 3D static analysis},
  series = {International Journal for Numerical Methods in Engineering},
  volume    = {124},
  journal   = {International Journal for Numerical Methods in Engineering},
  number    = {2},
  publisher = {Wiley},
  address   = {Chichester},
  issn      = {1097-0207},
  doi       = {10.1002/nme.7126},
  pages     = {402 -- 433},
  year      = {2022},
  abstract  = {Edge-based and face-based smoothed finite element methods (ES-FEM and FS-FEM, respectively) are modified versions of the finite element method allowing to achieve more accurate results and to reduce sensitivity to mesh distortion, at least for linear elements. These properties make the two methods very attractive. However, their implementation in a standard finite element code is nontrivial because it requires heavy and extensive modifications to the code architecture. In this article, we present an element-based formulation of ES-FEM and FS-FEM methods allowing to implement the two methods in a standard finite element code with no modifications to its architecture. Moreover, the element-based formulation permits to easily manage any type of element, especially in 3D models where, to the best of the authors' knowledge, only tetrahedral elements are used in FS-FEM applications found in the literature. Shape functions for non-simplex 3D elements are proposed in order to apply FS-FEM to any standard finite element.},
  language  = {en}
}
@article{KotliarOrtnerConradietal.2022,
  author    = {Kotliar, Konstantin and Ortner, Marion and Conradi, Anna and Hacker, Patricia and Hauser, Christine and G{\"u}nthner, Roman and Moser, Michaela and Muggenthaler, Claudia and Diehl-Schmid, Janine and Priller, Josef and Schmaderer, Christoph and Grimmer, Timo},
  title     = {Altered retinal cerebral vessel oscillation frequencies in Alzheimer's disease compatible with impaired amyloid clearance},
  series = {Neurobiology of Aging},
  volume    = {120},
  journal   = {Neurobiology of Aging},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0197-4580},
  doi       = {10.1016/j.neurobiolaging.2022.08.012},
  pages     = {117 -- 127},
  year      = {2022},
  abstract  = {Retinal vessels are similar to cerebral vessels in their structure and function. Moderately low oscillation frequencies of around 0.1 Hz have been reported as the driving force for paravascular drainage in gray matter in mice and are known as the frequencies of lymphatic vessels in humans. We aimed to elucidate whether retinal vessel oscillations are altered in Alzheimer's disease (AD) at the stage of dementia or mild cognitive impairment (MCI). Seventeen patients with mild-to-moderate dementia due to AD (ADD); 23 patients with MCI due to AD, and 18 cognitively healthy controls (HC) were examined using Dynamic Retinal Vessel Analyzer. Oscillatory temporal changes of retinal vessel diameters were evaluated using mathematical signal analysis. Especially at moderately low frequencies around 0.1 Hz, arterial oscillations in ADD and MCI significantly prevailed over HC oscillations and correlated with disease severity. The pronounced retinal arterial vasomotion at moderately low frequencies in the ADD and MCI groups would be compatible with the view of a compensatory upregulation of paravascular drainage in AD and strengthen the amyloid clearance hypothesis.},
  language  = {en}
}
@article{BayerTemizArtmannDigeletal.2020,
  author    = {Bayer, Robin and Temiz Artmann, Ayseg{\"u}l and Digel, Ilya and Falkenstein, Julia and Artmann, Gerhard and Creutz, Till and Hescheler, J{\"u}rgen},
  title     = {Mechano-pharmacological testing of L-Type Ca²⁺ channel modulators via human vascular celldrum model},
  series = {Cellular Physiology and Biochemistry},
  volume    = {54},
  journal   = {Cellular Physiology and Biochemistry},
  publisher = {Cell Physiol Biochem Press},
  address   = {D{\"u}sseldorf},
  issn      = {1421-9778},
  doi       = {10.33594/000000225},
  pages     = {371 -- 383},
  year      = {2020},
  abstract  = {Background/Aims: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology. Methods: The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. The cell generated tensions deviations in the range of a few N/m² are evaluated by the CellDrum technology. This study focuses on the dilative and contractive effects of L-type Ca²⁺ channel agonists and antagonists, respectively. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate analysis technique for the study purpose. These three operation modes are called, particular time mode" (PTM), "long term mode" (LTM) and "real-time mode" (RTM). Results: It was possible to quantify the biomechanical response of haSMCs to the addition of vasoactive agents using CellDrum technology. Due to the supplementation of 100nM Bay K8644, the tension increased approximately 10.6\% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomechanical stress around 6,5\% and 50nM verapamil by 2,8\%, compared to the initial tension maximum. Additionally, all tested measurement modes provide similar results while focusing on different analysis parameters. Conclusion: The CellDrum technology allows highly sensitive biomechanical stress measurements of cultured haSMC monolayers. The mechanical stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m²). All tested operation modes resulted in equal findings, whereas each mode features operation-related data analysis.},
  language  = {en}
}
@article{AkimbekovDigelAbdievaetal.2021,
  author    = {Akimbekov, Nuraly and Digel, Ilya and Abdieva, Gulzhamal and Ualieva, Perizat and Tastambek, Kuanysh},
  title     = {Lignite biosolubilization and bioconversion by Bacillus sp.: the collation of analytical data},
  series = {Biofuels},
  volume    = {12},
  journal   = {Biofuels},
  number    = {3},
  publisher = {Taylor \& Francis},
  address   = {London},
  issn      = {1759-7277},
  pages     = {247 -- 258},
  year      = {2021},
  abstract  = {The vast metabolic potential of microbes in brown coal (lignite) processing and utilization can greatly contribute to innovative approaches to sustainable production of high-value products from coal. In this study, the multi-faceted and complex coal biosolubilization process by Bacillus sp. RKB 7 isolate from the Kazakhstan coal-mining soil is reported, and the derived products are characterized. Lignite solubilization tests performed for surface and suspension cultures testify to the formation of numerous soluble lignite-derived substances. Almost 24\% of crude lignite (5\% w/v) was solubilized within 14 days under slightly alkaline conditions (pH 8.2). FTIR analysis revealed various functional groups in the obtained biosolubilization products. Analyses of the lignite-derived humic products by UV-Vis and fluorescence spectrometry as well as elemental analysis yielded compatible results indicating the emerging products had a lower molecular weight and degree of aromaticity. Furthermore, XRD and SEM analyses were used to evaluate the biosolubilization processes from mineralogical and microscopic points of view. The findings not only contribute to a deeper understanding of microbe-mineral interactions in coal environments, but also contribute to knowledge of coal biosolubilization and bioconversion with regard to sustainable production of humic substances. The detailed and comprehensive analyses demonstrate the huge biotechnological potential of Bacillus sp. for agricultural productivity and environmental health.},
  language  = {en}
}
@article{QuittmannAbelAlbrachtetal.2020,
  author    = {Quittmann, Oliver J. and Abel, Thomas and Albracht, Kirsten and Meskemper, Joshua and Foitschik, Tina and Str{\"u}der, Heiko K.},
  title     = {Biomechanics of handcycling propulsion in a 30-min continuous load test at lactate threshold: Kinetics, kinematics, and muscular activity in able-bodied participants},
  series = {European Journal of Applied Physiology},
  journal   = {European Journal of Applied Physiology},
  number    = {120},
  publisher = {Springer},
  address   = {Heidelberg},
  issn      = {1439-6327},
  doi       = {10.1007/s00421-020-04373-x},
  pages     = {1403 -- 1415},
  year      = {2020},
  abstract  = {Purpose This study aims to investigate the biomechanics of handcycling during a continuous load trial (CLT) to assess the mechanisms underlying fatigue in upper body exercise. Methods Twelve able-bodied triathletes performed a 30-min CLT at a power output corresponding to lactate threshold in a racing recumbent handcycle mounted on a stationary ergometer. During the CLT, ratings of perceived exertion (RPE), tangential crank kinetics, 3D joint kinematics, and muscular activity of ten muscles of the upper extremity and trunk were examined using motion capturing and surface electromyography. Results During the CLT, spontaneously chosen cadence and RPE increased, whereas crank torque decreased. Rotational work was higher during the pull phase. Peripheral RPE was higher compared to central RPE. Joint range of motion decreased for elbow-flexion and radial-duction. Integrated EMG (iEMG) increased in the forearm flexors, forearm extensors, and M. deltoideus (Pars spinalis). An earlier onset of activation was found for M. deltoideus (Pars clavicularis), M. pectoralis major, M. rectus abdominis, M. biceps brachii, and the forearm flexors. Conclusion Fatigue-related alterations seem to apply analogously in handcycling and cycling. The most distal muscles are responsible for force transmission on the cranks and might thus suffer most from neuromuscular fatigue. The findings indicate that peripheral fatigue (at similar lactate values) is higher in handcycling compared to leg cycling, at least for inexperienced participants. An increase in cadence might delay peripheral fatigue by a reduced vascular occlusion. We assume that the gap between peripheral and central fatigue can be reduced by sport-specific endurance training.},
  language  = {en}
}
@article{KnoxBruggemannGossmannetal.2020,
  author    = {Knox, Ronald and Bruggemann, Andrea and Gossmann, Matthias and Thomas, Ulrich and Horv{\´a}th, Andr{\´a}s and Dragicevic, Elena and Stoelzle-Feix, Sonja and Fertig, Niels and Jung, Alexander and Raman, Aravind Hariharan and Staat, Manfred and Linder, Peter},
  title     = {Combining physiological relevance and throughput for in vitro cardiac contractility measurement},
  series = {Biophysical Journal},
  volume    = {118},
  journal   = {Biophysical Journal},
  number    = {Issue 3, Supplement 1},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0006-3495},
  doi       = {10.1016/j.bpj.2019.11.3104},
  pages     = {570a},
  year      = {2020},
  abstract  = {Despite increasing acceptance of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) in safety pharmacology, controversy remains about the physiological relevance of existing in vitro models for their mechanical testing. We hypothesize that existing signs of immaturity of the cell models result from an improper mechanical environment. We cultured hiPSC-CMs in a 96-well format on hyperelastic silicone membranes imitating their native mechanical environment, resulting in physiological responses to compound stimuli.We validated cell responses on the FLEXcyte 96, with a set of reference compounds covering a broad range of cellular targets, including ion channel modulators, adrenergic receptor modulators and kinase inhibitors. Acute (10 - 30 min) and chronic (up to 7 days) effects were investigated. Furthermore, the measurements were complemented with electromechanical models based on electrophysiological recordings of the used cell types.hiPSC-CMs were cultured on freely-swinging, ultra-thin and hyperelastic silicone membranes. The weight of the cell culture medium deflects the membranes downwards. Rhythmic contraction of the hiPSC-CMs resulted in dynamic deflection changes which were quantified by capacitive distance sensing. The cells were cultured for 7 days prior to compound addition. Acute measurements were conducted 10-30 minutes after compound addition in standard culture medium. For chronic treatment, compound-containing medium was replaced daily for up to 7 days. Electrophysiological properties of the employed cell types were recorded by automated patch-clamp (Patchliner) and the results were integrated into the electromechanical model of the system.Calcium channel agonist S Bay K8644 and beta-adrenergic stimulator isoproterenol induced significant positive inotropic responses without additional external stimulation. Kinase inhibitors displayed cardiotoxic effects on a functional level at low concentrations. The system-integrated analysis detected alterations in beating shape as well as frequency and arrhythmic events and we provide a quantitative measure of these.},
  language  = {en}
}
@article{ErmolaevNivokovMelnikovaetal.2018,
  author    = {Ermolaev, A.P. and Nivokov, I.A. and Melnikova, L.I. and Kotliar, Konstantin},
  title     = {Сравнительная характеристика химического состава витреального содержимого кадаверных глаз и глаз с рефрактерной терминальной глаукомой},
  series = {Vestnik oftalmologii},
  volume    = {5},
  journal   = {Vestnik oftalmologii},
  number    = {2},
  publisher = {Media Sfera},
  address   = {Moskau},
  doi       = {10.17116/oftalma2018134051195},
  pages     = {195 -- 201},
  year      = {2018},
  abstract  = {Purpose — to compare the chemical elemental composition of vitreous cavity content taken from cadaveric eyes compared to samples taken from the eyes with terminal stage refractory glaucoma with decompensated intraocular pressure (IOP). Material and methods. The vitreous contents of the eyes from 2 groups were studied. The 1st group included 15 cadaveric eyes; the 2nd group included 15 eyes with refractory glaucoma in the terminal stage of the disease with decompensated IOP in patients with hypertension pain. The vitreal content samples were taken in the course of antiglaucoma surgery aimed at preserving the eye as an organ and involving employment of drainage in the vitreous cavity. The study of virtual contents was carried out on energy dispersive spectrometer Oxford X-Max 50 integrated into scanning electron microscope Zeiss EVO LS10. Results. Increased concentrations of Kalium and Phosphorus were detected in the vitreous content of cadaveric eyes compared with the vitreal content from the eyes with terminal glaucoma with decompensated IOP taken in vivo (K — 0.172/0.093; P — 0.045/0.025 mmol/L). In the vitreous cavity in the eyes with end-stage glaucoma with decompensated IOP, the concentration of Nitrogen was higher in comparison with human cadaver eyes (2.030/1.424 mmol/L). Conclusion. The increased concentrations of Kalium and Phosphorus in the vitreous content of cadaveric eyes is associated with postmortem autolytic processes and with the release of intracellular content in the destruction of cell membranes. The increased Nitrogen concentration in the vitreal contents of the eyes with terminal stage glaucoma with decompensated IOP may be associated with the presence of osmotically active nitrogen-containing compounds in the eyes with increased IOP.},
  language  = {ru}
}
@article{WerkhausenCroninAlbrachtetal.2019,
  author    = {Werkhausen, Amelie and Cronin, Neil J. and Albracht, Kirsten and Bojsen-M{\o}ller, Jens and Seynnes, Olivier R.},
  title     = {Distinct muscle-tendon interaction during running at different speeds and in different loading conditions},
  series = {Journal of Applied Physiology},
  volume    = {127},
  journal   = {Journal of Applied Physiology},
  number    = {1},
  issn      = {1522-1601},
  doi       = {10.1152/japplphysiol.00710.2018},
  pages     = {246 -- 253},
  year      = {2019},
  language  = {en}
}
@article{AttarMerkKotliaretal.2019,
  author    = {Attar, Mandana Hossein Zadeh and Merk, Hans F. and Kotliar, Konstantin and Wurpts, Gerda and R{\"o}seler, Stefani and Moll-Slodowy, Silke and Plange, Johann and Baron, Jens Malte and Balakirski, Galina},
  title     = {The CD63 basophil activation test as a diagnostic tool for assessing autoimmunity in patients with chronic spontaneous urticaria},
  series = {European Journal of Dermatology},
  volume    = {29},
  journal   = {European Journal of Dermatology},
  number    = {6},
  doi       = {10.1684/ejd.2019.3680},
  pages     = {614 -- 618},
  year      = {2019},
  language  = {en}
}
@article{MalikAbdievaUalievaetal.2019,
  author    = {Malik, A. M. and Abdieva, G. Zh. and Ualieva, P. S. and Zhubanova, A. A. and Temiz Artmann, Ayseg{\"u}l},
  title     = {CКPИНИНГ МИКPOOPГAНИЗМOВ-ДECТPУКТOPOВ XЛOРOPГAНИЧECКИX ЗAГPЯЗНИТEЛEЙ},
  series = {Eurasian Journal of Ecology},
  volume    = {61},
  journal   = {Eurasian Journal of Ecology},
  number    = {4},
  issn      = {2617-7358},
  pages     = {61 -- 71},
  year      = {2019},
  language  = {ru}
}
@article{SmithKotliarLammertynetal.2020,
  author    = {Smith, Wayne and Kotliar, Konstantin and Lammertyn, Leandi and Ramoshaba, Nthai E. and Vilser, Walthard and Huisman, Hugo W. and Schutte, Aletta E.},
  title     = {Retinal vessel caliber and caliber responses in true normotensive black and white adults: The African-PREDICT study},
  series = {Microvascular Research},
  volume    = {128},
  journal   = {Microvascular Research},
  number    = {Article 103937},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {0026-2862},
  doi       = {10.1016/j.mvr.2019.103937},
  year      = {2020},
  abstract  = {Purpose Globally, a detrimental shift in cardiovascular disease risk factors and a higher mortality level are reported in some black populations. The retinal microvasculature provides early insight into the pathogenesis of systemic vascular diseases, but it is unclear whether retinal vessel calibers and acute retinal vessel functional responses differ between young healthy black and white adults. Methods We included 112 black and 143 white healthy normotensive adults (20-30 years). Retinal vessel calibers (central retinal artery and vein equivalent (CRAE and CRVE)) were calculated from retinal images and vessel caliber responses to flicker light induced provocation (FLIP) were determined. Additionally, ambulatory blood pressure (BP), anthropometry and blood samples were collected. Results The groups displayed similar 24 h BP profiles and anthropometry (all p > .24). Black participants demonstrated a smaller CRAE (158 ± 11 vs. 164 ± 11 MU, p < .001) compared to the white group, whereas CRVE was similar (p = .57). In response to FLIP, artery maximal dilation was greater in the black vs. white group (5.6 ± 2.1 vs. 3.3 ± 1.8\%; p < .001). Conclusions Already at a young age, healthy black adults showed narrower retinal arteries relative to the white population. Follow-up studies are underway to show if this will be related to increased risk for hypertension development. The reason for the larger vessel dilation responses to FLIP in the black population is unclear and warrants further investigation.},
  language  = {en}
}
@article{StreeseKotliarDeiserothetal.2020,
  author    = {Streese, Lukas and Kotliar, Konstantin and Deiseroth, Arne and Infanger, Denis and Gugleta, Konstantin and Schmaderer, Christoph and Hanssen, Henner},
  title     = {Retinal endothelial function in cardiovascular risk patients: A randomized controlled exercise trial},
  series = {Scandinavian Journal of Medicine and Science in Sports},
  volume    = {30},
  journal   = {Scandinavian Journal of Medicine and Science in Sports},
  number    = {2},
  publisher = {Wiley},
  address   = {Oxford},
  issn      = {1600-0838},
  doi       = {10.1111/sms.13560},
  pages     = {272 -- 280},
  year      = {2020},
  abstract  = {The aim of this study was to investigate, for the first time, the effects of high-intensity interval training (HIIT) on retinal microvascular endothelial function in cardiovascular (CV) risk patients. In the randomized controlled trial, middle-aged and previously sedentary patients with increased CV risk (aged 58 ± 6 years) with ≥ two CV risk factors were randomized into a 12-week HIIT (n = 33) or control group (CG, n = 36) with standard physical activity recommendations. A blinded examiner measured retinal endothelial function by flicker light-induced maximal arteriolar (ADmax) and venular (VDmax) dilatation as well as the area under the arteriolar (AFarea) and venular (VFarea) flicker curve using a retinal vessel analyzer. Standardized assessments of CV risk factors, cardiorespiratory fitness, and retinal endothelial function were performed before and after HIIT. HIIT reduced body mass index, fat mass, and low-density lipoprotein and increased muscle mass and peak oxygen uptake (VO2peak). Both ADmax (pre: 2.7 ± 2.1\%, post: 3.0 ± 2.2\%, P = .018) and AFarea (pre: 32.6 ± 28.4\%*s, post: 37.7 ± 30.6\%*s, P = .016) increased after HIIT compared with CG (ADmax, pre: 3.2 ± 1.8\%, post: 2.9 ± 1.8\%, P = .254; AFarea, pre: 41.6 ± 28.5\%*s, post: 37.8 ± 27.0\%*s, P = .186). Venular function remained unchanged after HIIT. There was a significant association between ∆-change VO2peak and ∆-changes ADmax and AFarea (P = .026, R² = 0.073; P = .019, R² = 0.081, respectively). 12-weeks of HIIT improved retinal endothelial function in middle-aged patients with increased CV risk independent of the reduction in classical CV risk factors. Exercise has the potential to reverse or at least postpone progression of small vessel disease in older adults with increased CV risk under standard medication. Dynamic retinal vessel analysis seems to be a sensitive tool to detect treatment effects of exercise interventions on retinal microvascular endothelial function in middle-aged individuals with increased CV risk.},
  language  = {en}
}