@article{BegingMlynekHataihimakuletal.2010, author = {Beging, Stefan and Mlynek, Daniela and Hataihimakul, Sudkanung and Poghossian, Arshak and Baldsiefen, Gerhard and Busch, Heinz and Laube, Norbert and Kleinen, Lisa and Sch{\"o}ning, Michael Josef}, title = {Field-effect calcium sensor for the determination of the risk of urinary stone formation}, series = {Sensors and Actuators B: Chemical. 144 (2010), H. 2}, journal = {Sensors and Actuators B: Chemical. 144 (2010), H. 2}, pages = {374 -- 379}, year = {2010}, language = {en} } @inproceedings{BegingPoghossianMlyneketal.2010, author = {Beging, Stefan and Poghossian, Arshak and Mlynek, D. and Hataihimakul, S. and Pedraza, A. and Dhawan, S. and Laube, N. and Kleinen, L. and Baldsiefen, G. and Busch, H. and Sch{\"o}ning, Michael Josef}, title = {Ion-selective sensors for the determination of the risk of urinary stone formation}, series = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, booktitle = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, address = {Warsaw}, pages = {74 -- 80}, year = {2010}, language = {en} } @article{BegingPoghossianSchoeningetal.2008, author = {Beging, Stefan and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Hataihimakul, Sudkanung and Busch, H. and Baldsiefen, G. and Laube, N. and Kleinen, L. and Hosseiny, R.}, title = {Feldeffektbasierender Ca2+-sensitiver Sensor f{\"u}r den Einsatz im Nativurin zur Bestimmung des Harnsteinbildungsrisikos}, series = {Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. M{\"a}rz 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, journal = {Sensoren und Messsysteme 2008 : 14. Fachtagung Ludwigsburg, 11. und 12. M{\"a}rz 2008 / VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, publisher = {VDI-Verl.}, address = {D{\"u}sseldorf}, isbn = {978-3-18-092011-5}, pages = {775 -- 782}, year = {2008}, language = {de} } @misc{BergerBiselliDinteretal.2000, author = {Berger, Eric G. and Biselli, Manfred and Dinter, Andr{\´e} and Eisenkr{\"a}tzer, Detlef and Kiesewetter, Andr{\´e} and Zeng, Steffen}, title = {Chinese-Hamster-Ovary-Zellen zur Produktion von Proteinen}, pages = {1 -- 6}, year = {2000}, language = {de} } @article{BertzMolinnusSchoeningetal.2023, author = {Bertz, Morten and Molinnus, Denise and Sch{\"o}ning, Michael Josef and Homma, Takayuki}, title = {Real-time monitoring of H₂O₂ sterilization on individual bacillus atrophaeus spores by optical sensing with trapping Raman spectroscopy}, series = {Chemosensors}, volume = {8}, journal = {Chemosensors}, number = {11}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors11080445}, pages = {Artikel 445}, year = {2023}, abstract = {Hydrogen peroxide (H₂O₂), a strong oxidizer, is a commonly used sterilization agent employed during aseptic food processing and medical applications. To assess the sterilization efficiency with H₂O₂, bacterial spores are common microbial systems due to their remarkable robustness against a wide variety of decontamination strategies. Despite their widespread use, there is, however, only little information about the detailed time-resolved mechanism underlying the oxidative spore death by H₂O₂. In this work, we investigate chemical and morphological changes of individual Bacillus atrophaeus spores undergoing oxidative damage using optical sensing with trapping Raman microscopy in real-time. The time-resolved experiments reveal that spore death involves two distinct phases: (i) an initial phase dominated by the fast release of dipicolinic acid (DPA), a major spore biomarker, which indicates the rupture of the spore's core; and (ii) the oxidation of the remaining spore material resulting in the subsequent fragmentation of the spores' coat. Simultaneous observation of the spore morphology by optical microscopy corroborates these mechanisms. The dependence of the onset of DPA release and the time constant of spore fragmentation on H₂O₂ shows that the formation of reactive oxygen species from H₂O₂ is the rate-limiting factor of oxidative spore death.}, language = {en} } @article{BertzSchoeningMolinnusetal.2024, author = {Bertz, Morten and Sch{\"o}ning, Michael Josef and Molinnus, Denise and Homma, Takayuki}, title = {Influence of temperature, light, and H₂O₂ concentration on microbial spore inactivation: in-situ Raman spectroscopy combined with optical trapping}, series = {Physica status solidi (a) applications and materials science}, journal = {Physica status solidi (a) applications and materials science}, number = {Early View}, publisher = {Wiley-VCH}, address = {Berlin}, issn = {1862-6319 (Online)}, doi = {10.1002/pssa.202300866}, pages = {8 Seiten}, year = {2024}, abstract = {To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30\% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.}, language = {en} } @article{BiselliBaeckerPoghossianetal.2010, author = {Biselli, Manfred and B{\"a}cker, Matthias and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Schnitzler, Thomas and Zang, Werner and Wagner, P.}, title = {Entwicklung eines modularen festk{\"o}rperbasierten Sensorsystems f{\"u}r die {\"U}berwachung von Zellkulturfermenationen}, series = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, journal = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {688 -- 691}, year = {2010}, language = {de} } @article{BohrnStuetzFleischeretal.2010, author = {Bohrn, U. and St{\"u}tz, E. and Fleischer, M. and Sch{\"o}ning, Michael Josef}, title = {Real-time detection of CO by eukaryotic cells}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {17 -- 20}, year = {2010}, language = {en} } @inproceedings{BohrnMuchaWerneretal.2012, author = {Bohrn, Ulrich and Mucha, Andreas and Werner, Frederik and St{\"u}tz, Evamaria and B{\"a}cker, Matthias and Krumbe, Christoph and Schienle, Meinrad and Fleischer, Maximilian and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Detection of toxic chromium species in water using cellbased sensor systems}, isbn = {978-3-9813484-2-2}, doi = {10.5162/IMCS2012/P2.1.14}, pages = {1364 -- 1367}, year = {2012}, language = {en} } @article{BohrnMuchaWerneretal.2013, author = {Bohrn, Ulrich and Mucha, Andreas and Werner, Frederik and Trattner, Barbara and B{\"a}cker, Matthias and Krumbe, Christoph and Schienle, Meinrad and St{\"u}tz, Evamaria and Schmitt-Landsiedel, Doris and Fleischer, Maximilian and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {A critical comparison of cell-based sensor systems for the detection of Cr (VI) in aquatic environment}, series = {Sensors and actuators. B: Chemical}, volume = {Vol. 182}, journal = {Sensors and actuators. B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-3077 (E-Journal); 0925-4005 (Print)}, pages = {58 -- 65}, year = {2013}, language = {en} } @article{BohrnStuetzFleischeretal.2011, author = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {1345 -- 1350}, year = {2011}, language = {en} } @article{BohrnStuetzFleischeretal.2013, author = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Using a cell-based gas biosensor for investigation of adverse effects of acetone vapors in vitro}, series = {Biosensors and Bioelectronics. 40 (2013), H. 1}, journal = {Biosensors and Bioelectronics. 40 (2013), H. 1}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0956-5663}, pages = {393 -- 400}, year = {2013}, language = {en} } @inproceedings{BohrnStuetzFleischeretal.2012, author = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Living cell-based gas sensor system for the detection of acetone in air}, isbn = {978-3-9813484-2-2}, doi = {10.5162/IMCS2012/3.2.3}, pages = {269 -- 272}, year = {2012}, language = {en} } @article{BongaertsEsserLorbachetal.2011, author = {Bongaerts, Johannes and Esser, Simon and Lorbach, Volker and Al-Momani, L{\´o}ay and M{\"u}ller, Michael A. and Franke, Dirk and Grondal, Christoph and Kurutsch, Anja and Bujnicki, Robert and Takors, Ralf and Raeven, Leon and Wubbolts, Marcel and Bovenberg, Roel and Nieger, Martin and Sch{\"u}rmann, Melanie and Trachtmann, Natalie and Kozak, Stefan and Sprenger, Georg A. and M{\"u}ller, Michael}, title = {Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis}, series = {Angewandte Chemie International Edition}, volume = {Vol. 50}, journal = {Angewandte Chemie International Edition}, number = {Iss. 34}, publisher = {Wiley}, address = {Weinheim}, issn = {1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)}, pages = {7781 -- 7786}, year = {2011}, language = {en} } @article{BorgmeierBongaertsMeinhardt2012, author = {Borgmeier, Claudia and Bongaerts, Johannes and Meinhardt, Friedhelm}, title = {Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production}, series = {Journal of biotechnology}, volume = {159}, journal = {Journal of biotechnology}, number = {1-2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2012.02.011}, pages = {12 -- 20}, year = {2012}, abstract = {Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele - known to cause hypersecretion in Bacillus subtilis - resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG - known to interfere with DegU DNA-binding in B. subtilis - did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.}, language = {en} } @article{BrahmaMusioIsmayilovaetal.2015, author = {Brahma, Aischarya and Musio, Biagia and Ismayilova, Uliviya and Nikbin, Nikzad and Kamptmann, Sonja B. and Siegert, Petra and Jeromin, G{\"u}nter Erich and Ley, Steven and Pohl, Martina}, title = {An orthogonal biocatalytic approach for the safe generation and use of HCN in a multi-step continuous preparation of chiral O-acetylcyanohydrins}, series = {Synlett}, journal = {Synlett}, number = {Publ. online 29.09.2015}, publisher = {Thieme}, address = {Stuttgart}, issn = {0936-5214 (Print) ; 1437-2096 (e-Journal)}, doi = {10.1055/s-0035-1560644}, year = {2015}, language = {de} } @article{BresselSchultzeKhanetal.2003, author = {Bressel, A. and Schultze, J.W. and Khan, W. and Wolfaardt, G. M. and Rohns, H.-P. and Irmscher, R. and Sch{\"o}ning, Michael Josef}, title = {High resolution gravimetric, optical and electrochemical investigations of microbial biofilm formation in aqueous systems}, series = {Electrochimica Acta. 48 (2003), H. 20-22}, journal = {Electrochimica Acta. 48 (2003), H. 20-22}, isbn = {0013-4686}, pages = {3363 -- 3372}, year = {2003}, language = {en} } @article{BreuerMangSchoeningetal.2017, author = {Breuer, Lars and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, Ronald and Wagner, Torsten}, title = {Investigation of the spatial resolution of a laser-based stimulation process for light-addressable hydrogels with incorporated graphene oxide by means of IR thermography}, series = {Sensors and Actuators A: Physical}, volume = {268}, journal = {Sensors and Actuators A: Physical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0924-4247}, doi = {10.1016/j.sna.2017.11.031}, pages = {126 -- 132}, year = {2017}, language = {en} } @article{BreuerPilasGuthmannetal.2019, author = {Breuer, Lars and Pilas, Johanna and Guthmann, Eric and Sch{\"o}ning, Michael Josef and Thoelen, Ronald and Wagner, Torsten}, title = {Towards light-addressable flow control: responsive hydrogels with incorporated graphene oxide as laser-driven actuator structures within microfluidic channels}, series = {Sensor and Actuators B: Chemical}, volume = {288}, journal = {Sensor and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2019.02.086}, pages = {579 -- 585}, year = {2019}, language = {en} } @article{BreuerRaueKirschbaumetal.2015, author = {Breuer, Lars and Raue, Markus and Kirschbaum, M. and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, R. and Wagner, Torsten}, title = {Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431944}, pages = {1368 -- 1374}, year = {2015}, abstract = {Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.}, language = {en} }