@article{WuPoghossianBronderetal.2016, author = {Wu, Chunsheng and Poghossian, Arshak and Bronder, Thomas and Sch{\"o}ning, Michael Josef}, title = {Sensing of double-stranded DNA molecules by their intrinsic molecular charge using the light-addressable potentiometric sensor}, series = {Sensors and Actuators B: Chemical}, journal = {Sensors and Actuators B: Chemical}, number = {229}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2016.02.004}, pages = {506 -- 512}, year = {2016}, abstract = {A multi-spot light-addressable potentiometric sensor (LAPS), which belongs to the family of semiconductor field-effect devices, was applied for label-free detection of double-stranded deoxyribonucleic acid (dsDNA) molecules by their intrinsic molecular charge. To reduce the distance between the DNA charge and sensor surface and thus, to enhance the electrostatic coupling between the dsDNA molecules and the LAPS, the negatively charged dsDNA molecules were electrostatically adsorbed onto the gate surface of the LAPS covered with a positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)). The surface potential changes in each spot of the LAPS, induced by the layer-by-layer adsorption of a PAH/dsDNA bilayer, were recorded by means of photocurrent-voltage and constant-photocurrent measurements. In addition, the surface morphology of the gate surface before and after consecutive electrostatic adsorption of PAH and dsDNA layers was studied by atomic force microscopy measurements. Moreover, fluorescence microscopy was used to verify the successful adsorption of dsDNA molecules onto the PAH-modified LAPS surface. A high sensor signal of 25 mV was registered after adsorption of 10 nM dsDNA molecules. The lower detection limit is down to 0.1 nM dsDNA. The obtained results demonstrate that the PAH-modified LAPS device provides a convenient and rapid platform for the direct label-free electrical detection of in-solution hybridized dsDNA molecules.}, language = {en} } @article{WuBronderPoghossianetal.2015, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Werner, Frederik and Sch{\"o}ning, Michael Josef}, title = {Label-free detection of DNA using light-addressable potentiometric sensor modified with a positively charged polyelectrolyte layer}, series = {Nanoscale}, volume = {14}, journal = {Nanoscale}, number = {7}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, doi = {10.1039/C4NR07225A}, pages = {6143 -- 6150}, year = {2015}, abstract = {A multi-spot (16 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure modified with a weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. To achieve a preferentially flat orientation of DNA strands and thus, to reduce the distance between the DNA charge and MLAPS surface, the negatively charged probe single-stranded DNAs (ssDNA) were electrostatically adsorbed onto the positively charged PAH layer using a simple layer-by-layer (LbL) technique. In this way, more DNA charge can be positioned within the Debye length, yielding a higher sensor signal. The surface potential changes in each spot induced due to the surface modification steps (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), non-specific adsorption of mismatched ssDNA) were determined from the shifts of photocurrent-voltage curves along the voltage axis. A high sensor signal of 83 mV was registered after immobilization of probe ssDNA onto the PAH layer. The hybridization signal increases from 5 mV to 32 mV with increasing the concentration of cDNA from 0.1 nM to 5 μM. In contrast, a small signal of 5 mV was recorded in the case of non-specific adsorption of fully mismatched ssDNA (5 μM). The obtained results demonstrate the potential of the MLAPS in combination with the simple and rapid LbL immobilization technique as a promising platform for the future development of multi-spot light-addressable label-free DNA chips with direct electrical readout.}, language = {en} } @article{WuBronderPoghossianetal.2014, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Werner, Frederik and B{\"a}cker, Matthias and Sch{\"o}ning, Michael Josef}, title = {Label-free electrical detection of DNA with a multi-spot LAPS: First step towards light-addressable DNA chips}, series = {Physica status solidi A : Applications and materials science}, volume = {211}, journal = {Physica status solidi A : Applications and materials science}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X (E-Journal); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print)}, doi = {10.1002/pssa.201330442}, pages = {1423 -- 1428}, year = {2014}, abstract = {A multi-spot (4 × 4 spots) light-addressable potentiometric sensor (MLAPS) consisting of an Al-p-Si-SiO2 structure has been applied for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization by the intrinsic molecular charge for the first time. Single-stranded probe ssDNA molecules (20 bases) were covalently immobilized onto the silanized SiO2 gate surface. The unspecific adsorption of mismatch ssDNA on the MLAPS gate surface was blocked by bovine serum albumin molecules. To reduce the screening effect and to achieve a high sensor signal, the measurements were performed in a low ionic-strength solution. The photocurrent-voltage (I-V) curves were simultaneously recorded on all 16 spots after each surface functionalization step. Large shifts of I-V curves of 25 mV were registered after the DNA immobilization and hybridization event. In contrast, a small potential shift (∼5 mV) was observed in case of mismatch ssDNA, revealing good specificity of the sensor. The obtained results demonstrate the potential of the MLAPS as promising transducer platform for the multi-spot label-free electrical detection of DNA molecules by their intrinsic molecular charge.}, language = {en} } @article{Wrede1996, author = {Wrede, Oliver}, title = {Mnemotechnik in graphischen Benutzeroberfl{\"a}chen}, series = {Form / Form-Diskurs : Zeitschrift f{\"u}r Design und Theorie. 1 (1996), H. 2}, journal = {Form / Form-Diskurs : Zeitschrift f{\"u}r Design und Theorie. 1 (1996), H. 2}, year = {1996}, language = {en} } @article{Wrede2005, author = {Wrede, Oliver}, title = {Wer nicht h{\"o}ren will muss sehen : ein Pl{\"a}doyer f{\"u}r eine entwurfsbezogene Bildwissenschaft}, series = {Boxhorn. 12 (2005), H. Sehsturz}, journal = {Boxhorn. 12 (2005), H. Sehsturz}, pages = {24 -- 25}, year = {2005}, language = {de} } @article{Wrede2016, author = {Wrede, Oliver}, title = {Continuity in travel information}, series = {Information Design Journal}, volume = {22}, journal = {Information Design Journal}, number = {2}, publisher = {John Benjamins}, address = {Amsterdam}, issn = {0142-5471}, doi = {10.1075/idj.22.2.09wre}, pages = {172 -- 178}, year = {2016}, abstract = {This article discusses the contrast between the information transportation companies provide to travellers and that of their brand messaging. Companies' brand messaging often portrays the service they provide as pleasant, stress free and perfect. Customers and users of the service, on the other hand, often describe their experience of the service as a negative one. This article suggests that the brand value would be greater if transportation companies paid more attention to the users' experience when designing their information systems, particularly in worst case scenarios.}, language = {en} } @article{Wosnitza1987, author = {Wosnitza, Franz}, title = {Ein Testbarkeitsanalysemaß f{\"u}r den testfreundlichen Entwurf integrierter Schaltkreise auf Register-Transfer-Niveau}, series = {ITG-Fachberichte. 98 (1987)}, journal = {ITG-Fachberichte. 98 (1987)}, pages = {291 -- 295}, year = {1987}, language = {de} } @article{WollertVedralGrimmetal.2004, author = {Wollert, J{\"o}rg and Vedral, Andreas and Grimm, A. and Holtmann, M.}, title = {Bluetooth-Sicherheit : wie viel Pers{\"o}nlichkeit gebe ich preis?}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, volume = {Bd. 53}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, number = {Sonderh. M{\"a}rz}, issn = {0013-5658}, pages = {30 -- 35}, year = {2004}, language = {de} } @article{WollertVedral2002, author = {Wollert, J{\"o}rg and Vedral, Andreas}, title = {Einsatz von Open-Source-Software f{\"u}r Bluetooth-Evaluationsprototypen : Anwendungsempfehlungen und Einsatzbeispiele}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, volume = {Bd. 51}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, number = {H. 22}, issn = {0013-5658}, pages = {70 -- 73}, year = {2002}, language = {de} } @article{WollertLeidinger2010, author = {Wollert, J{\"o}rg and Leidinger, Rafael}, title = {Automation trifft Automotive : SPS in der Fahrzeugtechnik}, series = {Elektronik automotive : Magazin f{\"u}r Entwicklungen in der Kfz-Elektronik und Telematik}, journal = {Elektronik automotive : Magazin f{\"u}r Entwicklungen in der Kfz-Elektronik und Telematik}, number = {4/5}, publisher = {WEKA Fachmedien}, address = {Haar}, issn = {1614-0125}, pages = {44 -- 48}, year = {2010}, abstract = {Eine SPS im Kraftfahrzeug? Das geht ja gar nicht - da gibt es doch all die Hersteller f{\"u}r Kfz-Steuerger{\"a}te, die konfigurierbare Hardware anbieten. Im Prinzip ja, aber leider sind gerade die Sensoren oder Aktoren nicht ansteuerbar, die der Entwickler genau f{\"u}r sein aktuelles Projekt ben{\"o}tigt. Dieser Beitrag gibt einen kleinen Einblick in neue M{\"o}glichkeiten des Rapid Prototyping f{\"u}r mechatronische Systeme auf der Basis von Speicherprogrammierbaren Steuerungen (SPS).}, language = {de} } @article{WollertKruseVedral2007, author = {Wollert, J{\"o}rg and Kruse, Thomas and Vedral, Andreas}, title = {Die Kraft der drei Antennen : zuverl{\"a}ssige Funkkommunikation f{\"u}r die Automatisierungstechnik}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, volume = {56}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, number = {Sonderh. 2}, issn = {0013-5658; 1611-5120}, pages = {36 -- 42}, year = {2007}, language = {de} } @article{WollertFiedler1996, author = {Wollert, J{\"o}rg and Fiedler, J{\"o}rg}, title = {atp-Marktanalyse Echtzeitbetriebssysteme}, series = {Automatisierungstechnische Praxis : atp ; Praxis der Meß-, Steuerungs-, Regelungs- und Informationstechnik mit Softwaretechnik}, volume = {Bd. 38}, journal = {Automatisierungstechnische Praxis : atp ; Praxis der Meß-, Steuerungs-, Regelungs- und Informationstechnik mit Softwaretechnik}, number = {H. 1}, issn = {0340-4730}, pages = {33 -- 44}, year = {1996}, language = {de} } @article{WollertFiedlerStockmann1994, author = {Wollert, J{\"o}rg and Fiedler, J. and Stockmann, T.}, title = {Automatisieren mit dem PC : was der Personal Computer kann und wo er passen muss}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, number = {Nr. 6}, issn = {0013-5658}, pages = {76 -- 82}, year = {1994}, language = {de} } @article{WollertBuda2009, author = {Wollert, J{\"o}rg and Buda, Aurel}, title = {Funkl{\"o}sungen im parallelen Betrieb}, series = {Intelligenter produzieren : die Wissensplattform f{\"u}r das technische Management}, journal = {Intelligenter produzieren : die Wissensplattform f{\"u}r das technische Management}, number = {H. 4}, publisher = {VDMA-Verl.}, address = {Frankfurt, M.}, issn = {1869-2745}, pages = {6 -- 8}, year = {2009}, language = {de} } @article{WollertBooke2016, author = {Wollert, J{\"o}rg and Booke, Andreas}, title = {IoT von der Stange}, series = {elektronik}, journal = {elektronik}, number = {21 (2016)}, publisher = {WEKA-Fachmedien}, address = {M{\"u}nchen}, pages = {30 -- 37}, year = {2016}, abstract = {Heute sollte am besten jedes Ger{\"a}t in die große Rechnerwolke eingebettet werden. Doch so einfach ist das nicht, denn Cloud ist viel mehr als nur das Internet der Dinge. Als Anwender muss man sich also fragen, welche Dienste man m{\"o}chte und welchem Anbieter man sein Vertrauen schenkt.}, language = {de} } @article{Wollert2014, author = {Wollert, J{\"o}rg}, title = {Funkstandards f{\"u}r Sensornetzwerke}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, volume = {Bd. 63}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, number = {H. 12}, publisher = {WEKA-Fachmedien}, address = {Haar}, issn = {0013-5658}, pages = {36 -- 41}, year = {2014}, language = {de} } @article{Wollert2014, author = {Wollert, J{\"o}rg}, title = {Echtzeit-Ethernet : Protokoll-Reigen}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, volume = {Bd. 63}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, number = {H. 26}, publisher = {WEKA-Fachmedien}, address = {Haar}, issn = {0013-5658}, pages = {24 -- 31}, year = {2014}, language = {de} } @article{Wollert2014, author = {Wollert, J{\"o}rg}, title = {Der Embedded Wireless-Report}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, publisher = {WEKA-Fachmedien}, address = {Haar}, issn = {0013-5658}, pages = {18 -- 25}, year = {2014}, language = {de} } @article{Wollert2014, author = {Wollert, J{\"o}rg}, title = {Echtzeit-Ethernet mit Standardtechnik}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Embedded : Fachmagazin f{\"u}r die Entwicklung von Embedded-Systemen}, volume = {Bd. 63}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Embedded : Fachmagazin f{\"u}r die Entwicklung von Embedded-Systemen}, number = {H. 12}, publisher = {WEKA-Fachmedien}, address = {Haar}, issn = {0013-5658}, pages = {28 -- 32}, year = {2014}, language = {de} } @article{Wollert1998, author = {Wollert, J{\"o}rg}, title = {Im Sog des Standards : Windows CE als Betriebssystem f{\"u}r Embedded Controller}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, volume = {Bd. 47}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler}, number = {H. 9}, issn = {0013-5658}, pages = {40 -- 51}, year = {1998}, language = {de} }