@inproceedings{Ritz2001, author = {Ritz, Thomas}, title = {DISMED - an innovation portal for printing and media industry}, series = {CRIS 2000 : the fifth conference on Current Research Information Systems in Europe, in Helsinki (Finland), 25-27 May 2000}, booktitle = {CRIS 2000 : the fifth conference on Current Research Information Systems in Europe, in Helsinki (Finland), 25-27 May 2000}, publisher = {CORDIS Archive}, address = {[s.l.]}, pages = {9 S. : graph. Darst.}, year = {2001}, language = {en} } @article{DiltheyBrandenburgSchleser2004, author = {Dilthey, Ulrich and Brandenburg, Annette and Schleser, Markus}, title = {Dispensing and application of unfilled adhesives in the micro range}, series = {Welding and cutting. 3 (2004), H. 4}, journal = {Welding and cutting. 3 (2004), H. 4}, issn = {1612-3433}, pages = {250 -- 254}, year = {2004}, language = {en} } @inproceedings{ButenwegGellert2008, author = {Butenweg, Christoph and Gellert, Christoph}, title = {Displacement based design of masonry structures}, series = {Proceedings of the 14th International Brick and Block Masonry Conference : (Incorporating the 8th Australasian Masonry Conference) : Sydney, Australia, 13.-20. February 2008 / ed. Mark Masia ...}, booktitle = {Proceedings of the 14th International Brick and Block Masonry Conference : (Incorporating the 8th Australasian Masonry Conference) : Sydney, Australia, 13.-20. February 2008 / ed. Mark Masia ...}, publisher = {University of Newcastle}, address = {Callaghan}, organization = {International Brick and Block Masonry Conference <14, 2008, Sydney>}, isbn = {978-19-2070-1-92-5}, pages = {1 -- 10}, year = {2008}, language = {en} } @techreport{KestiBurstrandKnutssonSalvatoreetal.2008, author = {Kesti, J. and Burstrand Knutsson, H. and Salvatore, W. and Saouridis, C. and Odrieres Mer{\´e}, J. and Georgi, H. and Casajus, A. and Butz, C. and Schneider, R. and D{\"o}ring, Bernd}, title = {Dissemination of knowledge in the use of steel-intensive dry construction systems in housing : final report. Contract No RFS2-CT-2004-00036. EUR 22849 EN}, publisher = {Office for Official Publications of the European Communities}, address = {Luxembourg}, organization = {European Commission / Research Fund for Coal and Steel}, isbn = {978-92-79-05973-5}, pages = {205 S.}, year = {2008}, language = {en} } @article{SchwarzerKrings1996, author = {Schwarzer, Klemens and Krings, T.}, title = {Dissemination of solar cookers with and without temporary storage : a report of concrete examples ; experiences from Chile, India and Mali}, series = {Proceedings / EuroSun '96 : 10. Internationales Sonnenforum. Ed. by A. Goetzberger ... - Bd. 4}, journal = {Proceedings / EuroSun '96 : 10. Internationales Sonnenforum. Ed. by A. Goetzberger ... - Bd. 4}, publisher = {DGS-Sonnenenergie-Verl.}, address = {M{\"u}nchen}, pages = {1479 -- 1482}, year = {1996}, language = {en} } @article{BaumannLaraHubbertenetal.1997, author = {Baumann, Marcus and Lara, R.J. and Hubberten, D.N. and Thomas, D.N.}, title = {Dissolved organic matter studies in enclosed systems: Application of hydophobic fractionation for the assessment of organic nitrogen dynamics / Lara, R.J. ; Hubberten, U. ; Thomas, D.N. ; Baumann, M.E.M. ; Kattner, G.}, series = {Journal of Marine Systems. 13 (1997), H. 1-4}, journal = {Journal of Marine Systems. 13 (1997), H. 1-4}, isbn = {0924-7963}, pages = {155 -- 161}, year = {1997}, language = {en} } @article{WerkhausenCroninAlbrachtetal.2019, author = {Werkhausen, Amelie and Cronin, Neil J. and Albracht, Kirsten and Bojsen-M{\o}ller, Jens and Seynnes, Olivier R.}, title = {Distinct muscle-tendon interaction during running at different speeds and in different loading conditions}, series = {Journal of Applied Physiology}, volume = {127}, journal = {Journal of Applied Physiology}, number = {1}, issn = {1522-1601}, doi = {10.1152/japplphysiol.00710.2018}, pages = {246 -- 253}, year = {2019}, language = {en} } @article{BaumannTillmannAletsee1989, author = {Baumann, Marcus and Tillmann, Urban and Aletsee, Ludwig}, title = {Distribution of Carbon Among Photosynthetic End Products in the Bloom-Forming Arctic Diatom Thalassiosira antarctica COMBER / Tillmann, U. ; Baumann, M.E.M. ; Aletsee, L.}, series = {Polar Biology. 10 (1989), H. 3}, journal = {Polar Biology. 10 (1989), H. 3}, isbn = {0722-4060}, pages = {231 -- 238}, year = {1989}, language = {en} } @article{BaumannGradinger1991, author = {Baumann, Marcus and Gradinger, R.}, title = {Distribution of Phytoplankton Communities in Relation to the Large Scale Hydrographical Regime in the Fram Strait 1984 / Gradinger, R. ; Baumann, M.E.M.}, series = {Marine Biology. 111 (1991), H. 2}, journal = {Marine Biology. 111 (1991), H. 2}, isbn = {0025-3162}, pages = {311 -- 321}, year = {1991}, language = {en} } @incollection{SteuerDankertBouffierGaedickeetal.2017, author = {Steuer-Dankert, Linda and Bouffier, Anna and Gaedicke, Sonja and Leicht-Scholten, Carmen}, title = {Diversifying engineering education: a transdisciplinary approach from RWTH Aachen University}, series = {Strategies for increasing diversity in engineering majors and careers}, booktitle = {Strategies for increasing diversity in engineering majors and careers}, publisher = {IGI Global}, address = {Hershey, USA}, isbn = {9781522522126}, doi = {10.4018/978-1-5225-2212-6.ch010}, pages = {201 -- 235}, year = {2017}, abstract = {Engineers and therefore engineering education are challenged by the increasing complexity of questions to be answered globally. The education of future engineers therefore has to answer with curriculums that build up relevant skills. This chapter will give an example how to bring engineering and social responsibility successful together to build engineers of tomorrow. Through the integration of gender and diversity perspectives, engineering research and teaching is expanded with new perspectives and contents providing an important potential for innovation. Aiming on the enhancement of engineering education with distinctive competencies beyond technical expertise, the teaching approach introduced in the chapter represents key factors to ensure that coming generations of engineers will be able to meet the requirements and challenges a changing globalized world holds for them. The chapter will describe how this approach successfully has been implemented in the curriculum in engineering of a leading technical university in Germany.}, language = {en} } @article{SteuerDankertSharmaBlecketal.2017, author = {Steuer-Dankert, Linda and Sharma, Mamta Rameshwarlal and Bleck, Wolfgang and Leicht-Scholten, Carmen}, title = {Diversity and innovation management in large research groups}, series = {International Journal of Innovation Management}, volume = {5}, journal = {International Journal of Innovation Management}, number = {2}, issn = {1757-5877}, pages = {49 -- 72}, year = {2017}, abstract = {Contemporary research appreciates a diverse workforce as a potential source of innovation. Researchers explore the fine details of why diversity management is central for generating innovations in heterogeneous research groups and how it could be effectively implemented into organizations. Complex research associations that discuss topics with a high impact on society increasingly address the necessity of establishing a diverse workforce to confront the challenges of tomorrow. Characterized by complex management structures as well as hierarchies, research associations have not been a subject of investigation until now. For this reason, the presented research project aims to develop a diversity and innovation management strategy with the ultimate goal of inducing change in the corporate culture. The proposed approach consisted of six phases; the first two phases investigated the status quo of diversity in the existing organizational structures of member institutes and the variety of particular working cultures within the research association. The third and the fourth phases utilized qualitative and quantitative studies. The third phase focused on the connection of management level to diversity and innovation, and the need for diversity and innovation management, and tailor-made methods of implementing them. The first three phases have been accomplished successfully; preliminary results are already available. The fourth phase will mainly focus on exploring the mind-set of the employees. The fifth phase will consolidate the findings in the first four phases into an implementable strategy. The final phase will address the implementation of this strategy into the organization. Phases 4 to 6 have not yet been undertaken}, language = {en} } @phdthesis{SteuerDankert2020, author = {Steuer-Dankert, Linda}, title = {Diversity in complex organizations : the triangle of diversity management, change management and organizational culture from a system-theoretical perspective}, doi = {10.18154/RWTH-2020-11830}, pages = {298}, year = {2020}, abstract = {Insbesondere im wirtschaftlichen Kontext wird die Diversit{\"a}t von Belegschaften zunehmend als ein kritischer Erfolgsfaktor gesehen. Neben dem Potenzial, welches sich laut Studien aus einem vielf{\"a}ltigen Team ergibt, werden jedoch ebenfalls die aus menschlicher Diversit{\"a}t resultierenden Herausforderungen thematisiert und wissenschaftlich untersucht. Sowohl aus dem Potenzial als auch aus den Herausforderungen ergibt sich dabei die Notwendigkeit der Implementierung eines organisationsspezifischen Diversity Managements, welches die Gewinnung neuer Mitarbeiter*innen einerseits und das Management der vorhandenen Vielfalt andererseits gleichermaßen unterst{\"u}tzt. In der psychologischen, sozial- und wirtschaftswissenschaftlichen Literatur gibt es unterschiedliche Definitionen von Diversit{\"a}t, woraus sich verschiedene Perspektiven auf das Vorgehen bei der Gestaltung und Umsetzung eines Diversity Management Ansatzes ergeben. Insbesondere vor dem Hintergrund der Komplexit{\"a}t des Organisationsumfeldes und der steigenden Anforderungen an die organisationsinterne Agilit{\"a}t besteht die Notwendigkeit, Diversit{\"a}t in Organisationen st{\"a}rker zu reflektieren und systemspezifische Ans{\"a}tze zu entwickeln. Dies erfordert die Ber{\"u}cksichtigung organisationsspezifischer Strukturen und Prozesse sowie die Reflexion des Wandels der Organisationskultur durch die Umsetzung eines Diversity Management Ansatzes, der die gegebene Komplexit{\"a}t aufgreift und bew{\"a}ltigen kann. Dar{\"u}ber hinaus sind die psychologischen Auswirkungen solcher Ver{\"a}nderungen auf die Mitarbeiter*innen zu ber{\"u}cksichtigen, um Reaktanzen zu vermeiden und eine nachhaltige Umsetzung von Diversity Management zu erm{\"o}glichen. In Ermangelung entsprechender Ans{\"a}tze im Rahmen {\"o}ffentlich finanzierter, komplexer Forschungsorganisationen, ist das Ziel dieser Dissertation die Entwicklung und Erprobung eines Forschungsdesigns, welches die Ans{\"a}tze des Diversity- und Change Managements mit der Organisationskultur verkn{\"u}pft, indem es eine systemtheoretische Perspektive einnimmt. Dabei wird das Forschungsdesign auf eine komplexe wissenschaftliche Organisation angewendet. Als Basis dient die in Teil A durchgef{\"u}hrte Betrachtung des aktuellen Forschungsstandes aus einer interdisziplin{\"a}ren Perspektive und die damit einhergehende umfassende Einf{\"u}hrung in das Forschungsfeld. Im Zuge dessen wird detailliert auf die begriffliche Definition von Diversit{\"a}t eingegangen, bevor dann die psychologischen Konzepte im Diversit{\"a}tskontext den {\"U}bergang zu einer differenzierten Auseinandersetzung mit dem Konzept des Diversity Managements bilden. Auf dieser Grundlage werden das Forschungsdesign sowie die daraus resultierenden Forschungsphasen abgeleitet. Teil A stellt somit die theoretische Grundlage f{\"u}r die in Teil B pr{\"a}sentierten Fachaufs{\"a}tze dar. Jeder Fachaufsatz beleuchtet dabei in chronologischer Reihenfolge die unterschiedlichen Forschungsphasen. Fachaufsatz I pr{\"a}sentiert den sechsstufigen Forschungsansatz und beleuchtet die besonderen Rahmenbedingungen des Forschungsobjektes aus einer theoretischen Perspektive. Im Anschluss werden die Ergebnisse der Organisationsanalyse, welche zugleich Phase I und II des Forschungskonzeptes darstellen, vorgestellt. Aufbauend auf diesen Forschungsergebnissen fokussiert Forschungsaufsatz II die Darlegung der Ergebnisse aus Forschungsphase III, der Befragung der F{\"u}hrungsebene. Die Befragung thematisierte dabei die Wahrnehmung von Diversity und Diversity Management auf F{\"u}hrungsebene, die Verkn{\"u}pfung von Diversit{\"a}t mit Innovation sowie die Reflexion des eigenen F{\"u}hrungsstils. Als Ergebnis der Befragung konnten sechs Typen identifiziert werden, die das F{\"u}hrungsverst{\"a}ndnis im Diversit{\"a}tskontext widerspiegeln und somit den Ansatzpunkt f{\"u}r eine top-down gerichtete Diversity Management Strategie darstellen. Darauf aufbauend wird in Forschungsphase IV die Mitarbeiter*innenebene beforscht. Im Zentrum der quantitativen Befragung standen die vorherrschenden Einstellungen zum Themenkomplex Diversity und Diversity Management, die Wahrnehmung von Diversit{\"a}t sowie die Untersuchung des Einflusses der F{\"u}hrungsebene auf die Mitarbeiter*innenebene. Forschungsaufsatz III pr{\"a}sentiert erste Ergebnisse dieser Untersuchung. Die Analyse weist auf eine unterschiedliche Gewichtung der verschiedenen Diversit{\"a}tskategorien hinsichtlich der Verkn{\"u}pfung mit Innovationen und somit der Reflexion des Kontextes zwischen Diversit{\"a}t und Innovationen hin. Vergleichbar mit den identifizierten Typen auf der F{\"u}hrungsebene, deutet die Analyse auf die Existenz unterschiedlicher Reflexionsgrade auf Mitarbeiter*innenebene hin. Auf Basis dessen wird im Rahmen von Forschungsaufsatz IV eine n{\"a}here Untersuchung des Reflexionsgrades auf Mitarbeiter*innenebene pr{\"a}sentiert und der Diversity Management Ansatz mit Elementen des Change Managements kombiniert. Besondere Ber{\"u}cksichtigung findet als Schlussfolgerung einer theoretischen Analyse die Organisationskultur als zentrales Element bei der Entwicklung und Einf{\"u}hrung eines Diversity Management Ansatzes in eine komplexe Forschungsorganisation in Deutschland. Die Analyse zeigt, dass die Wahrnehmung von Diversit{\"a}t heterogen aber zun{\"a}chst losgel{\"o}st vom individuellen Hintergrund ist (im Rahmen dieser Analyse lag der Fokus auf den Diversit{\"a}tskategorien Gender und Herkunft). Hinsichtlich der Wertsch{\"a}tzung von Diversit{\"a}t zeigt sich dabei ebenfalls ein heterogenes Bild. In der Gesamtbetrachtung stimmen lediglich 17\% der Mitarbeiter*innen zu, dass Diversit{\"a}tskategorien wie Gender, Herkunft oder auch Alter einen Mehrwert darstellen k{\"o}nnen. Zugleich bewertet diese Gruppe die dem Thema beigemessene Wichtigkeit im CoE als ausreichend. Zusammengefasst lassen sich folgende Erkenntnisse im Rahmen dieser Dissertation ableiten und dienen somit als Grundlage f{\"u}r die Entwicklung eines Diversity Management Ansatzes: (1) Die Entwicklung eines bedarfsorientierten Diversity Management Ansatzes erfordert einen systemtheoretischen Prozess, der sowohl organisationsinterne als auch externe Einflussfaktoren ber{\"u}cksichtigt. Der im Rahmen des Forschungsprojektes entwickelte sechsstufige Forschungsprozess hat sich dabei als geeignetes Instrument erwiesen. (2)Im Rahmen {\"o}ffentlicher Forschungseinrichtungen lassen sich dabei drei zentrale Faktoren identifizieren: die individuelle Reflexionsebene, die Organisationskultur sowie extern beeinflusste Organisationsstrukturen, Prozesse und Systeme.(3)Vergleichbar mit privatwirtschaftlichen Unternehmen hat auch in wissenschaftlichen Organisationen die F{\"u}hrungsebene einen maßgeblichen Einfluss auf die Wahrnehmung von Diversit{\"a}t und somit einen Einfluss auf die Umsetzung einer Diversity Management Strategie. Daher ist auch im wissenschaftlichen Kontext, bedingt durch die rechtlichen Rahmenbedingungen des Hochschulsystems, ein top-down Ansatz f{\"u}r eine nachhaltige Implementierung erforderlich. (4) Diversity Management steht in einem engen Zusammenhang mit einem organisationalen Wandel, was die Reflexion von Ver{\"a}nderungsprozesse aus einer psychologischen Perspektive erfordert und eine Verkn{\"u}pfung von Diversity und Change Management bedingt. Aufbauend auf den im Rahmen des entwickelten Forschungskonzeptes gewonnenen zentralen Erkenntnissen wird ein Ansatz entwickelt, der die Ableitung theoretischer Implikationen sowie Implikationen f{\"u}r das Management erm{\"o}glicht. Insbesondere vor dem Hintergrund der Reflexion der besonderen Rahmenbedingungen {\"o}ffentlich finanzierter Forschungsorganisationen werden dar{\"u}ber hinaus politische Implikationen abgeleitet, die auf die Ver{\"a}nderung struktureller Dimensionen abzielen.}, language = {en} } @inproceedings{SteuerDankertLeichtScholten2019, author = {Steuer-Dankert, Linda and Leicht-Scholten, Carmen}, title = {Diversity- and innovation management in complex engineering organizations}, series = {7th International OFEL Conference on Governance, Management and Entrepreneurship: Embracing Diversity in Organisations. April 5th - 6th, 2019, Dubrovnik, Croatia}, booktitle = {7th International OFEL Conference on Governance, Management and Entrepreneurship: Embracing Diversity in Organisations. April 5th - 6th, 2019, Dubrovnik, Croatia}, editor = {Tipurić, Darko and Hruška, Domagoj}, publisher = {Governance Research and Development Centre (CIRU)}, address = {Zagreb}, pages = {136 -- 157}, year = {2019}, abstract = {Diversity is increasingly being addressed as an innovation-promoting factor. For this reason, companies and institutions tackle the integration of a diversity management approach that enables a heterogenic perspective on innovation development. However, system-theoretical frameworks state that the implementation of diversity measures that are not tailored to the needs of the organization often leads to a rejection or reactivity with regard to the management approach. In this context, especially organizations, which are characterized by a specific hierarchical structure, a dominant habitus or specialist culture, must face the challenge of realizing a sustainable change of the corporate culture that sets the basis for implementing diversity management approaches. The presented research project focuses on analyzing the situation in a huge scientific collaborative project - so called Cluster of Excellence (CoE) - with the aim to implement a diversity - and innovation management strategy. Considering the influencing determinants, the CoE is characterized by its embeddedness in the scientific system, a complex organizational structure, and a high fluctuation rate. The paper presents a systemic approach of reflecting these factors in order to develop a diversity- and innovation management strategy. In this frame, the results of a quantitative survey of CoE employees and derived mindset-types are presented. The results show a need for taking different mindset-types into account, to be able to develop a tailored management strategy. The aim of the project is to give recommendations for developing a sustainable management concept that promotes both diversity and innovation by drawing on the persisting mindsets of organization members while reflecting top down as well as bottom up factors of implementation processes as well as the psychology of change. This paper addresses all who are concerned with the management of human resources in innovation processes and are striving for a cultural change within the framework of complex organizations.}, language = {en} } @article{BongaertsEsserLorbachetal.2011, author = {Bongaerts, Johannes and Esser, Simon and Lorbach, Volker and Al-Momani, L{\´o}ay and M{\"u}ller, Michael A. and Franke, Dirk and Grondal, Christoph and Kurutsch, Anja and Bujnicki, Robert and Takors, Ralf and Raeven, Leon and Wubbolts, Marcel and Bovenberg, Roel and Nieger, Martin and Sch{\"u}rmann, Melanie and Trachtmann, Natalie and Kozak, Stefan and Sprenger, Georg A. and M{\"u}ller, Michael}, title = {Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis}, series = {Angewandte Chemie International Edition}, volume = {Vol. 50}, journal = {Angewandte Chemie International Edition}, number = {Iss. 34}, publisher = {Wiley}, address = {Weinheim}, issn = {1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)}, pages = {7781 -- 7786}, year = {2011}, language = {en} } @article{Bernecker2014, author = {Bernecker, Andreas}, title = {Divided Government and the Adoption of Economic Reforms}, series = {CESifo DICE Report - Journal for Institutional Comparison}, volume = {12}, journal = {CESifo DICE Report - Journal for Institutional Comparison}, number = {4}, publisher = {Ifo Institute for Economic Research}, address = {M{\"u}nchen}, issn = {1612-0663}, pages = {47 -- 52}, year = {2014}, language = {en} } @article{Bernecker2016, author = {Bernecker, Andreas}, title = {Divided we reform? Evidence from US welfare policies}, series = {Journal of Public Economics}, volume = {142}, journal = {Journal of Public Economics}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0047-2727}, doi = {10.1016/j.jpubeco.2016.08.003}, pages = {24 -- 38}, year = {2016}, abstract = {Divided government is often thought of as causing legislative deadlock. I investigate the link between divided government and economic reforms using a novel data set on welfare reforms in US states between 1978 and 2010. Panel data regressions show that, under divided government, a US state is around 25\% more likely to adopt a welfare reform than under unified government. Several robustness checks confirm this counter-intuitive finding. Case study evidence suggests an explanation based on policy competition between governor, senate, and house.}, language = {en} } @article{SeiblerSchuebelerFieringetal.1998, author = {Seibler, Jost and Sch{\"u}beler, Dirk and Fiering, Steven and Groudine, Mark and Bode, J{\"u}rgen}, title = {DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs}, series = {Biochemistry}, volume = {37}, journal = {Biochemistry}, number = {18}, issn = {1520-4995}, doi = {10.1021/bi980288t}, pages = {6229 -- 6234}, year = {1998}, language = {en} } @article{GamellaZakharchenkoGuzetal.2017, author = {Gamella, Maria and Zakharchenko, Andrey and Guz, Nataliia and Masi, Madeline and Minko, Sergiy and Kolpashchikov, Dmitry M. and Iken, Heiko and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Katz, Evgeny}, title = {DNA computing system activated by electrochemically triggered DNA realease from a polymer-brush-modified electrode array}, series = {Electroanalysis}, volume = {29}, journal = {Electroanalysis}, number = {2}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-4109}, doi = {10.1002/elan.201600389}, pages = {398 -- 408}, year = {2017}, abstract = {An array of four independently wired indium tin oxide (ITO) electrodes was used for electrochemically stimulated DNA release and activation of DNA-based Identity, AND and XOR logic gates. Single-stranded DNA molecules were loaded on the mixed poly(N,N-dimethylaminoethyl methacrylate) (PDMAEMA)/poly(methacrylic acid) (PMAA) brush covalently attached to the ITO electrodes. The DNA deposition was performed at pH 5.0 when the polymer brush is positively charged due to protonation of tertiary amino groups in PDMAEMA, thus resulting in electrostatic attraction of the negatively charged DNA. By applying electrolysis at -1.0 V(vs. Ag/AgCl reference) electrochemical oxygen reduction resulted in the consumption of hydrogen ions and local pH increase near the electrode surface. The process resulted in recharging the polymer brush to the negative state due to dissociation of carboxylic groups of PMAA, thus repulsing the negatively charged DNA and releasing it from the electrode surface. The DNA release was performed in various combinations from different electrodes in the array assembly. The released DNA operated as input signals for activation of the Boolean logic gates. The developed system represents a step forward in DNA computing, combining for the first time DNA chemical processes with electronic input signals.}, language = {en} } @article{BronderPoghossianSchejaetal.2015, author = {Bronder, Thomas and Poghossian, Arshak and Scheja, Sabrina and Wu, Chunsheng and Keusgen, Michael and Mewes, Dieter and Sch{\"o}ning, Michael Josef}, title = {DNA Immobilization and Hybridization Detection by the Intrinsic Molecular Charge Using Capacitive Field-Effect Sensors Modified with a Charged Weak Polyelectrolyte Layer}, series = {Applied Materials \& Interfaces}, volume = {36}, journal = {Applied Materials \& Interfaces}, number = {7}, publisher = {American Chemical Society}, address = {Washington, DC}, doi = {10.1021/acsami.5b05146}, pages = {20068 -- 20075}, year = {2015}, abstract = {Miniaturized setup, compatibility with advanced micro- and nanotechnologies, and ability to detect biomolecules by their intrinsic molecular charge favor the semiconductor field-effect platform as one of the most attractive approaches for the development of label-free DNA chips. In this work, a capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor covered with a layer-by-layer prepared, positively charged weak polyelectrolyte layer of PAH (poly(allylamine hydrochloride)) was used for the label-free electrical detection of DNA (deoxyribonucleic acid) immobilization and hybridization. The negatively charged probe single-stranded DNA (ssDNA) molecules were electrostatically adsorbed onto the positively charged PAH layer, resulting in a preferentially flat orientation of the ssDNA molecules within the Debye length, thus yielding a reduced charge-screening effect and a higher sensor signal. Each sensor-surface modification step (PAH adsorption, probe ssDNA immobilization, hybridization with complementary target DNA (cDNA), reducing an unspecific adsorption by a blocking agent, incubation with noncomplementary DNA (ncDNA) solution) was monitored by means of capacitance-voltage and constant-capacitance measurements. In addition, the surface morphology of the PAH layer was studied by atomic force microscopy and contact-angle measurements. High hybridization signals of 34 and 43 mV were recorded in low-ionic strength solutions of 10 and 1 mM, respectively. In contrast, a small signal of 4 mV was recorded in the case of unspecific adsorption of fully mismatched ncDNA. The density of probe ssDNA and dsDNA molecules as well as the hybridization efficiency was estimated using the experimentally measured DNA immobilization and hybridization signals and a simplified double-layer capacitor model. The results of field-effect experiments were supported by fluorescence measurements, verifying the DNA-immobilization and hybridization event.}, language = {en} } @inproceedings{WuBronderPoghossianetal.2014, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer}, series = {Sensoren und Messsysteme 2014 ; Beitr{\"a}ge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in N{\"u}rnberg. (ITG-Fachbericht ; 250)}, booktitle = {Sensoren und Messsysteme 2014 ; Beitr{\"a}ge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in N{\"u}rnberg. (ITG-Fachbericht ; 250)}, publisher = {VDE-Verl.}, address = {D{\"u}sseldorf}, organization = {VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, isbn = {978-3-8007-3622-5}, pages = {1 -- 4}, year = {2014}, language = {en} }