@article{HaegerWirgesTanzmannetal.2023, author = {Haeger, Gerrit and Wirges, Jessika and Tanzmann, Nicole and Oyen, Sven and Jolmes, Tristan and Jaeger, Karl-Erich and Sch{\"o}rken, Ulrich and Bongaerts, Johannes and Siegert, Petra}, title = {Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis}, series = {Microbial Cell Factories}, journal = {Microbial Cell Factories}, number = {22}, publisher = {Springer Nature}, issn = {1475-2859}, doi = {10.1186/s12934-023-02079-1}, pages = {Article number: 77 (2023)}, year = {2023}, abstract = {Background Aminoacylases are highly promising enzymes for the green synthesis of acyl-amino acids, potentially replacing the environmentally harmful Schotten-Baumann reaction. Long-chain acyl-amino acids can serve as strong surfactants and emulsifiers, with application in cosmetic industries. Heterologous expression of these enzymes, however, is often hampered, limiting their use in industrial processes. Results We identified a novel mycobacterial aminoacylase gene from Mycolicibacterium smegmatis MKD 8, cloned and expressed it in Escherichia coli and Vibrio natriegens using the T7 overexpression system. The recombinant enzyme was prone to aggregate as inclusion bodies, and while V. natriegens Vmax™ could produce soluble aminoacylase upon induction with isopropyl β-d-1-thiogalactopyranoside (IPTG), E. coli BL21 (DE3) needed autoinduction with lactose to produce soluble recombinant protein. We successfully conducted a chaperone co-expression study in both organisms to further enhance aminoacylase production and found that overexpression of chaperones GroEL/S enhanced aminoacylase activity in the cell-free extract 1.8-fold in V. natriegens and E. coli. Eventually, E. coli ArcticExpress™ (DE3), which co-expresses cold-adapted chaperonins Cpn60/10 from Oleispira antarctica, cultivated at 12 °C, rendered the most suitable expression system for this aminoacylase and exhibited twice the aminoacylase activity in the cell-free extract compared to E. coli BL21 (DE3) with GroEL/S co-expression at 20 °C. The purified aminoacylase was characterized based on hydrolytic activities, being most stable and active at pH 7.0, with a maximum activity at 70 °C, and stability at 40 °C and pH 7.0 for 5 days. The aminoacylase strongly prefers short-chain acyl-amino acids with smaller, hydrophobic amino acid residues. Several long-chain amino acids were fairly accepted in hydrolysis as well, especially N-lauroyl-L-methionine. To initially evaluate the relevance of this aminoacylase for the synthesis of N-acyl-amino acids, we demonstrated that lauroyl-methionine can be synthesized from lauric acid and methionine in an aqueous system. Conclusion Our results suggest that the recombinant enzyme is well suited for synthesis reactions and will thus be further investigated.}, language = {en} } @article{Hanrath1989, author = {Hanrath, Wilhelm}, title = {Character tables of some factor groups of space groups}, series = {Perfect groups / Derek F. Holt and W. Plesken}, journal = {Perfect groups / Derek F. Holt and W. Plesken}, publisher = {Clarendon Press}, address = {Oxford}, isbn = {0-19-853559-7}, pages = {357 -- 359}, year = {1989}, language = {en} } @article{SchwarzerSilva2008, author = {Schwarzer, Klemens and Silva, Maria Eugenia Vieira da}, title = {Characterisation and design methods of solar cookers / Schwarzer, Klemens ; Silva, Maria Eugenia Vieira da}, series = {Solar energy. 82 (2008), H. 2}, journal = {Solar energy. 82 (2008), H. 2}, isbn = {0038-092X}, pages = {157 -- 163}, year = {2008}, language = {en} } @inproceedings{ReisertGeisslerFloerkeetal.2012, author = {Reisert, Steffen and Geissler, H. and Fl{\"o}rke, R. and Weiler, C. and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Characterisation of aseptic sterilisation processes using an electronic nose}, series = {Nanoscale Science and Technology (NS\&T´12) : Proceedings Book Humboldt Kolleg ; Tunisia, 17-19 March, 2012}, booktitle = {Nanoscale Science and Technology (NS\&T´12) : Proceedings Book Humboldt Kolleg ; Tunisia, 17-19 March, 2012}, editor = {Abdelghani, Adnane and Sch{\"o}ning, Michael Josef}, pages = {45 -- 45}, year = {2012}, language = {en} } @article{ReisertGeisslerFloerkeetal.2013, author = {Reisert, Steffen and Geissler, Hanno and Fl{\"o}rke, Rudolf and Weiler, Christian and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Characterisation of aseptic sterilisation processes using an electronic nose}, series = {International journal of nanotechnology}, volume = {Vol. 10}, journal = {International journal of nanotechnology}, number = {No. 5-7}, publisher = {Inderscience Enterprises}, address = {Gen{\`e}ve}, issn = {1475-7435 (Print) 7141-8151 (Online)}, pages = {470 -- 484}, year = {2013}, language = {en} } @article{AbouzarPoghossianRazavietal.2009, author = {Abouzar, Maryam H. and Poghossian, Arshak and Razavi, A. and Williams, O. A. and Bijnens, N. and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Characterisation of capacitive field-effect sensors with a nanocrystalline-diamond film as transducer material for multi-parameter sensing}, series = {Biosensors and Bioelectronics. 24 (2009), H. 5}, journal = {Biosensors and Bioelectronics. 24 (2009), H. 5}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0956-5663}, pages = {1298 -- 1304}, year = {2009}, language = {en} } @article{KirchnerReisertPuetzetal.2012, author = {Kirchner, Patrick and Reisert, Steffen and P{\"u}tz, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Characterisation of polymeric materials as passivation layer for calorimetric H2O2 gas sensors}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100773}, pages = {859 -- 863}, year = {2012}, abstract = {Calorimetric gas sensors for monitoring the H₂O₂ concentration at elevated temperatures in industrial sterilisation processes have been presented in previous works. These sensors are built up in form of a differential set-up of a catalytically active and passive temperature-sensitive structure. Although, various types of catalytically active dispersions have been studied, the passivation layer has to be established and therefore, chemically as well as physically characterised. In the present work, fluorinated ethylene propylene (FEP), perfluoralkoxy (PFA) and epoxy-based SU-8 photoresist as temperature-stable polymeric materials have been investigated for sensor passivation in terms of their chemical inertness against H₂O₂, their hygroscopic properties as well as their morphology. The polymeric materials were deposited via spin-coating on the temperature-sensitive structure, wherein spin-coated FEP and PFA show slight agglomerates. However, they possess a low absorption of humidity due to their hydrophobic surface, whereas the SU-8 layer has a closed surface but shows a slightly higher absorption of water. All of them were inert against gaseous H₂O₂ during the characterisation in H₂O₂ atmosphere that demonstrates their suitability as passivation layer for calorimetric H₂O₂ gas sensors.}, language = {en} } @article{SchrothLuethHummeletal.2001, author = {Schroth, P. and L{\"u}th, H. and Hummel, Hans E. and Sch{\"u}tz, S. and Sch{\"o}ning, Michael Josef}, title = {Characterising an insect antenna as a receptor for a biosensor by means of impedance spectroscopy}, series = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, journal = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, publisher = {Elsevier [u.a.]}, address = {Amsterdam [u.a.]}, isbn = {0-08-044014-2}, pages = {293 -- 297}, year = {2001}, language = {en} } @book{GottschalkJanzRennings1998, author = {Gottschalk, Sandra and Janz, Norbert and Rennings, Klaus}, title = {Characteristics and Performance of Eco-Innovative Firms in the German Service Sector}, pages = {mimeo}, year = {1998}, language = {en} } @article{Lassonczyk1990, author = {Lassonczyk, Beate}, title = {Characteristics of a fossil soil developed on the basement complex in the northeast Somalia}, series = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, journal = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, isbn = {0172-8784}, pages = {713 -- 718}, year = {1990}, language = {en} } @article{HelsperMoelter1991, author = {Helsper, Christoph and M{\"o}lter, W.}, title = {Characteristics of a Nebulizer with Cyclone Preseparator / M{\"o}lter, W. ; Helsper, C.}, series = {Aerosols, their generation, behaviour and applications. Annual Conference of the Aerosol Society / Aerosol Society (UK) ; 5.}, journal = {Aerosols, their generation, behaviour and applications. Annual Conference of the Aerosol Society / Aerosol Society (UK) ; 5.}, address = {Loughborough}, pages = {89 -- 94}, year = {1991}, language = {en} } @inproceedings{OertelBung2012, author = {Oertel, Mario and Bung, Daniel B.}, title = {Characteristics of cross-bar block ramp flows}, series = {Hydraulic structures into the 21st century : 4th IAHR International Symposium on Hydraulic Structures : 9.-11.2.2012, Porto}, booktitle = {Hydraulic structures into the 21st century : 4th IAHR International Symposium on Hydraulic Structures : 9.-11.2.2012, Porto}, organization = {International Symposium on Hydraulic Structures <4, 2012, Porto>}, isbn = {978-989-8509-01-7}, pages = {Elektronisch publiziert}, year = {2012}, language = {en} } @inproceedings{RothMoehringTippkoetter2016, author = {Roth, J. and M{\"o}hring, S. and Tippk{\"o}tter, Nils}, title = {Characterization and evaluation of lignocellulosic biomass 130 hydrolysates for ABE fermentation}, series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany}, booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany}, publisher = {DECHEMA}, address = {Frankfurt am Main}, pages = {130}, year = {2016}, language = {en} } @article{MukherjeePrahlBlecketal.2010, author = {Mukherjee, Krishnendu and Prahl, Ulrich and Bleck, Wolfgang and Reisgen, Uwe and Schleser, Markus and Abdurakhmanov, Aydemir}, title = {Characterization and modelling techniques for gas metal arc welding of DP 600 sheet steels}, series = {Materialwissenschaft und Werkstofftechnik}, volume = {41}, journal = {Materialwissenschaft und Werkstofftechnik}, number = {11}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-4052}, doi = {10.1002/mawe.201000692}, pages = {972 -- 983}, year = {2010}, abstract = {The objectives of the present work are to characterize the Gas Metal Arc Welding process of DP 600 sheet steel and to summarize the modelling techniques. The time-temperature evolution during the welding cycle was measured experimentally and modelled with the softwaretool SimWeld. To model the phase transformations during the welding cycle dilatometer tests were done to quantify the parameters for phase field modelling by MICRESS®. The important input parameters are interface mobility, nucleation density, etc. A contribution was made to include austenite to bainite transformation in MICRESS®. This is useful to predict the microstructure in the fast cooling segments. The phase transformation model is capable to predict the microstructure along the heating and cooling cycles of welding. Tensile tests have shown the evidence of failure at the heat affected zone, which has the ferrite-tempered martensite microstructure.}, language = {en} } @article{HoltrupSadeghfamHeuermannetal.2014, author = {Holtrup, S. and Sadeghfam, Arash and Heuermann, Holger and Awakowicz, P.}, title = {Characterization and optimization technique for microwave-driven high-intensity discharge lamps using hot S-parameters}, series = {IEEE transactions on microwave theories and techniques}, volume = {62}, journal = {IEEE transactions on microwave theories and techniques}, number = {10}, publisher = {IEEE}, address = {New York}, issn = {0018-9480}, doi = {10.1109/TMTT.2014.2342652}, pages = {2471 -- 2480}, year = {2014}, abstract = {High-intensity discharge lamps can be driven by radio-frequency signals in the ISM frequency band at 2.45 GHz, using a matching network to transform the impedance of the plasma to the source impedance. To achieve an optimal operating condition, a good characterization of the lamp in terms of radio frequency equivalent circuits under operating conditions is necessary, enabling the design of an efficient matching network. This paper presents the characterization technique for such lamps and presents the design of the required matching network. For the characterization, a high-intensity discharge lamp was driven by a monofrequent large signal at 2.45 GHz, whereas a frequency sweep over 300 MHz was performed across this signal to measure so-called small-signal hot S-parameters using a vector network analyzer. These parameters are then used as an equivalent load in a circuit simulator to design an appropriate matching network. Using the measured data as a black-box model in the simulation results in a quick and efficient method to simulate and design efficient matching networks in spite of the complex plasma behavior. Furthermore, photometric analysis of high-intensity discharge lamps are carried out, comparing microwave operation to conventional operation.}, language = {en} } @article{SelmerKimDarleyetal.2006, author = {Selmer, Thorsten and Kim, Jihoe and Darley, Daniel and Buckel, Wolfgang}, title = {Characterization of (R)-2-hydroxyisocaproate dehydrogenase and a family III coenzyme A transferase involved in reduction of L-leucine to isocaproate by Clostridium difficile / Kim, J. ; Darley, D. ; Selmer, T. ; Buckel, W.}, series = {Applied and Environmental Microbiology. 72 (2006), H. 9}, journal = {Applied and Environmental Microbiology. 72 (2006), H. 9}, isbn = {0099-2240}, pages = {6062 -- 6069}, year = {2006}, language = {en} } @article{JossekBongaertsSprenger2001, author = {Jossek, Ralf and Bongaerts, Johannes and Sprenger, Georg A.}, title = {Characterization of a new feedback-resistant 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase AroF of Escherichia coli}, series = {FEMS microbiology letters}, volume = {Vol. 202}, journal = {FEMS microbiology letters}, number = {Iss. 1}, issn = {1574-6968}, pages = {145 -- 148}, year = {2001}, language = {en} } @article{SchusserPoghossianBaeckeretal.2012, author = {Schusser, Sebastian and Poghossian, Arshak and B{\"a}cker, Matthias and Leinhos, Marcel and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Characterization of biodegradable polymers with capacitive field-effect sensors}, series = {Sensors and actuators B: Chemical}, volume = {187}, journal = {Sensors and actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2012.07.099}, pages = {2 -- 7}, year = {2012}, abstract = {In vitro studies of the degradation kinetic of biopolymers are essential for the design and optimization of implantable biomedical devices. In the presented work, a field-effect capacitive sensor has been applied for the real-time and in situ monitoring of degradation processes of biopolymers for the first time. The polymer-covered field-effect sensor is, in principle, capable to detect any changes in bulk, surface and interface properties of the polymer induced by degradation processes. The feasibility of this approach has been experimentally proven by using the commercially available biomedical polymer poly(D,L-lactic acid) (PDLLA) as a model system. PDLLA films of different thicknesses were deposited on the Ta₂O₅-gate surface of the field-effect structure from a polymer solution by means of spin-coating method. The polymer-modified field-effect sensors have been characterized by means of capacitance-voltage and impedance-spectroscopy method. The degradation of the PDLLA was accelerated by changing the degradation medium from neutral (pH 7.2) to alkaline (pH 9) condition, resulting in drastic changes in the capacitance and impedance spectra of the polymer-modified field-effect sensor.}, language = {en} } @article{MukherjeeRamazaniYangetal.2011, author = {Mukherjee, Krishnendu and Ramazani, Ali and Yang, Li and Prahl, Ulrich and Bleck, Wolfgang and Reisgen, Uwe and Schleser, Markus and Abdurakhmanov, Aydemir}, title = {Characterization of gas metal arc welded hot rolled DP600 steel}, series = {Steel research international}, volume = {Vol. 82}, journal = {Steel research international}, number = {Iss. 12}, publisher = {Wiley}, address = {Weinheim}, issn = {1869-344X (E-Book); 1611-3683 (Print)}, pages = {1408 -- 1416}, year = {2011}, language = {en} } @article{FoersterMorvicBetkoetal.1999, author = {F{\"o}rster, Arnold and Morvic, M. and Betko, J. and Novak, J.}, title = {Characterization of low-temperature GaAs by conductivity, Hall effect and magnetoresistance measurements. Morvic, M., Betko, J.; Novak, J.; F{\"o}rster, A.; Kordos, P.}, series = {2nd Symposium on Non-Stoichiometric III-V Compounds : [4th - 6th October 1999, Erlangen] / [ed. by T. Marek ...] Friedrich-Alexander-Universit{\"a}t Erlangen-N{\"u}rnberg}, journal = {2nd Symposium on Non-Stoichiometric III-V Compounds : [4th - 6th October 1999, Erlangen] / [ed. by T. Marek ...] Friedrich-Alexander-Universit{\"a}t Erlangen-N{\"u}rnberg}, publisher = {Lehrstuhl f{\"u}r Mikrocharakterisierung}, address = {Erlangen}, isbn = {3-932392-19-1}, pages = {79 -- 85}, year = {1999}, language = {en} }