@article{ThustMulchandaniWangetal.2003, author = {Thust, Marion and Mulchandani, Ashok and Wang, Joseph and Arzdorf, Michael and Mulchandani, Priti and Chen, Wilfried and Sch{\"o}ning, Michael Josef}, title = {Feldeffekt-Enzymsensor zur Detektion von Pestiziden}, series = {Technisches Messen. 70 (2003), H. 12}, journal = {Technisches Messen. 70 (2003), H. 12}, isbn = {0171-8096}, pages = {561 -- 564}, year = {2003}, language = {de} } @article{WendlandtKochBritzetal.2023, author = {Wendlandt, Tim and Koch, Claudia and Britz, Beate and Liedek, Anke and Schmidt, Nora and Werner, Stefan and Gleba, Yuri and Vahidpour, Farnoosh and Welden, Melanie and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System}, series = {Viruses}, volume = {9}, journal = {Viruses}, number = {15}, publisher = {MDPI}, address = {Basel}, issn = {1999-4915}, doi = {doi.org/10.3390/v15091951}, pages = {Artikel 1951}, year = {2023}, abstract = {Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.}, language = {en} } @article{YoshinobuSchoeningFingeretal.2004, author = {Yoshinobu, T. and Sch{\"o}ning, Michael Josef and Finger, F. and Moritz, W. and Iwasaki, H.}, title = {Fabrication of thin-film LAPS with amorphous silicon}, series = {Sensors. 4 (2004), H. 10}, journal = {Sensors. 4 (2004), H. 10}, isbn = {1424-8220}, pages = {163 -- 169}, year = {2004}, language = {en} } @article{MuckWangJacobsetal.2004, author = {Muck, A. and Wang, J. and Jacobs, M. and Chen, G. and Chatrathi, M. P. and Jurka, V. and Vyborny, Z. and Spillmann, S. D. and Sridharan, G. and Sch{\"o}ning, Michael Josef}, title = {Fabrication of poly(methyl methacrylate) microfluidic chips by atmospheric molding}, series = {Analytical Chemistry. 76 (2004), H. 8}, journal = {Analytical Chemistry. 76 (2004), H. 8}, isbn = {0003-2700}, pages = {2290 -- 2297}, year = {2004}, language = {en} } @article{TakenagaSchneiderErbayetal.2015, author = {Takenaga, Shoko and Schneider, Benno and Erbay, E. and Biselli, Manfred and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef and Wagner, Torsten}, title = {Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201532053}, pages = {1347 -- 1352}, year = {2015}, abstract = {A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.}, language = {en} } @inproceedings{FoersterStockMontanarietal.2006, author = {F{\"o}rster, Arnold and Stock, J{\"u}rgen and Montanari, Simone and Lepsa, Mihail Ion and L{\"u}th, Hans}, title = {Fabrication and characterisation of GaAs Gunn Diode Chips for applications at 77 GHz in automotive industry}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-1462}, year = {2006}, abstract = {GaAs-based Gunn diodes with graded AlGaAs hot electron injector heterostructures have been developed under the special needs in automotive applications. The fabrication of the Gunn diode chips was based on total substrate removal and processing of integrated Au heat sinks. Especially, the thermal and RF behavior of the diodes have been analyzed by DC, impedance and S-parameter measurements. The electrical investigations have revealed the functionality of the hot electron injector. An optimized layer structure could fulfill the requirements in adaptive cruise control (ACC) systems at 77 GHz with typical output power between 50 and 90 mW.}, subject = {Biosensor}, language = {en} } @article{RibitschHeumannKarletal.2012, author = {Ribitsch, D. and Heumann, S. and Karl, W. and Gerlach, J. and Leber, R. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Siegert, Petra and Lange, J. and Maurer, Karl-Heinz and Berg, G. and Guebitz, G. M. and Schwab, H.}, title = {Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli}, series = {Journal of biotechnology}, volume = {157}, journal = {Journal of biotechnology}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2011.09.025}, pages = {140 -- 147}, year = {2012}, abstract = {A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.}, language = {en} } @article{SchrothSchoeningLuethetal.2001, author = {Schroth, P. and Sch{\"o}ning, Michael Josef and L{\"u}th, H. and Weißbecker, B. and Hummel, Hans E. and Sch{\"u}tz, S.}, title = {Extending the capabilities of an antenna/chip biosensor by employing various insect species}, series = {Sensors and Actuators B. 78 (2001), H. 1-3}, journal = {Sensors and Actuators B. 78 (2001), H. 1-3}, isbn = {0925-4005}, pages = {1 -- 5}, year = {2001}, language = {en} } @article{SchrothSchoeningLuethetal.2000, author = {Schroth, P. and Sch{\"o}ning, Michael Josef and L{\"u}th, H. and Weißbecker, B. and Hummel, Hans E. and Sch{\"u}tz, S.}, title = {Extending the capabilities of an antenna/chip biosensor by employing various insect species}, series = {Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]}, journal = {Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]}, publisher = {MIC, Mikroelektronik Centret}, address = {Lyngby, Denmark}, isbn = {87-89935-50-0}, pages = {27 -- 30}, year = {2000}, language = {en} } @inproceedings{OberlaenderJildehKirchneretal.2015, author = {Oberl{\"a}nder, Jan and Jildeh, Zaid B. and Kirchner, Patrick and Wendeler, Luisa and Bromm, Alexander and Iken, Heiko and Wagner, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Experimental and numerical evaluation of interdigitated electrode array for monitoring gaseous sterilization processes}, series = {12. Dresdner Sensor-Symposium 2015}, booktitle = {12. Dresdner Sensor-Symposium 2015}, doi = {10.5162/12dss2015/P3.11}, pages = {163 -- 168}, year = {2015}, language = {en} } @article{JildehOberlaenderKirchneretal.2018, author = {Jildeh, Zaid B. and Oberl{\"a}nder, Jan and Kirchner, Patrick and Keusgen, Michael and Wagner, Patrick H. and Sch{\"o}ning, Michael Josef}, title = {Experimental and Numerical Analyzes of a Sensor Based on Interdigitated Electrodes for Studying Microbiological Alterations}, series = {physica status solidi (a): applications and materials science}, volume = {215}, journal = {physica status solidi (a): applications and materials science}, number = {15}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201700920}, pages = {Artikel 1700920}, year = {2018}, abstract = {In this work, a cell-based biosensor to evaluate the sterilization efficacy of hydrogen peroxide vapor sterilization processes is characterized. The transducer of the biosensor is based on interdigitated gold electrodes fabricated on an inert glass substrate. Impedance spectroscopy is applied to evaluate the sensor behavior and the alteration of test microorganisms due to the sterilization process. These alterations are related to changes in relative permittivity and electrical conductivity of the bacterial spores. Sensor measurements are conducted with and without bacterial spores (Bacillus atrophaeus), as well as after an industrial sterilization protocol. Equivalent two-dimensional numerical models based on finite element method of the periodic finger structures of the interdigitated gold electrodes are designed and validated using COMSOL® Multiphysics software by the application of known dielectric properties. The validated models are used to compute the electrical properties at different sensor states (blank, loaded with spores, and after sterilization). As a final result, we will derive and tabulate the frequency-dependent electrical parameters of the spore layer using a novel model that combines experimental data with numerical optimization techniques.}, language = {en} } @article{SiegertMcLeishBaumannetal.2005, author = {Siegert, Petra and McLeish, Michael J. and Baumann, Martin and Iding, Hans and Kneen, Malea M. and Kenyon, George L. and Pohl, Martina}, title = {Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida}, series = {Protein engineering, design, and selection : peds}, volume = {Vol. 18}, journal = {Protein engineering, design, and selection : peds}, number = {Iss. 7}, issn = {1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print)}, pages = {345 -- 357}, year = {2005}, language = {en} } @article{SchoeningSimonisKringsetal.2002, author = {Sch{\"o}ning, Michael Josef and Simonis, A. and Krings, T. and L{\"u}th, H. and Wang, J.}, title = {Evaluation of a chip-based thin-film / thick-film sensor hybrid for (bio-)chemical analysis}, series = {Electroanalysis}, volume = {14}, journal = {Electroanalysis}, number = {13}, issn = {1040-0397}, pages = {955 -- 958}, year = {2002}, language = {en} } @article{BohrnStuetzFleischeretal.2011, author = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {1345 -- 1350}, year = {2011}, language = {en} } @inproceedings{BuniatyanHuckPoghossianetal.2014, author = {Buniatyan, V. V. and Huck, Christina and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Rustamyan, L. G. and Hovnikyan, H. H.}, title = {Equivalent circuit and optimization of impedance characteristics of an electrolyte conductivity sensor}, series = {Proceedings of State Engineering University Armenia : Series Information technologies, electronics, radio engineering}, volume = {Iss. 17}, booktitle = {Proceedings of State Engineering University Armenia : Series Information technologies, electronics, radio engineering}, number = {No. 1}, pages = {69 -- 76}, year = {2014}, language = {en} } @article{BandodkarMolinnusMirzaetal.2014, author = {Bandodkar, Amay J. and Molinnus, Denise and Mirza, Omar and Guinovart, Tomas and Windmiller, Joshua R. and Valdes-Ramirez, Gabriela and Andrade, Francisco J. and Sch{\"o}ning, Michael Josef and Wang, Joseph}, title = {Epidermal tattoo potentiometric sodium sensors with wireless signal transduction for continuous non-invasive sweat monitoring}, series = {Biosensors and bioelectronics}, volume = {54}, journal = {Biosensors and bioelectronics}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4235 (E-Journal); 0956-5663 (Print)}, doi = {10.1016/j.bios.2013.11.039}, pages = {603 -- 609}, year = {2014}, abstract = {This article describes the fabrication, characterization and application of an epidermal temporary-transfer tattoo-based potentiometric sensor, coupled with a miniaturized wearable wireless transceiver, for real-time monitoring of sodium in the human perspiration. Sodium excreted during perspiration is an excellent marker for electrolyte imbalance and provides valuable information regarding an individual's physical and mental wellbeing. The realization of the new skin-worn non-invasive tattoo-like sensing device has been realized by amalgamating several state-of-the-art thick film, laser printing, solid-state potentiometry, fluidics and wireless technologies. The resulting tattoo-based potentiometric sodium sensor displays a rapid near-Nernstian response with negligible carryover effects, and good resiliency against various mechanical deformations experienced by the human epidermis. On-body testing of the tattoo sensor coupled to a wireless transceiver during exercise activity demonstrated its ability to continuously monitor sweat sodium dynamics. The real-time sweat sodium concentration was transmitted wirelessly via a body-worn transceiver from the sodium tattoo sensor to a notebook while the subjects perspired on a stationary cycle. The favorable analytical performance along with the wearable nature of the wireless transceiver makes the new epidermal potentiometric sensing system attractive for continuous monitoring the sodium dynamics in human perspiration during diverse activities relevant to the healthcare, fitness, military, healthcare and skin-care domains.}, language = {en} } @article{TurekKeusgenPoghossianetal.2008, author = {Turek, M. and Keusgen, M. and Poghossian, Arshak and Mulchandani, A. and Wang, J. and Sch{\"o}ning, Michael Josef}, title = {Enzyme-modified electrolyte-insulator-semiconductor sensors}, series = {Journal of Contemporary Physics. 43 (2008), H. 2}, journal = {Journal of Contemporary Physics. 43 (2008), H. 2}, isbn = {1934-9378}, pages = {82 -- 85}, year = {2008}, language = {en} } @article{KatzPoghossianSchoening2017, author = {Katz, Evgeny and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Enzyme-based logic gates and circuits - analytical applications and interfacing with electronics}, series = {Analytical and Bioanalytical Chemistry}, volume = {409}, journal = {Analytical and Bioanalytical Chemistry}, publisher = {Springer}, address = {Berlin}, issn = {1618-2650}, doi = {10.1007/s00216-016-0079-7}, pages = {81 -- 94}, year = {2017}, abstract = {The paper is an overview of enzyme-based logic gates and their short circuits, with specific examples of Boolean AND and OR gates, and concatenated logic gates composed of multi-step enzyme-biocatalyzed reactions. Noise formation in the biocatalytic reactions and its decrease by adding a "filter" system, converting convex to sigmoid response function, are discussed. Despite the fact that the enzyme-based logic gates are primarily considered as components of future biomolecular computing systems, their biosensing applications are promising for immediate practical use. Analytical use of the enzyme logic systems in biomedical and forensic applications is discussed and exemplified with the logic analysis of biomarkers of various injuries, e.g., liver injury, and with analysis of biomarkers characteristic of different ethnicity found in blood samples on a crime scene. Interfacing of enzyme logic systems with modified electrodes and semiconductor devices is discussed, giving particular attention to the interfaces functionalized with signal-responsive materials. Future perspectives in the design of the biomolecular logic systems and their applications are discussed in the conclusion.}, language = {en} } @incollection{SchoeningPoghossian2018, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {Enzyme und Biosensorik}, series = {Einf{\"u}hrung in die Enzymtechnologie}, booktitle = {Einf{\"u}hrung in die Enzymtechnologie}, publisher = {Springer Spektrum}, address = {Berlin}, isbn = {978-3-662-57619-9}, doi = {10.1007/978-3-662-57619-9_18}, pages = {323 -- 347}, year = {2018}, abstract = {Enzymbasierte Biosensoren finden seit mehr als f{\"u}nf Jahrzehnten einen prosperierenden Wachstumsmarkt und werden zunehmend auch in biotechnologischen Prozessen eingesetzt. In diesem Kapitel werden, ausgehend vom Sensorbegriff und typischen Kenngr{\"o}ßen f{\"u}r Biosensoren (Abschn. 18.1), elektrochemische Enzym-Biosensoren vorgestellt und deren typischen Einsatzgebiete diskutiert (Abschn. 18.2). Ein Blick {\"u}ber den „Tellerrand" hinaus zeigt alternative Transduktorprinzipien (Abschn. 18.3) und f{\"u}hrt abschließend in aktuelle Forschungstrends ein (Abschn. 18.4).}, language = {de} } @article{PoghossianKatzSchoening2015, author = {Poghossian, Arshak and Katz, Evgeny and Sch{\"o}ning, Michael Josef}, title = {Enzyme logic AND-Reset and OR-Reset gates based on a field-effect electronic transducer modified with multi-enzyme membrane}, series = {Chemical Communications}, volume = {51}, journal = {Chemical Communications}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, doi = {10.1039/C5CC01362C}, pages = {6564 -- 6567}, year = {2015}, abstract = {Capacitive field-effect sensors modified with a multi-enzyme membrane have been applied for an electronic transduction of biochemical signals processed by enzyme-based AND-Reset and OR-Reset logic gates. The local pH change at the sensor surface induced by the enzymatic reaction was used for the activation of the Reset function for the first time.}, language = {en} }