@article{KapplerTanudyayaSchmittTippkoetteretal.2007, author = {Kappler-Tanudyaya, Nathalie and Schmitt, Heike and Tippk{\"o}tter, Nils and Meyer, Lina and Lenzen, Sigurd and Ulber, Roland}, title = {Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose}, series = {Biotechnology Journal}, volume = {2}, journal = {Biotechnology Journal}, number = {6}, issn = {1860-7314}, doi = {10.1002/biot.200700004}, pages = {692 -- 699}, year = {2007}, abstract = {Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently.}, language = {en} } @article{PaulssenKueckmannAbram2007, author = {Paulßen, Elisabeth and K{\"u}ckmann, Theresa and Abram, Ulrich}, title = {Silver(I) Complexes of 1,3-Dialkyl-4,5-dimethylimidazol-2-ylidenes and their Use as Precursors for the Synthesis of Rhenium(V) NHC Complexes}, series = {Zeitschrift f{\"u}r anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry}, volume = {633}, journal = {Zeitschrift f{\"u}r anorganische und allgemeine Chemie : ZAAC = Journal of inorganic and general chemistry}, number = {5-6}, issn = {1521-3749}, doi = {10.1002/zaac.200700021}, pages = {830 -- 834}, year = {2007}, language = {en} } @inproceedings{TippkoetterStueckmannWinkelmannetal.2007, author = {Tippk{\"o}tter, Nils and St{\"u}ckmann, H. and Winkelmann, G. and Noack, U. and Beutel, S. and Scheper, T. and Ulber, R.}, title = {Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR}, series = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme}, booktitle = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme}, publisher = {Dechema}, address = {Frankfurt am Main}, pages = {126}, year = {2007}, language = {en} } @article{WallerBraunHojdisetal.2007, author = {Waller, Mark P. and Braun, Heiko and Hojdis, Nils and B{\"u}hl, Michael}, title = {Geometries of Second-Row Transition-Metal Complexes from Density-Functional Theory}, series = {Journal of Chemical Theory and Computation}, volume = {3}, journal = {Journal of Chemical Theory and Computation}, number = {6}, issn = {1549-9626}, doi = {10.1021/ct700178y}, pages = {2234 -- 2242}, year = {2007}, language = {en} } @article{JerominZoor2008, author = {Jeromin, G{\"u}nter Erich and Zoor, Annegreth}, title = {A new irreversible enzyme-aided esterification method in organic solvents}, series = {Biotechnology letters. 30 (2008), H. 5}, journal = {Biotechnology letters. 30 (2008), H. 5}, isbn = {1573-6776}, pages = {925 -- 928}, year = {2008}, language = {en} } @article{SelmerPinkenburg2008, author = {Selmer, Thorsten and Pinkenburg, Olaf}, title = {Method of cloning at least one nucleic acid molecule of interest using type IIS restriction endonucleases, and corresponding cloning vectors, kits and system using type IIS restriction endonucleases / Selmer, Thorsten ; Pinkenburg, Olaf}, year = {2008}, language = {en} } @article{HeroldBrandtSeibleretal.2008, author = {Herold, Marco J. and Brandt, Jens van den and Seibler, Jost and Reichardt, Holger M.}, title = {Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats}, series = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {105}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, number = {47}, issn = {1091-6490}, doi = {10.1073/pnas.0806213105}, pages = {18507 -- 18512}, year = {2008}, language = {en} } @article{KochWunderlichSeibleretal.2008, author = {Koch, Linda and Wunderlich, F. Thomas and Seibler, Jost and K{\"o}nner, A. Christine and Hampel, Brigitte and Irlenbusch, Sigrid and Brabant, Georg and Kahn, C. Ronald and Schwenk, Frieder and Br{\"u}ning, Jens C.}, title = {Central insulin action regulates peripheral glucose and fat metabolism in mice}, series = {The Journal of Clinical Investigation (JCI)}, volume = {118}, journal = {The Journal of Clinical Investigation (JCI)}, number = {6}, issn = {1558-8238}, doi = {10.1172/JCI31073}, pages = {2132 -- 2147}, year = {2008}, language = {en} } @article{ChristophBahrenbergVryetal.2008, author = {Christoph, Thomas and Bahrenberg, Gregor and Vry, Jean de and Englberger, Werner and Erdmann, Volker A. and Frech, Moritz and K{\"o}gel, Babette and R{\"o}hl, Thomas and Schiene, Klaus and Schr{\"o}der, Wolfgang and Seibler, Jost and Kurreck, Jens}, title = {Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice}, series = {Molecular and Cellular Neuroscience}, volume = {37}, journal = {Molecular and Cellular Neuroscience}, number = {3}, issn = {1044-7431}, doi = {10.1016/j.mcn.2007.12.006}, pages = {579 -- 589}, year = {2008}, language = {en} } @article{TippkoetterDeterdingUlber2008, author = {Tippk{\"o}tter, Nils and Deterding, A. and Ulber, Roland}, title = {Determination of acetic acid in fermentation broth by gas-diffusion technique}, series = {Engineering in Life Sciences}, volume = {8}, journal = {Engineering in Life Sciences}, number = {1, Special Issue: Technical Systems for the Use in Life Sciences}, doi = {10.1002/elsc.200820227}, pages = {62 -- 67}, year = {2008}, abstract = {Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5-5.0 g/L acetic acid with a relative standard deviation of <5 \% was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.}, language = {en} } @article{TippkoetterRoikaewUlber2008, author = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, R.}, title = {Nitrate removal from whey concentrate with biotechnological regeneration of the waste water}, series = {European dairy magazine : EDM}, journal = {European dairy magazine : EDM}, number = {1}, isbn = {0936-6318}, pages = {30 -- 32}, year = {2008}, language = {en} } @inproceedings{TippkoetterRoikaewUlber2008, author = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, R.}, title = {An automated pilot plant for the bioengineering processing of concentrated whey}, series = {European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme}, booktitle = {European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme}, publisher = {Dechema}, address = {Frankfurt am Main}, pages = {98}, year = {2008}, language = {en} } @article{ScheerRossRodeetal.2008, author = {Scheer, Nico and Ross, Jillian and Rode, Anja and Zevnik, Branko and Niehaves, Sandra and Faust, Nicole and Wolf, C. Roland}, title = {A novel panel of mouse models to evaluate the role of human pregnane X receptor and constitutive androstane receptor in drug response}, series = {Journal of Clinical Investigation}, volume = {118}, journal = {Journal of Clinical Investigation}, number = {9}, issn = {1558-8238}, doi = {https://doi.org/10.1172/JCI35483}, pages = {3228 -- 3239}, year = {2008}, language = {en} } @article{MangHodeniusSchmitzRodeetal.2009, author = {Mang, Thomas and Hodenius, M. A. J. and Schmitz-Rode, T. and Baumann, M.}, title = {Absorption of 10-hydroxycamptothecin into the coat of magnetoliposomes / Hodenius, M. A. J. ; Schmitz-Rode, T. ; Baumann, M. ; Ivoanova, G. ; Wong, J. E. ; Mang, T. ; Haulena, F. ; Soenen, S. J. H. ; De Cuyper, M.}, series = {Colloids and Surfaces A: Physicochemical and Engineering Aspects. 343 (2009), H. 1-3}, journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects. 343 (2009), H. 1-3}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0927-7757}, pages = {20 -- 23}, year = {2009}, language = {en} } @article{SchererJacobiCastilloetal.2009, author = {Scherer, Ulrich W. and Jacobi, M. and Castillo, J. and Foerstel, D. H.}, title = {Ultra-low-level measurements of 3H and 14C in wines and champagne / Scherer, U. W. ; Jacobi, M. ; Castillo, J. ; Foerstel, D. H.}, series = {Radiation effects and defects in solids. 164 (2009), H. 5-6}, journal = {Radiation effects and defects in solids. 164 (2009), H. 5-6}, isbn = {1042-0150}, pages = {382 -- 385}, year = {2009}, language = {en} } @article{SchererSantanaMaieretal.2009, author = {Scherer, Ulrich W. and Santana, H. H. S. and Maier, G. and Rodenas, J.}, title = {Analysis of mechanical strength in ceramic pellets of nuclear fuel / Santana, H. H. S. ; Maier, G. ; Scherer, U. W. ; Rodenas, J.}, series = {Radiation effects and defects in solids. 164 (2009), H. 5-6}, journal = {Radiation effects and defects in solids. 164 (2009), H. 5-6}, publisher = {Taylor \& Francis}, address = {London}, isbn = {1042-0150}, pages = {313 -- 318}, year = {2009}, language = {en} } @article{Elbers2009, author = {Elbers, Gereon}, title = {Identification of marker peptides in hair for discrimination with regard to species and breed / Koerner, Andrea ; Ring, Maike ; Fabry, Marlies ; Elbers, Gereon ; Moeller, Martin}, series = {Proceedings of the Aachen-Dresden International Textile Conference, 3rd, Aachen, Germany, Nov. 26-27, 2009}, journal = {Proceedings of the Aachen-Dresden International Textile Conference, 3rd, Aachen, Germany, Nov. 26-27, 2009}, publisher = {DWI}, address = {Aachen}, pages = {1 CD-ROM}, year = {2009}, language = {en} } @article{BaeckerBegingBisellietal.2009, author = {B{\"a}cker, Matthias and Beging, Stefan and Biselli, Manfred and Poghossian, Arshak and Wang, J. and Zang, Werner and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Concept for a solid-state multi-parameter sensor system for cell-culture monitoring}, series = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, journal = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0013-4686}, pages = {6107 -- 6112}, year = {2009}, language = {en} } @article{Jeromin2009, author = {Jeromin, G{\"u}nter Erich}, title = {Superabsorbed alcohol dehydrogenase—a new catalyst for asymmetric reductions}, series = {Biotechnology Letters. 31 (2009), H. 11}, journal = {Biotechnology Letters. 31 (2009), H. 11}, isbn = {0141-5492}, pages = {1717 -- 1721}, year = {2009}, language = {en} } @article{OehlschlaegerQuettingAlvarezetal.2009, author = {{\"O}hlschl{\"a}ger, Peter and Quetting, Michael and Alvarez, Gerardo and D{\"u}rst, Matthias and Gissmann, Lutz and Kaufmann, Andreas M.}, title = {Enhancement of immunogenicity of a therapeutic cervical cancer DNA-based vaccine by co-application of sequence-optimized genetic adjuvants}, series = {International Journal of Cancer}, volume = {125}, journal = {International Journal of Cancer}, number = {1}, publisher = {Wiley}, address = {Weinheim}, isbn = {1097-0215}, pages = {189 -- 198}, year = {2009}, language = {en} }