@article{WagnerMiyamotoSchoeningetal.2010, author = {Wagner, Torsten and Miyamoto, Ko-ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Novel combination of digital light processing (DLP) and light-addressable potentiometric sensors (LAPS) for flexible chemical imaging}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {520 -- 523}, year = {2010}, language = {en} } @article{KirchnerOberlaenderFriedrichetal.2010, author = {Kirchner, Patrick and Oberl{\"a}nder, Jan and Friedrich, Peter and Rysstad, G. and Berger, J. and Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Realization of a calorimetric gas sensor on polyimide foil for applications in aseptic food industry}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {264 -- 267}, year = {2010}, language = {en} } @article{MiyamotoKanekoMatsuoetal.2010, author = {Miyamoto, Ko-ichiro and Kaneko, Kazumi and Matsuo, Akira and Wagner, Torsten and Kanoh, Shin`ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Miniaturized chemical imaging sensor system using an OLED display panel}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {516 -- 519}, year = {2010}, language = {en} } @article{BohrnStuetzFleischeretal.2010, author = {Bohrn, U. and St{\"u}tz, E. and Fleischer, M. and Sch{\"o}ning, Michael Josef}, title = {Real-time detection of CO by eukaryotic cells}, series = {Procedia Engineering. 5 (2010)}, journal = {Procedia Engineering. 5 (2010)}, isbn = {1877-7058}, pages = {17 -- 20}, year = {2010}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @inproceedings{BegingPoghossianMlyneketal.2010, author = {Beging, Stefan and Poghossian, Arshak and Mlynek, D. and Hataihimakul, S. and Pedraza, A. and Dhawan, S. and Laube, N. and Kleinen, L. and Baldsiefen, G. and Busch, H. and Sch{\"o}ning, Michael Josef}, title = {Ion-selective sensors for the determination of the risk of urinary stone formation}, series = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, booktitle = {Micro- and Nanosystems in biochemical diagnosis : Principles and applications}, address = {Warsaw}, pages = {74 -- 80}, year = {2010}, language = {en} } @article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @inproceedings{SchusserVaessenSchoening2011, author = {Schusser, Sebastian and Vaeßen, Christiane and Sch{\"o}ning, Michael Josef}, title = {4. Graduiertentagung der FH Aachen 24. November 2011}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-3530}, year = {2011}, abstract = {Tagungsband der 4. Graduiertentagung der FH Aachen am 24. November 2011 Proceedings from the 4th Graduate Symposium, FH Aachen, Germany, November 24th, 2011 Aachen, November 2011. 62 Seiten}, subject = {Graduiertentagung}, language = {de} } @article{WernerSchusserSpalthahnetal.2011, author = {Werner, Frederik and Schusser, Sebastian and Spalthahn, Heiko and Wagner, Torsten and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {Field-programmable gate array based controller for multi spot light-addressable potentiometric sensors with integrated signal correction mode}, series = {Electrochimica Acta}, volume = {56}, journal = {Electrochimica Acta}, number = {26}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2011.03.012}, pages = {9656 -- 9660}, year = {2011}, abstract = {A light-addressable potentiometric sensor (LAPS) can measure the concentration of one or several analytes at the sensor surface simultaneously in a spatially resolved manner. A modulated light pointer stimulates the semiconductor structure at the area of interest and a responding photocurrent can be read out. By simultaneous stimulation of several areas with light pointers of different modulation frequencies, the read out can be performed at the same time. With the new proposed controller electronic based on a field-programmable gate array (FPGA), it is possible to control the modulation frequencies, phase shifts, and light brightness of multiple light pointers independently and simultaneously. Thus, it is possible to investigate the frequency response of the sensor, and to examine the analyte concentration by the determination of the surface potential with the help of current/voltage curves and phase/voltage curves. Additionally, the ability to individually change the light intensities of each light pointer is used to perform signal correction.}, language = {en} } @article{AbouzarPoghossianPedrazaetal.2011, author = {Abouzar, Maryam H. and Poghossian, Arshak and Pedraza, A. M. and Gandhi, D. and Ingebrandt, S. and Moritz, W. and Sch{\"o}ning, Michael Josef}, title = {An array of field-effect nanoplate SOI capacitors for (bio-)chemical sensing}, series = {Biosensors and Bioelectronics. 26 (2011), H. 6}, journal = {Biosensors and Bioelectronics. 26 (2011), H. 6}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0956-5663}, pages = {3023 -- 3028}, year = {2011}, language = {en} } @article{KirchnerLiSpelthahnetal.2011, author = {Kirchner, Patrick and Li, Bin and Spelthahn, Heiko and Henkel, Hartmut and Schneider, Andreas and Friedrich, Peter and Kolstad, Jens and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Thin-film calorimetric H2O2 gas sensor for the validation of germicidal effectivity in aseptic filling processes}, series = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, journal = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1873-3077}, pages = {257 -- 263}, year = {2011}, language = {en} } @article{MiyamotoWagnerYoshinobuetal.2011, author = {Miyamoto, Ko-ichiro and Wagner, Torsten and Yoshinobu, Tatsuo and Kanoh, Shin`ichiro and Sch{\"o}ning, Michael Josef}, title = {Phase-mode LAPS and its application to chemical imaging}, series = {Sensors and Actuators B: Chemical. 154 (2011), H. 1}, journal = {Sensors and Actuators B: Chemical. 154 (2011), H. 1}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1873-3077}, pages = {28 -- 32}, year = {2011}, language = {en} } @article{MiyamotoYoshidaSakaietal.2011, author = {Miyamoto, Ko-ichiro and Yoshida, Midori and Sakai, Taito and Matsuzaka, Atsushi and Wagner, Torsten and Kanoh, Sanoh and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {Differential setup of light-addressable potentiometric sensor with an enzyme reactor in a flow channel}, series = {Japanese Journal of Applied Physics. 50 (2011)}, journal = {Japanese Journal of Applied Physics. 50 (2011)}, publisher = {Japan Society of Applied Physics}, address = {Bristol}, isbn = {0021-4922}, pages = {04DL08-1 -- 04DL08-5}, year = {2011}, language = {en} } @article{GasparyanVitusevichOffenhaeusseretal.2011, author = {Gasparyan, F.V. and Vitusevich, S.A. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef}, title = {Modified charge fluctuation noise model for electrolyte-insulator-semiconductor devices}, series = {Modern Physics Letters B (MPLB). 25 (2011), H. 11}, journal = {Modern Physics Letters B (MPLB). 25 (2011), H. 11}, publisher = {World Scientific Publ.}, address = {Singapur}, isbn = {0217-9849}, pages = {831 -- 840}, year = {2011}, language = {en} } @article{GrinsvenBonGrietenetal.2011, author = {Grinsven, B. van and Bon, N. vanden and Grieten, L. and Murib, M. and Janssen, S. D. and Haenen, K. and Schneider, E. and Ingebrandt, E. and Sch{\"o}ning, Michael Josef and Vermeeren, V. and Ameloot, M. and Michiels, L. and Thoelen, R. and Ceuninck, W. de and Wagner, P.}, title = {Rapid assessment of the stability of DNA duplexes by impedimetric real-time monitoring of chemically induced denaturation}, series = {Lab on a Chip}, volume = {11}, journal = {Lab on a Chip}, number = {9}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, isbn = {1473-0197}, pages = {1656 -- 1663}, year = {2011}, language = {en} } @article{GunGutkinLevetal.2011, author = {Gun, Jenny and Gutkin, Vitaly and Lev, Ovadia and Boyen, Hans-Gerd and Saitner, Marc and Wagner, Patrick and Olieslaeger, Marc D´ and Abouzar, Maryam H. and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Tracing gold nanoparticle charge by electrolyte-insulator-semiconductor devices}, series = {Journal of Physical Chemistry C. 115 (2011), H. 11}, journal = {Journal of Physical Chemistry C. 115 (2011), H. 11}, publisher = {American Cemical Society}, address = {Washington, DC}, isbn = {1932-7455}, pages = {4439 -- 4445}, year = {2011}, language = {en} } @article{MiyamotoWagnerYoshinobuetal.2011, author = {Miyamoto, Ko-ichiro and Wagner, Torsten and Yoshinobu, Tatsuo and Kanoh, Shin`ichiro and Sch{\"o}ning, Michael Josef}, title = {Phase-mode operation of FDM-LAPS}, series = {Sensor letters}, volume = {9}, journal = {Sensor letters}, number = {2}, publisher = {American Scientific Publishers}, address = {Stevenson Ranch, Calif.}, isbn = {1546-1971}, pages = {691 -- 694}, year = {2011}, language = {en} } @article{BohrnStuetzFleischeretal.2011, author = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {1345 -- 1350}, year = {2011}, language = {en} } @article{BaeckerPouyeshmanSchnitzleretal.2011, author = {B{\"a}cker, Matthias and Pouyeshman, S. and Schnitzler, Thomas and Poghossian, Arshak and Wagner, Patrick and Biselli, Manfred and Sch{\"o}ning, Michael Josef}, title = {A silicon-based multi-sensor chip for monitoring of fermentation processes}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {1364 -- 1369}, year = {2011}, language = {en} } @article{WernerKrumbeSchumacheretal.2011, author = {Werner, Frederik and Krumbe, Christoph and Schumacher, Katharina and Groebel, Simone and Spelthahn, Heiko and Stellberg, Michael and Wagner, Torsten and Yoshinobu, Tatsuo and Selmer, Thorsten and Keusgen, Michael and Baumann, Marcus and Sch{\"o}ning, Michael Josef}, title = {Determination of the extracellular acidification of Escherichia coli by a light-addressable potentiometric sensor}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {1340 -- 1344}, year = {2011}, language = {en} }