@article{SiekerNeunerDimitrovaetal.2011, author = {Sieker, Tim and Neuner, Andreas and Dimitrova, Darina and Tippk{\"o}tter, Nils and Muffler, Kai and Bart, Hans-J{\"o}rg and Heinzle, Elmar and Ulber, Roland}, title = {Ethanol production from grass silage by simultaneous pretreatment, saccharification and fermentation: First steps in the process development}, series = {Engineering in Life Sciences}, volume = {11}, journal = {Engineering in Life Sciences}, number = {4}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/elsc.201000160}, pages = {436 -- 442}, year = {2011}, abstract = {Grass silage provides a great potential as renewable feedstock. Two fractions of the grass silage, a press juice and the fiber fraction, were evaluated for their possible use for bioethanol production. Direct production of ethanol from press juice is not possible due to high concentrations of organic acids. For the fiber fraction, alkaline peroxide or enzymatic pretreatment was used, which removes the phenolic acids in the cell wall. In this study, we demonstrate the possibility to integrate the enzymatic pretreatment with a simultaneous saccharification and fermentation to achieve ethanol production from grass silage in a one-process step. Achieved yields were about 53 g ethanol per kg silage with the alkaline peroxide pretreatment and 91 g/kg with the enzymatic pretreatment at concentrations of 8.5 and 14.6 g/L, respectively. Furthermore, it was shown that additional supplementation of the fermentation medium with vitamins, trace elements and nutrient salts is not necessary when the press juice is directly used in the fermentation step.}, language = {en} } @article{PothMonzonTippkoetteretal.2011, author = {Poth, Sebastian and Monzon, Magaly and Tippk{\"o}tter, Nils and Ulber, Roland}, title = {Lignocellulosic biorefinery: Process integration of hydrolysis and fermentation (SSF process)}, series = {Holzforschung}, volume = {65}, journal = {Holzforschung}, number = {5}, publisher = {De Gruyter}, address = {Berlin}, pages = {633 -- 637}, year = {2011}, abstract = {The aim of the present work is the process integration and the optimization of the enzymatic hydrolysis of wood and the following fermentation of the products to ethanol. The substrate is a fiber fraction obtained by organosolv pre-treatment of beech wood. For the ethanol production, a co-fermentation by two different yeasts (Saccharomyces cerevisiae and Pachysolen tannophilus) was carried out to convert glucose as well as xylose. Two approaches has been followed: 1. A two step process, in which the hydrolysis of the fiber fraction and the fermentation to product are separated from each other. 2. A process, in which the hydrolysis and the fermentation are carried out in one single process step as simultaneous saccharification and fermentation (SSF). Following the first approach, a yield of about 0.15 g ethanol per gram substrate can be reached. Based on the SSF, one process step can be saved, and additionally, the gained yield can be raised up to 0.3 g ethanol per gram substrate.}, language = {en} } @incollection{HahnKellyMuffleretal.2011, author = {Hahn, Thomas and Kelly, Svenja and Muffler, Kai and Tippk{\"o}tter, Nils and Ulber, Roland}, title = {Extraction of lignocellulose and algae for the production of bulk and fine chemicals}, series = {Industrial scale natural products extraction}, booktitle = {Industrial scale natural products extraction}, editor = {Hans-J{\"o}rg, Bart and Pilz, Stephan}, publisher = {Wiley-VCH}, address = {Weinheim}, isbn = {978-3-527-32504-7 (Print)}, doi = {10.1002/9783527635122}, pages = {221 -- 245}, year = {2011}, language = {en} } @incollection{MufflerPothSiekeretal.2011, author = {Muffler, Kai and Poth, Sabastian and Sieker, Tim and Tippk{\"o}tter, Nils and Ulber, Roland and Sell, Dieter}, title = {Bio-feedstocks}, series = {Comprehensive biotechnology : principles and practices in industry, agcriculture, medicine and the environment. Volume 2: Engineering fundamentals of biotechnology}, booktitle = {Comprehensive biotechnology : principles and practices in industry, agcriculture, medicine and the environment. Volume 2: Engineering fundamentals of biotechnology}, editor = {Moo-Young, Murray}, edition = {2. edition}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {978-0-444-53352-4}, doi = {10.1016/B978-0-08-088504-9.00088-X}, pages = {93 -- 101}, year = {2011}, language = {en} } @article{TippkoetterWollnyKampeisetal.2011, author = {Tippk{\"o}tter, Nils and Wollny, S. and Kampeis, P. and Oster, J. and Schneider, H. and Ulber, R.}, title = {Magnetseparation von Proteinen : Separation von Zielmolek{\"u}len durch hochselektive Aptamere}, series = {GIT Labor-Fachzeitschrift}, volume = {55}, journal = {GIT Labor-Fachzeitschrift}, number = {10}, publisher = {Wiley}, address = {Weinheim}, pages = {666}, year = {2011}, abstract = {Durch die Kombination von Oligonukleotid-Liganden (Aptameren) hoher Bindungsaffinit{\"a}ten mit hochselektiv abtrennbaren magnetisierbaren Mikropartikeln wird eine einstufige Separation von Zielmolek{\"u}len aus mikrobiologischen Produktionsans{\"a}tzen m{\"o}glich. Die Aptamere werden hierf{\"u}r reversibel auf den Partikeloberfl{\"a}chen gebunden und f{\"u}r die spezifische Isolierung von Bioprodukten eingesetzt. Die Abtrennung der beladenen Partikel erfolgt durch einen neuen Rotor-Stator-Separator mit Hochgradient-Magnetfeld.}, language = {de} } @incollection{UlberMufflerTippkoetteretal.2011, author = {Ulber, Roland and Muffler, Kai and Tippk{\"o}tter, Nils and Hirth, Thomas and Sell, Dieter}, title = {Introduction to Renewable Resources in the Chemical Industry}, series = {Renewable raw materials : new feedstocks for the chemical industry}, booktitle = {Renewable raw materials : new feedstocks for the chemical industry}, editor = {Ulber, Roland and Sell, Dieter and Hirth, Thomas}, edition = {1. Auflage}, publisher = {Wiley-VCH-Verlag}, address = {Weinheim}, isbn = {978-3-527-32548-1}, pages = {1 -- 6}, year = {2011}, language = {de} } @article{ImmelGrothHuhnetal.2011, author = {Immel, Timo A. and Groth, Ulrich and Huhn, Thomas and {\"O}hlschl{\"a}ger, Peter}, title = {Titanium salan complexes displays strong antitumor properties in vitro and in vivo in mice}, series = {PLoS ONE}, volume = {6}, journal = {PLoS ONE}, number = {3}, publisher = {Plos}, address = {San Francisco, California, US}, doi = {10.1371/journal.pone.0017869}, pages = {e17869}, year = {2011}, abstract = {The anticancer activity of titanium complexes has been known since the groundbreaking studies of K{\"o}pf and K{\"o}pf-Maier on titanocen dichloride. Unfortunately, possibly due to their fast hydrolysis, derivatives of titanocen dichloride failed in clinical studies. Recently, the new family of titanium salan complexes containing tetradentate ONNO ligands with anti-cancer properties has been discovered. These salan complexes are much more stabile in aqueous media. In this study we describe the biological activity of two titanium salan complexes in a mouse model of cervical cancer. High efficiency of this promising complex family was demonstrated for the first time in vivo. From these data we conclude that titanium salan complexes display very strong antitumor properties exhibiting only minor side effects. Our results may influence the chemotherapy with metallo therapeutics in the future.}, language = {en} } @article{BalakrishnanAndreiSelmerSelmeretal.2010, author = {Balakrishnan, Karthikeyan and Andrei-Selmer, Luminita-Cornelia and Selmer, Thorsten and Bacher, Michael and Dodel, Richard}, title = {Comparison of Intravenous Immunoglobulins for Naturally Occurring Autoantibodies against Amyloid-β}, series = {Journal of Alzheimer's Disease}, volume = {20}, journal = {Journal of Alzheimer's Disease}, number = {1}, isbn = {1387-2877}, pages = {135 -- 143}, year = {2010}, language = {en} } @misc{Jeromin2010, author = {Jeromin, G{\"u}nter Erich}, title = {Immobilisierung von Alkoholdehydrogenasen und deren Coenzyme sowie Verwendung des Immobilisats : Offenlegungsschrift : DE 102008038326 A1 Offenlegungstag: 25.02.2010}, publisher = {Deutsches Patent- und Markenamt}, address = {M{\"u}nchen}, pages = {6 S.}, year = {2010}, language = {de} } @article{KowollikSchnitzlerBisellietal.2010, author = {Kowollik, S. and Schnitzler, Thomas and Biselli, Manfred and Krueger, R. and Zang, Werner and Peuscher, A. and Schillberg, S. and Fischer, R.}, title = {Die Rolle des Respirationsquotienten in der Zellkulturfermentation}, series = {Chemie Ingenieur Technik}, volume = {82}, journal = {Chemie Ingenieur Technik}, number = {9}, isbn = {Chemie Ingenieur Tec}, pages = {1505 -- 1506}, year = {2010}, language = {de} } @article{BiselliBaeckerPoghossianetal.2010, author = {Biselli, Manfred and B{\"a}cker, Matthias and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Schnitzler, Thomas and Zang, Werner and Wagner, P.}, title = {Entwicklung eines modularen festk{\"o}rperbasierten Sensorsystems f{\"u}r die {\"U}berwachung von Zellkulturfermenationen}, series = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, journal = {Sensoren und Messsysteme 2010 [Elektronische Ressource] : Vortr{\"a}ge der 15. ITG/GMA-Fachtagung vom 18. bis 19. Mai 2010 in N{\"u}rnberg / Informationstechnische Gesellschaft im VDE (ITG); VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik (GMA)}, publisher = {VDE Verlag}, address = {Berlin}, isbn = {978-3-8007-3260-9}, pages = {688 -- 691}, year = {2010}, language = {de} } @article{ClaessenGrefenMangetal.2010, author = {Claessen, O. and Grefen, Dana and Mang, Thomas and Dikland, H. G. and Dikland, H. G. and Duin, M. van}, title = {Helle Fensterprofilmaterialien : Alterungsverhalten auf Basis von peroxidisch vernetztem EPDM}, series = {Kautschuk, Gummi, Kunststoffe : KGK}, volume = {63}, journal = {Kautschuk, Gummi, Kunststoffe : KGK}, number = {9}, isbn = {0948-3276}, pages = {350 -- 360}, year = {2010}, language = {de} } @article{SrivastavaSinghAggarwaletal.2010, author = {Srivastava, A. and Singh, V. and Aggarwal, P. and Schneeweiss, F. and Scherer, Ulrich W. and Friedrich, W.}, title = {Optical studies of insulating polymers for radiation dose monitoring}, series = {Indian Journal of Pure \& Applied Physics}, volume = {48}, journal = {Indian Journal of Pure \& Applied Physics}, number = {11}, isbn = {0019-5596}, pages = {782 -- 786}, year = {2010}, language = {en} } @article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @misc{SiegertMussmannO'Connelletal.2010, author = {Siegert, Petra and Mussmann, Nina and O'Connell, Timothy and Maurer, Karl-Heinz}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patent- und Markenamt / WIPO}, address = {M{\"u}nchen / Genf}, pages = {1 -- 30}, year = {2010}, language = {de} } @misc{SiegertBaumstarkKluinetal.2010, author = {Siegert, Petra and Baumstark, Rebecca and Kluin, Cornelia and O'Connell, Timothy and Maurer, Karl-Heinz and Hellmuth, Hendrik}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patent- und Markenamt}, address = {M{\"u}nchen}, pages = {1 -- 30}, year = {2010}, language = {de} } @misc{SiegertSpitzMaurer2010, author = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz}, title = {Neue Proteasen und Mittel enthaltend diese Proteasen [Offenlegungsschrift]}, publisher = {Deutsches Patentamt / WIPO}, address = {M{\"u}nchen / Genf}, pages = {1 -- 31}, year = {2010}, language = {de} } @misc{SiegertSpitzMaurer2010, author = {Siegert, Petra and Spitz, Astrid and Maurer, Karl-Heinz}, title = {Wasch- und Reinigungsmittel enthaltend Proteasen aus Bacillus pumilus [Offenlegungsschrift]}, publisher = {Deutsches Patentamt / Europ{\"a}isches Patentamt / WIPO}, address = {M{\"u}nchen / Den Hague / Genf}, pages = {1 -- 20}, year = {2010}, language = {de} } @misc{O'ConnellSiegertEversetal.2010, author = {O'Connell, Timothy and Siegert, Petra and Evers, Stefan and Bongaerts, Johannes and Weber, Thomas and Maurer, Karl-Heinz and Bessler, Cornelius}, title = {Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft [Offenlegungsschrift]}, publisher = {Deutsches Patentamt}, address = {M{\"u}nchen}, pages = {1 -- 34}, year = {2010}, language = {de} } @incollection{SeiblerSchwenk2010, author = {Seibler, Jost and Schwenk, Frieder}, title = {Transgenic RNAi Applications in the Mouse}, series = {Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition}, booktitle = {Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {978-0-12-384880-2}, pages = {367 -- 386}, year = {2010}, language = {en} }