@article{AboulnagaZouSelmeretal.2018, author = {Aboulnaga, Elhussiny A. and Zou, Huibin and Selmer, Thorsten and Xian, Mo}, title = {Development of a plasmid-based, tunable, tolC-derived expression system for application in Cupriavidus necator H16}, series = {Journal of Biotechnology}, volume = {274}, journal = {Journal of Biotechnology}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0168-1656}, doi = {10.1016/j.jbiotec.2018.03.007}, pages = {15 -- 27}, year = {2018}, abstract = {Cupriavidus necator H16 gains increasing attention in microbial research and biotechnological application due to its diverse metabolic features. Here we present a tightly controlled gene expression system for C. necator including the pBBR1-vector that contains hybrid promoters originating from C. necator native tolC-promoter in combination with a synthetic tetO-operator. The expression of the reporter gene from these plasmids relies on the addition of the exogenous inducer doxycycline (dc). The novel expression system offers a combination of advantageous features as; (i) high and dose-dependent recombinant protein production, (ii) tight control with a high dynamic range (On/Off ratio), which makes it applicable for harmful pathways or for toxic protein production, (iii) comparable cheap inducer (doxycycline, dc), (iv) effective at low inducer concentration, that makes it useful for large scale application, (v) rapid, diffusion controlled induction, and (vi) the inducer does not interfere within the cell metabolism. As applications of the expression system in C. necator H16, the growth ability on glycerol was enhanced by constitutively expressing the E. coli glpk gene-encoding for glycerol kinase. Likewise, we used the system to overcome the expression toxicity of mevalonate pathway in C. necator H16. With this system, the mevalonate-genes were successfully introduced in the host and the recombinant strains could produce about 200 mg/l mevalonate.}, language = {en} } @inproceedings{RichterBraunsteinStaeudleetal.2018, author = {Richter, Charlotte and Braunstein, Bjoern and St{\"a}udle, Benjamin and Attias, Julia and Suess, Alexander and Weber, T. and Rittweger, Joern and Green, David A. and Albracht, Kirsten}, title = {In vivo fascicle length of the gastrocnemius muscle during walking in simulated martian gravity using two different body weight support devices}, series = {23rd Annual Congress of the European College of Sport Science, Dublin, Irland}, booktitle = {23rd Annual Congress of the European College of Sport Science, Dublin, Irland}, year = {2018}, language = {en} } @inproceedings{FingerBraunBil2018, author = {Finger, Felix and Braun, Carsten and Bil, Cees}, title = {An Initial Sizing Methodology for Hybrid-Electric Light Aircraft}, series = {AIAA AVIATION Forum 2018 Aviation Technology, Integration, and Operations Conference, Atlanta, Georgia, June 25 - 29, 2018}, booktitle = {AIAA AVIATION Forum 2018 Aviation Technology, Integration, and Operations Conference, Atlanta, Georgia, June 25 - 29, 2018}, doi = {10.2514/6.2018-4229}, year = {2018}, language = {en} } @incollection{Fabo2018, author = {Fabo, Sabine}, title = {Eine dekonstruktive Erinnerungsarbeit}, series = {Doris Frohnapfel, bewaffnete G{\"o}tter = Doris Frohnapfel, armed gods}, booktitle = {Doris Frohnapfel, bewaffnete G{\"o}tter = Doris Frohnapfel, armed gods}, publisher = {RpB Richter-Br{\"u}ckner}, address = {K{\"o}ln}, isbn = {978-3-9817241-2-7}, pages = {31 -- 35}, year = {2018}, language = {de} } @techreport{FischedickSchoofHebeletal.2018, type = {Working Paper}, author = {Fischedick, Manfred and Schoof, Ren{\´e} and Hebel, Christoph and Merkens, Torsten}, title = {Sektorenkopplung als Herausforderung und Chance f{\"u}r das Energieland NRW : Handlungsoptionen und Ergebnispapier der Expertengruppe AG 4 „Sektoren- kopplung" im Netzwerk Netze und Speicher der EnergieAgentur.NRW im Auftrag des Landes Nordrhein-Westfalen / EnergieAgentur.NRW GmbH}, pages = {68 Seiten}, year = {2018}, language = {de} }