@book{SchoeningPoghossian2018, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {Label-free biosensing: advanced materials, devices and applications}, publisher = {Springer}, address = {Cham}, isbn = {978-3-319-75219-8}, pages = {xii, 480 Seiten ; Illustrationen, Diagramme}, year = {2018}, language = {en} } @incollection{SchoeningPoghossian2018, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {Enzyme und Biosensorik}, series = {Einf{\"u}hrung in die Enzymtechnologie}, booktitle = {Einf{\"u}hrung in die Enzymtechnologie}, publisher = {Springer Spektrum}, address = {Berlin}, isbn = {978-3-662-57619-9}, doi = {10.1007/978-3-662-57619-9_18}, pages = {323 -- 347}, year = {2018}, abstract = {Enzymbasierte Biosensoren finden seit mehr als f{\"u}nf Jahrzehnten einen prosperierenden Wachstumsmarkt und werden zunehmend auch in biotechnologischen Prozessen eingesetzt. In diesem Kapitel werden, ausgehend vom Sensorbegriff und typischen Kenngr{\"o}ßen f{\"u}r Biosensoren (Abschn. 18.1), elektrochemische Enzym-Biosensoren vorgestellt und deren typischen Einsatzgebiete diskutiert (Abschn. 18.2). Ein Blick {\"u}ber den „Tellerrand" hinaus zeigt alternative Transduktorprinzipien (Abschn. 18.3) und f{\"u}hrt abschließend in aktuelle Forschungstrends ein (Abschn. 18.4).}, language = {de} } @incollection{SchoeningPoghossianGluecketal.2014, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak and Gl{\"u}ck, Olaf and Thust, Marion}, title = {Electrochemical methods for the determination of chemical variables in aqueous media}, series = {Measurement, instrumentation, and sensors handbook / ed. by John G. Webster [u.a.] Vol. 2 : Electromagnetic, optical, radiation, chemical, and biomedical measurement}, booktitle = {Measurement, instrumentation, and sensors handbook / ed. by John G. Webster [u.a.] Vol. 2 : Electromagnetic, optical, radiation, chemical, and biomedical measurement}, publisher = {CRC Pr.}, address = {Boca Raton, Fla.}, isbn = {978-1-4398-4891-3}, pages = {55-1 -- 55-54}, year = {2014}, language = {en} } @article{SchoeningPoghossianSchultze2003, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak and Schultze, Joachim W.}, title = {Measuring seven parameters by two ISFET modules in a microcell set-up}, series = {Int. Journal of Computational Engineering Science. 4 (2003), H. 2}, journal = {Int. Journal of Computational Engineering Science. 4 (2003), H. 2}, isbn = {1465-8763}, pages = {257 -- 260}, year = {2003}, language = {en} } @article{SchoeningPoghossianYoshinobuetal.2001, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak and Yoshinobu, Tatsuo and L{\"u}th, Hans}, title = {Semiconductor-based field-effect structures for chemical sensing}, pages = {188 -- 198}, year = {2001}, language = {en} } @incollection{SchoeningWagnerPoghossianetal.2018, author = {Sch{\"o}ning, Michael Josef and Wagner, Torsten and Poghossian, Arshak and Miyamoto, K.I. and Werner, C.F. and Krause, S. and Yoshinobu, T.}, title = {Light-addressable potentiometric sensors for (bio-)chemical sensing and imaging}, series = {Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry. Vol. 7}, booktitle = {Encyclopedia of Interfacial Chemistry: Surface Science and Electrochemistry. Vol. 7}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {9780128097397}, pages = {295 -- 308}, year = {2018}, language = {en} } @article{SiqueiraWernerBaeckeretal.2009, author = {Siqueira, Jose R. and Werner, Frederik and B{\"a}cker, Matthias and Poghossian, Arshak and Zucolotto, Valtencir and Oliveira, Osvaldo N. Jr. and Sch{\"o}ning, Michael Josef}, title = {Layer-by-Layer Assembly of Carbon Nanotubes Incorporated in Light-Addressable Potentiometric Sensors}, series = {Journal of Physical Chemistry C. 113 (2009), H. 33}, journal = {Journal of Physical Chemistry C. 113 (2009), H. 33}, publisher = {American Chemical Society}, address = {Washington, DC}, isbn = {1932-7455}, pages = {14765 -- 14770}, year = {2009}, language = {en} } @article{SiqueiraAbouzarBaeckeretal.2009, author = {Siqueira, Jos{\´e} R. Jr. and Abouzar, Maryam H. and B{\"a}cker, Matthias and Zucolotto, Valtencir and Poghossian, Arshak and Oliveira, Osvaldo N. Jr. and Sch{\"o}ning, Michael Josef}, title = {Carbon nanotubes in nanostructured films: Potential application as amperometric and potentiometric field-effect (bio-)chemical sensors}, series = {physica status solidi (a) . 206 (2009), H. 3}, journal = {physica status solidi (a) . 206 (2009), H. 3}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {462 -- 467}, year = {2009}, language = {en} } @article{SiqueiraAbouzarPoghossianetal.2009, author = {Siqueira, Jos{\´e} R. Jr. and Abouzar, Maryam H. and Poghossian, Arshak and Zucolotto, Valtencir and Oliveira, Osvaldo N. Jr. and Sch{\"o}ning, Michael Josef}, title = {Penicillin biosensor based on a capacitive field-effect structure functionalized with a dendrimer/carbon nanotube multilayer}, series = {Biosensors and Bioelectronics. 25 (2009), H. 2}, journal = {Biosensors and Bioelectronics. 25 (2009), H. 2}, isbn = {0956-5663}, pages = {497 -- 501}, year = {2009}, language = {en} } @article{SiqueiraBaeckerPoghossianetal.2010, author = {Siqueira, Jos{\´e} R. Jr. and B{\"a}cker, Matthias and Poghossian, Arshak and Zucolotto, Valtencir and Oliveira, Osvaldo N. Jr. and Sch{\"o}ning, Michael Josef}, title = {Associating biosensing properties with the morphological structure of multilayers containing carbon nanotubes on field-effect devices}, series = {Physica status solidi (a). 207 (2010), H. 4}, journal = {Physica status solidi (a). 207 (2010), H. 4}, isbn = {1862-6300}, pages = {781 -- 786}, year = {2010}, language = {en} } @article{SpelthahnPoghossianSchoening2009, author = {Spelthahn, Heiko and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Self-aligned nanogaps and nanochannels via conventional photolithography and pattern-size reduction technique}, series = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, journal = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, isbn = {0013-4686}, pages = {6010 -- 6014}, year = {2009}, language = {en} } @article{TurekKeusgenPoghossianetal.2008, author = {Turek, M. and Keusgen, M. and Poghossian, Arshak and Mulchandani, A. and Wang, J. and Sch{\"o}ning, Michael Josef}, title = {Enzyme-modified electrolyte-insulator-semiconductor sensors}, series = {Journal of Contemporary Physics. 43 (2008), H. 2}, journal = {Journal of Contemporary Physics. 43 (2008), H. 2}, isbn = {1934-9378}, pages = {82 -- 85}, year = {2008}, language = {en} } @article{WagnerBegingRotteretal.2007, author = {Wagner, Torsten and Beging, Stefan and Rotter, L. and Poghossian, Arshak and Biselli, Manfred and Zang, Werner and Sch{\"o}ning, Michael Josef}, title = {Online-Messsysteme f{\"u}r die automatisierte Charakterisierung von feldeffektbasierten Biosensoren}, series = {8. Dresdner Sensor-Symposium : Sensoren f{\"u}r Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme f{\"u}r die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung f{\"u}r die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)}, journal = {8. Dresdner Sensor-Symposium : Sensoren f{\"u}r Umwelt, Klima und Sicherheit, Biosensoren und Biosysteme, Sensoren und Sensorsysteme f{\"u}r die Prozesstechnik, Trends in der Sensortechnik, Materialentwicklung f{\"u}r die Sensorik; 8. Dresdner Sensor-Symposium, 10. - 12. Dezember 2007, Dresden / Gerald Gerlach ... (Hg.)}, publisher = {TUDpress, Verl. der Wissenschaften}, address = {Dresden}, isbn = {978-3-940046-45-1}, pages = {257 -- 260}, year = {2007}, language = {de} } @article{WagnerMarisAckermannetal.2007, author = {Wagner, Torsten and Maris, Rob J. and Ackermann, Hans-Josef and Otto, Ralph and Beging, Stefan and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Handheld measurement device for field-effect sensor structures: Practical evaluation and limitations}, series = {Sensors and Actuators B: Chemical . 127 (2007), H. 1}, journal = {Sensors and Actuators B: Chemical . 127 (2007), H. 1}, isbn = {0925-4005}, pages = {217 -- 223}, year = {2007}, language = {en} } @inproceedings{WeilPoghossianSchoeningetal.2012, author = {Weil, M. and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Cherstvy, A.}, title = {Electrical monitoring of layer-by-layer adsorption of oppositely charged macromolecules by means of capacitive field-effect devices}, isbn = {978-3-9813484-2-2}, doi = {10.5162/IMCS2012/P2.5.2}, pages = {1575 -- 1578}, year = {2012}, language = {en} } @article{WeldenPoghossianVahidpouretal.2022, author = {Welden, Melanie and Poghossian, Arshak and Vahidpour, Farnoosh and Wendlandt, Tim and Keusgen, Michael and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers}, series = {Biosensors}, volume = {12}, journal = {Biosensors}, number = {1}, publisher = {MDPI}, address = {Basel}, issn = {2079-6374}, doi = {10.3390/bios12010043}, pages = {Artikel 43}, year = {2022}, abstract = {Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1-3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate.}, language = {en} } @article{WeldenSeverinsPoghossianetal.2022, author = {Welden, Melanie and Severins, Robin and Poghossian, Arshak and Wege, Christina and Bongaerts, Johannes and Siegert, Petra and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor}, series = {Chemosensors}, volume = {10}, journal = {Chemosensors}, number = {6}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors10060218}, pages = {Artikel 218}, year = {2022}, abstract = {Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte-insulator-semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin-streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage-current, capacitance-voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution.}, language = {en} } @inproceedings{WeldenSeverinsPoghossianetal.2022, author = {Welden, Melanie and Severins, Robin and Poghossian, Arshak and Wege, Christina and Siegert, Petra and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Studying the immobilization of acetoin reductase with Tobacco mosaic virus particles on capacitive field-effect sensors}, series = {2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN)}, booktitle = {2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN)}, publisher = {IEEE}, isbn = {978-1-6654-5860-3 (Online)}, doi = {10.1109/ISOEN54820.2022.9789657}, pages = {4 Seiten}, year = {2022}, abstract = {A capacitive electrolyte-insulator-semiconductor (EISCAP) biosensor modified with Tobacco mosaic virus (TMV) particles for the detection of acetoin is presented. The enzyme acetoin reductase (AR) was immobilized on the surface of the EISCAP using TMV particles as nanoscaffolds. The study focused on the optimization of the TMV-assisted AR immobilization on the Ta 2 O 5 -gate EISCAP surface. The TMV-assisted acetoin EISCAPs were electrochemically characterized by means of leakage-current, capacitance-voltage, and constant-capacitance measurements. The TMV-modified transducer surface was studied via scanning electron microscopy.}, language = {en} } @article{WendlandtKochBritzetal.2023, author = {Wendlandt, Tim and Koch, Claudia and Britz, Beate and Liedek, Anke and Schmidt, Nora and Werner, Stefan and Gleba, Yuri and Vahidpour, Farnoosh and Welden, Melanie and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System}, series = {Viruses}, volume = {9}, journal = {Viruses}, number = {15}, publisher = {MDPI}, address = {Basel}, issn = {1999-4915}, doi = {doi.org/10.3390/v15091951}, pages = {Artikel 1951}, year = {2023}, abstract = {Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.}, language = {en} } @inproceedings{WuBronderPoghossianetal.2014, author = {Wu, Chunsheng and Bronder, Thomas and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {DNA-hybridization detection using light-addressable potentiometric sensor modified with gold layer}, series = {Sensoren und Messsysteme 2014 ; Beitr{\"a}ge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in N{\"u}rnberg. (ITG-Fachbericht ; 250)}, booktitle = {Sensoren und Messsysteme 2014 ; Beitr{\"a}ge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in N{\"u}rnberg. (ITG-Fachbericht ; 250)}, publisher = {VDE-Verl.}, address = {D{\"u}sseldorf}, organization = {VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, isbn = {978-3-8007-3622-5}, pages = {1 -- 4}, year = {2014}, language = {en} }