@book{Barnat2010, author = {Barnat, Miriam}, title = {Beobachtungskommunikation}, pages = {62 S.}, year = {2010}, language = {de} } @article{OlbertzKleinertz2010, author = {Olbertz, Klaus and Kleinertz, Caroline}, title = {Urlaub, Feiertage und Krankheit - Kurzarbeit in Vollzug}, series = {Arbeit und Arbeitsrecht : AuA}, journal = {Arbeit und Arbeitsrecht : AuA}, number = {6}, publisher = {Huss Medien}, address = {Berlin}, issn = {0323-4568}, pages = {348 -- 351}, year = {2010}, language = {de} } @article{OrzadaMaderwaldPoseretal.2010, author = {Orzada, Stephan and Maderwald, Stefan and Poser, Benedikt Andreas and Bitz, Andreas and Quick, Harald H. and Ladd, Mark E.}, title = {RF excitation using time interleaved acquisition of modes (TIAMO) to address B1 inhomogeneity in high-field MRI}, series = {Magnetic Resonance in Medicine}, volume = {64}, journal = {Magnetic Resonance in Medicine}, number = {2}, publisher = {Wiley-Liss}, address = {New York}, issn = {1522-2594}, doi = {10.1002/mrm.22527}, pages = {327 -- 333}, year = {2010}, abstract = {As the field strength and, therefore, the operational frequency in MRI is increased, the wavelength approaches the size of the human head/body, resulting in wave effects, which cause signal decreases and dropouts. Several multichannel approaches have been proposed to try to tackle these problems, including RF shimming, where each element in an array is driven by its own amplifier and modulated with a certain (constant) amplitude and phase relative to the other elements, and Transmit SENSE, where spatially tailored RF pulses are used. In this article, a relatively inexpensive and easy to use imaging scheme for 7 Tesla imaging is proposed to mitigate signal voids due to B1 field inhomogeneity. Two time-interleaved images are acquired using a different excitation mode for each. By forming virtual receive elements, both images are reconstructed together using GRAPPA to achieve a more homogeneous image, with only small SNR and SAR penalty in head and body imaging at 7 Tesla.}, language = {en} } @article{KraffBitzDammannetal.2010, author = {Kraff, Oliver and Bitz, Andreas and Dammann, Philipp and Ladd, Susanne C. and Ladd, Mark E. and Quick, Harald H.}, title = {An eight-channel transmit/receive multipurpose coil for musculoskeletal MR imaging at 7 T}, series = {Medical Physics}, volume = {37}, journal = {Medical Physics}, number = {12}, publisher = {Wiley}, address = {Hoboken, NJ}, issn = {2473-4209}, doi = {10.1118/1.3517176}, pages = {6368 -- 6376}, year = {2010}, abstract = {Purpose: MRI plays a leading diagnostic role in assessing the musculoskeletal (MSK) system and is well established for most questions at clinically used field strengths (up to 3 T). However, there are still limitations in imaging early stages of cartilage degeneration, very fine tendons and ligaments, or in locating nerve lesions, for example. 7 T MRI of the knee has already received increasing attention in the current published literature, but there is a strong need to develop new radiofrequency (RF) coils to assess more regions of the MSK system. In this work, an eight-channel transmit/receive RF array was built as a multipurpose coil for imaging some of the thus far neglected regions. An extensive coil characterization protocol and first in vivo results of the human wrist, shoulder, elbow, knee, and ankle imaged at 7 T will be presented. Methods: Eight surface loop coils with a dimension ofurn:x-wiley:00942405:media:mp7176:mp7176-math-0001 were machined from FR4 circuit board material. To facilitate easy positioning, two coil clusters, each with four loop elements, were combined to one RF transmit/receive array. An overlapped and shifted arrangement of the coil elements was chosen to reduce the mutual inductance between neighboring coils. A phantom made of body-simulating liquid was used for tuning and matching on the bench. Afterward, the S-parameters were verified on a human wrist, elbow, and shoulder. For safety validation, a detailed compliance test was performed including full wave simulations of the RF field distribution and the corresponding specific absorption rate (SAR) for all joints. In vivo images of four volunteers were assessed with gradient echo and spin echo sequences modified to obtain optimal image contrast, full anatomic coverage, and the highest spatial resolution within a reasonable acquisition time. The performance of the RF coil was additionally evaluated by in vivo B1 mapping. Results: A comparison of B1 per unit power, flip angle distribution, and anatomic images showed a fairly homogeneous excitation for the smaller joints (elbow, wrist, and ankle), while for the larger joints, the shoulder and especially the knee, B1 inhomogeneities and limited penetration depth were more pronounced. However, the greater part of the shoulder joint could be imaged.In vivo images rendered very fine anatomic details such as fascicles of the median nerve and the branching of the nerve bundles. High-resolution images of cartilage, labrum, and tendons could be acquired. Additionally, turbo spin echo (TSE) and inversion recovery sequences performed very well. Conclusions: This study demonstrates that the concept of two four-channel transmit/receive RF arrays can be used as a multipurpose coil for high-resolutionin vivo MR imaging of the musculoskeletal system at 7 T. Not only gradient echo but also typical clinical and SAR-intensive sequences such as STIR and TSE performed well. Imaging of small structures and peripheral nerves could in particular benefit from this technique.}, language = {en} } @article{SchlamannVoigtMaderwaldetal.2010, author = {Schlamann, Marc and Voigt, Melanie A. and Maderwald, Stefan and Bitz, Andreas and Kraff, Oliver and Ladd, Susanne C. and Ladd, Mark E. and Forsting, Michael and Wilhelm, Hans}, title = {Exposure to high-field MRI does not affect cognitive function}, series = {Journal of Magnetic Resonance Imaging}, volume = {31}, journal = {Journal of Magnetic Resonance Imaging}, number = {5}, publisher = {Wiley-Liss}, address = {New York}, issn = {1522-2586}, doi = {10.1002/jmri.22065}, pages = {1061 -- 1066}, year = {2010}, abstract = {Purpose To assess potential cognitive deficits under the influence of static magnetic fields at various field strengths some studies already exist. These studies were not focused on attention as the most vulnerable cognitive function. Additionally, mostly no magnetic resonance imaging (MRI) sequences were performed. Materials and Methods In all, 25 right-handed men were enrolled in this study. All subjects underwent one MRI examination of 63 minutes at 1.5 T and one at 7 T within an interval of 10 to 30 days. The order of the examinations was randomized. Subjects were referred to six standardized neuropsychological tests strictly focused on attention immediately before and after each MRI examination. Differences in neuropsychological variables between the timepoints before and after each MRI examination were assessed and P-values were calculated Results Only six subtests revealed significant differences between pre- and post-MRI. In these tests the subjects achieved better results in post-MRI testing than in pre-MRI testing (P = 0.013-0.032). The other tests revealed no significant results. Conclusion The improvement in post-MRI testing is only explicable as a result of learning effects. MRI examinations, even in ultrahigh-field scanners, do not seem to have any persisting influence on the attention networks of human cognition immediately after exposure.}, language = {en} } @article{SchlamannYoonMaderwaldetal.2010, author = {Schlamann, Marc and Yoon, Min-Suk and Maderwald, Stefan and Pietrzyk, Thomas and Bitz, Andreas and Gerwig, Marcus and Forsting, Michael and Ladd, Susanne C. and Ladd, Mark E. and Kastrup, Oliver}, title = {Short term effects of magnetic resonance imaging on excitability of the motor cortex at 1.5T and 7T}, series = {Academic Radiology}, volume = {17}, journal = {Academic Radiology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1076-6332}, doi = {10.1016/j.acra.2009.10.004}, pages = {277 -- 281}, year = {2010}, abstract = {Rationale and Objectives The increasing spread of high-field and ultra-high-field magnetic resonance imaging (MRI) scanners has encouraged new discussion of the safety aspects of MRI. Few studies have been published on possible cognitive effects of MRI examinations. The aim of this study was to examine whether changes are measurable after MRI examinations at 1.5 and 7 T by means of transcranial magnetic stimulation (TMS). Materials and Methods TMS was performed in 12 healthy, right-handed male volunteers. First the individual motor threshold was specified, and then the cortical silent period (SP) was measured. Subsequently, the volunteers were exposed to the 1.5-T MRI scanner for 63 minutes using standard sequences. The MRI examination was immediately followed by another TMS session. Fifteen minutes later, TMS was repeated. Four weeks later, the complete setting was repeated using a 7-T scanner. Control conditions included lying in the 1.5-T scanner for 63 minutes without scanning and lying in a separate room for 63 minutes. TMS was performed in the same way in each case. For statistical analysis, Wilcoxon's rank test was performed. Results Immediately after MRI exposure, the SP was highly significantly prolonged in all 12 subjects at 1.5 and 7 T. The motor threshold was significantly increased. Fifteen minutes after the examination, the measured value tended toward normal again. Control conditions revealed no significant differences. Conclusion MRI examinations lead to a transient and highly significant alteration in cortical excitability. This effect does not seem to depend on the strength of the static magnetic field.}, language = {en} } @inproceedings{AbelBoninAlbrachtetal.2010, author = {Abel, Thomas and Bonin, Dominik and Albracht, Kirsten and Zeller, Sebastian and Br{\"u}ggemann, Gert-Peter and Burkett, Brendan and Str{\"u}der, Heiko K.}, title = {Kinematic profile of the elite handcyclist}, series = {28th International Conference on Biomechanics in Sports, Marquette, Michigan, USA, July 19 - 23, 2010}, booktitle = {28th International Conference on Biomechanics in Sports, Marquette, Michigan, USA, July 19 - 23, 2010}, issn = {1999-4168}, pages = {140 -- 141}, year = {2010}, language = {en} } @phdthesis{Albracht2010, author = {Albracht, Kirsten}, title = {Influence of mechanical properties of the leg extensor muscletendon units on running economy}, publisher = {Deutsche Sporthochschule K{\"o}ln}, address = {K{\"o}ln}, pages = {X, 1221 Bl. : graph. Darst.}, year = {2010}, language = {en} } @article{BialonskiHorstmannLehnertz2010, author = {Bialonski, Stephan and Horstmann, Marie-Therese and Lehnertz, Klaus}, title = {From brain to earth and climate systems: Small-world interaction networks or not?}, series = {Chaos: An Interdisciplinary Journal of Nonlinear Science}, volume = {20}, journal = {Chaos: An Interdisciplinary Journal of Nonlinear Science}, number = {1}, publisher = {AIP Publishing}, address = {Melville, NY}, issn = {1089-7682}, doi = {10.1063/1.3360561}, pages = {013134}, year = {2010}, abstract = {We consider recent reports on small-world topologies of interaction networks derived from the dynamics of spatially extended systems that are investigated in diverse scientific fields such as neurosciences, geophysics, or meteorology. With numerical simulations that mimic typical experimental situations, we have identified an important constraint when characterizing such networks: indications of a small-world topology can be expected solely due to the spatial sampling of the system along with the commonly used time series analysis based approaches to network characterization.}, language = {en} } @article{HorstmannBialonskiNoenningetal.2010, author = {Horstmann, Marie-Therese and Bialonski, Stephan and Noenning, Nina and Mai, Heinke and Prusseit, Jens and Wellmer, J{\"o}rg and Hinrichs, Hermann and Lehnertz, Klaus}, title = {State dependent properties of epileptic brain networks: Comparative graph-theoretical analyses of simultaneously recorded EEG and MEG}, series = {Clinical Neurophysiology}, volume = {121}, journal = {Clinical Neurophysiology}, number = {2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1388-2457}, doi = {10.1016/j.clinph.2009.10.013}, pages = {172 -- 185}, year = {2010}, abstract = {Objective To investigate whether functional brain networks of epilepsy patients treated with antiepileptic medication differ from networks of healthy controls even during the seizure-free interval. Methods We applied different rules to construct binary and weighted networks from EEG and MEG data recorded under a resting-state eyes-open and eyes-closed condition from 21 epilepsy patients and 23 healthy controls. The average shortest path length and the clustering coefficient served as global statistical network characteristics. Results Independent on the behavioral condition, epileptic brains exhibited a more regular functional network structure. Similarly, the eyes-closed condition was characterized by a more regular functional network structure in both groups. The amount of network reorganization due to behavioral state changes was similar in both groups. Consistent findings could be achieved for networks derived from EEG but hardly from MEG recordings, and network construction rules had a rather strong impact on our findings. Conclusions Despite the locality of the investigated processes epileptic brain networks differ in their global characteristics from non-epileptic brain networks. Further methodological developments are necessary to improve the characterization of disturbed and normal functional networks. Significance An increased regularity and a diminished modulation capability appear characteristic of epileptic brain networks.}, language = {en} } @incollection{LehnertzBialonskiHorstmannetal.2010, author = {Lehnertz, Klaus and Bialonski, Stephan and Horstmann, Marie-Therese and Krug, Dieter and Rothkegel, Alexander and Staniek, Matth{\"a}us and Wagner, Tobias}, title = {Epilepsy}, series = {Reviews of Nonlinear Dynamics and Complexity, Volume 2}, booktitle = {Reviews of Nonlinear Dynamics and Complexity, Volume 2}, publisher = {Wiley-VCH}, isbn = {9783527628001}, doi = {10.1002/9783527628001.ch5}, pages = {159 -- 200}, year = {2010}, language = {en} } @article{KnackstedtEggertGraeweetal.2010, author = {Knackstedt, Ralf and Eggert, Mathias and Gr{\"a}we, Lena and Spittka, Jan}, title = {Forschungsportal f{\"u}r Rechtsinformatik und Informationsrecht - Weg zu einer disziplinen{\"u}bergreifenden Forschungs{\"u}bersicht}, series = {MMR - Multimedia und Recht}, journal = {MMR - Multimedia und Recht}, number = {8}, publisher = {Beck}, address = {M{\"u}nchen}, issn = {1434-596X}, pages = {528 -- 533}, year = {2010}, abstract = {Die Entwicklungen der Rechtsinformatik und des Informationsrechts zeigen, dass diese Disziplinen aktuell vor der Herausforderung stehen, eine interdisziplin{\"a}re Zusammenarbeit zwischen ihnen und anderen Disziplinen zu etablieren. Unterschiedliche Publikationskulturen erschweren die Erreichung dieses Ziels. Forschungsportale stellen themenspezifische, internetbasierte Verzeichnisse dar, die bereits vorhandene Informationen strukturiert zug{\"a}nglich machen. Sie k{\"o}nnen die Beziehungen zwischen den Disziplinen f{\"o}rdern, indem sie bereits erzielte Arbeitsergebnisse disziplinen{\"u}bergreifend bekannt machen und dadurch dazu beitragen, Synergiepotenziale und m{\"o}gliche Kooperationspartner zu identifizieren.}, language = {de} } @inproceedings{BeckerEggertKnackstedt2010, author = {Becker, J{\"o}rg and Eggert, Mathias and Knackstedt, Ralf}, title = {Der Beitrag der Referenzmodellierung zur Compliance im Reporting des Finanzsektors}, series = {Business Intelligence Operational Structures: Towards the Design of a Reference Process Map}, booktitle = {Business Intelligence Operational Structures: Towards the Design of a Reference Process Map}, pages = {11 -- 18}, year = {2010}, language = {de} } @article{KrollLudwigs2010, author = {Kroll-Ludwigs, Kathrin}, title = {Die Zukunft des verbrauchersch{\"u}tzenden Widerrufsrechts in Europa}, series = {Zeitschrift f{\"u}r europ{\"a}isches Privatrecht : ZEuP}, volume = {18}, journal = {Zeitschrift f{\"u}r europ{\"a}isches Privatrecht : ZEuP}, number = {3}, publisher = {Beck}, address = {M{\"u}nchen}, issn = {0943-3929}, pages = {509 -- 534}, year = {2010}, abstract = {Das verbrauchersch{\"u}tzende Widerrufsrecht ist in die Jahre gekommen. Als Ergebnis der zunehmenden Rechtszersplitterung im europ{\"a}ischen Richtlinienrecht hat sich eine un{\"u}berschaubare Bandbreite an Ausgestaltungsformen in den mitgliedstaatlichen Rechtssystemen herausgebildet. Effektivit{\"a}tseinbußen, Wettbewerbsverzerrungen und ein hohes Maß an Rechtsunsicherheit sind die Folgen. Zur{\"u}ckf{\"u}hren l{\"a}sst sich die kostenintensive Fragmentierung des Verbrauchervertragsrechts auf die bisherige Regelungspolitik der Mindestharmonisierung, die es den Mitgliedstaaten erlaubt, von dem durch die Richtlinien gesetzten Mindeststandard durch „{\"u}berschießende Umsetzung" abzuweichen. Der Unionsgesetzgeber versucht dem vermehrt durch einen Prozess der Vollharmonisierung zu begegnen: Neben Reformen der Richtlinie {\"u}ber Verbraucherkredite (VerbrKrRL) sowie der Timesharing-Richtlinie (TimesharingRL), liegt seit dem 8. Oktober 2008 nunmehr auch der Entwurf einer Rahmenrichtlinie {\"u}ber die Rechte der Verbraucher (VRRL-E) vor. Danach sollen die bisherigen Richtlinien {\"u}ber Haust{\"u}rgesch{\"a}fte (Haust{\"u}rgesch{\"a}fteRL), Fernabsatzvertr{\"a}ge (FARL), missbr{\"a}uchliche Klauseln sowie Verbrauchsg{\"u}terk{\"a}ufe vereinigt werden. F{\"u}r das verbrauchersch{\"u}tzende Widerrufsrecht ist damit zum einen die Herausbildung einheitlicher Kernelemente verbunden, zum anderen tritt es in Konkurrenz zu umfassenden Informationspflichten, die ebenfalls den Schutz des Verbrauchers vor dem un{\"u}berlegten Abschluss riskanter bzw. nachteiliger Vertr{\"a}ge zum Ziel haben. Angesichts dieser Entwicklung stellt sich im Folgenden die Frage nach der Zukunft des unionsrechtlichen Widerrufsrechts. Von besonderer Bedeutung ist dabei die Kl{\"a}rung des Verh{\"a}ltnisses zu den verbrauchersch{\"u}tzenden Informationspflichten. Dazu bedarf es zun{\"a}chst der Herausarbeitung einheitlicher Kernelemente des Widerrufsrechts im vollharmonisierten Richtlinienrecht (II.) sowie einer Systematisierung der sekund{\"a}rrechtlichen Informationspflichten im Anschluss eine kritische Gegen{\"u}berstellung der Vor- und Nachteile beider Schutzinstrumente erfolgen kann (IV.).}, language = {de} } @article{NabilPetersonSchmidtetal.2010, author = {Nabil, F. and Peterson, Leif Arne and Schmidt, A. and Geerdes, D.}, title = {Die Holzkuppel des Neuen Palais in Potsdam}, series = {Bauingenieur}, volume = {85}, journal = {Bauingenieur}, number = {8}, publisher = {Springer-VDI-Verlag}, address = {D{\"u}sseldorf}, issn = {0005-6650}, pages = {324 -- 330}, year = {2010}, language = {de} } @misc{SiekerTippkoetterUlberetal.2010, author = {Sieker, T. and Tippk{\"o}tter, Nils and Ulber, Roland and Bart, H.-J. and Heinzle, E.}, title = {Gr{\"u}ne Bioraffinerie: Ganzheitliche Nutzung von Grassilage f{\"u}r die Herstellung von Grund-und Feinchemikalien}, series = {Chemie Ingenieur Technik}, volume = {82}, journal = {Chemie Ingenieur Technik}, number = {9}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0009-286X}, doi = {10.1002/cite.201050321}, pages = {1564}, year = {2010}, abstract = {Gras hat ein hohes Potenzial als nachwachsender Rohstoff. Bei ungeeigneter Lagerung verderben Gr{\"a}ser allerdings innerhalb weniger Tage. Dieser Nachteil kann durch die Silierung des Grasschnittes behoben werden. Eines der wichtigsten in der Silage enthaltenen Produkte ist die Milchs{\"a}ure. Um diese f{\"u}r weitere Aufarbeitungsschritte zug{\"a}nglich zu machen, wird aus der Silage ein Presssaft hergestellt. Die Milchs{\"a}ure wird aus einem Silagepresssaft mittels Extraktion durch ionische Fl{\"u}ssigkeiten isoliert. Dabei wird zum einen eine reine Milchs{\"a}ure hergestellt, die z. B. f{\"u}r die Herstellung von Polymilchs{\"a}ure genutzt werden kann. Zum anderen wird ein weniger aufgereinigter Extrakt gewonnen, der f{\"u}r die fermentative Produktion von L-Lysin und 1,2-Propandiol genutzt werden soll. Im Rahmen des Projekts erfolgt die gentechnische Optimierung von Corynebacterium glutamicum f{\"u}r die Umsetzung von Milchs{\"a}urezu L-Lysin. Die im nach der Pressung verbleibenden Presskuchen enthaltenen Grasfasern bestehen zu einem großen Teil aus Polysacchariden. Diese werden hydrolysiert und die dabei freigesetzten Zucker zu Grundchemikalien wie Ethanol oder Itakons{\"a}ure fermentiert. Im Rahmen einer vollst{\"a}ndigen Nutzung der Silage wird das Raffinat aus der Milchs{\"a}ureextraktion als Mediumsupplement in der Fermentation eingesetzt, was die Zugabe weiterer Medienbestandteile {\"u}berfl{\"u}ssig macht. Die R{\"u}ckst{\"a}nde der Hydrolysen und Fermentationen sollen dar{\"u}berhinaus f{\"u}r die Herstellung von Biogas genutzt werden.}, language = {de} } @misc{TippkoetterMaurerPasteuretal.2010, author = {Tippk{\"o}tter, Nils and Maurer, S. and Pasteur, A. and Kampeis, P. and Ulber, Roland}, title = {Hochgradient-Magnetseparation von Fermentationsprodukten-FEM Simulation der Filtermatrix}, series = {Chemie Ingenieur Technik}, volume = {82}, journal = {Chemie Ingenieur Technik}, number = {9}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {0009-286X}, doi = {10.1002/cite.201050217}, pages = {1361}, year = {2010}, abstract = {Durch den Einsatz magnetisierbarer Partikel lassen sich Stoffwechselprodukte direkt und selektiv aus feststoffreichen Fermentationssuspensionen abtrennen. Im Gegensatz zu klassischen Adsorbermaterialien k{\"o}nnen magnetisierbare Partikel mit sehr geringen Durchmessern verwendet werden. Zur deren Abtrennung ist jedoch ein hoher Magnetfeldgradient notwendig. Dieser wird in der Regel durch in der Trennkammer bzw. dem Magnetfeld eingebrachte magnetisierbare Dr{\"a}hte realisiert. Bei der Auslegung der Drahtgitter ist ein Kompromiss zwischen Abtrennrate und Durchl{\"a}ssigkeit n{\"o}tig. Die Ausrichtung der Dr{\"a}hte in Relation zum Magnetfeld, deren Abstand sowie die geometrische Anordnung k{\"o}nnen hierbei variiert werden. Zum Verst{\"a}ndnis der Einfl{\"u}sse auf das sich ausbildende Magnetfeld und die Fluiddynamik wurden Simulationen mit der Finite-Elemente-Methode durchgef{\"u}hrt und experimentell {\"u}berpr{\"u}ft. Hierf{\"u}r wurden die Dr{\"a}hte unter Variation von Anzahl, Richtung und Anordnung in den Hochgradient-Magnetseparator eingebracht. Erste Verifizierungen der Simulationen zeigen, dass die in Magnetfeldrichtung ausgerichteten Dr{\"a}hte (x-Achse) {\"u}ber die geringste Partikelr{\"u}ckhaltef{\"a}higkeit verf{\"u}gen. Die Dr{\"a}hte der y- und z-Achse halten den gr{\"o}ßten Anteil der Magnetpartikel zur{\"u}ck, wobei die Dr{\"a}hte in y-Richtung den h{\"o}chsten Feldgradienten ausbilden. Des Weiteren konnte gezeigt werden, dass eine rhomboedrische Drahtanordnung der kubischen vorzuziehen ist.}, language = {de} } @article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @incollection{ReimerBraunWellmeretal.2010, author = {Reimer, Lars and Braun, Carsten and Wellmer, Georg and Behr, Marek and Ballmann, Josef}, title = {Development of a modular method for computational aero-structural analysis of aircraft}, series = {Summary of flow modulation and fluid-structure interaction findings. Results of the Collaborative Research Center SFB 401 at the RWTH Aachen University, Aachen, Germany, 1997-2008 / ed.: Wolfgang Schr{\"o}der. Notes on numerical fluid mechanics and multidisciplinary design. Vol. 109}, booktitle = {Summary of flow modulation and fluid-structure interaction findings. Results of the Collaborative Research Center SFB 401 at the RWTH Aachen University, Aachen, Germany, 1997-2008 / ed.: Wolfgang Schr{\"o}der. Notes on numerical fluid mechanics and multidisciplinary design. Vol. 109}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-642-04087-0}, pages = {205 -- 238}, year = {2010}, language = {en} } @inproceedings{AlexopoulosHoffschmidtRauetal.2010, author = {Alexopoulos, Spiros and Hoffschmidt, Bernhard and Rau, Christoph and Schmitz, M. and Schwarzb{\"o}zl, P. and Pomp, Stefan}, title = {Simulation results for a hybridized operation of a gas turbine or a burner for a small solar tower power plant}, series = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, booktitle = {SolarPACES 2010 : the CSP Conference: electricity, fuels and clean water from concentrated solar energy ; 21 to 24 September 2010, Perpignan, France}, publisher = {Soc. OSC}, address = {Saint Maur}, pages = {82 -- 83}, year = {2010}, language = {en} }