@article{AlKaidyTippkoetter2016,
  author    = {Al-Kaidy, Huschyar and Tippk{\"o}tter, Nils},
  title     = {Superparamagnetic hydrophobic particles as shell material for digital microfluidic droplets and proof-of-principle reaction assessments with immobilized laccase},
  series = {Engineering in Life Sciences},
  volume    = {16},
  journal   = {Engineering in Life Sciences},
  number    = {3},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  doi       = {10.1002/elsc.201400124},
  pages     = {222 -- 230},
  year      = {2016},
  abstract  = {In the field of biotechnology and molecular biology, the use of small liquid volumes has significant advantages. In particular, screening and optimization runs with acceptable amounts of expensive and hardly available catalysts, reagents, or biomolecules are feasible with microfluidic technologies. The presented new microfluidic system is based on the inclusion of small liquid volumes by a protective shell of magnetizable microparticles. Hereby, discrete aqueous microreactor drops with volumes of 1-30 μL can be formed on a simple planar surface. A digital movement and manipulation of the microreactor is performed by overlapping magnetic forces. The magnetic forces are generated by an electrical coil matrix positioned below a glass plate. With the new platform technology, several discrete reaction compartments can be moved simultaneously on one surface. Due to the magnetic fields, the reactors can even be merged to initiate reactions by mixing or positioned above surface-immobilized catalysts and then opened by magnetic force. Comparative synthesis routes of the magnetizable shell particles and superhydrophobic glass slides including their performance and stability with the reaction platform are described. The influence of diffusive mass transport during the catalyzed reaction is discussed by evaluation finite element model of the microreactor. Furthermore, a first model dye reaction of the enzyme laccase has been established.},
  language  = {en}
}
@article{AlKaidyDuweHusteretal.2015,
  author    = {Al-Kaidy, Huschyar and Duwe, Anna and Huster, Manuel and Muffler, Kai and Schlegel, Christin and Tim, Sieker and Stadtm{\"u}ller, Ralf and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Biotechnology and bioprocess engineering - from the first ullmann's article to recent trends},
  series = {ChemBioEng Reviews},
  volume    = {2},
  journal   = {ChemBioEng Reviews},
  number    = {3},
  publisher = {Wiley},
  address   = {Weinheim},
  doi       = {10.1002/cben.201500008},
  pages     = {175 -- 184},
  year      = {2015},
  abstract  = {For several thousand years, biotechnology and its associated technical processes have had a great impact on the development of mankind. Based on empirical methods, in particular for the production of foodstuffs and daily commodities, these disciplines have become one of the most innovative future issues. Due to the increasing detailed understanding of cellular processes, production strains can now be optimized. In combination with modern bioprocesses, a variety of bulk and fine chemicals as well as pharmaceuticals can be produced efficiently. In this article, some of the current trends in biotechnology are discussed.},
  language  = {en}
}
@article{ThielMufflerTippkoetteretal.2015,
  author    = {Thiel, Alexander and Muffler, Kai and Tippk{\"o}tter, Nils and Suck, Kirstin and Sohling, Ulrich and Hruschka, Steffen M. and Ulber, Roland},
  title     = {A novel integrated downstream processing approach to recover sinapic acid, phytic acid and proteins from rapeseed meal},
  series = {Journal of Chemical Technology and Biotechnology},
  volume    = {90},
  journal   = {Journal of Chemical Technology and Biotechnology},
  number    = {11},
  publisher = {Wiley},
  address   = {Weinheim},
  doi       = {10.1002/jctb.4664},
  pages     = {1999 -- 2006},
  year      = {2015},
  abstract  = {BACKGROUND Currently, several techniques exist for the downstream processing of protein, phytic acid and sinapic acid from rapeseed and rapeseed meal, but no technique has been developed to separate all of the components in one process. In this work, two new downstream processing strategies focusing on recovering sinapic acid, phytic acid and protein from rapeseed meal were established. RESULTS The sinapic acid content was enhanced by a factor of 4.5 with one method and 5.1 with the other. The isolation of sinapic acid was accomplished using a zeolite-based adsorbent with high adsorptive and optimal desorption characteristics. Phytic acid was isolated using the anion-exchange resin Purolite A200®. In addition, the processes resulted in two separated protein fractions. The ratios of globulin and albumin ratio to the total protein were 59.2\% and 40.1\%, respectively. The steps were then combined in two different ways: (a) a 'sequential process' using the zeolite and A200 in batch processes; and (b) a 'parallel process' using only A200 in a chromatographic system to separate all of the compounds. CONCLUSIONS It can be concluded that isolation of all three components was possible in both processes. These could enhance the added value of current processes using rapeseed meal as a protein source. © 2015 Society of Chemical Industry},
  language  = {en}
}
@article{SchumannRoginSchneideretal.2015,
  author    = {Schumann, Christiane and Rogin, Sabine and Schneider, Horst and Tippk{\"o}tter, Nils and Oster, J{\"u}rgen and Kampeis, Percy},
  title     = {Simultane Atline-Quantifizierung von Magnetpartikeln und Mikroorganismen bei einer HGMS-Filtration},
  series = {Chemie Ingenieur Technik},
  volume    = {87},
  journal   = {Chemie Ingenieur Technik},
  number    = {1-2},
  doi       = {10.1002/cite.201300158},
  pages     = {137 -- 149},
  year      = {2015},
  abstract  = {Es wird eine neue Atline-Messmethode vorgestellt, mit der w{\"a}hrend einer Hochgradienten-Magnetseparation (HGMS)-Filtration eine simultane Quantifizierung von Magnetpartikeln und Mikroorganismen im Filtrat vorgenommen werden kann. Dabei gelingt die Quantifizierung signifikant besser als mit bisher verwendeten Messmethoden. Mit dieser Methode ist es m{\"o}glich, die Trennleistung einer HGMS-Filtration zu bestimmen und einen Filterdurchbruch durch Konzentrationsanstiege im Bereich einiger µg L-1 von Magnetpartikeln im Filtrat fr{\"u}hzeitig zu detektieren, ohne dass nennenswerte Partikelmengen verloren gehen.},
  language  = {de}
}
@article{ThielMufflerTippkoetteretal.2015,
  author    = {Thiel, Alexander and Muffler, Kai and Tippk{\"o}tter, Nils and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland},
  title     = {Aufarbeitung von Polyphenolen aus Weizen mittels Zeolithen am Beispiel der Ferulas{\"a}ure},
  series = {Chemie IngenieurTechnik},
  volume    = {87},
  journal   = {Chemie IngenieurTechnik},
  number    = {1-2},
  publisher = {Wiley},
  address   = {Weinheim},
  doi       = {10.1002/cite.201400031},
  pages     = {128 -- 136},
  year      = {2015},
  abstract  = {Aufarbeitung von Polyphenolen aus Weizenmittels Zeolithen am Beispiel der Ferulasa¨ ureAlexander Thiel1, Kai Muffler1, Nils Tippko¨ tter1, Kirstin Suck2, Ulrich Sohling2, Friedrich Ruf3und Roland Ulber1,*DOI: 10.1002/cite.201400031Bei der Ferulasa¨ure handelt es sich um einen Wertstoff, der aus Weizen gewonnen und in der Lebensmittel- und Pharma-industrie eingesetzt werden kann. Der Einsatz von Weizen als nachwachsende Rohstoffquelle ist allerdings nur dann wirt-schaftlich durchfu¨hrbar, wenn eine Prozessintegration in die bestehenden industriellen Verfahren gewa¨hrleistet oder einedirekte Konkurrenz zur Mehl- und Sta¨rkeindustrie vermieden werden kann. In diesem Artikel wird ein Verfahren aufge-zeigt, welches hohe Ausbeuten ermo¨glicht und eine Konkurrenz zu bestehenden Verwertungspfaden vermeidet.},
  language  = {de}
}
@article{WiesenTippkoetterMuffleretal.2015,
  author    = {Wiesen, Sebastian and Tippk{\"o}tter, Nils and Muffler, Kai and Suck, Kirstin and Sohling, Ulrich and Ruf, Friedrich and Ulber, Roland},
  title     = {Adsorption of fatty acids to layered double hydroxides in aqueous systems},
  series = {Adsorption},
  volume    = {21},
  journal   = {Adsorption},
  number    = {6-7},
  publisher = {Springer},
  address   = {Berlin},
  pages     = {459 -- 466},
  year      = {2015},
  abstract  = {Due to their anion exchange characteristics, layered double hydroxides (LDHs) are suitable for the detoxification of aqueous, fatty acid containing fermentation substrates. The aim of this study is to examine the adsorption mechanism, using crude glycerol from plant oil esterification as a model system. Changes in the intercalation structure in relation to the amount of fatty acids adsorbed are monitored by X-ray diffraction and infra-red spectroscopy. Additionally, calcination of LDH is investigated in order to increase the binding capacity for fatty acids. Our data propose that, at ambient temperature, fatty acids can be bound to the hydrotalcite by adsorption or in addition by intercalation, depending on fatty acid concentration. The adsorption of fatty acids from crude glycerol shows a BET-like behavior. Above a fatty acid concentration of 3.5 g L-1, intercalation of fatty acids can be shown by the appearance of an increased interlayer spacing. This observation suggests a two phase adsorption process. Calcination of LDHs allows increasing the binding capacity for fatty acids by more than six times, mainly by reduction of structural CO32-.},
  language  = {en}
}
@article{TippkoetterDuweWiesenetal.2014,
  author    = {Tippk{\"o}tter, Nils and Duwe, Anna-Maria and Wiesen, Sebastian and Sieker, Tim and Ulber, Roland},
  title     = {Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids},
  series = {Bioresource Technology},
  volume    = {167},
  journal   = {Bioresource Technology},
  publisher = {Elsevier},
  address   = {Amsterdam},
  doi       = {10.1016/j.biortech.2014.06.052},
  pages     = {447 -- 455},
  year      = {2014},
  abstract  = {The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10\% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9\%, 17\% and 23\% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L⁻¹ are reached. However, the glucose yield decreases from 0.81 to 0.72gg⁻¹ at 17\% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23\% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49gg⁻¹. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33gg⁻¹.},
  language  = {en}
}
@article{GrafSteinhofLotzetal.2009,
  author    = {Graf, Alain-Michel and Steinhof, Rafael and Lotz, Martin and Tippk{\"o}tter, Nils and Kasper, Cornelia and Beutel, Sascha and Ulber, Roland},
  title     = {Downstream-Processing mit Membranadsorbern zur Isolierung nativer Proteinfraktionen aus Kartoffelfruchtwasser},
  series = {Chemie Ingenieur Technik},
  volume    = {81},
  journal   = {Chemie Ingenieur Technik},
  number    = {3},
  publisher = {Wiley},
  address   = {Weinheim},
  doi       = {10.1002/cite.200800139},
  pages     = {267 -- 274},
  year      = {2009},
  abstract  = {Bei der St{\"a}rkeproduktion entstehendes Kartoffelfruchtwasser besitzt mit 2 - 3 \% einen hohen Anteil an ern{\"a}hrungsphysiologisch interessanten Proteinen. Die industrielle Gewinnung dieser Proteinfracht liefert jedoch lediglich ein minderwertiges, denaturiertes Produkt. Mit Hilfe der Membranadsorber-Technologie lassen sich aus Kartoffelfruchtwasser unter milden Reaktionsbedingungen native bioaktive Proteinfraktionen gewinnen. Geeignete Trennbedingungen wurden im Labormaßstab entwickelt und in den Technikumsmaßstab {\"u}bertragen. An Anionenaustauscher-Membranadsorbern mit einer Membranfl{\"a}che von 10 000 cm2 wurde eine Patatinhaltige Fraktion (44 kDa) mit Bindungskapazit{\"a}ten von 0,37 mg/cm2 isoliert. Eine niedermolekulare Proteinfraktion mit Protease-Inhibitoren konnte durch Kationenaustauscher-Membranadsorber mit Bindungskapazit{\"a}ten von 1,00 mg/cm2 gewonnen werden. Sie ist f{\"u}r verschiedenste Applikationen in der pharmazeutischen, kosmetischen und der Nahrungsmittelindustrie interessant z. B. f{\"u}r Appetitz{\"u}gler oder muskelaufbauende Proteinpr{\"a}parate. Der Aufreinigung der nativen Proteinfraktionen durch Ultra-/Diafiltration schließt sich die Konfektionierung durch Spr{\"u}htrocknung an. Die bioanalytische Charakterisierung der Produkte belegt die Reinheit und die enzymatische Aktivit{\"a}t sowie die Abreicherung von St{\"o}rkomponenten wie Glykoalkaloide und Polyphenoloxidasen.},
  language  = {de}
}
@article{TippkoetterDeterdingUlber2008,
  author    = {Tippk{\"o}tter, Nils and Deterding, A. and Ulber, Roland},
  title     = {Determination of acetic acid in fermentation broth by gas-diffusion technique},
  series = {Engineering in Life Sciences},
  volume    = {8},
  journal   = {Engineering in Life Sciences},
  number    = {1, Special Issue: Technical Systems for the Use in Life Sciences},
  doi       = {10.1002/elsc.200820227},
  pages     = {62 -- 67},
  year      = {2008},
  abstract  = {Due to the interfering effects of acetic acid in many fermentation processes, a gas-diffusion technique was developed for the online determination of acetic acid. The measurements were accomplished with a flow diffusion analysis (FDA) unit from the TRACE Analytics GmbH, Braunschweig, Germany. The diffusion analysis is based on the UV-absorbance of acetic acid at 205 nm. The measurement was achieved by the separation of an acceptor and a carrier stream (acidified fermentation broth) using a gas permeable polytetrafluoroethylene (PTFE) membrane, whereby broth constituents that would otherwise disturb the UV-measurement of acetic acid, are held back efficiently. Merely, the fermentation by-products, e.g. formic acid, is capable of diffusing through the membrane. While formic acid can disturb the measurement, carbon dioxide does not absorb at 205 nm. The method operates with time-dependent sample enrichment. During the analysis, a small volume of the acceptor stream is stopped for a defined time interval in the acceptor chamber. During this period, the gaseous acetic acid diffuses through the membrane and is enriched in the acceptor chamber. Subsequently after the enrichment, the acceptor stream flows through a UV-detector. The intensity of the signal is proportional to the acetic acid concentration. Online measurements in bioreactors via a sterile filtration probe have been accomplished. A linear calibration in the range of 0.5-5.0 g/L acetic acid with a relative standard deviation of <5 \% was obtained. A sampling rate of 8 samples per hour was possible. The system was applied for the determination of acetic acid in E. coli fermentation broth. The instrument is easy to clean, very user-friendly and does not require any toxic or expensive reagents.},
  language  = {en}
}
@article{KapplerTanudyayaSchmittTippkoetteretal.2007,
  author    = {Kappler-Tanudyaya, Nathalie and Schmitt, Heike and Tippk{\"o}tter, Nils and Meyer, Lina and Lenzen, Sigurd and Ulber, Roland},
  title     = {Combination of biotransformation and chromatography for the isolation and purification of mannoheptulose},
  series = {Biotechnology Journal},
  volume    = {2},
  journal   = {Biotechnology Journal},
  number    = {6},
  issn      = {1860-7314},
  doi       = {10.1002/biot.200700004},
  pages     = {692 -- 699},
  year      = {2007},
  abstract  = {Mannoheptulose is a seven-carbon sugar. It is an inhibitor of glucose-induced insulin secretion due to its ability to selectively inhibit the enzyme glucokinase. An improved procedure for mannoheptulose isolation from avocados is described in this study (based upon the original method by La Forge). The study focuses on the combination of biotransformation and downstream processing (preparative chromatography) as an efficient method to produce a pure extract of mannoheptulose. The experiments were divided into two major phases. In the first phase, several methods and parameters were compared to optimize the mannoheptulose extraction with respect to efficiency and purity. In the second phase, a mass balance of mannoheptulose over the whole extraction process was undertaken to estimate the yield and efficiency of the total extraction process. The combination of biotransformation and preparative chromatography allowed the production of a pure mannoheptulose extract. In a biological test, the sugar inhibited the glucokinase enzyme activity efficiently.},
  language  = {en}
}
@incollection{Tippkoetter2016,
  author    = {Tippk{\"o}tter, Nils},
  title     = {Grundlagen der bio-chemischen Umwandlung},
  series = {Energie aus Biomasse : Grundlagen, Techniken und Verfahren},
  booktitle = {Energie aus Biomasse : Grundlagen, Techniken und Verfahren},
  editor    = {Kaltschmidt, Martin},
  edition   = {3., aktualisierte, erweiterte Auflage},
  publisher = {Springer Vieweg},
  address   = {Berlin ; Heidelberg},
  isbn      = {978-3-662-47437-2 (Print)},
  doi       = {10.1007/978-3-662-47438-9},
  pages     = {1447 -- 1500},
  year      = {2016},
  language  = {de}
}
@incollection{HahnKellyMuffleretal.2011,
  author    = {Hahn, Thomas and Kelly, Svenja and Muffler, Kai and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Extraction of lignocellulose and algae for the production of bulk and fine chemicals},
  series = {Industrial scale natural products extraction},
  booktitle = {Industrial scale natural products extraction},
  editor    = {Hans-J{\"o}rg, Bart and Pilz, Stephan},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  isbn      = {978-3-527-32504-7 (Print)},
  doi       = {10.1002/9783527635122},
  pages     = {221 -- 245},
  year      = {2011},
  language  = {en}
}
@incollection{MufflerPothSiekeretal.2011,
  author    = {Muffler, Kai and Poth, Sabastian and Sieker, Tim and Tippk{\"o}tter, Nils and Ulber, Roland and Sell, Dieter},
  title     = {Bio-feedstocks},
  series = {Comprehensive biotechnology : principles and practices in industry, agcriculture, medicine and the environment. Volume 2: Engineering fundamentals of biotechnology},
  booktitle = {Comprehensive biotechnology : principles and practices in industry, agcriculture, medicine and the environment. Volume 2: Engineering fundamentals of biotechnology},
  editor    = {Moo-Young, Murray},
  edition   = {2. edition},
  publisher = {Elsevier},
  address   = {Amsterdam},
  isbn      = {978-0-444-53352-4},
  doi       = {10.1016/B978-0-08-088504-9.00088-X},
  pages     = {93 -- 101},
  year      = {2011},
  language  = {en}
}
@incollection{MufflerTippkoetterUlber2010,
  author    = {Muffler, Kai and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Chemical feedstocks and fine chemicals from other substrates},
  series = {Handbook of hydrocarbon and lipid microbiology. Volume 4: Consequences of microbial interactions with hydrocarbons, oils and lipids. - (Springer reference)},
  booktitle = {Handbook of hydrocarbon and lipid microbiology. Volume 4: Consequences of microbial interactions with hydrocarbons, oils and lipids. - (Springer reference)},
  editor    = {Timmis, Kenneth N.},
  publisher = {Springer},
  address   = {Berlin [u.a.]},
  isbn      = {978-3-540-77588-1},
  doi       = {10.1007\%2F978-3-540-77587-4_214},
  pages     = {2891 -- 2902},
  year      = {2010},
  language  = {en}
}
@book{Tippkoetter2010,
  author    = {Tippk{\"o}tter, Nils},
  title     = {Reaktionssysteme zur Aufarbeitung und Umsetzung nachwachsender Rohstoffe : Einsatz chromatographischer Verfahren sowie Membran- und Festbettreaktoren zur Verarbeitung von Molke, St{\"a}rke und Cellulose},
  publisher = {Logos-Verlag},
  address   = {Berlin},
  isbn      = {978-3-8325-2717-4},
  pages     = {III, 269 Seiten},
  year      = {2010},
  language  = {de}
}
@inproceedings{PothMonzonTippkoetteretal.2010,
  author    = {Poth, Sebastian and Monzon, Magaly and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Lignocellulosic biorefinery : process integration of hydrolysis and fermentation},
  series = {Proceedings / 11th European Workshop on Lignocellulosics and Pulp : August 16 - 19, 2010, Hamburg, Germany},
  booktitle = {Proceedings / 11th European Workshop on Lignocellulosics and Pulp : August 16 - 19, 2010, Hamburg, Germany},
  publisher = {vTi},
  address   = {Hamburg},
  pages     = {65 -- 68},
  year      = {2010},
  language  = {en}
}
@article{TippkoetterWollnyKampeisetal.2011,
  author    = {Tippk{\"o}tter, Nils and Wollny, S. and Kampeis, P. and Oster, J. and Schneider, H. and Ulber, R.},
  title     = {Magnetseparation von Proteinen : Separation von Zielmolek{\"u}len durch hochselektive Aptamere},
  series = {GIT Labor-Fachzeitschrift},
  volume    = {55},
  journal   = {GIT Labor-Fachzeitschrift},
  number    = {10},
  publisher = {Wiley},
  address   = {Weinheim},
  pages     = {666},
  year      = {2011},
  abstract  = {Durch die Kombination von Oligonukleotid-Liganden (Aptameren) hoher Bindungsaffinit{\"a}ten mit hochselektiv abtrennbaren magnetisierbaren Mikropartikeln wird eine einstufige Separation von Zielmolek{\"u}len aus mikrobiologischen Produktionsans{\"a}tzen m{\"o}glich. Die Aptamere werden hierf{\"u}r reversibel auf den Partikeloberfl{\"a}chen gebunden und f{\"u}r die spezifische Isolierung von Bioprodukten eingesetzt. Die Abtrennung der beladenen Partikel erfolgt durch einen neuen Rotor-Stator-Separator mit Hochgradient-Magnetfeld.},
  language  = {de}
}
@article{SiekerUlberDimitrovaetal.2009,
  author    = {Sieker, Tim and Ulber, Roland and Dimitrova, Darina and Bart, Hans-J{\"o}rg and Neuner, Andreas and Heinzle, Elmar and Tippk{\"o}tter, Nils},
  title     = {Silage : Fermentationsrohstoff f{\"u}r die chemische Industrie?},
  series = {labor\&more},
  journal   = {labor\&more},
  number    = {2},
  pages     = {44 -- 45},
  year      = {2009},
  abstract  = {In Anbetracht des zu erwartenden R{\"u}ckgangs der Verf{\"u}gbarkeit fossiler Rohstoffe m{\"u}ssen nicht nur f{\"u}r den Energiesektor, sondern auch f{\"u}r die Herstellung industrieller Produkte alternative Rohstoffe gefunden werden. Ein Beispiel f{\"u}r einen nicht in Nahrungsmittelkonkurrenz stehenden nachwachsenden Rohstoff ist gr{\"u}ne Biomasse wie Gras und Klee. Diese lassen sich in Deutschland auf großen Fl{\"a}chen anbauen und enthalten eine Vielzahl potenzieller Substrate f{\"u}r Fermentationen.},
  language  = {de}
}
@article{AlKaidyKuthanHeringetal.2016,
  author    = {Al-Kaidy, Huschyar and Kuthan, Kai and Hering, Thomas and Tippk{\"o}tter, Nils},
  title     = {Aqueous droplets used as enzymatic microreactors and their electromagnetic actuation},
  series = {Journal of Visualized Experiments},
  journal   = {Journal of Visualized Experiments},
  number    = {Issue 126},
  issn      = {1940-087X},
  doi       = {10.3791/54643},
  year      = {2016},
  abstract  = {For the successful implementation of microfluidic reaction systems, such as PCR and electrophoresis, the movement of small liquid volumes is essential. In conventional lab-on-a-chip-platforms, solvents and samples are passed through defined microfluidic channels with complex flow control installations. The droplet actuation platform presented here is a promising alternative. With it, it is possible to move a liquid drop (microreactor) on a planar surface of a reaction platform (lab-in-a-drop). The actuation of microreactors on the hydrophobic surface of the platform is based on the use of magnetic forces acting on the outer shell of the liquid drops which is made of a thin layer of superhydrophobic magnetite particles. The hydrophobic surface of the platform is needed to avoid any contact between the liquid core and the surface to allow a smooth movement of the microreactor. On the platform, one or more microreactors with volumes of 10 µL can be positioned and moved simultaneously. The platform itself consists of a 3 x 3 matrix of electrical double coils which accommodate either neodymium or iron cores. The magnetic field gradients are automatically controlled. By variation of the magnetic field gradients, the microreactors' magnetic hydrophobic shell can be manipulated automatically to move the microreactor or open the shell reversibly. Reactions of substrates and corresponding enzymes can be initiated by merging the microreactors or bringing them into contact with surface immobilized catalysts.},
  language  = {en}
}
@article{WulfhorstDuweMerseburgetal.2016,
  author    = {Wulfhorst, Helene and Duwe, Anna-Maria and Merseburg, Johannes and Tippk{\"o}tter, Nils},
  title     = {Compositional analysis of pretreated (beech) wood using differential scanning calorimetry and multivariate data analysis},
  series = {Tetrahedron},
  volume    = {72},
  journal   = {Tetrahedron},
  number    = {46},
  publisher = {Elsevier},
  address   = {Amsterdam},
  doi       = {10.1016/j.tet.2016.04.029},
  pages     = {7329 -- 7334},
  year      = {2016},
  abstract  = {The composition of plant biomass varies depending on the feedstock and pre-treatment conditions and influences its processing in biorefineries. In order to ensure optimal process conditions, the quantitative proportion of the main polymeric components of the pre-treated biomass has to be determined. Current standard procedures for biomass compositional analysis are complex, the measurements are afflicted with errors and therefore often not comparable. Hence, new powerful analytical methods are urgently required to characterize biomass. In this contribution, Differential Scanning Calorimetry (DSC) was applied in combination with multivariate data analysis (MVA) to detect the cellulose content of the plant biomass pretreated by Liquid Hot Water (LHW) and Organosolv processes under various conditions. Unlike conventional techniques, the developed analytic method enables the accurate quantification of monosaccharide content of the plant biomass without any previous sample preparation. It is easy to handle and avoids errors in sample preparation.},
  language  = {en}
}
@article{TippkoetterStueckmannKrolletal.2009,
  author    = {Tippk{\"o}tter, Nils and St{\"u}ckmann, Henning and Kroll, Stephen and Winkelmann, Gunda and Noack, Udo and Scheper, Thomas and Ulber, Roland},
  title     = {A semi-quantitative dipstick assay for microcystin},
  series = {Analytical and Bioanalytical Chemistry},
  volume    = {394},
  journal   = {Analytical and Bioanalytical Chemistry},
  number    = {3},
  publisher = {springer},
  address   = {Berlin},
  issn      = {1618-2650},
  doi       = {10.1007/s00216-009-2750-8},
  pages     = {863 -- 869},
  year      = {2009},
  abstract  = {An immunochromatographic lateral flow dipstick assay for the fast detection of microcystin-LR was developed. Colloid gold particles with diameters of 40 nm were used as red-colored antibody labels for the visual detection of the antigen. The new dipstick sensor is capable of detecting down to 5 µg·l-1 (ppb; total inversion of the color signal) or 1 ppb (observation of color grading) of microcystin-LR. The course of the labeling reaction was observed via spectrometric wave shifts caused by the change of particle size during the binding of antibodies. Different stabilizing reagents showed that especially bovine serum albumin (BSA) and casein increase the assays sensitivity and the conjugate stability. Performance of the dipsticks was quantified by pattern processing of capture zone CCD images. Storage stability of dipsticks and conjugate suspensions over 115 days under different conditions were monitored. The ready-to-use dipsticks were successfully tested with microcystin-LR-spiked samples of outdoor drinking- and salt water and applied to the tissue of microcystin-fed mussels.},
  language  = {en}
}
@article{UlberTippkoetter2009,
  author    = {Ulber, Roland and Tippk{\"o}tter, Nils},
  title     = {Nitratfreie Molke},
  series = {Rundschau f{\"u}r Fleischhygiene und Lebensmittel{\"u}berwachung},
  journal   = {Rundschau f{\"u}r Fleischhygiene und Lebensmittel{\"u}berwachung},
  number    = {4},
  pages     = {150 -- 152},
  year      = {2009},
  language  = {de}
}
@inproceedings{EngelThieringerTippkoetter2016,
  author    = {Engel, M. and Thieringer, J. and Tippk{\"o}tter, Nils},
  title     = {Microbial electrosynthesis for sustainable biobutanol production},
  series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  publisher = {DECHEMA},
  address   = {Frankfurt am Main},
  pages     = {77 -- 78},
  year      = {2016},
  language  = {en}
}
@inproceedings{HeringUlberTippkoetter2016,
  author    = {Hering, T. and Ulber, Roland and Tippk{\"o}tter, Nils},
  title     = {Development of a screening system for antimicrobial surfaces},
  series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  publisher = {DECHEMA},
  address   = {Frankfurt am Main},
  pages     = {129},
  year      = {2016},
  language  = {en}
}
@inproceedings{RothMoehringTippkoetter2016,
  author    = {Roth, J. and M{\"o}hring, S. and Tippk{\"o}tter, Nils},
  title     = {Characterization and evaluation of lignocellulosic biomass 130 hydrolysates for ABE fermentation},
  series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  publisher = {DECHEMA},
  address   = {Frankfurt am Main},
  pages     = {130},
  year      = {2016},
  language  = {en}
}
@inproceedings{MoehringWulfhorstRothetal.2016,
  author    = {M{\"o}hring, S. and Wulfhorst, H. and Roth, J. and Tippk{\"o}tter, Nils},
  title     = {Pretreatment strategies for lignocellulosic biomass},
  series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  publisher = {DECHEMA},
  address   = {Frankfurt am Main},
  pages     = {131},
  year      = {2016},
  language  = {en}
}
@inproceedings{CapitainHeringTippkoetteretal.2016,
  author    = {Capitain, C. and Hering, T. and Tippk{\"o}tter, Nils and Ulber, R.},
  title     = {Enzymatic polymerization of lignin model compounds and solubilized lignin in an aqueous ethanol extract},
  series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  publisher = {DECHEMA},
  address   = {Frankfurt am Main},
  pages     = {151 -- 152},
  year      = {2016},
  language  = {en}
}
@inproceedings{WulfhorstDuweMoehringetal.2016,
  author    = {Wulfhorst, H. and Duwe, A. and M{\"o}hring, S. and Jurca, O. and Tippk{\"o}tter, Nils},
  title     = {Analysis of pretreated biomass by differential scanning 132 calorimetry and multivariate data analysis},
  series = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  booktitle = {New frontiers of biotech-processes (Himmelfahrtstagung) : 02-04 May 2016, Rhein-Mosel-Halle, Koblenz/Germany},
  publisher = {DECHEMA},
  address   = {Frankfurt am Main},
  pages     = {132},
  year      = {2016},
  language  = {en}
}
@inproceedings{DeterdingTippkoetterUlber2006,
  author    = {Deterding, A. and Tippk{\"o}tter, Nils and Ulber, R.},
  title     = {Online-Essigs{\"a}ureanalytik in Fermentationsbr{\"u}hen mittels Fließdiffusionstechnik (FDT)},
  series = {Technische Systeme f{\"u}r Biotechnologie und Umwelt : 13. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 25.09. - 27.09.2006},
  booktitle = {Technische Systeme f{\"u}r Biotechnologie und Umwelt : 13. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 25.09. - 27.09.2006},
  editor    = {Beckmann, Dieter},
  address   = {Heiligenstadt},
  organization    = {Institut f{\"u}r Bioprozeß- und Analysenmeßtechnik},
  isbn      = {978-3-00-018621-9},
  pages     = {273 -- 280},
  year      = {2006},
  language  = {de}
}
@inproceedings{TippkoetterSchuenemannChristmannetal.2008,
  author    = {Tippk{\"o}tter, Nils and Sch{\"u}nemann, V. and Christmann, R. and Pasteur, A. and Schweizer, J. and Ulber, R.},
  title     = {Bioaffinity Layering magnetisierbarer Mikro- und Nanopartikel},
  series = {Technische Systeme f{\"u}r die Lebenswissenschaften : 14. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 22.09. - 24.09.2008},
  booktitle = {Technische Systeme f{\"u}r die Lebenswissenschaften : 14. Heiligenst{\"a}dter Kolloquium, Heilbad Heiligenstadt, 22.09. - 24.09.2008},
  editor    = {Beckmann, Dieter},
  address   = {Heiligenstadt},
  isbn      = {978-3-00-025695-0},
  pages     = {97 -- 98},
  year      = {2008},
  language  = {de}
}
@inproceedings{SiekerDuwePothetal.2012,
  author    = {Sieker, T. and Duwe, A. and Poth, S. and Tippk{\"o}tter, Nils and Ulber, R.},
  title     = {Herstellung von Itacons{\"a}ure aus Buchenholzhydrolysaten},
  series = {Kurzfassungsband / GVC-DECHEMA Vortrags- und Diskussionstagung Biopharmazeutische Produktion : 14. - 16. Mai 2012. Konzerthaus Freibung},
  booktitle = {Kurzfassungsband / GVC-DECHEMA Vortrags- und Diskussionstagung Biopharmazeutische Produktion : 14. - 16. Mai 2012. Konzerthaus Freibung},
  publisher = {DECHEMA},
  address   = {Frankfurt, M.},
  pages     = {57},
  year      = {2012},
  language  = {de}
}
@misc{AlKaidyTippkoetterUlber2014,
  author    = {Al-Kaidy, Huschyar and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {Vorrichtung und Verfahren zur Bestimmung des Kontaktwinkels eines fl{\"u}ssigen oder mit Fl{\"u}ssigkeit gef{\"u}llten K{\"o}rpers [Offenlegungsschrift]},
  publisher = {Deutsches Patent- und Markenamt},
  address   = {M{\"u}nchen},
  pages     = {13 Seiten},
  year      = {2014},
  abstract  = {Die vorliegende Erfindung betrifft eine Vorrichtung und ein Verfahren zur Bestimmung des Kontaktwinkels eines fl{\"u}ssigen oder mit Fl{\"u}ssigkeit gef{\"u}llten K{\"o}rpers. Dieser besteht aus einem Tr{\"a}ger (1) und einer damit verbundenen, in einem Winkelbereich von mehr als 0° bis maximal 90° neigbaren Ebene (8) mit einer darin ausgebildeten Abrollbahn (9) f{\"u}r den fl{\"u}ssigen oder mit Fl{\"u}ssigkeit gef{\"u}llten K{\"o}rper. An der Ebene (8) sind mehrere Sensoren (11, 12) zur Erfassung der Rolldauer des K{\"o}rpers entlang der Rollstrecke angeordnet. Erfindungsgem{\"a}ß ist vorgesehen, dass die Einstellung des Neigungswinkels der Ebene (8) {\"u}ber ein Winkelmessger{\"a}t (10) erfolgt, wodurch ein Abrollwinkel erfassbar ist, bei dem der K{\"o}rper in Bewegung ger{\"a}t. Aus der Rolldauer, der Rollstrecke und dem Abrollwinkel wird der Kontaktwinkel des K{\"o}rpers ermittelt.},
  language  = {de}
}
@misc{AlKaidyTippkoetterUlber2013,
  author    = {Al-Kaidy, Huschyar and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {A system and a method for the implementation of chemical, biological or physical reactions [Europ{\"a}ische Patentanmeldung]},
  publisher = {Europ{\"a}isches Patentamt},
  address   = {Den Hague},
  pages     = {16 Seiten},
  year      = {2013},
  abstract  = {The invention relates to a system for the implementation of chemical, biological or physical reactions, consisting of - one or more magnetic micro-reactors, each comprising a shell made of hydrophobic magnetic nanoparticles encapsulating an aqueous core, - a plane platform comprising a surface to receive the micro-reactors, - a source that generates a magnetic field above or underneath the platform for manipulating the one or more hydrophobic magnetic micro-reactors, or for moving them along the surface of the platform from one position to another position, characterized in that the aqueous core of the one or more magnetic micro-reactors contains a reaction solution or buffer, and wherein the magnetic field generated by the source correlates to a defined position on the surface of the platform.},
  language  = {en}
}
@misc{StadtmuellerTippkoetterUlber2013,
  author    = {Stadtm{\"u}ller, Ralf and Tippk{\"o}tter, Nils and Ulber, Roland},
  title     = {A method for production of single-stranded nucleic acids [Europ{\"a}ische Patentanmeldung]},
  publisher = {Europ{\"a}isches Patentamt},
  address   = {Den Hague},
  pages     = {14 Seiten},
  year      = {2013},
  language  = {en}
}
@article{TippkoetterRoikaewUlber2007,
  author    = {Tippk{\"o}tter, Nils and Roikaew, N. and Ulber, R.},
  title     = {Nitratentfernung aus Molkekonzentrat mit biotechnologischer Regeneration der Abw{\"a}sser},
  series = {Deutsche Milchwirtschaft},
  volume    = {58},
  journal   = {Deutsche Milchwirtschaft},
  number    = {15},
  issn      = {0012-0480},
  pages     = {540 -- 542},
  year      = {2007},
  language  = {de}
}
@article{TippkoetterRoikaewUlber2008,
  author    = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, R.},
  title     = {Nitrate removal from whey concentrate with biotechnological regeneration of the waste water},
  series = {European dairy magazine : EDM},
  journal   = {European dairy magazine : EDM},
  number    = {1},
  isbn      = {0936-6318},
  pages     = {30 -- 32},
  year      = {2008},
  language  = {en}
}
@article{UlberTippkoetterBuchholzetal.2008,
  author    = {Ulber, R. and Tippk{\"o}tter, Nils and Buchholz, H. and Demmer, W. and Scheper, T.},
  title     = {Innovative Verfahren in der Molkeaufarbeitung zur Gewinnung neuer Produkte},
  series = {Deutsche Milchwirtschaft},
  volume    = {59},
  journal   = {Deutsche Milchwirtschaft},
  number    = {19},
  issn      = {0012-0480},
  pages     = {704 -- 706},
  year      = {2008},
  language  = {de}
}
@inproceedings{AlKaidyUlberTippkoetter2014,
  author    = {Al-Kaidy, H. and Ulber, R. and Tippk{\"o}tter, Nils},
  title     = {A platform technology for the automated reaction control in magnetizable micro-fluidic droplets},
  series = {Biomaterials - made in bioreactors : book of abstracts, May 26 - 28, 2014, Radisson Blu Park Hotel and Conference Dentre, Radebeul, Germany},
  booktitle = {Biomaterials - made in bioreactors : book of abstracts, May 26 - 28, 2014, Radisson Blu Park Hotel and Conference Dentre, Radebeul, Germany},
  publisher = {DECHEMA},
  address   = {Frankfurt am Main},
  pages     = {21 -- 22},
  year      = {2014},
  language  = {en}
}
@inproceedings{TippkoetterRoikaewUlber2008,
  author    = {Tippk{\"o}tter, Nils and Roikaew, W. and Ulber, R.},
  title     = {An automated pilot plant for the bioengineering processing of concentrated whey},
  series = {European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme},
  booktitle = {European BioPerspectives : in cooperation with BIOTECHNICA 2008 : 7 - 9 October 2008 Hannover, Germany ; book of abstracts ; abstracts, poster programme},
  publisher = {Dechema},
  address   = {Frankfurt am Main},
  pages     = {98},
  year      = {2008},
  language  = {en}
}
@inproceedings{TippkoetterMoehringMaureretal.2013,
  author    = {Tippk{\"o}tter, Nils and M{\"o}hring, S. and Maurer, S. and Roth, J.},
  title     = {Dezentrale Vorbehandlung und Verarbeitung pflanzlicher Reststoffe f{\"u}r Bioraffinerien},
  series = {Kurzfassungen der Vortr{\"a}ge nach Sessions : Fr{\"u}hjahrstagung der Biotechnologen 2013, 4. - 5. M{\"a}rz 2013, Dechema-Haus, Frankfurt am Main},
  booktitle = {Kurzfassungen der Vortr{\"a}ge nach Sessions : Fr{\"u}hjahrstagung der Biotechnologen 2013, 4. - 5. M{\"a}rz 2013, Dechema-Haus, Frankfurt am Main},
  address   = {Frankfurt am Main},
  pages     = {5},
  year      = {2013},
  language  = {de}
}
@inproceedings{Tippkoetter2013,
  author    = {Tippk{\"o}tter, Nils},
  title     = {Biotechnologische Gewinnung von Wertstoffen aus Molke : BiobasedWorld - Innovation in food},
  series = {Biotechnica 2013 : European biotechnology science \& industry news},
  volume    = {12},
  booktitle = {Biotechnica 2013 : European biotechnology science \& industry news},
  number    = {9, special},
  pages     = {33 -- 50},
  year      = {2013},
  language  = {de}
}
@inproceedings{TippkoetterStueckmannWinkelmannetal.2007,
  author    = {Tippk{\"o}tter, Nils and St{\"u}ckmann, H. and Winkelmann, G. and Noack, U. and Beutel, S. and Scheper, T. and Ulber, R.},
  title     = {Optimisation of antibody-labelling of gold colloids for their application in an immunchromatographic assay for microcystin-LR},
  series = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme},
  booktitle = {European BioPerspectives : celebrating the 25th DECHEMA annual convention of biotechnologists ; 30 May - 1 June 2007, Cologne, Germany ; book of abstracts ; abstracts, poster programme},
  publisher = {Dechema},
  address   = {Frankfurt am Main},
  pages     = {126},
  year      = {2007},
  language  = {en}
}
@incollection{WagemannTippkoetter2019,
  author    = {Wagemann, Kurt and Tippk{\"o}tter, Nils},
  title     = {Biorefineries: a short introduction},
  series = {Biorefineries},
  booktitle = {Biorefineries},
  publisher = {Springer},
  address   = {Cham},
  isbn      = {978-3-319-97117-9},
  doi       = {10.1007/10_2017_4},
  pages     = {1 -- 11},
  year      = {2019},
  abstract  = {The terms bioeconomy and biorefineries are used for a variety of processes and developments. This short introduction is intended to provide a delimitation and clarification of the terminology as well as a classification of current biorefinery concepts. The basic process diagrams of the most important biorefinery types are shown.},
  language  = {en}
}
@incollection{UlberMufflerTippkoetteretal.2011,
  author    = {Ulber, Roland and Muffler, Kai and Tippk{\"o}tter, Nils and Hirth, Thomas and Sell, Dieter},
  title     = {Introduction to Renewable Resources in the Chemical Industry},
  series = {Renewable raw materials : new feedstocks for the chemical industry},
  booktitle = {Renewable raw materials : new feedstocks for the chemical industry},
  editor    = {Ulber, Roland and Sell, Dieter and Hirth, Thomas},
  edition   = {1. Auflage},
  publisher = {Wiley-VCH-Verlag},
  address   = {Weinheim},
  isbn      = {978-3-527-32548-1},
  pages     = {1 -- 6},
  year      = {2011},
  language  = {de}
}
@article{TeumerCapitainRossJonesetal.2018,
  author    = {Teumer, T. and Capitain, C. and Ross-Jones, J. and Tippk{\"o}tter, Nils and R{\"a}dle, M. and Methner, F.-J.},
  title     = {In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor},
  series = {BrewingScience},
  volume    = {71},
  journal   = {BrewingScience},
  number    = {5/6},
  publisher = {Fachverlag Hans Carl},
  address   = {N{\"u}rnberg},
  issn      = {1613-2041},
  pages     = {49 -- 55},
  year      = {2018},
  abstract  = {In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented.},
  language  = {en}
}
@incollection{TippkoetterMoehringRothetal.2019,
  author    = {Tippk{\"o}tter, Nils and M{\"o}hring, Sophie and Roth, Jasmine and Wulfhorst, Helene},
  title     = {Logistics of lignocellulosic feedstocks: preprocessing as a preferable option},
  series = {Biorefineries},
  booktitle = {Biorefineries},
  publisher = {Springer},
  address   = {Cham},
  isbn      = {978-3-319-97117-9},
  doi       = {10.1007/10_2017_58},
  pages     = {43 -- 68},
  year      = {2019},
  abstract  = {In comparison to crude oil, biorefinery raw materials are challenging in concerns of transport and storage. The plant raw materials are more voluminous, so that shredding and compacting usually are necessary before transport. These mechanical processes can have a negative influence on the subsequent biotechnological processing and shelf life of the raw materials. Various approaches and their effects on renewable raw materials are shown. In addition, aspects of decentralized pretreatment steps are discussed. Another important aspect of pretreatment is the varying composition of the raw materials depending on the growth conditions. This problem can be solved with advanced on-site spectrometric analysis of the material.},
  language  = {en}
}
@incollection{DuweTippkoetterUlber2018,
  author    = {Duwe, A. and Tippk{\"o}tter, Nils and Ulber, R.},
  title     = {Lignocellulose-Biorefinery: Ethanol-Focused},
  series = {Biorefineries},
  booktitle = {Biorefineries},
  publisher = {Springer},
  address   = {Cham},
  doi       = {10.1007/10_2016_72},
  pages     = {177 -- 215},
  year      = {2018},
  abstract  = {The development prospects of the world markets for petroleum and other liquid fuels are diverse and partly contradictory. However, comprehensive changes for the energy supply of the future are essential. Notwithstanding the fact that there are still very large deposits of energy resources from a geological point of view, the finite nature of conventional oil reserves is indisputable. To reduce our dependence on oil, the EU, the USA, and other major economic zones rely on energy diversification. For this purpose, alternative materials and technologies are being sought, and is most obvious in the transport sector. The objective is to progressively replace fossil fuels with renewable and more sustainable fuels. In this respect, biofuels have a pre-eminent position in terms of their capability of blending with fossil fuels and being usable in existing cars without substantial modification. Ethanol can be considered as the primary renewable liquid fuel. In this chapter enzymes, micro-organisms, and processes for ethanol production based on renewable resources are described.},
  language  = {en}
}
@techreport{Tippkoetter2018,
  author    = {Tippk{\"o}tter, Nils},
  title     = {Lokale Vorbehandlung nachwachsender Rohstoffe f{\"u}r Bioraffinerien (BioSats) : Schlussbericht zum Vorhaben : Laufzeit: 01.03.2012 bis 30.04.2017},
  organization    = {Technische Universit{\"a}t Kaiserslautern},
  doi       = {10.2314/GBV:1024204243},
  pages     = {191 Seiten},
  year      = {2018},
  language  = {de}
}
@article{EngelHoltmannUlberetal.2018,
  author    = {Engel, Mareike and Holtmann, Dirk and Ulber, Roland and Tippk{\"o}tter, Nils},
  title     = {Increased Biobutanol Production by Mediator-Less Electro-Fermentation},
  series = {Biotechnology Journal},
  journal   = {Biotechnology Journal},
  number    = {Volume 14, Issue 4},
  publisher = {Wiley-VCH},
  issn      = {1860-7314},
  doi       = {10.1002/biot.201800514},
  pages     = {Artikel 1800514},
  year      = {2018},
  abstract  = {A future bio-economy should not only be based on renewable raw materials but also in the raise of carbon yields of existing production routes. Microbial electrochemical technologies are gaining increased attention for this purpose. In this study, the electro-fermentative production of biobutanol with C. acetobutylicum without the use of exogenous mediators is investigated regarding the medium composition and the reactor design. It is shown that the use of an optimized synthetic culture medium allows higher product concentrations, increased biofilm formation, and higher conductivities compared to a synthetic medium supplemented with yeast extract. Moreover, the optimization of the reactor system results in a doubling of the maximum product concentrations for fermentation products. When a working electrode is polarized at -600 mV vs. Ag/AgCl, a shift from butyrate to acetone and butanol production is induced. This leads to an increased final solvent yield of Yᴀᴃᴇ = 0.202 gg⁻¹ (control 0.103 gg⁻¹), which is also reflected in a higher carbon efficiency of 37.6\% compared to 23.3\% (control) as well as a fourfold decrease in simplified E-factor to 0.43. The results are promising for further development of biobutanol production in bioelectrochemical systems in order to fulfil the principles of Green Chemistry.},
  language  = {en}
}
@book{WagemannTippkoetter2019,
  author    = {Wagemann, Kurt and Tippk{\"o}tter, Nils},
  title     = {Biorefineries / Kurt Wagemann, Nils Tippk{\"o}tter (editors)},
  series = {Advances in biochemical engineering/biotechnology book series (ABE)},
  journal   = {Advances in biochemical engineering/biotechnology book series (ABE)},
  publisher = {Springer},
  address   = {Cham (Switzerland)},
  isbn      = {978-3-319-97117-9},
  doi       = {10.1007/978-3-319-97119-3},
  pages     = {VI, 549 Seiten},
  year      = {2019},
  language  = {en}
}