@article{TuegBaumann1995,
  author    = {T{\"u}g, Helmut and Baumann, Marcus},
  title     = {Reply to the comments by R.L. McKenzie and P.V. Johnston on our paper "Problems of UV-B radiation measurements in biological research: Critical Remarks on current techniques and suggestions for improvements"},
  series = {Geophysical research letters},
  volume    = {Vol. 22},
  journal   = {Geophysical research letters},
  number    = {Iss. 9},
  issn      = {1944-8007 (E-Journal); 0094-8276 (Print)},
  pages     = {1159 -- 1160},
  year      = {1995},
  language  = {en}
}
@inproceedings{BrandiniBaumann1997,
  author    = {Brandini, Frederico P. and Baumann, Marcus},
  title     = {The potential role of melted 'brown ice' as sources of chelators and ammonia to the surface waters of the Weddell Sea, Antarctica},
  series = {Proceedings of the NIPR Symposium on Polar Biology : 10},
  booktitle = {Proceedings of the NIPR Symposium on Polar Biology : 10},
  address   = {Tokyo},
  organization    = {National Institute of Polar Research},
  issn      = {0914-563X},
  pages     = {1 -- 13},
  year      = {1997},
  language  = {en}
}
@misc{BesslerMaurerMerkeletal.2009,
  author    = {Bessler, Cornelius and Maurer, Karl-Heinz and Merkel, Marion and Siegert, Petra and Wieland, Susanne},
  title     = {Subtilisin from Bacillus Pumilus and detergent and cleaning agents containing said novel subtilisin [US Patentanmeldung / Internationale Patentanmeldung]},
  publisher = {USPTO; WIPO},
  address   = {Washington; Genf},
  pages     = {1 -- 39},
  year      = {2009},
  language  = {en}
}
@article{MartinezJakobTuetal.2013,
  author    = {Martinez, Ronny and Jakob, Felix and Tu, Ran and Siegert, Petra and Maurer, Karl-Heinz and Schwaneberg, Ulrich},
  title     = {Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution},
  series = {Biotechnology and bioengineering},
  volume    = {Vol. 110},
  journal   = {Biotechnology and bioengineering},
  number    = {Iss. 3},
  publisher = {Wiley},
  address   = {Weinheim},
  issn      = {1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print)},
  pages     = {711 -- 720},
  year      = {2013},
  language  = {en}
}
@article{JakobMartinezMandaweetal.2013,
  author    = {Jakob, Felix and Martinez, Ronny and Mandawe, John and Hellmuth, Hendrik and Siegert, Petra and Maurer, Karl-Heinz and Schwaneberg, Ulrich},
  title     = {Surface charge engineering of a Bacillus gibsonii subtilisin protease},
  series = {Applied microbiology and biotechnology},
  volume    = {Vol. 97},
  journal   = {Applied microbiology and biotechnology},
  number    = {Iss. 15},
  publisher = {Springer},
  address   = {Berlin},
  issn      = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)},
  pages     = {6793 -- 6802},
  year      = {2013},
  language  = {en}
}
@article{NiehausGaborWielandetal.2011,
  author    = {Niehaus, F. and Gabor, E. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Eck, J.},
  title     = {Enzymes for the laundry industries: tapping the vast metagenomic pool of alkaline proteases},
  series = {Microbial biotechnology},
  volume    = {Vol. 4},
  journal   = {Microbial biotechnology},
  number    = {Iss. 6},
  publisher = {Springer},
  address   = {Berlin},
  issn      = {1432-0614 (E-Journal); 0171-1741 (Print); 0175-7598 (Print); 0340-2118 (Print)},
  pages     = {767 -- 776},
  year      = {2011},
  language  = {en}
}
@article{RibitschHeumannKarletal.2012,
  author    = {Ribitsch, D. and Heumann, S. and Karl, W. and Gerlach, J. and Leber, R. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Siegert, Petra and Lange, J. and Maurer, Karl-Heinz and Berg, G. and Guebitz, G. M. and Schwab, H.},
  title     = {Extracellular serine proteases from Stenotrophomonas maltophilia: Screening, isolation and heterologous expression in E. coli},
  series = {Journal of biotechnology},
  volume    = {157},
  journal   = {Journal of biotechnology},
  number    = {1},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1873-4863 (E-Journal); 0168-1656 (Print)},
  doi       = {10.1016/j.jbiotec.2011.09.025},
  pages     = {140 -- 147},
  year      = {2012},
  abstract  = {A large strain collection comprising antagonistic bacteria was screened for novel detergent proteases. Several strains displayed protease activity on agar plates containing skim milk but were inactive in liquid media. Encapsulation of cells in alginate beads induced protease production. Stenotrophomonas maltophilia emerged as best performer under washing conditions. For identification of wash-active proteases, four extracellular serine proteases called StmPr1, StmPr2, StmPr3 and StmPr4 were cloned. StmPr2 and StmPr4 were sufficiently overexpressed in E. coli. Expression of StmPr1 and StmPr3 resulted in unprocessed, insoluble protein. Truncation of most of the C-terminal domain which has been identified by enzyme modeling succeeded in expression of soluble, active StmPr1 but failed in case of StmPr3. From laundry application tests StmPr2 turned out to be a highly wash-active protease at 45 °C. Specific activity of StmPr2 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 17 ± 2 U/mg. In addition we determined the kinetic parameters and cleavage preferences of protease StmPr2.},
  language  = {en}
}
@article{RibitschKarlBirnerGruenbergeretal.2010,
  author    = {Ribitsch, D. and Karl, W. and Birner-Gruenberger, R. and Gruber, K. and Eiteljoerg, I. and Remler, P. and Wieland, S. and Siegert, Petra and Maurer, Karl-Heinz and Schwab, H.},
  title     = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli},
  series = {Journal of biotechnology},
  volume    = {150},
  journal   = {Journal of biotechnology},
  number    = {3},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1873-4863 (E-Journal); 0168-1656 (Print)},
  doi       = {10.1016/j.jbiotec.2010.09.947},
  pages     = {408 -- 416},
  year      = {2010},
  abstract  = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.},
  language  = {en}
}
@incollection{WendorffEggertPohletal.2007,
  author    = {Wendorff, Marion and Eggert, Thorsten and Pohl, Martina and Dresen, Carola and M{\"u}ller, Michael and Jaeger, Karl-Erich and Sprenger, Georg A. and Sch{\"u}rmann, Melanie and Sch{\"u}rmann, Martin and Johnen, Sandra and Sprenger, Gerda and Sahm, Hermann and Inoue, Tomoyuki and Sch{\"o}rken, Ulrich and Breittaupt, Holger and Fr{\"o}lich, Bettina and Heim, Petra and Iding, Hans and Juchem, Bettina and Siegert, Petra and Kula, Maria-Regina and Weckbecker, Andrea and Hummel, Werner and Fessner, Wolf-Dieter and Elling, Lothar and Wolberg, Michael and Bode, Silke and Feldmann, Ralf and Geilenkirchen, Petra and Schubert, Thomas and Walter, Lydia and D{\"u}nnwald, Thomas and Demir, Ayhan S. and Kolter-Jung, Doris and Nitsche, Adam and D{\"u}nkelmann, Pascal and Cosp, Annabel and Lingen, Bettina},
  title     = {Catalytic asymmetric synthesis : section 2.2},
  series = {Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ...},
  booktitle = {Asymmetric synthesis with chemical and biological methods / ed. by Dieter Enders ...},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  isbn      = {978-3-527-31473-7},
  pages     = {298 -- 413},
  year      = {2007},
  language  = {en}
}
@article{SiegertMcLeishBaumannetal.2005,
  author    = {Siegert, Petra and McLeish, Michael J. and Baumann, Martin and Iding, Hans and Kneen, Malea M. and Kenyon, George L. and Pohl, Martina},
  title     = {Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida},
  series = {Protein engineering, design, and selection : peds},
  volume    = {Vol. 18},
  journal   = {Protein engineering, design, and selection : peds},
  number    = {Iss. 7},
  issn      = {1460-213X (E-Journal); 1741-0134 (E-Journal); 0269-2139 (Print); 1741-0126 (Print)},
  pages     = {345 -- 357},
  year      = {2005},
  language  = {en}
}
@incollection{SiegertPohlKneenetal.2004,
  author    = {Siegert, Petra and Pohl, Martina and Kneen, Malea M. and Pogozheva, Irina D. and Kenyon, George L. and McLeish, Michael J.},
  title     = {Exploring the substrate specificity of benzoylformate decarboxylase, pyruvate decarboxylase, and benzaldehyde lyase},
  series = {Thiamine : catalytic mechanisms in normal and disease states / ed. by Frank Jordan ...},
  booktitle = {Thiamine : catalytic mechanisms in normal and disease states / ed. by Frank Jordan ...},
  publisher = {Dekker},
  address   = {New York, NY},
  isbn      = {0-8247-4062-9},
  pages     = {275 -- 290},
  year      = {2004},
  language  = {en}
}
@article{DuenkelmannKolterJungNitscheetal.2002,
  author    = {D{\"u}nkelmann, Pascal and Kolter-Jung, Doris and Nitsche, Adam and Demir, Ayhan S. and Siegert, Petra and Lingen, Bettina and Baumann, Martin and Pohl, Martina and M{\"u}ller, Michael},
  title     = {Development of a donor-acceptor concept for enzymatic cross-coupling reactions of adehydes : the first asymmetric cross-benzoin condensation},
  series = {Journal of the American Chemical Society},
  volume    = {Vol. 124},
  journal   = {Journal of the American Chemical Society},
  issn      = {1520-5126 (E-Journal); 0002-7863 (Print)},
  pages     = {12084 -- 12085},
  year      = {2002},
  language  = {en}
}
@article{DuennwaldDemirSiegertetal.2001,
  author    = {D{\"u}nnwald, Thomas and Demir, Ayhan S. and Siegert, Petra and Pohl, Martina and M{\"u}ller, Michael},
  title     = {ChemInform Abstract: Enantioselective synthesis of (S)-2-Hydroxypropanone derivatives by Benzoylformate Decarboxylase Catalyzed C—C Bond Formation},
  series = {Cheminform},
  volume    = {Vol. 32},
  journal   = {Cheminform},
  number    = {Iss. 4},
  issn      = {1522-2667 (E-Journal); 0931-7597 (Print)},
  pages     = {Publ. online},
  year      = {2001},
  language  = {en}
}
@article{DuennwaldDemirSiegertetal.2000,
  author    = {D{\"u}nnwald, Thomas and Demir, Ayhan S. and Siegert, Petra and Pohl, Martina and M{\"u}ller, Michael},
  title     = {Enantioselective Synthesis of (S)-2-Hydroxypropanone Derivatives by Benzoylformate Decarboxylase Catalyzed C-C Bond Formation},
  series = {European journal of organic chemistry},
  volume    = {Vol. 2000},
  journal   = {European journal of organic chemistry},
  number    = {Iss. 11},
  issn      = {0365-5490 (E-Journal); 1099-0690 (E-Journal); 0075-4617 (Print); 0170-2041 (Print); 0947-3440 (Print); 1434-193X (Print); 1434-243X (Print)},
  pages     = {2161 -- 2170},
  year      = {2000},
  language  = {en}
}
@inproceedings{SiegertIdingBaumannetal.2000,
  author    = {Siegert, Petra and Iding, Hans and Baumann, Martin and McLeish, Michael J. and Kenyon, George L. and Pohl, Martina},
  title     = {Broadening of the substrate spectra of two ThDP-dependent decarboxylases using site-directed-mutagenesis},
  series = {Proceedings of the 4th International Congress on Biochemical Engineering : 17 and 18 February 2000, Stuttgart},
  booktitle = {Proceedings of the 4th International Congress on Biochemical Engineering : 17 and 18 February 2000, Stuttgart},
  organization    = {International Congress on Biochemical Engineering <4, 2000, Stuttgart>},
  isbn      = {3-8167-5570-4},
  pages     = {38 -- 42},
  year      = {2000},
  language  = {en}
}
@article{IdingDuennwaldGreineretal.2000,
  author    = {Iding, Hans and D{\"u}nnwald, Thomas and Greiner, Lasse and Liese, Andreas and M{\"u}ller, Michael and Siegert, Petra and Gr{\"o}tzinger, Joachim and Demir, Ayhan S. and Pohl, Martina},
  title     = {Benzoylformate Decarboxylase from Pseudomonas putida as Stable Catalyst for the Synthesis of Chiral 2-Hydroxy Ketones},
  series = {Chemistry - a European journal},
  volume    = {Vol. 6},
  journal   = {Chemistry - a European journal},
  number    = {Iss. 8},
  issn      = {1521-3765 (E-Journal); 0947-6539 (Print)},
  pages     = {1483 -- 1495},
  year      = {2000},
  language  = {en}
}
@article{IdingSiegertMeschetal.1998,
  author    = {Iding, Hans and Siegert, Petra and Mesch, K. and Pohl, Martina},
  title     = {Application of α-keto acid decarboxylases in biotransformations},
  series = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology},
  volume    = {Vol. 1385},
  journal   = {Biochimica et biophysica acta (BBA) - Protein structure and molecular enzymology},
  number    = {Iss. 2},
  issn      = {1879-2588 (E-Journal); 0167-4838 (Print)},
  pages     = {307 -- 322},
  year      = {1998},
  language  = {en}
}
@article{PohlSiegertMeschetal.1998,
  author    = {Pohl, Martina and Siegert, Petra and Mesch, K. and Bruhn, H. and Gr{\"o}tzinger, Joachim},
  title     = {Active site mutants of pyruvate decarboxylase from Zymomonas mobilis : a site-directed mutagenesis study of L112, I472, I476, E473 and N482},
  series = {European journal of biochemistry},
  volume    = {Vol. 257},
  journal   = {European journal of biochemistry},
  number    = {Iss. 3},
  issn      = {1432-1033 (E-Journal); 1742-4658 (E-Journal); 0014-2956 (Print); 1742-464X (Print)},
  pages     = {538 -- 546},
  year      = {1998},
  language  = {en}
}
@article{RatkeMilowLisinskietal.2014,
  author    = {Ratke, Lorenz and Milow, Barbara and Lisinski, Susanne and Hoepfner, Sandra},
  title     = {On an effect of fine ceramic particles on the structure of aerogels},
  series = {Microgravity science and technology},
  volume    = {26},
  journal   = {Microgravity science and technology},
  publisher = {Springer Nature},
  address   = {Heidelberg},
  issn      = {0938-0108 ; 1875-0494},
  doi       = {10.1007/s12217-014-9380-2},
  pages     = {103 -- 110},
  year      = {2014},
  language  = {en}
}
@article{HandtkeVollandMethlingetal.2014,
  author    = {Handtke, Stefan and Volland, Sonja and Methling, Karen and Albrecht, Dirk and Becher, D{\"o}rte and Nehls, Jenny and Bongaerts, Johannes and Maurer, Karl-Heinz and Lalk, Michael and Liesegang, Heiko and Voigt, Birgit and Daniel, Rolf and Hecker, Michael},
  title     = {Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach},
  series = {Journal of Biotechnology},
  journal   = {Journal of Biotechnology},
  number    = {192(A)},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1873-4863 (E-Journal); 0168-1656 (Print)},
  doi       = {10.1016/j.jbiotec.2014.08.028},
  pages     = {204 -- 214},
  year      = {2014},
  abstract  = {Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43\% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC-MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.},
  language  = {en}
}
@article{NachtrodtTietschMostaccietal.2014,
  author    = {Nachtrodt, Frederik and Tietsch, Wolfgang and Mostacci, Domiziano and Scherer, Ulrich W.},
  title     = {Set-up and first operation of a plasma oven for treatment of low level radioactive wastes},
  series = {Nuclear technology and radiation protection},
  volume    = {29},
  journal   = {Nuclear technology and radiation protection},
  number    = {Suppl.},
  publisher = {VINČA Institute of Nuclear Sciences},
  address   = {Belgrad},
  issn      = {1451-3994},
  doi       = {10.2298/NTRP140SS47N},
  pages     = {47 -- 51},
  year      = {2014},
  language  = {en}
}
@incollection{FrotscherGossmannRaatschenetal.2015,
  author    = {Frotscher, Ralf and Goßmann, Matthias and Raatschen, Hans-J{\"u}rgen and Temiz Artmann, Ayseg{\"u}l and Staat, Manfred},
  title     = {Simulation of cardiac cell-seeded membranes using the edge-based smoothed FEM},
  series = {Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45)},
  booktitle = {Shell and membrane theories in mechanics and biology. (Advanced structured materials ; 45)},
  publisher = {Springer},
  address   = {Heidelberg},
  isbn      = {978-3-319-02534-6 ; 978-3-319-02535-3},
  pages     = {187 -- 212},
  year      = {2015},
  abstract  = {We present an electromechanically coupled Finite Element model for cardiac tissue. It bases on the mechanical model for cardiac tissue of Hunter et al. that we couple to the McAllister-Noble-Tsien electrophysiological model of purkinje fibre cells. The corresponding system of ordinary differential equations is implemented on the level of the constitutive equations in a geometrically and physically nonlinear version of the so-called edge-based smoothed FEM for plates. Mechanical material parameters are determined from our own pressure-deflection experimental setup. The main purpose of the model is to further examine the experimental results not only on mechanical but also on electrophysiological level down to ion channel gates. Moreover, we present first drug treatment simulations and validate the model with respect to the experiments.},
  language  = {en}
}
@article{RaueWambachGloeggleretal.2014,
  author    = {Raue, Markus and Wambach, M. and Gl{\"o}ggler, S. and Grefen, Dana and Kaufmann, R. and Abetz, C. and Georgopanos, P. and Handge, U. A. and Mang, Thomas and Bl{\"u}mich, B. and Abetz, V.},
  title     = {Investigation of historical hard rubber ornaments of Charles Goodyear},
  series = {Macromolecular chemistry and physics},
  volume    = {Vol. 215},
  journal   = {Macromolecular chemistry and physics},
  number    = {No. 3},
  publisher = {Wiley-VCH},
  address   = {Weinheim},
  issn      = {1022-1352},
  pages     = {245 -- 254},
  year      = {2014},
  language  = {en}
}
@article{SeifarthGossmannGrosseetal.2015,
  author    = {Seifarth, Volker and Goßmann, Matthias and Grosse, J. O. and Becker, C. and Heschel, I. and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l},
  title     = {Development of a Bioreactor to Culture Tissue Engineered Ureters Based on the Application of Tubular OPTIMAIX 3D Scaffolds},
  series = {Urologia Internationalis},
  volume    = {2015},
  journal   = {Urologia Internationalis},
  number    = {95},
  publisher = {Karger},
  address   = {Basel},
  issn      = {0042-1138},
  doi       = {10.1159/000368419},
  pages     = {106 -- 113},
  year      = {2015},
  language  = {en}
}
@article{PilasIkenSelmeretal.2015,
  author    = {Pilas, Johanna and Iken, Heiko and Selmer, Thorsten and Keusgen, Michael and Sch{\"o}ning, Michael Josef},
  title     = {Development of a multi-parameter sensor chip for the simultaneous detection of organic compounds in biogas processes},
  series = {Physica status solidi (a)},
  volume    = {212},
  journal   = {Physica status solidi (a)},
  number    = {6},
  publisher = {Wiley},
  address   = {Weinheim},
  issn      = {1862-6319},
  doi       = {10.1002/pssa.201431894},
  pages     = {1306 -- 1312},
  year      = {2015},
  abstract  = {An enzyme-based multi-parameter biosensor is developed for monitoring the concentration of formate, d-lactate, and l-lactate in biological samples. The sensor is based on the specific dehydrogenation by an oxidized β-nicotinamide adenine dinucleotide (NAD+)-dependent dehydrogenase (formate dehydrogenase, d-lactic dehydrogenase, and l-lactic dehydrogenase, respectively) in combination with a diaphorase from Clostridium kluyveri (EC 1.8.1.4). The enzymes are immobilized on a platinum working electrode by cross-linking with glutaraldehyde (GA). The principle of the determination scheme in case of l-lactate is as follows: l-lactic dehydrogenase (l-LDH) converts l-lactate into pyruvate by reaction with NAD+. In the presence of hexacyanoferrate(III), the resulting reduced β-nicotinamide adenine dinucleotide (NADH) is then regenerated enzymatically by diaphorase. The electrochemical detection is based on the current generated by oxidation of hexacyanoferrate(II) at an applied potential of +0.3 V vs. an Ag/AgCl reference electrode. The biosensor will be electrochemically characterized in terms of linear working range and sensitivity. Additionally, the successful practical application of the sensor is demonstrated in an extract from maize silage.},
  language  = {en}
}
@article{BreuerRaueKirschbaumetal.2015,
  author    = {Breuer, Lars and Raue, Markus and Kirschbaum, M. and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, R. and Wagner, Torsten},
  title     = {Light-controllable polymeric material based on temperature-sensitive hydrogels with incorporated graphene oxide},
  series = {Physica status solidi (a)},
  volume    = {212},
  journal   = {Physica status solidi (a)},
  number    = {6},
  publisher = {Wiley},
  address   = {Weinheim},
  issn      = {1862-6319},
  doi       = {10.1002/pssa.201431944},
  pages     = {1368 -- 1374},
  year      = {2015},
  abstract  = {Poly(N-isopropylacrylamide) (PNIPAAm) hydrogel films with incorporated graphene oxide (GO) were developed and tested as light-stimulated actuators. GO dispersions were synthesized via Hummers method and characterized toward their optical properties and photothermal energy conversion. The hydrogels were prepared by means of photopolymerization. In addition, the influence of GO within the hydrogel network on the lower critical solution temperature (LCST) was investigated by differential scanning calorimetry (DSC). The optical absorbance and the response to illumination were determined as a function of GO concentration for thin hydrogel films. A proof of principle for the stimulation with light was performed.},
  language  = {en}
}
@article{RaabKappelKraemeretal.2011,
  author    = {Raab, Monika and Kappel, Sven and Kr{\"a}mer, Andrea and Sanhaji, Mourad and Matthess, Yves and Kurunci-Csacsko, Elisabeth and Calzada-Wack, Julia and Rathkolb, Birgit and Rosman, Jan and Adler, Thure and Busch, Dirk H. and Esposito, Irene and Fuchs, Helmut and Gailus-Durner, Val{\´e}rie and Klingenspor, Martin and Wolf, Eckhard and S{\"a}nger, Nicole and Prinz, Florian and Hrabe de Angelis, Martin and Seibler, Jost and Yuan, Juping and Bergmann, Martin and Knecht, Rainald and Kreft, Bertolt and Strebhardt, Klaus},
  title     = {Toxicity modelling of Plk1-targeted therapies in genetically engineered mice and cultured primary mammalian cells},
  series = {Nature Communications},
  volume    = {2},
  journal   = {Nature Communications},
  number    = {395},
  publisher = {Nature},
  address   = {London},
  issn      = {2041-1723},
  doi       = {10.1038/ncomms1395},
  pages     = {1 -- 11},
  year      = {2011},
  language  = {en}
}
@article{HasegawaKapelyukhTaharaetal.2011,
  author    = {Hasegawa, Maki and Kapelyukh, Yury and Tahara, Harunobu and Seibler, Jost and Rode, Anja and Krueger, Sylvia and Lee, Dongtao N. and Wolf, C. Roland and Scheer, Nico},
  title     = {Quantitative prediction of human pregnane X receptor and cytochrome P450 3A4 mediated drug-drug interaction in a novel multiple humanized mouse line},
  series = {Molecular Pharmacology},
  volume    = {80},
  journal   = {Molecular Pharmacology},
  number    = {33},
  publisher = {ASPET},
  address   = {Bethesda, Md.},
  issn      = {1521-0111},
  doi       = {10.1124/mol.111.071845},
  pages     = {518 -- 528},
  year      = {2011},
  language  = {en}
}
@article{JordanKruegerWillmesetal.2011,
  author    = {Jordan, Sabine D. and Kr{\"u}ger, Markus and Willmes, Diana M. and Redemann, Nora and Wunderlich, F. Thomas and Br{\"o}nneke, Hella S. and Merkwirth, Carsten and Kashkar, Hamid and Olkkonen, Vesa M. and B{\"o}ttger, Thomas and Braun, Thomas and Seibler, Jost and Br{\"u}ning, Jens C.},
  title     = {Obesity-induced overexpression of miRNA-143 inhibits insulin-stimulated AKT activation and impairs glucose metabolism},
  series = {Nature Cell Biology},
  volume    = {13},
  journal   = {Nature Cell Biology},
  number    = {4},
  publisher = {Nature},
  address   = {New York},
  issn      = {1465-7392},
  doi       = {10.1038/ncb2211},
  pages     = {434 -- 446},
  year      = {2011},
  abstract  = {The contribution of altered post-transcriptional gene silencing to the development of insulin resistance and type 2 diabetes mellitus so far remains elusive. Here, we demonstrate that expression of microRNA (miR)-143 and 145 is upregulated in the liver of genetic and dietary mouse models of obesity. Induced transgenic overexpression of miR-143, but not miR-145, impairs insulin-stimulated AKT activation and glucose homeostasis. Conversely, mice deficient for the miR-143-145 cluster are protected from the development of obesity-associated insulin resistance. Quantitative-mass-spectrometry-based analysis of hepatic protein expression in miR-143-overexpressing mice revealed miR-143-dependent downregulation of oxysterol-binding-protein-related protein (ORP) 8. Reduced ORP8 expression in cultured liver cells impairs the ability of insulin to induce AKT activation, revealing an ORP8-dependent mechanism of AKT regulation. Our experiments provide direct evidence that dysregulated post-transcriptional gene silencing contributes to the development of obesity-induced insulin resistance, and characterize the miR-143-ORP8 pathway as a potential target for the treatment of obesity-associated diabetes.},
  language  = {en}
}
@incollection{SeiblerSchwenk2010,
  author    = {Seibler, Jost and Schwenk, Frieder},
  title     = {Transgenic RNAi Applications in the Mouse},
  series = {Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition},
  booktitle = {Methods in Enzymology : Guide to Techniques in Mouse Development, Part B: Mouse Molecular Genetics. 2nd Edition},
  publisher = {Elsevier},
  address   = {Amsterdam},
  isbn      = {978-0-12-384880-2},
  pages     = {367 -- 386},
  year      = {2010},
  language  = {en}
}
@article{GlaserLubitzLovelandetal.2009,
  author    = {Glaser, Stefan and Lubitz, Sandra and Loveland, Kate L. and Ohbo, Kazu and Robb, Lorraine and Schwenk, Frieder and Seibler, Jost and Roellig, Daniela and Kranz, Andrea and Anastassiadis, Konstantinos and Stewart, A. Francis},
  title     = {The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis},
  series = {Epigenetics \& Chromatin},
  volume    = {2},
  journal   = {Epigenetics \& Chromatin},
  number    = {5},
  issn      = {1756-8935},
  doi       = {10.1186/1756-8935-2-5},
  pages     = {1 -- 16},
  year      = {2009},
  language  = {en}
}
@article{KotnikPopovaTodirasetal.2009,
  author    = {Kotnik, Katarina and Popova, Elena and Todiras, Mihail and Mori, Marcelo A. and Alenina, Natalia and Seibler, Jost and Bader, Michael},
  title     = {Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown},
  series = {Plos One},
  volume    = {4},
  journal   = {Plos One},
  number    = {4},
  publisher = {Plos},
  address   = {San Francisco, California, US},
  doi       = {10.1371/journal.pone.0005124},
  pages     = {e5124},
  year      = {2009},
  language  = {en}
}
@article{HeroldBrandtSeibleretal.2008,
  author    = {Herold, Marco J. and Brandt, Jens van den and Seibler, Jost and Reichardt, Holger M.},
  title     = {Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats},
  series = {Proceedings of the National Academy of Sciences of the United States of America},
  volume    = {105},
  journal   = {Proceedings of the National Academy of Sciences of the United States of America},
  number    = {47},
  issn      = {1091-6490},
  doi       = {10.1073/pnas.0806213105},
  pages     = {18507 -- 18512},
  year      = {2008},
  language  = {en}
}
@article{KochWunderlichSeibleretal.2008,
  author    = {Koch, Linda and Wunderlich, F. Thomas and Seibler, Jost and K{\"o}nner, A. Christine and Hampel, Brigitte and Irlenbusch, Sigrid and Brabant, Georg and Kahn, C. Ronald and Schwenk, Frieder and Br{\"u}ning, Jens C.},
  title     = {Central insulin action regulates peripheral glucose and fat metabolism in mice},
  series = {The Journal of Clinical Investigation (JCI)},
  volume    = {118},
  journal   = {The Journal of Clinical Investigation (JCI)},
  number    = {6},
  issn      = {1558-8238},
  doi       = {10.1172/JCI31073},
  pages     = {2132 -- 2147},
  year      = {2008},
  language  = {en}
}
@article{ChristophBahrenbergVryetal.2008,
  author    = {Christoph, Thomas and Bahrenberg, Gregor and Vry, Jean de and Englberger, Werner and Erdmann, Volker A. and Frech, Moritz and K{\"o}gel, Babette and R{\"o}hl, Thomas and Schiene, Klaus and Schr{\"o}der, Wolfgang and Seibler, Jost and Kurreck, Jens},
  title     = {Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice},
  series = {Molecular and Cellular Neuroscience},
  volume    = {37},
  journal   = {Molecular and Cellular Neuroscience},
  number    = {3},
  issn      = {1044-7431},
  doi       = {10.1016/j.mcn.2007.12.006},
  pages     = {579 -- 589},
  year      = {2008},
  language  = {en}
}
@article{SeiblerKleinriddersKueterLuksetal.2007,
  author    = {Seibler, Jost and Kleinridders, Andre and K{\"u}ter-Luks, Birgit and Niehaves, Sandra and Br{\"u}ning, Jens C. and Schwenk, Frieder},
  title     = {Reversible gene knockdown in mice using a tight, inducible shRNA expression system},
  series = {Nucleic Acids Research},
  volume    = {35},
  journal   = {Nucleic Acids Research},
  number    = {7},
  issn      = {1362-4962},
  doi       = {10.1093/nar/gkm122},
  pages     = {e54},
  year      = {2007},
  language  = {en}
}
@article{ScheerSnaithWolfetal.2013,
  author    = {Scheer, Nico and Snaith, Mike and Wolf, C. Roland and Seibler, Jost},
  title     = {Generation and utility of genetically humanized mouse models},
  series = {Drug Discovery Today},
  volume    = {Vol 18},
  journal   = {Drug Discovery Today},
  number    = {23-24},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1359-6446},
  doi       = {10.1016/j.drudis.2013.07.007},
  pages     = {1200 -- 1211},
  year      = {2013},
  language  = {en}
}
@article{BouwmanGuldenHeijdenetal.2013,
  author    = {Bouwman, Peter and Gulden, Hanneke van der and Heijden, Ingrid van der and Drost, Rinske and Klijn, Christiaan N. and Prasetyanti, Pramudita and Pieterse, Mark and Wientjens, Ellen and Seibler, Jost and Hogervorst, Frank B. L. and Jonkers, Jos},
  title     = {A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants},
  series = {Cancer Discovery},
  journal   = {Cancer Discovery},
  number    = {3},
  issn      = {2159-8290},
  doi       = {10.1158/2159-8290.CD-13-0094},
  pages     = {1142 -- 1152},
  year      = {2013},
  language  = {en}
}
@article{MichalakNacerddinePietersenetal.2013,
  author    = {Michalak, Ewa Malgorzata and Nacerddine, Karim and Pietersen, Alexandra and Beuger, Vincent and Pawlitzky, Inka and Cornelissen-Steijger, Paulien and Wientjens, Ellen and Tanger, Ellen and Seibler, Jost and Lohuizen, Maarten van and Jonkers, Jos},
  title     = {Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool},
  series = {Stem Cells},
  volume    = {Vol 31},
  journal   = {Stem Cells},
  number    = {9},
  publisher = {Oxford University Press},
  address   = {Oxford},
  issn      = {1549-4918},
  doi       = {10.1002/stem.1437},
  pages     = {1910 -- 1920},
  year      = {2013},
  language  = {en}
}
@article{GebeshuberKornauthDongetal.2013,
  author    = {Gebeshuber, Christoph A. and Kornauth, Christoph and Dong, Lihua and Sierig, Ralph and Seibler, Jost and Reiss, Martina and Tauber, Stefanie and Bilban, Martin and Wang, Shijun and Kain, Renate and B{\"o}hmig, Georg A. and Moeller, Marcus J. and Gr{\"o}ne, Hermann-Josef and Englert, Christoph and Martinez, Javier and Kerjaschki, Dontscho},
  title     = {Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1},
  series = {Nature Medicine},
  volume    = {19},
  journal   = {Nature Medicine},
  number    = {4},
  issn      = {1078-8956},
  doi       = {10.1038/nm.3142},
  pages     = {481 -- 487},
  year      = {2013},
  language  = {en}
}
@article{KornfeldBaitzelKoenneretal.2013,
  author    = {Kornfeld, Jan-Wilhelm and Baitzel, Catherina and K{\"o}nner, A. Christine and Nicholls, Hayley T. and Vogt, Merly C. and Herrmanns, Karolin and Scheja, Ludger and Haumaitre, C{\´e}cile and Wolf, Anna M. and Knippschild, Uwe and Seibler, Jost and Cereghini, Silvia and Heeren, Joerg and Stoffel, Markus and Br{\"u}ning, Jens C.},
  title     = {Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b},
  series = {Nature},
  volume    = {494},
  journal   = {Nature},
  number    = {7435},
  publisher = {Springer Nature},
  address   = {Cham},
  isbn      = {0028-0836},
  doi       = {10.1038/nature11793},
  pages     = {111 -- 115},
  year      = {2013},
  language  = {en}
}
@article{InagakiSleddensLinkelsWassenaaretal.2011,
  author    = {Inagaki, Akiko and Sleddens-Linkels, Esther and Wassenaar, Evelyne and Ooms, Marja and Cappellen, Wiggert A. van and Hoeijmakers, Jan H. J. and Seibler, Jost and Vogt, Thomas F. and Shin, Myung K. and Grootegoed, J. Anton and Baarends, Willy M.},
  title     = {Meiotic functions of RAD18},
  series = {Journal of Cell Science},
  volume    = {124},
  journal   = {Journal of Cell Science},
  number    = {16},
  publisher = {Company of Biologists Limited},
  address   = {Cambridge},
  issn      = {1477-9137},
  doi       = {10.1242/jcs.081968},
  pages     = {2837 -- 2850},
  year      = {2011},
  language  = {en}
}
@article{SchiffelsSelmer2015,
  author    = {Schiffels, Johannes and Selmer, Thorsten},
  title     = {A flexible toolbox to study protein-assisted metalloenzyme assembly in vitro},
  series = {Biotechnology and Bioengineering},
  volume    = {112},
  journal   = {Biotechnology and Bioengineering},
  number    = {11},
  publisher = {Wiley},
  address   = {Weinheim},
  issn      = {1097-0290},
  doi       = {10.1002/bit.25658},
  pages     = {2360 -- 2372},
  year      = {2015},
  language  = {en}
}
@article{VoigtAlbrechtSieversetal.2015,
  author    = {Voigt, Birgit and Albrecht, Dirk and Sievers, Susanne and Becher, D{\"o}rte and Bongaerts, Johannes and Evers, Stefan and Schweder, Thomas and Maurer, Karl-Heinz and Hecker, Michael},
  title     = {High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium},
  series = {Proteomics},
  volume    = {15},
  journal   = {Proteomics},
  number    = {15},
  publisher = {Wiley},
  address   = {Weinheim},
  issn      = {1615-9861},
  doi       = {10.1002/pmic.201400504},
  pages     = {2629 -- 2633},
  year      = {2015},
  language  = {en}
}
@article{MolinnusBaeckerSiegertetal.2015,
  author    = {Molinnus, Denise and B{\"a}cker, Matthias and Siegert, Petra and Willenberg, H. and Poghossian, Arshak and Keusgen, M. and Sch{\"o}ning, Michael Josef},
  title     = {Detection of Adrenaline Based on Substrate Recycling Amplification},
  series = {Procedia Engineering},
  volume    = {120},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2015.08.708},
  pages     = {540 -- 543},
  year      = {2015},
  abstract  = {An amperometric enzyme biosensor has been applied for the detection of adrenaline. The adrenaline biosensor has been prepared by modification of an oxygen electrode with the enzyme laccase that operates at a broad pH range between pH 3.5 to pH 8. The enzyme molecules were immobilized via cross-linking with glutaraldehyde. The sensitivity of the developed adrenaline biosensor in different pH buffer solutions has been studied.},
  language  = {en}
}
@article{PilasMarianoKeusgenetal.2015,
  author    = {Pilas, Johanna and Mariano, K. and Keusgen, M. and Selmer, Thorsten and Sch{\"o}ning, Michael Josef},
  title     = {Optimization of an Enzyme-based Multi-parameter Biosensor for Monitoring Biogas Processes},
  series = {Procedia Engineering},
  volume    = {120},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2015.08.702},
  pages     = {532 -- 535},
  year      = {2015},
  language  = {en}
}
@article{PlumMaHampeletal.2006,
  author    = {Plum, Leona and Ma, Xiaosong and Hampel, Brigitte and Balthasar, Nina and Coppari, Roberto and M{\"u}nzberg, Heike and Shanabrough, Marya and Burdakov, Denis and Rother, Eva and Janoschek, Ruth and Alber, Jens and Belgardt, Bengt F. and Koch, Linda and Seibler, Jost and Schenk, Frieder and Fekete, Csaba and Suzuki, Akira and Mak, Tak W. and Krone, Wilhelm and Horvath, Tamas L. and Ashcroft, Frances M. and Br{\"u}ning, Jens C.},
  title     = {Enhanced PIP3 signaling in POMC neurons causes KATP channel activation and leads to diet-sensitive obesity},
  series = {The Journal of Clinical Investigation (JCI)},
  volume    = {116},
  journal   = {The Journal of Clinical Investigation (JCI)},
  number    = {7},
  issn      = {1558-8238},
  doi       = {10.1172/JCI27123},
  pages     = {1886 -- 1901},
  year      = {2006},
  language  = {en}
}
@article{SeiblerKueterLuksKernetal.2005,
  author    = {Seibler, Jost and K{\"u}ter-Luks, Birgit and Kern, Heidrun and Streu, Sandra and Plum, Leona and Maurer, Jan and K{\"u}hn, Ralf and Br{\"u}ning, Jens C. and Schwenk, Frieder},
  title     = {Single copy shRNA configuration for ubiquitous gene knockdown in mice},
  series = {Nucleic Acids Research},
  volume    = {33},
  journal   = {Nucleic Acids Research},
  number    = {7},
  issn      = {1362-4962},
  doi       = {10.1093/nar/gni065},
  pages     = {e67},
  year      = {2005},
  language  = {en}
}
@article{SeiblerZevnikKueterLuksetal.2003,
  author    = {Seibler, Jost and Zevnik, Branko and K{\"u}ter-Luks, Birgit and Andreas, Susanne and Kern, Heidrun and Hennek, Thomas and Rode, Anja and Heimann, Cornelia and Faust, Nicole and Kauselmann, Gunther and Schoor, Michael and Jaenisch, Rudolf and Rajewsky, Klaus and K{\"u}hn, Ralf and Schwenk, Frieder},
  title     = {Rapid generation of inducible mouse mutants},
  series = {Nucleic Acids Research},
  volume    = {33},
  journal   = {Nucleic Acids Research},
  number    = {4},
  issn      = {1362-4962},
  doi       = {10.1093/nar/gng012},
  pages     = {e12},
  year      = {2003},
  language  = {en}
}
@article{GoetzeBaerWinkelmannetal.2005,
  author    = {Goetze, Sandra and Baer, Alexandra and Winkelmann, Silke and Nehlsen, Kristina and Seibler, Jost and Maass, Karin and Bode, J{\"u}rgen},
  title     = {Performance of genomic bordering elements at predefined genomic loci},
  series = {Molecular and Cellular Biology},
  volume    = {25},
  journal   = {Molecular and Cellular Biology},
  number    = {6},
  issn      = {1098-5549},
  doi       = {10.1128/MCB.25.6.2260-2272.2005},
  pages     = {2260 -- 2272},
  year      = {2005},
  language  = {en}
}