@article{ScheerSnaithWolfetal.2013, author = {Scheer, Nico and Snaith, Mike and Wolf, C. Roland and Seibler, Jost}, title = {Generation and utility of genetically humanized mouse models}, series = {Drug Discovery Today}, volume = {Vol 18}, journal = {Drug Discovery Today}, number = {23-24}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1359-6446}, doi = {10.1016/j.drudis.2013.07.007}, pages = {1200 -- 1211}, year = {2013}, language = {en} } @article{LassonczykAlailyHuthetal.1990, author = {Lassonczyk, Beate and Alaily, F. and Huth, A. and Gensior, A.}, title = {Genesis of soils in the arid part of northeast Somalia / F. Alaily, B. Lassonczyk, A.Huth and A. Gensior}, series = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, journal = {Berliner geowissenschaftliche Abhandlungen / Reihe A, Geologie und Pal{\"a}ontologie / hrsg. von d. Geowissenschaftlichen Instituten der Freien u. d. Technischen Universit{\"a}t Berlin. 120 A (1990)}, isbn = {0172-8784}, pages = {695 -- 711}, year = {1990}, language = {en} } @article{BorgmeierBongaertsMeinhardt2012, author = {Borgmeier, Claudia and Bongaerts, Johannes and Meinhardt, Friedhelm}, title = {Genetic analysis of the Bacillus licheniformis degSU operon and the impact of regulatory mutations on protease production}, series = {Journal of biotechnology}, volume = {159}, journal = {Journal of biotechnology}, number = {1-2}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2012.02.011}, pages = {12 -- 20}, year = {2012}, abstract = {Disruption experiments targeted at the Bacillus licheniformis degSU operon and GFP-reporter analysis provided evidence for promoter activity immediately upstream of degU. pMutin mediated concomitant introduction of the degU32 allele - known to cause hypersecretion in Bacillus subtilis - resulted in a marked increase in protease activity. Application of 5-fluorouracil based counterselection through establishment of a phosphoribosyltransferase deficient Δupp strain eventually facilitated the marker-free introduction of degU32 leading to further protease enhancement achieving levels as for hypersecreting wild strains in which degU was overexpressed. Surprisingly, deletion of rapG - known to interfere with DegU DNA-binding in B. subtilis - did not enhance protease production neither in the wild type nor in the degU32 strain. The combination of degU32 and Δupp counterselection in the type strain is not only equally effective as in hypersecreting wild strains with respect to protease production but furthermore facilitates genetic strain improvement aiming at biological containment and effectiveness of biotechnological processes.}, language = {en} } @article{DeppeKlatteBongaertsetal.2011, author = {Deppe, Veronika Maria and Klatte, Stephanie and Bongaerts, Johannes and Maurer, Karl-Heinz and O'Connell, Timothy and Meinhardt, Friedhelm}, title = {Genetic control of Amadori product degradation in Bacillus subtilis via regulation of frlBONMD expression by FrlR}, series = {Applied and environmental microbiology}, volume = {Vol. 77}, journal = {Applied and environmental microbiology}, number = {No. 9}, publisher = {American Society of Mechanical Engineers (ASME)}, address = {New York}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, pages = {2839 -- 2846}, year = {2011}, language = {en} } @article{MedlinLangeBaumann1994, author = {Medlin, L. K. and Lange, M. and Baumann, Marcus}, title = {Genetic differentiation among three colony-forming species of Phaeocystis : further evidence for the phylogeny of the Prymnesiophyta}, series = {Phycologia}, volume = {Vol. 33}, journal = {Phycologia}, number = {Iss. 3}, issn = {0031-8884}, pages = {199 -- 212}, year = {1994}, language = {en} } @incollection{SamuelssonScheerWilsonetal.2017, author = {Samuelsson, K. and Scheer, Nico and Wilson, I. and Wolf, C.R. and Henderson, C.J.}, title = {Genetically Humanized Animal Models}, series = {Comprehensive Medicinal Chemistry III. 3rd Edition}, booktitle = {Comprehensive Medicinal Chemistry III. 3rd Edition}, editor = {Chackalamannil, Samuel}, publisher = {Elsevier}, address = {Saint Louis}, isbn = {978-0-12-803201-5}, doi = {10.1016/B978-0-12-409547-2.12376-5}, pages = {130 -- 149}, year = {2017}, abstract = {Genetically humanized mice for proteins involved in drug metabolism and toxicity and mice engrafted with human hepatocytes are emerging as promising in vivo models for improved prediction of the pharmacokinetic, drug-drug interaction, and safety characteristics of compounds in humans. This is an overview on the genetically humanized and chimeric liver-humanized mouse models, which are illustrated with examples of their utility in drug metabolism and toxicity studies. The models are compared to give guidance for selection of the most appropriate model by highlighting advantages and disadvantages to be carefully considered when used for studies in drug discovery and development.}, language = {en} } @article{ScheerWolf2014, author = {Scheer, Nico and Wolf, C. Roland}, title = {Genetically humanized mouse models of drug metabolizing enzymes and transporters and their applications}, series = {Xenobiotica}, volume = {44}, journal = {Xenobiotica}, number = {2}, publisher = {Taylor \& Francis}, address = {Abingdon}, issn = {1366-5928}, doi = {10.3109/00498254.2013.815831}, pages = {96 -- 108}, year = {2014}, abstract = {1. Drug metabolizing enzymes and transporters play important roles in the absorption, metabolism, tissue distribution and excretion of various compounds and their metabolites and thus can significantly affect their efficacy and safety. Furthermore, they can be involved in drug-drug interactions which can result in adverse responses, life-threatening toxicity or impaired efficacy. Significant species differences in the interaction of compounds with drug metabolizing enzymes and transporters have been described. 2. In order to overcome the limitation of animal models in accurately predicting human responses, a large variety of mouse models humanized for drug metabolizing enzymes and to a lesser extent drug transporters have been created. 3. This review summarizes the literature describing these mouse models and their key applications in studying the role of drug metabolizing enzymes and transporters in drug bioavailability, tissue distribution, clearance and drug-drug interactions as well as in human metabolite testing and risk assessment. 4. Though such humanized mouse models have certain limitations, there is great potential for their use in basic research and for testing and development of new medicines. These limitations and future potentials will be discussed.}, language = {en} } @article{WallerBraunHojdisetal.2007, author = {Waller, Mark P. and Braun, Heiko and Hojdis, Nils and B{\"u}hl, Michael}, title = {Geometries of Second-Row Transition-Metal Complexes from Density-Functional Theory}, series = {Journal of Chemical Theory and Computation}, volume = {3}, journal = {Journal of Chemical Theory and Computation}, number = {6}, issn = {1549-9626}, doi = {10.1021/ct700178y}, pages = {2234 -- 2242}, year = {2007}, language = {en} } @article{SelmerSommerladeIngendohetal.1994, author = {Selmer, Thorsten and Sommerlade, Hans-J{\"o}rg and Ingendoh, Arnd and Gieselmann, Volkmar}, title = {Glycosylation and phosphorylation of arylsulfatase A / Sommerlade, Hans-J{\"o}rg. ; Selmer, Thomas. ; Ingendoh, Arnd ; Gieselmann, Volkmar ; Figura, Kurt von ; Neifer, Klaus ; Schmidt, Bernhard}, series = {Journal of Biological Chemistry. 269 (1994), H. 33}, journal = {Journal of Biological Chemistry. 269 (1994), H. 33}, isbn = {1083-351X}, pages = {20977 -- 20981}, year = {1994}, language = {en} } @article{BiselliLuellauWandrey1994, author = {Biselli, Manfred and L{\"u}llau, E. and Wandrey, Christian}, title = {Growth and metabolism of CHO-cells in porous glass carriers / L{\"u}llau, E. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : products of today, prospects for tomorrow ; ESACT, European Society for Animal Cell Technology, the 12th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750618450}, pages = {252 -- 255}, year = {1994}, language = {en} } @incollection{DuongSeifarthTemizArtmannetal.2018, author = {Duong, Minh Tuan and Seifarth, Volker and Temiz Artmann, Ayseg{\"u}l and Artmann, Gerhard and Staat, Manfred}, title = {Growth Modelling Promoting Mechanical Stimulation of Smooth Muscle Cells of Porcine Tubular Organs in a Fibrin-PVDF Scaffold}, series = {Biological, Physical and Technical Basics of Cell Engineering}, booktitle = {Biological, Physical and Technical Basics of Cell Engineering}, editor = {Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l and Zhubanova, Azhar A. and Digel, Ilya}, publisher = {Springer}, address = {Singapore}, isbn = {978-981-10-7904-7}, doi = {10.1007/978-981-10-7904-7_9}, pages = {209 -- 232}, year = {2018}, abstract = {Reconstructive surgery and tissue replacements like ureters or bladders reconstruction have been recently studied, taking into account growth and remodelling of cells since living cells are capable of growing, adapting, remodelling or degrading and restoring in order to deform and respond to stimuli. Hence, shapes of ureters or bladders and their microstructure change during growth and these changes strongly depend on external stimuli such as training. We present the mechanical stimulation of smooth muscle cells in a tubular fibrin-PVDFA scaffold and the modelling of the growth of tissue by stimuli. To this end, mechanotransduction was performed with a kyphoplasty balloon catheter that was guided through the lumen of the tubular structure. The bursting pressure was examined to compare the stability of the incubated tissue constructs. The results showed the significant changes on tissues with training by increasing the burst pressure as a characteristic mechanical property and the smooth muscle cells were more oriented with uniformly higher density. Besides, the computational growth models also exhibited the accurate tendencies of growth of the cells under different external stimuli. Such models may lead to design standards for the better layered tissue structure in reconstructing of tubular organs characterized as composite materials such as intestines, ureters and arteries.}, language = {en} } @article{WackwitzBongaertsGoodmanetal.1999, author = {Wackwitz, B. and Bongaerts, Johannes and Goodman, S. D. and Unden, Gottfried}, title = {Growth phase-dependent regulation of nuoA-N expression in Escherichia coli K-12 by the Fis protein: upstream binding sites and bioenergetic significance}, series = {Molecular and general genetics : MGG}, volume = {Vol. 262}, journal = {Molecular and general genetics : MGG}, number = {Iss. 4 - 5}, issn = {1617-4623 (E-Journal); 1617-4615 (Print)}, pages = {876 -- 883}, year = {1999}, language = {en} } @article{WagnerMolinaYoshinobuetal.2007, author = {Wagner, Torsten and Molina, Roberto and Yoshinobu, Tatsuo and Kloock, Joachim P. and Biselli, Manfred and Canzoneri, Michele and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef}, title = {Handheld multi-channel LAPS device as a transducer platform for possible biological and chemical multi-sensor applications}, series = {Electrochimica Acta. 53 (2007), H. 2}, journal = {Electrochimica Acta. 53 (2007), H. 2}, isbn = {0013-4686}, pages = {305 -- 311}, year = {2007}, language = {en} } @article{HansScheerRiedletal.2004, author = {Hans, Stefan and Scheer, Nico and Riedl, Iris and Weiz{\"a}cker, Elisabeth von and Blader, Patrick and Campos-Ortega, Jos{\´e} A.}, title = {her3, a zebrafish member of the hairy-E(spl) family, is repressed by Notch signalling}, series = {Development}, volume = {131}, journal = {Development}, number = {12}, issn = {1477-9129}, doi = {10.1242/dev.01167}, pages = {2957 -- 2969}, year = {2004}, language = {en} } @article{PrielmeierSpeedyLuedemann1987, author = {Prielmeier, Franz and Speedy, R. J. and L{\"u}demann, H.-D.}, title = {High Pressure NMR Self-Diffusion Studies on Supercooled Water}, series = {High Pressure Science and Technology Proceeding XI AIRAPT, Kiew. 1}, journal = {High Pressure Science and Technology Proceeding XI AIRAPT, Kiew. 1}, pages = {75}, year = {1987}, language = {en} } @article{PrielmeierLangRadkowitschetal.1987, author = {Prielmeier, Franz and Lang, E. W. and Radkowitsch, H. and L{\"u}demann, H. D.}, title = {High Pressure NMR Study of the Molecular Dynamics of Liquid fluoroform and deutero-fluoroform / Lang, E. W. ; Prielmeier, F. X. ; Radkowitsch, H. ; L{\"u}demann, H. D.}, series = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 91 (1987), H. 10}, journal = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 91 (1987), H. 10}, isbn = {0005-9021}, pages = {1025 -- 1033}, year = {1987}, language = {en} } @article{PrielmeierLangRadkowitschetal.1987, author = {Prielmeier, Franz and Lang, E. W. and Radkowitsch, H. and L{\"u}demann, H.-D.}, title = {High Pressure NMR Study of the Molecular Dynamics of Liquid methyl fluoride and deutero-methyl fluoride / Lang, E. W. ; Prielmeier, F. X. ; Radkowitsch, H. ; L{\"u}demann, H. D.}, series = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 91 (1987), H. 10}, journal = {Berichte der Bunsen-Gesellschaft f{\"u}r Physikalische Chemie. 91 (1987), H. 10}, isbn = {0005-9021}, pages = {1017 -- 1025}, year = {1987}, language = {en} } @article{HeineHerrmannSelmeretal.2014, author = {Heine, A. and Herrmann, G. and Selmer, Thorsten and Terwesten, F. and Buckel, W. and Reuter, K.}, title = {High resolution crystal structure of clostridium propionicum β-Alanyl-CoA:Ammonia Lyase, a new member of the "Hot Dog Fold" protein superfamily}, series = {Proteins}, volume = {82}, journal = {Proteins}, number = {9}, publisher = {Wiley-Liss}, address = {New York}, issn = {1097-0134 (E-Journal); 0887-3585 (Print)}, doi = {10.1002/prot.24557}, pages = {2041 -- 2053}, year = {2014}, abstract = {Clostridium propionicum is the only organism known to ferment β-alanine, a constituent of coenzyme A (CoA) and the phosphopantetheinyl prosthetic group of holo-acyl carrier protein. The first step in the fermentation is a CoA-transfer to β-alanine. Subsequently, the resulting β-alanyl-CoA is deaminated by the enzyme β-alanyl-CoA:ammonia lyase (Acl) to reversibly form ammonia and acrylyl-CoA. We have determined the crystal structure of Acl in its apo-form at a resolution of 0.97 {\AA} as well as in complex with CoA at a resolution of 1.59 {\AA}. The structures reveal that the enyzme belongs to a superfamily of proteins exhibiting a so called "hot dog fold" which is characterized by a five-stranded antiparallel β-sheet with a long α-helix packed against it. The functional unit of all "hot dog fold" proteins is a homodimer containing two equivalent substrate binding sites which are established by the dimer interface. In the case of Acl, three functional dimers combine to a homohexamer strongly resembling the homohexamer formed by YciA-like acyl-CoA thioesterases. Here, we propose an enzymatic mechanism based on the crystal structure of the Acl·CoA complex and molecular docking. Proteins 2014; 82:2041-2053. © 2014 Wiley Periodicals, Inc.}, language = {en} } @article{VoigtAlbrechtSieversetal.2015, author = {Voigt, Birgit and Albrecht, Dirk and Sievers, Susanne and Becher, D{\"o}rte and Bongaerts, Johannes and Evers, Stefan and Schweder, Thomas and Maurer, Karl-Heinz and Hecker, Michael}, title = {High-resolution proteome maps of Bacillus licheniformis cells growing in minimal medium}, series = {Proteomics}, volume = {15}, journal = {Proteomics}, number = {15}, publisher = {Wiley}, address = {Weinheim}, issn = {1615-9861}, doi = {10.1002/pmic.201400504}, pages = {2629 -- 2633}, year = {2015}, language = {en} } @article{RossPlummerRodeetal.2010, author = {Ross, Jillian and Plummer, Simon M. and Rode, Anja and Scheer, Nico and Bower, Conrad C. and Vogel, Ortwin and Henderson, Colin J. and Wolf, C. Roland and Elcombe, Clifford R.}, title = {Human constitutive androstane receptor (CAR) and pregnane X receptor (PXR) support the hypertrophic but not the hyperplastic response to the murine nongenotoxic hepatocarcinogens phenobarbital and chlordane in vivo}, series = {Toxicological Sciences}, volume = {116}, journal = {Toxicological Sciences}, number = {2}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1096-0929}, doi = {10.1093/toxsci/kfq118}, pages = {452 -- 466}, year = {2010}, abstract = {Mouse nongenotoxic hepatocarcinogens phenobarbital (PB) and chlordane induce hepatomegaly characterized by hypertrophy and hyperplasia. Increased cell proliferation is implicated in the mechanism of tumor induction. The relevance of these tumors to human health is unclear. The xenoreceptors, constitutive androstane receptors (CARs), and pregnane X receptor (PXR) play key roles in these processes. Novel "humanized" and knockout models for both receptors were developed to investigate potential species differences in hepatomegaly. The effects of PB (80 mg/kg/4 days) and chlordane (10 mg/kg/4 days) were investigated in double humanized PXR and CAR (huPXR/huCAR), double knockout PXR and CAR (PXRKO/CARKO), and wild-type (WT) C57BL/6J mice. In WT mice, both compounds caused increased liver weight, hepatocellular hypertrophy, and cell proliferation. Both compounds caused alterations to a number of cell cycle genes consistent with induction of cell proliferation in WT mice. However, these gene expression changes did not occur in PXRKO/CARKO or huPXR/huCAR mice. Liver hypertrophy without hyperplasia was demonstrated in the huPXR/huCAR animals in response to both compounds. Induction of the CAR and PXR target genes, Cyp2b10 and Cyp3a11, was observed in both WT and huPXR/huCAR mouse lines following treatment with PB or chlordane. In the PXRKO/CARKO mice, neither liver growth nor induction of Cyp2b10 and Cyp3a11 was seen following PB or chlordane treatment, indicating that these effects are CAR/PXR dependent. These data suggest that the human receptors are able to support the chemically induced hypertrophic responses but not the hyperplastic (cell proliferation) responses. At this time, we cannot be certain that hCAR and hPXR when expressed in the mouse can function exactly as the genes do when they are expressed in human cells. However, all parameters investigated to date suggest that much of their functionality is maintained.}, language = {en} } @article{WhiteheadOehlschlaegerAlmajhdietal.2014, author = {Whitehead, Mark and {\"O}hlschl{\"a}ger, Peter and Almajhdi, Fahad N. and Alloza, Leonor and Marz{\´a}bal, Pablo and Meyers, Ann E. and Hitzeroth, Inga I. and Rybicki, Edward P.}, title = {Human papillomavirus (HPV) type 16 E7 protein bodies cause tumour regression in mice}, series = {BMC cancer}, journal = {BMC cancer}, number = {14:367}, publisher = {BioMed Central}, address = {London}, issn = {1471-2407}, doi = {10.1186/1471-2407-14-367}, pages = {1 -- 15}, year = {2014}, language = {en} } @article{OehlschlaegerOsenDelletal.2003, author = {{\"O}hlschl{\"a}ger, Peter and Osen, Wolfram and Dell, Kerstin and Faath, Stefan}, title = {Human papillomavirus type 16 L1 capsomeres induce L1-specific cytotoxic T lymphocytes and tumor regression in C57BL/6 mice / {\"O}hlschl{\"a}ger, Peter ; Osen, Wolfram ; Dell, Kerstin ; Faath, Stefan ; Garcea Robert L: ; Jochmus, Ingrid ; M{\"u}ller, Martin, Pawlita,}, series = {Journal of Virology. 77 (2003), H. 8}, journal = {Journal of Virology. 77 (2003), H. 8}, isbn = {1098-5514}, pages = {4635 -- 4645}, year = {2003}, language = {en} } @article{DanhoNaithaniSasakietal.1980, author = {Danho, Waleed and Naithani, Vinod K. and Sasaki, Andr{\´e} N. and F{\"o}hles, Joseph and Berndt, Heinz and [u.a.],}, title = {Human proinsulin, VII : synthesis of two protected peptides corresponding to the sequences 1—45 and 46—86 of the prohormone}, series = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, volume = {361}, journal = {Hoppe-Seyler's Zeitschrift f{\"u}r physiologische Chemie}, number = {1}, issn = {1437-4315}, doi = {10.1515/bchm2.1980.361.1.857}, pages = {857 -- 863}, year = {1980}, language = {en} } @inproceedings{KazukiKobayashiHirabayashietal.2019, author = {Kazuki, Yasuhiro and Kobayashi, Kaoru and Hirabayashi, Masumi and Abe, Satoshi and Kajitani, Naoyo and Kazuki, Kanoko and Takehara, Shoko and Takiguchi, Masato and Satoh, Daisuke and Kuze, Jiro and Sakuma, Tetsushi and Kaneko, Takehito and Mashimo, Tomoji and Osamura, Minori and Hashimoto, Mari and Wakatsuki, Riko and Hirashima, Rika and Fujiwara, Ryoichi and Deguchi, Tsuneo and Kurihara, Atsushi and Tsukazaki, Yasuko and Senda, Naoto and Yamamoto, Takashi and Scheer, Nico and Oshimura, Mitsuo}, title = {Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism}, series = {PNAS Proceedings of the National Academy of Sciences of the United States of America}, volume = {116}, booktitle = {PNAS Proceedings of the National Academy of Sciences of the United States of America}, number = {8}, issn = {1091-6490}, doi = {10.1073/pnas.1808255116}, pages = {3072 -- 3081}, year = {2019}, language = {en} } @article{BreuerRaueStrobeletal.2016, author = {Breuer, Lars and Raue, Markus and Strobel, M. and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, R. and Wagner, Torsten}, title = {Hydrogels with incorporated graphene oxide as light-addressable actuator materials for cell culture environments in lab-on-chip systems}, series = {Physica status solidi (a)}, volume = {213}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300}, doi = {10.1002/pssa.201533056}, pages = {1520 -- 1525}, year = {2016}, abstract = {Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.}, language = {en} } @article{LempiaeinenCouttetBolognanietal.2012, author = {Lempi{\"a}inen, Harri and Couttet, Philippe and Bolognani, Federico and M{\"u}ller, Arne and Dubost, Val{\´e}rie and Luisier, Rapha{\"e}lle and Rio-Espinola, Alberto del and Vitry, Veronique and Unterberger, Elif B. and Thomson, John P. and Treindl, Fridolin and Metzger, Ute and Wrzodek, Clemens and Hahne, Florian and Zollinger, Tulipan and Brasa, Sarah and Kalteis, Magdalena and Marcellin, Magali and Giudicelli, Fanny and Braeuning, Albert and Morawiec, Laurent and Zamurovic, Natasa and L{\"a}ngle, Ulrich and Scheer, Nico and Sch{\"u}beler, Dirk and Goodman, Jay and Chibout, Salah-Dine and Marlowe, Jennifer and Theil, Dietlinde and Heard, David J. and Grenet, Olivier and Zell, Andreas and Templin, Markus F. and Meehan, Richard R. and Wolf, Roland C. and Elcombe, Clifford R. and Schwarz, Michael and Moulin, Pierre and Terranova, R{\´e}mi and Moggs, Jonathan G.}, title = {Identification of Dlk1-Dio3 imprinted gene cluster non-coding RNAs as novel candidate biomarkers for liver tumor promotion}, series = {Toxicological Sciences}, volume = {131}, journal = {Toxicological Sciences}, number = {2}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1094-2025}, doi = {10.1093/toxsci/kfs303}, pages = {375 -- 386}, year = {2012}, abstract = {The molecular events during nongenotoxic carcinogenesis and their temporal order are poorly understood but thought to include long-lasting perturbations of gene expression. Here, we have investigated the temporal sequence of molecular and pathological perturbations at early stages of phenobarbital (PB) mediated liver tumor promotion in vivo. Molecular profiling (mRNA, microRNA [miRNA], DNA methylation, and proteins) of mouse liver during 13 weeks of PB treatment revealed progressive increases in hepatic expression of long noncoding RNAs and miRNAs originating from the Dlk1-Dio3 imprinted gene cluster, a locus that has recently been associated with stem cell pluripotency in mice and various neoplasms in humans. PB induction of the Dlk1-Dio3 cluster noncoding RNA (ncRNA) Meg3 was localized to glutamine synthetase-positive hypertrophic perivenous hepatocytes, sug- gesting a role for β-catenin signaling in the dysregulation of Dlk1-Dio3 ncRNAs. The carcinogenic relevance of Dlk1-Dio3 locus ncRNA induction was further supported by in vivo genetic dependence on constitutive androstane receptor and β-catenin pathways. Our data identify Dlk1-Dio3 ncRNAs as novel candidate early biomarkers for mouse liver tumor promotion and provide new opportunities for assessing the carcinogenic potential of novel compounds.}, language = {en} } @inproceedings{KalbeKuropkaMeyerStorketal.1988, author = {Kalbe, Jochen and Kuropka, Rolf and Meyer-Stork, L. Sebastian and Lauter, S. L. and H{\"o}cker, Hartwig and Berndt, Heinz}, title = {Identification of fine animal hair via DNA analysis}, series = {Proceedings of the 1st International Symposium on Specialty Animal Fibers : Aachen, October 26 - 27, 1987 ; [scientific, technological and economical aspects] . - (Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. ; 103)}, booktitle = {Proceedings of the 1st International Symposium on Specialty Animal Fibers : Aachen, October 26 - 27, 1987 ; [scientific, technological and economical aspects] . - (Schriftenreihe des Deutschen Wollforschungsinstitutes an der Technischen Hochschule Aachen e.V. ; 103)}, editor = {K{\"o}rner, Andrea}, publisher = {Dt. Wollforschungsinst.}, address = {Aachen}, issn = {0930-3723}, pages = {221 -- 227}, year = {1988}, language = {en} } @article{Elbers2009, author = {Elbers, Gereon}, title = {Identification of marker peptides in hair for discrimination with regard to species and breed / Koerner, Andrea ; Ring, Maike ; Fabry, Marlies ; Elbers, Gereon ; Moeller, Martin}, series = {Proceedings of the Aachen-Dresden International Textile Conference, 3rd, Aachen, Germany, Nov. 26-27, 2009}, journal = {Proceedings of the Aachen-Dresden International Textile Conference, 3rd, Aachen, Germany, Nov. 26-27, 2009}, publisher = {DWI}, address = {Aachen}, pages = {1 CD-ROM}, year = {2009}, language = {en} } @article{BiselliLuellauDreisbachetal.1992, author = {Biselli, Manfred and L{\"u}llau, E. and Dreisbach, C. and Grogg, A.}, title = {Immobilization of animal cells on chemically modified siran carrier / L{\"u}llau, E. ; Dreisbach, C. ; Grogg, A. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750604212}, pages = {469 -- 475}, year = {1992}, language = {en} } @article{KotterRiekert1983, author = {Kotter, Michael and Riekert, L.}, title = {Impregnation-type catalysts with nonuniform distribution of the active component}, series = {Chemical engineering fundamentals. 2 (1983)}, journal = {Chemical engineering fundamentals. 2 (1983)}, isbn = {0723-0966}, pages = {19 -- 38}, year = {1983}, language = {en} } @article{BiselliNollEisenkraetzeretal.2002, author = {Biselli, Manfred and Noll, Thomas and Eisenkr{\"a}tzer, Detlef and Kiesewetter, Andr{\´e}}, title = {Improved product formation in high density Chinese hamster ovary cell cultures transfected at confluency / Thomas Noll, Detlef Eisenkr{\"a}tzer, Andr{\´e} Kiesewetter, Andr{\´e} Dinter, Steffen Zeng, Christian Wandrey, Manfred Biselli und Eric G. Berger}, series = {Biotechnology Letters. 24 (2002), H. 11}, journal = {Biotechnology Letters. 24 (2002), H. 11}, isbn = {0141-5492}, pages = {861 -- 866}, year = {2002}, language = {en} } @article{LowisFergusonPaulssenetal.2021, author = {Lowis, Carsten and Ferguson, Simon and Paulßen, Elisabeth and Hoehr, Cornelia}, title = {Improved Sc-44 production in a siphon-style liquid target on a medical cyclotron}, series = {Applied Radiation and Isotopes}, volume = {172}, journal = {Applied Radiation and Isotopes}, number = {Art. 109675}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0969-8043}, doi = {10.1016/j.apradiso.2021.109675}, year = {2021}, language = {en} } @article{ScheerRossKapelyukhetal.2010, author = {Scheer, Nico and Ross, Jillian and Kapelyukh, Yury and Rode, Anja and Wolf, C. Roland}, title = {In vivo responses of the human and murine pregnane X receptor to dexamethasone in mice}, series = {Drug Metabolism and Disposition}, volume = {38}, journal = {Drug Metabolism and Disposition}, number = {7}, publisher = {ASPET}, address = {Bethesda}, issn = {1521-009X}, doi = {10.1124/dmd.109.031872}, pages = {1046 -- 1053}, year = {2010}, abstract = {Dexamethasone (DEX) is a potent and widely used anti-inflammatory and immunosuppressant glucocorticoid. It can bind and activate the pregnane X receptor (PXR), which plays a critical role as xenobiotic sensor in mammals to induce the expression of many enzymes, including cytochromes P450 in the CYP3A family. This induction results in its own metabolism. We have used a series of transgenic mouse lines, including a novel, improved humanized PXR line, to compare the induction profile of PXR-regulated drug-metabolizing enzymes after DEX administration, as well as looking at hepatic responses to rifampicin (RIF). The new humanized PXR model has uncovered further intriguing differences between the human and mouse receptors in that RIF only induced Cyp2b10 in the new humanized model. DEX was found to be a much more potent inducer of Cyp3a proteins in wild-type mice than in mice humanized for PXR. To assess whether PXR is involved in the detoxification of DEX in the liver, we analyzed the consequences of high doses of the glucocorticoid on hepatotoxicity on different PXR genetic backgrounds. We also studied these effects in an additional mouse model in which functional mouse Cyp3a genes have been deleted. These strains exhibited different sensitivities to DEX, indicating a protective role of the PXR and CYP3A proteins against the hepatotoxicity of this compound.}, language = {en} } @article{TeumerCapitainRossJonesetal.2018, author = {Teumer, T. and Capitain, C. and Ross-Jones, J. and Tippk{\"o}tter, Nils and R{\"a}dle, M. and Methner, F.-J.}, title = {In-line Haze Monitoring Using a Spectrally Resolved Back Scattering Sensor}, series = {BrewingScience}, volume = {71}, journal = {BrewingScience}, number = {5/6}, publisher = {Fachverlag Hans Carl}, address = {N{\"u}rnberg}, issn = {1613-2041}, pages = {49 -- 55}, year = {2018}, abstract = {In the present work an optical sensor in combination with a spectrally resolved detection device for in-line particle-size-monitoring for quality control in beer production is presented. The principle relies on the size and wavelength dependent backscatter of growing particles in fluids. Measured interference structures of backscattered light are compared with calculated theoretical values, based on Mie-Theory, and fitted with a linear least square method to obtain particle size distributions. For this purpose, a broadband light source in combination with a process-CCD-spectrometer (charge ? coupled device spectrometer) and process adapted fiber optics are used. The goal is the development of an easy and flexible measurement device for in-line-monitoring of particle size. The presented device can be directly installed in product fill tubes or vessels, follows CIP- (cleaning in place) and removes the need of sample taking. A proof of concept and preliminary results, measuring protein precipitation, are presented.}, language = {en} } @article{EngelHoltmannUlberetal.2018, author = {Engel, Mareike and Holtmann, Dirk and Ulber, Roland and Tippk{\"o}tter, Nils}, title = {Increased Biobutanol Production by Mediator-Less Electro-Fermentation}, series = {Biotechnology Journal}, journal = {Biotechnology Journal}, number = {Volume 14, Issue 4}, publisher = {Wiley-VCH}, issn = {1860-7314}, doi = {10.1002/biot.201800514}, pages = {Artikel 1800514}, year = {2018}, abstract = {A future bio-economy should not only be based on renewable raw materials but also in the raise of carbon yields of existing production routes. Microbial electrochemical technologies are gaining increased attention for this purpose. In this study, the electro-fermentative production of biobutanol with C. acetobutylicum without the use of exogenous mediators is investigated regarding the medium composition and the reactor design. It is shown that the use of an optimized synthetic culture medium allows higher product concentrations, increased biofilm formation, and higher conductivities compared to a synthetic medium supplemented with yeast extract. Moreover, the optimization of the reactor system results in a doubling of the maximum product concentrations for fermentation products. When a working electrode is polarized at -600 mV vs. Ag/AgCl, a shift from butyrate to acetone and butanol production is induced. This leads to an increased final solvent yield of Yᴀᴃᴇ = 0.202 gg⁻¹ (control 0.103 gg⁻¹), which is also reflected in a higher carbon efficiency of 37.6\% compared to 23.3\% (control) as well as a fourfold decrease in simplified E-factor to 0.43. The results are promising for further development of biobutanol production in bioelectrochemical systems in order to fulfil the principles of Green Chemistry.}, language = {en} } @article{MartinezJakobTuetal.2013, author = {Martinez, Ronny and Jakob, Felix and Tu, Ran and Siegert, Petra and Maurer, Karl-Heinz and Schwaneberg, Ulrich}, title = {Increasing activity and thermal resistance of Bacillus gibsonii alkaline protease (BgAP) by directed evolution}, series = {Biotechnology and bioengineering}, volume = {Vol. 110}, journal = {Biotechnology and bioengineering}, number = {Iss. 3}, publisher = {Wiley}, address = {Weinheim}, issn = {1097-0290 (E-Journal); 0006-3592 (Print); 0368-1467 (Print)}, pages = {711 -- 720}, year = {2013}, language = {en} } @article{HeroldBrandtSeibleretal.2008, author = {Herold, Marco J. and Brandt, Jens van den and Seibler, Jost and Reichardt, Holger M.}, title = {Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats}, series = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {105}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, number = {47}, issn = {1091-6490}, doi = {10.1073/pnas.0806213105}, pages = {18507 -- 18512}, year = {2008}, language = {en} } @article{KotnikPopovaTodirasetal.2009, author = {Kotnik, Katarina and Popova, Elena and Todiras, Mihail and Mori, Marcelo A. and Alenina, Natalia and Seibler, Jost and Bader, Michael}, title = {Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown}, series = {Plos One}, volume = {4}, journal = {Plos One}, number = {4}, publisher = {Plos}, address = {San Francisco, California, US}, doi = {10.1371/journal.pone.0005124}, pages = {e5124}, year = {2009}, language = {en} } @article{PaulssenLeLengkeeketal.2013, author = {Paulßen, Elisabeth and Le, Van So and Lengkeek, Nigel and Pellegrini, Paul and Jackson, Tim and Greguric, Ivan and Weiner, Ron}, title = {Influence of Metal Ions on the 68Ga-labeling of DOTATATE}, series = {Applied Radiation and Isotopes}, volume = {82}, journal = {Applied Radiation and Isotopes}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1872-9800}, doi = {10.1016/j.apradiso.2013.08.010}, pages = {232 -- 238}, year = {2013}, language = {en} } @article{ElBerguiAbouabdillahBouriougetal.2023, author = {El Bergui, Omnia and Abouabdillah, Aziz and Bourioug, Mohamed and Schmitz, Dominik and Biel, Markus and Aboudrare, Abdellah and Krauss, Manuel and Jomaa, Ahlem and Romuli, Sebastian and M{\"u}ller, Joachim and Fagroud, Mustapha and Bouabid, Rachid}, title = {Innovative solutions for drought: Evaluating hydrogel application on onion cultivation (Allium cepa) in Morocco}, series = {Water}, volume = {15}, journal = {Water}, number = {11}, publisher = {MDPI}, address = {Basel}, doi = {10.3390/w15111972}, pages = {Artikel 1972}, year = {2023}, abstract = {Throughout the last decade, and particularly in 2022, water scarcity has become a critical concern in Morocco and other Mediterranean countries. The lack of rainfall during spring was worsened by a succession of heat waves during the summer. To address this drought, innovative solutions, including the use of new technologies such as hydrogels, will be essential to transform agriculture. This paper presents the findings of a study that evaluated the impact of hydrogel application on onion (Allium cepa) cultivation in Meknes, Morocco. The treatments investigated in this study comprised two different types of hydrogel-based soil additives (Arbovit® polyacrylate and Huminsorb® polyacrylate), applied at two rates (30 and 20 kg/ha), and irrigated at two levels of water supply (100\% and 50\% of daily crop evapotranspiration; ETc). Two control treatments were included, without hydrogel application and with both water amounts. The experiment was conducted in an open field using a completely randomized design. The results indicated a significant impact of both hydrogel-type dose and water dose on onion plant growth, as evidenced by various vegetation parameters. Among the hydrogels tested, Huminsorb® Polyacrylate produced the most favorable outcomes, with treatment T9 (100\%, HP, 30 kg/ha) yielding 70.55 t/ha; this represented an increase of 11 t/ha as compared to the 100\% ETc treatment without hydrogel application. Moreover, the combination of hydrogel application with 50\% ETc water stress showed promising results, with treatment T4 (HP, 30 kg, 50\%) producing almost the same yield as the 100\% ETc treatment without hydrogel while saving 208 mm of water.}, language = {en} } @article{BiselliThoemmesGarskeetal.1992, author = {Biselli, Manfred and Th{\"o}mmes, J. and Garske, U. and Wandrey, Christian}, title = {Integrated detoxification: Reduction of ammonium concentration by dialysis with cation exchange membranes / Th{\"o}mmes, J. ; Garske, U. ; Biselli, M. ; Wandrey, C.}, series = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, journal = {Animal cell technology : developments, processes, and products ; ESACT, European Society for Animal Cell Technology, the 11th meeting / Ed. R. E. Spier}, publisher = {Butterworth-Heinemann}, address = {Oxford}, isbn = {0750604212}, pages = {171 -- 175}, year = {1992}, language = {en} } @article{SchiedermeierRettnerHeilmannetal.2019, author = {Schiedermeier, Maximilian and Rettner, Cornelius and Heilmann, Marcel and Schneider, Felix and Marz, Martin}, title = {Interference of automotive HV-DC-systems by traction voltage-source-inverters (VSI)}, series = {2019 IEEE Transportation Electrification Conference (ITEC-India)}, journal = {2019 IEEE Transportation Electrification Conference (ITEC-India)}, publisher = {IEEE}, address = {New York}, doi = {10.1109/ITEC-India48457.2019.ITECINDIA2019-37}, pages = {1 -- 6}, year = {2019}, language = {en} } @article{ElbersBehnerRemmeetal.1986, author = {Elbers, Gereon and Behner, T. and Remme, S. and Prissok, F.}, title = {Interstitial Metal Impurities as a Possible Cause of Enhanced Reactivities / T. Behner, G. Elbers, S. Remme, F. Prissok, P. Stegger, and G. Lehmann}, series = {Berichte der Bunsen-Gesellschaft : physical chemistry, chemical physics. 90 (1986)}, journal = {Berichte der Bunsen-Gesellschaft : physical chemistry, chemical physics. 90 (1986)}, isbn = {0005-9021}, pages = {698}, year = {1986}, language = {en} } @article{MangKolzMartinsetal.2006, author = {Mang, Thomas and Kolz, J. and Martins, J. and Kremer, K.}, title = {Investigation of Elastomer Foams by Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Bl{\"u}mich, B. ; Mang, Thomas}, series = {Kurzfassungen : 3. bis 6. Juli 2006, N{\"u}rnberg = Summaries / Deutsche Kautschuk Tagung, 3.7.-6.7.2006}, journal = {Kurzfassungen : 3. bis 6. Juli 2006, N{\"u}rnberg = Summaries / Deutsche Kautschuk Tagung, 3.7.-6.7.2006}, publisher = {DKG, Dt. Kautschuk-Ges.}, address = {Frankfurt, Main}, pages = {33}, year = {2006}, language = {en} } @article{RaueWambachGloeggleretal.2014, author = {Raue, Markus and Wambach, M. and Gl{\"o}ggler, S. and Grefen, Dana and Kaufmann, R. and Abetz, C. and Georgopanos, P. and Handge, U. A. and Mang, Thomas and Bl{\"u}mich, B. and Abetz, V.}, title = {Investigation of historical hard rubber ornaments of Charles Goodyear}, series = {Macromolecular chemistry and physics}, volume = {Vol. 215}, journal = {Macromolecular chemistry and physics}, number = {No. 3}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1022-1352}, pages = {245 -- 254}, year = {2014}, language = {en} } @article{MangKolzHungeretal.2007, author = {Mang, Thomas and Kolz, J. and Hunger, K. and Rath, S.}, title = {Investigation of self-diffusion processes in ionic hydrogels with a single-sided sensor / Kolz, J. ; Hunger, K. ; Rath, S. ; Mang, T. ; Bl{\"u}mich, B.}, series = {Magnetic Resonance Imaging. 25 (2007), H. 4}, journal = {Magnetic Resonance Imaging. 25 (2007), H. 4}, isbn = {0730-725X}, pages = {566 -- 567}, year = {2007}, language = {en} } @article{MangKolzMartinsetal.2007, author = {Mang, Thomas and Kolz, J. and Martins, J. and Kremer, K.}, title = {Investigation of the Elastomer-Foam Production with Single-Sided NMR / Kolz, J. ; Martins, J. ; Kremer, K. ; Mang, Thomas ; Bl{\"u}mich, B.}, series = {Kautschuk Gummi Kunststoffe. 60 (2007), H. 4}, journal = {Kautschuk Gummi Kunststoffe. 60 (2007), H. 4}, isbn = {0948-3276}, pages = {179 -- 183}, year = {2007}, language = {en} } @article{BreuerMangSchoeningetal.2017, author = {Breuer, Lars and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, Ronald and Wagner, Torsten}, title = {Investigation of the spatial resolution of a laser-based stimulation process for light-addressable hydrogels with incorporated graphene oxide by means of IR thermography}, series = {Sensors and Actuators A: Physical}, volume = {268}, journal = {Sensors and Actuators A: Physical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0924-4247}, doi = {10.1016/j.sna.2017.11.031}, pages = {126 -- 132}, year = {2017}, language = {en} } @article{ChristophBahrenbergVryetal.2008, author = {Christoph, Thomas and Bahrenberg, Gregor and Vry, Jean de and Englberger, Werner and Erdmann, Volker A. and Frech, Moritz and K{\"o}gel, Babette and R{\"o}hl, Thomas and Schiene, Klaus and Schr{\"o}der, Wolfgang and Seibler, Jost and Kurreck, Jens}, title = {Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice}, series = {Molecular and Cellular Neuroscience}, volume = {37}, journal = {Molecular and Cellular Neuroscience}, number = {3}, issn = {1044-7431}, doi = {10.1016/j.mcn.2007.12.006}, pages = {579 -- 589}, year = {2008}, language = {en} } @article{FeuerriegelKlose1998, author = {Feuerriegel, Uwe and Klose, W.}, title = {Investigations into the Mechanism of the Deactivation of a Palladium-Supported Catalyst by Hydrogen Sulfide Poisoning. Klose, W. ; Feuerriegel, U.}, series = {Chemical engineering \& technology. 21 (1998), H. 7}, journal = {Chemical engineering \& technology. 21 (1998), H. 7}, isbn = {0930-7516}, pages = {562 -- 564}, year = {1998}, language = {en} }