@article{SchoeningMourzinaSchubertetal.2000, author = {Sch{\"o}ning, Michael Josef and Mourzina, Y. G. and Schubert, J. and Zander, W. and Legin, A. and Vlasov, Y. G. and L{\"u}th, H.}, title = {Can pulsed laser deposition serve as an advanced technique in fabricating chemical sensors?}, series = {Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]}, journal = {Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]}, publisher = {MIC, Mikroelektronik Centret}, address = {Lyngby, Denmark}, isbn = {87-89935-50-0}, pages = {615 -- 618}, year = {2000}, language = {en} } @article{PoghossianAbouzarSchoening2008, author = {Poghossian, Arshak and Abouzar, Maryam H. and Sch{\"o}ning, Michael Josef}, title = {Capacitance-voltage and impedance characteristics of field-effect EIS sensors functionalised with polyelectrolyte multilayers}, series = {IRBM. 29 (2008), H. 2-3}, journal = {IRBM. 29 (2008), H. 2-3}, isbn = {1959-0318}, pages = {149 -- 154}, year = {2008}, language = {en} } @article{AbouzarWernerSchoeningetal.2011, author = {Abouzar, Maryam H. and Werner, Moritz and Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {Capacitance-voltage and impedance-spectroscopy characteristics of nanoplate EISOI capacitors}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley-VCH}, address = {Berlin}, isbn = {1862-6319}, pages = {1327 -- 1332}, year = {2011}, language = {en} } @article{AbouzarPoghossianSiqueiraetal.2010, author = {Abouzar, Maryam H. and Poghossian, Arshak and Siqueira, Jos{\´e} R. Jr. and Oliveira, Osvaldo N. Jr. and Moritz, Werner and Sch{\"o}ning, Michael Josef}, title = {Capacitive electrolyte-insulator-semiconductor structures functionalised with a polyelectrolyte/enzyme multilayer: New strategy for enhanced field-effect biosensing}, series = {Physica Status Solidi (A). 207 (2010), H. 4}, journal = {Physica Status Solidi (A). 207 (2010), H. 4}, isbn = {1862-6300}, pages = {884 -- 890}, year = {2010}, language = {en} } @article{BaeckerPoghossianAbouzaretal.2010, author = {B{\"a}cker, Matthias and Poghossian, Arshak and Abouzar, Maryam H. and Wenmackers, Sylvia and Janssens, Stoffel D. and Haenen, Ken and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Capacitive field-effect (bio-)chemical sensors based on nanocrystalline diamond films}, series = {Diamond Electronics and Bioelectronics — Fundamentals to Applications III, edited by P. Bergonzo, [u.a.]}, journal = {Diamond Electronics and Bioelectronics — Fundamentals to Applications III, edited by P. Bergonzo, [u.a.]}, pages = {1 -- 6}, year = {2010}, language = {en} } @article{JablonskiPoghossianSeverinetal.2021, author = {Jablonski, Melanie and Poghossian, Arshak and Severin, Robin and Keusgen, Michael and Wege, Christian and Sch{\"o}ning, Michael Josef}, title = {Capacitive Field-Effect Biosensor Studying Adsorption of Tobacco Mosaic Virus Particles}, series = {Micromachines}, volume = {12}, journal = {Micromachines}, number = {1}, publisher = {MDPI}, address = {Basel}, doi = {10.3390/mi12010057}, pages = {Artikel 57}, year = {2021}, abstract = {Plant virus-like particles, and in particular, tobacco mosaic virus (TMV) particles, are increasingly being used in nano- and biotechnology as well as for biochemical sensing purposes as nanoscaffolds for the high-density immobilization of receptor molecules. The sensitive parameters of TMV-assisted biosensors depend, among others, on the density of adsorbed TMV particles on the sensor surface, which is affected by both the adsorption conditions and surface properties of the sensor. In this work, Ta₂O₅-gate field-effect capacitive sensors have been applied for the label-free electrical detection of TMV adsorption. The impact of the TMV concentration on both the sensor signal and the density of TMV particles adsorbed onto the Ta₂O₅-gate surface has been studied systematically by means of field-effect and scanning electron microscopy methods. In addition, the surface density of TMV particles loaded under different incubation times has been investigated. Finally, the field-effect sensor also demonstrates the label-free detection of penicillinase immobilization as model bioreceptor on TMV particles.}, language = {en} } @article{PoghossianSchoening2020, author = {Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Capacitive field-effect eis chemical sensors and biosensors: A status report}, series = {Sensors}, volume = {20}, journal = {Sensors}, number = {19}, publisher = {MDPI}, address = {Basel}, issn = {1424-8220}, doi = {10.3390/s20195639}, pages = {Artikel 5639}, year = {2020}, abstract = {Electrolyte-insulator-semiconductor (EIS) field-effect sensors belong to a new generation of electronic chips for biochemical sensing, enabling a direct electronic readout. The review gives an overview on recent advances and current trends in the research and development of chemical sensors and biosensors based on the capacitive field-effect EIS structure—the simplest field-effect device, which represents a biochemically sensitive capacitor. Fundamental concepts, physicochemical phenomena underlying the transduction mechanism and application of capacitive EIS sensors for the detection of pH, ion concentrations, and enzymatic reactions, as well as the label-free detection of charged molecules (nucleic acids, proteins, and polyelectrolytes) and nanoparticles, are presented and discussed.}, language = {en} } @article{BuniatyanMatirosyanAbouzaretal.2009, author = {Buniatyan, Vahe V. and Matirosyan, N. and Abouzar, Maryam H. and Schubert, J. and Zander, W. and Gevorgian, S. and Sch{\"o}ning, Michael Josef and Poghossian, Arshak}, title = {Capacitive field-effect pH sensor based on an electrolyte-ferroelectric-insulator-semiconductor structure}, series = {SENSOR 2009 : : 14th International Conference on Sensors, Technologies, Electronics and Applications; N{\"u}rnberg, Germany, 26 - 28 May 2009; proceedings; [part of] Sensor + Test Conference 2009 / AMA, Fachverband f{\"u}r Sensorik e.V}, journal = {SENSOR 2009 : : 14th International Conference on Sensors, Technologies, Electronics and Applications; N{\"u}rnberg, Germany, 26 - 28 May 2009; proceedings; [part of] Sensor + Test Conference 2009 / AMA, Fachverband f{\"u}r Sensorik e.V}, publisher = {AMA Service}, address = {Wunstorf}, isbn = {9783981099355}, pages = {317 -- 322}, year = {2009}, language = {en} } @article{JablonskiMuenstermannNorketal.2021, author = {Jablonski, Melanie and M{\"u}nstermann, Felix and Nork, Jasmina and Molinnus, Denise and Muschallik, Lukas and Bongaerts, Johannes and Wagner, Torsten and Keusgen, Michael and Siegert, Petra and Sch{\"o}ning, Michael Josef}, title = {Capacitive field-effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths}, series = {physica status solidi (a) applications and materials science}, volume = {218}, journal = {physica status solidi (a) applications and materials science}, number = {13}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.202000765}, pages = {7 Seiten}, year = {2021}, abstract = {An acetoin biosensor based on a capacitive electrolyte-insulator-semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance-voltage, and constant capacitance methods, respectively.}, language = {en} } @article{SchoeningKurowskiThustetal.2000, author = {Sch{\"o}ning, Michael Josef and Kurowski, A. and Thust, M. and Kordos, P. and Schultze, J. W. and L{\"u}th, H.}, title = {Capacitive microsensors for biochemical sensing on porous silicon technology}, series = {Sensors and Actuators B. 64 (2000), H. 1-3}, journal = {Sensors and Actuators B. 64 (2000), H. 1-3}, isbn = {0925-4005}, pages = {59 -- 64}, year = {2000}, language = {en} } @article{KarschuckPoghossianSeretal.2024, author = {Karschuck, Tobias and Poghossian, Arshak and Ser, Joey and Tsokolakyan, Astghik and Achtsnicht, Stefan and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Capacitive model of enzyme-modified field-effect biosensors: Impact of enzyme coverage}, series = {Sensors and Actuators B: Chemical}, volume = {408}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005 (Print)}, doi = {10.1016/j.snb.2024.135530}, pages = {12 Seiten}, year = {2024}, abstract = {Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.}, language = {en} } @article{HuckPoghossianBaeckeretal.2014, author = {Huck, Christina and Poghossian, Arshak and B{\"a}cker, Matthias and Chaudhuri, S. and Zander, W. and Schubert, J. and Begoyan, V. K. and Buniatyan, V. V. and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Capacitively coupled electrolyte-conductivity sensor based on high-k material of barium strontium titanate}, series = {Sensors and actuators. B: Chemical}, journal = {Sensors and actuators. B: Chemical}, number = {198}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-3077 (E-Journal); 0925-4005 (Print)}, doi = {10.1016/j.snb.2014.02.103}, pages = {102 -- 109}, year = {2014}, language = {en} } @article{RodriguesMoraisNordietal.2018, author = {Rodrigues, Raul T. and Morais, Paulo V. and Nordi, Cristina S. F. and Sch{\"o}ning, Michael Josef and Siqueira Jr., Jos{\´e} R. and Caseli, Luciano}, title = {Carbon Nanotubes and Algal Polysaccharides To Enhance the Enzymatic Properties of Urease in Lipid Langmuir-Blodgett Films}, series = {Langmuir}, volume = {34}, journal = {Langmuir}, number = {9}, publisher = {ACS Publications}, address = {Washington, DC}, issn = {1520-5827}, doi = {10.1021/acs.langmuir.7b04317}, pages = {3082 -- 3093}, year = {2018}, abstract = {Algal polysaccharides (extracellular polysaccharides) and carbon nanotubes (CNTs) were adsorbed on dioctadecyldimethylammonium bromide Langmuir monolayers to serve as a matrix for the incorporation of urease. The physicochemical properties of the supramolecular system as a monolayer at the air-water interface were investigated by surface pressure-area isotherms, surface potential-area isotherms, interfacial shear rheology, vibrational spectroscopy, and Brewster angle microscopy. The floating monolayers were transferred to hydrophilic solid supports, quartz, mica, or capacitive electrolyte-insulator-semiconductor (EIS) devices, through the Langmuir-Blodgett (LB) technique, forming mixed films, which were investigated by quartz crystal microbalance, fluorescence spectroscopy, and field emission gun scanning electron microscopy. The enzyme activity was studied with UV-vis spectroscopy, and the feasibility of the thin film as a urea sensor was essayed in an EIS sensor device. The presence of CNT in the enzyme-lipid LB film not only tuned the catalytic activity of urease but also helped to conserve its enzyme activity. Viability as a urease sensor was demonstrated with capacitance-voltage and constant capacitance measurements, exhibiting regular and distinctive output signals over all concentrations used in this work. These results are related to the synergism between the compounds on the active layer, leading to a surface morphology that allowed fast analyte diffusion owing to an adequate molecular accommodation, which also preserved the urease activity. This work demonstrates the feasibility of employing LB films composed of lipids, CNT, algal polysaccharides, and enzymes as EIS devices for biosensing applications.}, language = {en} } @article{SchollMoraisGabrieletal.2017, author = {Scholl, Fabio and Morais, Paulo and Gabriel, Rayla and Sch{\"o}ning, Michael Josef and Siqueira, Jose Roberto, Jr. and Caseli, Luciano}, title = {Carbon nanotubes arranged as smart interfaces in lipid Langmuir-Blodgett films enhancing the enzymatic properties of penicillinase for biosensing applications}, series = {Applied Materials \& Interfaces}, volume = {9}, journal = {Applied Materials \& Interfaces}, number = {36}, publisher = {ACS}, address = {Washington}, issn = {1944-8252}, doi = {10.1021/acsami.7b08095}, pages = {31054 -- 31066}, year = {2017}, abstract = {In this paper, carbon nanotubes (CNTs) were incorporated in penicillinase-phospholipid Langmuir and Langmuir-Blodgett (LB) films to enhance the enzyme catalytic properties. Adsorption of the penicillinase and CNTs at dimyristoylphosphatidic acid (DMPA) monolayers at the air-water interface was investigated by surface pressure-area isotherms, vibrational spectroscopy, and Brewster angle microscopy. The floating monolayers were transferred to solid supports through the LB technique, forming mixed DMPA-CNTs-PEN films, which were investigated by quartz crystal microbalance, vibrational spectroscopy, and atomic force microscopy. Enzyme activity was studied with UV-vis spectroscopy and the feasibility of the supramolecular device nanostructured as ultrathin films were essayed in a capacitive electrolyte-insulator-semiconductor (EIS) sensor device. The presence of CNTs in the enzyme-lipid LB film not only tuned the catalytic activity of penicillinase but also helped conserve its enzyme activity after weeks, showing increased values of activity. Viability as penicillin sensor was demonstrated with capacitance/voltage and constant capacitance measurements, exhibiting regular and distinctive output signals over all concentrations used in this work. These results may be related not only to the nanostructured system provided by the film, but also to the synergism between the compounds on the active layer, leading to a surface morphology that allowed a fast analyte diffusion because of an adequate molecular accommodation, which also preserved the penicillinase activity. This work therefore demonstrates the feasibility of employing LB films composed of lipids, CNTs, and enzymes as EIS devices for biosensing applications.}, language = {en} } @article{SiqueiraAbouzarBaeckeretal.2009, author = {Siqueira, Jos{\´e} R. Jr. and Abouzar, Maryam H. and B{\"a}cker, Matthias and Zucolotto, Valtencir and Poghossian, Arshak and Oliveira, Osvaldo N. Jr. and Sch{\"o}ning, Michael Josef}, title = {Carbon nanotubes in nanostructured films: Potential application as amperometric and potentiometric field-effect (bio-)chemical sensors}, series = {physica status solidi (a) . 206 (2009), H. 3}, journal = {physica status solidi (a) . 206 (2009), H. 3}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {462 -- 467}, year = {2009}, language = {en} } @article{HandtkeVollandMethlingetal.2014, author = {Handtke, Stefan and Volland, Sonja and Methling, Karen and Albrecht, Dirk and Becher, D{\"o}rte and Nehls, Jenny and Bongaerts, Johannes and Maurer, Karl-Heinz and Lalk, Michael and Liesegang, Heiko and Voigt, Birgit and Daniel, Rolf and Hecker, Michael}, title = {Cell physiology of the biotechnological relevant bacterium Bacillus pumilus - An omics-based approach}, series = {Journal of Biotechnology}, journal = {Journal of Biotechnology}, number = {192(A)}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2014.08.028}, pages = {204 -- 214}, year = {2014}, abstract = {Members of the species Bacillus pumilus get more and more in focus of the biotechnological industry as potential new production strains. Based on exoproteome analysis, B. pumilus strain Jo2, possessing a high secretion capability, was chosen for an omics-based investigation. The proteome and metabolome of B. pumilus cells growing either in minimal or complex medium was analyzed. In total, 1542 proteins were identified in growing B. pumilus cells, among them 1182 cytosolic proteins, 297 membrane and lipoproteins and 63 secreted proteins. This accounts for about 43\% of the 3616 proteins encoded in the B. pumilus Jo2 genome sequence. By using GC-MS, IP-LC/MS and H NMR methods numerous metabolites were analyzed and assigned to reconstructed metabolic pathways. In the genome sequence a functional secretion system including the components of the Sec- and Tat-secretion machinery was found. Analysis of the exoproteome revealed secretion of about 70 proteins with predicted secretion signals. In addition, selected production-relevant genome features such as restriction modification systems and NRPS clusters of B. pumilus Jo2 are discussed.}, language = {en} } @article{HaegerWirgesTanzmannetal.2023, author = {Haeger, Gerrit and Wirges, Jessika and Tanzmann, Nicole and Oyen, Sven and Jolmes, Tristan and Jaeger, Karl-Erich and Sch{\"o}rken, Ulrich and Bongaerts, Johannes and Siegert, Petra}, title = {Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis}, series = {Microbial Cell Factories}, journal = {Microbial Cell Factories}, number = {22}, publisher = {Springer Nature}, issn = {1475-2859}, doi = {10.1186/s12934-023-02079-1}, pages = {Article number: 77 (2023)}, year = {2023}, abstract = {Background Aminoacylases are highly promising enzymes for the green synthesis of acyl-amino acids, potentially replacing the environmentally harmful Schotten-Baumann reaction. Long-chain acyl-amino acids can serve as strong surfactants and emulsifiers, with application in cosmetic industries. Heterologous expression of these enzymes, however, is often hampered, limiting their use in industrial processes. Results We identified a novel mycobacterial aminoacylase gene from Mycolicibacterium smegmatis MKD 8, cloned and expressed it in Escherichia coli and Vibrio natriegens using the T7 overexpression system. The recombinant enzyme was prone to aggregate as inclusion bodies, and while V. natriegens Vmax™ could produce soluble aminoacylase upon induction with isopropyl β-d-1-thiogalactopyranoside (IPTG), E. coli BL21 (DE3) needed autoinduction with lactose to produce soluble recombinant protein. We successfully conducted a chaperone co-expression study in both organisms to further enhance aminoacylase production and found that overexpression of chaperones GroEL/S enhanced aminoacylase activity in the cell-free extract 1.8-fold in V. natriegens and E. coli. Eventually, E. coli ArcticExpress™ (DE3), which co-expresses cold-adapted chaperonins Cpn60/10 from Oleispira antarctica, cultivated at 12 °C, rendered the most suitable expression system for this aminoacylase and exhibited twice the aminoacylase activity in the cell-free extract compared to E. coli BL21 (DE3) with GroEL/S co-expression at 20 °C. The purified aminoacylase was characterized based on hydrolytic activities, being most stable and active at pH 7.0, with a maximum activity at 70 °C, and stability at 40 °C and pH 7.0 for 5 days. The aminoacylase strongly prefers short-chain acyl-amino acids with smaller, hydrophobic amino acid residues. Several long-chain amino acids were fairly accepted in hydrolysis as well, especially N-lauroyl-L-methionine. To initially evaluate the relevance of this aminoacylase for the synthesis of N-acyl-amino acids, we demonstrated that lauroyl-methionine can be synthesized from lauric acid and methionine in an aqueous system. Conclusion Our results suggest that the recombinant enzyme is well suited for synthesis reactions and will thus be further investigated.}, language = {en} } @article{ReisertGeisslerFloerkeetal.2013, author = {Reisert, Steffen and Geissler, Hanno and Fl{\"o}rke, Rudolf and Weiler, Christian and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Characterisation of aseptic sterilisation processes using an electronic nose}, series = {International journal of nanotechnology}, volume = {Vol. 10}, journal = {International journal of nanotechnology}, number = {No. 5-7}, publisher = {Inderscience Enterprises}, address = {Gen{\`e}ve}, issn = {1475-7435 (Print) 7141-8151 (Online)}, pages = {470 -- 484}, year = {2013}, language = {en} } @article{AbouzarPoghossianRazavietal.2009, author = {Abouzar, Maryam H. and Poghossian, Arshak and Razavi, A. and Williams, O. A. and Bijnens, N. and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Characterisation of capacitive field-effect sensors with a nanocrystalline-diamond film as transducer material for multi-parameter sensing}, series = {Biosensors and Bioelectronics. 24 (2009), H. 5}, journal = {Biosensors and Bioelectronics. 24 (2009), H. 5}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0956-5663}, pages = {1298 -- 1304}, year = {2009}, language = {en} } @article{KirchnerReisertPuetzetal.2012, author = {Kirchner, Patrick and Reisert, Steffen and P{\"u}tz, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Characterisation of polymeric materials as passivation layer for calorimetric H2O2 gas sensors}, series = {Physica Status Solidi (a)}, volume = {209}, journal = {Physica Status Solidi (a)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201100773}, pages = {859 -- 863}, year = {2012}, abstract = {Calorimetric gas sensors for monitoring the H₂O₂ concentration at elevated temperatures in industrial sterilisation processes have been presented in previous works. These sensors are built up in form of a differential set-up of a catalytically active and passive temperature-sensitive structure. Although, various types of catalytically active dispersions have been studied, the passivation layer has to be established and therefore, chemically as well as physically characterised. In the present work, fluorinated ethylene propylene (FEP), perfluoralkoxy (PFA) and epoxy-based SU-8 photoresist as temperature-stable polymeric materials have been investigated for sensor passivation in terms of their chemical inertness against H₂O₂, their hygroscopic properties as well as their morphology. The polymeric materials were deposited via spin-coating on the temperature-sensitive structure, wherein spin-coated FEP and PFA show slight agglomerates. However, they possess a low absorption of humidity due to their hydrophobic surface, whereas the SU-8 layer has a closed surface but shows a slightly higher absorption of water. All of them were inert against gaseous H₂O₂ during the characterisation in H₂O₂ atmosphere that demonstrates their suitability as passivation layer for calorimetric H₂O₂ gas sensors.}, language = {en} } @article{SchrothLuethHummeletal.2001, author = {Schroth, P. and L{\"u}th, H. and Hummel, Hans E. and Sch{\"u}tz, S. and Sch{\"o}ning, Michael Josef}, title = {Characterising an insect antenna as a receptor for a biosensor by means of impedance spectroscopy}, series = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, journal = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, publisher = {Elsevier [u.a.]}, address = {Amsterdam [u.a.]}, isbn = {0-08-044014-2}, pages = {293 -- 297}, year = {2001}, language = {en} } @article{SchusserPoghossianBaeckeretal.2012, author = {Schusser, Sebastian and Poghossian, Arshak and B{\"a}cker, Matthias and Leinhos, Marcel and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Characterization of biodegradable polymers with capacitive field-effect sensors}, series = {Sensors and actuators B: Chemical}, volume = {187}, journal = {Sensors and actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2012.07.099}, pages = {2 -- 7}, year = {2012}, abstract = {In vitro studies of the degradation kinetic of biopolymers are essential for the design and optimization of implantable biomedical devices. In the presented work, a field-effect capacitive sensor has been applied for the real-time and in situ monitoring of degradation processes of biopolymers for the first time. The polymer-covered field-effect sensor is, in principle, capable to detect any changes in bulk, surface and interface properties of the polymer induced by degradation processes. The feasibility of this approach has been experimentally proven by using the commercially available biomedical polymer poly(D,L-lactic acid) (PDLLA) as a model system. PDLLA films of different thicknesses were deposited on the Ta₂O₅-gate surface of the field-effect structure from a polymer solution by means of spin-coating method. The polymer-modified field-effect sensors have been characterized by means of capacitance-voltage and impedance-spectroscopy method. The degradation of the PDLLA was accelerated by changing the degradation medium from neutral (pH 7.2) to alkaline (pH 9) condition, resulting in drastic changes in the capacitance and impedance spectra of the polymer-modified field-effect sensor.}, language = {en} } @article{PoghossianSchoening2007, author = {Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Chemical and biological field-effect sensors for liquids - a status report}, series = {Handbook of biosensors and biochips / ed. Robert S. Marks ... Bd. 1}, journal = {Handbook of biosensors and biochips / ed. Robert S. Marks ... Bd. 1}, publisher = {Wiley}, address = {Chichester}, isbn = {978-0-470-01905-4}, pages = {395 -- 412}, year = {2007}, language = {en} } @article{MiyamotoKuwabaraKanohetal.2009, author = {Miyamoto, K. and Kuwabara, Yohei and Kanoh, Shin'ichiro and Yoshinobu, Tatsuo and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Chemical image scanner based on FDM-LAPS}, series = {Sensors and Actuators B: Chemical. 137 (2009), H. 2}, journal = {Sensors and Actuators B: Chemical. 137 (2009), H. 2}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0925-4005}, pages = {533 -- 538}, year = {2009}, language = {en} } @article{MiyamotoIchimuraWagneretal.2012, author = {Miyamoto, K. and Ichimura, H. and Wagner, Torsten and Yoshinobu, T. and Sch{\"o}ning, Michael Josef}, title = {Chemical Imaging of ion Diffusion in a Microfluidic Channel}, series = {Procedia Engineering}, journal = {Procedia Engineering}, number = {47}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2012.09.289}, pages = {886 -- 889}, year = {2012}, abstract = {The chemical imaging sensor is a chemical sensor which is capable of visualizing the spatial distribution of chemical species in sample solution. In this study, a novel measurement system based on the chemical imaging sensor was developed to observe the inside of a Y-shaped microfluidic channel while injecting two sample solutions from two branches. From the collected chemical images, it was clearly observed that the injected solutions formed laminar flows in the microfluidic channel. In addition, ion diffusion across the laminar flows was observed. This label-free method can acquire quantitative data of ion distribution and diffusion in microfluidic devices, which can be used to determine the diffusion coefficients, and therefore, the molecular weights of chemical species in the sample solution.}, language = {en} } @article{MiyamotoIchimuraWagneretal.2013, author = {Miyamoto, Ko-ichiro and Ichimura, Hiroki and Wagner, Torsten and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Chemical imaging of the concentration profile of ion diffusion in a microfluidic channel}, series = {Sensors and actuators. B: Chemical}, volume = {189}, journal = {Sensors and actuators. B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-3077 (E-Journal); 0925-4005 (Print)}, doi = {10.1016/j.snb.2013.04.057}, pages = {240 -- 245}, year = {2013}, abstract = {The chemical imaging sensor is a device to visualize the spatial distribution of chemical species based on the principle of LAPS (light-addressable potentiometric sensor), which is a field-effect chemical sensor based on semiconductor. In this study, the chemical imaging sensor has been applied to investigate the ion profile of laminar flows in a microfluidic channel. The chemical images (pH maps) were collected in a Y-shaped microfluidic channel while injecting HCl and NaCl solutions into two branches. From the chemical images, it was clearly observed that the injected solutions formed laminar flows in the channel. In addition, ion diffusion across the laminar flows was observed, and the diffusion coefficient could be derived by fitting the pH profiles to the Fick's equation.}, language = {en} } @article{YoshinobuEckenIsmailetal.2001, author = {Yoshinobu, T. and Ecken, H. and Ismail, Md.A.B. and Iwasaki, H. and L{\"u}th, H. and Sch{\"o}ning, Michael Josef}, title = {Chemical imaging sensor and its application to biological systems}, series = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, journal = {Scaling down in electrochemistry : electrochemical micro- and nanosystem technology ; proceedings of the 3rd International Symposium on Electrochemical Microsystem Technologies, Garmisch-Patenkirchen, Germany, 11 - 15 September 2000 / ed. by J. W. Schultz}, publisher = {Elsevier [u.a.]}, address = {Amsterdam [u.a.]}, isbn = {0-08-044014-2}, pages = {259 -- 263}, year = {2001}, language = {en} } @article{PoghossianBerndsenSchoening2002, author = {Poghossian, Arshak and Berndsen, L. and Sch{\"o}ning, Michael Josef}, title = {Chemical sensor as a physical sensor: ISFET-based flowvelocity, flow-direction and diffusion-coefficient sensor}, series = {Book of abstracts / ed. by J. Saneistr.}, journal = {Book of abstracts / ed. by J. Saneistr.}, publisher = {Czech Technical University, Faculty of Electrical Engineering, Department of Measurement}, address = {Prague}, isbn = {80-01-02576-4}, pages = {649 -- 652}, year = {2002}, language = {en} } @article{PoghossianBerndsenSchoening2003, author = {Poghossian, Arshak and Berndsen, Lars and Sch{\"o}ning, Michael Josef}, title = {Chemical sensor as physical sensor: ISFET-based flowvelocity, flow-direction and diffusion-coefficient sensor}, series = {Sensors and Actuators B. 95 (2003), H. 1-3}, journal = {Sensors and Actuators B. 95 (2003), H. 1-3}, isbn = {0925-4005}, pages = {384 -- 390}, year = {2003}, language = {en} } @article{BaeckerRakowskiPoghossianetal.2013, author = {B{\"a}cker, Matthias and Rakowski, D. and Poghossian, Arshak and Biselli, Manfred and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Chip-based amperometric enzyme sensor system for monitoring of bioprocesses by flow-injection analysis}, series = {Journal of Biotechnology}, volume = {163}, journal = {Journal of Biotechnology}, number = {4}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0168-1656}, doi = {10.1016/j.jbiotec.2012.03.014}, pages = {371 -- 376}, year = {2013}, abstract = {A microfluidic chip integrating amperometric enzyme sensors for the detection of glucose, glutamate and glutamine in cell-culture fermentation processes has been developed. The enzymes glucose oxidase, glutamate oxidase and glutaminase were immobilized by means of cross-linking with glutaraldehyde on platinum thin-film electrodes integrated within a microfluidic channel. The biosensor chip was coupled to a flow-injection analysis system for electrochemical characterization of the sensors. The sensors have been characterized in terms of sensitivity, linear working range and detection limit. The sensitivity evaluated from the respective peak areas was 1.47, 3.68 and 0.28 μAs/mM for the glucose, glutamate and glutamine sensor, respectively. The calibration curves were linear up to a concentration of 20 mM glucose and glutamine and up to 10 mM for glutamate. The lower detection limit amounted to be 0.05 mM for the glucose and glutamate sensor, respectively, and 0.1 mM for the glutamine sensor. Experiments in cell-culture medium have demonstrated a good correlation between the glutamate, glutamine and glucose concentrations measured with the chip-based biosensors in a differential-mode and the commercially available instrumentation. The obtained results demonstrate the feasibility of the realized microfluidic biosensor chip for monitoring of bioprocesses.}, language = {en} } @article{MolinnusHardtKaeveretal.2018, author = {Molinnus, Denise and Hardt, G. and K{\"a}ver, L. and Willenberg, H.S. and Kr{\"o}ger, J.-C. and Poghossian, Arshak and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Chip-based biosensor for the detection of low adrenaline concentrations to support adrenal venous sampling}, series = {Sensor and Actuators B: Chemical}, volume = {272}, journal = {Sensor and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2018.05.136}, pages = {21 -- 27}, year = {2018}, abstract = {A chip-based amperometric biosensor referring on using the bioelectrocatalytical amplification principle for the detection of low adrenaline concentrations is presented. The adrenaline biosensor has been prepared by modification of a platinum thin-film electrode with an enzyme membrane containing the pyrroloquinoline quinone-dependent glucose dehydrogenase and glutaraldehyde. Measuring conditions such as temperature, pH value, and glucose concentration have been optimized to achieve a high sensitivity and a low detection limit of about 1 nM adrenaline measured in phosphate buffer at neutral pH value. The response of the biosensor to different catecholamines has also been proven. Long-term stability of the adrenaline biosensor has been studied over 10 days. In addition, the biosensor has been successfully applied for adrenaline detection in human blood plasma for future biomedical applications. Furthermore, preliminary experiments have been carried to detect the adrenaline-concentration difference measured in peripheral blood and adrenal venous blood, representing the adrenal vein sampling procedure of a physician.}, language = {en} } @article{SchoeningBrinkmannRolkaetal.2005, author = {Sch{\"o}ning, Michael Josef and Brinkmann, D. and Rolka, David and Demuth, C. and Poghossian, Arshak}, title = {CIP (cleaning-in-place) suitable "non-glass" pH sensor based on a Ta2O5-gate EIS structure}, series = {Sensors and Actuators B: Chemical. 111-112 (2005)}, journal = {Sensors and Actuators B: Chemical. 111-112 (2005)}, isbn = {0925-4005}, pages = {423 -- 429}, year = {2005}, language = {en} } @article{SchoeningBrinkmannDemuthetal.2004, author = {Sch{\"o}ning, Michael Josef and Brinkmann, D. and Demuth, C. and Poghossian, Arshak}, title = {CIP (cleaning-in-place)-suitable „non-glass" pH sensor based on a Ta2O5-gate EIS structure}, series = {Digest of technical papers : September 12 - 15, 2004, Rome, Italy, Pontificia Universitas Sancto Thoma Aquinate in Urbe / [conference chairperson: C. Di Natale].}, journal = {Digest of technical papers : September 12 - 15, 2004, Rome, Italy, Pontificia Universitas Sancto Thoma Aquinate in Urbe / [conference chairperson: C. Di Natale].}, address = {Roma}, isbn = {88-7621-282-5}, pages = {857 -- 860}, year = {2004}, language = {en} } @article{SchiffelsSelmer2019, author = {Schiffels, Johannes and Selmer, Thorsten}, title = {Combinatorial assembly of ferredoxin-linked modules in Escherichia coli yields a testing platform for Rnf-complexes}, series = {Biotechnology and Bioengineering}, journal = {Biotechnology and Bioengineering}, number = {accepted article}, publisher = {Wiley}, address = {Weinheim}, doi = {10.1002/bit.27079}, pages = {1 -- 36}, year = {2019}, language = {en} } @article{HuckPoghossianWagneretal.2012, author = {Huck, Christina and Poghossian, Arshak and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Combined amperometric/field-effect sensor for the detection of dissolved hydrogen}, series = {Sensors and actuators B: Chemical}, volume = {187}, journal = {Sensors and actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2012.10.050}, pages = {168 -- 173}, year = {2012}, abstract = {Real-time and reliable monitoring of the biogas process is crucial for a stable and efficient operation of biogas production in order to avoid digester breakdowns. The concentration of dissolved hydrogen (H₂) represents one of the key parameters for biogas process control. In this work, a one-chip integrated combined amperometric/field-effect sensor for monitoring the dissolved H₂ concentration has been developed for biogas applications. The combination of two different transducer principles might allow a more accurate and reliable measurement of dissolved H₂ as an early warning indicator of digester failures. The feasibility of the approach has been demonstrated by simultaneous amperometric/field-effect measurements of dissolved H₂ concentrations in electrolyte solutions. Both, the amperometric and the field-effect transducer show a linear response behaviour in the H₂ concentration range from 0.1 to 3\% (v/v) with a slope of 198.4 ± 13.7 nA/\% (v/v) and 14.9 ± 0.5 mV/\% (v/v), respectively.}, language = {en} } @article{ArreolaKeusgenWagneretal.2019, author = {Arreola, Julio and Keusgen, Michael and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Combined calorimetric gas- and spore-based biosensor array for online monitoring and sterility assurance of gaseous hydrogen peroxide in aseptic filling machines}, series = {Biosensors and Bioelectronics}, volume = {143}, journal = {Biosensors and Bioelectronics}, number = {111628}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0956-5663}, doi = {10.1016/j.bios.2019.111628}, year = {2019}, language = {en} } @article{WernerTakenagaTakietal.2013, author = {Werner, Frederik and Takenaga, Shoko and Taki, Hidenori and Sawada, Kazuaki and Sch{\"o}ning, Michael Josef}, title = {Comparison of label-free ACh-imaging sensors based on CCD and LAPS}, series = {Sensors and Actuators B: Chemical (2012)}, volume = {177}, journal = {Sensors and Actuators B: Chemical (2012)}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0925-4005}, pages = {745 -- 752}, year = {2013}, abstract = {Semiconductor-based chemical imaging sensors, like the light-addressable potentiometric sensor (LAPS) or the pH-imaging sensor based on a charge-coupled device (CCD), are becoming a powerful tool for label-free imaging of biological phenomena. We have proposed a polyion-based enzymatic membrane to develop an acetylcholine (ACh) imaging sensor for neural cell-activity observations. In this study, a CCD-type ACh-imaging sensor and a LAPS-type ACh-imaging sensor were fabricated and the prospect of both sensors was clarified by making a comparison of their basic characteristics.}, language = {en} } @article{MolinnusBaeckerIkenetal.2015, author = {Molinnus, Denise and B{\"a}cker, Matthias and Iken, Heiko and Poghossian, Arshak and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Concept for a biomolecular logic chip with an integrated sensor and actuator function}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201431913}, pages = {1382 -- 1388}, year = {2015}, abstract = {A concept for a new generation of an integrated multi-functional biosensor/actuator system is developed, which is based on biomolecular logic principles. Such a system is expected to be able to detect multiple biochemical input signals simultaneously and in real-time and convert them into electrical output signals with logical operations such as OR, AND, etc. The system can be designed as a closed-loop drug release device triggered by an enzyme logic gate, while the release of the drug induced by the actuator at the required dosage and timing will be controlled by an additional drug sensor. Thus, the system could help to make an accurate and specific diagnosis. The presented concept is exemplarily demonstrated by using an enzyme logic gate based on a glucose/glucose oxidase system, a temperature-responsive hydrogel mimicking the actuator function and an insulin (drug) sensor. In this work, the results of functional testing of individual amperometric glucose and insulin sensors as well as an impedimetric sensor for the detection of the hydrogel swelling/shrinking are presented.}, language = {en} } @article{BaeckerBegingBisellietal.2009, author = {B{\"a}cker, Matthias and Beging, Stefan and Biselli, Manfred and Poghossian, Arshak and Wang, J. and Zang, Werner and Wagner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Concept for a solid-state multi-parameter sensor system for cell-culture monitoring}, series = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, journal = {Electrochimica Acta. 54 (2009), H. 25 Sp. Iss. SI}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {0013-4686}, pages = {6107 -- 6112}, year = {2009}, language = {en} } @article{SchoeningSchrothSchuetzetal.2000, author = {Sch{\"o}ning, Michael Josef and Schroth, P. and Sch{\"u}tz, S. and Hummel, Hans E. and L{\"u}th, H.}, title = {Connecting an insect antenna to a microchip as a novel type of biosensor}, series = {Proceedings : applications, trends, visions / VDE World Microtechnologies Congress, September 25 - 27, 2000, Expo 2000, Hannover, Germany. Organized by VDE Association for Electrical, Electronic \& Information Technologies ....Vol 2.}, journal = {Proceedings : applications, trends, visions / VDE World Microtechnologies Congress, September 25 - 27, 2000, Expo 2000, Hannover, Germany. Organized by VDE Association for Electrical, Electronic \& Information Technologies ....Vol 2.}, publisher = {VDE-Verl.}, address = {[Berlin] [u.a.]}, isbn = {3-8007-2579-7}, pages = {411 -- 416}, year = {2000}, language = {en} } @article{YoshinobuEckenPoghossianetal.2001, author = {Yoshinobu, T. and Ecken, H. and Poghossian, Arshak and Simonis, A. and Iwasaki, H. and L{\"u}th, H. and Sch{\"o}ning, Michael Josef}, title = {Constant-current-mode LAPS (CLAPS) for the detection of penicillin}, series = {Electroanalysis. 13 (2001), H. 8-9}, journal = {Electroanalysis. 13 (2001), H. 8-9}, isbn = {1040-0397}, pages = {733 -- 736}, year = {2001}, language = {en} } @article{MiyamotoWagnerMimuraetal.2009, author = {Miyamoto, Ko-ichiro and Wagner, Torsten and Mimura, Shuhei and Kanoh, Shin`ichiro and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {Constant-phase-mode operation of the light-addressable potentiometric sensor}, series = {Procedia Chemistry. 1 (2009), H. 1}, journal = {Procedia Chemistry. 1 (2009), H. 1}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1876-6196}, pages = {1487 -- 1490}, year = {2009}, language = {en} } @article{MiyamotoWagnerMimuraetal.2011, author = {Miyamoto, Ko-ichiro and Wagner, Torsten and Mimura, Shuhei and Kanoh, Shin{\´i}chiro and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {Constant-phase-mode operation of the light-addressable potentiometric sensor}, series = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, journal = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1873-3077}, pages = {119 -- 123}, year = {2011}, language = {en} } @article{HaegerGrankinWagner2023, author = {Haeger, Gerrit and Grankin, Alina and Wagner, Michaela}, title = {Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology}, series = {Applied Research}, journal = {Applied Research}, number = {Early View}, publisher = {Wiley-VCH}, issn = {2702-4288}, doi = {10.1002/appl.202200106}, pages = {1 -- 15}, year = {2023}, abstract = {Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47\%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78\%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.}, language = {en} } @article{KrafftFoersterHartetal.2001, author = {Krafft, B. and F{\"o}rster, Arnold and Hart, A. van der and Sch{\"a}pers, T.}, title = {Control of Aharonov-Bohm oscillations in a AlGaAs/GaAs ring by asymmetric and symmetric gate biasing}, series = {Physica E: Low-dimensional Systems and Nanostructures. 9 (2001), H. 4}, journal = {Physica E: Low-dimensional Systems and Nanostructures. 9 (2001), H. 4}, isbn = {1386-9477}, pages = {635 -- 641}, year = {2001}, language = {en} } @article{ReisertGeisslerFlorkeetal.2011, author = {Reisert, Steffen and Geissler, H. and Florke, R. and Wagner, P. and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Controlling aseptic sterilization processes by means of a multi-sensor system}, publisher = {IEEE}, address = {New York}, pages = {18 -- 22}, year = {2011}, language = {en} } @article{MennickenPeterKaulenetal.2019, author = {Mennicken, Max and Peter, Sophia Katharina and Kaulen, Corinna and Simon, Ulrich and Karth{\"a}user, Silvia}, title = {Controlling the Electronic Contact at the Terpyridine/Metal Interface}, series = {The Journal of Physical Chemistry C}, volume = {123}, journal = {The Journal of Physical Chemistry C}, number = {35}, issn = {1932-7455}, doi = {10.1021/acs.jpcc.9b05865}, pages = {21367 -- 21375}, year = {2019}, language = {en} } @article{HennemannKohlReisertetal.2013, author = {Hennemann, J{\"o}rg and Kohl, Claus-Dieter and Reisert, Steffen and Kirchner, Patrick and Sch{\"o}ning, Michael Josef}, title = {Copper oxide nanofibres for detection of hydrogen peroxide vapour at high concentrations}, series = {physica status solidi (a)}, volume = {210}, journal = {physica status solidi (a)}, number = {5}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201200775}, pages = {859 -- 863}, year = {2013}, abstract = {We present a sensor concept based on copper(II)oxide (CuO) nanofibres for the detection of hydrogen peroxide (H2O2) vapour in the percent per volume (\% v/v) range. The fibres were produced by using the electrospinning technique. To avoid water condensation in the pores, the fibres were initially modified by an exposure to H2S to get an enclosed surface. By a thermal treatment at 350 °C the fibres were oxidised back to CuO. Thereby, the visible pores disappear which was verified by SEM analysis. The fibres show a decrease of resistance with increasing H2O2 concentration which is due to the fact that hydrogen peroxide is an oxidising gas and CuO a p-type semiconductor. The sensor shows a change of resistance within the minute range to the exposure until the maximum concentration of 6.9\% v/v H2O2. At operating temperatures below 450 °C the corresponding sensor response to a concentration of 4.1\% v/v increases. The sensor shows a good reproducibility of the signal at different measurements. CuO seems to be a suitable candidate for the detection of H2O2 vapour at high concentrations. Resistance behaviour of the sensor under exposure to H2O2 vapours between 2.3 and 6.9\% v/v at an operating temperature of 450 °C.}, language = {en} } @article{MourzinaSchoeningSchubertetal.2001, author = {Mourzina, Y. G. and Sch{\"o}ning, Michael Josef and Schubert, J. and Zander, W. and Legin, A. V. and Vlasov, Y. G. and L{\"u}th, H.}, title = {Copper, cadmium and thallium thin film sensors based on chalcogenide glasses}, series = {Analytica Chimica Acta. 433 (2001)}, journal = {Analytica Chimica Acta. 433 (2001)}, isbn = {0378-4304}, pages = {103 -- 110}, year = {2001}, language = {en} } @article{KraemerPitaZhouetal.2009, author = {Kr{\"a}mer, Melina and Pita, Marcos and Zhou, Jian and Ornatska, Maryna and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Katz, Evgeny}, title = {Coupling of Biocomputing Systems with Electronic Chips: Electronic Interface for Transduction of Biochemical Information}, series = {Journal of Physical Chemistry C: Nanomaterials and Interfaces. 113 (2009), H. 6}, journal = {Journal of Physical Chemistry C: Nanomaterials and Interfaces. 113 (2009), H. 6}, publisher = {American Cemical Society}, address = {Washington, DC}, isbn = {1932-7455}, pages = {2573 -- 2579}, year = {2009}, language = {en} }