@article{GlaserLubitzLovelandetal.2009, author = {Glaser, Stefan and Lubitz, Sandra and Loveland, Kate L. and Ohbo, Kazu and Robb, Lorraine and Schwenk, Frieder and Seibler, Jost and Roellig, Daniela and Kranz, Andrea and Anastassiadis, Konstantinos and Stewart, A. Francis}, title = {The histone 3 lysine 4 methyltransferase, Mll2, is only required briefly in development and spermatogenesis}, series = {Epigenetics \& Chromatin}, volume = {2}, journal = {Epigenetics \& Chromatin}, number = {5}, issn = {1756-8935}, doi = {10.1186/1756-8935-2-5}, pages = {1 -- 16}, year = {2009}, language = {en} } @article{KotnikPopovaTodirasetal.2009, author = {Kotnik, Katarina and Popova, Elena and Todiras, Mihail and Mori, Marcelo A. and Alenina, Natalia and Seibler, Jost and Bader, Michael}, title = {Inducible transgenic rat model for diabetes mellitus based on shRNA-mediated gene knockdown}, series = {Plos One}, volume = {4}, journal = {Plos One}, number = {4}, publisher = {Plos}, address = {San Francisco, California, US}, doi = {10.1371/journal.pone.0005124}, pages = {e5124}, year = {2009}, language = {en} } @article{HeroldBrandtSeibleretal.2008, author = {Herold, Marco J. and Brandt, Jens van den and Seibler, Jost and Reichardt, Holger M.}, title = {Inducible and reversible gene silencing by stable integration of an shRNA-encoding lentivirus in transgenic rats}, series = {Proceedings of the National Academy of Sciences of the United States of America}, volume = {105}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, number = {47}, issn = {1091-6490}, doi = {10.1073/pnas.0806213105}, pages = {18507 -- 18512}, year = {2008}, language = {en} } @article{KochWunderlichSeibleretal.2008, author = {Koch, Linda and Wunderlich, F. Thomas and Seibler, Jost and K{\"o}nner, A. Christine and Hampel, Brigitte and Irlenbusch, Sigrid and Brabant, Georg and Kahn, C. Ronald and Schwenk, Frieder and Br{\"u}ning, Jens C.}, title = {Central insulin action regulates peripheral glucose and fat metabolism in mice}, series = {The Journal of Clinical Investigation (JCI)}, volume = {118}, journal = {The Journal of Clinical Investigation (JCI)}, number = {6}, issn = {1558-8238}, doi = {10.1172/JCI31073}, pages = {2132 -- 2147}, year = {2008}, language = {en} } @article{ChristophBahrenbergVryetal.2008, author = {Christoph, Thomas and Bahrenberg, Gregor and Vry, Jean de and Englberger, Werner and Erdmann, Volker A. and Frech, Moritz and K{\"o}gel, Babette and R{\"o}hl, Thomas and Schiene, Klaus and Schr{\"o}der, Wolfgang and Seibler, Jost and Kurreck, Jens}, title = {Investigation of TRPV1 loss-of-function phenotypes in transgenic shRNA expressing and knockout mice}, series = {Molecular and Cellular Neuroscience}, volume = {37}, journal = {Molecular and Cellular Neuroscience}, number = {3}, issn = {1044-7431}, doi = {10.1016/j.mcn.2007.12.006}, pages = {579 -- 589}, year = {2008}, language = {en} } @article{CesariRennekampffVinterstenetal.2004, author = {Cesari, Francesca and Rennekampff, Verena and Vintersten, Kristina and Vuong, Lam Giang and Seibler, Jost and Bode, J{\"u}rgen and Wiebel, Franziska F. and Nordheim, Alfred}, title = {Elk-1 knock-out mice engineered by Flp recombinase-mediated cassette exchange}, series = {Genesis : The Journal of Genetics and Development}, volume = {38}, journal = {Genesis : The Journal of Genetics and Development}, number = {2}, issn = {1526-968X}, doi = {10.1002/gene.20003}, pages = {87 -- 92}, year = {2004}, language = {en} } @article{BodeSchlakeIberetal.2000, author = {Bode, J{\"u}rgen and Schlake, Thomas and Iber, Michaela and Sch{\"u}beler, Dirk and Seibler, Jost and Snezhkov, Evgeney and Nikolaev, Lev}, title = {The transgeneticist's toolbox: novel methods for the targeted modification of eukaryotic genomes}, series = {Biological Chemistry}, volume = {381}, journal = {Biological Chemistry}, number = {9-10}, issn = {1431-6730}, doi = {10.1515/BC.2000.103}, pages = {801 -- 813}, year = {2000}, language = {en} } @article{FengSeiblerAlamietal.1999, author = {Feng, Yong-Qing and Seibler, Jost and Alami, Raouf and Eisen, Andrew and Westerman, Karen A. and Leboulch, Philippe and Fiering, Steven and Bouhassira, Eric E.}, title = {Site-specific chromosomal integration in mammalian cells: highly efficient CRE recombinase-mediated cassette exchange}, series = {Journal of Molecular Biology}, volume = {292}, journal = {Journal of Molecular Biology}, number = {4}, issn = {0022-2836}, doi = {10.1006/jmbi.1999.3113}, pages = {779 -- 785}, year = {1999}, language = {en} } @article{SeiblerSchuebelerFieringetal.1998, author = {Seibler, Jost and Sch{\"u}beler, Dirk and Fiering, Steven and Groudine, Mark and Bode, J{\"u}rgen}, title = {DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs}, series = {Biochemistry}, volume = {37}, journal = {Biochemistry}, number = {18}, issn = {1520-4995}, doi = {10.1021/bi980288t}, pages = {6229 -- 6234}, year = {1998}, language = {en} } @article{SeiblerBode1997, author = {Seibler, Jost and Bode, J{\"u}rgen}, title = {Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay}, series = {Biochemistry}, volume = {36}, journal = {Biochemistry}, number = {7}, issn = {1520-4995}, pages = {1740 -- 1747}, year = {1997}, language = {en} } @article{BodeBartschBoulikasetal.1998, author = {Bode, J. and Bartsch, J. and Boulikas, T. and Iber, M. and Mielke, C. and Sch{\"u}beler, D. and Seibler, Jost and Benham, C.}, title = {Transcription-promoting genomic sites in mammalia: their elucidation and architectural principles}, series = {Gene therapy \& molecular biology}, volume = {1}, journal = {Gene therapy \& molecular biology}, number = {1}, issn = {1529-9120}, pages = {1 -- 29}, year = {1998}, language = {en} } @article{IberSchuebelerSeibleretal.1998, author = {Iber, Michaela and Sch{\"u}beler, Dirk and Seibler, Jost and H{\"o}xter, Maria and Bode, J{\"u}rgen}, title = {Efficient FACS selection procedure for cells undergoing Flp-mediated site-specific conversions}, series = {Technical Tips Online}, volume = {4}, journal = {Technical Tips Online}, number = {1}, doi = {10.1016/S1366-2120(08)70132-6}, pages = {25 -- 29}, year = {1998}, language = {en} } @article{HenkenOosterhuisOehlschlaegeretal.2012, author = {Henken, F. E. and Oosterhuis, K. and {\"O}hlschl{\"a}ger, Peter and Bosch, L. and Hooijberg, E. and Haanen, J. B. A. G. and Steenbergen, R. D. M.}, title = {Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7}, series = {Vaccine}, volume = {30}, journal = {Vaccine}, number = {28}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0264-410X}, doi = {10.1016/j.vaccine.2012.04.013}, pages = {4259 -- 4266}, year = {2012}, abstract = {Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of 'gene-shuffled' (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.}, language = {en} } @article{ImmelGruetzkeSpaeteetal.2012, author = {Immel, Timo and Gr{\"u}tzke, Martin and Sp{\"a}te, Anne-Katrin and Groth, Ulrich and {\"O}hlschl{\"a}ger, Peter and Huhn, Thomas}, title = {Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy}, series = {Chemical Communications}, volume = {48}, journal = {Chemical Communications}, number = {46}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {1364-548X}, doi = {10.1039/C2CC31624B}, pages = {5790 -- 5792}, year = {2012}, abstract = {Chelate stabilization of a titanium(IV)-salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model.}, language = {en} } @article{FellinKingKirschetal.2010, author = {Fellin, Wolfgang and King, Julian and Kirsch, Ansgar and Oberguggenberger, Michael}, title = {Uncertainty modelling and sensitivity analysis of tunnel face stability}, series = {Structural safety}, volume = {32}, journal = {Structural safety}, number = {6}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0167-4730}, doi = {10.1016/j.strusafe.2010.06.001}, pages = {402 -- 410}, year = {2010}, abstract = {This paper proposes an approach to the choice and evaluation of engineering models with the aid of a typical application in geotechnics. An important issue in the construction of shallow tunnels, especially in weak ground conditions, is the tunnel face stability. Various theoretical and numerical models for predicting the necessary support pressure have been put forth in the literature. In this paper, we combine laboratory experiments performed at the University of Innsbruck with current methods of uncertainty and sensitivity analysis for assessing adequacy, predictive power and robustness of the models. The major issues are the handling of the twofold uncertainty of test results and of model predictions as well as the decision about what are the influential input parameters.}, language = {en} } @article{Kirsch2010, author = {Kirsch, Ansgar}, title = {Experimental investigation of the face stability of shallow tunnels in sand}, series = {Acta Geotechnica}, volume = {5}, journal = {Acta Geotechnica}, number = {1}, publisher = {Springer}, address = {Berlin}, issn = {1861-1125}, doi = {10.1007/s11440-010-0110-7}, pages = {43 -- 62}, year = {2010}, abstract = {Various models have been proposed for the prediction of the necessary support pressure at the face of a shallow tunnel. To assess their quality, the collapse of a tunnel face was modelled with small-scale model tests at single gravity. The development of the failure mechanism and the support force at the face in dry sand were investigated. The observed displacement patterns show a negligible influence of overburden on the extent and evolution of the failure zone. The latter is significantly influenced, though, by the initial density of the sand: in dense sand a chimney-wedge-type collapse mechanism developed, which propagated towards the soil surface. Initially, loose sand did not show any discrete collapse mechanism. The necessary support force was neither influenced by the overburden nor the initial density. A comparison with quantitative predictions by several theoretical models showed that the measured necessary support pressure is overestimated by most of the models. Those by Vermeer/Ruse and L{\´e}ca/Dormieux showed the best agreement to the measurements.}, language = {en} } @inproceedings{KirschFelberMarcheretal.2014, author = {Kirsch, Ansgar and Felber, W. and Marcher, Thomas and Fuchs, W.}, title = {Geotechnical challenges during design and construction of a ski resort in Azerbaijan}, series = {Geotechnics of roads and railways : proceedings of the 15th Danube - European Conference on Geotechnical Engineering : 9-11 September 2014, Vienna, Austria}, booktitle = {Geotechnics of roads and railways : proceedings of the 15th Danube - European Conference on Geotechnical Engineering : 9-11 September 2014, Vienna, Austria}, publisher = {{\"O}IAV - {\"O}sterreichischer Ingenieur- und Architekten-Verein}, address = {Wien}, organization = {Danube - European Conference on Geotechnical Engineering <15, 2014, Wien>}, isbn = {978-3-902593-01-6}, pages = {757 -- 762}, year = {2014}, language = {en} } @article{KolymbasFellinKirsch2006, author = {Kolymbas, Dimitrios and Fellin, W. and Kirsch, Ansgar}, title = {Squeezing due to stress relaxation in foliated rock}, series = {International journal for numerical and analytical methods in geomechanics}, volume = {Vol. 30}, journal = {International journal for numerical and analytical methods in geomechanics}, number = {Iss. 13}, issn = {1096-9853 (E-Journal); 0363-9061 (Print)}, doi = {10.1002/nag.530}, pages = {1357 -- 1367}, year = {2006}, language = {en} } @article{ScheerSnaithWolfetal.2013, author = {Scheer, Nico and Snaith, Mike and Wolf, C. Roland and Seibler, Jost}, title = {Generation and utility of genetically humanized mouse models}, series = {Drug Discovery Today}, volume = {Vol 18}, journal = {Drug Discovery Today}, number = {23-24}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1359-6446}, doi = {10.1016/j.drudis.2013.07.007}, pages = {1200 -- 1211}, year = {2013}, language = {en} } @article{BouwmanGuldenHeijdenetal.2013, author = {Bouwman, Peter and Gulden, Hanneke van der and Heijden, Ingrid van der and Drost, Rinske and Klijn, Christiaan N. and Prasetyanti, Pramudita and Pieterse, Mark and Wientjens, Ellen and Seibler, Jost and Hogervorst, Frank B. L. and Jonkers, Jos}, title = {A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants}, series = {Cancer Discovery}, journal = {Cancer Discovery}, number = {3}, issn = {2159-8290}, doi = {10.1158/2159-8290.CD-13-0094}, pages = {1142 -- 1152}, year = {2013}, language = {en} } @article{MichalakNacerddinePietersenetal.2013, author = {Michalak, Ewa Malgorzata and Nacerddine, Karim and Pietersen, Alexandra and Beuger, Vincent and Pawlitzky, Inka and Cornelissen-Steijger, Paulien and Wientjens, Ellen and Tanger, Ellen and Seibler, Jost and Lohuizen, Maarten van and Jonkers, Jos}, title = {Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool}, series = {Stem Cells}, volume = {Vol 31}, journal = {Stem Cells}, number = {9}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1549-4918}, doi = {10.1002/stem.1437}, pages = {1910 -- 1920}, year = {2013}, language = {en} } @article{GebeshuberKornauthDongetal.2013, author = {Gebeshuber, Christoph A. and Kornauth, Christoph and Dong, Lihua and Sierig, Ralph and Seibler, Jost and Reiss, Martina and Tauber, Stefanie and Bilban, Martin and Wang, Shijun and Kain, Renate and B{\"o}hmig, Georg A. and Moeller, Marcus J. and Gr{\"o}ne, Hermann-Josef and Englert, Christoph and Martinez, Javier and Kerjaschki, Dontscho}, title = {Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1}, series = {Nature Medicine}, volume = {19}, journal = {Nature Medicine}, number = {4}, issn = {1078-8956}, doi = {10.1038/nm.3142}, pages = {481 -- 487}, year = {2013}, language = {en} } @article{KornfeldBaitzelKoenneretal.2013, author = {Kornfeld, Jan-Wilhelm and Baitzel, Catherina and K{\"o}nner, A. Christine and Nicholls, Hayley T. and Vogt, Merly C. and Herrmanns, Karolin and Scheja, Ludger and Haumaitre, C{\´e}cile and Wolf, Anna M. and Knippschild, Uwe and Seibler, Jost and Cereghini, Silvia and Heeren, Joerg and Stoffel, Markus and Br{\"u}ning, Jens C.}, title = {Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b}, series = {Nature}, volume = {494}, journal = {Nature}, number = {7435}, publisher = {Springer Nature}, address = {Cham}, isbn = {0028-0836}, doi = {10.1038/nature11793}, pages = {111 -- 115}, year = {2013}, language = {en} } @article{OberlaenderKirchnerKeusgenetal.2015, author = {Oberl{\"a}nder, Jan and Kirchner, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Strategies in developing thin-film sensors for monitoring aseptic food processes : Theoretical considerations and investigations of passivation materials}, series = {Electrochimica Acta}, volume = {183}, journal = {Electrochimica Acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2015.06.126}, pages = {130 -- 136}, year = {2015}, abstract = {The sterilization of packages in aseptic food processes is highly significant to maintain a consumer-safe product with extended shelf-life. Today, the sterilization of food packages is predominantly accomplished by gaseous hydrogen peroxide (H2O2) in combination with heat. In order to monitor this sterilization process, calorimetric gas sensors as differential set-up of two platinum temperature sensors representing a catalytically active (additionally deposition of MnO2) and a passive segment have been recently developed. The temperature rise of the exothermic decomposition serves as an indicator of the present H2O2 concentration. In the present work, a theoretical approach considering the sensor's thermochemistry and physical transport phenomena was formulated to evaluate the temperature rise based on the energy content of gaseous H2O2. In a further part of this work, three polymers have been analyzed with respect to their application as passivation materials. The examined polymers are photoresist SU-8, perfluoroalkoxy (PFA) and fluorinated ethylene propylene (FEP). Thermal analyses by means of differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) have been conducted to determine the operation limits of the polymers. The overall chemical resistance and stability of the polymers against the harsh environmental conditions during the sterilization process have been examined by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR).}, language = {en} } @inproceedings{NakagawaMichauxKallweitetal.2015, author = {Nakagawa, Masaki and Michaux, Frank and Kallweit, Stephan and Maeda, Kazuhiro}, title = {Unsteady flow measurements in the wake behind a wind-tunnel car model by using high-speed planar PIV}, series = {11TH International Symposium on Particle Image Velocimetry - PIV15 Santa Barbara, California, September 14-16, 2015}, booktitle = {11TH International Symposium on Particle Image Velocimetry - PIV15 Santa Barbara, California, September 14-16, 2015}, pages = {21 S.}, year = {2015}, abstract = {This study investigates unsteady characteristics of the wake behind a 28\%-scale car model in a wind tunnel using highspeed planar particle image velocimetry (PIV). The car model is based on a hatchback passenger car that is known to have relatively high fluctuations in its aerodynamic loads. This study primarily focuses on the lateral motion of the flow on the horizontal plane to determine the effect of the flow motion on the straight-line stability and the initial steering response of the actual car on a track. This paper first compares the flow fields in the wake behind the above mentioned model obtained using conventional and high-speed planar PIV, with sampling frequencies of 8 Hz and 1 kHz, respectively. Large asymmetrically coherent flow structures, which fluctuate at frequencies below 2 Hz, are observed in the results of highspeed PIV measurements, whereas conventional PIV is unable to capture these features of the flow owing to aliasing. This flow pattern with a laterally swaying motion is represented by opposite signs of cross-correlation coefficients of streamwise velocity fluctuations for the two sides of the car model. Effects of two aerodynamic devices that are known to reduce the fluctuation levels of the aerodynamic loads are then extensively investigated. The correlation analyses reveal that these devices indeed reduce the fluctuation levels of the flow and the correlation values around the rear combination-lamp, but it is found that the effects of these devices are different around the c-pillar.}, language = {en} } @inproceedings{BreuerRaueMangetal.2015, author = {Breuer, Lars and Raue, Markus and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, Ronald and Wagner, Torsten}, title = {Light-stimulated hydrogel actuators with incorporated graphene oxide for microfluidic applications}, series = {12. Dresdner Sensor-Symposium 2015}, booktitle = {12. Dresdner Sensor-Symposium 2015}, doi = {10.5162/12dss2015/P5.8}, pages = {206 -- 209}, year = {2015}, language = {en} } @inproceedings{OberlaenderJildehKirchneretal.2015, author = {Oberl{\"a}nder, Jan and Jildeh, Zaid B. and Kirchner, Patrick and Wendeler, Luisa and Bromm, Alexander and Iken, Heiko and Wagner, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Experimental and numerical evaluation of interdigitated electrode array for monitoring gaseous sterilization processes}, series = {12. Dresdner Sensor-Symposium 2015}, booktitle = {12. Dresdner Sensor-Symposium 2015}, doi = {10.5162/12dss2015/P3.11}, pages = {163 -- 168}, year = {2015}, language = {en} } @article{MiyamatoSakakitaWagneretal.2015, author = {Miyamato, Ko-ichiro and Sakakita, Sakura and Wagner, Torsten and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Application of chemical imaging sensor to in-situ pH imaging in the vicinity of a corroding metal surface}, series = {Electrochimica Acta}, volume = {183}, journal = {Electrochimica Acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2015.07.184}, pages = {137 -- 142}, year = {2015}, abstract = {The chemical imaging sensor was applied to in-situ pH imaging of the solution in the vicinity of a corroding surface of stainless steel under potentiostatic polarization. A test piece of polished stainless steel was placed on the sensing surface leaving a narrow gap filled with artificial seawater and the stainless steel was corroded under polarization. The pH images obtained during polarization showed correspondence between the region of lower pH and the site of corrosion. It was also found that the pH value in the gap became as low as 2 by polarization, which triggered corrosion.}, language = {en} } @article{HamadBilattoAdlyetal.2016, author = {Hamad, E. M. and Bilatto, S. E. R. and Adly, N. Y. and Correa, D. S. and Wolfrum, B. and Sch{\"o}ning, Michael Josef and Offenh{\"a}usser, A. and Yakushenko, A.}, title = {Inkjet printing of UV-curable adhesive and dielectric inks for microfluidic devices}, series = {Lab on a Chip}, volume = {16}, journal = {Lab on a Chip}, number = {1}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {1473-0189}, doi = {10.1039/C5LC01195G}, pages = {70 -- 74}, year = {2016}, abstract = {Bonding of polymer-based microfluidics to polymer substrates still poses a challenge for Lab-On-a-Chip applications. Especially, when sensing elements are incorporated, patterned deposition of adhesives with curing at ambient conditions is required. Here, we demonstrate a fabrication method for fully printed microfluidic systems with sensing elements using inkjet and stereolithographic 3D-printing.}, language = {en} } @inproceedings{RoosenFeyerl2015, author = {Roosen, Petra and Feyerl, G{\"u}nter}, title = {Gender-specific perception and utilization of personal use vehicles}, series = {FISITA World Automotive Congress 2014 : Maastricht, The Netherlands, 2 - 6 June / [organised by the International Federation of Automotive Engineering Societies (FISITA) ...]. Bd. 1}, booktitle = {FISITA World Automotive Congress 2014 : Maastricht, The Netherlands, 2 - 6 June / [organised by the International Federation of Automotive Engineering Societies (FISITA) ...]. Bd. 1}, publisher = {Curran}, address = {Red Hook, NY}, isbn = {978-1-5108-0209-4}, pages = {418 -- 425}, year = {2015}, language = {en} } @inproceedings{KilicRaatschenAstanehAsletal.2015, author = {Kilic, S. A. and Raatschen, Hans-J{\"u}rgen and Astaneh-Asl, A. and Apaydin, N. M.}, title = {Finite element modeling of the Fatih Sultan Mehmet Suspension Bridge}, series = {Multi-Span Large Bridges - Proceedings of the International Conference on Multi-Span Large Bridges}, booktitle = {Multi-Span Large Bridges - Proceedings of the International Conference on Multi-Span Large Bridges}, publisher = {CRC Press}, address = {Leiden}, isbn = {9781138027572}, pages = {1169 -- 1173}, year = {2015}, language = {en} } @article{FischerSelverGezeretal.2015, author = {Fischer, Felix and Selver, M. Alper and Gezer, Sinem and Dicle, Oguz and Hillen, Walter}, title = {Systematic Parameterization, Storage, and Representation of Volumetric DICOM Data}, series = {Journal of Medical and Biological Engineering}, volume = {35}, journal = {Journal of Medical and Biological Engineering}, number = {6}, publisher = {Springer}, address = {Berlin}, issn = {2199-4757}, doi = {10.1007/s40846-015-0097-5}, pages = {709 -- 723}, year = {2015}, language = {en} } @inproceedings{HorikawaKazariOkadaetal.2015, author = {Horikawa, Atsushi and Kazari, Masahide and Okada, Kunio and Funke, Harald and Keinz, Jan and Kusterer, Karsten and Haji Ayed, Anis}, title = {Developments of Hydrogen Dry Low Emission Combustion Technology}, series = {Annual Congress of Gas Turbine Society Japan, 2015}, booktitle = {Annual Congress of Gas Turbine Society Japan, 2015}, pages = {5 S.}, year = {2015}, language = {en} } @inproceedings{Wollert2015, author = {Wollert, J{\"o}rg}, title = {Wireless systems for machinery safety : Requirements and solutions for wireless real time systems}, series = {16th International Conference on Research and Education in Mechatronics (REM), 2015}, booktitle = {16th International Conference on Research and Education in Mechatronics (REM), 2015}, isbn = {978-3-945728-01-7}, doi = {10.1109/REM.2015.7380377}, pages = {88 -- 91}, year = {2015}, language = {en} } @inproceedings{NakagawaKallweitMichauxetal.2016, author = {Nakagawa, Masaki and Kallweit, Stephan and Michaux, Frank and Hojo, Teppei}, title = {Typical Velocity Fields and Vortical Structures around a Formula One Car, based on Experimental Investigations using Particle Image Velocimetry}, series = {SAE International Journal of Passenger Cars - Mechanical Systems}, booktitle = {SAE International Journal of Passenger Cars - Mechanical Systems}, issn = {1946-4002}, doi = {10.4271/2016-01-1611}, pages = {18 S.}, year = {2016}, language = {en} } @article{BayonValeroGarciaBartualetal.2016, author = {Bayon, Arnau and Valero, Daniel and Garcia-Bartual, Rafael and Vall{\´e}s-Mor{\´a}n, Francisco Jos{\´e} and L{\´o}pez-Jim{\´e}nez, P. Amparo}, title = {Performance assessment of OpenFOAM and FLOW-3D in the numerical modeling of a low Reynolds number hydraulic jump}, series = {Environmental Modelling \& Software}, volume = {80}, journal = {Environmental Modelling \& Software}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1364-8152}, doi = {10.1016/j.envsoft.2016.02.018}, pages = {322 -- 335}, year = {2016}, abstract = {A comparative performance analysis of the CFD platforms OpenFOAM and FLOW-3D is presented, focusing on a 3D swirling turbulent flow: a steady hydraulic jump at low Reynolds number. Turbulence is treated using RANS approach RNG k-ε. A Volume Of Fluid (VOF) method is used to track the air-water interface, consequently aeration is modeled using an Eulerian-Eulerian approach. Structured meshes of cubic elements are used to discretize the channel geometry. The numerical model accuracy is assessed comparing representative hydraulic jump variables (sequent depth ratio, roller length, mean velocity profiles, velocity decay or free surface profile) to experimental data. The model results are also compared to previous studies to broaden the result validation. Both codes reproduced the phenomenon under study concurring with experimental data, although special care must be taken when swirling flows occur. Both models can be used to reproduce the hydraulic performance of energy dissipation structures at low Reynolds numbers.}, language = {en} } @article{DantismTakenagaWagneretal.2016, author = {Dantism, Shahriar and Takenaga, Shoko and Wagner, Patrick and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Determination of the extracellular acidification of Escherichia coli K12 with a multi-​chamber-​based LAPS system}, series = {Physica status solidi (a)}, volume = {213}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300}, doi = {10.1002/pssa.201533043}, pages = {1479 -- 1485}, year = {2016}, abstract = {On-line monitoring of the metabolic activity of microorganisms involved in intermediate stages of biogas production plays an important role to avoid undesirable "down times" during the biogas production. In order to control this process, an on-chip differential measuring system based on the light-addressable potentiometric sensor (LAPS) principle combined with a 3D-printed multi-chamber structure has been realized. As a test microorganism, Escherichia coli K12 (E. coli K12) were used for cell-based measurements. Multi-chamber structures were developed to determine the metabolic activity of E. coli K12 in suspension for a different number of cells, responding to the addition of a constant or variable amount of glucose concentrations, enabling differential and simultaneous measurements.}, language = {en} } @inproceedings{SchreiberKraftZuendorf2016, author = {Schreiber, Marc and Kraft, Bodo and Z{\"u}ndorf, Albert}, title = {Cost-efficient quality assurance of natural language processing tools through continuous monitoring with continuous integration}, series = {3rd International Workshop on Software Engineering Research and Industrial Practice}, booktitle = {3rd International Workshop on Software Engineering Research and Industrial Practice}, doi = {10.1145/2897022.2897029}, pages = {46 -- 52}, year = {2016}, language = {en} } @inproceedings{Huening2016, author = {H{\"u}ning, Felix}, title = {Power Semiconductors for the automotive 48V board net}, series = {PCIM Europe 2016 Conference Proceedings}, booktitle = {PCIM Europe 2016 Conference Proceedings}, publisher = {VDE Verl.}, address = {Berlin}, isbn = {978-3-8007-4186-1}, pages = {1963 -- 1969}, year = {2016}, language = {en} } @article{BreuerRaueStrobeletal.2016, author = {Breuer, Lars and Raue, Markus and Strobel, M. and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, R. and Wagner, Torsten}, title = {Hydrogels with incorporated graphene oxide as light-addressable actuator materials for cell culture environments in lab-on-chip systems}, series = {Physica status solidi (a)}, volume = {213}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300}, doi = {10.1002/pssa.201533056}, pages = {1520 -- 1525}, year = {2016}, abstract = {Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.}, language = {en} } @article{PookhalilAmoabedinyTabeshetal.2016, author = {Pookhalil, Ali and Amoabediny, Ghassem and Tabesh, Hadi and Behbahani, Mehdi and Mottaghy, Khosrow}, title = {A new approach for semiempirical modeling of mechanical blood trauma}, series = {The international journal of artificial organs}, volume = {39}, journal = {The international journal of artificial organs}, number = {4}, publisher = {Sage}, address = {London}, issn = {1724-6040}, doi = {10.5301/ijao.5000474}, pages = {171 -- 177}, year = {2016}, abstract = {Purpose Two semi-empirical models were recently published, both making use of existing literature data, but each taking into account different physical phenomena that trigger hemolysis. In the first model, hemoglobin (Hb) release is described as a permeation procedure across the membrane, assuming a shear stress-dependent process (sublethal model). The second model only accounts for hemoglobin release that is caused by cell membrane breakdown, which occurs when red blood cells (RBC) undergo mechanically induced shearing for a period longer than the threshold time (nonuniform threshold model). In this paper, we introduce a model that considers the hemolysis generated by both these possible phenomena. Methods Since hemolysis can possibly be caused by permeation of hemoglobin through the RBC functional membrane as well as by release of hemoglobin from RBC membrane breakdown, our proposed model combines both these models. An experimental setup consisting of a Couette device was utilized for validation of our proposed model. Results A comparison is presented between the damage index (DI) predicted by the proposed model vs. the sublethal model vs. the nonthreshold model and experimental datasets. This comparison covers a wide range of shear stress for both human and porcine blood. An appropriate agreement between the measured DI and the DI predicted by the present model was obtained. Conclusions The semiempirical hemolysis model introduced in this paper aims for significantly enhanced conformity with experimental data. Two phenomenological outcomes become possible with the proposed approach: an estimation of the average time after which cell membrane breakdown occurs under the applied conditions, and a prediction of the ratio between the phenomena involved in hemolysis.}, language = {en} } @book{GebhardtHoetter2016, author = {Gebhardt, Andreas and H{\"o}tter, Jan-Steffen}, title = {Additive manufacturing : 3D printing for prototyping and manufacturing}, publisher = {Hanser Publishers}, address = {Munich}, isbn = {978-1-56990-582-1 ; 978-1-56990-583-8}, pages = {591 S.}, year = {2016}, language = {en} } @inproceedings{KasperSchiffelsKrafftetal.2016, author = {Kasper, Katharina and Schiffels, Johannes and Krafft, Simone and Kuperjans, Isabel and Elbers, Gereon and Selmer, Thorsten}, title = {Biogas Production on Demand Regulated by Butyric Acid Addition}, series = {IOP Conference Series: Earth and Environmental Science. Bd. 32}, volume = {32}, booktitle = {IOP Conference Series: Earth and Environmental Science. Bd. 32}, issn = {1755-1315}, doi = {10.1088/1755-1315/32/1/012009}, pages = {012009/1 -- 012009/4}, year = {2016}, language = {en} } @article{BertzSchoeningMolinnusetal.2024, author = {Bertz, Morten and Sch{\"o}ning, Michael Josef and Molinnus, Denise and Homma, Takayuki}, title = {Influence of temperature, light, and H₂O₂ concentration on microbial spore inactivation: in-situ Raman spectroscopy combined with optical trapping}, series = {Physica status solidi (a) applications and materials science}, journal = {Physica status solidi (a) applications and materials science}, number = {Early View}, publisher = {Wiley-VCH}, address = {Berlin}, issn = {1862-6319 (Online)}, doi = {10.1002/pssa.202300866}, pages = {8 Seiten}, year = {2024}, abstract = {To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30\% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.}, language = {en} } @article{SchoenrockMuckeltHastermannetal.2024, author = {Schoenrock, Britt and Muckelt, Paul E. and Hastermann, Maria and Albracht, Kirsten and MacGregor, Robert and Martin, David and Gunga, Hans-Christian and Salanova, Michele and Stokes, Maria J. and Warner, Martin B. and Blottner, Dieter}, title = {Muscle stiffness indicating mission crew health in space}, series = {Scientific Reports}, volume = {14}, journal = {Scientific Reports}, number = {Article number: 4196}, publisher = {Springer Nature}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-024-54759-6}, pages = {13 Seiten}, year = {2024}, abstract = {Muscle function is compromised by gravitational unloading in space affecting overall musculoskeletal health. Astronauts perform daily exercise programmes to mitigate these effects but knowing which muscles to target would optimise effectiveness. Accurate inflight assessment to inform exercise programmes is critical due to lack of technologies suitable for spaceflight. Changes in mechanical properties indicate muscle health status and can be measured rapidly and non-invasively using novel technology. A hand-held MyotonPRO device enabled monitoring of muscle health for the first time in spaceflight (> 180 days). Greater/maintained stiffness indicated countermeasures were effective. Tissue stiffness was preserved in the majority of muscles (neck, shoulder, back, thigh) but Tibialis Anterior (foot lever muscle) stiffness decreased inflight vs. preflight (p < 0.0001; mean difference 149 N/m) in all 12 crewmembers. The calf muscles showed opposing effects, Gastrocnemius increasing in stiffness Soleus decreasing. Selective stiffness decrements indicate lack of preservation despite daily inflight countermeasures. This calls for more targeted exercises for lower leg muscles with vital roles as ankle joint stabilizers and in gait. Muscle stiffness is a digital biomarker for risk monitoring during future planetary explorations (Moon, Mars), for healthcare management in challenging environments or clinical disorders in people on Earth, to enable effective tailored exercise programmes.}, language = {en} } @techreport{Drescher2021, type = {Working Paper}, author = {Drescher, Hans Paul}, title = {Turbulence - minimum dissipation and maximum macroscopic momentum exchange}, pages = {44 Seiten}, year = {2021}, abstract = {The minimum dissipation requirement of the thermodynamics of irreversible processes is applied to characterize the existence of laminar and non-laminar, and the co-existence of laminar and turbulent flow zones. Local limitations of the different zones and three different forms of transition are defined. For the Couette flow a non-local "corpuscular" flow mechanism explains the logarithmic law-of-the-wall, maximum turbulent dimensions and a value x=0,415 for the v. K{\´a}rm{\´a}n constant. Limitations of the logarithmic law near the wall and in the centre of the experiment are interpreted.}, language = {en} } @techreport{Drescher2018, type = {Working Paper}, author = {Drescher, Hans Paul}, title = {The irreversible thermodynamic's theorem of minimum entropy production applied to the laminar and the turbulent Couette flow}, pages = {48 Seiten}, year = {2018}, abstract = {Analyzing thermodynamic non-equilibrium processes, like the laminar and turbulent fluid flow, the dissipation is a key parameter with a characteristic minimum condition. That is applied to characterize laminar and turbulent behaviour of the Couette flow, including its transition in both directions. The Couette flow is chosen as the only flow form with constant shear stress over the flow profile, being laminar, turbulent or both. The local dissipation defines quantitative and stable criteria for the transition and the existence of turbulence. There are basic results: The Navier Stokes equations cannot describe the experimental flow profiles of the turbulent Couette flow. But they are used to quantify the dissipation of turbulent fluctuation. The dissipation minimum requires turbulent structures reaching maximum macroscopic dimensions, describing turbulence as a "non-local" phenomenon. At the transition the Couette flow profiles and the shear stress change by a factor ≅ 5 due to a change of the "apparent" turbulent viscosity by a calculated factor ≅ 27. The resulting difference of the laminar and the turbulent profiles results in two different Reynolds numbers and different loci of transition, which are identified by calculation.}, language = {en} } @incollection{SchneiderWisselinkCzarneckietal.2024, author = {Schneider, Dominik and Wisselink, Frank and Czarnecki, Christian and N{\"o}lle, Nikolai}, title = {Benefits and framework conditions for information-driven business models concerning the Internet of Things}, series = {Digitalization in companies}, booktitle = {Digitalization in companies}, publisher = {Springer}, address = {Wiesbaden}, isbn = {978-3-658-39093-8 (Print)}, doi = {10.1007/978-3-658-39094-5_5}, pages = {59 -- 75}, year = {2024}, abstract = {In the context of the increasing digitalization, the Internet of Things (IoT) is seen as a technological driver through which completely new business models can emerge in the interaction of different players. Identified key players include traditional industrial companies, municipalities and telecommunications companies. The latter, by providing connectivity, ensure that small devices with tiny batteries can be connected almost anywhere and directly to the Internet. There are already many IoT use cases on the market that provide simplification for end users, such as Philips Hue Tap. In addition to business models based on connectivity, there is great potential for information-driven business models that can support or enhance existing business models. One example is the IoT use case Park and Joy, which uses sensors to connect parking spaces and inform drivers about available parking spaces in real time. Information-driven business models can be based on data generated in IoT use cases. For example, a telecommunications company can add value by deriving more decision-relevant information - called insights - from data that is used to increase decision agility. In addition, insights can be monetized. The monetization of insights can only be sustainable, if careful attention is taken and frameworks are considered. In this chapter, the concept of information-driven business models is explained and illustrated with the concrete use case Park and Joy. In addition, the benefits, risks and framework conditions are discussed.}, language = {en} } @article{PolenKraemerBongaertsetal.2005, author = {Polen, T. and Kr{\"a}mer, Marco and Bongaerts, Johannes and Wubbolts, Marcel and Wendisch, V. F.}, title = {The global gene expression response of Escherichia coli to L-phenylalanine}, series = {Journal of biotechnology}, volume = {Vol. 115}, journal = {Journal of biotechnology}, number = {Iss. 3}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, pages = {221 -- 237}, year = {2005}, language = {en} } @article{BongaertsKraemerMuelleretal.2001, author = {Bongaerts, Johannes and Kr{\"a}mer, Marco and M{\"u}ller, Ulrike and Raeven, Leon and Wubbolts, Marcel}, title = {Metabolic engineering for microbial production of aromatic amino acids and derived compounds}, series = {Metabolic engineering}, volume = {Vol. 3}, journal = {Metabolic engineering}, number = {Iss. 4}, issn = {1096-7184 (E-Journal); 1096-7176 (Print)}, pages = {289 -- 300}, year = {2001}, language = {en} }