@article{GrinsvenBonStrauvenetal.2012, author = {Grinsven, Bart van and Bon, Natalie vanden and Strauven, Hannelore and Grieten, Lars and Murib, Mohammed and Jim{\´e}nez Monroy, Kathia L. and Janssens, Stoffel D. and Haenen, Ken and Sch{\"o}ning, Michael Josef and Vermeeren, Veronique and Ameloot, Marcel and Michiels, Luc and Thoelen, Ronald and Ceuninck, Ward de and Wagner, Patrick}, title = {Heat-Transfer Resistance at Solid-Liquid Interfaces: A Tool for The Detection of Single Nucleotide Polymorphisms in DNA.}, series = {ACS Nano}, volume = {6}, journal = {ACS Nano}, number = {3}, publisher = {ACS Publications}, address = {Washington, DC}, issn = {1936-086X}, doi = {10.1021/nn300147e}, pages = {2712 -- 2721}, year = {2012}, abstract = {In this article, we report on the heat-transfer resistance at interfaces as a novel, denaturation-based method to detect single-nucleotide polymorphisms in DNA. We observed that a molecular brush of double-stranded DNA grafted onto synthetic diamond surfaces does not notably affect the heat-transfer resistance at the solid-to-liquid interface. In contrast to this, molecular brushes of single-stranded DNA cause, surprisingly, a substantially higher heat-transfer resistance and behave like a thermally insulating layer. This effect can be utilized to identify ds-DNA melting temperatures via the switching from low- to high heat-transfer resistance. The melting temperatures identified with this method for different DNA duplexes (29 base pairs without and with built-in mutations) correlate nicely with data calculated by modeling. The method is fast, label-free (without the need for fluorescent or radioactive markers), allows for repetitive measurements, and can also be extended toward array formats. Reference measurements by confocal fluorescence microscopy and impedance spectroscopy confirm that the switching of heat-transfer resistance upon denaturation is indeed related to the thermal on-chip denaturation of DNA.}, language = {en} } @article{MuribYeapEurlingsetal.2016, author = {Murib, M. S. and Yeap, W. S. and Eurlings, Y. and Grinsven, B. van and Boyen, H.-G. and Conings, B. and Michiels, L. and Ameloot, M. and Carleer, R. and Warmer, J. and Kaul, P. and Haenen, K. and Sch{\"o}ning, Michael Josef and Ceuninck, W. de and Wagner, P.}, title = {Heat-transfer based characterization of DNA on synthetic sapphire chips}, series = {Sensors and Actuators B: Chemical}, volume = {230}, journal = {Sensors and Actuators B: Chemical}, number = {230}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2016.02.027}, pages = {260 -- 271}, year = {2016}, abstract = {In this study, we show that synthetic sapphire (Al₂O₃), an established implant material, can also serve as a platform material for biosensors comparable to nanocrystalline diamond. Sapphire chips, beads, and powder were first modified with (3-aminopropyl) triethoxysilane (APTES), followed by succinic anhydride (SA), and finally single-stranded probe DNA was EDC coupled to the functionalized layer. The presence of the APTES-SA layer on sapphire powders was confirmed by thermogravimetric analyis and Fourier-transform infrared spectroscopy. Using planar sapphire chips as substrates and X-ray photoelectron spectroscopy (XPS) as surface-sensitive tool, the sequence of individual layers was analyzed with respect to their chemical state, enabling the quantification of areal densities of the involved molecular units. Fluorescence microscopy was used to demonstrate the hybridization of fluorescently tagged target DNA to the probe DNA, including denaturation- and re-hybridization experiments. Due to its high thermal conductivity, synthetic sapphire is especially suitable as a chip material for the heat-transfer method, which was employed to distinguish complementary- and non-complementary DNA duplexes containing single-nucleotide polymorphisms. These results indicate that it is possible to detect mutations electronically with a chemically resilient and electrically insulating chip material.}, language = {en} } @article{WagnerMolinaYoshinobuetal.2007, author = {Wagner, Torsten and Molina, Roberto and Yoshinobu, Tatsuo and Kloock, Joachim P. and Biselli, Manfred and Canzoneri, Michele and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef}, title = {Handheld multi-channel LAPS device as a transducer platform for possible biological and chemical multi-sensor applications}, series = {Electrochimica Acta. 53 (2007), H. 2}, journal = {Electrochimica Acta. 53 (2007), H. 2}, isbn = {0013-4686}, pages = {305 -- 311}, year = {2007}, language = {en} } @article{WagnerMarisAckermannetal.2007, author = {Wagner, Torsten and Maris, Rob J. and Ackermann, Hans-Josef and Otto, Ralph and Beging, Stefan and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Handheld measurement device for field-effect sensor structures: Practical evaluation and limitations}, series = {Sensors and Actuators B: Chemical . 127 (2007), H. 1}, journal = {Sensors and Actuators B: Chemical . 127 (2007), H. 1}, isbn = {0925-4005}, pages = {217 -- 223}, year = {2007}, language = {en} } @article{PoghossianBaeckerMayeretal.2015, author = {Poghossian, Arshak and B{\"a}cker, Matthias and Mayer, Dirk and Sch{\"o}ning, Michael Josef}, title = {Gating capacitive field-effect sensors by the charge of nanoparticle/molecule hybrids}, series = {Nanoscale}, journal = {Nanoscale}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, issn = {2040-3372 (E-Journal); 2040-3364 (Print)}, doi = {10.1039/C4NR05987E}, pages = {1023 -- 1031}, year = {2015}, language = {en} } @article{SchoeningKirchnerNgetal.2010, author = {Sch{\"o}ning, Michael Josef and Kirchner, Patrick and Ng, Yue Ann and Spelthahn, Heiko and Schneider, Andreas and Henkel, Hartmut and Friedrich, Peter and Kolstad, Jens and Berger, J{\"o}rg and Keusgen, Michael}, title = {Gas sensor investigation based on a catalytically activated thin-film thermopile for H2O2 detection}, series = {Physica Status Solidi (A). 207 (2010), H. 4}, journal = {Physica Status Solidi (A). 207 (2010), H. 4}, isbn = {1862-6300}, pages = {787 -- 792}, year = {2010}, language = {en} } @article{BussEckenWinkelsetal.2000, author = {Buß, G. and Ecken, H. and Winkels, S. and Sch{\"o}ning, Michael Josef and L{\"u}th, H. and Schultze, J. W.}, title = {Galvanic modifications of multifunctional silicon-based microelectrode arrays}, series = {Electrochemical technology applications in electronics : proceedings of the third international symposium / [International Symposium on Electrochemical Technology Applications in Electronics, held during October 20 - 22, 1999, Honolulu, Hawaii]. Electrodeposition Division. Ed.: L. T. Romankiw}, journal = {Electrochemical technology applications in electronics : proceedings of the third international symposium / [International Symposium on Electrochemical Technology Applications in Electronics, held during October 20 - 22, 1999, Honolulu, Hawaii]. Electrodeposition Division. Ed.: L. T. Romankiw}, publisher = {Electrochemical Society}, address = {Pennington, NJ}, isbn = {1566772575}, pages = {209 -- 215}, year = {2000}, language = {en} } @inproceedings{PoghossianSchumacherKloocketal.2006, author = {Poghossian, Arshak and Schumacher, Kerstin and Kloock, Joachim P. and Rosenkranz, Christian and Schultze, Joachim W. and M{\"u}ller-Veggian, Mattea and Sch{\"o}ning, Michael Josef}, title = {Functional testing and characterisation of ISFETs on wafer level by means of a micro-droplet cell}, url = {http://nbn-resolving.de/urn:nbn:de:hbz:a96-opus-1259}, year = {2006}, abstract = {A wafer-level functionality testing and characterisation system for ISFETs (ionsensitive field-effect transistor) is realised by means of integration of a specifically designed capillary electrochemical micro-droplet cell into a commercial wafer prober-station. The developed system allows the identification and selection of "good" ISFETs at the earliest stage and to avoid expensive bonding, encapsulation and packaging processes for nonfunctioning ISFETs and thus, to decrease costs, which are wasted for bad dies. The developed system is also feasible for wafer-level characterisation of ISFETs in terms of sensitivity, hysteresis and response time. Additionally, the system might be also utilised for wafer-level testing of further electrochemical sensors.}, subject = {Biosensor}, language = {en} } @article{PoghossianWagnerSchoening2009, author = {Poghossian, Arshak and Wagner, Holger and Sch{\"o}ning, Michael Josef}, title = {Functional testing and characterisation of (bio-)chemical sensors on wafer level}, series = {Procedia Chemistry. 1 (2009), H. 1}, journal = {Procedia Chemistry. 1 (2009), H. 1}, isbn = {1876-6196}, pages = {835 -- 838}, year = {2009}, language = {en} } @article{PoghossianWagnerSchoening2011, author = {Poghossian, Arshak and Wagner, Holger and Sch{\"o}ning, Michael Josef}, title = {Functional testing and characterisation of (bio-)chemical sensors on wafer level}, series = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, journal = {Sensors and Actuators B: Chemical. 154 (2011), H. 2}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1873-3077}, pages = {169 -- 173}, year = {2011}, language = {en} } @article{WernerWagnerYoshinobuetal.2013, author = {Werner, Frederik and Wagner, Torsten and Yoshinobu, Tatsuo and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Frequency behaviour of light-addressable potentiometric sensors}, series = {Physica Status Solidi (A)}, volume = {210}, journal = {Physica Status Solidi (A)}, number = {5}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1521-396X ; 0031-8965}, doi = {10.1002/pssa.201200929}, pages = {884 -- 891}, year = {2013}, abstract = {Light-addressable potentiometric sensors (LAPS) are semiconductor-based potentiometric sensors, with the advantage to detect the concentration of a chemical species in a liquid solution above the sensor surface in a spatially resolved manner. The addressing is achieved by a modulated and focused light source illuminating the semiconductor and generating a concentration-depending photocurrent. This work introduces a LAPS set-up that is able to monitor the electrical impedance in addition to the photocurrent. The impedance spectra of a LAPS structure, with and without illumination, as well as the frequency behaviour of the LAPS measurement are investigated. The measurements are supported by electrical equivalent circuits to explain the impedance and the LAPS-frequency behaviour. The work investigates the influence of different parameters on the frequency behaviour of the LAPS. Furthermore, the phase shift of the photocurrent, the influence of the surface potential as well as the changes of the sensor impedance will be discussed.}, language = {en} } @article{WagnerWernerMiyamotoetal.2010, author = {Wagner, Torsten and Werner, Frederik and Miyamoto, Ko-ichiro and Ackermann, Hans-Josef and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {FPGA-based LAPS device for the flexible design of sensing sites on functional interfaces}, series = {Physica Status Solidi (A). 207 (2010), H. 4}, journal = {Physica Status Solidi (A). 207 (2010), H. 4}, isbn = {1862-6300}, pages = {844 -- 849}, year = {2010}, language = {en} } @article{PoghossianYoshinobuSchoening2003, author = {Poghossian, Arshak and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {Flow-velocity microsensors based on semiconductor field-effect structures}, series = {Sensors. 3 (2003), H. 7}, journal = {Sensors. 3 (2003), H. 7}, isbn = {1424-8220}, pages = {202 -- 212}, year = {2003}, language = {en} } @article{WangKrauseBlocketal.2003, author = {Wang, J. and Krause, R. and Block, K. and Musameh, M. and Mulchandani, A. and Sch{\"o}ning, Michael Josef}, title = {Flow injection amperometric detection of OP nerve agents based on organophosphorus-hydrolase biosensor detector}, series = {Biosensors \& Bioelectronics. 18 (2003), H. 2-3}, journal = {Biosensors \& Bioelectronics. 18 (2003), H. 2-3}, isbn = {0956-5663}, pages = {255 -- 260}, year = {2003}, language = {en} } @inproceedings{OberlaenderKirchnerKeusgenetal.2014, author = {Oberl{\"a}nder, Jan and Kirchner, Patrick and Keusgen, M. and Sch{\"o}ning, Michael Josef}, title = {Flexible polyimide-based calorimetric gas sensors for monitoring hy-drogen peroxide in sterilisation processes of aseptic filling machines}, series = {Sensoren und Messsysteme 2014 ; Beitr{\"a}ge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in N{\"u}rnberg. (ITG-Fachbericht ; 250)}, booktitle = {Sensoren und Messsysteme 2014 ; Beitr{\"a}ge der 17. GMA/ITG-Fachtagung vom 3. bis 4. Juni 2014 in N{\"u}rnberg. (ITG-Fachbericht ; 250)}, publisher = {VDE-Verl.}, address = {D{\"u}sseldorf}, organization = {VDI/VDE-Gesellschaft Mess- und Automatisierungstechnik}, isbn = {978-3-8007-3622-5}, pages = {1 -- 4}, year = {2014}, language = {en} } @article{WagnerMiyamotoWerneretal.2011, author = {Wagner, Torsten and Miyamoto, K. and Werner, Frederik and Sch{\"o}ning, Michael Josef and Yoshinobu, T.}, title = {Flexible electrochemical imaging with "zoom-in" functionality by using a new type of light-addressable potentiometric sensor}, publisher = {IEEE}, address = {New York}, pages = {2133 -- 2135}, year = {2011}, language = {en} } @article{VahidpourOberlaenderSchoening2018, author = {Vahidpour, Farnoosh and Oberl{\"a}nder, Jan and Sch{\"o}ning, Michael Josef}, title = {Flexible Calorimetric Gas Sensors for Detection of a Broad Concentration Range of Gaseous Hydrogen Peroxide: A Step Forward to Online Monitoring of Food-Package Sterilization Processes}, series = {Phys. Status Solidi A}, volume = {215}, journal = {Phys. Status Solidi A}, number = {15}, publisher = {Wiley-VCH}, address = {Weinheim}, doi = {10.1002/pssa.201800044}, pages = {Artikel 1800044}, year = {2018}, abstract = {In this study, flexible calorimetric gas sensors are developed for specificdetection of gaseous hydrogen peroxide (H₂O₂) over a wide concentrationrange, which is used in sterilization processes for aseptic packaging industry.The flexibility of these sensors is an advantage for identifying the chemical components of the sterilant on the corners of the food boxes, so-called "coldspots", as critical locations in aseptic packaging, which are of great importance. These sensors are fabricated on flexible polyimide films by means of thin-film technique. Thin layers of titanium and platinum have been deposited on polyimide to define the conductive structures of the sensors. To detect the high-temperature evaporated H₂O₂, a differential temperature set-up is proposed. The sensors are evaluated in a laboratory-scaled sterilizationsystem to simulate the sterilization process. The concentration range of the evaporated H₂O₂ from 0 to 7.7\% v/v was defined and the sensors have successfully detected high as well as low H₂O₂ concentrations with a sensitivity of 5.04 °C/\% v/v. The characterizations of the sensors confirm their precise fabrication, high sensitivity and the novelty of low H₂O₂ concentration detections for future inline monitoring of food-package sterilization.}, language = {en} } @article{WernerSchusserSpalthahnetal.2011, author = {Werner, Frederik and Schusser, Sebastian and Spalthahn, Heiko and Wagner, Torsten and Yoshinobu, Tatsuo and Sch{\"o}ning, Michael Josef}, title = {Field-programmable gate array based controller for multi spot light-addressable potentiometric sensors with integrated signal correction mode}, series = {Electrochimica Acta}, volume = {56}, journal = {Electrochimica Acta}, number = {26}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2011.03.012}, pages = {9656 -- 9660}, year = {2011}, abstract = {A light-addressable potentiometric sensor (LAPS) can measure the concentration of one or several analytes at the sensor surface simultaneously in a spatially resolved manner. A modulated light pointer stimulates the semiconductor structure at the area of interest and a responding photocurrent can be read out. By simultaneous stimulation of several areas with light pointers of different modulation frequencies, the read out can be performed at the same time. With the new proposed controller electronic based on a field-programmable gate array (FPGA), it is possible to control the modulation frequencies, phase shifts, and light brightness of multiple light pointers independently and simultaneously. Thus, it is possible to investigate the frequency response of the sensor, and to examine the analyte concentration by the determination of the surface potential with the help of current/voltage curves and phase/voltage curves. Additionally, the ability to individually change the light intensities of each light pointer is used to perform signal correction.}, language = {en} } @article{PoghossianAbouzarAmbergeretal.2007, author = {Poghossian, Arshak and Abouzar, Maryam H. and Amberger, F. and Mayer, D. and Han, Y. and Ingebrandt, S. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef}, title = {Field-effect sensors with charged macromolecules: Characterisation by capacitance-voltage, constant-capacitance, impedance spectroscopy and atomic-force microscopy methods}, series = {Biosensors and Bioelectronics. 22 (2007), H. 9-10}, journal = {Biosensors and Bioelectronics. 22 (2007), H. 9-10}, isbn = {0956-5663}, pages = {2100 -- 2107}, year = {2007}, language = {en} } @inproceedings{PoghossianIngebrandtPlatenetal.2006, author = {Poghossian, Arshak and Ingebrandt, S. and Platen, J. and Sch{\"o}ning, Michael Josef}, title = {Field-effect sensors with charged macromolecules - from micro towards nano aspects}, series = {Biochemical Sensing Utilisation of Micro-and Nanotechnologies, Warschau, Nov. 2005 : Lecture Notes of the ICB Seminar / ed.: M. Mascini, W. Torbicz}, booktitle = {Biochemical Sensing Utilisation of Micro-and Nanotechnologies, Warschau, Nov. 2005 : Lecture Notes of the ICB Seminar / ed.: M. Mascini, W. Torbicz}, publisher = {Polish Academy Sciences Press}, address = {Warsaw}, pages = {74 -- 81}, year = {2006}, language = {en} } @article{PoghossianJablonskiMolinnusetal.2020, author = {Poghossian, Arshak and Jablonski, Melanie and Molinnus, Denise and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers}, series = {Frontiers in Plant Science}, volume = {11}, journal = {Frontiers in Plant Science}, number = {Article 598103}, publisher = {Frontiers}, address = {Lausanne}, doi = {10.3389/fpls.2020.598103}, pages = {1 -- 14}, year = {2020}, abstract = {Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases.}, language = {en} } @article{PoghossianAbouzarSakkarietal.2006, author = {Poghossian, Arshak and Abouzar, Maryam H. and Sakkari, M. and Kassab, T. and Han, Y. and Ingebrandt, S. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef}, title = {Field-effect sensors for monitoring the layer-by-layer adsorption of charged macromolecules}, series = {Sensors and Actuators B: Chemical. 118 (2006), H. 1-2}, journal = {Sensors and Actuators B: Chemical. 118 (2006), H. 1-2}, isbn = {0925-4005}, pages = {163 -- 170}, year = {2006}, language = {en} } @article{YoshinobuMiyamotoWagneretal.2024, author = {Yoshinobu, Tatsuo and Miyamoto, Ko-ichiro and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Field-effect sensors combined with the scanned light pulse technique: from artificial olfactory images to chemical imaging technologies}, series = {Chemosensors}, volume = {12}, journal = {Chemosensors}, number = {2}, publisher = {MDPI}, address = {Basel}, issn = {2227-9040}, doi = {10.3390/chemosensors12020020}, pages = {Artikel 20}, year = {2024}, abstract = {The artificial olfactory image was proposed by Lundstr{\"o}m et al. in 1991 as a new strategy for an electronic nose system which generated a two-dimensional mapping to be interpreted as a fingerprint of the detected gas species. The potential distribution generated by the catalytic metals integrated into a semiconductor field-effect structure was read as a photocurrent signal generated by scanning light pulses. The impact of the proposed technology spread beyond gas sensing, inspiring the development of various imaging modalities based on the light addressing of field-effect structures to obtain spatial maps of pH distribution, ions, molecules, and impedance, and these modalities have been applied in both biological and non-biological systems. These light-addressing technologies have been further developed to realize the position control of a faradaic current on the electrode surface for localized electrochemical reactions and amperometric measurements, as well as the actuation of liquids in microfluidic devices.}, language = {en} } @article{AbouzarIngebrandtPoghossianetal.2009, author = {Abouzar, Maryam H. and Ingebrandt, S. and Poghossian, Arshak and Zhang, Y. and Moritz, W. and Sch{\"o}ning, Michael Josef}, title = {Field-effect nanoplate capacitive pH sensor based on SOI structure}, series = {Semiconductor micro- and nanoelectronics : Proceedings of the Seventh International Conference , Tsakhcadzor, Armenia July 3-5 2009}, journal = {Semiconductor micro- and nanoelectronics : Proceedings of the Seventh International Conference , Tsakhcadzor, Armenia July 3-5 2009}, pages = {55 -- 58}, year = {2009}, language = {en} } @article{GunSchoeningAbouzaretal.2008, author = {Gun, Jenny and Sch{\"o}ning, Michael Josef and Abouzar, Maryam H. and Poghossian, Arshak and Katz, Evgeny}, title = {Field-Effect Nanoparticle-Based Glucose Sensor on a Chip: Amplification Effect of Coimmobilized Redox Species}, series = {Electroanalysis. 20 (2008), H. 16}, journal = {Electroanalysis. 20 (2008), H. 16}, isbn = {1521-4109}, pages = {1748 -- 1753}, year = {2008}, language = {en} } @article{PoghossianWeilBaeckeretal.2012, author = {Poghossian, Arshak and Weil, M. H. and B{\"a}cker, Matthias and Mayer, D. and Sch{\"o}ning, Michael Josef}, title = {Field-effect Devices Functionalised with Gold-Nanoparticle/Macromolecule Hybrids: New Opportunities for a Label-Free Biosensing}, series = {Procedia Engineering}, journal = {Procedia Engineering}, number = {47}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2012.09.136}, pages = {273 -- 276}, year = {2012}, abstract = {Field-effect capacitive electrolyte-insulator-semiconductor (EIS) sensors functionalised with citrate-capped gold nanoparticles (AuNP) have been used for the electrostatic detection of macromolecules by their intrinsic molecular charge. The EIS sensor detects the charge changes in the AuNP/macromolecule hybrids induced by the adsorption or binding events. A feasibility of the proposed detection scheme has been exemplary demonstrated by realising EIS sensors for the detection of poly-D-lysine molecules.}, language = {en} } @article{PoghossianIngebrandtOffenhaeusseretal.2009, author = {Poghossian, Arshak and Ingebrandt, S. and Offenh{\"a}usser, A. and Sch{\"o}ning, Michael Josef}, title = {Field-effect devices for detecting cellular signals}, series = {Seminars in Cell \& Developmental Biology. 20 (2009), H. 1}, journal = {Seminars in Cell \& Developmental Biology. 20 (2009), H. 1}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1096-3634}, pages = {41 -- 48}, year = {2009}, language = {en} } @article{BegingMlynekHataihimakuletal.2010, author = {Beging, Stefan and Mlynek, Daniela and Hataihimakul, Sudkanung and Poghossian, Arshak and Baldsiefen, Gerhard and Busch, Heinz and Laube, Norbert and Kleinen, Lisa and Sch{\"o}ning, Michael Josef}, title = {Field-effect calcium sensor for the determination of the risk of urinary stone formation}, series = {Sensors and Actuators B: Chemical. 144 (2010), H. 2}, journal = {Sensors and Actuators B: Chemical. 144 (2010), H. 2}, pages = {374 -- 379}, year = {2010}, language = {en} } @inproceedings{JablonskiKochBronderetal.2017, author = {Jablonski, Melanie and Koch, Claudia and Bronder, Thomas and Poghossian, Arshak and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Field-Effect Biosensors Modified with Tobacco Mosaic Virus Nanotubes as Enzyme Nanocarrier}, series = {MDPI Proceeding}, volume = {1}, booktitle = {MDPI Proceeding}, number = {4}, doi = {10.3390/proceedings1040505}, pages = {4}, year = {2017}, language = {en} } @article{PoghossianJablonskiKochetal.2018, author = {Poghossian, Arshak and Jablonski, Melanie and Koch, Claudia and Bronder, Thomas and Rolka, David and Wege, Christina and Sch{\"o}ning, Michael Josef}, title = {Field-effect biosensor using virus particles as scaffolds for enzyme immobilization}, series = {Biosensors and Bioelectronics}, volume = {110}, journal = {Biosensors and Bioelectronics}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0956-5663}, doi = {10.1016/j.bios.2018.03.036}, pages = {168 -- 174}, year = {2018}, abstract = {A field-effect biosensor employing tobacco mosaic virus (TMV) particles as scaffolds for enzyme immobilization is presented. Nanotubular TMV scaffolds allow a dense immobilization of precisely positioned enzymes with retained activity. To demonstrate feasibility of this new strategy, a penicillin sensor has been developed by coupling a penicillinase with virus particles as a model system. The developed field-effect penicillin biosensor consists of an Al-p-Si-SiO₂-Ta₂O₅-TMV structure and has been electrochemically characterized in buffer solutions containing different concentrations of penicillin G. In addition, the morphology of the biosensor surface with virus particles was characterized by scanning electron microscopy and atomic force microscopy methods. The sensors possessed a high penicillin sensitivity of ~ 92 mV/dec in a nearly-linear range from 0.1 mM to 10 mM, and a low detection limit of about 50 µM. The long-term stability of the penicillin biosensor was periodically tested over a time period of about one year without any significant loss of sensitivity. The biosensor has also been successfully applied for penicillin detection in bovine milk samples.}, language = {en} } @article{SchoeningPoghossianSchultzeetal.2002, author = {Sch{\"o}ning, Michael Josef and Poghossian, Arshak and Schultze, J. W. and L{\"u}th, H.}, title = {Field-effect based multifunctional hybrid sensor module for the determination of both (bio-)chemical and physical paramters}, series = {Proceedings of SPIE. 4576 (2002)}, journal = {Proceedings of SPIE. 4576 (2002)}, pages = {149 -- 159}, year = {2002}, language = {en} } @article{SchuetzWeissbeckerHummeletal.1997, author = {Sch{\"u}tz, S. and Weißbecker, B. and Hummel, Hans E. and Sch{\"o}ning, Michael Josef and Riemer, A. and Kordos, P. and L{\"u}th, H.}, title = {Field effect transistor-insect antenna junction}, series = {Naturwissenschaften}, volume = {84}, journal = {Naturwissenschaften}, issn = {1432-1904}, pages = {86 -- 88}, year = {1997}, language = {en} } @article{JildehKirchnerOberlaenderetal.2017, author = {Jildeh, Zaid B. and Kirchner, Patrick and Oberl{\"a}nder, Jan and Kremers, Alexander and Wagner, Torsten and Wagner, Patrick H. and Sch{\"o}ning, Michael Josef}, title = {FEM-based modeling of a calorimetric gas sensor for hydrogen peroxide monitoring}, series = {physica status solidi a : applications and materials sciences}, journal = {physica status solidi a : applications and materials sciences}, number = {Early View}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201600912}, year = {2017}, abstract = {A physically coupled finite element method (FEM) model is developed to study the response behavior of a calorimetric gas sensor. The modeled sensor serves as a monitoring device of the concentration of gaseous hydrogen peroxide (H2 O2) in a high temperature mixture stream in aseptic sterilization processes. The principle of operation of a calorimetric H2 O2 sensor is analyzed and the results of the numerical model have been validated by using previously published sensor experiments. The deviation in the results between the FEM model and experimental data are presented and discussed.}, language = {en} } @article{WendlandtKochBritzetal.2023, author = {Wendlandt, Tim and Koch, Claudia and Britz, Beate and Liedek, Anke and Schmidt, Nora and Werner, Stefan and Gleba, Yuri and Vahidpour, Farnoosh and Welden, Melanie and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System}, series = {Viruses}, volume = {9}, journal = {Viruses}, number = {15}, publisher = {MDPI}, address = {Basel}, issn = {1999-4915}, doi = {doi.org/10.3390/v15091951}, pages = {Artikel 1951}, year = {2023}, abstract = {Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes.}, language = {en} } @article{YoshinobuSchoeningFingeretal.2004, author = {Yoshinobu, T. and Sch{\"o}ning, Michael Josef and Finger, F. and Moritz, W. and Iwasaki, H.}, title = {Fabrication of thin-film LAPS with amorphous silicon}, series = {Sensors. 4 (2004), H. 10}, journal = {Sensors. 4 (2004), H. 10}, isbn = {1424-8220}, pages = {163 -- 169}, year = {2004}, language = {en} } @article{MuckWangJacobsetal.2004, author = {Muck, A. and Wang, J. and Jacobs, M. and Chen, G. and Chatrathi, M. P. and Jurka, V. and Vyborny, Z. and Spillmann, S. D. and Sridharan, G. and Sch{\"o}ning, Michael Josef}, title = {Fabrication of poly(methyl methacrylate) microfluidic chips by atmospheric molding}, series = {Analytical Chemistry. 76 (2004), H. 8}, journal = {Analytical Chemistry. 76 (2004), H. 8}, isbn = {0003-2700}, pages = {2290 -- 2297}, year = {2004}, language = {en} } @article{TakenagaSchneiderErbayetal.2015, author = {Takenaga, Shoko and Schneider, Benno and Erbay, E. and Biselli, Manfred and Schnitzler, Thomas and Sch{\"o}ning, Michael Josef and Wagner, Torsten}, title = {Fabrication of biocompatible lab-on-chip devices for biomedical applications by means of a 3D-printing process}, series = {Physica status solidi (a)}, volume = {212}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201532053}, pages = {1347 -- 1352}, year = {2015}, abstract = {A new microfluidic assembly method for semiconductor-based biosensors using 3D-printing technologies was proposed for a rapid and cost-efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D-printer in just a few hours and assembled on a light-addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics-based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D-printed chips. Furthermore, an optimal cell culturing within microfluidics-based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.}, language = {en} } @article{SchrothSchoeningLuethetal.2001, author = {Schroth, P. and Sch{\"o}ning, Michael Josef and L{\"u}th, H. and Weißbecker, B. and Hummel, Hans E. and Sch{\"u}tz, S.}, title = {Extending the capabilities of an antenna/chip biosensor by employing various insect species}, series = {Sensors and Actuators B. 78 (2001), H. 1-3}, journal = {Sensors and Actuators B. 78 (2001), H. 1-3}, isbn = {0925-4005}, pages = {1 -- 5}, year = {2001}, language = {en} } @article{SchrothSchoeningLuethetal.2000, author = {Schroth, P. and Sch{\"o}ning, Michael Josef and L{\"u}th, H. and Weißbecker, B. and Hummel, Hans E. and Sch{\"u}tz, S.}, title = {Extending the capabilities of an antenna/chip biosensor by employing various insect species}, series = {Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]}, journal = {Proceedings : Copenhagen, Denmark, 27 - 30 August 2000 / [ed.: R. de Reus ...]}, publisher = {MIC, Mikroelektronik Centret}, address = {Lyngby, Denmark}, isbn = {87-89935-50-0}, pages = {27 -- 30}, year = {2000}, language = {en} } @inproceedings{OberlaenderJildehKirchneretal.2015, author = {Oberl{\"a}nder, Jan and Jildeh, Zaid B. and Kirchner, Patrick and Wendeler, Luisa and Bromm, Alexander and Iken, Heiko and Wagner, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Experimental and numerical evaluation of interdigitated electrode array for monitoring gaseous sterilization processes}, series = {12. Dresdner Sensor-Symposium 2015}, booktitle = {12. Dresdner Sensor-Symposium 2015}, doi = {10.5162/12dss2015/P3.11}, pages = {163 -- 168}, year = {2015}, language = {en} } @article{JildehOberlaenderKirchneretal.2018, author = {Jildeh, Zaid B. and Oberl{\"a}nder, Jan and Kirchner, Patrick and Keusgen, Michael and Wagner, Patrick H. and Sch{\"o}ning, Michael Josef}, title = {Experimental and Numerical Analyzes of a Sensor Based on Interdigitated Electrodes for Studying Microbiological Alterations}, series = {physica status solidi (a): applications and materials science}, volume = {215}, journal = {physica status solidi (a): applications and materials science}, number = {15}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6319}, doi = {10.1002/pssa.201700920}, pages = {Artikel 1700920}, year = {2018}, abstract = {In this work, a cell-based biosensor to evaluate the sterilization efficacy of hydrogen peroxide vapor sterilization processes is characterized. The transducer of the biosensor is based on interdigitated gold electrodes fabricated on an inert glass substrate. Impedance spectroscopy is applied to evaluate the sensor behavior and the alteration of test microorganisms due to the sterilization process. These alterations are related to changes in relative permittivity and electrical conductivity of the bacterial spores. Sensor measurements are conducted with and without bacterial spores (Bacillus atrophaeus), as well as after an industrial sterilization protocol. Equivalent two-dimensional numerical models based on finite element method of the periodic finger structures of the interdigitated gold electrodes are designed and validated using COMSOL® Multiphysics software by the application of known dielectric properties. The validated models are used to compute the electrical properties at different sensor states (blank, loaded with spores, and after sterilization). As a final result, we will derive and tabulate the frequency-dependent electrical parameters of the spore layer using a novel model that combines experimental data with numerical optimization techniques.}, language = {en} } @article{SchoeningSimonisKringsetal.2002, author = {Sch{\"o}ning, Michael Josef and Simonis, A. and Krings, T. and L{\"u}th, H. and Wang, J.}, title = {Evaluation of a chip-based thin-film / thick-film sensor hybrid for (bio-)chemical analysis}, series = {Electroanalysis}, volume = {14}, journal = {Electroanalysis}, number = {13}, issn = {1040-0397}, pages = {955 -- 958}, year = {2002}, language = {en} } @article{BohrnStuetzFleischeretal.2011, author = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef and Wagner, Patrick}, title = {Eukaryotic cell lines as a sensitive layer for direct monitoring of carbon monoxide}, series = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, journal = {Physica status solidi (a) : applications and material science. 208 (2011), H. 6}, publisher = {Wiley}, address = {Weinheim}, isbn = {1862-6319}, pages = {1345 -- 1350}, year = {2011}, language = {en} } @inproceedings{BuniatyanHuckPoghossianetal.2014, author = {Buniatyan, V. V. and Huck, Christina and Poghossian, Arshak and Sch{\"o}ning, Michael Josef and Rustamyan, L. G. and Hovnikyan, H. H.}, title = {Equivalent circuit and optimization of impedance characteristics of an electrolyte conductivity sensor}, series = {Proceedings of State Engineering University Armenia : Series Information technologies, electronics, radio engineering}, volume = {Iss. 17}, booktitle = {Proceedings of State Engineering University Armenia : Series Information technologies, electronics, radio engineering}, number = {No. 1}, pages = {69 -- 76}, year = {2014}, language = {en} } @article{BandodkarMolinnusMirzaetal.2014, author = {Bandodkar, Amay J. and Molinnus, Denise and Mirza, Omar and Guinovart, Tomas and Windmiller, Joshua R. and Valdes-Ramirez, Gabriela and Andrade, Francisco J. and Sch{\"o}ning, Michael Josef and Wang, Joseph}, title = {Epidermal tattoo potentiometric sodium sensors with wireless signal transduction for continuous non-invasive sweat monitoring}, series = {Biosensors and bioelectronics}, volume = {54}, journal = {Biosensors and bioelectronics}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4235 (E-Journal); 0956-5663 (Print)}, doi = {10.1016/j.bios.2013.11.039}, pages = {603 -- 609}, year = {2014}, abstract = {This article describes the fabrication, characterization and application of an epidermal temporary-transfer tattoo-based potentiometric sensor, coupled with a miniaturized wearable wireless transceiver, for real-time monitoring of sodium in the human perspiration. Sodium excreted during perspiration is an excellent marker for electrolyte imbalance and provides valuable information regarding an individual's physical and mental wellbeing. The realization of the new skin-worn non-invasive tattoo-like sensing device has been realized by amalgamating several state-of-the-art thick film, laser printing, solid-state potentiometry, fluidics and wireless technologies. The resulting tattoo-based potentiometric sodium sensor displays a rapid near-Nernstian response with negligible carryover effects, and good resiliency against various mechanical deformations experienced by the human epidermis. On-body testing of the tattoo sensor coupled to a wireless transceiver during exercise activity demonstrated its ability to continuously monitor sweat sodium dynamics. The real-time sweat sodium concentration was transmitted wirelessly via a body-worn transceiver from the sodium tattoo sensor to a notebook while the subjects perspired on a stationary cycle. The favorable analytical performance along with the wearable nature of the wireless transceiver makes the new epidermal potentiometric sensing system attractive for continuous monitoring the sodium dynamics in human perspiration during diverse activities relevant to the healthcare, fitness, military, healthcare and skin-care domains.}, language = {en} } @article{TurekKeusgenPoghossianetal.2008, author = {Turek, M. and Keusgen, M. and Poghossian, Arshak and Mulchandani, A. and Wang, J. and Sch{\"o}ning, Michael Josef}, title = {Enzyme-modified electrolyte-insulator-semiconductor sensors}, series = {Journal of Contemporary Physics. 43 (2008), H. 2}, journal = {Journal of Contemporary Physics. 43 (2008), H. 2}, isbn = {1934-9378}, pages = {82 -- 85}, year = {2008}, language = {en} } @article{KatzPoghossianSchoening2017, author = {Katz, Evgeny and Poghossian, Arshak and Sch{\"o}ning, Michael Josef}, title = {Enzyme-based logic gates and circuits - analytical applications and interfacing with electronics}, series = {Analytical and Bioanalytical Chemistry}, volume = {409}, journal = {Analytical and Bioanalytical Chemistry}, publisher = {Springer}, address = {Berlin}, issn = {1618-2650}, doi = {10.1007/s00216-016-0079-7}, pages = {81 -- 94}, year = {2017}, abstract = {The paper is an overview of enzyme-based logic gates and their short circuits, with specific examples of Boolean AND and OR gates, and concatenated logic gates composed of multi-step enzyme-biocatalyzed reactions. Noise formation in the biocatalytic reactions and its decrease by adding a "filter" system, converting convex to sigmoid response function, are discussed. Despite the fact that the enzyme-based logic gates are primarily considered as components of future biomolecular computing systems, their biosensing applications are promising for immediate practical use. Analytical use of the enzyme logic systems in biomedical and forensic applications is discussed and exemplified with the logic analysis of biomarkers of various injuries, e.g., liver injury, and with analysis of biomarkers characteristic of different ethnicity found in blood samples on a crime scene. Interfacing of enzyme logic systems with modified electrodes and semiconductor devices is discussed, giving particular attention to the interfaces functionalized with signal-responsive materials. Future perspectives in the design of the biomolecular logic systems and their applications are discussed in the conclusion.}, language = {en} } @article{PoghossianKatzSchoening2015, author = {Poghossian, Arshak and Katz, Evgeny and Sch{\"o}ning, Michael Josef}, title = {Enzyme logic AND-Reset and OR-Reset gates based on a field-effect electronic transducer modified with multi-enzyme membrane}, series = {Chemical Communications}, volume = {51}, journal = {Chemical Communications}, publisher = {Royal Society of Chemistry (RSC)}, address = {Cambridge}, doi = {10.1039/C5CC01362C}, pages = {6564 -- 6567}, year = {2015}, abstract = {Capacitive field-effect sensors modified with a multi-enzyme membrane have been applied for an electronic transduction of biochemical signals processed by enzyme-based AND-Reset and OR-Reset logic gates. The local pH change at the sensor surface induced by the enzymatic reaction was used for the activation of the Reset function for the first time.}, language = {en} } @article{ThustSchoeningSchrothetal.1999, author = {Thust, M. and Sch{\"o}ning, Michael Josef and Schroth, P. and Malkoc, {\"U}. and Dicker, C. I. and Steffen, A. and Kordos, P. and L{\"u}th, H.}, title = {Enzyme immobilisation on planar and porous silicon substrates for biosensor applications}, series = {Journal of Molecular Catalysis B: Enzymatic. 7 (1999), H. 1-4}, journal = {Journal of Molecular Catalysis B: Enzymatic. 7 (1999), H. 1-4}, isbn = {1381-1177}, pages = {77 -- 83}, year = {1999}, language = {en} } @article{SchusserBaeckerKrischeretal.2014, author = {Schusser, Sebastian and B{\"a}cker, Matthias and Krischer, M. and Wenzel, L. and Leinhos, Marcel and Poghossian, Arshak and Biselli, Manfred and Wagner, P. and Sch{\"o}ning, Michael Josef}, title = {Enzymatically catalyzed degradation of biodegradable polymers investigated by means of a semiconductor-based field-effect sensor}, series = {Procedia Engineering}, volume = {87}, journal = {Procedia Engineering}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1877-7058}, doi = {10.1016/j.proeng.2014.11.689}, pages = {1314 -- 1317}, year = {2014}, abstract = {A semiconductor field-effect device has been used for an enzymatically catalyzed degradation of biopolymers for the first time. This novel technique is capable to monitor the degradation process of multiple samples in situ and in real-time. As model system, the degradation of the biopolymer poly(D, L-lactic acid) has been monitored in the degradation medium containing the enzyme lipase from Rhizomucor miehei. The obtained results demonstrate the potential of capacitive field-effect sensors for degradation studies of biodegradable polymers.}, language = {en} }