@article{SeiblerSchuebelerFieringetal.1998, author = {Seibler, Jost and Sch{\"u}beler, Dirk and Fiering, Steven and Groudine, Mark and Bode, J{\"u}rgen}, title = {DNA cassette exchange in ES cells mediated by Flp recombinase: an efficient strategy for repeated modification of tagged loci by marker-free constructs}, series = {Biochemistry}, volume = {37}, journal = {Biochemistry}, number = {18}, issn = {1520-4995}, doi = {10.1021/bi980288t}, pages = {6229 -- 6234}, year = {1998}, language = {en} } @article{SeiblerBode1997, author = {Seibler, Jost and Bode, J{\"u}rgen}, title = {Double-reciprocal crossover mediated by FLP-recombinase: a concept and an assay}, series = {Biochemistry}, volume = {36}, journal = {Biochemistry}, number = {7}, issn = {1520-4995}, pages = {1740 -- 1747}, year = {1997}, language = {en} } @article{IberSchuebelerSeibleretal.1998, author = {Iber, Michaela and Sch{\"u}beler, Dirk and Seibler, Jost and H{\"o}xter, Maria and Bode, J{\"u}rgen}, title = {Efficient FACS selection procedure for cells undergoing Flp-mediated site-specific conversions}, series = {Technical Tips Online}, volume = {4}, journal = {Technical Tips Online}, number = {1}, doi = {10.1016/S1366-2120(08)70132-6}, pages = {25 -- 29}, year = {1998}, language = {en} } @article{HenkenOosterhuisOehlschlaegeretal.2012, author = {Henken, F. E. and Oosterhuis, K. and {\"O}hlschl{\"a}ger, Peter and Bosch, L. and Hooijberg, E. and Haanen, J. B. A. G. and Steenbergen, R. D. M.}, title = {Preclinical safety evaluation of DNA vaccines encoding modified HPV16 E6 and E7}, series = {Vaccine}, volume = {30}, journal = {Vaccine}, number = {28}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0264-410X}, doi = {10.1016/j.vaccine.2012.04.013}, pages = {4259 -- 4266}, year = {2012}, abstract = {Persistent infection with high-risk human papillomaviruses (hrHPV) can result in the formation of anogenital cancers. As hrHPV proteins E6 and E7 are required for cancer initiation and maintenance, they are ideal targets for immunotherapeutic interventions. Previously, we have described the development of DNA vaccines for the induction of HPV16 E6 and E7 specific T cell immunity. These vaccines consist of 'gene-shuffled' (SH) versions of HPV16 E6 and E7 that were fused to Tetanus Toxin Fragment C domain 1 (TTFC) and were named TTFC-E6SH and TTFC-E7SH. Gene-shuffling was performed to avoid the risk of inducing malignant transformation at the vaccination site. Here, we describe the preclinical safety evaluation of these candidate vaccines by analysis of their transforming capacity in vitro using established murine fibroblasts (NIH 3T3 cells) and primary human foreskin keratinocytes (HFKs). We demonstrate that neither ectopic expression of TTFC-E6SH and TTFC-E7SH alone or in combination enabled NIH 3T3 cells to form colonies in soft agar. In contrast, expression of HPV16 E6WT and E7WT alone or in combination resulted in effective transformation. Similarly, retroviral transduction of HFKs from three independent donors with both TTFC-E6SH and TTFC-E7SH alone or in combination did not show any signs of immortalization. In contrast, the combined expression of E6WT and E7WT induced immortalization in HFKs from all donors. Based on these results we consider it justified to proceed to clinical evaluation of DNA vaccines encoding TTFC-E6SH and TTFC-E7SH in patients with HPV16 associated (pre)malignancies.}, language = {en} } @article{ImmelGruetzkeSpaeteetal.2012, author = {Immel, Timo and Gr{\"u}tzke, Martin and Sp{\"a}te, Anne-Katrin and Groth, Ulrich and {\"O}hlschl{\"a}ger, Peter and Huhn, Thomas}, title = {Synthesis and X-ray structure analysis of a heptacoordinate titanium(IV)-bis-chelate with enhanced in vivo antitumor efficacy}, series = {Chemical Communications}, volume = {48}, journal = {Chemical Communications}, number = {46}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {1364-548X}, doi = {10.1039/C2CC31624B}, pages = {5790 -- 5792}, year = {2012}, abstract = {Chelate stabilization of a titanium(IV)-salan alkoxide by ligand exchange with 2,6-pyridinedicarboxylic acid (dipic) resulted in heptacoordinate complex 3 which is not redox-active, stable on silica gel and has increased aqueous stability. 3 is highly toxic in HeLa S3 and Hep G2 and has enhanced antitumor efficacy in a mouse cervical-cancer model.}, language = {en} } @article{FellinKingKirschetal.2010, author = {Fellin, Wolfgang and King, Julian and Kirsch, Ansgar and Oberguggenberger, Michael}, title = {Uncertainty modelling and sensitivity analysis of tunnel face stability}, series = {Structural safety}, volume = {32}, journal = {Structural safety}, number = {6}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0167-4730}, doi = {10.1016/j.strusafe.2010.06.001}, pages = {402 -- 410}, year = {2010}, abstract = {This paper proposes an approach to the choice and evaluation of engineering models with the aid of a typical application in geotechnics. An important issue in the construction of shallow tunnels, especially in weak ground conditions, is the tunnel face stability. Various theoretical and numerical models for predicting the necessary support pressure have been put forth in the literature. In this paper, we combine laboratory experiments performed at the University of Innsbruck with current methods of uncertainty and sensitivity analysis for assessing adequacy, predictive power and robustness of the models. The major issues are the handling of the twofold uncertainty of test results and of model predictions as well as the decision about what are the influential input parameters.}, language = {en} } @article{Kirsch2010, author = {Kirsch, Ansgar}, title = {Experimental investigation of the face stability of shallow tunnels in sand}, series = {Acta Geotechnica}, volume = {5}, journal = {Acta Geotechnica}, number = {1}, publisher = {Springer}, address = {Berlin}, issn = {1861-1125}, doi = {10.1007/s11440-010-0110-7}, pages = {43 -- 62}, year = {2010}, abstract = {Various models have been proposed for the prediction of the necessary support pressure at the face of a shallow tunnel. To assess their quality, the collapse of a tunnel face was modelled with small-scale model tests at single gravity. The development of the failure mechanism and the support force at the face in dry sand were investigated. The observed displacement patterns show a negligible influence of overburden on the extent and evolution of the failure zone. The latter is significantly influenced, though, by the initial density of the sand: in dense sand a chimney-wedge-type collapse mechanism developed, which propagated towards the soil surface. Initially, loose sand did not show any discrete collapse mechanism. The necessary support force was neither influenced by the overburden nor the initial density. A comparison with quantitative predictions by several theoretical models showed that the measured necessary support pressure is overestimated by most of the models. Those by Vermeer/Ruse and L{\´e}ca/Dormieux showed the best agreement to the measurements.}, language = {en} } @article{KolymbasKirsch2009, author = {Kolymbas, Dimitrios and Kirsch, Ansgar}, title = {Zum Erddruck auf Lawinenschutzgalerien}, series = {Bautechnik : Zeitschrift f{\"u}r den gesamten Ingenieurbau}, volume = {Vol. 86}, journal = {Bautechnik : Zeitschrift f{\"u}r den gesamten Ingenieurbau}, number = {Iss. 12}, issn = {1437-0999 (E-Journal); 0005-6820 (Print); 0932-8351 (Print)}, doi = {10.1002/bate.200910075}, pages = {774 -- 779}, year = {2009}, language = {de} } @article{KirschPiazzi2009, author = {Kirsch, Ansgar and Piazzi, Lukas}, title = {Numerical investigation of the effectiveness of a bored pile wall for the minimisation of settlement resulting from tunnel driving}, series = {Geomechanics and tunnelling}, volume = {Vol. 2}, journal = {Geomechanics and tunnelling}, number = {Iss. 6}, issn = {1865-7362}, doi = {10.1002/geot.200900063}, pages = {753 -- 765}, year = {2009}, language = {de} } @article{Kirsch2008, author = {Kirsch, Ansgar}, title = {Experimentelle Untersuchung des Ortsbrustversagens seichter Tunnel}, series = {{\"O}sterreichische Bauwirtschaft}, journal = {{\"O}sterreichische Bauwirtschaft}, number = {Sondernr.}, pages = {6 -- 9}, year = {2008}, language = {de} } @article{KolymbasFellinKirsch2006, author = {Kolymbas, Dimitrios and Fellin, W. and Kirsch, Ansgar}, title = {Squeezing due to stress relaxation in foliated rock}, series = {International journal for numerical and analytical methods in geomechanics}, volume = {Vol. 30}, journal = {International journal for numerical and analytical methods in geomechanics}, number = {Iss. 13}, issn = {1096-9853 (E-Journal); 0363-9061 (Print)}, doi = {10.1002/nag.530}, pages = {1357 -- 1367}, year = {2006}, language = {en} } @article{ScheerSnaithWolfetal.2013, author = {Scheer, Nico and Snaith, Mike and Wolf, C. Roland and Seibler, Jost}, title = {Generation and utility of genetically humanized mouse models}, series = {Drug Discovery Today}, volume = {Vol 18}, journal = {Drug Discovery Today}, number = {23-24}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1359-6446}, doi = {10.1016/j.drudis.2013.07.007}, pages = {1200 -- 1211}, year = {2013}, language = {en} } @article{BouwmanGuldenHeijdenetal.2013, author = {Bouwman, Peter and Gulden, Hanneke van der and Heijden, Ingrid van der and Drost, Rinske and Klijn, Christiaan N. and Prasetyanti, Pramudita and Pieterse, Mark and Wientjens, Ellen and Seibler, Jost and Hogervorst, Frank B. L. and Jonkers, Jos}, title = {A High-Throughput Functional Complementation Assay for Classification of BRCA1 Missense Variants}, series = {Cancer Discovery}, journal = {Cancer Discovery}, number = {3}, issn = {2159-8290}, doi = {10.1158/2159-8290.CD-13-0094}, pages = {1142 -- 1152}, year = {2013}, language = {en} } @article{MichalakNacerddinePietersenetal.2013, author = {Michalak, Ewa Malgorzata and Nacerddine, Karim and Pietersen, Alexandra and Beuger, Vincent and Pawlitzky, Inka and Cornelissen-Steijger, Paulien and Wientjens, Ellen and Tanger, Ellen and Seibler, Jost and Lohuizen, Maarten van and Jonkers, Jos}, title = {Polycomb group gene Ezh2 regulates mammary gland morphogenesis and maintains the luminal progenitor pool}, series = {Stem Cells}, volume = {Vol 31}, journal = {Stem Cells}, number = {9}, publisher = {Oxford University Press}, address = {Oxford}, issn = {1549-4918}, doi = {10.1002/stem.1437}, pages = {1910 -- 1920}, year = {2013}, language = {en} } @article{GebeshuberKornauthDongetal.2013, author = {Gebeshuber, Christoph A. and Kornauth, Christoph and Dong, Lihua and Sierig, Ralph and Seibler, Jost and Reiss, Martina and Tauber, Stefanie and Bilban, Martin and Wang, Shijun and Kain, Renate and B{\"o}hmig, Georg A. and Moeller, Marcus J. and Gr{\"o}ne, Hermann-Josef and Englert, Christoph and Martinez, Javier and Kerjaschki, Dontscho}, title = {Focal segmental glomerulosclerosis is induced by microRNA-193a and its downregulation of WT1}, series = {Nature Medicine}, volume = {19}, journal = {Nature Medicine}, number = {4}, issn = {1078-8956}, doi = {10.1038/nm.3142}, pages = {481 -- 487}, year = {2013}, language = {en} } @article{KornfeldBaitzelKoenneretal.2013, author = {Kornfeld, Jan-Wilhelm and Baitzel, Catherina and K{\"o}nner, A. Christine and Nicholls, Hayley T. and Vogt, Merly C. and Herrmanns, Karolin and Scheja, Ludger and Haumaitre, C{\´e}cile and Wolf, Anna M. and Knippschild, Uwe and Seibler, Jost and Cereghini, Silvia and Heeren, Joerg and Stoffel, Markus and Br{\"u}ning, Jens C.}, title = {Obesity-induced overexpression of miR-802 impairs glucose metabolism through silencing of Hnf1b}, series = {Nature}, volume = {494}, journal = {Nature}, number = {7435}, publisher = {Springer Nature}, address = {Cham}, isbn = {0028-0836}, doi = {10.1038/nature11793}, pages = {111 -- 115}, year = {2013}, language = {en} } @article{Wollert2008, author = {Wollert, J{\"o}rg}, title = {Bluetooth: Funk f{\"u}r alle(s)? : 10 Jahre Bluetooth: ein Technologie-{\"U}ber- und -Ausblick}, series = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, journal = {Elektronik : Fachmedium f{\"u}r industrielle Anwender und Entwickler ; Sonderh. Wireless : Fachmedium f{\"u}r Entwicklungen funkbasierter Systeme}, number = {Okt.}, pages = {36 -- 43}, year = {2008}, language = {de} } @article{OberlaenderKirchnerKeusgenetal.2015, author = {Oberl{\"a}nder, Jan and Kirchner, Patrick and Keusgen, Michael and Sch{\"o}ning, Michael Josef}, title = {Strategies in developing thin-film sensors for monitoring aseptic food processes : Theoretical considerations and investigations of passivation materials}, series = {Electrochimica Acta}, volume = {183}, journal = {Electrochimica Acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2015.06.126}, pages = {130 -- 136}, year = {2015}, abstract = {The sterilization of packages in aseptic food processes is highly significant to maintain a consumer-safe product with extended shelf-life. Today, the sterilization of food packages is predominantly accomplished by gaseous hydrogen peroxide (H2O2) in combination with heat. In order to monitor this sterilization process, calorimetric gas sensors as differential set-up of two platinum temperature sensors representing a catalytically active (additionally deposition of MnO2) and a passive segment have been recently developed. The temperature rise of the exothermic decomposition serves as an indicator of the present H2O2 concentration. In the present work, a theoretical approach considering the sensor's thermochemistry and physical transport phenomena was formulated to evaluate the temperature rise based on the energy content of gaseous H2O2. In a further part of this work, three polymers have been analyzed with respect to their application as passivation materials. The examined polymers are photoresist SU-8, perfluoroalkoxy (PFA) and fluorinated ethylene propylene (FEP). Thermal analyses by means of differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) have been conducted to determine the operation limits of the polymers. The overall chemical resistance and stability of the polymers against the harsh environmental conditions during the sterilization process have been examined by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR).}, language = {en} } @article{KuperjansWeitzel2015, author = {Kuperjans, Isabel and Weitzel, J.}, title = {Energiedesign 2020 : Sichere Strom- und W{\"a}rmeversorgung f{\"u}r die Industrie}, series = {TAB: das Fachmedium der TGA-Branche}, journal = {TAB: das Fachmedium der TGA-Branche}, number = {3}, publisher = {Bauverlag}, address = {G{\"u}tersloh}, issn = {0341-2032}, pages = {105 -- 107}, year = {2015}, language = {de} } @article{HoevelerJanserBindewaldetal.2015, author = {Hoeveler, Bastian and Janser, Frank and Bindewald, Thorsten and Gebhardt, Andreas}, title = {Entwurf, Fertigung und Untersuchung eines Windkanalmodells eines innovativen, senkrechtstartenden Kleinflugzeuges}, series = {RTejournal - Forum f{\"u}r Rapid Technologie}, journal = {RTejournal - Forum f{\"u}r Rapid Technologie}, number = {12}, publisher = {Fachhochschule Aachen}, address = {Aachen}, issn = {1614-0923}, url = {http://nbn-resolving.de/urn:nbn:de:0009-2-42921}, pages = {1 -- 5}, year = {2015}, language = {de} } @article{Wollert2015, author = {Wollert, J{\"o}rg}, title = {IO-Link - f{\"u}r smarte Sensoren}, series = {elektroniknet.de}, journal = {elektroniknet.de}, year = {2015}, language = {de} } @article{Wollert2015, author = {Wollert, J{\"o}rg}, title = {Echtzeit-Ethernet : Protokoll-Reigen}, series = {elektroniknet.de}, journal = {elektroniknet.de}, year = {2015}, language = {de} } @article{MiyamatoSakakitaWagneretal.2015, author = {Miyamato, Ko-ichiro and Sakakita, Sakura and Wagner, Torsten and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Application of chemical imaging sensor to in-situ pH imaging in the vicinity of a corroding metal surface}, series = {Electrochimica Acta}, volume = {183}, journal = {Electrochimica Acta}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0013-4686}, doi = {10.1016/j.electacta.2015.07.184}, pages = {137 -- 142}, year = {2015}, abstract = {The chemical imaging sensor was applied to in-situ pH imaging of the solution in the vicinity of a corroding surface of stainless steel under potentiostatic polarization. A test piece of polished stainless steel was placed on the sensing surface leaving a narrow gap filled with artificial seawater and the stainless steel was corroded under polarization. The pH images obtained during polarization showed correspondence between the region of lower pH and the site of corrosion. It was also found that the pH value in the gap became as low as 2 by polarization, which triggered corrosion.}, language = {en} } @article{HamadBilattoAdlyetal.2016, author = {Hamad, E. M. and Bilatto, S. E. R. and Adly, N. Y. and Correa, D. S. and Wolfrum, B. and Sch{\"o}ning, Michael Josef and Offenh{\"a}usser, A. and Yakushenko, A.}, title = {Inkjet printing of UV-curable adhesive and dielectric inks for microfluidic devices}, series = {Lab on a Chip}, volume = {16}, journal = {Lab on a Chip}, number = {1}, publisher = {Royal Society of Chemistry}, address = {Cambridge}, issn = {1473-0189}, doi = {10.1039/C5LC01195G}, pages = {70 -- 74}, year = {2016}, abstract = {Bonding of polymer-based microfluidics to polymer substrates still poses a challenge for Lab-On-a-Chip applications. Especially, when sensing elements are incorporated, patterned deposition of adhesives with curing at ambient conditions is required. Here, we demonstrate a fabrication method for fully printed microfluidic systems with sensing elements using inkjet and stereolithographic 3D-printing.}, language = {en} } @article{HauserKotliarTholenetal.2015, author = {Hauser, C. and Kotliar, Konstantin and Tholen, S. and Hasenau, A. and Suttmann, Y. and Renders, L. and Heemann, U. and Baumann, M. and Schmaderer, C.}, title = {Dynamische retinale Gef{\"a}ßreaktion bei H{\"a}modialysepatienten}, series = {Nieren- und Hochdruckkrankheiten}, volume = {44}, journal = {Nieren- und Hochdruckkrankheiten}, number = {11}, publisher = {Dustri-Verlag}, address = {Oberhaching}, issn = {0300-5224}, doi = {10.5414/NHX01743a}, pages = {480 -- 480}, year = {2015}, language = {de} } @article{Gebhardt2015, author = {Gebhardt, Andreas}, title = {3D-Druck ist {\"u}berall. Modische Accessoires, Kleidung, Medikamente, Nahrungsmittel, Autos, H{\"a}user, Waffen, B{\"u}sten, Musikinstrumente - alles geht}, series = {Kunststoffe}, volume = {105}, journal = {Kunststoffe}, number = {10}, publisher = {Hanser}, address = {M{\"u}nchen}, issn = {0023-5563}, pages = {62 -- 70}, year = {2015}, language = {de} } @article{FischerSelverGezeretal.2015, author = {Fischer, Felix and Selver, M. Alper and Gezer, Sinem and Dicle, Oguz and Hillen, Walter}, title = {Systematic Parameterization, Storage, and Representation of Volumetric DICOM Data}, series = {Journal of Medical and Biological Engineering}, volume = {35}, journal = {Journal of Medical and Biological Engineering}, number = {6}, publisher = {Springer}, address = {Berlin}, issn = {2199-4757}, doi = {10.1007/s40846-015-0097-5}, pages = {709 -- 723}, year = {2015}, language = {en} } @article{FredebeulKrein2015, author = {Fredebeul-Krein, Markus}, title = {Volkswirtschaftslehre : Die Klausur}, series = {Das Wirtschaftsstudium : wisu ; Zeitschrift f{\"u}r Ausbildung, Pr{\"u}fung, Berufseinstieg und Fortbildung}, volume = {45}, journal = {Das Wirtschaftsstudium : wisu ; Zeitschrift f{\"u}r Ausbildung, Pr{\"u}fung, Berufseinstieg und Fortbildung}, number = {11}, publisher = {Lange}, address = {D{\"u}sseldorf}, issn = {0340-3084}, pages = {1253 -- 1255}, year = {2015}, language = {de} } @article{FredebeulKrein2015, author = {Fredebeul-Krein, Markus}, title = {Wettbewerbspolitische Leitbilder}, series = {Das Wirtschaftsstudium : wisu ; Zeitschrift f{\"u}r Ausbildung, Examen, Berufseinstieg und Fortbildung}, volume = {45}, journal = {Das Wirtschaftsstudium : wisu ; Zeitschrift f{\"u}r Ausbildung, Examen, Berufseinstieg und Fortbildung}, number = {4}, publisher = {Lange}, address = {D{\"u}sseldorf}, issn = {0340-3084}, pages = {483 -- 486}, year = {2015}, language = {de} } @article{FredebeulKrein2016, author = {Fredebeul-Krein, Markus}, title = {Warum staatliche Kaufpr{\"a}mien f{\"u}r Elektroautos abzulehnen sind}, series = {Wirtschaftliche Freiheit : das ordnungspolitische Journal}, volume = {2016}, journal = {Wirtschaftliche Freiheit : das ordnungspolitische Journal}, number = {4}, publisher = {Alexander B. Brunner}, address = {W{\"u}rzburg}, year = {2016}, abstract = {Im Jahr 2015 wurden in Deutschland {\"u}ber drei Millionen Benzinautos und lediglich 12.363 Elektroautos neu zugelassen. Das urspr{\"u}nglich von der Bundesregierung vorgegebene Ziel, dass bis 2020 eine Million E-Autos auf deutschen Straßen fahren (und bis 2030 sechs Millionen), r{\"u}ckt damit in immer weitere Ferne. Um das Ziel dennoch zu erreichen, plant die Bundesregierung nun eine staatli­che Pr{\"a}mie f{\"u}r den Kauf von Elektroautos: Umwelt-, Verkehrs- und Wirtschaftsministerium haben gemeinsam ein Konzept entworfen, dem zufolge private K{\"a}ufer zuk{\"u}nftig einen Zuschuss von 5.000 Euro beim Erwerb eines Elektroautos bekommen sollen. 40 Prozent dieses Zuschusses soll von den Autoherstellern getragen werden. Das Programm, das weitere ausgabenwirksame {\"o}ffentli­che Maßnahmen vorsieht, w{\"u}rde Kosten in Milliarden­h{\"o}he verursachen. Die beabsichtigte Subventionierung wirft die Frage auf, ob diese wirtschaftlich sinnvoll sind.}, language = {de} } @article{BayonValeroGarciaBartualetal.2016, author = {Bayon, Arnau and Valero, Daniel and Garcia-Bartual, Rafael and Vall{\´e}s-Mor{\´a}n, Francisco Jos{\´e} and L{\´o}pez-Jim{\´e}nez, P. Amparo}, title = {Performance assessment of OpenFOAM and FLOW-3D in the numerical modeling of a low Reynolds number hydraulic jump}, series = {Environmental Modelling \& Software}, volume = {80}, journal = {Environmental Modelling \& Software}, publisher = {Elsevier}, address = {Amsterdam}, isbn = {1364-8152}, doi = {10.1016/j.envsoft.2016.02.018}, pages = {322 -- 335}, year = {2016}, abstract = {A comparative performance analysis of the CFD platforms OpenFOAM and FLOW-3D is presented, focusing on a 3D swirling turbulent flow: a steady hydraulic jump at low Reynolds number. Turbulence is treated using RANS approach RNG k-ε. A Volume Of Fluid (VOF) method is used to track the air-water interface, consequently aeration is modeled using an Eulerian-Eulerian approach. Structured meshes of cubic elements are used to discretize the channel geometry. The numerical model accuracy is assessed comparing representative hydraulic jump variables (sequent depth ratio, roller length, mean velocity profiles, velocity decay or free surface profile) to experimental data. The model results are also compared to previous studies to broaden the result validation. Both codes reproduced the phenomenon under study concurring with experimental data, although special care must be taken when swirling flows occur. Both models can be used to reproduce the hydraulic performance of energy dissipation structures at low Reynolds numbers.}, language = {en} } @article{MiyamotoYuIsodaetal.2016, author = {Miyamoto, Ko-ichiro and Yu, Bing and Isoda, Hiroko and Wagner, Torsten and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo}, title = {Visualization of the recovery process of defects in a cultured cell layer by chemical imaging sensor}, series = {Sensors and Actuators B: Chemical}, volume = {236}, journal = {Sensors and Actuators B: Chemical}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0925-4005}, doi = {10.1016/j.snb.2016.04.018}, pages = {965 -- 969}, year = {2016}, abstract = {The chemical imaging sensor is a field-effect sensor which is able to visualize both the distribution of ions (in LAPS mode) and the distribution of impedance (in SPIM mode) in the sample. In this study, a novel cell assay is proposed, in which the chemical imaging sensor operated in SPIM mode is applied to monitor the recovery of defects in a cell layer brought into proximity of the sensing surface. A reduced impedance at a defect formed artificially in a cell layer was successfully visualized in a photocurrent image. The cell layer was cultured over two weeks, during which the temporal change of the photocurrent distribution corresponding to the recovery of the defect was observed.}, language = {de} } @article{DantismTakenagaWagneretal.2016, author = {Dantism, Shahriar and Takenaga, Shoko and Wagner, Patrick and Wagner, Torsten and Sch{\"o}ning, Michael Josef}, title = {Determination of the extracellular acidification of Escherichia coli K12 with a multi-​chamber-​based LAPS system}, series = {Physica status solidi (a)}, volume = {213}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300}, doi = {10.1002/pssa.201533043}, pages = {1479 -- 1485}, year = {2016}, abstract = {On-line monitoring of the metabolic activity of microorganisms involved in intermediate stages of biogas production plays an important role to avoid undesirable "down times" during the biogas production. In order to control this process, an on-chip differential measuring system based on the light-addressable potentiometric sensor (LAPS) principle combined with a 3D-printed multi-chamber structure has been realized. As a test microorganism, Escherichia coli K12 (E. coli K12) were used for cell-based measurements. Multi-chamber structures were developed to determine the metabolic activity of E. coli K12 in suspension for a different number of cells, responding to the addition of a constant or variable amount of glucose concentrations, enabling differential and simultaneous measurements.}, language = {en} } @article{Wollert2016, author = {Wollert, J{\"o}rg}, title = {Rapid Application Development}, series = {Design \& Elektronik}, journal = {Design \& Elektronik}, number = {4}, publisher = {WEKA-Fachmedien}, address = {M{\"u}nchen}, issn = {0933-8667}, pages = {8 -- 11}, year = {2016}, abstract = {Das IoT ist ohne eingebettete Systeme undenkbar. Erst kleine und kleinste Mikrocontroller mit intelligenten Kommunikationsschnittstellen und Anbindung ans Internet erm{\"o}glichen sinnvolles und fl{\"a}chendeckendes Einsammeln von Daten. Doch wie kompliziert ist der Einstieg in die Embedded-Welt? Dieser Artikel gibt Einblick, wie die »Arduino-Plattform« die Einstiegsh{\"u}rden f{\"u}r eingebettete Systeme dramatisch reduzieren kann.}, language = {de} } @article{Wollert2016, author = {Wollert, J{\"o}rg}, title = {Industrial Internet of Things : Seifenblase oder Revolution}, series = {Elektronik}, journal = {Elektronik}, number = {7}, publisher = {WEKA-Fachmedien}, address = {M{\"u}nchen}, issn = {0013-5658}, pages = {39 -- 45}, year = {2016}, language = {de} } @article{BreuerRaueStrobeletal.2016, author = {Breuer, Lars and Raue, Markus and Strobel, M. and Mang, Thomas and Sch{\"o}ning, Michael Josef and Thoelen, R. and Wagner, Torsten}, title = {Hydrogels with incorporated graphene oxide as light-addressable actuator materials for cell culture environments in lab-on-chip systems}, series = {Physica status solidi (a)}, volume = {213}, journal = {Physica status solidi (a)}, number = {6}, publisher = {Wiley-VCH}, address = {Weinheim}, issn = {1862-6300}, doi = {10.1002/pssa.201533056}, pages = {1520 -- 1525}, year = {2016}, abstract = {Abstractauthoren Graphene oxide (GO) nanoparticles were incorporated in temperature-sensitive Poly(N-isopropylacrylamide) (PNIPAAm) hydrogels. The nanoparticles increase the light absorption and convert light energy into heat efficiently. Thus, the hydrogels with GO can be stimulated spatially resolved by illumination as it was demonstrated by IR thermography. The temporal progression of the temperature maximum was detected for different concentrations of GO within the polymer network. Furthermore, the compatibility of PNIPAAm hydrogels with GO and cell cultures was investigated. For this purpose, culture medium was incubated with hydrogels containing GO and the viability and morphology of chinese hamster ovary (CHO) cells was examined after several days of culturing in presence of this medium.}, language = {en} } @article{PookhalilAmoabedinyTabeshetal.2016, author = {Pookhalil, Ali and Amoabediny, Ghassem and Tabesh, Hadi and Behbahani, Mehdi and Mottaghy, Khosrow}, title = {A new approach for semiempirical modeling of mechanical blood trauma}, series = {The international journal of artificial organs}, volume = {39}, journal = {The international journal of artificial organs}, number = {4}, publisher = {Sage}, address = {London}, issn = {1724-6040}, doi = {10.5301/ijao.5000474}, pages = {171 -- 177}, year = {2016}, abstract = {Purpose Two semi-empirical models were recently published, both making use of existing literature data, but each taking into account different physical phenomena that trigger hemolysis. In the first model, hemoglobin (Hb) release is described as a permeation procedure across the membrane, assuming a shear stress-dependent process (sublethal model). The second model only accounts for hemoglobin release that is caused by cell membrane breakdown, which occurs when red blood cells (RBC) undergo mechanically induced shearing for a period longer than the threshold time (nonuniform threshold model). In this paper, we introduce a model that considers the hemolysis generated by both these possible phenomena. Methods Since hemolysis can possibly be caused by permeation of hemoglobin through the RBC functional membrane as well as by release of hemoglobin from RBC membrane breakdown, our proposed model combines both these models. An experimental setup consisting of a Couette device was utilized for validation of our proposed model. Results A comparison is presented between the damage index (DI) predicted by the proposed model vs. the sublethal model vs. the nonthreshold model and experimental datasets. This comparison covers a wide range of shear stress for both human and porcine blood. An appropriate agreement between the measured DI and the DI predicted by the present model was obtained. Conclusions The semiempirical hemolysis model introduced in this paper aims for significantly enhanced conformity with experimental data. Two phenomenological outcomes become possible with the proposed approach: an estimation of the average time after which cell membrane breakdown occurs under the applied conditions, and a prediction of the ratio between the phenomena involved in hemolysis.}, language = {en} } @article{Lind2016, author = {Lind, Thorsten Patric}, title = {Insolvenzanfechtung: Keine Gl{\"a}ubigerbenachteiligung bei Abl{\"o}sezahlung gegen Forderungsverzicht}, series = {Der Betrieb}, volume = {69}, journal = {Der Betrieb}, number = {17}, publisher = {Fachmedien Otto Schmidt}, address = {D{\"u}sseldorf}, issn = {0005-9935}, pages = {999 -- 1001}, year = {2016}, abstract = {Schwerpunkt einer vorinsolvenzlichen Sanierung ist i.d.R., dass einzelne Gl{\"a}ubiger gegen Teilzahlungen auf ihre Forderungen verzichten und somit dem Unternehmen den notwendigen finanziellen Freiraum f{\"u}r einen Turnaround geben. Die Motivation der Gl{\"a}ubiger f{\"u}r einen Verzicht ist dabei auch die {\"U}berlegung, dass eine quotale Befriedigung im Rahmen eines Insolvenzverfahrens oft wesentlich geringer ausfallen w{\"u}rde als die durch den Vergleich realisierte Teilzahlung. Das Risiko, im Falle der Insolvenz eine Teilzahlung im Rahmen der Insolvenzanfechtung aber wieder zur{\"u}ckgew{\"a}hren zu m{\"u}ssen, macht einen Forderungsverzicht weniger attraktiv. Mit Urteil vom 28.01.2016 hat der BGH nun entschieden, dass eine Insolvenzanfechtung mangels Gl{\"a}ubigerbenachteiligung ausscheidet, wenn der in der Teilzahlung liegende Verm{\"o}gensverlust durch den damit verbundenen Verzicht auf die Restforderung voll ausgeglichen wird. Im Folgenden wird untersucht, ob dieses Urteil als Blaupause f{\"u}r eine anfechtungsfeste Restrukturierung einzelner Verbindlichkeiten dienen kann.}, language = {de} } @article{BertzSchoeningMolinnusetal.2024, author = {Bertz, Morten and Sch{\"o}ning, Michael Josef and Molinnus, Denise and Homma, Takayuki}, title = {Influence of temperature, light, and H₂O₂ concentration on microbial spore inactivation: in-situ Raman spectroscopy combined with optical trapping}, series = {Physica status solidi (a) applications and materials science}, journal = {Physica status solidi (a) applications and materials science}, number = {Early View}, publisher = {Wiley-VCH}, address = {Berlin}, issn = {1862-6319 (Online)}, doi = {10.1002/pssa.202300866}, pages = {8 Seiten}, year = {2024}, abstract = {To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30\% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.}, language = {en} } @article{SchoenrockMuckeltHastermannetal.2024, author = {Schoenrock, Britt and Muckelt, Paul E. and Hastermann, Maria and Albracht, Kirsten and MacGregor, Robert and Martin, David and Gunga, Hans-Christian and Salanova, Michele and Stokes, Maria J. and Warner, Martin B. and Blottner, Dieter}, title = {Muscle stiffness indicating mission crew health in space}, series = {Scientific Reports}, volume = {14}, journal = {Scientific Reports}, number = {Article number: 4196}, publisher = {Springer Nature}, address = {London}, issn = {2045-2322}, doi = {10.1038/s41598-024-54759-6}, pages = {13 Seiten}, year = {2024}, abstract = {Muscle function is compromised by gravitational unloading in space affecting overall musculoskeletal health. Astronauts perform daily exercise programmes to mitigate these effects but knowing which muscles to target would optimise effectiveness. Accurate inflight assessment to inform exercise programmes is critical due to lack of technologies suitable for spaceflight. Changes in mechanical properties indicate muscle health status and can be measured rapidly and non-invasively using novel technology. A hand-held MyotonPRO device enabled monitoring of muscle health for the first time in spaceflight (> 180 days). Greater/maintained stiffness indicated countermeasures were effective. Tissue stiffness was preserved in the majority of muscles (neck, shoulder, back, thigh) but Tibialis Anterior (foot lever muscle) stiffness decreased inflight vs. preflight (p < 0.0001; mean difference 149 N/m) in all 12 crewmembers. The calf muscles showed opposing effects, Gastrocnemius increasing in stiffness Soleus decreasing. Selective stiffness decrements indicate lack of preservation despite daily inflight countermeasures. This calls for more targeted exercises for lower leg muscles with vital roles as ankle joint stabilizers and in gait. Muscle stiffness is a digital biomarker for risk monitoring during future planetary explorations (Moon, Mars), for healthcare management in challenging environments or clinical disorders in people on Earth, to enable effective tailored exercise programmes.}, language = {en} } @article{PolenKraemerBongaertsetal.2005, author = {Polen, T. and Kr{\"a}mer, Marco and Bongaerts, Johannes and Wubbolts, Marcel and Wendisch, V. F.}, title = {The global gene expression response of Escherichia coli to L-phenylalanine}, series = {Journal of biotechnology}, volume = {Vol. 115}, journal = {Journal of biotechnology}, number = {Iss. 3}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, pages = {221 -- 237}, year = {2005}, language = {en} } @article{BongaertsKraemerMuelleretal.2001, author = {Bongaerts, Johannes and Kr{\"a}mer, Marco and M{\"u}ller, Ulrike and Raeven, Leon and Wubbolts, Marcel}, title = {Metabolic engineering for microbial production of aromatic amino acids and derived compounds}, series = {Metabolic engineering}, volume = {Vol. 3}, journal = {Metabolic engineering}, number = {Iss. 4}, issn = {1096-7184 (E-Journal); 1096-7176 (Print)}, pages = {289 -- 300}, year = {2001}, language = {en} } @article{AbbasBalcBremenetal.2022, author = {Abbas, Karim and Balc, Nicolae and Bremen, Sebastian and Skupin, Marco}, title = {Crystallization and aging behavior of polyetheretherketone PEEK within rapid tooling and rubber molding}, series = {Journal of Manufacturing and Materials Processing}, volume = {6}, journal = {Journal of Manufacturing and Materials Processing}, number = {5}, publisher = {MDPI}, address = {Basel}, issn = {2504-4494}, doi = {10.3390/jmmp6050093}, pages = {1 -- 12}, year = {2022}, abstract = {In times of short product life cycles, additive manufacturing and rapid tooling are important methods to make tool development and manufacturing more efficient. High-performance polymers are the key to mold production for prototypes and small series. However, the high temperatures during vulcanization injection molding cause thermal aging and can impair service life. The extent to which the thermal stress over the entire process chain stresses the material and whether it leads to irreversible material aging is evaluated. To this end, a mold made of PEEK is fabricated using fused filament fabrication and examined for its potential application. The mold is heated to 200 ◦C, filled with rubber, and cured. A differential scanning calorimetry analysis of each process step illustrates the crystallization behavior and first indicates the material resistance. It shows distinct cold crystallization regions at a build chamber temperature of 90 ◦C. At an ambient temperature above Tg, crystallization of 30\% is achieved, and cold crystallization no longer occurs. Additional tensile tests show a decrease in tensile strength after ten days of thermal aging. The steady decrease in recrystallization temperature indicates degradation of the additives. However, the tensile tests reveal steady embrittlement of the material due to increasing crosslinking.}, language = {en} } @article{Bung2024, author = {Bung, Daniel Bernhard}, title = {Kamerabasierte Fließtiefen- und Geschwindigkeitsmessungen}, series = {Wasserwirtschaft}, volume = {114}, journal = {Wasserwirtschaft}, number = {4}, publisher = {Springer Vieweg}, address = {Wiesbaden}, issn = {0043-0978}, pages = {47 -- 53}, year = {2024}, abstract = {In der wasserbaulichen Forschung werden neben klassischen Messinstrumenten zunehmend kamerabasierte Verfahren genutzt. Diese erlauben neben der Bestimmung von Fließgeschwindigkeiten auch die Detektion der freien Wasseroberfl{\"a}che oder zeitliche Vermessung von Kolken. Durch die hohen r{\"a}umlichen und zeitlichen Aufl{\"o}sungen, welche neueste Kamerasensoren liefern, k{\"o}nnen neue Erkenntnisse in turbulenten, komplexen Str{\"o}mungen gewonnen werden. Auch in der Praxis k{\"o}nnen diese Verfahren mit geringem Aufwand wichtige Daten liefern.}, language = {de} } @article{FunkeBeckmann2022, author = {Funke, Harald and Beckmann, Nils}, title = {Flexible fuel operation of a Dry-Low-NOx Micromix Combustor with Variable Hydrogen Methane Mixture}, series = {International Journal of Gas Turbine, Propulsion and Power Systems}, volume = {13}, journal = {International Journal of Gas Turbine, Propulsion and Power Systems}, number = {2}, issn = {1882-5079}, pages = {1 -- 7}, year = {2022}, abstract = {The role of hydrogen (H2) as a carbon-free energy carrier is discussed since decades for reducing greenhouse gas emissions. As bridge technology towards a hydrogen-based energy supply, fuel mixtures of natural gas or methane (CH4) and hydrogen are possible. The paper presents the first test results of a low-emission Micromix combustor designed for flexible-fuel operation with variable H2/CH4 mixtures. The numerical and experimental approach for considering variable fuel mixtures instead of recently investigated pure hydrogen is described. In the experimental studies, a first generation FuelFlex Micromix combustor geometry is tested at atmospheric pressure at gas turbine operating conditions corresponding to part- and full-load. The H2/CH4 fuel mixture composition is varied between 57 and 100 vol.\% hydrogen content. Despite the challenges flexible-fuel operation poses onto the design of a combustion system, the evaluated FuelFlex Micromix prototype shows a significant low NOx performance}, language = {en} } @article{EdipSesovButenwegetal.2018, author = {Edip, K. and Sesov, V. and Butenweg, Christoph and Bojadjieva, J.}, title = {Development of coupled numerical model for simulation of multiphase soil}, series = {Computers and Geotechnics}, volume = {96}, journal = {Computers and Geotechnics}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0266-352X}, doi = {10.1016/j.compgeo.2017.08.016}, pages = {118 -- 131}, year = {2018}, abstract = {In this paper, a coupled multiphase model considering both non-linearities of water retention curves and solid state modeling is proposed. The solid displacements and the pressures of both water and air phases are unknowns of the proposed model. The finite element method is used to solve the governing differential equations. The proposed method is demonstrated through simulation of seepage test and partially consolidation problem. Then, implementation of the model is done by using hypoplasticity for the solid phase and analyzing the fully saturated triaxial experiments. In integration of the constitutive law error controlling is improved and comparisons done accordingly. In this work, the advantages and limitations of the numerical model are discussed.}, language = {en} } @article{ZhangValeroBungetal.2018, author = {Zhang, G. and Valero, Daniel and Bung, Daniel Bernhard and Chanson, H.}, title = {On the estimation of free-surface turbulence using ultrasonic sensors}, series = {Flow Measurement and Instrumentation}, volume = {60}, journal = {Flow Measurement and Instrumentation}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0955-5986}, doi = {10.1016/j.flowmeasinst.2018.02.009}, pages = {171 -- 184}, year = {2018}, abstract = {Accurate determination of free-surface dynamics has attracted much research attention during the past decade and has important applications in many environmental and water related areas. In this study, the free-surface dynamics in several turbulent flows commonly found in nature were investigated using a synchronised setup consisting of an ultrasonic sensor and a high-speed video camera. Basic sensor capabilities were examined in dry conditions to allow for a better characterisation of the present sensor model. The ultrasonic sensor was found to adequately reproduce free-surface dynamics up to the second order, especially in two-dimensional scenarios with the most energetic modes in the low frequency range. The sensor frequency response was satisfactory in the sub-20 Hz band, and its signal quality may be further improved by low-pass filtering prior to digitisation. The application of the USS to characterise entrapped air in high-velocity flows is also discussed.}, language = {en} } @article{BongaertsEsserLorbachetal.2011, author = {Bongaerts, Johannes and Esser, Simon and Lorbach, Volker and Al-Momani, L{\´o}ay and M{\"u}ller, Michael A. and Franke, Dirk and Grondal, Christoph and Kurutsch, Anja and Bujnicki, Robert and Takors, Ralf and Raeven, Leon and Wubbolts, Marcel and Bovenberg, Roel and Nieger, Martin and Sch{\"u}rmann, Melanie and Trachtmann, Natalie and Kozak, Stefan and Sprenger, Georg A. and M{\"u}ller, Michael}, title = {Diversity-oriented production of metabolites derived from chorismate and their use in organic synthesis}, series = {Angewandte Chemie International Edition}, volume = {Vol. 50}, journal = {Angewandte Chemie International Edition}, number = {Iss. 34}, publisher = {Wiley}, address = {Weinheim}, issn = {1521-3773 (E-Journal); 0570-0833 (Print); 1433-7851 (Print)}, pages = {7781 -- 7786}, year = {2011}, language = {en} } @article{MarinkovicButenweg2022, author = {Marinkovic, Marko and Butenweg, Christoph}, title = {Numerical analysis of the in-plane behaviour of decoupled masonry infilled RC frames}, series = {Engineering Structures}, volume = {272}, journal = {Engineering Structures}, number = {1}, publisher = {Elsevier}, address = {Amsterdam}, issn = {0141-0296}, doi = {10.1016/j.engstruct.2022.114959}, pages = {18 Seiten}, year = {2022}, abstract = {Damage of reinforced concrete (RC) frames with masonry infill walls has been observed after many earthquakes. Brittle behaviour of the masonry infills in combination with the ductile behaviour of the RC frames makes infill walls prone to damage during earthquakes. Interstory deformations lead to an interaction between the infill and the RC frame, which affects the structural response. The result of this interaction is significant damage to the infill wall and sometimes to the surrounding structural system too. In most design codes, infill walls are considered as non-structural elements and neglected in the design process, because taking into account the infills and considering the interaction between frame and infill in software packages can be complicated and impractical. A good way to avoid negative aspects arising from this behavior is to ensure no or low-interaction of the frame and infill wall, for instance by decoupling the infill from the frame. This paper presents the numerical study performed to investigate new connection system called INODIS (Innovative Decoupled Infill System) for decoupling infill walls from surrounding frame with the aim to postpone infill activation to high interstory drifts thus reducing infill/frame interaction and minimizing damage to both infills and frames. The experimental results are first used for calibration and validation of the numerical model, which is then employed for investigating the influence of the material parameters as well as infill's and frame's geometry on the in-plane behaviour of the infilled frames with the INODIS system. For all the investigated situations, simulation results show significant improvements in behaviour for decoupled infilled RC frames in comparison to the traditionally infilled frames.}, language = {en} } @article{BungErpicumTullis2020, author = {Bung, Daniel Bernhard and Erpicum, S{\´e}bastien and Tullis, Blanke P.}, title = {Advances in hydraulic structures engineering}, series = {Journal of Hydraulic Engineering}, volume = {147}, journal = {Journal of Hydraulic Engineering}, number = {1}, publisher = {ASCE}, address = {Reston, Va.}, issn = {0733-9429 (Druckausgabe)}, doi = {10.1061/(ASCE)HY.1943-7900.0001851}, pages = {1 Seite}, year = {2020}, language = {en} }