@article{RibitschKarlBirnerGruenbergeretal.2010, author = {Ribitsch, Doris and Karl, Wolfgang and Birner-Gruenberger, Ruth and Gruber, Karl and Eiteljoerg, Inge and Remler, Peter and Wieland, Susanne and Siegert, Petra and Maurer, Karl-Heinz and Schwab, Helmut}, title = {C-terminal truncation of a metagenome-derived detergent protease for effective expression in E. coli}, series = {Journal of biotechnology}, volume = {150}, journal = {Journal of biotechnology}, number = {3}, publisher = {Elsevier}, address = {Amsterdam}, issn = {1873-4863 (E-Journal); 0168-1656 (Print)}, doi = {10.1016/j.jbiotec.2010.09.947}, pages = {408 -- 416}, year = {2010}, abstract = {Recently, a new alkaline protease named HP70 showing highest homology to extracellular serine proteases of Stenotrophomonas maltophilia and Xanthomonas campestris was found in the course of a metagenome screening for detergent proteases (Niehaus et al., submitted for publication). Attempts to efficiently express the enzyme in common expression hosts had failed. This study reports on the realization of overexpression in Escherichia coli after structural modification of HP70. Modelling of HP70 resulted in a two-domain structure, comprising the catalytic domain and a C-terminal domain which includes about 100 amino acids. On the basis of the modelled structure the enzyme was truncated by deletion of most of the C-terminal domain yielding HP70-C477. This structural modification allowed effective expression of active enzyme using E. coli BL21-Gold as the host. Specific activity of HP70-C477 determined with suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide as the substrate was 30 ± 5 U/mg compared to 8 ± 1 U/mg of the native enzyme. HP70-C477 was most active at 40 °C and pH 7-11; these conditions are prerequisite for a potential application as detergent enzyme. Determination of kinetic parameters at 40 °C and pH = 9.5 resulted in KM = 0.23 ± 0.01 mM and kcat = 167.5 ± 3.6 s⁻¹. MS-analysis of peptide fragments obtained from incubation of HP70 and HP70-C477 with insulin B indicated that the C-terminal domain influences the cleavage preferences of the enzyme. Washing experiments confirmed the high potential of HP70-C477 as detergent protease.}, language = {en} } @inproceedings{GoettenFingerBraunetal.2019, author = {G{\"o}tten, Falk and Finger, Felix and Braun, Carsten and Havermann, Marc and Bil, Cees and Gomez, Francisco}, title = {Empirical Correlations for Geometry Build-Up of Fixed Wing Unmanned Air Vehicles}, series = {APISAT 2018: The Proceedings of the 2018 Asia-Pacific International Symposium on Aerospace Technology (APISAT 2018)}, booktitle = {APISAT 2018: The Proceedings of the 2018 Asia-Pacific International Symposium on Aerospace Technology (APISAT 2018)}, publisher = {Springer}, address = {Singapore}, isbn = {978-981-13-3305-7}, doi = {10.1007/978-981-13-3305-7_109}, pages = {1365 -- 1381}, year = {2019}, abstract = {The results of a statistical investigation of 42 fixed-wing, small to medium sized (20 kg-1000 kg) reconnaissance unmanned air vehicles (UAVs) are presented. Regression analyses are used to identify correlations of the most relevant geometry dimensions with the UAV's maximum take-off mass. The findings allow an empirical based geometry-build up for a complete unmanned aircraft by referring to its take-off mass only. This provides a bridge between very early design stages (initial sizing) and the later determination of shapes and dimensions. The correlations might be integrated into a UAV sizing environment and allow designers to implement more sophisticated drag and weight estimation methods in this process. Additional information on correlation factors for a rough drag estimation methodology indicate how this technique can significantly enhance the accuracy of early design iterations.}, language = {en} } @article{RibitschHeumannTrotschaetal.2011, author = {Ribitsch, Doris and Heumann, Sonja and Trotscha, Eva and Herrero Acero, Enrique and Greimel, Katrin and Leber, Regina and Birner-Gruenberger, Ruth and Deller, Sigrid and Eiteljoerg, Inge and Remler, Peter and Weber, Thomas and Siegert, Petra and Maurer, Karl-Heinz and Donelli, Ilaria and Freddi, Giuliano and Schwab, Helmut and Guebitz, Georg M.}, title = {Hydrolysis of polyethyleneterephthalate by p-nitrobenzylesterase from Bacillus subtilis}, series = {Biotechnology progress}, volume = {Vol. 27}, journal = {Biotechnology progress}, number = {Iss. 4}, publisher = {Wiley}, address = {Hoboken}, issn = {1520-6033 (E-Journal); 8756-7938 (Print)}, pages = {951 -- 960}, year = {2011}, language = {en} } @book{SchubaLinnhoffPopienReichletal.1996, author = {Schuba, Marko and Linnhoff-Popien, Claudia and Reichl, Peter and Schuba, Marko}, title = {Systemprogrammierung : Skript zur Vorlesung an der RWTH Aachen / Otto Spaniol ...}, publisher = {Verl. der Augustinus-Buchh.}, address = {Aachen}, isbn = {3-86073-470-9}, pages = {VIII, 166 S. : graph. Darst.}, year = {1996}, language = {de} } @inproceedings{UibelBlass2013, author = {Uibel, Thomas and Blaß, Hans Joachim}, title = {Joints with Dowel Type Fasteners in CLT structures}, series = {Focus solid timber solutions : European Conference on Cross Laminated Timber (CLT); May 21-22, 2013, Graz University of Technology, Austria}, booktitle = {Focus solid timber solutions : European Conference on Cross Laminated Timber (CLT); May 21-22, 2013, Graz University of Technology, Austria}, organization = {European Conference on Cross Laminated Timber <2013, Graz>}, isbn = {978-1-85790-181-8 ; 1-85790-181-9}, pages = {119 -- 134}, year = {2013}, language = {en} } @article{SchaeferHoefkenSchuba2011, author = {Schaefer, Thomas and H{\"o}fken, Hans-Wilhelm and Schuba, Marko}, title = {Windows Phone 7 from a Digital Forensics' Perspective}, publisher = {Springer}, address = {Berlin}, doi = {10.1007/978-3-642-35515-8_6}, year = {2011}, language = {en} } @inproceedings{BroennerHoefkenSchuba2016, author = {Broenner, Simon and H{\"o}fken, Hans-Wilhelm and Schuba, Marko}, title = {Streamlining extraction and analysis of android RAM images}, series = {Proceedings of the 2nd International Conference on Information Systems Security and Privacy - ICISSP}, booktitle = {Proceedings of the 2nd International Conference on Information Systems Security and Privacy - ICISSP}, publisher = {SciTePress}, address = {Set{\´u}bal}, organization = {ICISSP International Conference on Information Systems Security and Privacy <2, 2016, Rome, Italy>}, isbn = {978-989-758-167-0}, doi = {10.5220/0005652802550264}, pages = {255 -- 264}, year = {2016}, abstract = {The Android operating system powers the majority of the world's mobile devices and has been becoming increasingly important in day-to-day digital forensics. Therefore, technicians and analysts are in need of reliable methods for extracting and analyzing memory images from live Android systems. This paper takes different existing, extraction methods and derives a universal, reproducible, reliably documented method for both extraction and analysis. In addition the VOLIX II front-end for the Volatility Framework is extended with additional functionality to make the analysis of Android memory images easier for technically non-adept users.}, language = {en} } @article{SchubaReichlKesdoganetal.1998, author = {Schuba, Marko and Reichl, Peter and Kesdogan, Dogan and Jungh{\"a}rtchen, Klaus}, title = {Simulative Performance Evaluation of the Temporary Pseudonym Method for Protecting Location Information in GSM Networks / Reichl, P. ; Kesdogan, D. ; Jungh{\"a}rtchen, K. ; Schuba, M.}, series = {Computer Performance Evaluation - Modelling Techniques and Tools / Puigjaner, Ramon (eds.)}, journal = {Computer Performance Evaluation - Modelling Techniques and Tools / Puigjaner, Ramon (eds.)}, publisher = {Springer}, address = {Berlin}, isbn = {3-540-64949-2}, pages = {105 -- 116}, year = {1998}, language = {en} } @article{SchubaHermanns1994, author = {Schuba, Marko and Hermanns, Oliver}, title = {Modellierung von Multicastmechanismen zur Unterst{\"u}tzung von Gruppenkommunikation / Schuba, Marko ; Hermanns, Oliver}, series = {Neue Konzepte f{\"u}r die offene verteilte Verarbeitung : Tagungsband des 1. Arbeitstreffens an der RWTH Aachen, 5. September 1994 / Hrsg. des Bd.: Claudia Popien und Bernd Meyer}, journal = {Neue Konzepte f{\"u}r die offene verteilte Verarbeitung : Tagungsband des 1. Arbeitstreffens an der RWTH Aachen, 5. September 1994 / Hrsg. des Bd.: Claudia Popien und Bernd Meyer}, publisher = {Verl. der Augustinus-Buchh.}, address = {Aachen}, isbn = {3-86073-143-2}, pages = {137 -- 147}, year = {1994}, language = {de} } @article{SchubaHaverkortSchneider2000, author = {Schuba, Marko and Haverkort, Boudewijn R. and Schneider, Gaby}, title = {Performance evaluation of multicast communication in packet-switched networks / Schuba, Marko ; Haverkort, Boudewijn R. ; Schneider, Gaby}, series = {Performance Evaluation. 39 (2000), H. 1-4}, journal = {Performance Evaluation. 39 (2000), H. 1-4}, isbn = {0166-5316}, doi = {10.1016/S0166-5316(99)00058-9}, pages = {61 -- 80}, year = {2000}, language = {en} }