@article{TurekHeidenGuoetal.2010,
  author    = {Turek, Monik and Heiden, Wolfgang and Guo, Sharon and Riesen, Alfred and Schubert, J{\"u}rgen and Zander, Willi and Kr{\"u}ger, Peter and Keusgen, Michael and Sch{\"o}ning, Michael Josef},
  title     = {Simultaneous detection of cyanide and heavy metals for environmental analysis by means of µISEs},
  series = {Physica Status Solidi (A)},
  volume    = {207},
  journal   = {Physica Status Solidi (A)},
  number    = {4},
  publisher = {Wiley-VCH},
  address   = {Berlin},
  issn      = {1862-6300},
  doi       = {10.1002/pssa.200983303},
  pages     = {817 -- 823},
  year      = {2010},
  abstract  = {In environmental analysis, cyanide and heavy metals play an important role, because these substances are highly toxic for biological systems. They can lead to chronic and acute diseases. Due to the chemical properties of cyanide it is frequently used for industrial processes such as extraction of silver and gold. Heavy metals can be found as trace elements in nature and are often applied in industries e.g., galvanization processes. Up to now, cyanide and heavy metals can be detected by several sensors separately and their detection is often limited to laboratory investigations. In this publication, with regard to an in situ analysis, a new miniaturized silicon-based sensor system for the simultaneous detection of cyanide and heavy metals in aqueous solutions is presented that is based on chalcogenide glass-based micro ion-selective electrodes (µISEs). The µISEs are incorporated into a specially designed measuring system for the simultaneous detection of heavy metals and cyanide in solutions and validated by simultaneous measurements of Cu2+- and CN--ions, Cd2+- and CN-- ions and Pb2+- and CN--ions. The particular sensor system has shown good sensor properties in the µ-molar ion-concentration range. For simultaneous measurements in complex heavy metal and cyanide solutions an intelligent software using fuzzy logic is discussed.},
  language  = {en}
}
@article{KirchnerOberlaenderFriedrichetal.2010,
  author    = {Kirchner, Patrick and Oberl{\"a}nder, Jan and Friedrich, Peter and Rysstad, Gunnar and Berger, J{\"o}rg and Keusgen, Michael and Sch{\"o}ning, Michael Josef},
  title     = {Realization of a calorimetric gas sensor on polyimide foil for applications in aseptic food industry},
  series = {Procedia Engineering},
  volume    = {5},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2010.09.098},
  pages     = {264 -- 267},
  year      = {2010},
  abstract  = {A calorimetric gas sensor is presented for the monitoring of gas-phase H2O2 at elevated temperature during sterilization processes in aseptic food industry. The sensor consists of two temperature-sensitive thin-film resistances built up on a polyimide foil with a thickness of 25 μm, which are passivated with a layer of SU-8 photo resist and catalytically activated with manganese(IV) oxide. Instead of an active heating structure, the calorimetric sensor utilizes the elevated temperature of an evaporated H2O2 aerosol. In an experimental set-up, the sensor has shown a sensitivity of 4.78 °C/(\%v/v) in a H2O2 concentration range of 0 to 10\% v/v at an evaporation temperature of 240 ∘C. Furthermore, the sensor possesses the same, unchanged sensor signal even at varied evaporation temperatures of the gas stream. The sensor characterization demonstrates the suitability of the calorimetric gas sensor for monitoring the efficiency of sterilization processes.},
  language  = {en}
}
@inproceedings{KirchnerSpelthahnSchoeningetal.2010,
  author    = {Kirchner, Patrick and Spelthahn, Heiko and Sch{\"o}ning, Michael Josef and Henkel, Hartmut and Schneider, Andreas and Friedrich, Peter and Kolstad, Jens and Berger, J{\"o}rg},
  title     = {Realisierung eines Polyimid-basierten kalorimetrischen Gassensors zur Inline-{\"U}berwachung der H2O2-Konzentration in aseptischen Abf{\"u}llsystemen},
  series = {Tagungsband: Sensoren und Messsysteme 2010},
  booktitle = {Tagungsband: Sensoren und Messsysteme 2010},
  publisher = {VDE Verlag},
  address   = {Berlin},
  isbn      = {978-3-8007-3260-9},
  pages     = {607 -- 612},
  year      = {2010},
  abstract  = {In aseptischen Abf{\"u}llsystemen wird Wasserstoffperoxid in der Gasphase aufgrund der stark oxidativen Wirkung zur Packstoffentkeimung eingesetzt. Dabei wird die Effizienz der Entkeimung im Wesentlichen von der vorliegenden H2O2-Konzentration im Packstoff bestimmt. Zur Inline-{\"U}berwachung der H2O2-Konzentration wurde ein kalorimetrischer Gassensor auf Basis einer flexiblen Polyimidfolie aus temperatursensitiven D{\"u}nnschicht-Widerst{\"a}nden und Mangan(IV)-oxid als katalytische Transducerschicht realisiert. Der Sensor weist ein lineares Ansprechverhalten mit einer Sensitivit{\"a}t von 7,15 °C/Vol.-\% in einem H2O2-Konzentrationsbereich von 0 bis 8 Vol.-\% auf. Weiterhin wurde zur Auslesung des Sensorsignals eine RFID-Elektronik, bestehend aus einem Sensor-Tag und einer Sende-/Empfangseinheit ausgelegt, sowie eine Abfolge des Messzyklus aufgestellt. Im weiteren Verlauf soll der kalorimetrische Gassensor mit der RFID-Elektronik gekoppelt und in eine Testverpackung zur Inline-{\"U}berwachung der H2O2-Konzentration in aseptischen Abf{\"u}llsystemen implementiert werden.},
  language  = {de}
}
@article{BohrnStuetzFleischeretal.2010,
  author    = {Bohrn, Ulrich and St{\"u}tz, Evamaria and Fleischer, Maximilian and Sch{\"o}ning, Michael Josef},
  title     = {Real-time detection of CO by eukaryotic cells},
  series = {Procedia Engineering},
  volume    = {5},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  pages     = {17 -- 20},
  year      = {2010},
  abstract  = {In this contribution, we focus on the detection of toxic gases with living eukaryotic cells. A cell-based gas sensor system, able to measure the effects of direct exposure of gases to cells in real-time, was set up. Impedance data as well as oxygen consumption of Chinese hamster lung fibroblast cells (V79) were analysed upon exposure to carbon monoxide (CO). The CO (diluted in wet synthetic air) affects the cell respiration as indicated by an attenuated respiration signal after the CO exposure as well as an instant increase of the capacitive part of the impedance signal during the gas exposure.},
  language  = {en}
}
@article{BuniatyanAbouzarMartirosyanetal.2010,
  author    = {Buniatyan, Vahe V. and Abouzar, Maryam H. and Martirosyan, Norayr W. and Schubert, J{\"u}rgen and Gevorgian, Spartak and Sch{\"o}ning, Michael Josef and Poghossian, Arshak},
  title     = {pH-sensitive properties of barium strontium titanate (BST) thin films prepared by pulsed laser deposition technique},
  series = {Physica Status Solidi (A)},
  volume    = {207},
  journal   = {Physica Status Solidi (A)},
  number    = {4},
  publisher = {Wiley-VCH},
  address   = {Berlin},
  issn      = {1862-6300},
  doi       = {10.1002/pssa.200983310},
  pages     = {824 -- 830},
  year      = {2010},
  abstract  = {pH-sensitive properties of barium strontium titanate (BST) high-k thin films as alternative gate material for field-effect capacitive (bio-)chemical sensors based on an electrolyte-insulator-semiconductor system have been investigated. The BST films of different compositions (Ba0.31Sr0.69TiO3, Ba0.25Sr0.75TiO3 and Mg-doped Ba0.8Sr0.2Mg0.1Ti0.9O3) were deposited by pulsed laser deposition technique from targets fabricated by self-propagating high-temperature synthesis. The realised sensors have been electrochemically characterised by means of impedance-spectroscopy, capacitance-voltage and constant-capacitance method. The sensors possess a Nernstian-like pH sensitivity in the concentration range between pH 3 and 11 with a response time of 5-10 s. An equivalent circuit model for the BST-based capacitive field-effect sensor is discussed.},
  language  = {en}
}
@article{DegeringEggertPulsetal.2010,
  author    = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich},
  title     = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides},
  series = {Applied and environmental microbiology},
  volume    = {76},
  journal   = {Applied and environmental microbiology},
  number    = {19},
  publisher = {American Society for Microbiology},
  address   = {Washington, DC},
  issn      = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)},
  doi       = {10.1128/AEM.01146-10},
  pages     = {6370 -- 6378},
  year      = {2010},
  abstract  = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.},
  language  = {en}
}
@article{WagnerMiyamotoSchoeningetal.2010,
  author    = {Wagner, Torsten and Miyamoto, Ko-ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo},
  title     = {Novel combination of digital light processing (DLP) and light-addressable potentiometric sensors (LAPS) for flexible chemical imaging},
  series = {Procedia Engineering},
  volume    = {5},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2010.09.161},
  pages     = {520 -- 523},
  year      = {2010},
  abstract  = {Chemical imaging systems allow the visualisation of the distribution of chemical species on the sensor surface. This work represents a new flexible approach of read out in a light-addressable potentiometric sensor (LAPS) with the help of a digital light processing (DLP) set-up. The DLP, known well for video projectors, consists of a mirror-array MEMS device which allows fast and flexible generation of light patterns. With the help of these light patterns the sensor surface of the LAPS device can be read out sequentially in a raster like scheme (scanning LAPS). The DLP approach has several advantages compared to conventional scanning LAPS set-ups, e.g., the spot size, the shape and the intensity of the light pointer can be changed easily and no mechanical movement is necessary, which reduces the size of the set-up and increases the stability and speed of measurement.},
  language  = {en}
}
@inproceedings{WernerSpelthahnSchoeningetal.2010,
  author    = {Werner, Frederik and Spelthahn, Heiko and Sch{\"o}ning, Michael Josef and Krumbe, Christoph and Wagner, Torsten and Yoshinobu, Tatsuo and Keusgen, Michael},
  title     = {Neue Ansteuerungselektronik f{\"u}r LAPS-basierte Biosensoren zur gleichzeitig ortsaufgel{\"o}sten Messung der pH-Konzentration},
  series = {Tagungsband: Sensoren und Messsysteme 2010},
  booktitle = {Tagungsband: Sensoren und Messsysteme 2010},
  publisher = {VDE Verlag},
  address   = {Berlin},
  isbn      = {978-3-8007-3260-9},
  pages     = {109 -- 114},
  year      = {2010},
  abstract  = {Ein lichtadressierbarer potentiometrischer Sensor (LAPS) kann die Konzentration eines oder mehrerer Analyten ortsaufgel{\"o}st auf der Sensoroberfl{\"a}che nachweisen. Dazu wird mit einer modulierten Lichtquelle die Halbleiterstruktur des zu untersuchenden Bereiches angeregt und ein entsprechender Photostrom ausgelesen. Durch gleichzeitige Anregung mehrere Bereiche durch Lichtquellen mit unterschiedlichen Modulationsfrequenzen k{\"o}nnen diese auch zeitgleich ausgelesen werden. Mit der neuen, hier vorgestellten Ansteuerungselektronik integriert in einem "Field Programmable Gate Array" (FPGA) ist es m{\"o}glich, mehrere Leuchtquellen gleichzeitig mit unterschiedlichen, w{\"a}hrend der Laufzeit festlegbaren Frequenzen, Phasen und Lichtintensit{\"a}ten zu betreiben. Somit kann das Frequenzverhalten des Sensors untersucht und die Konzentration des Analyten {\"u}ber das Oberfl{\"a}chenpotential mit Hilfe von Strom/Spannungs-Kurven und Phase/Spannungs-Kurven bestimmt werden.},
  language  = {de}
}
@article{MiyamotoKanekoMatsuoetal.2010,
  author    = {Miyamoto, Ko-ichiro and Kaneko, Kazumi and Matsuo, Akira and Wagner, Torsten and Kanoh, Shin`ichiro and Sch{\"o}ning, Michael Josef and Yoshinobu, Tatsuo},
  title     = {Miniaturized chemical imaging sensor system using an OLED display panel},
  series = {Procedia Engineering},
  volume    = {5},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2010.09.160},
  pages     = {516 -- 519},
  year      = {2010},
  abstract  = {The chemical imaging sensor is a semiconductor-based chemical sensor that can visualize the two-dimensional distribution of specific ions or molecules in the solution. In this study, we developed a miniaturized chemical imaging sensor system with an OLED display panel as a light source that scans the sensor plate. In the proposed configuration, the display panel is placed directly below the sensor plate and illuminates the back surface. The measured area defined by illumination can be arbitrarily customized to fit the size and the shape of the sample to be measured. The waveform of the generated photocurrent, the currentvoltage characteristics and the pH sensitivity were investigated and pH imaging with this miniaturized system was demonstrated.},
  language  = {en}
}
@article{AbouzarPedrazaSchoeningetal.2010,
  author    = {Abouzar, Maryam H. and Pedraza, A. M. and Sch{\"o}ning, Michael Josef and Poghossian, Arshak},
  title     = {Label-free DNA hybridization and denaturation detection by means of field-effect nanoplate SOI capacitors functionalized with gold nanoparticles},
  series = {Procedia Engineering},
  volume    = {5},
  journal   = {Procedia Engineering},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {1877-7058},
  doi       = {10.1016/j.proeng.2010.09.259},
  pages     = {918 -- 921},
  year      = {2010},
  abstract  = {A new approach for a label-free electrical detection of DNA hybridization and denaturation using an array of individually addressable field-effect nanoplate SOI (silicon-on-insulator) capacitors functionalized with gold nanoparticles is presented. By using a constant-capacitance measuring setup in a differential mode, signal changes of ∼110 mV and ∼70 mV have been registered after the DNA hybridization and denaturation events, respectively.},
  language  = {en}
}