@article{DegeringEggertPulsetal.2010, author = {Degering, Christian and Eggert, Thorsten and Puls, Michael and Bongaerts, Johannes and Evers, Stefan and Maurer, Karl-Heinz and Jaeger, Karl-Erich}, title = {Optimization of protease secretion in Bacillus subtilis and Bacillus licheniformis by screening of homologous and herologous signal peptides}, series = {Applied and environmental microbiology}, volume = {76}, journal = {Applied and environmental microbiology}, number = {19}, publisher = {American Society for Microbiology}, address = {Washington, DC}, issn = {1098-5336 (E-Journal); 0003-6919 (Print); 0099-2240 (Print)}, doi = {10.1128/AEM.01146-10}, pages = {6370 -- 6378}, year = {2010}, abstract = {Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, which are recognized by the secretion machinery. Here, we have studied the production of an industrially important secreted protease, namely, subtilisin BPN′ from Bacillus amyloliquefaciens. One hundred seventy-three signal peptides originating from B. subtilis and 220 signal peptides from the B. licheniformis type strain were fused to this secretion target and expressed in B. subtilis, and the resulting library was analyzed by high-throughput screening for extracellular proteolytic activity. We have identified a number of signal peptides originating from both organisms which produced significantly increased yield of the secreted protease. Interestingly, we observed that levels of extracellular protease were improved not only in B. subtilis, which was used as the screening host, but also in two different B. licheniformis strains. To date, it is impossible to predict which signal peptide will result in better secretion and thus an improved yield of a given extracellular target protein. Our data show that screening a library consisting of homologous and heterologous signal peptides fused to a target protein can identify more-effective signal peptides, resulting in improved protein export not only in the original screening host but also in different production strains.}, language = {en} } @incollection{ReimerBraunWellmeretal.2010, author = {Reimer, Lars and Braun, Carsten and Wellmer, Georg and Behr, Marek and Ballmann, Josef}, title = {Development of a modular method for computational aero-structural analysis of aircraft}, series = {Summary of flow modulation and fluid-structure interaction findings. Results of the Collaborative Research Center SFB 401 at the RWTH Aachen University, Aachen, Germany, 1997-2008 / ed.: Wolfgang Schr{\"o}der. Notes on numerical fluid mechanics and multidisciplinary design. Vol. 109}, booktitle = {Summary of flow modulation and fluid-structure interaction findings. Results of the Collaborative Research Center SFB 401 at the RWTH Aachen University, Aachen, Germany, 1997-2008 / ed.: Wolfgang Schr{\"o}der. Notes on numerical fluid mechanics and multidisciplinary design. Vol. 109}, publisher = {Springer}, address = {Berlin}, isbn = {978-3-642-04087-0}, pages = {205 -- 238}, year = {2010}, language = {en} } @inproceedings{ElsenHawariJohnen2010, author = {Elsen, Ingo and Hawari, Asma and Johnen, Uwe}, title = {Produktkernel in der Systemintegration (Erfahrungsbericht aus der Praxis)}, series = {Vom Projekt zum Produkt - Fachtagung des GI-Fachausschusses Management der Anwendungsentwicklung und -wartung im Fachbereich Wirtschaftsinformatik (WI-MAW), 1. - 3. Dezember 2010 in Aachen}, booktitle = {Vom Projekt zum Produkt - Fachtagung des GI-Fachausschusses Management der Anwendungsentwicklung und -wartung im Fachbereich Wirtschaftsinformatik (WI-MAW), 1. - 3. Dezember 2010 in Aachen}, editor = {Pietsch, Wolfram and Krams, Benedikt}, publisher = {Gesellschaft f{\"u}r Informatik eV}, address = {Bonn}, isbn = {9783885792727}, issn = {1617-5468}, pages = {93 -- 102}, year = {2010}, abstract = {In der Vergangenheit basierten große Systemintegrationsprojekte in der Regel auf Individualentwicklungen f{\"u}r einzelne Kunden. Getrieben durch Kostendruck steigt aber der Bedarf nach standardisierten L{\"o}sungen, die gleichzeitig die individuellen Anforderungen des jeweiligen Umfelds ber{\"u}cksichtigen. T-Systems GEI GmbH wird beiden Anforderungen mit Produktkerneln gerecht. Neben den technischen Aspekten der Kernelentwicklung spielen besonders organisatorische Aspekte eine Rolle, um Kernel effizient und qualitativ hochwertig zu entwickeln, ohne deren Funktionalit{\"a}ten ins Uferlose wachsen zu lassen. Umgesetzt hat T-Systems dieses Konzept f{\"u}r Flughafeninformationssysteme. Damit kann dem wachsenden Bedarf der Flughafenbetreiber nach einer effizienten und kosteng{\"u}nstigen Softwarel{\"o}sung zur Unterst{\"u}tzung Ihrer Gesch{\"a}ftsprozesse entsprochen werden.}, language = {de} } @inproceedings{ParkButenwegReindl2010, author = {Park, Jin and Butenweg, Christoph and Reindl, Lukas}, title = {Untersuchung der Eignung von Ankerschienensystemen unter Erdbebeneinwirkungen}, series = {Conference proceedings / ANSYS Conference \& 28th CADFEM Users´ Meeting 2010 : 3. - 5. November 2010, Eurogess Aachen / CADFEM; ANSYS}, booktitle = {Conference proceedings / ANSYS Conference \& 28th CADFEM Users´ Meeting 2010 : 3. - 5. November 2010, Eurogess Aachen / CADFEM; ANSYS}, publisher = {CADFEM [u.a.]}, address = {Grafing}, isbn = {3-937523-07-3}, pages = {Dokument 2.11.1}, year = {2010}, language = {de} } @incollection{MeskourisGellertButenweg2010, author = {Meskouris, Konstantin and Gellert, Christoph and Butenweg, Christoph}, title = {Neuer Ansatz f{\"u}r die Bemessung von Mauerwerksbauten}, series = {Innovationen im konstruktiven Ingenieurbau : Methoden - Materialien - Bauwerke ; Festschrift Professor Hirschfeld / hrsg. von Karl Beucke}, booktitle = {Innovationen im konstruktiven Ingenieurbau : Methoden - Materialien - Bauwerke ; Festschrift Professor Hirschfeld / hrsg. von Karl Beucke}, publisher = {Bauhaus-Univ. Weimar}, address = {Weimar}, pages = {217 -- 224}, year = {2010}, language = {de} } @incollection{ButenwegGellertMeyer2010, author = {Butenweg, Christoph and Gellert, Christoph and Meyer, Udo}, title = {Erdbebenbemessung bei Mauerwerksbauten}, series = {Mauerwerk-Kalender 2010: Normen f{\"u}r Bemessung und Ausf{\"u}hrung}, booktitle = {Mauerwerk-Kalender 2010: Normen f{\"u}r Bemessung und Ausf{\"u}hrung}, publisher = {Ernst \& Sohn}, address = {Berlin}, isbn = {3-433-60040-6 ; 978-3-433-60040-5}, doi = {10.1002/9783433600405.ch7}, pages = {141 -- 167}, year = {2010}, language = {de} } @misc{O'ConnellSiegertEversetal.2010, author = {O'Connell, Timothy and Siegert, Petra and Evers, Stefan and Bongaerts, Johannes and Weber, Thomas and Maurer, Karl-Heinz and Bessler, Cornelius}, title = {Wasch- oder Reinigungsmittel mit gesteigerter Waschkraft [Offenlegungsschrift]}, publisher = {Deutsches Patentamt}, address = {M{\"u}nchen}, pages = {1 -- 34}, year = {2010}, language = {de} } @inproceedings{HeinzOertelBung2010, author = {Heinz, G. and Oertel, Mario and Bung, Daniel Bernhard}, title = {Investigations on a cooling water system for a gas tubine facility concerning air vortexes and sediment transport}, series = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, booktitle = {Hydraulic structures: useful water harvesting systems or relics? : Third International Junior Researcher and Engineer Workshop on Hydraulic Structures (IJREWHS'10) : Edinburgh, Scotland, U.K., 2-3 May 2010}, editor = {Janssen, Robert}, publisher = {School of Civil Engineering, The University of Queensland}, address = {Brisbane}, organization = {International Junior Researcher and Engineer Workshop on Hydraulic Structures <3, 2010, Edinburgh>}, isbn = {9781742720159}, pages = {109 -- 116}, year = {2010}, language = {en} } @incollection{Dachwald2010, author = {Dachwald, Bernd}, title = {Solar sail dynamics and control}, series = {Encyclopedia of Aerospace Engineering}, booktitle = {Encyclopedia of Aerospace Engineering}, publisher = {Wiley}, address = {Hoboken}, doi = {10.1002/9780470686652.eae292}, year = {2010}, abstract = {Solar sails are large and lightweight reflective structures that are propelled by solar radiation pressure. This chapter covers their orbital and attitude dynamics and control. First, the advantages and limitations of solar sails are discussed and their history and development status is outlined. Because the dynamics of solar sails is governed by the (thermo-)optical properties of the sail film, the basic solar radiation pressure force models have to be described and compared before parameters to measure solar sail performance can be defined. The next part covers the orbital dynamics of solar sails for heliocentric motion, planetocentric motion, and motion at Lagrangian equilibrium points. Afterwards, some advanced solar radiation pressure force models are described, which allow to quantify the thrust force on solar sails of arbitrary shape, the effects of temperature, of light incidence angle, of surface roughness, and the effects of optical degradation of the sail film in the space environment. The orbital motion of a solar sail is strongly coupled to its rotational motion, so that the attitude control of these soft and flexible structures is very challenging, especially for planetocentric orbits that require fast attitude maneuvers. Finally, some potential attitude control methods are sketched and selection criteria are given.}, language = {en} } @article{ZhubanovaAknazarovMansurovetal.2010, author = {Zhubanova, Azhar A. and Aknazarov, S. K. and Mansurov, Zulkhair and Digel, Ilya and Kozhalakova, A. A. and Akimbekov, Nuraly S. and O'Heras, Carlos and Tazhibayeva, S. and Artmann, Gerhard and Temiz Artmann, Ayseg{\"u}l}, title = {Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials}, year = {2010}, abstract = {Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.}, subject = {Kohlenstofffaser}, language = {en} }