@article{NiedermeierPennerUsherovichetal.2023,
  author    = {Niedermeier, Jana and Penner, Crystal and Usherovich, Samuel and B{\´e}langer-Champagne, Camille and Paulßen, Elisabeth and Hoehr, Cornelia},
  title     = {Optical Fibers as Dosimeter Detectors for Mixed Proton/Neutron Fields - A Biological Dosimeter},
  series = {electronics},
  volume    = {12},
  journal   = {electronics},
  number    = {2},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {2079-9292},
  doi       = {10.3390/electronics12020324},
  pages     = {11 Seiten},
  year      = {2023},
  abstract  = {In recent years, proton therapy has gained importance as a cancer treatment modality due to its conformality with the tumor and the sparing of healthy tissue. However, in the interaction of the protons with the beam line elements and patient tissues, potentially harmful secondary neutrons are always generated. To ensure that this neutron dose is as low as possible, treatment plans could be created to also account for and minimize the neutron dose. To monitor such a treatment plan, a compact, easy to use, and inexpensive dosimeter must be developed that not only measures the physical dose, but which can also distinguish between proton and neutron contributions. To that end, plastic optical fibers with scintillation materials (Gd₂O₂S:Tb, Gd₂O₂S:Eu, and YVO₄:Eu) were irradiated with protons and neutrons. It was confirmed that sensors with different scintillation materials have different sensitivities to protons and neutrons. A combination of these three scintillators can be used to build a detector array to create a biological dosimeter.},
  language  = {en}
}
@article{RuebbelkeVoegeleGrajewskietal.2023,
  author    = {R{\"u}bbelke, Dirk and V{\"o}gele, Stefan and Grajewski, Matthias and Zobel, Luzy},
  title     = {Cross border adjustment mechanism: Initial data for the assessment of hydrogen-based steel production},
  series = {Data in Brief},
  volume    = {47},
  journal   = {Data in Brief},
  number    = {Article 108907},
  publisher = {Elsevier},
  address   = {Amsterdam},
  issn      = {2352-3409},
  doi       = {10.1016/j.dib.2023.108907},
  pages     = {1 -- 5},
  year      = {2023},
  abstract  = {Ambitious climate targets affect the competitiveness of industries in the international market. To prevent such industries from moving to other countries in the wake of increased climate protection efforts, cost adjustments may become necessary. Their design requires knowledge of country-specific production costs. Here, we present country-specific cost figures for different production routes of steel, paying particular attention to transportation costs. The data can be used in floor price models aiming to assess the competitiveness of different steel production routes in different countries (R{\"u}bbelke, 2022).},
  language  = {en}
}
@article{HaegerGrankinWagner2023,
  author    = {Haeger, Gerrit and Grankin, Alina and Wagner, Michaela},
  title     = {Construction of an Aspergillus oryzae triple amylase deletion mutant as a chassis to evaluate industrially relevant amylases using multiplex CRISPR/Cas9 editing technology},
  series = {Applied Research},
  journal   = {Applied Research},
  number    = {Early View},
  publisher = {Wiley-VCH},
  issn      = {2702-4288},
  doi       = {10.1002/appl.202200106},
  pages     = {1 -- 15},
  year      = {2023},
  abstract  = {Aspergillus oryzae is an industrially relevant organism for the secretory production of heterologous enzymes, especially amylases. The activities of potential heterologous amylases, however, cannot be quantified directly from the supernatant due to the high background activity of native α-amylase. This activity is caused by the gene products of amyA, amyB, and amyC. In this study, an in vitro CRISPR/Cas9 system was established in A. oryzae to delete these genes simultaneously. First, pyrG of A. oryzae NSAR1 was mutated by exploiting NHEJ to generate a counter-selection marker. Next, all amylase genes were deleted simultaneously by co-transforming a repair template carrying pyrG of Aspergillus nidulans and flanking sequences of amylase gene loci. The rate of obtained triple knock-outs was 47\%. We showed that triple knockouts do not retain any amylase activity in the supernatant. The established in vitro CRISPR/Cas9 system was used to achieve sequence-specific knock-in of target genes. The system was intended to incorporate a single copy of the gene of interest into the desired host for the development of screening methods. Therefore, an integration cassette for the heterologous Fpi amylase was designed to specifically target the amyB locus. The site-specific integration rate of the plasmid was 78\%, with exceptional additional integrations. Integration frequency was assessed via qPCR and directly correlated with heterologous amylase activity. Hence, we could compare the efficiency between two different signal peptides. In summary, we present a strategy to exploit CRISPR/Cas9 for gene mutation, multiplex knock-out, and the targeted knock-in of an expression cassette in A. oryzae. Our system provides straightforward strain engineering and paves the way for development of fungal screening systems.},
  language  = {en}
}
@article{FayyaziSardarThomasetal.2023,
  author    = {Fayyazi, Mojgan and Sardar, Paramjotsingh and Thomas, Sumit Infent and Daghigh, Roonak and Jamali, Ali and Esch, Thomas and Kemper, Hans and Langari, Reza and Khayyam, Hamid},
  title     = {Artificial intelligence/machine learning in energy management systems, control, and optimization of hydrogen fuel cell vehicles},
  volume    = {15},
  number    = {6},
  publisher = {MDPI},
  address   = {Basel},
  doi       = {10.3390/su15065249},
  pages     = {38},
  year      = {2023},
  abstract  = {Environmental emissions, global warming, and energy-related concerns have accelerated the advancements in conventional vehicles that primarily use internal combustion engines. Among the existing technologies, hydrogen fuel cell electric vehicles and fuel cell hybrid electric vehicles may have minimal contributions to greenhouse gas emissions and thus are the prime choices for environmental concerns. However, energy management in fuel cell electric vehicles and fuel cell hybrid electric vehicles is a major challenge. Appropriate control strategies should be used for effective energy management in these vehicles. On the other hand, there has been significant progress in artificial intelligence, machine learning, and designing data-driven intelligent controllers. These techniques have found much attention within the community, and state-of-the-art energy management technologies have been developed based on them. This manuscript reviews the application of machine learning and intelligent controllers for prediction, control, energy management, and vehicle to everything (V2X) in hydrogen fuel cell vehicles. The effectiveness of data-driven control and optimization systems are investigated to evolve, classify, and compare, and future trends and directions for sustainability are discussed.},
  language  = {en}
}
@masterthesis{Kappel2023,
  type      = {Bachelor Thesis},
  author    = {Kappel, Jana Naomi},
  title     = {KLIKK : ein smartes Kinderprodukt f{\"u}r den altersgerechten Einstieg in die digitale Welt},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {163 Seiten},
  year      = {2023},
  abstract  = {Die neue Generation wird mit einer Medienwelt konfrontiert, welche nicht auf unsere kleinen Nutzer:innen ausgelegt ist. Die Verwendung von digitalen Medien hatte nie einen gr{\"o}ßeren Einfluss auf unsere Gesellschaft wie im Hier und Jetzt. Somit ist es von großer Relevanz, diese Nutzung kindergerechter zu gestalten und einen sicheren Rahmen f{\"u}r sie zu schaffen. Das Bachelorprojekt „KLIKK" legt einen L{\"o}sungsentwurf f{\"u}r den altersgerechten Einstieg f{\"u}r Kinder in diese digitale Welt vor, um den richtigen Umgang mit Smartwatches und darauf aufbauende Medien zu erm{\"o}glichen und gleichzeitig einen p{\"a}dagogischen Mehrwert zu bieten. Das didaktische Produktkonzept, welches explizit f{\"u}r Kinder designed wurde, beinhaltet das Erlernen des Uhrlesens und befriedigt das aufkommende Bed{\"u}rfnis nach Vernetzung in einem sicheren System. Zudem f{\"o}rdert es die Autonomie und ist an die kindliche Motorik angepasst. Mitinitiator und Namensgeber f{\"u}r diese Konzeption ist die deutsche Kinderuhrenmarke KWIO. KLIKK inkludiert Sicherheit, die Freude am Lernen und bietet eine M{\"o}glichkeit, die Kleinen ganz groß in unsere digitale Welt aufzunehmen.},
  language  = {de}
}
@article{MaurerRiekeSchemmetal.2023,
  author    = {Maurer, Florian and Rieke, Christian and Schemm, Ralf and Stollenwerk, Dominik},
  title     = {Analysis of an urban grid with high photovoltaic and e-mobility penetration},
  series = {Energies},
  volume    = {16},
  journal   = {Energies},
  number    = {8},
  publisher = {MDPI},
  address   = {Basel},
  issn      = {1996-1073},
  doi       = {10.3390/en16083380},
  pages     = {18 Seiten},
  year      = {2023},
  abstract  = {This study analyses the expected utilization of an urban distribution grid under high penetration of photovoltaic and e-mobility with charging infrastructure on a residential level. The grid utilization and the corresponding power flow are evaluated, while varying the control strategies and photovoltaic installed capacity in different scenarios. Four scenarios are used to analyze the impact of e-mobility. The individual mobility demand is modelled based on the largest German studies on mobility "Mobilit{\"a}t in Deutschland", which is carried out every 5 years. To estimate the ramp-up of photovoltaic generation, a potential analysis of the roof surfaces in the supply area is carried out via an evaluation of an open solar potential study. The photovoltaic feed-in time series is derived individually for each installed system in a resolution of 15 min. The residential consumption is estimated using historical smart meter data, which are collected in London between 2012 and 2014. For a realistic charging demand, each residential household decides daily on the state of charge if their vehicle requires to be charged. The resulting charging time series depends on the underlying behavior scenario. Market prices and mobility demand are therefore used as scenario input parameters for a utility function based on the current state of charge to model individual behavior. The aggregated electricity demand is the starting point of the power flow calculation. The evaluation is carried out for an urban region with approximately 3100 residents. The analysis shows that increased penetration of photovoltaics combined with a flexible and adaptive charging strategy can maximize PV usage and reduce the need for congestion-related intervention by the grid operator by reducing the amount of kWh charged from the grid by 30\% which reduces the average price of a charged kWh by 35\% to 14 ct/kWh from 21.8 ct/kWh without PV optimization. The resulting grid congestions are managed by implementing an intelligent price or control signal. The analysis took place using data from a real German grid with 10 subgrids. The entire software can be adapted for the analysis of different distribution grids and is publicly available as an open-source software library on GitHub.},
  language  = {en}
}
@masterthesis{Katemann2023,
  type      = {Bachelor Thesis},
  author    = {Katemann, Ronja Mairin},
  title     = {Gaming for everyone : gender-inklusives Gaming Equipment},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {139 Seiten},
  year      = {2023},
  abstract  = {Das Ausleben von Sexualit{\"a}t und Gender befindet sich im gesellschaftlichen Wandel. Vor allem junge Menschen lassen ihre Lebenswege nicht mehr von traditionellen Geschlechterrollen und -normen leiten. In einigen Bereichen ist das schon deutlich sichtbar, w{\"a}hrend andere Bereiche noch vor Herausforderungen stehen, wenn es um Inklusion, Akzeptanz und Repr{\"a}sentation geht. Besonders betroffen ist dabei der Gamingbereich, der repr{\"a}sentativ nach wie vor ein m{\"a}nnlich-dominiertes Gebiet zu sein scheint und durch Vorurteile gegen{\"u}ber Frauen und der LGBTQIA+ Community stigmatisiert ist. Die _FLUX Gaming Maus soll durch Gender-inklusives Design dazu beitragen, stereotype Rollenbilder in der Gaming Welt aufzubrechen und die Community nachhaltig wachsen zu lassen. Sie bietet dabei eine Alternative zu den „typisch m{\"a}nnlich" wirkenden Gaming Produkten auf dem Markt. Durch verschiedene Individualisierungsm{\"o}glichkeiten in Funktion und Gestalt kann _FLUX in einem Online-Konfigurator an den pers{\"o}nlichen Spielstil angepasst werden. So vermittelt _FLUX Zugeh{\"o}rigkeit und Repr{\"a}sentation unabh{\"a}ngig von Geschlecht und bietet Spaß am Gaming f{\"u}r jede:n.},
  language  = {de}
}
@masterthesis{Kilcher2023,
  type      = {Bachelor Thesis},
  author    = {Kilcher, Mathias},
  title     = {Barrierefreies Klettern : ein System zur Inklusion sehbehinderter Menschen in den Klettersport},
  publisher = {FH Aachen},
  address   = {Aachen},
  school      = {Fachhochschule Aachen},
  pages     = {135 Seiten},
  year      = {2023},
  abstract  = {Ziel des Projekts war die Entwicklung eines Systems, das es Menschen mit starker Sehbehinderung erm{\"o}glicht, Klettersport als ein Training f{\"u}r ihren K{\"o}rper und ihren Orientierungssinn nutzen zu k{\"o}nnen. Inklusion ist als gesellschaftliches Thema in den letzten Jahren stark in den Fokus ger{\"u}ckt. Dennoch ist es f{\"u}r Menschen mit Behinderung oft schwer, sozialen Anschluss zu finden. Dies wird zus{\"a}tzlich durch das mangelnde Angebot an inklusiven Freizeitaktivit{\"a}ten erschwert. Apollon erm{\"o}glicht Menschen mit starker Sehbehinderung die Orientierung w{\"a}hrend des Kletterns, indem es einerseits Informationen vermittelt, die normalerweise nur visuell wahrgenommen werden k{\"o}nnen und andererseits dabei hilft, sich besser an bereits gekletterte Routen zu erinnern. Das System setzt sich aus 3 Komponenten zusammen. Die Tritthilfen helfen der blinden Person dabei, auch kleine Tritte zu finden. Die taktilen Wegmarken stellen eine Art Checkpoint dar und helfen der blinden Person dabei, die Route in mehrere Abschnitte zu unterteilen. Gleichzeitig weisen sie darauf hin, welches Feature die kletternde Person erwartet. Die taktile Karte ist modular aufgebaut und setzt sich aus einer Legende und den Repr{\"a}sentanten der Wegmarken einer Route zusammen. Analog zu dem Betrachten der Route ist die blinde Person so vor dem Klettern in der Lage, Informationen {\"u}ber die Route zu erfahren.},
  language  = {de}
}
@incollection{EngelmannBaumann2023,
  author    = {Engelmann, Ulrich M. and Baumann, Martin},
  title     = {Moderationsexpertise f{\"u}r QMBs - die Methoden},
  series = {Qualit{\"a}tsmanagement im Gesundheitswesen},
  booktitle = {Qualit{\"a}tsmanagement im Gesundheitswesen},
  editor    = {Herbig, Nicola and Poppelreuter, Stefan},
  edition   = {60. Update},
  publisher = {T{\"U}V-Verlag},
  address   = {K{\"o}ln},
  isbn      = {978-3-8249-0714-4},
  pages     = {Kapitel 10815},
  year      = {2023},
  abstract  = {Damit Sie als Moderator effektiv und professionell moderieren k{\"o}nnen, sollten Sie die entsprechenden Methoden kennen. Mit den richtigen Methoden k{\"o}nnen Sie Diskussionen leiten, Konflikte l{\"o}sen, die Teilnehmer motivieren und daf{\"u}r sorgen, dass die Ziele der Veranstaltung erreicht werden. Außerdem helfen sie Ihnen, eine positive Atmosph{\"a}re zu schaffen und das Interesse der Teilnehmer zu halten. In diesem zweiten Beitrag der mehrteiligen Serie lernen Sie die grunds{\"a}tzlichen Methoden kennen, um erfolgreiche Teamsitzungen, Arbeitsgruppentreffen, Kick-offs und Meetings durchzuf{\"u}hren.},
  language  = {de}
}
@article{HaegerWirgesTanzmannetal.2023,
  author    = {Haeger, Gerrit and Wirges, Jessika and Tanzmann, Nicole and Oyen, Sven and Jolmes, Tristan and Jaeger, Karl-Erich and Sch{\"o}rken, Ulrich and Bongaerts, Johannes and Siegert, Petra},
  title     = {Chaperone assisted recombinant expression of a mycobacterial aminoacylase in Vibrio natriegens and Escherichia coli capable of N-lauroyl-L-amino acid synthesis},
  series = {Microbial Cell Factories},
  journal   = {Microbial Cell Factories},
  number    = {22},
  publisher = {Springer Nature},
  issn      = {1475-2859},
  doi       = {10.1186/s12934-023-02079-1},
  pages     = {Article number: 77 (2023)},
  year      = {2023},
  abstract  = {Background Aminoacylases are highly promising enzymes for the green synthesis of acyl-amino acids, potentially replacing the environmentally harmful Schotten-Baumann reaction. Long-chain acyl-amino acids can serve as strong surfactants and emulsifiers, with application in cosmetic industries. Heterologous expression of these enzymes, however, is often hampered, limiting their use in industrial processes. Results We identified a novel mycobacterial aminoacylase gene from Mycolicibacterium smegmatis MKD 8, cloned and expressed it in Escherichia coli and Vibrio natriegens using the T7 overexpression system. The recombinant enzyme was prone to aggregate as inclusion bodies, and while V. natriegens Vmax™ could produce soluble aminoacylase upon induction with isopropyl β-d-1-thiogalactopyranoside (IPTG), E. coli BL21 (DE3) needed autoinduction with lactose to produce soluble recombinant protein. We successfully conducted a chaperone co-expression study in both organisms to further enhance aminoacylase production and found that overexpression of chaperones GroEL/S enhanced aminoacylase activity in the cell-free extract 1.8-fold in V. natriegens and E. coli. Eventually, E. coli ArcticExpress™ (DE3), which co-expresses cold-adapted chaperonins Cpn60/10 from Oleispira antarctica, cultivated at 12 °C, rendered the most suitable expression system for this aminoacylase and exhibited twice the aminoacylase activity in the cell-free extract compared to E. coli BL21 (DE3) with GroEL/S co-expression at 20 °C. The purified aminoacylase was characterized based on hydrolytic activities, being most stable and active at pH 7.0, with a maximum activity at 70 °C, and stability at 40 °C and pH 7.0 for 5 days. The aminoacylase strongly prefers short-chain acyl-amino acids with smaller, hydrophobic amino acid residues. Several long-chain amino acids were fairly accepted in hydrolysis as well, especially N-lauroyl-L-methionine. To initially evaluate the relevance of this aminoacylase for the synthesis of N-acyl-amino acids, we demonstrated that lauroyl-methionine can be synthesized from lauric acid and methionine in an aqueous system. Conclusion Our results suggest that the recombinant enzyme is well suited for synthesis reactions and will thus be further investigated.},
  language  = {en}
}