Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-9958 Wissenschaftlicher Artikel Welden, Rene, welden@fh-aachen.de; Nagamine Komesu, Cindy A., ; Wagner, Patrick H., ; Schöning, Michael Josef, schoening@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de Photoelectrochemical enzymatic penicillin biosensor: A proof-of-concept experiment Photoelectrochemical (PEC) biosensors are a rather novel type of biosensors thatutilizelighttoprovideinformationaboutthecompositionofananalyte,enablinglight-controlled multi-analyte measurements. For enzymatic PEC biosensors,amperometric detection principles are already known in the literature. In con-trast, there is only a little information on H+-ion sensitive PEC biosensors. Inthis work, we demonstrate the detection of H+ions emerged by H+-generatingenzymes, exemplarily demonstrated with penicillinase as a model enzyme on atitanium dioxide photoanode. First, we describe the pH sensitivity of the sensorand study possible photoelectrocatalytic reactions with penicillin. Second, weshow the enzymatic PEC detection of penicillin. Weinheim Wiley-VCH 2021 4 Electrochemical Science Advances 2 Corresponding auhtor: Michael J. Schöning 4 1 5 10.1002/elsa.202100131 weltweit https://doi.org/10.1002/elsa.202100131 Fachbereich Medizintechnik und Technomathematik OPUS4-9509 Wissenschaftlicher Artikel Jablonski, Melanie, m.jablosnki@fh-aachen.de; Münstermann, Felix, ; Nork, Jasmina, ; Molinnus, Denise, Molinnus@fh-aachen.de; Muschallik, Lukas, muschallik@fh-aachen.de; Bongaerts, Johannes, bongaerts@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Keusgen, Michael, ; Siegert, Petra, siegert@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de Capacitive field-effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths An acetoin biosensor based on a capacitive electrolyte-insulator-semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance-voltage, and constant capacitance methods, respectively. Weinheim Wiley-VCH 2021 7 Seiten physica status solidi (a) applications and materials science 218 13 10.1002/pssa.202000765 weltweit https://doi.org/10.1002/pssa.202000765 Fachbereich Chemie und Biotechnologie OPUS4-9332 Wissenschaftlicher Artikel Özsoylu, Dua, oezsoylu@fh-aachen.de; Kizildag, Sefa, ; Schöning, Michael Josef, schoening@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de Differential chemical imaging of extracellular acidification within microfluidic channels using a plasma-functionalized light-addressable potentiometric sensor (LAPS) Extracellular acidification is a basic indicator for alterations in two vital metabolic pathways: glycolysis and cellular respiration. Measuring these alterations by monitoring extracellular acidification using cell-based biosensors such as LAPS plays an important role in studying these pathways whose disorders are associated with numerous diseases including cancer. However, the surface of the biosensors must be specially tailored to ensure high cell compatibility so that cells can represent more in vivo-like behavior, which is critical to gain more realistic in vitro results from the analyses, e.g., drug discovery experiments. In this work, O2 plasma patterning on the LAPS surface is studied to enhance surface features of the sensor chip, e.g., wettability and biofunctionality. The surface treated with O2 plasma for 30 s exhibits enhanced cytocompatibility for adherent CHO-K1 cells, which promotes cell spreading and proliferation. The plasma-modified LAPS chip is then integrated into a microfluidic system, which provides two identical channels to facilitate differential measurements of the extracellular acidification of CHO-K1 cells. To the best of our knowledge, it is the first time that extracellular acidification within microfluidic channels is quantitatively visualized as differential (bio-)chemical images. Amsterdam Elsevier 2020 8 Physics in Medicine 10 100030 10.1016/j.phmed.2020.100030 weltweit https://doi.org/10.1016/j.phmed.2020.100030 Fachbereich Medizintechnik und Technomathematik OPUS4-9258 Wissenschaftlicher Artikel Dantism, Shahriar, Dantism@fh-aachen.de; Röhlen, Desiree, roehlen@fh-aachen.de; Dahmen, Markus, ; Wagner, Torsten, torsten.wagner@fh-aachen.de; Wagner, Patrick, ; Schöning, Michael Josef, schoening@fh-aachen.de LAPS-based monitoring of metabolic responses of bacterial cultures in a paper fermentation broth As an alternative renewable energy source, methane production in biogas plants is gaining more and more attention. Biomass in a bioreactor contains different types of microorganisms, which should be considered in terms of process-stability control. Metabolically inactive microorganisms within the fermentation process can lead to undesirable, time-consuming and cost-intensive interventions. Hence, monitoring of the cellular metabolism of bacterial populations in a fermentation broth is crucial to improve the biogas production, operation efficiency, and sustainability. In this work, the extracellular acidification of bacteria in a paper-fermentation broth is monitored after glucose uptake, utilizing a differential light-addressable potentiometric sensor (LAPS) system. The LAPS system is loaded with three different model microorganisms (Escherichia coli, Corynebacterium glutamicum, and Lactobacillus brevis) and the effect of the fermentation broth at different process stages on the metabolism of these bacteria is studied. In this way, different signal patterns related to the metabolic response of microorganisms can be identified. By means of calibration curves after glucose uptake, the overall extracellular acidification of bacterial populations within the fermentation process can be evaluated. Amsterdam Elsevier 2020 Sensors and Actuators B: Chemical 320 Art. 128232 10.1016/j.snb.2020.128232 bezahl https://doi.org/10.1016/j.snb.2020.128232 Fachbereich Medizintechnik und Technomathematik OPUS4-9238 Wissenschaftlicher Artikel Welden, Rene, welden@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Wagner, Patrick H., ; Wagner, Torsten, torsten.wagner@fh-aachen.de Light-Addressable Electrodes for Dynamic and Flexible Addressing of Biological Systems and Electrochemical Reactions In this review article, we are going to present an overview on possible applications of light-addressable electrodes (LAE) as actuator/manipulation devices besides classical electrode structures. For LAEs, the electrode material consists of a semiconductor. Illumination with a light source with the appropiate wavelength leads to the generation of electron-hole pairs which can be utilized for further photoelectrochemical reaction. Due to recent progress in light-projection technologies, highly dynamic and flexible illumination patterns can be generated, opening new possibilities for light-addressable electrodes. A short introduction on semiconductor-electrolyte interfaces with light stimulation is given together with electrode-design approaches. Towards applications, the stimulation of cells with different electrode materials and fabrication designs is explained, followed by analyte-manipulation strategies and spatially resolved photoelectrochemical deposition of different material types. Basel MDPI 2020 21 Sensors 20 Artikel-Nummer 1680 1 22 10.3390/s20061680 weltweit https://doi.org/10.3390/s20061680 Fachbereich Medizintechnik und Technomathematik OPUS4-9236 Wissenschaftlicher Artikel Muschallik, Lukas, muschallik@fh-aachen.de; Molinnus, Denise, Molinnus@fh-aachen.de; Jablonski, Melanie, m.jablonski@fh-aachen.de; Kipp, Carina Ronja, ; Bongaerts, Johannes, bongaerts@fh-aachen.de; Pohl, Martina, ma.pohl@fz-juelich.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Selmer, Thorsten, selmer@fh-aachen.de; Siegert, Petra, siegert@fh-aachen.de Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. London RSC Publishing 2020 10 RSC Advances 10 12206 12216 10.1039/D0RA02066D weltweit https://doi.org/10.1039/D0RA02066D Fachbereich Chemie und Biotechnologie OPUS4-9126 Wissenschaftlicher Artikel Dantism, Shahriar, Dantism@fh-aachen.de; Röhlen, Desiree, roehlen@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Wagner, P., ; Schöning, Michael Josef, schoening@fh-aachen.de A LAPS-based differential sensor for parallelized metabolism monitoring of various bacteria Basel MDPI 2019 Sensors 19 21 Article number 4692 10.3390/s19214692 weltweit https://doi.org/10.3390/s19214692 Fachbereich Medizintechnik und Technomathematik OPUS4-9087 Wissenschaftlicher Artikel Arreola, Julio, arreola@fh-aachen.de; Keusgen, Michael, ; Wagner, Torsten, torsten.wagner@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de Combined calorimetric gas- and spore-based biosensor array for online monitoring and sterility assurance of gaseous hydrogen peroxide in aseptic filling machines Amsterdam Elsevier 2019 Biosensors and Bioelectronics 143 111628 10.1016/j.bios.2019.111628 bezahl https://doi.org/10.1016/j.bios.2019.111628 Fachbereich Medizintechnik und Technomathematik OPUS4-8976 Wissenschaftlicher Artikel Özsoylu, Dua, ; Kizildag, Sefa, dua.oezsoylu@alumni.fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de Effect of plasma treatment on the sensor properties of a light-addressable potentiometric sensor (LAPS) A light-addressable potentiometric sensor (LAPS) is a field-effect-based (bio-) chemical sensor, in which a desired sensing area on the sensor surface can be defined by illumination. Light addressability can be used to visualize the concentration and spatial distribution of the target molecules, e.g., H+ ions. This unique feature has great potential for the label-free imaging of the metabolic activity of living organisms. The cultivation of those organisms needs specially tailored surface properties of the sensor. O2 plasma treatment is an attractive and promising tool for rapid surface engineering. However, the potential impacts of the technique are carefully investigated for the sensors that suffer from plasma-induced damage. Herein, a LAPS with a Ta2O5 pH-sensitive surface is successfully patterned by plasma treatment, and its effects are investigated by contact angle and scanning LAPS measurements. The plasma duration of 30 s (30 W) is found to be the threshold value, where excessive wettability begins. Furthermore, this treatment approach causes moderate plasma-induced damage, which can be reduced by thermal annealing (10 min at 300 °C). These findings provide a useful guideline to support future studies, where the LAPS surface is desired to be more hydrophilic by O2 plasma treatment. Weinheim Wiley 2019 8 Seiten physica status solidi a : applications and materials sciences 216 Corresponding author: Torsten Wagner 20 10.1002/pssa.201900259 weltweit https://doi.org/10.1002/pssa.201900259 Fachbereich Medizintechnik und Technomathematik OPUS4-8972 Wissenschaftlicher Artikel Dantism, Shahriar, Dantism@fh-aachen.de; Röhlen, Desiree, roehlen@fh-aachen.de; Selmer, Thorsten, selmer@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Wagner, Patrick, ; Schöning, Michael Josef, schoening@fh-aachen.de Quantitative differential monitoring of the metabolic activity of Corynebacterium glutamicum cultures utilizing a light-addressable potentiometric sensor system Amsterdam Elsevier 2019 Biosensors and Bioelectronics 139 111332 10.1016/j.bios.2019.111332 bezahl https://doi.org/10.1016/j.bios.2019.111332 Fachbereich Medizintechnik und Technomathematik