Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-10715 Bericht Siegert, Petra, siegert@fh-aachen.de; Bongaerts, Johannes, bongaerts@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Selmer, Thorsten, selmer@fh-aachen.de Abschlussbericht zum Projekt zur Überwachung biotechnologischer Prozesse mittels Diacetyl-/Acetoin-Biosensor und Evaluierung von Acetoin-Reduktasen zur Verwendung in Biotransformationen Aachen 2022 16 Seiten Laufzeit: 01.01.2016 - 31.12.2019 (verlängert bis 31.12.2020) Förderkennzeichen: 322-8.03.04.02-FH-Struktur 2016/02 Gefördert durch: Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein-Westfalen Fachbereich Chemie und Biotechnologie OPUS4-9958 Wissenschaftlicher Artikel Welden, Rene, welden@fh-aachen.de; Nagamine Komesu, Cindy A., ; Wagner, Patrick H., ; Schöning, Michael Josef, schoening@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de Photoelectrochemical enzymatic penicillin biosensor: A proof-of-concept experiment Photoelectrochemical (PEC) biosensors are a rather novel type of biosensors thatutilizelighttoprovideinformationaboutthecompositionofananalyte,enablinglight-controlled multi-analyte measurements. For enzymatic PEC biosensors,amperometric detection principles are already known in the literature. In con-trast, there is only a little information on H+-ion sensitive PEC biosensors. Inthis work, we demonstrate the detection of H+ions emerged by H+-generatingenzymes, exemplarily demonstrated with penicillinase as a model enzyme on atitanium dioxide photoanode. First, we describe the pH sensitivity of the sensorand study possible photoelectrocatalytic reactions with penicillin. Second, weshow the enzymatic PEC detection of penicillin. Weinheim Wiley-VCH 2021 4 Electrochemical Science Advances 2 Corresponding auhtor: Michael J. Schöning 4 1 5 10.1002/elsa.202100131 weltweit https://doi.org/10.1002/elsa.202100131 Fachbereich Medizintechnik und Technomathematik OPUS4-9509 Wissenschaftlicher Artikel Jablonski, Melanie, m.jablosnki@fh-aachen.de; Münstermann, Felix, ; Nork, Jasmina, ; Molinnus, Denise, Molinnus@fh-aachen.de; Muschallik, Lukas, muschallik@fh-aachen.de; Bongaerts, Johannes, bongaerts@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Keusgen, Michael, ; Siegert, Petra, siegert@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de Capacitive field-effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths An acetoin biosensor based on a capacitive electrolyte-insulator-semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance-voltage, and constant capacitance methods, respectively. Weinheim Wiley-VCH 2021 7 Seiten physica status solidi (a) applications and materials science 218 13 10.1002/pssa.202000765 weltweit https://doi.org/10.1002/pssa.202000765 Fachbereich Chemie und Biotechnologie OPUS4-8976 Wissenschaftlicher Artikel Özsoylu, Dua, ; Kizildag, Sefa, dua.oezsoylu@alumni.fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de Effect of plasma treatment on the sensor properties of a light-addressable potentiometric sensor (LAPS) A light-addressable potentiometric sensor (LAPS) is a field-effect-based (bio-) chemical sensor, in which a desired sensing area on the sensor surface can be defined by illumination. Light addressability can be used to visualize the concentration and spatial distribution of the target molecules, e.g., H+ ions. This unique feature has great potential for the label-free imaging of the metabolic activity of living organisms. The cultivation of those organisms needs specially tailored surface properties of the sensor. O2 plasma treatment is an attractive and promising tool for rapid surface engineering. However, the potential impacts of the technique are carefully investigated for the sensors that suffer from plasma-induced damage. Herein, a LAPS with a Ta2O5 pH-sensitive surface is successfully patterned by plasma treatment, and its effects are investigated by contact angle and scanning LAPS measurements. The plasma duration of 30 s (30 W) is found to be the threshold value, where excessive wettability begins. Furthermore, this treatment approach causes moderate plasma-induced damage, which can be reduced by thermal annealing (10 min at 300 °C). These findings provide a useful guideline to support future studies, where the LAPS surface is desired to be more hydrophilic by O2 plasma treatment. Weinheim Wiley 2019 8 Seiten physica status solidi a : applications and materials sciences 216 Corresponding author: Torsten Wagner 20 10.1002/pssa.201900259 weltweit https://doi.org/10.1002/pssa.201900259 Fachbereich Medizintechnik und Technomathematik OPUS4-106 Konferenzveröffentlichung Wagner, Torsten, ; Schöning, Michael Josef, schoening@fh-aachen.de Preface of the Special Issue of I3S 2005 in Jülich (Germany) International Symposium on Sensor Science, I3S 2005 <3; 2005; Juelich, Germany> In: Sensors 2006, 6, 260-261 ISSN 1424-8220 2006 urn:nbn:de:hbz:a96-opus-1365 Fachbereich Medizintechnik und Technomathematik