Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-8381 Wissenschaftlicher Artikel Koch, Claudia, ; Poghossian, Arshak, poghossian@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Wege, Christian, Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts. Sydney Ivyspring 2018 12 Nanotheranostics 2 2 184 196 10.7150/ntno.22114 weltweit http://doi.org/10.7150/ntno.22114 Fachbereich Medizintechnik und Technomathematik OPUS4-9491 Wissenschaftlicher Artikel Jablonski, Melanie, m.jablonski@fh-aachen.de; Poghossian, Arshak, poghossian@fh-aachen.de; Severin, Robin, ; Keusgen, Michael, ; Wege, Christian, ; Schöning, Michael Josef, schoening@fh-aachen.de Capacitive Field-Effect Biosensor Studying Adsorption of Tobacco Mosaic Virus Particles Plant virus-like particles, and in particular, tobacco mosaic virus (TMV) particles, are increasingly being used in nano- and biotechnology as well as for biochemical sensing purposes as nanoscaffolds for the high-density immobilization of receptor molecules. The sensitive parameters of TMV-assisted biosensors depend, among others, on the density of adsorbed TMV particles on the sensor surface, which is affected by both the adsorption conditions and surface properties of the sensor. In this work, Ta₂O₅-gate field-effect capacitive sensors have been applied for the label-free electrical detection of TMV adsorption. The impact of the TMV concentration on both the sensor signal and the density of TMV particles adsorbed onto the Ta₂O₅-gate surface has been studied systematically by means of field-effect and scanning electron microscopy methods. In addition, the surface density of TMV particles loaded under different incubation times has been investigated. Finally, the field-effect sensor also demonstrates the label-free detection of penicillinase immobilization as model bioreceptor on TMV particles. MDPI 2021 15 Micromachines 12 1 1 16 10.3390/mi12010057 weltweit https://doi.org/10.3390/mi12010057 Fachbereich Medizintechnik und Technomathematik