Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-7821 Wissenschaftlicher Artikel Bäcker, Matthias, ; Koch, Claudia, ; Eiben, Sabine, ; Geiger, Fania, ; Eber, Fabian, ; Gliemann, Hartmut, ; Poghossian, Arshak, poghossian@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors Amsterdam Elsevier 2017 6 Sensors and Actuators B: Chemical 238 716 722 10.1016/j.snb.2016.07.096 Fachbereich Medizintechnik und Technomathematik OPUS4-8130 Konferenzveröffentlichung Jablonski, Melanie, ; Koch, Claudia, ; Bronder, Thomas, bronder@fh-aachen.de; Poghossian, Arshak, poghossian@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Field-Effect Biosensors Modified with Tobacco Mosaic Virus Nanotubes as Enzyme Nanocarrier 2017 4 MDPI Proceeding 1 Eurosensors 2017 Conference, Paris, France, 3-6 September 2017 4 10.3390/proceedings1040505 Fachbereich Medizintechnik und Technomathematik OPUS4-8429 Teil eines Buches Koch, Claudia, ; Poghossian, Arshak, poghossian@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Wege, Christina TMV-Based Adapter Templates for Enhanced Enzyme Loading in Biosensor Applications Nanotubular tobacco mosaic virus (TMV) particles and RNA-free lower-order coat protein (CP) aggregates have been employed as enzyme carriers in different diagnostic layouts and compared for their influence on biosensor performance. In the following, we describe a label-free electrochemical biosensor for improved glucose detection by use of TMV adapters and the enzyme glucose oxidase (GOD). A specific and efficient immobilization of streptavidin-conjugated GOD ([SA]-GOD) complexes on biotinylated TMV nanotubes or CP aggregates was achieved via bioaffinity binding. Glucose sensors with adsorptively immobilized [SA]-GOD, and with [SA]-GOD cross-linked with glutardialdehyde, respectively, were tested in parallel on the same sensor chip. Comparison of these sensors revealed that TMV adapters enhanced the amperometric glucose detection remarkably, conveying highest sensitivity, an extended linear detection range and fastest response times. These results underline a great potential of an integration of virus/biomolecule hybrids with electronic transducers for applications in biosensorics and biochips. Here, we describe the fabrication and use of amperometric sensor chips combining an array of circular Pt electrodes, their loading with GOD-modified TMV nanotubes (and other GOD immobilization methods), and the subsequent investigations of the sensor performance. New York, NY Humana Press 2018 15 Virus-Derived Nanoparticles for Advanced Technologies 978-1-4939-7808-3 Methods in Molecular Biology, vol 1776 553 568 10.1007/978-1-4939-7808-3 bezahl https://doi.org/10.1007/978-1-4939-7808-3 Fachbereich Medizintechnik und Technomathematik OPUS4-8343 Wissenschaftlicher Artikel Poghossian, Arshak, poghossian@fh-aachen.de; Jablonski, Melanie, m.jablonski@fh-aachen.de; Koch, Claudia, ; Bronder, Thomas, bronder@fh-aachen.de; Rolka, David, d.rolka@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Field-effect biosensor using virus particles as scaffolds for enzyme immobilization A field-effect biosensor employing tobacco mosaic virus (TMV) particles as scaffolds for enzyme immobilization is presented. Nanotubular TMV scaffolds allow a dense immobilization of precisely positioned enzymes with retained activity. To demonstrate feasibility of this new strategy, a penicillin sensor has been developed by coupling a penicillinase with virus particles as a model system. The developed field-effect penicillin biosensor consists of an Al-p-Si-SiO₂-Ta₂O₅-TMV structure and has been electrochemically characterized in buffer solutions containing different concentrations of penicillin G. In addition, the morphology of the biosensor surface with virus particles was characterized by scanning electron microscopy and atomic force microscopy methods. The sensors possessed a high penicillin sensitivity of ~ 92 mV/dec in a nearly-linear range from 0.1 mM to 10 mM, and a low detection limit of about 50 µM. The long-term stability of the penicillin biosensor was periodically tested over a time period of about one year without any significant loss of sensitivity. The biosensor has also been successfully applied for penicillin detection in bovine milk samples. Amsterdam Elsevier 2018 6 Biosensors and Bioelectronics 110 168 174 10.1016/j.bios.2018.03.036 bezahl http://doi.org/10.1016/j.bios.2018.03.036 Fachbereich Medizintechnik und Technomathematik OPUS4-9435 Wissenschaftlicher Artikel Poghossian, Arshak, poghossian@fh-aachen.de; Jablonski, Melanie, m.jablonski@fh-aachen.de; Molinnus, Denise, Molinnus@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Field-Effect Sensors for Virus Detection: From Ebola to SARS-CoV-2 and Plant Viral Enhancers Coronavirus disease 2019 (COVID-19) is a novel human infectious disease provoked by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific vaccines or drugs against COVID-19 are available. Therefore, early diagnosis and treatment are essential in order to slow the virus spread and to contain the disease outbreak. Hence, new diagnostic tests and devices for virus detection in clinical samples that are faster, more accurate and reliable, easier and cost-efficient than existing ones are needed. Due to the small sizes, fast response time, label-free operation without the need for expensive and time-consuming labeling steps, the possibility of real-time and multiplexed measurements, robustness and portability (point-of-care and on-site testing), biosensors based on semiconductor field-effect devices (FEDs) are one of the most attractive platforms for an electrical detection of charged biomolecules and bioparticles by their intrinsic charge. In this review, recent advances and key developments in the field of label-free detection of viruses (including plant viruses) with various types of FEDs are presented. In recent years, however, certain plant viruses have also attracted additional interest for biosensor layouts: Their repetitive protein subunits arranged at nanometric spacing can be employed for coupling functional molecules. If used as adapters on sensor chip surfaces, they allow an efficient immobilization of analyte-specific recognition and detector elements such as antibodies and enzymes at highest surface densities. The display on plant viral bionanoparticles may also lead to long-time stabilization of sensor molecules upon repeated uses and has the potential to increase sensor performance substantially, compared to conventional layouts. This has been demonstrated in different proof-of-concept biosensor devices. Therefore, richly available plant viral particles, non-pathogenic for animals or humans, might gain novel importance if applied in receptor layers of FEDs. These perspectives are explained and discussed with regard to future detection strategies for COVID-19 and related viral diseases. Lausanne Frontiers 2020 13 Frontiers in Plant Science 11 Article 598103 1 14 10.3389/fpls.2020.598103 weltweit https://doi.org/10.3389/fpls.2020.598103 Fachbereich Medizintechnik und Technomathematik OPUS4-10520 Wissenschaftlicher Artikel Welden, Melanie, ; Poghossian, Arshak, ; Vahidpour, Farnoosh, vahidpour@fh-aachen.de; Wendlandt, Tim, ; Keusgen, Michael, ; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO₂-Ta₂O₅ layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1-3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta₂O₅-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate. Basel MDPI 2022 Biosensors 12 This article belongs to the Special Issue "Biosensors: 10th Anniversary Feature Papers" 1 10.3390/bios12010043 weltweit https://doi.org/10.3390/bios12010043 Fachbereich Chemie und Biotechnologie OPUS4-10521 Wissenschaftlicher Artikel Welden, Rene, welden@fh-aachen.de; Jablonski, Melanie, ; Wege, Christina, ; Keusgen, Michael, ; Wagner, Patrick Hermann, ; Wagner, Torsten, torsten.wagner@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte's pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS. Basel MDPI 2021 Artikel 171 Biosensors 11 This article belongs to the Special Issue "Selected Papers from the 1st International Electronic Conference on Biosensors (IECB 2020)" 6 10.3390/bios11060171 weltweit https://doi.org/10.3390/bios11060171 Fachbereich Chemie und Biotechnologie OPUS4-10515 Wissenschaftlicher Artikel Welden, Melanie, ; Severins, Robin, ; Poghossian, Arshak, ; Wege, Christina, ; Bongaerts, Johannes, bongaerts@fh-aachen.de; Siegert, Petra, siegert@fh-aachen.de; Keusgen, Michael, ; Schöning, Michael Josef, schoening@fh-aachen.de Detection of acetoin and diacetyl by a tobacco mosaic virus-assisted field-effect biosensor Acetoin and diacetyl have a major impact on the flavor of alcoholic beverages such as wine or beer. Therefore, their measurement is important during the fermentation process. Until now, gas chromatographic techniques have typically been applied; however, these require expensive laboratory equipment and trained staff, and do not allow for online monitoring. In this work, a capacitive electrolyte-insulator-semiconductor sensor modified with tobacco mosaic virus (TMV) particles as enzyme nanocarriers for the detection of acetoin and diacetyl is presented. The enzyme acetoin reductase from Alkalihalobacillus clausii DSM 8716ᵀ is immobilized via biotin-streptavidin affinity, binding to the surface of the TMV particles. The TMV-assisted biosensor is electrochemically characterized by means of leakage-current, capacitance-voltage, and constant capacitance measurements. In this paper, the novel biosensor is studied regarding its sensitivity and long-term stability in buffer solution. Moreover, the TMV-assisted capacitive field-effect sensor is applied for the detection of diacetyl for the first time. The measurement of acetoin and diacetyl with the same sensor setup is demonstrated. Finally, the successive detection of acetoin and diacetyl in buffer and in diluted beer is studied by tuning the sensitivity of the biosensor using the pH value of the measurement solution. Basel MDPI 2022 Chemosensors 10 This article belongs to the Special Issue "Nanostructured Devices for Biochemical Sensing" 6 10.3390/chemosensors10060218 weltweit https://doi.org/10.3390/chemosensors10060218 Fachbereich Chemie und Biotechnologie OPUS4-10516 Konferenzveröffentlichung Welden, Melanie, ; Severins, Robin, severins@fh-aachen.de; Poghossian, Arshak, ; Wege, Christina, ; Siegert, Petra, siegert@fh-aachen.de; Keusgen, Michael, ; Schöning, Michael Josef, schoening@fh-aachen.de Studying the immobilization of acetoin reductase with Tobacco mosaic virus particles on capacitive field-effect sensors A capacitive electrolyte-insulator-semiconductor (EISCAP) biosensor modified with Tobacco mosaic virus (TMV) particles for the detection of acetoin is presented. The enzyme acetoin reductase (AR) was immobilized on the surface of the EISCAP using TMV particles as nanoscaffolds. The study focused on the optimization of the TMV-assisted AR immobilization on the Ta 2 O 5 -gate EISCAP surface. The TMV-assisted acetoin EISCAPs were electrochemically characterized by means of leakage-current, capacitance-voltage, and constant-capacitance measurements. The TMV-modified transducer surface was studied via scanning electron microscopy. IEEE 2022 4 Seiten 2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN) 978-1-6654-5860-3 (Online) IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), 29 May 2022 - 01 June 2022, Aveiro, Portugal. 10.1109/ISOEN54820.2022.9789657 campus https://doi.org/10.1109/ISOEN54820.2022.9789657 Fachbereich Medizintechnik und Technomathematik OPUS4-9965 Wissenschaftlicher Artikel Jablonski, Melanie, m.jablosnki@fh-aachen.de; Poghossian, Arshak, ; Keusgen, Michael, ; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Detection of plant virus particles with a capacitive field-effect sensor Plant viruses are major contributors to crop losses and induce high economic costs worldwide. For reliable, on-site and early detection of plant viral diseases, portable biosensors are of great interest. In this study, a field-effect SiO2-gate electrolyte-insulator-semiconductor (EIS) sensor was utilized for the label-free electrostatic detection of tobacco mosaic virus (TMV) particles as a model plant pathogen. The capacitive EIS sensor has been characterized regarding its TMV sensitivity by means of constant-capacitance method. The EIS sensor was able to detect biotinylated TMV particles from a solution with a TMV concentration as low as 0.025 nM. A good correlation between the registered EIS sensor signal and the density of adsorbed TMV particles assessed from scanning electron microscopy images of the SiO2-gate chip surface was observed. Additionally, the isoelectric point of the biotinylated TMV particles was determined via zeta potential measurements and the influence of ionic strength of the measurement solution on the TMV-modified EIS sensor signal has been studied. Cham Springer Nature 2021 9 Analytical and Bioanalytical Chemistry 413 Corresponding authors: Arshak Poghossian & Michael J. Schöning 5669 5678 10.1007/s00216-021-03448-8 weltweit https://doi.org/10.1007/s00216-021-03448-8 Fachbereich Medizintechnik und Technomathematik