Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-7821 Wissenschaftlicher Artikel Bäcker, Matthias, ; Koch, Claudia, ; Eiben, Sabine, ; Geiger, Fania, ; Eber, Fabian, ; Gliemann, Hartmut, ; Poghossian, Arshak, poghossian@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Tobacco mosaic virus as enzyme nanocarrier for electrochemical biosensors Amsterdam Elsevier 2017 6 Sensors and Actuators B: Chemical 238 716 722 10.1016/j.snb.2016.07.096 Fachbereich Medizintechnik und Technomathematik OPUS4-8381 Wissenschaftlicher Artikel Koch, Claudia, ; Poghossian, Arshak, poghossian@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Wege, Christian, Penicillin Detection by Tobacco Mosaic Virus-Assisted Colorimetric Biosensors The presentation of enzymes on viral scaffolds has beneficial effects such as an increased enzyme loading and a prolonged reusability in comparison to conventional immobilization platforms. Here, we used modified tobacco mosaic virus (TMV) nanorods as enzyme carriers in penicillin G detection for the first time. Penicillinase enzymes were conjugated with streptavidin and coupled to TMV rods by use of a bifunctional biotin-linker. Penicillinase-decorated TMV particles were characterized extensively in halochromic dye-based biosensing. Acidometric analyte detection was performed with bromcresol purple as pH indicator and spectrophotometry. The TMV-assisted sensors exhibited increased enzyme loading and strongly improved reusability, and higher analysis rates compared to layouts without viral adapters. They extended the half-life of the sensors from 4 - 6 days to 5 weeks and thus allowed an at least 8-fold longer use of the sensors. Using a commercial budget-priced penicillinase preparation, a detection limit of 100 µM penicillin was obtained. Initial experiments also indicate that the system may be transferred to label-free detection layouts. Sydney Ivyspring 2018 12 Nanotheranostics 2 2 184 196 10.7150/ntno.22114 weltweit http://doi.org/10.7150/ntno.22114 Fachbereich Medizintechnik und Technomathematik OPUS4-8343 Wissenschaftlicher Artikel Poghossian, Arshak, poghossian@fh-aachen.de; Jablonski, Melanie, m.jablonski@fh-aachen.de; Koch, Claudia, ; Bronder, Thomas, bronder@fh-aachen.de; Rolka, David, d.rolka@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Field-effect biosensor using virus particles as scaffolds for enzyme immobilization A field-effect biosensor employing tobacco mosaic virus (TMV) particles as scaffolds for enzyme immobilization is presented. Nanotubular TMV scaffolds allow a dense immobilization of precisely positioned enzymes with retained activity. To demonstrate feasibility of this new strategy, a penicillin sensor has been developed by coupling a penicillinase with virus particles as a model system. The developed field-effect penicillin biosensor consists of an Al-p-Si-SiO₂-Ta₂O₅-TMV structure and has been electrochemically characterized in buffer solutions containing different concentrations of penicillin G. In addition, the morphology of the biosensor surface with virus particles was characterized by scanning electron microscopy and atomic force microscopy methods. The sensors possessed a high penicillin sensitivity of ~ 92 mV/dec in a nearly-linear range from 0.1 mM to 10 mM, and a low detection limit of about 50 µM. The long-term stability of the penicillin biosensor was periodically tested over a time period of about one year without any significant loss of sensitivity. The biosensor has also been successfully applied for penicillin detection in bovine milk samples. Amsterdam Elsevier 2018 6 Biosensors and Bioelectronics 110 168 174 10.1016/j.bios.2018.03.036 bezahl http://doi.org/10.1016/j.bios.2018.03.036 Fachbereich Medizintechnik und Technomathematik OPUS4-10790 Wissenschaftlicher Artikel Wendlandt, Tim, ; Koch, Claudia, ; Britz, Beate, ; Liedek, Anke, ; Schmidt, Nora, ; Werner, Stefan, ; Gleba, Yuri, ; Vahidpour, Farnoosh, vahidpour@fh-aachen.de; Welden, Melanie, m.welden@fh-aachen.de; Poghossian, Arshak, ; Schöning, Michael Josef, schoening@fh-aachen.de Facile Purification and Use of Tobamoviral Nanocarriers for Antibody-Mediated Display of a Two-Enzyme System Immunosorbent turnip vein clearing virus (TVCV) particles displaying the IgG-binding domains D and E of Staphylococcus aureus protein A (PA) on every coat protein (CP) subunit (TVCVPA) were purified from plants via optimized and new protocols. The latter used polyethylene glycol (PEG) raw precipitates, from which virions were selectively re-solubilized in reverse PEG concentration gradients. This procedure improved the integrity of both TVCVPA and the wild-type subgroup 3 tobamovirus. TVCVPA could be loaded with more than 500 IgGs per virion, which mediated the immunocapture of fluorescent dyes, GFP, and active enzymes. Bi-enzyme ensembles of cooperating glucose oxidase and horseradish peroxidase were tethered together on the TVCVPA carriers via a single antibody type, with one enzyme conjugated chemically to its Fc region, and the other one bound as a target, yielding synthetic multi-enzyme complexes. In microtiter plates, the TVCVPA-displayed sugar-sensing system possessed a considerably increased reusability upon repeated testing, compared to the IgG-bound enzyme pair in the absence of the virus. A high coverage of the viral adapters was also achieved on Ta2O5 sensor chip surfaces coated with a polyelectrolyte interlayer, as a prerequisite for durable TVCVPA-assisted electrochemical biosensing via modularly IgG-assembled sensor enzymes. Basel MDPI 2023 Viruses 9 This article belongs to the Special Issue "Tobamoviruses 2023" 15 Artikel 1951 doi.org/10.3390/v15091951 weltweit https://doi.org/10.3390/v15091951 INB - Institut für Nano- und Biotechnologien