Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-9236 Wissenschaftlicher Artikel Muschallik, Lukas, muschallik@fh-aachen.de; Molinnus, Denise, Molinnus@fh-aachen.de; Jablonski, Melanie, m.jablonski@fh-aachen.de; Kipp, Carina Ronja, ; Bongaerts, Johannes, bongaerts@fh-aachen.de; Pohl, Martina, ma.pohl@fz-juelich.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Selmer, Thorsten, selmer@fh-aachen.de; Siegert, Petra, siegert@fh-aachen.de Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. London RSC Publishing 2020 10 RSC Advances 10 12206 12216 10.1039/D0RA02066D weltweit https://doi.org/10.1039/D0RA02066D Fachbereich Chemie und Biotechnologie OPUS4-9372 Wissenschaftlicher Artikel Muschallik, Lukas, muschallik@fh-aachen.de; Kipp, Carina Ronja, ; Recker, Inga, ; Bongaerts, Johannes, bongaerts@fh-aachen.de; Pohl, Martina, ; Gelissen, Melanie, gellissen@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Selmer, Thorsten, selmer@fh-aachen.de; Siegert, Petra, siegert@fh-aachen.de Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled. Amsterdam Elsevier 2020 9 Journal of Biotechnology 202 2590-1559 Vol. 324 61 70 10.1016/j.jbiotec.2020.09.016 bezahl https://doi.org/10.1016/j.jbiotec.2020.09.016 Fachbereich Chemie und Biotechnologie