Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-9965 Wissenschaftlicher Artikel Jablonski, Melanie, m.jablosnki@fh-aachen.de; Poghossian, Arshak, ; Keusgen, Michael, ; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Detection of plant virus particles with a capacitive field-effect sensor Plant viruses are major contributors to crop losses and induce high economic costs worldwide. For reliable, on-site and early detection of plant viral diseases, portable biosensors are of great interest. In this study, a field-effect SiO2-gate electrolyte-insulator-semiconductor (EIS) sensor was utilized for the label-free electrostatic detection of tobacco mosaic virus (TMV) particles as a model plant pathogen. The capacitive EIS sensor has been characterized regarding its TMV sensitivity by means of constant-capacitance method. The EIS sensor was able to detect biotinylated TMV particles from a solution with a TMV concentration as low as 0.025 nM. A good correlation between the registered EIS sensor signal and the density of adsorbed TMV particles assessed from scanning electron microscopy images of the SiO2-gate chip surface was observed. Additionally, the isoelectric point of the biotinylated TMV particles was determined via zeta potential measurements and the influence of ionic strength of the measurement solution on the TMV-modified EIS sensor signal has been studied. Cham Springer Nature 2021 9 Analytical and Bioanalytical Chemistry 413 Corresponding authors: Arshak Poghossian & Michael J. Schöning 5669 5678 10.1007/s00216-021-03448-8 weltweit https://doi.org/10.1007/s00216-021-03448-8 Fachbereich Medizintechnik und Technomathematik