Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-9509 Wissenschaftlicher Artikel Jablonski, Melanie, m.jablosnki@fh-aachen.de; Münstermann, Felix, ; Nork, Jasmina, ; Molinnus, Denise, Molinnus@fh-aachen.de; Muschallik, Lukas, muschallik@fh-aachen.de; Bongaerts, Johannes, bongaerts@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Keusgen, Michael, ; Siegert, Petra, siegert@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de Capacitive field-effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths An acetoin biosensor based on a capacitive electrolyte-insulator-semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance-voltage, and constant capacitance methods, respectively. Weinheim Wiley-VCH 2021 7 Seiten physica status solidi (a) applications and materials science 218 13 10.1002/pssa.202000765 weltweit https://doi.org/10.1002/pssa.202000765 Fachbereich Chemie und Biotechnologie OPUS4-10521 Wissenschaftlicher Artikel Welden, Rene, welden@fh-aachen.de; Jablonski, Melanie, ; Wege, Christina, ; Keusgen, Michael, ; Wagner, Patrick Hermann, ; Wagner, Torsten, torsten.wagner@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte's pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS. Basel MDPI 2021 Artikel 171 Biosensors 11 This article belongs to the Special Issue "Selected Papers from the 1st International Electronic Conference on Biosensors (IECB 2020)" 6 10.3390/bios11060171 weltweit https://doi.org/10.3390/bios11060171 Fachbereich Chemie und Biotechnologie