Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-8343 Wissenschaftlicher Artikel Poghossian, Arshak, poghossian@fh-aachen.de; Jablonski, Melanie, m.jablonski@fh-aachen.de; Koch, Claudia, ; Bronder, Thomas, bronder@fh-aachen.de; Rolka, David, d.rolka@fh-aachen.de; Wege, Christina, ; Schöning, Michael Josef, schoening@fh-aachen.de Field-effect biosensor using virus particles as scaffolds for enzyme immobilization A field-effect biosensor employing tobacco mosaic virus (TMV) particles as scaffolds for enzyme immobilization is presented. Nanotubular TMV scaffolds allow a dense immobilization of precisely positioned enzymes with retained activity. To demonstrate feasibility of this new strategy, a penicillin sensor has been developed by coupling a penicillinase with virus particles as a model system. The developed field-effect penicillin biosensor consists of an Al-p-Si-SiO₂-Ta₂O₅-TMV structure and has been electrochemically characterized in buffer solutions containing different concentrations of penicillin G. In addition, the morphology of the biosensor surface with virus particles was characterized by scanning electron microscopy and atomic force microscopy methods. The sensors possessed a high penicillin sensitivity of ~ 92 mV/dec in a nearly-linear range from 0.1 mM to 10 mM, and a low detection limit of about 50 µM. The long-term stability of the penicillin biosensor was periodically tested over a time period of about one year without any significant loss of sensitivity. The biosensor has also been successfully applied for penicillin detection in bovine milk samples. Amsterdam Elsevier 2018 6 Biosensors and Bioelectronics 110 168 174 10.1016/j.bios.2018.03.036 bezahl http://doi.org/10.1016/j.bios.2018.03.036 Fachbereich Medizintechnik und Technomathematik