Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-9236 Wissenschaftlicher Artikel Muschallik, Lukas, muschallik@fh-aachen.de; Molinnus, Denise, Molinnus@fh-aachen.de; Jablonski, Melanie, m.jablonski@fh-aachen.de; Kipp, Carina Ronja, ; Bongaerts, Johannes, bongaerts@fh-aachen.de; Pohl, Martina, ma.pohl@fz-juelich.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de; Selmer, Thorsten, selmer@fh-aachen.de; Siegert, Petra, siegert@fh-aachen.de Synthesis of α-hydroxy ketones and vicinal (R, R)-diols by Bacillus clausii DSM 8716ᵀ butanediol dehydrogenase α-hydroxy ketones (HK) and 1,2-diols are important building blocks for fine chemical synthesis. Here, we describe the R-selective 2,3-butanediol dehydrogenase from B. clausii DSM 8716ᵀ (BcBDH) that belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) and catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. Supplementation of Mn²⁺ (1 mM) increases BcBDH's activity in biotransformations. Furthermore, the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. London RSC Publishing 2020 10 RSC Advances 10 12206 12216 10.1039/D0RA02066D weltweit https://doi.org/10.1039/D0RA02066D Fachbereich Chemie und Biotechnologie OPUS4-9509 Wissenschaftlicher Artikel Jablonski, Melanie, m.jablosnki@fh-aachen.de; Münstermann, Felix, ; Nork, Jasmina, ; Molinnus, Denise, Molinnus@fh-aachen.de; Muschallik, Lukas, muschallik@fh-aachen.de; Bongaerts, Johannes, bongaerts@fh-aachen.de; Wagner, Torsten, torsten.wagner@fh-aachen.de; Keusgen, Michael, ; Siegert, Petra, siegert@fh-aachen.de; Schöning, Michael Josef, schoening@fh-aachen.de Capacitive field-effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths An acetoin biosensor based on a capacitive electrolyte-insulator-semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance-voltage, and constant capacitance methods, respectively. Weinheim Wiley-VCH 2021 7 Seiten physica status solidi (a) applications and materials science 218 13 10.1002/pssa.202000765 weltweit https://doi.org/10.1002/pssa.202000765 Fachbereich Chemie und Biotechnologie