Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-9105 Wissenschaftlicher Artikel Digel, Ilya, digel@fh-aachen.de; Wehlitz, V., ; Kayser, Peter, kayser@fh-aachen.de; Figiel-Lange, A., ; Bassam, R., ; Rundstedt, F. von, Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel 'suspension depletion' approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10-12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach. Oxford Wiley 2018 7 Plant Pathology 67 2 358 365 10.1111/ppa.12750 weltweit https://doi.org/10.1111/ppa.12750 Fachbereich Medizintechnik und Technomathematik