Dokument-ID Dokumenttyp Verfasser/Autoren Herausgeber Haupttitel Abstract Auflage Verlagsort Verlag Erscheinungsjahr Seitenzahl Schriftenreihe Titel Schriftenreihe Bandzahl ISBN Quelle der Hochschulschrift Konferenzname Bemerkung Quelle:Titel Quelle:Jahrgang Quelle:Heftnummer Quelle:Erste Seite Quelle:Letzte Seite URN DOI Zugriffsart Link Abteilungen OPUS4-10891 Wissenschaftlicher Artikel Uysal, Karya, uysal@fh-aachen.de; Firat, Ipek Serat, firat@fh-aachen.de; Creutz, Till, till.creutz@fh-aachen.de; Aydin, Inci Cansu, ; Artmann, Gerhard, artmann@fh-aachen.de; Teusch, Nicole, ; Temiz Artmann, Aysegül, a.artmann@fh-aachen.de A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here. Basel MDPI 2022 Artikel 22 membranes 2023 This article belongs to the Special Issue "Latest Scientific Discoveries in Polymer Membranes" 13(1) 10.3390/membranes13010022 weltweit https://doi.org/10.3390/membranes13010022 Fachbereich Medizintechnik und Technomathematik OPUS4-9014 Wissenschaftlicher Artikel Leschinger, Tim, ; Besch, Katharina, ; Aydin, Cansu, ; Staat, Manfred, m.staat@fh-aachen.de; Scaal, Martin, ; Müller, Lars Peter, ; Wegmann, Kilian, Irreparable rotator cuff tears: a biomechanical comparison of superior capsuloligamentous complex reconstruction techniques and an interposition graft technique 2019 4 The Orthopaedic Journal of Sports Medicine 7 8 1 5 10.1177/2325967119864590 weltweit https://doi.org/10.1177/2325967119864590 Fachbereich Medizintechnik und Technomathematik