TY - CHAP A1 - Bayer, Robin A1 - Hescheler, Jürgen A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül ED - Staat, Manfred ED - Erni, Daniel T1 - Treating arterial hypertension in a cell culture well T2 - 3rd YRA MedTech Symposium 2019 : May 24 / 2019 / FH AachenW N2 - Hypertension describes the pathological increase of blood pressure, which is most commonly associated with the increase of vascular wall stiffness [1]. Referring to the “Deutsche Bluthochdruck Liga” this pathology shows a growing trend in our aging society. In order to find novel pharmacological and probably personalized treatments, we want to present a functional approach to study biomechanical properties of a human aortic vascular model. In this method review we will give an overview of recent studies which were carried out with the CellDrum technology [2] and underline the added value to already existing standard procedures known from the field of physiology. Herein described CellDrum technology is a system to measure functional mechanical properties of cell monolayers and thin tissue constructs in-vitro. Additionally, the CellDrum enables to elucidate the mechanical response of cells to pharmacological drugs, toxins and vasoactive agents. Due to its highly flexible polymer support, cells can also be mechanically stimulated by steady and cyclic biaxial stretching. Y1 - 2019 SN - 978-3-940402-22-6 U6 - http://dx.doi.org/10.17185/duepublico/48750 SP - 5 EP - 6 PB - Universität Duisburg-Essen CY - Duisburg ER - TY - JOUR A1 - Temiz Artmann, Aysegül A1 - Kurulgan demirci, Eylem A1 - Fırat, Ipek Seda A1 - Oflaz, Hakan A1 - Artmann, Gerhard T1 - Recombinant activated protein C (rhAPC) affects lipopolysaccharide-induced mechanical compliance changes and beat frequency of mESC-derived cardiomyocyte monolayers JF - SHOCK KW - Septic cardiomyopathy KW - LPS KW - cardiomyocyte biomechanics KW - CellDrum KW - actin cytoskeleton Y1 - 2021 U6 - http://dx.doi.org/10.1097/SHK.0000000000001845 SN - 1540-0514 PB - Wolters Kluwer CY - Köln ER - TY - JOUR A1 - Uysal, Karya A1 - Creutz, Till A1 - Firat, Ipek Seda A1 - Artmann, Gerhard A1 - Teusch, Nicole A1 - Temiz Artmann, Aysegül T1 - Bio-functionalized ultra-thin, large-area and waterproof silicone membranes for biomechanical cellular loading and compliance experiments JF - Polymers N2 - Biocompatibility, flexibility and durability make polydimethylsiloxane (PDMS) membranes top candidates in biomedical applications. CellDrum technology uses large area, <10 µm thin membranes as mechanical stress sensors of thin cell layers. For this to be successful, the properties (thickness, temperature, dust, wrinkles, etc.) must be precisely controlled. The following parameters of membrane fabrication by means of the Floating-on-Water (FoW) method were investigated: (1) PDMS volume, (2) ambient temperature, (3) membrane deflection and (4) membrane mechanical compliance. Significant differences were found between all PDMS volumes and thicknesses tested (p < 0.01). They also differed from the calculated values. At room temperatures between 22 and 26 °C, significant differences in average thickness values were found, as well as a continuous decrease in thicknesses within a 4 °C temperature elevation. No correlation was found between the membrane thickness groups (between 3–4 µm) in terms of deflection and compliance. We successfully present a fabrication method for thin bio-functionalized membranes in conjunction with a four-step quality management system. The results highlight the importance of tight regulation of production parameters through quality control. The use of membranes described here could also become the basis for material testing on thin, viscous layers such as polymers, dyes and adhesives, which goes far beyond biological applications. Y1 - 2022 SN - 2073-4360 VL - 14 IS - 11 SP - 2213 PB - MDPI CY - Basel ER - TY - JOUR A1 - Uysal, Karya A1 - Firat, Ipek Serat A1 - Creutz, Till A1 - Aydin, Inci Cansu A1 - Artmann, Gerhard A1 - Teusch, Nicole A1 - Temiz Artmann, Aysegül T1 - A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes JF - membranes N2 - Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here. Y1 - 2022 U6 - http://dx.doi.org/10.3390/membranes13010022 N1 - This article belongs to the Special Issue "Latest Scientific Discoveries in Polymer Membranes" VL - 2023 IS - 13(1) PB - MDPI CY - Basel ER - TY - JOUR A1 - Staat, Manfred A1 - Kühn, R. A1 - Hauger, W. A1 - Sponagel, Stefan T1 - An Interpretation of Wolff’s Law JF - Biomedizinische Technik. 49 (2004) Y1 - 2004 SN - 0932-4666 N1 - Ergänzungsband 2, Teil 2 SP - 1020 EP - 1021 ER - TY - JOUR A1 - Staat, Manfred A1 - Baroud, G. A1 - Topcu, M. A1 - Sponagel, Stefan T1 - Soft Materials in Technology and Biology – Characteristics, Properties, and Parameter Identification JF - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.) Y1 - 2008 SN - 978-3-540-75408-4 SP - 253 EP - 315 PB - Springer CY - Berlin ER - TY - JOUR A1 - Staat, Manfred A1 - Sponagel, Stefan A1 - Nguyen, Nhu Huynh T1 - Experiment and material model for soft tissue materials JF - Constitutive models for rubber VI : proceedings of the sixth European Conference on Constitutive Models for Rubber, Dresden, Germany, 7 - 10 September 2009 / eds. Gert Heinrich ... Y1 - 2010 SN - 978-0-415-56327-7 SP - 465 EP - 470 PB - CRC Press CY - Boca Raton [u.a.] ER - TY - JOUR A1 - Kühn, Raoul-Roman A1 - Haugner, Werner A1 - Staat, Manfred A1 - Sponagel, Stefan T1 - A Two Phase Mixture Model based on Bone Observation N2 - An optimization method is developed to describe the mechanical behaviour of the human cancellous bone. The method is based on a mixture theory. A careful observation of the behaviour of the bone material leads to the hypothesis that the bone density is controlled by the principal stress trajectories (Wolff’s law). The basic idea of the developed method is the coupling of a scalar value via an eigenvalue problem to the principal stress trajectories. On the one hand this theory will permit a prediction of the reaction of the biological bone structure after the implantation of a prosthesis, on the other hand it may be useful in engineering optimization problems. An analytical example shows its efficiency. KW - Knochen KW - Knochenbildung KW - Knochenchirugie KW - Strukturanalyse KW - Schwammknochen KW - Knochendichte KW - Wolffsches Gesetz KW - bone structure KW - bone density KW - Wolff's Law KW - cancellous bone Y1 - 2004 ER - TY - JOUR A1 - Doorschodt, B. M. A1 - Schreinemachers, M. C. J. M. A1 - Behbahani, Mehdi A1 - Florquin, S. A1 - Weis, J. A1 - Staat, Manfred A1 - Tolba, R. H. T1 - Hypothermic machine perfusion of kidney grafts: which pressure is preferred JF - Annals of Biomedical Engineering. 39 (2011), H. 3 Y1 - 2011 SN - 1573-9686 SP - 1051 EP - 1059 PB - Springer CY - Berlin ER - TY - JOUR A1 - Temiz Artmann, Aysegül A1 - Kayatekin, Muammer B. A1 - Uysal, N. A1 - Resmi, H. T1 - Does antioxidant supplementation alter the effects of acute exercise on erythrocyte aggregation, deformability and endothelium adhesion in untrained rats? Kayatekin, Muammer B.; Uysal N.; Resmi, H.; Bediz, Seref C.; Temiz Artmann, A.; Genç, S.; Tugyan, K. JF - International Journal for Vitamin and Nutrition Research. 75 (2005), H. 4 Y1 - 2005 SN - 0300-9831 SP - 243 EP - 250 ER -