TY  - JOUR
A1  - Uysal, Karya
A1  - Firat, Ipek Serat
A1  - Creutz, Till
A1  - Aydin, Inci Cansu
A1  - Artmann, Gerhard
A1  - Teusch, Nicole
A1  - Temiz Artmann, Aysegül
T1  - A novel in vitro wound healing assay using free-standing, ultra-thin PDMS membranes
JF  - membranes
N2  - Advances in polymer science have significantly increased polymer applications in life sciences. We report the use of free-standing, ultra-thin polydimethylsiloxane (PDMS) membranes, called CellDrum, as cell culture substrates for an in vitro wound model. Dermal fibroblast monolayers from 28- and 88-year-old donors were cultured on CellDrums. By using stainless steel balls, circular cell-free areas were created in the cell layer (wounding). Sinusoidal strain of 1 Hz, 5% strain, was applied to membranes for 30 min in 4 sessions. The gap circumference and closure rate of un-stretched samples (controls) and stretched samples were monitored over 4 days to investigate the effects of donor age and mechanical strain on wound closure. A significant decrease in gap circumference and an increase in gap closure rate were observed in trained samples from younger donors and control samples from older donors. In contrast, a significant decrease in gap closure rate and an increase in wound circumference were observed in the trained samples from older donors. Through these results, we propose the model of a cell monolayer on stretchable CellDrums as a practical tool for wound healing research. The combination of biomechanical cell loading in conjunction with analyses such as gene/protein expression seems promising beyond the scope published here.
Y1  - 2022
U6  - http://dx.doi.org/10.3390/membranes13010022
N1  - This article belongs to the Special Issue "Latest Scientific Discoveries in Polymer Membranes"
VL  - 2023
IS  - 13(1)
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Zhubanova, Azhar A.
A1  - Digel, Ilya
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology
N2  - The most of conventional methods of air purification use the power of a fan to draw in air and pass it through a filter. The problem of bacterial contamination of inner parts of such a type of air conditioners in some cases draws attention towards alternative air-cleaning systems. Some manufacturers offer to use the ozone's bactericidal and deodorizing effects, but the wide spreading of such systems is restricted by the fact that toxic effects of ozone in respect of human beings are well known. In 2000 Sharp Inc. introduced "Plasma Cluster Ions (PCI)" air purification technology, which uses plasma discharge to generate cluster ions (I 0-14 ). This technology has been developed for those customers that are conscious about health and hygiene. In our experiments, we focused on some principal aspects of plasma-generated ions application - time-dependency and irreversibility of bactericidal action, spatial and kinetic characteristics of emitted cluster particles, their chemical targets in the microbial cells.
KW  - Clusterion
KW  - Raumluft
KW  - Luftreiniger
KW  - Plasmacluster ion technology
KW  - Air purification
Y1  - 2007
ER  - 
TY  - CHAP
A1  - Bassam, Rasha
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Effect of nitric oxide on protein thermal stability : [abstract]
N2  - As a deduction from these results, we can conclude that proteins mainly in vitro, denaturate totally at a temperature between 57°C -62°C, and they also affected by NO and different ions types. In which mainly, NO cause earlier protein denaturation, which means that, NO has a destabilizing effect on proteins, and also different ions will alter the protein denaturation in which, some ions will cause earlier protein denaturation while others not.
KW  - Stickstoffmonoxid
KW  - Proteine
KW  - Hämoglobin
KW  - nitric oxide gas
KW  - protein
KW  - hemoglobin
Y1  - 2009
ER  - 
TY  - CHAP
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Zaccai, Joe
A1  - Büldt, Georg
T1  - Temperature transitions of hemoglobin and cytosolic water diffusion in human red blood cells : [poster]
N2  - Background Hemoglobin interactions in red blood cells Hemoglobin dynamics in human red blood cells Diffusion of H2O in red blood cells
KW  - Erythrozyt
KW  - Hämoglobin
KW  - cytosolic water diffusion
KW  - hemoglobin dynamics
Y1  - 2007
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Zerlin, Kay
A1  - Digel, Ilya
T1  - Hemoglobin Senses Body Temperature
JF  - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)
Y1  - 2008
SN  - 978-3-540-75408-4
SP  - 415
EP  - 447
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Stadler, A.  M.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Embs, Jan P.
A1  - Zaccai, Joe
A1  - Büldt, Georg
T1  - Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
JF  - Biophysical Journal. 95 (2008), H. 11
Y1  - 2008
SN  - 1542-0086
SP  - 5449
EP  - 5461
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Garvey, G. J.
A1  - Bocahut, A.
A1  - Sacquin-Mora, S.
A1  - Digel, Ilya
A1  - Schneider, G. J.
A1  - Natali, F.
A1  - Artmann, Gerhard
A1  - Zaccai, G.
T1  - Thermal fluctuations of haemoglobin from different species : adaptation to temperature via conformational dynamics
JF  - Journal of the Royal Society Interface
N2  - Thermodynamic stability, configurational motions and internal forces of haemoglobin (Hb) of three endotherms (platypus, Ornithorhynchus anatinus; domestic chicken, Gallus gallus domesticus and human, Homo sapiens) and an ectotherm (salt water crocodile, Crocodylus porosus) were investigated using circular dichroism, incoherent elastic neutron scattering and coarse-grained Brownian dynamics simulations. The experimental results from Hb solutions revealed a direct correlation between protein resilience, melting temperature and average body temperature of the different species on the 0.1 ns time scale. Molecular forces appeared to be adapted to permit conformational fluctuations with a root mean square displacement close to 1.2 Å at the corresponding average body temperature of the endotherms. Strong forces within crocodile Hb maintain the amplitudes of motion within a narrow limit over the entire temperature range in which the animal lives. In fully hydrated powder samples of human and chicken, Hb mean square displacements and effective force constants on the 1 ns time scale showed no differences over the whole temperature range from 10 to 300 K, in contrast to the solution case. A complementary result of the study, therefore, is that one hydration layer is not sufficient to activate all conformational fluctuations of Hb in the pico- to nanosecond time scale which might be relevant for biological function. Coarse-grained Brownian dynamics simulations permitted to explore residue-specific effects. They indicated that temperature sensing of human and chicken Hb occurs mainly at residues lining internal cavities in the β-subunits.
Y1  - 2012
U6  - http://dx.doi.org/10.1098/rsif.2012.0364
SN  - 1742-5689
VL  - 9
IS  - 76
SP  - 2845
EP  - 2855
PB  - The Royal Society
CY  - London
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Digel, Ilya
A1  - Embs, Jan P.
A1  - Unruh, Tobias
A1  - Tehei, M.
A1  - Zaccai, G.
A1  - Büldt, G.
A1  - Artmann, Gerhard
T1  - From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature
JF  - Biophysical Journal. 96 (2009), H. 12
Y1  - 2009
SN  - 0006-3495
SP  - 5073
EP  - 5081
PB  - Cell Press
CY  - Cambridge, Mass.
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Unruh, Tobias
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
T1  - Cytoplasmic water and hydration layer dynamics in human red blood cells
JF  - Journal of the American Chemical Society. 50 (2008), H. 130
Y1  - 2008
SN  - 1520-5126
SP  - 16852
EP  - 16853
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Kayser, Peter
A1  - Artmann, Gerhard
T1  - Molecular processes in biological thermosensation
JF  - Journal of Biophysics. 2008 (2008)
Y1  - 2008
SN  - 1687-8000
SP  - 1
EP  - 9
ER  - 
TY  - JOUR
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Stadler, Andreas M.
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
A1  - Artmann, Gerhard
T1  - Dynamics and interactions of hemoglobin in human red blood cells and concentrated hemoglobin solutions
JF  - Regenerative medicine. 2 (2007), H. 5
Y1  - 2007
SN  - 1746-0751
N1  - Proceedings of the 3rd World Congress on Regenerative Medicine. October 18-20, 2007. Leipzig, Germany
SP  - 573
EP  - 573
ER  - 
TY  - JOUR
A1  - Maggakis-Kelemen, Christina
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Polystyrene sulfonate/Polyallylamine hydrochloride microcapsules as potential artificial red blood cells - improvement of capsule flexibility
JF  - Biomedizinische Technik. 50 (2005), H. Erg.-Bd. 1
Y1  - 2005
SP  - 324
EP  - 326
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
A1  - Artmann, Gerhard
T1  - Dynamics and interactions of hemoglobin in red blood cells
JF  - Tissue Engineering Part A. 14 (2008), H. 5
Y1  - 2008
SN  - 1937-3341
N1  - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal
SP  - 724
EP  - 724
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Linder, Peter
A1  - Porst, Dariusz
T1  - Mechanism of haemoglobin sensing body temperature
JF  - Tissue Engineering Part A. 14 (2008), H. 5
Y1  - 2008
SN  - 1937-3341
N1  - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal
SP  - 754
EP  - 754
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Stadler, Andreas M.
T1  - Physical discontinuities at body temperature in human red blood cells / Artmann, GM ; Zerlin, K ; Digel, I ; Stadler, A ; Zaccai, G ; Temiz, AA
JF  - Tissue Engineering. 13 (2007), H. 7
Y1  - 2007
SN  - 1076-3279
N1  - TERMIS-EU Meeting Abstracts London, UK September 4–7, 2007
SP  - 1778
EP  - 1778
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Zhubanova, Azhar A.
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology
JF  - Biotechnologija : teorija i praktika (2004)
Y1  - 2004
SN  - 1028-9399
SP  - 46
EP  - 52
ER  - 
TY  - CHAP
A1  - Schlemmer, Katharina
A1  - Porst, Dariusz
A1  - Bassam, Rasha
A1  - Artmann, Gerhard
A1  - Digel, Ilya
ED  - Erni, Daniel
ED  - Fischerauer, Alice
ED  - Himmel, Jörg
ED  - Seeger, Thomas
ED  - Thelen, Klaus
T1  - Effects of nitric oxide (NO) and ATP on red blood cell phenotype and deformability
T2  - 2nd YRA MedTech Symposium 2017 : June 8th - 9th / 2017 / Hochschule Ruhr-West
Y1  - 2017
SN  - 978-3-9814801-9-1
U6  - http://dx.doi.org/10.17185/duepublico/43984
N1  - A young researchers track of the 7th IEEE Workshop & SENSORICA 2017
SP  - 100
EP  - 101
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Trzewik, Jürgen
A1  - Ates, M.
T1  - A novel method to quantify mechanical tension in cell monolayers. Trzewik, Jürgen; Ates, M., Artmann, Gerhard Michael
JF  - Biomedizinische Technik. 47 (2002), H. Suppl. 1. Pt. 1
Y1  - 2002
SN  - 0013-5585
N1  - Druckausgabe unter 63 Z 47 vorhanden
SP  - 379
EP  - 381
ER  - 
TY  - JOUR
A1  - Maggakis-Kelemen, Christina
A1  - Biselli, Manfred
A1  - Artmann, Gerhard
T1  - Determination of the elastic shear modulus of cultured human red blood cells
JF  - Biomedizinische Technik. 47 (2002), H. Suppl. 1 Pt. 1
Y1  - 2002
SN  - 0013-5585
N1  - Druckausgabe unter 63 Z 471 vorhanden
SP  - 106
EP  - 109
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Zhou-Stache, J.
A1  - Buettner, R.
A1  - Mittermayer, C. [u.a.]
T1  - Inhibition of TNF-alpha induced cell death in HUVEC and Jurkat cells by protocatechuic acid. Zhou-Stache, J.; Buettner, R.; Artmann, Gerhard Michael; Mittermayer, C.; Bosserhoff, A. K.
JF  - Medical and Biological Engineering and Computing. 40 (2002), H. 6
Y1  - 2002
SN  - 0140-0118
SP  - 698
EP  - 703
ER  -