TY - JOUR A1 - Pressler, Axel A1 - Esefeld, Katrin A1 - Scherr, Johannes A1 - Ali, Mohammad A1 - Hanssen, Henner A1 - Kotliar, Konstantin A1 - Lanzl, Ines A1 - Halle, Martin A1 - Kaemmerer, Harald A1 - Schmidt-Trucksäss, Arno A1 - Hager, Alfred T1 - Structural alterations of retinal arterioles in adults late after repair of aortic isthmic coarctation JF - The American Journal of Cardiology N2 - Patients after coarctation repair still have an increased risk of cardiovascular or cerebrovascular events. This has been explained by the persisting hypertension and alterations in the peripheral vessels. However, involvement of the central vessels such as the retinal arteries is virtually unknown. A total of 34 patients after coarctation repair (22 men and 12 women; 23 to 58 years old, age range 0 to 32 years at surgical repair) and 34 nonhypertensive controls underwent structural and functional retinal vessel analysis. Using structural analysis, the vessel diameters were measured. Using functional analysis, the endothelium-dependent vessel dilation in response to flicker light stimulation was assessed. In the patients after coarctation repair, the retinal arteriolar diameter was significantly reduced compared to that of the controls (median 182 μm, first to third quartile 171 to 197; vs 197 μm, first to third quartile 193 to 206; p <0.001). These findings were independent of the peripheral blood pressure and age at intervention. No differences were found for venules. The functional analysis findings were not different between the patients and controls (maximum dilation 3.5%, first to third quartile 2.1% to 4.5% vs 3.6%, first to third quartile 2.2% to 4.3%; p = 0.81), indicating preserved autoregulative mechanisms. In conclusion, the retinal artery diameter is reduced in patients after coarctation repair, independent of their current blood pressure level and age at intervention. As a structural marker of chronic vessel damage associated with past, current, or future hypertension, retinal arteriolar narrowing has been linked to stroke incidence. These results indicate an involvement of cerebral microcirculation in aortic coarctation, despite timely repair, and might contribute to explain the increased rate of cerebrovascular events in such patients. Y1 - 2010 U6 - http://dx.doi.org/10.1016/j.amjcard.2009.10.070 SN - 0002-9149 VL - 105 IS - 5 SP - 740 EP - 744 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Wiescher, A. A1 - Kotliar, Konstantin A1 - Neuhann, T. A1 - Lanzl, Ines M. T1 - Rasch progrediente Visusminderung beider Augen bei einem jungen Patienten JF - Der Ophthalmologe N2 - Ein 34-jähriger männlicher Patient stellte sich zur Abklärung einer seit dem 9. Lebensjahr bestehenden und im letzten Jahr rasch progredienten Visusminderung beider Augen bei uns vor. Er beschrieb eine subjektiv zunehmende, im Spiegel für ihn selbst sichtbare, weißliche Trübung in der Pupille beidseits und eine starke Blendempfindlichkeit. Nebenbefundlich gab er rezidivierende Konjunktivitiden und morgens verklebte Lider an. Eine Allergie auf Gräserpollen und eine Unverträglichkeit auf Alkohol sowie mehrere Lebensmittel seien ebenfalls bekannt. Zusätzlich leidet der Patient an stark ausgeprägtem atopischem Ekzem. Dieses wurde nie systemisch, sondern nur bei Bedarf mit kortisonhaltiger Salbe therapiert. Y1 - 2010 U6 - http://dx.doi.org/10.1007/s00347-007-1586-x SN - 1433-0423 VL - 105 IS - 4 SP - 389 EP - 392 PB - Springer CY - Berlin ER - TY - JOUR A1 - Lanzl, Ines M. A1 - Seidova, Seid-Fatima A1 - Erben, A. A1 - Thürmel, K. A1 - Kotliar, Konstantin T1 - Diffuse stromale Hornhauttrübungen und Veränderungen der Hände JF - Der Ophthalmologe N2 - Bilaterale stromale Hornhauttrübungen sind für den Augenarzt eine differenzialdiagnostische Herausforderung. Im folgenden Beitrag werden 2 Patieninnen (30 und 36 Jahre) mit unterschiedlich stark ausgeprägter stromaler diffuser Hornhauttrübung vorgestellt. Patientin 1 war kleinwüchsig (114 cm) und Patientin 2 normal groß (172 cm). Beide Patientinnen wiesen veränderte Gelenkstrukturen an Hand und Fußgelenken sowie diffuse stromale Hornhauttrübungen auf. Des Weiteren lagen eine Mitral- und Aorteninsuffizienz (Patientin 1) bzw. eine Aorteninsuffizienz (Patientin 2) vor. Die stromalen diffusen Hornhauttrübungen ließen im Zusammenhang mit den Gelenkveränderungen ein Scheie-Syndrom vermuten. Therapeutisch ist bei Patienten mit Visusminderung eine (lamelläre) Keratoplastik sinnvoll. Y1 - 2010 U6 - http://dx.doi.org/10.1007/s00347-009-2066-2 SN - 1433-0423 VL - 107 IS - 4 SP - 363 EP - 365 PB - Springer CY - Berlin ER - TY - JOUR A1 - Kotliar, Konstantin A1 - Nagel, Edgar A1 - Vilser, Walthard A1 - Seidova, Seid-Fatima A1 - Lanzl, Ines T1 - Microstructural alterations of retinal arterial blood column along the vessel axis in systemic hypertension JF - Investigative Ophthalmology & Visual Science, IOVS N2 - Purpose: Image analysis by the retinal vessel analyzer (RVA) observes retinal vessels in their dynamic state online noninvasively along a chosen vessel segment. It has been found that high-frequency diameter changes in the retinal artery blood column along the vessel increase significantly in anamnestically healthy volunteers with increasing age and in patients with glaucoma during vascular dilation. This study was undertaken to investigate whether longitudinal sections of the retinal artery blood column are altered in systemic hypertension. Methods: Retinal arteries of 15 untreated patients with essential arterial hypertension (age, 50.9 ± 11.9 years) and of 15 age-matched anamnestically healthy volunteers were examined by RVA. After baseline assessment, a monochromatic luminance flicker (530–600 nm; 12.5 Hz; 20 s) was applied to evoke retinal vasodilation. Differences in amplitude and frequency of spatial artery blood column diameter change along segments (longitudinal arterial profiles) of 1 mm in length were measured and analyzed using Fourier transformation. Results: In the control group, average reduced power spectra (ARPS) of longitudinal arterial profiles did not differ when arteries changed from constriction to dilation. In the systemic hypertension group, ARPS during constriction, baseline, and restoration were identical and differed from ARPS during dilation (P < 0.05). Longitudinal arterial profiles in both groups showed significant dissimilitude at baseline and restoration (P < 0.05). Conclusions: The retinal artery blood column demonstrates microstructural alterations in systemic hypertension and is less irregular along the vessel axis during vessel dilation. These microstructural changes may be an indication of alterations in vessel wall rigidity, vascular endothelial function, and smooth muscle cells in this disease, leading to impaired perfusion and regulation. Y1 - 2010 U6 - http://dx.doi.org/10.1167/iovs.09-3649 SN - 0146-0404 VL - 51 IS - 4 SP - 2165 EP - 2172 PB - ARVO CY - Rockville, Md. ER - TY - JOUR A1 - Bogoyavlenskiy, A. P. A1 - Digel, Ilya A1 - Berezin, V. E. T1 - Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates N2 - The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine- phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI) KW - Enzyme-linked immunosorbent assay KW - Peroxidase KW - ELISA KW - phenols KW - naphtols KW - aromatic amines Y1 - 1997 ER - TY - JOUR A1 - Digel, Ilya T1 - Primary thermosensory events in cells JF - Transient receptor potential channels / Md. Shahidul Islam, ed. Y1 - 2011 SN - 978-94-007-0264-6 N1 - (Advances in experimental medicine and biology ; 704) SP - 451 EP - 468 PB - Springer CY - Dordrecht [u.a.] ER - TY - JOUR A1 - Digel, Ilya T1 - Controlling microbial adhesion : a surface engineering approach JF - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.) Y1 - 2008 SN - 978-3-540-75408-4 SP - 601 EP - 625 PB - Springer CY - Berlin [u.a.] ER - TY - JOUR A1 - Digel, Ilya A1 - Kurulgan Demirci, Eylem A1 - Linder, Peter A1 - Kayser, Peter T1 - Decrease in extracellular collagen crosslinking after NMR magnetic field application in skin fibroblasts JF - Medical and Biological Engineering and Computing. 45 (2007), H. 1 Y1 - 2007 SN - 1741-0444 SP - 91 EP - 97 ER - TY - JOUR A1 - Digel, Ilya A1 - Maggakis-Kelemen, Christina A1 - Zerlin, Kay A1 - Linder, Peter T1 - Body temperature-related structural transitions of monotremal and human hemoglobin JF - Biophysical Journal. 91 (2006), H. 8 Y1 - 2006 SN - 1542-0086 SP - 3014 EP - 3021 ER - TY - JOUR A1 - Bogoyavlenskiy, A. P. A1 - Berezin, V. E. A1 - Ogneva, A. V. A1 - Tolmacheva, V. P. A1 - Digel, Ilya A1 - Khudyakova, S. S. T1 - Immunostimulating activity of a saponin-containing extract of Saponaria officinalis JF - Voprosy virusologii Y1 - 1999 SN - 0507-4088 VL - 44 IS - 5 SP - 229 EP - 232 ER - TY - JOUR A1 - Mansurov, Z. A. A1 - Jandosov, J. M. A1 - Kerimkulova, A. R. A1 - Azat, S. A1 - Zhubanova, A. A. A1 - Digel, Ilya A1 - Savistkaya, I. S. A1 - Akimbekov, N. S. A1 - Kistaubaeva, A. S. T1 - Nanostructured carbon materials for biomedical use JF - Eurasian chemico-technological journal : quarterly journal of the International Higher Education Academy of Sciences N2 - One of the priority trends of carbon nanotechnology is creation of nanocomposite systems. Such carbon nanostructured composites were produced using - raw materials based on the products of agricultural waste, such as grape stones, apricot stones, rice husk. These products have a - wide spectrum of application and can be obtained in large quantities. The Institute of Combustion Problems has carried out the work on synthesis of the nanostructured carbon sorbents for multiple applications including the field of biomedicine. The article presents the data on the synthesis and physico-chemical properties of carbonaceous sorbents using physicochemical methods of investigation: separation and purification of biomolecules; isolation of phytohormone - fusicoccin; adsorbent INGO-1 in the form of an adsorption column for blood detoxification, oral (entero) sorbent - INGO-2; the study of efferent and probiotic properties and sorption activity in regard to the lipopolysaccharide (LPS), new biocomposites - based on carbonized rice husk (CRH) and cellular microorganisms; the use of CRH in wound treatment. A new material for blood detoxication (INGO-1) has been obtained. Adsorption of p-cresyl sulfate and indoxyl sulfate has shown that active carbon adsorbent can remove clinically significant level of p-cresyl sulfate and indoxyl sulfate from human plasma. Enterosorbent INGO-2 possesses high adsorption activity in relation to Gram-negative bacteria and their endotoxins. INGO-2 slows down the growth of conditionally pathogenic microorganisms, without having a negative effect on bifido and lactobacteria. The use of enterosorbent INGO-2 for sorption therapy may provide a solution to a complex problem - detoxication of the digestive tract and normalization of the intestinal micro ecology. The immobilized probiotic called "Riso-lact" was registered at the Ministry of Health of the Republic of Kazakhstan as a biologically active food additive. The developed technology is patented and provides production of the medicine in the form of freeze-dried biomass immobilized in vials. Y1 - 2014 U6 - http://dx.doi.org/10.18321/ectj224 SN - 1562-3920 VL - 15 (2013) IS - 3 SP - 209 EP - 217 PB - Institute of Combustion Problems CY - Almaty ER - TY - JOUR A1 - Turaliyeva, M. A1 - Yeshibaev, A. A1 - Digel, Ilya A1 - Elibayeva, G. A1 - Sydykova, A. A1 - Uspabayeva, A. A1 - Dosybayeva, G. A1 - Zhylysbayeva, A. A1 - Lakhanova, K. T1 - Molecular-genetic identification of emerged novel invasive pathogens of Asiatic Elm Ulmus pumila L JF - Life science journal N2 - The dwarf elm Ulmus pumila L. (Ulmaceae) is one of indigenous species of flora in Kazakhstan and forms a basis of dendroflora in virtually all settlements of the region. In the past decade, multiple outbreaks of previously unknown diseases of the small-leaved elm have been registered. In our study, by the molecular-genetic analysis it was found that the pathogens responsible for the outbreaks are microfungi belonging to the genus Fusarium – F. solani and F. oxysporum. The nucleotide sequences (ITS regions) isolated from the diseased trees showed very high similarity with the GenBank control numbers EU625403.1 and FJ478128.1 (100.0 and 99.0 % respectively). Oncoming research will focus on the search of natural microbial antagonists of the discovered phytopathogens. Y1 - 2014 U6 - http://dx.doi.org/10.7537/marslsj1105s14.33 SN - 1097-8135 VL - 11 IS - Spec. iss. 5s SP - 171 EP - 175 PB - Marsland Press CY - New York ER - TY - JOUR A1 - Savitskaya, I.S. A1 - Kistaubayeva, A.S. A1 - Ignatova, L.V. A1 - Digel, Ilya T1 - Antimicrobial and wound healing properties of a bacterial cellulose based material containing B. subtilis cells JF - Heliyon Y1 - 2019 U6 - http://dx.doi.org/10.1016/j.heliyon.2019.e02592 SN - 2405-8440 VL - 5 IS - 10 SP - Artikelnummer e02592 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Akimbekov, N. S. A1 - Digel, Ilya A1 - Tastambek, K. T. A1 - Zhubanova, A. A. T1 - Biocompatibility of carbonized rice husk with a rat heart cells line H9c2 JF - Experimental Biology Y1 - 2013 SN - 1563-0218 N1 - Original in russischer Sprache VL - 59 IS - 3/1 SP - 23 EP - 25 ER - TY - JOUR A1 - Akimbekov, N.Sh. A1 - Digel, Ilya A1 - Savitkaya, I.S. A1 - Zhubanova, A.A. A1 - Tastambek, K.T. T1 - Investigations of LPS endotoxin elimination in the flowing column conditions with the sorbent on the basis of carbonized rice husk JF - KazNU Bulletin. Biology series Y1 - 2013 SN - 1563-0218 N1 - Original in russischer Sprache VL - 57 IS - 1 SP - 124 EP - 127 ER - TY - JOUR A1 - Aimenova, Zh. E. A1 - Digel, Ilya A1 - Eshibaev, А. А. T1 - Dynamics of accumulation of lagochirzin in Lagochilus setulosus phytomass during the growing season and also features of its cultivation in the conditions of a typical sierozem JF - KazNU Bulletin. Biology series N2 - L.setulosus is offered for creation of biopreparation «Setulin», possesing he- mostatic action, the basic reactant of biopreparation is diterpen – lagochirzin. Results under the maintenance and dynamics of diterpen lagochirzin accumula- tion in various parts of L.setulosus are presented: in roots, stalks, leaves, flowers and calyx lobes during the growing season, and also results on conditions of cultivation L.setulosus in the conditions of a typical sierozem are resulted. From the obtained data is visible, that the given species of a plant is endemic. It is established, that dynamics of accumulation of lagochirzin in phytomass accrues from the beginning to the middle of the growing season. The chemical analysis of L.setulosus on a localization of lagochirzin in various organs of a plant, has shown, that the greatest quantity of lagochirzin collects in calyx lobes of the plants. Also it is established, that L.setulosus can be cultivated in the conditions of the typical sierozem, a mineral food is necessary for the given species of plants of Lagochilus genus, except nitric fertilizers. Comparative studying of wild-growing and cultural forms of L.setulosus has shown, that in the cultivated phytomass of plants the maintenance of lagochirzin on 17-20 % higher than in the wild-growing species. Y1 - 2016 SN - 1563-0218 N1 - Original in russischer Sprache VL - 69 IS - 4 SP - 4 EP - 11 PB - Al-Farabi Kazakh National University CY - Almaty ER - TY - JOUR A1 - Digel, Ilya A1 - Wehlitz, V. A1 - Kayser, Peter A1 - Figiel-Lange, A. A1 - Bassam, R. A1 - Rundstedt, F. von T1 - Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids JF - Plant Pathology N2 - Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach. Y1 - 2018 U6 - http://dx.doi.org/10.1111/ppa.12750 SN - 1365-3059 VL - 67 IS - 2 SP - 358 EP - 365 PB - Wiley CY - Oxford ER - TY - JOUR A1 - Mansurov, Z. A1 - Digel, Ilya A1 - Biisenbaev, M. A1 - Savistkaya, I. A1 - Kistaubaeva, A. A1 - Akimbekov, N. A1 - Zhubanova, A. T1 - Bio-composite material on the basis of carbonized rice husk in biomedicine and environmental applications JF - Eurasian Chemico-Technological Journal Y1 - 2012 U6 - http://dx.doi.org/10.18321/ectj105 SN - 2522-4867 VL - 14 IS - 2 SP - 115 EP - 131 PB - Institute of Combustion Problems CY - Almaty ER - TY - JOUR A1 - Savitskaya, I. S. A1 - Kistaubayeva, A. S. A1 - Digel, Ilya A1 - Shokatayeva, D. H. T1 - Physicochemical and Antibacterial Properties of Composite Films Based on Bacterial Cellulose and Chitosan for Wound Dressing Materials JF - Eurasian Chemico-Technological Journal Y1 - 2017 U6 - http://dx.doi.org/10.18321/ectj670 SN - 2522-4867 VL - 19 IS - 3 SP - 255 EP - 264 ER - TY - JOUR A1 - Akimbekov, N. Sh. A1 - Digel, Ilya A1 - Zhubanova, A. A. T1 - Advantages of creation of e-books in training of specialists biologists and biotechnologists JF - KazNU Bulletin. Biology series. N2 - The necessity of e-books as a primary of learning, its opportunities for realization of competence during training biologist and biotechnologist specialists are determined. Definitions and requirements to the e-books, its advantages in comparison with traditional textbooks, and the ways of creation of e-books in the SunRav BookEditor program are considered. KW - SunRav BookEditor KW - softs KW - distance learning KW - e-issues KW - e-books Y1 - 2014 SN - 1563-0218 VL - 60 IS - 1 SP - 249 EP - 252 PB - Al-Farabi Kazakh National University CY - Almaty ER -