TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
A1  - Artmann, Gerhard
T1  - Dynamics and interactions of hemoglobin in red blood cells
JF  - Tissue Engineering Part A. 14 (2008), H. 5
Y1  - 2008
SN  - 1937-3341
N1  - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal
SP  - 724
EP  - 724
ER  - 
TY  - JOUR
A1  - Stadler, A.  M.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Embs, Jan P.
A1  - Zaccai, Joe
A1  - Büldt, Georg
T1  - Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
JF  - Biophysical Journal. 95 (2008), H. 11
Y1  - 2008
SN  - 1542-0086
SP  - 5449
EP  - 5461
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Unruh, Tobias
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
T1  - Cytoplasmic water and hydration layer dynamics in human red blood cells
JF  - Journal of the American Chemical Society. 50 (2008), H. 130
Y1  - 2008
SN  - 1520-5126
SP  - 16852
EP  - 16853
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Kayser, Peter
A1  - Artmann, Gerhard
T1  - Molecular processes in biological thermosensation
JF  - Journal of Biophysics. 2008 (2008)
Y1  - 2008
SN  - 1687-8000
SP  - 1
EP  - 9
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Garvey, G. J.
A1  - Bocahut, A.
A1  - Sacquin-Mora, S.
A1  - Digel, Ilya
A1  - Schneider, G. J.
A1  - Natali, F.
A1  - Artmann, Gerhard
A1  - Zaccai, G.
T1  - Thermal fluctuations of haemoglobin from different species : adaptation to temperature via conformational dynamics
JF  - Journal of the Royal Society Interface
N2  - Thermodynamic stability, configurational motions and internal forces of haemoglobin (Hb) of three endotherms (platypus, Ornithorhynchus anatinus; domestic chicken, Gallus gallus domesticus and human, Homo sapiens) and an ectotherm (salt water crocodile, Crocodylus porosus) were investigated using circular dichroism, incoherent elastic neutron scattering and coarse-grained Brownian dynamics simulations. The experimental results from Hb solutions revealed a direct correlation between protein resilience, melting temperature and average body temperature of the different species on the 0.1 ns time scale. Molecular forces appeared to be adapted to permit conformational fluctuations with a root mean square displacement close to 1.2 Å at the corresponding average body temperature of the endotherms. Strong forces within crocodile Hb maintain the amplitudes of motion within a narrow limit over the entire temperature range in which the animal lives. In fully hydrated powder samples of human and chicken, Hb mean square displacements and effective force constants on the 1 ns time scale showed no differences over the whole temperature range from 10 to 300 K, in contrast to the solution case. A complementary result of the study, therefore, is that one hydration layer is not sufficient to activate all conformational fluctuations of Hb in the pico- to nanosecond time scale which might be relevant for biological function. Coarse-grained Brownian dynamics simulations permitted to explore residue-specific effects. They indicated that temperature sensing of human and chicken Hb occurs mainly at residues lining internal cavities in the β-subunits.
Y1  - 2012
U6  - https://doi.org/10.1098/rsif.2012.0364
SN  - 1742-5689
VL  - 9
IS  - 76
SP  - 2845
EP  - 2855
PB  - The Royal Society
CY  - London
ER  - 
TY  - JOUR
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Stadler, Andreas M.
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
A1  - Artmann, Gerhard
T1  - Dynamics and interactions of hemoglobin in human red blood cells and concentrated hemoglobin solutions
JF  - Regenerative medicine. 2 (2007), H. 5
Y1  - 2007
SN  - 1746-0751
N1  - Proceedings of the 3rd World Congress on Regenerative Medicine. October 18-20, 2007. Leipzig, Germany
SP  - 573
EP  - 573
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Zhubanova, Azhar A.
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology
JF  - Biotechnologija : teorija i praktika (2004)
Y1  - 2004
SN  - 1028-9399
SP  - 46
EP  - 52
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Stadler, Andreas M.
T1  - Physical discontinuities at body temperature in human red blood cells / Artmann, GM ; Zerlin, K ; Digel, I ; Stadler, A ; Zaccai, G ; Temiz, AA
JF  - Tissue Engineering. 13 (2007), H. 7
Y1  - 2007
SN  - 1076-3279
N1  - TERMIS-EU Meeting Abstracts London, UK September 4–7, 2007
SP  - 1778
EP  - 1778
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Linder, Peter
A1  - Porst, Dariusz
T1  - Mechanism of haemoglobin sensing body temperature
JF  - Tissue Engineering Part A. 14 (2008), H. 5
Y1  - 2008
SN  - 1937-3341
N1  - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal
SP  - 754
EP  - 754
ER  - 
TY  - JOUR
A1  - Maggakis-Kelemen, Christina
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Polystyrene sulfonate/Polyallylamine hydrochloride microcapsules as potential artificial red blood cells - improvement of capsule flexibility
JF  - Biomedizinische Technik. 50 (2005), H. Erg.-Bd. 1
Y1  - 2005
SP  - 324
EP  - 326
ER  - 
TY  - JOUR
A1  - Konstantinidis, Konstantinos
A1  - Flores Martinez, Claudio
A1  - Dachwald, Bernd
A1  - Ohndorf, Andreas
A1  - Dykta, Paul
A1  - Bowitz, Pascal
A1  - Rudolph, Martin
A1  - Digel, Ilya
A1  - Kowalski, Julia
A1  - Voigt, Konstantin
A1  - Förstner, Roger
T1  - A lander mission to probe subglacial water on Saturn's moon enceladus for life
JF  - Acta astronautica
Y1  - 2015
SN  - 1879-2030 (E-Journal); 0094-5765 (Print)
VL  - Vol. 106
SP  - 63
EP  - 89
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Dachwald, Bernd
A1  - Mikucki, Jill
A1  - Tulaczyk, Slawek
A1  - Digel, Ilya
A1  - Espe, Clemens
A1  - Feldmann, Marco
A1  - Francke, Gero
A1  - Kowalski, Julia
A1  - Xu, Changsheng
T1  - IceMole : A maneuverable probe for clean in situ analysis and sampling of subsurface ice and subglacial aquatic ecosystems
JF  - Annals of Glaciology
N2  - There is significant interest in sampling subglacial environments for geobiological studies, but they are difficult to access. Existing ice-drilling technologies make it cumbersome to maintain microbiologically clean access for sample acquisition and environmental stewardship of potentially fragile subglacial aquatic ecosystems. The IceMole is a maneuverable subsurface ice probe for clean in situ analysis and sampling of glacial ice and subglacial materials. The design is based on the novel concept of combining melting and mechanical propulsion. It can change melting direction by differential heating of the melting head and optional side-wall heaters. The first two prototypes were successfully tested between 2010 and 2012 on glaciers in Switzerland and Iceland. They demonstrated downward, horizontal and upward melting, as well as curve driving and dirt layer penetration. A more advanced probe is currently under development as part of the Enceladus Explorer (EnEx) project. It offers systems for obstacle avoidance, target detection, and navigation in ice. For the EnEx-IceMole, we will pay particular attention to clean protocols for the sampling of subglacial materials for biogeochemical analysis. We plan to use this probe for clean access into a unique subglacial aquatic environment at Blood Falls, Antarctica, with return of a subglacial brine sample.
KW  - Antarctic Glaciology
KW  - Extraterrestrial Glaciology
KW  - Glaciological instruments and methods
KW  - Subclacial exploration
KW  - Subglacial lakes
Y1  - 2014
U6  - https://doi.org/10.3189/2014AoG65A004
SN  - 1727-5644
VL  - 55
IS  - 65
SP  - 14
EP  - 22
PB  - Cambridge University Press
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Mikucki, Jill Ann
A1  - Schuler, C. G.
A1  - Digel, Ilya
A1  - Kowalski, Julia
A1  - Tuttle, M. J.
A1  - Chua, Michelle
A1  - Davis, R.
A1  - Purcell, Alicia
A1  - Ghosh, D.
A1  - Francke, G.
A1  - Feldmann, Marco
A1  - Espe, C.
A1  - Heinen, Dirk
A1  - Dachwald, Bernd
A1  - Clemens, Joachim
A1  - Lyons, W. B.
A1  - Tulaczyk, S.
T1  - Field-Based planetary protection operations for melt probes: validation of clean access into the blood falls, antarctica, englacial ecosystem
JF  - Astrobiology
N2  - Subglacial environments on Earth offer important analogs to Ocean World targets in our solar system. These unique microbial ecosystems remain understudied due to the challenges of access through thick glacial ice (tens to hundreds of meters). Additionally, sub-ice collections must be conducted in a clean manner to ensure sample integrity for downstream microbiological and geochemical analyses. We describe the field-based cleaning of a melt probe that was used to collect brine samples from within a glacier conduit at Blood Falls, Antarctica, for geomicrobiological studies. We used a thermoelectric melting probe called the IceMole that was designed to be minimally invasive in that the logistical requirements in support of drilling operations were small and the probe could be cleaned, even in a remote field setting, so as to minimize potential contamination. In our study, the exterior bioburden on the IceMole was reduced to levels measured in most clean rooms, and below that of the ice surrounding our sampling target. Potential microbial contaminants were identified during the cleaning process; however, very few were detected in the final englacial sample collected with the IceMole and were present in extremely low abundances (∼0.063% of 16S rRNA gene amplicon sequences). This cleaning protocol can help minimize contamination when working in remote field locations, support microbiological sampling of terrestrial subglacial environments using melting probes, and help inform planetary protection challenges for Ocean World analog mission concepts.
Y1  - 2023
U6  - https://doi.org/10.1089/ast.2021.0102
SN  - 1557-8070 (online)
SN  - 153-1074 (print)
VL  - 23
IS  - 11
SP  - 1165
EP  - 1178
PB  - Liebert
CY  - New York, NY
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
T1  - Analytische Berechnung von Gummi- Metall- Verbindungen
JF  - Konstruktion : Zeitschrift für Produktentwicklung. 39 (1987), H. 4
Y1  - 1987
SN  - 0373-3300
SP  - 151
EP  - 158
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Lutz, Th.
T1  - Ein Beitrag zur Bestimmung der Haertewerte von Elastomeren
JF  - Kautschuk Gummi Kunststoffe. 43 (1990), H. 10
Y1  - 1990
SN  - 0022-9520
SP  - 861
EP  - 865
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Akbay, U.
T1  - Über den Mechanismus der Oberflächeninstabilität viskosimetrischer Strömungen
JF  - Rheologica Acta. 23 (1984), H. 2
Y1  - 1984
SN  - 0035-4511
SP  - 185
EP  - 188
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Akbay, U.
A1  - Becker, E.
A1  - Krozer, S.
T1  - Instability of slow viscometric flow
JF  - Mechanics Research Communications. 7 (1980), H. 4
Y1  - 1980
SN  - 0093-6413
SP  - 199
EP  - 204
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Adbay, U.
A1  - Becker, E.
A1  - Krozer, S.
T1  - A possible cause of viscoelastic turbulence
JF  - Rheology : proceedings of the 8th International Congress of Rheology, held in Naples, Italy, September 1 - 5, 1980 / Hrsg. von Giovanni Astarita, Vol. 2
Y1  - 1980
SN  - 0-306-40465-6
SP  - 79
EP  - 84
PB  - Plenum Pr.
CY  - New York
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Akbay, S.
T1  - Über die Stabilität viskometrischer Strömungen
JF  - Rheologica acta. 20 (1981), H. 6
Y1  - 1981
SN  - 0035-4511
SP  - 579
EP  - 590
ER  - 
TY  - JOUR
A1  - Sponagel, Stefan
A1  - Akbay, U.
A1  - Becker, E.
T1  - Instability of plane Couette flow of viscoelastic liquids
JF  - Journal of non-Newtonian fluid mechanics. 18 (1985), H. 2
Y1  - 1985
SN  - 0377-0257
SP  - 123
EP  - 141
ER  -