TY - JOUR A1 - Micili, Serap C. A1 - Valter, Markus A1 - Oflaz, Hakan A1 - Ozogul, Candan A1 - Linder, Peter A1 - Föckler, Nicole A1 - Artmann, Gerhard A1 - Digel, Ilya A1 - Temiz Artmann, Aysegül T1 - Optical coherence tomography : a potential tool to predict premature rupture of fetal membranes JF - Proceedings of the Institution of Mechanical Engineers. Part H : Journal of engineering in medicine Y1 - 2013 SN - 0046-2039 (Print) ; 2041-3033 (E-Journal) VL - Vol. 227 IS - No. 4 SP - 393 EP - 401 PB - Sage CY - London ER - TY - JOUR A1 - Kurz, R. A1 - Linder, Peter A1 - Trzewik, Jürgen A1 - Rüffer, M. A1 - Artmann, Gerhard A1 - Digel, Ilya A1 - Rothermel, A. A1 - Robitzki, A. A1 - Temiz Artmann, Aysegül T1 - Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes JF - Medical and Biological Engineering and Computing N2 - The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching. Y1 - 2010 U6 - http://dx.doi.org/10.1007/s11517-009-0552-y SN - 1741-0444 VL - 48 IS - 1 SP - 59 EP - 65 PB - Springer Nature CY - Cham ER - TY - JOUR A1 - Goßmann, Matthias A1 - Frotscher, Ralf A1 - Linder, Peter A1 - Bayer, Robin A1 - Epple, U. A1 - Staat, Manfred A1 - Temiz Artmann, Aysegül A1 - Artmann, Gerhard T1 - Mechano-pharmacological characterization of cardiomyocytes derived from human induced pluripotent stem cells JF - Cellular physiology and biochemistry N2 - Background/Aims: Common systems for the quantification of cellular contraction rely on animal-based models, complex experimental setups or indirect approaches. The herein presented CellDrum technology for testing mechanical tension of cellular monolayers and thin tissue constructs has the potential to scale-up mechanical testing towards medium-throughput analyses. Using hiPS-Cardiac Myocytes (hiPS-CMs) it represents a new perspective of drug testing and brings us closer to personalized drug medication. Methods: In the present study, monolayers of self-beating hiPS-CMs were grown on ultra-thin circular silicone membranes and deflect under the weight of the culture medium. Rhythmic contractions of the hiPS-CMs induced variations of the membrane deflection. The recorded contraction-relaxation-cycles were analyzed with respect to their amplitudes, durations, time integrals and frequencies. Besides unstimulated force and tensile stress, we investigated the effects of agonists and antagonists acting on Ca²⁺ channels (S-Bay K8644/verapamil) and Na⁺ channels (veratridine/lidocaine). Results: The measured data and simulations for pharmacologically unstimulated contraction resembled findings in native human heart tissue, while the pharmacological dose-response curves were highly accurate and consistent with reference data. Conclusion: We conclude that the combination of the CellDrum with hiPS-CMs offers a fast, facile and precise system for pharmacological, toxicological studies and offers new preclinical basic research potential. KW - Inotropic compounds KW - Pharmacology KW - Ion channels KW - CellDrum KW - Heart tissue culture KW - Induced pluripotent stem cells KW - Cardiac myocytes Y1 - 2016 U6 - http://dx.doi.org/10.1159/000443124 SN - 1421-9778 (Online) SN - 1015-8987 (Print) VL - 38 IS - 3 SP - 1182 EP - 1198 PB - Karger CY - Basel ER - TY - CHAP A1 - Kurulgan Demirci, Eylem A1 - Linder, Peter A1 - Demirci, Taylan A1 - Gierkowski, Jessica R. A1 - Digel, Ilya A1 - Gossmann, Matthias A1 - Temiz Artmann, Aysegül T1 - rhAPC reduces the endothelial cell permeability via a decrease of cellular mechanical contractile tensions : [abstract] N2 - In this study, the CellDrum technology quanitfying cellular mechanical tension on a pico-scale was used to investigate the effect of LPS (lipopolysaccharide) on HAoEC (Human Aortic Endothelial Cell) tension. KW - Endothelzelle KW - Sepsis KW - kontraktile Spannung KW - rhAPC KW - contractile tension KW - rhAPC KW - celldrum technology Y1 - 2010 ER - TY - CHAP A1 - Digel, Ilya A1 - Demirci, Taylan A1 - Trzewik, Jürgen A1 - Linder, Peter A1 - Temiz Artmann, Aysegül T1 - Fibroblast response to mechanical stress: role of the adhesion substrate : [abstract] N2 - Mechanical stimulation of the cells resulted in evident changes in the cell morphology, protein composition and gene expression. Microscopically, additional formation of stress fibers accompanied by cell re-arrangements in a monolayer was observed. Also, significant activation of p53 gene was revealed as compared to control. Interestingly, the use of CellTech membrane coating induced cell death after mechanical stress had been applied. Such an effect was not detected when fibronectin had been used as an adhesion substrate. KW - Fibroblast KW - Mechanische Beanspruchung KW - celldrum technology Y1 - 2004 ER - TY - JOUR A1 - Temiz Artmann, Aysegül A1 - Linder, Peter A1 - Kayser, Peter A1 - Digel, Ilya T1 - NMR in vitro effects on proliferation, apoptosis, and viability of human chondrocytes and osteoblasts JF - Methods and findings in Experimental and Clinical Pharmacology. 27 (2005), H. 6 Y1 - 2005 SN - 0379-0355 SP - 391 EP - 394 ER - TY - CHAP A1 - Digel, Ilya A1 - Dachwald, Bernd A1 - Artmann, Gerhard A1 - Linder, Peter A1 - Funke, O. T1 - A concept of a probe for particle analysis and life detection in icy environments N2 - A melting probe equipped with autofluorescence-based detection system combined with a light scattering unit, and, optionally, with a microarray chip would be ideally suited to probe icy environments like Europa’s ice layer as well as the polar ice layers of Earth and Mars for recent and extinct live. KW - Sonde KW - Eisschicht KW - Autofluoreszenzverfahren KW - Lichtstreuungsbasierte Instrumente KW - autofluorescence-based detection system KW - light scattering analysis Y1 - 2009 ER - TY - JOUR A1 - Digel, Ilya A1 - Dachwald, Bernd A1 - Artmann, Gerhard A1 - Linder, Peter A1 - Funke, O. T1 - A concept of a probe for particle analysis and life detection in icy environments Y1 - 2009 N1 - International workshop “Europa lander: science goals and experiments”, Space Research Institute (IKI), Moscow, Russia 9-13 February 2009 SP - 1 EP - 24 ER - TY - JOUR A1 - Kowalski, Julia A1 - Linder, Peter A1 - Zierke, S. A1 - Wulfen, B. van A1 - Clemens, J. A1 - Konstantinidis, K. A1 - Ameres, G. A1 - Hoffmann, R. A1 - Mikucki, J. A1 - Tulaczyk, S. A1 - Funke, O. A1 - Blandfort, D. A1 - Espe, Clemens A1 - Feldmann, Marco A1 - Francke, Gero A1 - Hiecker, S. A1 - Plescher, Engelbert A1 - Schöngarth, Sarah A1 - Dachwald, Bernd A1 - Digel, Ilya A1 - Artmann, Gerhard A1 - Eliseev, D. A1 - Heinen, D. A1 - Scholz, F. A1 - Wiebusch, C. A1 - Macht, S. A1 - Bestmann, U. A1 - Reineking, T. A1 - Zetzsche, C. A1 - Schill, K. A1 - Förstner, R. A1 - Niedermeier, H. A1 - Szumski, A. A1 - Eissfeller, B. A1 - Naumann, U. A1 - Helbing, K. T1 - Navigation technology for exploration of glacier ice with maneuverable melting probes JF - Cold Regions Science and Technology N2 - The Saturnian moon Enceladus with its extensive water bodies underneath a thick ice sheet cover is a potential candidate for extraterrestrial life. Direct exploration of such extraterrestrial aquatic ecosystems requires advanced access and sampling technologies with a high level of autonomy. A new technological approach has been developed as part of the collaborative research project Enceladus Explorer (EnEx). The concept is based upon a minimally invasive melting probe called the IceMole. The force-regulated, heater-controlled IceMole is able to travel along a curved trajectory as well as upwards. Hence, it allows maneuvers which may be necessary for obstacle avoidance or target selection. Maneuverability, however, necessitates a sophisticated on-board navigation system capable of autonomous operations. The development of such a navigational system has been the focal part of the EnEx project. The original IceMole has been further developed to include relative positioning based on in-ice attitude determination, acoustic positioning, ultrasonic obstacle and target detection integrated through a high-level sensor fusion. This paper describes the EnEx technology and discusses implications for an actual extraterrestrial mission concept. Y1 - 2016 U6 - http://dx.doi.org/10.1016/j.coldregions.2015.11.006 SN - 0165-232X IS - 123 SP - 53 EP - 70 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Demirci, T. A1 - Trzewik, J. A1 - Linder, Peter A1 - Artmann, Gerhard A1 - Temiz Artmann, Aysegül T1 - Mechanical Stimulation of 3T3 Fibroblasts Activates Genes: Real Time PCR Products and Suppliers by Comparison JF - Biomedizinische Technik . 49 (2004), H. Erg.-Bd. 2 Y1 - 2004 SN - 0932-4666 SP - 1046 EP - 1047 ER -