TY  - JOUR
A1  - Bayer, Robin
A1  - Temiz Artmann, Aysegül
A1  - Digel, Ilya
A1  - Falkenstein, Julia
A1  - Artmann, Gerhard
A1  - Creutz, Till
A1  - Hescheler, Jürgen
T1  - Mechano-pharmacological testing of L-Type Ca²⁺ channel modulators via human vascular celldrum model
JF  - Cellular Physiology and Biochemistry
N2  - Background/Aims: This study aimed to establish a precise and well-defined working model, assessing pharmaceutical effects on vascular smooth muscle cell monolayer in-vitro. It describes various analysis techniques to determine the most suitable to measure the biomechanical impact of vasoactive agents by using CellDrum technology. Methods: The so-called CellDrum technology was applied to analyse the biomechanical properties of confluent human aorta muscle cells (haSMC) in monolayer. The cell generated tensions deviations in the range of a few N/m² are evaluated by the CellDrum technology. This study focuses on the dilative and contractive effects of L-type Ca²⁺ channel agonists and antagonists, respectively. We analyzed the effects of Bay K8644, nifedipine and verapamil. Three different measurement modes were developed and applied to determine the most appropriate analysis technique for the study purpose. These three operation modes are called, particular time mode" (PTM), "long term mode" (LTM) and "real-time mode" (RTM). Results: It was possible to quantify the biomechanical response of haSMCs to the addition of vasoactive agents using CellDrum technology. Due to the supplementation of 100nM Bay K8644, the tension increased approximately 10.6% from initial tension maximum, whereas, the treatment with nifedipine and verapamil caused a significant decrease in cellular tension: 10nM nifedipine decreased the biomechanical stress around 6,5% and 50nM verapamil by 2,8%, compared to the initial tension maximum. Additionally, all tested measurement modes provide similar results while focusing on different analysis parameters. Conclusion: The CellDrum technology allows highly sensitive biomechanical stress measurements of cultured haSMC monolayers. The mechanical stress responses evoked by the application of vasoactive calcium channel modulators were quantified functionally (N/m²). All tested operation modes resulted in equal findings, whereas each mode features operation-related data analysis.
Y1  - 2020
U6  - http://dx.doi.org/10.33594/000000225
SN  - 1421-9778
VL  - 54
SP  - 371
EP  - 383
PB  - Cell Physiol Biochem Press
CY  - Düsseldorf
ER  - 
TY  - JOUR
A1  - Akimbekov, N. Sh.
A1  - Zhubanova, A. A.
A1  - Mansurov, Z. A.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Use of Carbonized Rise Shell for the local treatment of wounds
JF  - Eurasian ChemTech Journal
N2  - On the model of musculocutaneous wound in rats, the effect of applicative sorption by carbonized rise shell (CRS) on the healing of festering wound was studied. It has been shown, that cytological changes end with rapid scar formation. The use of CRS at the period of severe purulent wound contributes to its favorable course, prevents the development of complications of the animals from sepsis.
Y1  - 2010
U6  - http://dx.doi.org/10.18321/ectj35
SN  - 2522-4867
VL  - 12
IS  - 2
SP  - 133
EP  - 138
PB  - Institute of Combustion Problems
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Kozhalakova, A. A.
A1  - Zhubanova, Azhar A.
A1  - Mansurov, Z. A.
A1  - Digel, Ilya
A1  - Tazhibayeva, S. M.
A1  - Artmann, Gerhard
A1  - Temiz Artmann, Aysegül
T1  - Adsorption of bacterial lipopolysaccharides on carbonized rice shell
JF  - Science of Central Asia (2010)
Y1  - 2010
SP  - 50
EP  - 54
ER  - 
TY  - JOUR
A1  - Kurz, R.
A1  - Linder, Peter
A1  - Trzewik, Jürgen
A1  - Rüffer, M.
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Rothermel, A.
A1  - Robitzki, A.
A1  - Temiz Artmann, Aysegül
T1  - Contractile tension and beating rates of self-exciting monolayers and 3D-tissue constructs of neonatal rat cardiomyocytes
JF  - Medical and Biological Engineering and Computing
N2  - The CellDrum technology (The term 'CellDrum technology' includes a couple of slightly different technological setups for measuring lateral mechanical tension in various types of cell monolayers or 3D-tissue constructs) was designed to quantify the contraction rate and mechanical tension of self-exciting cardiac myocytes. Cells were grown either within flexible, circular collagen gels or as monolayer on top of respective 1-mum thin silicone membranes. Membrane and cells were bulged outwards by air pressure. This biaxial strain distribution is rather similar the beating, blood-filled heart. The setup allowed presetting the mechanical residual stress level externally by adjusting the centre deflection, thus, mimicking hypertension in vitro. Tension was measured as oscillating differential pressure change between chamber and environment. A 0.5-mm thick collagen-cardiac myocyte tissue construct induced after 2 days of culturing (initial cell density 2 x 10(4) cells/ml), a mechanical tension of 1.62 +/- 0.17 microN/mm(2). Mechanical load is an important growth regulator in the developing heart, and the orientation and alignment of cardiomyocytes is stress sensitive. Therefore, it was necessary to develop the CellDrum technology with its biaxial stress-strain distribution and defined mechanical boundary conditions. Cells were exposed to strain in two directions, radially and circumferentially, which is similar to biaxial loading in real heart tissues. Thus, from a biomechanical point of view, the system is preferable to previous setups based on uniaxial stretching.
Y1  - 2010
U6  - http://dx.doi.org/10.1007/s11517-009-0552-y
SN  - 1741-0444
VL  - 48
IS  - 1
SP  - 59
EP  - 65
PB  - Springer Nature
CY  - Cham
ER  - 
TY  - CHAP
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Demirci, Taylan
A1  - Gierkowski, Jessica R.
A1  - Digel, Ilya
A1  - Gossmann, Matthias
A1  - Temiz Artmann, Aysegül
T1  - rhAPC reduces the endothelial cell permeability via a decrease of cellular mechanical contractile tensions : [abstract]
N2  - In this study, the CellDrum technology quanitfying cellular mechanical tension on a pico-scale was used to investigate the effect of LPS (lipopolysaccharide) on HAoEC (Human Aortic Endothelial Cell) tension.
KW  - Endothelzelle
KW  - Sepsis
KW  - kontraktile Spannung
KW  - rhAPC
KW  - contractile tension
KW  - rhAPC
KW  - celldrum technology
Y1  - 2010
ER  - 
TY  - CHAP
A1  - O\'Heras, Carlos
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - Nanostructured carbon-based column for LPS/protein adsorption : [abstract]
N2  - The absence of a general method for endotoxin removal from liquid interfaces gives an opportunity to find new methods and materials to overcome this gap. Activated nanostructured carbon is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study is to find the adsorption rates for a carboneous material produced at different temperatures, as well as to reveal possible differences between the performance of the material for each of the adsorbates used during the study (hemoglobin, serum albumin and lipopolysaccharide, LPS).
KW  - Kohlenstofffaser
KW  - Adsorption
KW  - Lipopolysaccharide
KW  - aktivierte nanostrukturierte Kohlenstofffaser
KW  - lipopolysaccharides
KW  - activated nanostructured carbon
Y1  - 2009
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Demirci, Taylan
A1  - Trzewik, Jürgen
A1  - Linder, Peter
A1  - Temiz Artmann, Aysegül
T1  - Fibroblast response to mechanical stress: role of the adhesion substrate : [abstract]
N2  - Mechanical stimulation of the cells resulted in evident changes in the cell morphology, protein composition and gene expression. Microscopically, additional formation of stress fibers accompanied by cell re-arrangements in a monolayer was observed. Also, significant activation of p53 gene was revealed as compared to control. Interestingly, the use of CellTech membrane coating induced cell death after mechanical stress had been applied. Such an effect was not detected when fibronectin had been used as an adhesion substrate.
KW  - Fibroblast
KW  - Mechanische Beanspruchung
KW  - celldrum technology
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Temiz Artmann, Aysegül
A1  - Linder, Peter
A1  - Kayser, Peter
A1  - Digel, Ilya
T1  - NMR in vitro effects on proliferation, apoptosis, and viability of human chondrocytes and osteoblasts
JF  - Methods and findings in Experimental and Clinical Pharmacology. 27 (2005), H. 6
Y1  - 2005
SN  - 0379-0355
SP  - 391
EP  - 394
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
T1  - The emperor's new body : seeking for a blueprint of limb regeneration in humans
JF  - Stem cell engineering : principles and applications / Gerhard M. Artmann ... eds.
Y1  - 2011
SN  - 978-3-642-11864-7
SP  - 3
EP  - 37
PB  - Springer
CY  - Berlin [u.a.]
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Zerlin, Kay
A1  - Temiz Artmann, Aysegül
A1  - Engels, S.
T1  - Protein dynamics in thermosensation
JF  - Regenerative medicine. 2 (2007), H. 5
Y1  - 2007
SN  - 1746-0751
N1  - Proceedings of the 3rd World Congress on Regenerative Medicine. October 18-20, 2007. Leipzig, Germany
SP  - 533
EP  - 533
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Temiz Artmann, Aysegül
A1  - Nishikawa, K.
A1  - Cook, M.
T1  - Bactericidal effects of plasma-generated cluster ions
JF  - Medical and Biological Engineering and Computing. 43 (2005), H. 6
Y1  - 2005
SN  - 1741-0444
SP  - 800
EP  - 807
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Trzewik, Jürgen
A1  - Demirci, Taylan
A1  - Temiz Artmann, Aysegül
T1  - Response of fibroblasts to cyclic mechanical stress : a proteome approach / Digel, I. ; Trzewik, J. ; Demirci, T. ; Temiz Artmann, A. ; Artmann, G. M.
JF  - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2
Y1  - 2004
SN  - 0932-4666
SP  - 1042
EP  - 1043
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Demirci, Taylan
A1  - Temiz Artmann, Aysegül
A1  - Nishikawa, K.
T1  - Free Radical Nature of the Bactericidal Effect of Plasma-Generated Cluster Ions (PCIs)
JF  - Biomedizinische Technik. 49 (2004), H. Erg.-Bd. 2
Y1  - 2004
SN  - 0932-4666
SP  - 982
EP  - 983
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Akimbekov, N.
A1  - Turalieva, M.
A1  - Mansurov, Z.
A1  - Temiz Artmann, Aysegül
A1  - Eshibaev, A.
A1  - Zhubanova, A.
T1  - Usage of Carbonized Plant Wastes for Purification of Aqueous Solutions
JF  - Journal of Industrial Technology and Engineering
Y1  - 2013
VL  - 2
IS  - 07
SP  - 47
EP  - 54
ER  - 
TY  - CHAP
A1  - Dachwald, Bernd
A1  - Xu, Changsheng
A1  - Feldmann, Marco
A1  - Plescher, Engelbert
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Development and testing of a subsurface probe for detection of life in deep ice : [abstract]
N2  - We present the novel concept of a combined drilling and melting probe for subsurface ice research. This probe, named “IceMole”, is currently developed, built, and tested at the FH Aachen University of Applied Sciences’ Astronautical Laboratory. Here, we describe its first prototype design and report the results of its field tests on the Swiss Morteratsch glacier. Although the IceMole design is currently adapted to terrestrial glaciers and ice shields, it may later be modified for the subsurface in-situ investigation of extraterrestrial ice, e.g., on Mars, Europa, and Enceladus. If life exists on those bodies, it may be present in the ice (as life can also be found in the deep ice of Earth).
KW  - Eisschicht
KW  - Sonde
KW  - subsurface probe
KW  - subsurface ice research
Y1  - 2011
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Dachwald, Bernd
A1  - Artmann, Gerhard
A1  - Linder, Peter
A1  - Funke, O.
T1  - A concept of a probe for particle analysis and life detection in icy environments
N2  - A melting probe equipped with autofluorescence-based detection system combined with a light scattering unit, and, optionally, with a microarray chip would be ideally suited to probe icy environments like Europa’s ice layer as well as the polar ice layers of Earth and Mars for recent and extinct live.
KW  - Sonde
KW  - Eisschicht
KW  - Autofluoreszenzverfahren
KW  - Lichtstreuungsbasierte Instrumente
KW  - autofluorescence-based detection system
KW  - light scattering analysis
Y1  - 2009
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Dachwald, Bernd
A1  - Artmann, Gerhard
A1  - Linder, Peter
A1  - Funke, O.
T1  - A concept of a probe for particle analysis and life detection in icy environments
Y1  - 2009
N1  - International workshop “Europa lander: science goals and experiments”, Space Research Institute (IKI), Moscow, Russia
9-13 February 2009
SP  - 1
EP  - 24
ER  - 
TY  - JOUR
A1  - Kowalski, Julia
A1  - Linder, Peter
A1  - Zierke, S.
A1  - Wulfen, B. van
A1  - Clemens, J.
A1  - Konstantinidis, K.
A1  - Ameres, G.
A1  - Hoffmann, R.
A1  - Mikucki, J.
A1  - Tulaczyk, S.
A1  - Funke, O.
A1  - Blandfort, D.
A1  - Espe, Clemens
A1  - Feldmann, Marco
A1  - Francke, Gero
A1  - Hiecker, S.
A1  - Plescher, Engelbert
A1  - Schöngarth, Sarah
A1  - Dachwald, Bernd
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Eliseev, D.
A1  - Heinen, D.
A1  - Scholz, F.
A1  - Wiebusch, C.
A1  - Macht, S.
A1  - Bestmann, U.
A1  - Reineking, T.
A1  - Zetzsche, C.
A1  - Schill, K.
A1  - Förstner, R.
A1  - Niedermeier, H.
A1  - Szumski, A.
A1  - Eissfeller, B.
A1  - Naumann, U.
A1  - Helbing, K.
T1  - Navigation technology for exploration of glacier ice with maneuverable melting probes
JF  - Cold Regions Science and Technology
N2  - The Saturnian moon Enceladus with its extensive water bodies underneath a thick ice sheet cover is a potential candidate for extraterrestrial life. Direct exploration of such extraterrestrial aquatic ecosystems requires advanced access and sampling technologies with a high level of autonomy. A new technological approach has been developed as part of the collaborative research project Enceladus Explorer (EnEx). The concept is based upon a minimally invasive melting probe called the IceMole. The force-regulated, heater-controlled IceMole is able to travel along a curved trajectory as well as upwards. Hence, it allows maneuvers which may be necessary for obstacle avoidance or target selection. Maneuverability, however, necessitates a sophisticated on-board navigation system capable of autonomous operations. The development of such a navigational system has been the focal part of the EnEx project. The original IceMole has been further developed to include relative positioning based on in-ice attitude determination, acoustic positioning, ultrasonic obstacle and target detection integrated through a high-level sensor fusion. This paper describes the EnEx technology and discusses implications for an actual extraterrestrial mission concept.
Y1  - 2016
U6  - http://dx.doi.org/10.1016/j.coldregions.2015.11.006
SN  - 0165-232X
IS  - 123
SP  - 53
EP  - 70
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - CHAP
A1  - Dachwald, Bernd
A1  - Mikucki, Jill A.
A1  - Tulaczyk, Slawek
A1  - Digel, Ilya
A1  - Feldmann, Marco
A1  - Espe, Clemens
A1  - Plescher, Engelbert
A1  - Xu, Changsheng
T1  - IceMole - a maneuverable probe for clean in-situ analysis and sampling of subsurface ice and subglacial aquatic ecosystems : extended abstract / SCAR Open Science Conference 2012, Session 29: Advancing Clean Technologies for Exploration of Glacial Aquatic Ecosystems
N2  - The ”IceMole“ is a novel maneuverable subsurface ice probe for clean in-situ analysis and sampling of subsurface ice and subglacial water/brine. It is developed and build at FH Aachen University of Applied Sciences’ Astronautical Laboratory. A first prototype was successfully tested on the Swiss Morteratsch glacier in 2010. Clean sampling is achieved with a hollow ice screw (as it is used in mountaineering) at the tip of the probe. Maneuverability is achieved with a differentially heated melting head. Funded by the German Space Agency (DLR), a consortium led by FH Aachen currently develops a much more advanced IceMole probe, which includes a sophisticated system for obstacle avoidance, target detection, and navigation in the ice. We intend to use this probe for taking clean samples of subglacial brine at the Blood Falls (McMurdo Dry Valleys, East Antarctica) for chemical and microbiological analysis. In our conference contribution, we 1) describe the IceMole design, 2) report the results of the field tests of the first prototype on the Morteratsch glacier, 3) discuss the probe’s potential for the clean in-situ analysis and sampling of subsurface ice and subglacial liquids, and 4) outline the way ahead in the development of this technology.
KW  - Eisschicht
KW  - Sonde
KW  - subsurface ice
KW  - subglacial aquatic ecosystems
Y1  - 2012
ER  - 
TY  - JOUR
A1  - Konstantinidis, Konstantinos
A1  - Flores Martinez, Claudio
A1  - Dachwald, Bernd
A1  - Ohndorf, Andreas
A1  - Dykta, Paul
A1  - Bowitz, Pascal
A1  - Rudolph, Martin
A1  - Digel, Ilya
A1  - Kowalski, Julia
A1  - Voigt, Konstantin
A1  - Förstner, Roger
T1  - A lander mission to probe subglacial water on Saturn's moon enceladus for life
JF  - Acta astronautica
Y1  - 2015
SN  - 1879-2030 (E-Journal); 0094-5765 (Print)
VL  - Vol. 106
SP  - 63
EP  - 89
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Dachwald, Bernd
A1  - Mikucki, Jill
A1  - Tulaczyk, Slawek
A1  - Digel, Ilya
A1  - Espe, Clemens
A1  - Feldmann, Marco
A1  - Francke, Gero
A1  - Kowalski, Julia
A1  - Xu, Changsheng
T1  - IceMole : A maneuverable probe for clean in situ analysis and sampling of subsurface ice and subglacial aquatic ecosystems
JF  - Annals of Glaciology
N2  - There is significant interest in sampling subglacial environments for geobiological studies, but they are difficult to access. Existing ice-drilling technologies make it cumbersome to maintain microbiologically clean access for sample acquisition and environmental stewardship of potentially fragile subglacial aquatic ecosystems. The IceMole is a maneuverable subsurface ice probe for clean in situ analysis and sampling of glacial ice and subglacial materials. The design is based on the novel concept of combining melting and mechanical propulsion. It can change melting direction by differential heating of the melting head and optional side-wall heaters. The first two prototypes were successfully tested between 2010 and 2012 on glaciers in Switzerland and Iceland. They demonstrated downward, horizontal and upward melting, as well as curve driving and dirt layer penetration. A more advanced probe is currently under development as part of the Enceladus Explorer (EnEx) project. It offers systems for obstacle avoidance, target detection, and navigation in ice. For the EnEx-IceMole, we will pay particular attention to clean protocols for the sampling of subglacial materials for biogeochemical analysis. We plan to use this probe for clean access into a unique subglacial aquatic environment at Blood Falls, Antarctica, with return of a subglacial brine sample.
KW  - Antarctic Glaciology
KW  - Extraterrestrial Glaciology
KW  - Glaciological instruments and methods
KW  - Subclacial exploration
KW  - Subglacial lakes
Y1  - 2014
U6  - http://dx.doi.org/10.3189/2014AoG65A004
SN  - 1727-5644
VL  - 55
IS  - 65
SP  - 14
EP  - 22
PB  - Cambridge University Press
CY  - Cambridge
ER  - 
TY  - RPRT
A1  - Blandford, Daniel
A1  - Dachwald, Bernd
A1  - Digel, Ilya
A1  - Espe, Clemens
A1  - Feldmann, Marco
A1  - Francke, Gero
A1  - Hiecke, Hannah
A1  - Kowalski, Julia
A1  - Lindner, Peter
A1  - Plescher, Engelbert
A1  - Schöngarth, Sarah
T1  - Enceladus Explorer : Schlussbericht —  Version: 1.0
Y1  - 2015
U6  - http://dx.doi.org/10.2314/GBV:86319950X
N1  - Förderkennzeichen BMWi 50NA1206
PB  - FH Aachen
CY  - Aachen
ER  - 
TY  - JOUR
A1  - Zhubanova, Azhar A.
A1  - Digel, Ilya
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology
N2  - The most of conventional methods of air purification use the power of a fan to draw in air and pass it through a filter. The problem of bacterial contamination of inner parts of such a type of air conditioners in some cases draws attention towards alternative air-cleaning systems. Some manufacturers offer to use the ozone's bactericidal and deodorizing effects, but the wide spreading of such systems is restricted by the fact that toxic effects of ozone in respect of human beings are well known. In 2000 Sharp Inc. introduced "Plasma Cluster Ions (PCI)" air purification technology, which uses plasma discharge to generate cluster ions (I 0-14 ). This technology has been developed for those customers that are conscious about health and hygiene. In our experiments, we focused on some principal aspects of plasma-generated ions application - time-dependency and irreversibility of bactericidal action, spatial and kinetic characteristics of emitted cluster particles, their chemical targets in the microbial cells.
KW  - Clusterion
KW  - Raumluft
KW  - Luftreiniger
KW  - Plasmacluster ion technology
KW  - Air purification
Y1  - 2007
ER  - 
TY  - CHAP
A1  - Bassam, Rasha
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Effect of nitric oxide on protein thermal stability : [abstract]
N2  - As a deduction from these results, we can conclude that proteins mainly in vitro, denaturate totally at a temperature between 57°C -62°C, and they also affected by NO and different ions types. In which mainly, NO cause earlier protein denaturation, which means that, NO has a destabilizing effect on proteins, and also different ions will alter the protein denaturation in which, some ions will cause earlier protein denaturation while others not.
KW  - Stickstoffmonoxid
KW  - Proteine
KW  - Hämoglobin
KW  - nitric oxide gas
KW  - protein
KW  - hemoglobin
Y1  - 2009
ER  - 
TY  - CHAP
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Zaccai, Joe
A1  - Büldt, Georg
T1  - Temperature transitions of hemoglobin and cytosolic water diffusion in human red blood cells : [poster]
N2  - Background Hemoglobin interactions in red blood cells Hemoglobin dynamics in human red blood cells Diffusion of H2O in red blood cells
KW  - Erythrozyt
KW  - Hämoglobin
KW  - cytosolic water diffusion
KW  - hemoglobin dynamics
Y1  - 2007
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Zerlin, Kay
A1  - Digel, Ilya
T1  - Hemoglobin Senses Body Temperature
JF  - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)
Y1  - 2008
SN  - 978-3-540-75408-4
SP  - 415
EP  - 447
PB  - Springer
CY  - Berlin
ER  - 
TY  - JOUR
A1  - Stadler, A.  M.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Embs, Jan P.
A1  - Zaccai, Joe
A1  - Büldt, Georg
T1  - Hemoglobin Dynamics in Red Blood Cells: Correlation to Body Temperature
JF  - Biophysical Journal. 95 (2008), H. 11
Y1  - 2008
SN  - 1542-0086
SP  - 5449
EP  - 5461
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Garvey, G. J.
A1  - Bocahut, A.
A1  - Sacquin-Mora, S.
A1  - Digel, Ilya
A1  - Schneider, G. J.
A1  - Natali, F.
A1  - Artmann, Gerhard
A1  - Zaccai, G.
T1  - Thermal fluctuations of haemoglobin from different species : adaptation to temperature via conformational dynamics
JF  - Journal of the Royal Society Interface
N2  - Thermodynamic stability, configurational motions and internal forces of haemoglobin (Hb) of three endotherms (platypus, Ornithorhynchus anatinus; domestic chicken, Gallus gallus domesticus and human, Homo sapiens) and an ectotherm (salt water crocodile, Crocodylus porosus) were investigated using circular dichroism, incoherent elastic neutron scattering and coarse-grained Brownian dynamics simulations. The experimental results from Hb solutions revealed a direct correlation between protein resilience, melting temperature and average body temperature of the different species on the 0.1 ns time scale. Molecular forces appeared to be adapted to permit conformational fluctuations with a root mean square displacement close to 1.2 Å at the corresponding average body temperature of the endotherms. Strong forces within crocodile Hb maintain the amplitudes of motion within a narrow limit over the entire temperature range in which the animal lives. In fully hydrated powder samples of human and chicken, Hb mean square displacements and effective force constants on the 1 ns time scale showed no differences over the whole temperature range from 10 to 300 K, in contrast to the solution case. A complementary result of the study, therefore, is that one hydration layer is not sufficient to activate all conformational fluctuations of Hb in the pico- to nanosecond time scale which might be relevant for biological function. Coarse-grained Brownian dynamics simulations permitted to explore residue-specific effects. They indicated that temperature sensing of human and chicken Hb occurs mainly at residues lining internal cavities in the β-subunits.
Y1  - 2012
U6  - http://dx.doi.org/10.1098/rsif.2012.0364
SN  - 1742-5689
VL  - 9
IS  - 76
SP  - 2845
EP  - 2855
PB  - The Royal Society
CY  - London
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Digel, Ilya
A1  - Embs, Jan P.
A1  - Unruh, Tobias
A1  - Tehei, M.
A1  - Zaccai, G.
A1  - Büldt, G.
A1  - Artmann, Gerhard
T1  - From powder to solution : Hydration dependence of human hemoglobin dynamics correlated to body temperature
JF  - Biophysical Journal. 96 (2009), H. 12
Y1  - 2009
SN  - 0006-3495
SP  - 5073
EP  - 5081
PB  - Cell Press
CY  - Cambridge, Mass.
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Embs, Jan P.
A1  - Digel, Ilya
A1  - Artmann, Gerhard
A1  - Unruh, Tobias
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
T1  - Cytoplasmic water and hydration layer dynamics in human red blood cells
JF  - Journal of the American Chemical Society. 50 (2008), H. 130
Y1  - 2008
SN  - 1520-5126
SP  - 16852
EP  - 16853
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Kayser, Peter
A1  - Artmann, Gerhard
T1  - Molecular processes in biological thermosensation
JF  - Journal of Biophysics. 2008 (2008)
Y1  - 2008
SN  - 1687-8000
SP  - 1
EP  - 9
ER  - 
TY  - JOUR
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Stadler, Andreas M.
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
A1  - Artmann, Gerhard
T1  - Dynamics and interactions of hemoglobin in human red blood cells and concentrated hemoglobin solutions
JF  - Regenerative medicine. 2 (2007), H. 5
Y1  - 2007
SN  - 1746-0751
N1  - Proceedings of the 3rd World Congress on Regenerative Medicine. October 18-20, 2007. Leipzig, Germany
SP  - 573
EP  - 573
ER  - 
TY  - JOUR
A1  - Maggakis-Kelemen, Christina
A1  - Digel, Ilya
A1  - Artmann, Gerhard
T1  - Polystyrene sulfonate/Polyallylamine hydrochloride microcapsules as potential artificial red blood cells - improvement of capsule flexibility
JF  - Biomedizinische Technik. 50 (2005), H. Erg.-Bd. 1
Y1  - 2005
SP  - 324
EP  - 326
ER  - 
TY  - JOUR
A1  - Stadler, Andreas M.
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Büldt, Georg
A1  - Zaccai, Guiseppe
A1  - Artmann, Gerhard
T1  - Dynamics and interactions of hemoglobin in red blood cells
JF  - Tissue Engineering Part A. 14 (2008), H. 5
Y1  - 2008
SN  - 1937-3341
N1  - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal
SP  - 724
EP  - 724
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Digel, Ilya
A1  - Linder, Peter
A1  - Porst, Dariusz
T1  - Mechanism of haemoglobin sensing body temperature
JF  - Tissue Engineering Part A. 14 (2008), H. 5
Y1  - 2008
SN  - 1937-3341
N1  - TERMIS EU 2008 Porto Meeting June 22–26, 2008 Porto Congress Center–Alfândega Portugal
SP  - 754
EP  - 754
ER  - 
TY  - JOUR
A1  - Artmann, Gerhard
A1  - Zerlin, Kay
A1  - Digel, Ilya
A1  - Stadler, Andreas M.
T1  - Physical discontinuities at body temperature in human red blood cells / Artmann, GM ; Zerlin, K ; Digel, I ; Stadler, A ; Zaccai, G ; Temiz, AA
JF  - Tissue Engineering. 13 (2007), H. 7
Y1  - 2007
SN  - 1076-3279
N1  - TERMIS-EU Meeting Abstracts London, UK September 4–7, 2007
SP  - 1778
EP  - 1778
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Zhubanova, Azhar A.
A1  - Nojima, H.
A1  - Artmann, Gerhard
T1  - The use of bactericidal effects of cluster ions generated by plasma in medical biotechnology
JF  - Biotechnologija : teorija i praktika (2004)
Y1  - 2004
SN  - 1028-9399
SP  - 46
EP  - 52
ER  - 
TY  - CHAP
A1  - Schlemmer, Katharina
A1  - Porst, Dariusz
A1  - Bassam, Rasha
A1  - Artmann, Gerhard
A1  - Digel, Ilya
ED  - Erni, Daniel
ED  - Fischerauer, Alice
ED  - Himmel, Jörg
ED  - Seeger, Thomas
ED  - Thelen, Klaus
T1  - Effects of nitric oxide (NO) and ATP on red blood cell phenotype and deformability
T2  - 2nd YRA MedTech Symposium 2017 : June 8th - 9th / 2017 / Hochschule Ruhr-West
Y1  - 2017
SN  - 978-3-9814801-9-1
U6  - http://dx.doi.org/10.17185/duepublico/43984
N1  - A young researchers track of the 7th IEEE Workshop & SENSORICA 2017
SP  - 100
EP  - 101
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  - 
TY  - BOOK
A1  - Staat, Manfred
A1  - Digel, Ilya
A1  - Trzewik, Jürgen
A1  - Sielemann, Stefanie
A1  - Erni, Daniel
A1  - Zylka, Waldemar
T1  - Symposium Proceedings; 4th YRA MedTech Symposium 2024 : February 1 / 2024 / FH Aachen
Y1  - 2024
SN  - 978-3-940402-65-3
U6  - http://dx.doi.org/10.17185/duepublico/81475
PB  - Universität Duisburg-Essen
CY  - Duisburg
ER  - 
TY  - JOUR
A1  - Bogoyavlenskiy, A. P.
A1  - Digel, Ilya
A1  - Berezin, V. E.
T1  - Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates
N2  - The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine- phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI)
KW  - Enzyme-linked immunosorbent assay
KW  - Peroxidase
KW  - ELISA
KW  - phenols
KW  - naphtols
KW  - aromatic amines
Y1  - 1997
ER  - 
TY  - CHAP
A1  - Arinkin, Vladimir
A1  - Digel, Ilya
T1  - Water bridge phenomenon : [abstract]
N2  - One of interesting but not well known water properties is related to appearance of highly ordered structures in response to strong electrical field. In 1893 Sir William Armstrong placed a cotton thread between two wine glasses filled with chemically pure water. When high DC voltage was applied between the glasses, a connection consisting of water formed, producing a "water bridge"
KW  - Hydrodynamik
KW  - Elektrodynamik
KW  - Wasserbrücke
KW  - water bridge phenomenon
Y1  - 2009
ER  - 
TY  - CHAP
A1  - ElBashir, Rasha
A1  - Digel, Ilya
T1  - Effect of nitric oxide gas on hydrogels : [abstract]
N2  - The results support our theory that the NO gas has an influence in increasing the translational diffusion of hydrogels and it accelerates the melting process of the gels.
KW  - Stickstoffmonoxid
KW  - Hydrogel
KW  - nitric oxide gas
KW  - hydrogel
Y1  - 2009
ER  - 
TY  - BOOK
A1  - Digel, Ilya
A1  - Zhubanova, Azhar Ahmetovna
A1  - Akimbekov, Nuraly Shardarbekovich
T1  - Visual Virology
Y1  - 2012
SN  - 978-601-247-298-1
N1  - Text kasachisch, russisch, englisch
CY  - Almaty
ER  - 
TY  - JOUR
A1  - Digel, Ilya
T1  - Primary thermosensory events in cells
JF  - Transient receptor potential channels / Md. Shahidul Islam, ed.
Y1  - 2011
SN  - 978-94-007-0264-6
N1  - (Advances in experimental medicine and biology ; 704)
SP  - 451
EP  - 468
PB  - Springer
CY  - Dordrecht [u.a.]
ER  - 
TY  - JOUR
A1  - Digel, Ilya
T1  - Controlling microbial adhesion : a surface engineering approach
JF  - Bioengineering in Cell and Tissue Research / Artmann, Gerhard M. ; Chien, Shu (Eds.)
Y1  - 2008
SN  - 978-3-540-75408-4
SP  - 601
EP  - 625
PB  - Springer
CY  - Berlin [u.a.]
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Kurulgan Demirci, Eylem
A1  - Linder, Peter
A1  - Kayser, Peter
T1  - Decrease in extracellular collagen crosslinking after NMR magnetic field application in skin fibroblasts
JF  - Medical and Biological Engineering and Computing. 45 (2007), H. 1
Y1  - 2007
SN  - 1741-0444
SP  - 91
EP  - 97
ER  - 
TY  - JOUR
A1  - Digel, Ilya
A1  - Maggakis-Kelemen, Christina
A1  - Zerlin, Kay
A1  - Linder, Peter
T1  - Body temperature-related structural transitions of monotremal and human hemoglobin
JF  - Biophysical Journal. 91 (2006), H. 8
Y1  - 2006
SN  - 1542-0086
SP  - 3014
EP  - 3021
ER  - 
TY  - JOUR
A1  - Bogoyavlenskiy, A. P.
A1  - Berezin, V. E.
A1  - Ogneva, A. V.
A1  - Tolmacheva, V. P.
A1  - Digel, Ilya
A1  - Khudyakova, S. S.
T1  - Immunostimulating activity of a saponin-containing extract of Saponaria officinalis
JF  - Voprosy virusologii
Y1  - 1999
SN  - 0507-4088
VL  - 44
IS  - 5
SP  - 229
EP  - 232
ER  - 
TY  - CHAP
A1  - Digel, Ilya
A1  - Mansurov, Zulkhair
A1  - Biisenbaev, Makhmut
A1  - Savitskaya, Irina
A1  - Kistaubaeva, Aida
A1  - Akimbekov, Nuraly
A1  - Zhubanova, Azhar
ED  - Hu, Ning
T1  - Heterogeneous Composites on the Basis of Microbial Cells and Nanostructured Carbonized Sorbents
T2  - Composites and Their Applications
N2  - The fact that microorganisms prefer to grow on liquid/solid phase surfaces rather than in the surrounding aqueous phase was noticed long time ago [1]. Virtually any surface – animal, mineral, or vegetable – is a subject for microbial colonization and subsequent biofilm formation. It would be adequate to name just a few notorious examples on microbial colonization of contact lenses, ship hulls, petroleum pipelines, rocks in streams and all kinds of biomedical implants. The propensity of microorganisms to become surface-bound is so profound and ubiquitous that it vindicates the advantages for attached forms over their free-ranging counterparts [2]. Indeed, from ecological and evolutionary standpoints, for many microorganisms the surface-bound state means dwelling in nutritionally favorable, non-hostile environments [3]. Therefore, in most of natural and artificial ecosystems surface-associated microorganisms vastly outnumber organisms in suspension and often organize into complex communities with features that differ dramatically from those of free cells [4].
Y1  - 2012
SN  - 978-953-51-0706-4
U6  - http://dx.doi.org/10.5772/47796
SP  - 249
EP  - 272
PB  - Intech
CY  - London
ER  - 
TY  - BOOK
A1  - Digel, Ilya
T1  - Enhancement of microbial adhesion onto solid surfaces using transition metals and water-soluble polymers : academic PhD dissertation in microbiology, Division of Applied Microbiology, Department of Microbiology, Biology Faculty, Kazakh National State University, Almaty, Kazakhstan / Ilya Digel
Y1  - 1998
CY  - Almaty
ER  -